##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211288_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6578689 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.340102412501945 31.0 31.0 34.0 28.0 34.0 2 31.353674417501725 31.0 31.0 34.0 28.0 34.0 3 31.40319431424711 31.0 31.0 34.0 28.0 34.0 4 35.00529801606368 37.0 35.0 37.0 32.0 37.0 5 35.048175251938495 37.0 35.0 37.0 32.0 37.0 6 35.11464579036948 37.0 35.0 37.0 32.0 37.0 7 35.04513391649917 37.0 35.0 37.0 32.0 37.0 8 35.04611222691938 37.0 35.0 37.0 32.0 37.0 9 36.59814531436279 39.0 37.0 39.0 32.0 39.0 10-11 36.53064181936553 39.0 37.0 39.0 32.0 39.0 12-13 36.44585167348692 39.0 37.0 39.0 32.0 39.0 14-15 37.569370204306665 40.0 37.0 41.0 32.0 41.0 16-17 37.56650291570251 40.0 37.0 41.0 32.0 41.0 18-19 37.568313534809135 40.0 37.0 41.0 32.0 41.0 20-21 37.53466442934147 40.0 37.0 41.0 31.5 41.0 22-23 37.41330354725691 40.0 37.0 41.0 31.0 41.0 24-25 37.30074054268259 40.0 37.0 41.0 31.0 41.0 26-27 37.15162983080671 40.0 36.0 41.0 30.5 41.0 28-29 37.012902874721696 40.0 36.0 41.0 30.0 41.0 30-31 36.82245710353537 39.0 36.0 41.0 30.0 41.0 32-33 36.69357169794772 39.0 35.5 41.0 30.0 41.0 34-35 36.54479760329148 39.0 35.0 41.0 30.0 41.0 36-37 36.44120933517301 39.0 35.0 41.0 29.0 41.0 38-39 36.2857165766614 39.0 35.0 41.0 29.0 41.0 40-41 36.11459198025625 39.0 35.0 40.0 28.0 41.0 42-43 35.95861637478227 39.0 35.0 40.0 27.0 41.0 44-45 35.81126931216843 38.5 35.0 40.0 27.0 41.0 46-47 35.63431954299709 38.0 34.5 40.0 26.0 41.0 48-49 35.389887559664245 38.0 34.0 40.0 25.5 41.0 50-51 34.719807700288 37.5 33.5 39.5 24.5 40.5 52-53 34.95472160182675 38.0 33.5 40.0 25.5 40.5 54-55 35.310866040331135 38.0 34.0 40.0 26.0 41.0 56-57 35.20741822572856 38.0 34.0 40.0 26.0 41.0 58-59 34.98090105794635 38.0 34.0 40.0 25.0 41.0 60-61 34.71346433917152 37.5 33.5 40.0 25.0 41.0 62-63 34.44138246997236 37.0 33.0 40.0 24.5 41.0 64-65 34.165322148531416 36.5 33.0 40.0 24.0 41.0 66-67 33.938726241656965 36.0 33.0 39.0 23.5 41.0 68-69 33.600211151492346 35.5 32.5 39.0 23.0 41.0 70-71 33.25762876159672 35.0 32.0 39.0 23.0 40.5 72-73 32.90380826635824 35.0 32.0 37.5 22.0 40.0 74-75 32.53948978284275 35.0 31.0 37.0 21.5 39.5 76-77 32.18942573512747 35.0 31.0 36.5 21.0 39.0 78-79 31.830797671086138 35.0 31.0 36.0 20.0 39.0 80-81 31.501048613181137 35.0 30.5 36.0 20.0 37.5 82-83 31.19169320817567 34.0 30.0 35.0 19.5 37.0 84-85 30.89168981844255 34.0 30.0 35.0 19.0 37.0 86-87 30.638603223225783 34.0 30.0 35.0 18.0 36.0 88-89 30.38100448280805 34.0 29.0 35.0 18.0 36.0 90-91 30.125820585226023 34.0 29.0 35.0 17.5 36.0 92-93 29.892056988862066 34.0 29.0 35.0 16.5 35.0 94-95 29.66898488437438 34.0 29.0 35.0 15.0 35.0 96-97 29.467856969678913 34.0 28.5 35.0 14.0 35.0 98-99 29.230976262899794 34.0 27.0 35.0 11.5 35.0 100 27.558139167241375 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.372378469935537 1101 2 -0.3861344015412236 1101 3 -0.38337082442108183 1101 4 -0.23707062545263824 1101 5 -0.25560988997317935 1101 6 -0.3012394356023833 1101 7 -0.39189902393514586 1101 8 -0.49507782568861813 1101 9 -0.44521070223765236 1101 10-11 -0.5384142878699052 1101 12-13 -0.4671105551445791 1101 14-15 -0.6092619773328636 1101 16-17 -0.6224284559282083 1101 18-19 -0.6813645517571842 1101 20-21 -0.6402523551104622 1101 22-23 -0.6209547226516108 1101 24-25 -0.6404133805766463 1101 26-27 -0.7038388737400965 1101 28-29 -0.746389541770256 1101 30-31 -0.7528364047873524 1101 32-33 -0.7655598014054803 1101 34-35 -0.817346532812067 1101 36-37 -0.8703909941698029 1101 38-39 -0.7970469802896005 1101 40-41 -0.8211267205350126 1101 42-43 -0.8822026221481707 1101 44-45 -0.9389986522464824 1101 46-47 -0.8865823321938748 1101 48-49 -0.7518624769658686 1101 50-51 -0.7583375852387633 1101 52-53 -0.6585644737069245 1101 54-55 -0.6160457814318931 1101 56-57 -0.5562376662645292 1101 58-59 -0.6160641257143666 1101 60-61 -0.628225654391585 1101 62-63 -0.6652305315807681 1101 64-65 -0.6865676098030491 1101 66-67 -0.7841040497546672 1101 68-69 -0.8062971758522792 1101 70-71 -0.8173879521531546 1101 72-73 -0.9333691985037333 1101 74-75 -0.9220162495375135 1101 76-77 -0.729736979003782 1101 78-79 -0.8465795432540659 1101 80-81 -0.9519029232284417 1101 82-83 -0.905824568159165 1101 84-85 -0.8463253123864298 1101 86-87 -0.9632320755723214 1101 88-89 -1.05831767651798 1101 90-91 -0.9330677254374038 1101 92-93 -0.8700246120771418 1101 94-95 -0.9641782216720642 1101 96-97 -1.0811870762054667 1101 98-99 -1.0707298991863397 1101 100 -1.1174710573815894 1102 1 -0.3476667263022932 1102 2 -0.3747803065358113 1102 3 -0.2965418789186245 1102 4 -0.18995603333521416 1102 5 -0.2016146251086326 1102 6 -0.22019724709416977 1102 7 -0.26699024035197993 1102 8 -0.2331915615894573 1102 9 -0.3152432466428863 1102 10-11 -0.3519827064557006 1102 12-13 -0.39282253994000627 1102 14-15 -0.4521395207622092 1102 16-17 -0.45602013406161745 1102 18-19 -0.4710830777929971 1102 20-21 -0.5555597216500701 1102 22-23 -0.5985836090216523 1102 24-25 -0.6128233594681447 1102 26-27 -0.5566347654900738 1102 28-29 -0.6820560804377749 1102 30-31 -0.6265930258190409 1102 32-33 -0.6518720421689039 1102 34-35 -0.7686408550208697 1102 36-37 -0.7603628767483457 1102 38-39 -0.9057706901769436 1102 40-41 -0.8256164218084123 1102 42-43 -0.7405267368564452 1102 44-45 -0.8770981017858475 1102 46-47 -0.8599254094710318 1102 48-49 -0.7754112432180591 1102 50-51 -0.7892321186802036 1102 52-53 -0.7288537127755177 1102 54-55 -0.5870483280545997 1102 56-57 -0.5330223714500022 1102 58-59 -0.6721579358736349 1102 60-61 -0.7730440140777262 1102 62-63 -0.7334381430407859 1102 64-65 -0.8088085367287547 1102 66-67 -0.7858993966188947 1102 68-69 -0.7868238120678654 1102 70-71 -0.7704361359941316 1102 72-73 -0.8712014990445027 1102 74-75 -0.8346204622003235 1102 76-77 -0.8979743695653717 1102 78-79 -0.8420099797156055 1102 80-81 -0.8544501533774387 1102 82-83 -0.9669706636196516 1102 84-85 -0.9310051724260724 1102 86-87 -0.9662814743075359 1102 88-89 -1.0189562243628423 1102 90-91 -1.1757994929120947 1102 92-93 -1.1825376480626524 1102 94-95 -1.2040656463926211 1102 96-97 -1.2610511039931644 1102 98-99 -1.1065633041454461 1102 100 -1.2214547069488653 1103 1 -0.44287841543484774 1103 2 -0.5042517357450151 1103 3 -0.4799460443922605 1103 4 -0.38854390530185157 1103 5 -0.21631166483874864 1103 6 -0.46773680785993577 1103 7 -0.446326019383001 1103 8 -0.3961121137858825 1103 9 -0.594005341842049 1103 10-11 -0.5650380612614541 1103 12-13 -0.6770311881185904 1103 14-15 -0.7785971545715142 1103 16-17 -0.6907352787575647 1103 18-19 -0.7028566550605575 1103 20-21 -0.8302841722604128 1103 22-23 -0.8131989240929158 1103 24-25 -0.8367946318631212 1103 26-27 -0.8023169756184458 1103 28-29 -0.8312071493200079 1103 30-31 -0.8892689634903306 1103 32-33 -0.8394761211066069 1103 34-35 -1.0059938689589316 1103 36-37 -1.0669822664159696 1103 38-39 -1.0437097658088348 1103 40-41 -0.9506027991671004 1103 42-43 -0.9940574905146704 1103 44-45 -0.948374544395584 1103 46-47 -0.9808613867492468 1103 48-49 -1.0189585309026228 1103 50-51 -1.0021254144049792 1103 52-53 -0.8545105786370826 1103 54-55 -0.8210543960881367 1103 56-57 -0.9032463489323064 1103 58-59 -0.8604132302138723 1103 60-61 -0.8409113277611056 1103 62-63 -0.7712373222584645 1103 64-65 -0.7728311969715733 1103 66-67 -0.8517505454019769 1103 68-69 -0.813581560777294 1103 70-71 -0.8544770494574152 1103 72-73 -1.0125228639223884 1103 74-75 -0.9164526895542053 1103 76-77 -0.8556311443482585 1103 78-79 -0.8197402059901933 1103 80-81 -0.9900952125769074 1103 82-83 -1.0547418031337337 1103 84-85 -0.9564291330800785 1103 86-87 -1.0087410234546148 1103 88-89 -1.1293763337534912 1103 90-91 -1.1844485134749547 1103 92-93 -1.1959173196355941 1103 94-95 -1.2811658520805196 1103 96-97 -1.385893954098126 1103 98-99 -1.2532078349990243 1103 100 -1.3223071765966665 1104 1 -0.40899462260182773 1104 2 -0.36956070695368837 1104 3 -0.4426628356381599 1104 4 -0.2192284866586789 1104 5 -0.2028102327466499 1104 6 -0.2394905920584094 1104 7 -0.2735869420179782 1104 8 -0.24709531549724062 1104 9 -0.3819656438037953 1104 10-11 -0.35717819188166544 1104 12-13 -0.41952395287525235 1104 14-15 -0.555450555725514 1104 16-17 -0.5340654391602655 1104 18-19 -0.5852613206574162 1104 20-21 -0.5382090041283689 1104 22-23 -0.49251507330727406 1104 24-25 -0.6498618055412635 1104 26-27 -0.6785543959674953 1104 28-29 -0.6335276948635595 1104 30-31 -0.7257255502634123 1104 32-33 -0.7549792360599668 1104 34-35 -0.657664843037125 1104 36-37 -0.762494209035971 1104 38-39 -0.7731744083413474 1104 40-41 -0.807763066515065 1104 42-43 -0.6865558886407896 1104 44-45 -0.77585681612846 1104 46-47 -0.7755618447573909 1104 48-49 -0.8174070491785272 1104 50-51 -0.8001854726156381 1104 52-53 -0.59372768902508 1104 54-55 -0.6776152910041375 1104 56-57 -0.5575740052591343 1104 58-59 -0.6306711925152513 1104 60-61 -0.6255175766018723 1104 62-63 -0.6851499451232357 1104 64-65 -0.731075972175482 1104 66-67 -0.6512062493262007 1104 68-69 -0.692418672248408 1104 70-71 -0.741155392114365 1104 72-73 -0.7100974169036363 1104 74-75 -0.6728439752616957 1104 76-77 -0.6636948915735843 1104 78-79 -0.7031857506195038 1104 80-81 -0.709267923302523 1104 82-83 -0.798430148863357 1104 84-85 -0.8745030532420444 1104 86-87 -0.9301229354161009 1104 88-89 -0.856573699160954 1104 90-91 -0.8005190167990541 1104 92-93 -0.9334925349794609 1104 94-95 -0.8381581702354737 1104 96-97 -0.9479364174592391 1104 98-99 -0.9497460287920276 1104 100 -1.0786889706313119 1105 1 -0.42002036498426776 1105 2 -0.4621201244427411 1105 3 -0.4293597121651729 1105 4 -0.39928582191385686 1105 5 -0.39566529930249317 1105 6 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-0.7807221433705038 1105 68-69 -0.6221632708712832 1105 70-71 -0.5850056985057961 1105 72-73 -0.6443446103187043 1105 74-75 -0.7091598911943322 1105 76-77 -0.8026493489214062 1105 78-79 -0.6453395433218461 1105 80-81 -0.7251619813298475 1105 82-83 -0.7329705504605464 1105 84-85 -0.8203917289676639 1105 86-87 -0.7553844910350094 1105 88-89 -0.7571581963144105 1105 90-91 -0.8802001455563797 1105 92-93 -0.8741982707921103 1105 94-95 -0.8552247589695554 1105 96-97 -0.9899764687798687 1105 98-99 -0.9183285620354908 1105 100 -1.016668974102366 1106 1 -0.4191227485932707 1106 2 -0.4535251916166203 1106 3 -0.39833275122138545 1106 4 -0.3716699268315722 1106 5 -0.3415704863179556 1106 6 -0.3294523382958161 1106 7 -0.3830354670752385 1106 8 -0.34914405929951187 1106 9 -0.4313578691463178 1106 10-11 -0.43351607759070276 1106 12-13 -0.39375490848166805 1106 14-15 -0.5284529457052969 1106 16-17 -0.5659132382073366 1106 18-19 -0.5730052750215151 1106 20-21 -0.4910113794735338 1106 22-23 -0.5630984831067352 1106 24-25 -0.5692662525606522 1106 26-27 -0.4912505445532318 1106 28-29 -0.6288746188177043 1106 30-31 -0.7318789968252162 1106 32-33 -0.7013261982404586 1106 34-35 -0.6779217939545603 1106 36-37 -0.6744244382719913 1106 38-39 -0.7172517553406763 1106 40-41 -0.6856326430720259 1106 42-43 -0.7413371412164338 1106 44-45 -0.6958376690855914 1106 46-47 -0.768494245583021 1106 48-49 -0.8034028876147516 1106 50-51 -0.7495974106003658 1106 52-53 -0.5923684091986203 1106 54-55 -0.6616753092656822 1106 56-57 -0.6011663373881007 1106 58-59 -0.5618573236754756 1106 60-61 -0.6010395180656758 1106 62-63 -0.5155474442494281 1106 64-65 -0.5471992925080968 1106 66-67 -0.6767508147633876 1106 68-69 -0.7075809683429881 1106 70-71 -0.6807690743278556 1106 72-73 -0.5740769329602813 1106 74-75 -0.5634055847552482 1106 76-77 -0.6410891442563802 1106 78-79 -0.5196618043963923 1106 80-81 -0.6696422731791536 1106 82-83 -0.6877268481342433 1106 84-85 -0.639803953432093 1106 86-87 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1.1751753529263915 2212 92-93 1.2188025903691333 2212 94-95 1.2129946671963374 2212 96-97 1.2144871470231777 2212 98-99 1.1873548223098886 2212 100 1.2802864372051843 2213 1 0.47584774218776005 2213 2 0.5268342858909918 2213 3 0.5037395617041369 2213 4 0.3773518539456333 2213 5 0.4213651263875988 2213 6 0.4415674265160803 2213 7 0.4779855547186571 2213 8 0.5001201646671802 2213 9 0.611957188114495 2213 10-11 0.6263639018652754 2213 12-13 0.6359944996034415 2213 14-15 0.839799044828105 2213 16-17 0.7772671234863964 2213 18-19 0.7443802726460547 2213 20-21 0.7689513803845713 2213 22-23 0.7693486758132551 2213 24-25 0.8156920653863224 2213 26-27 0.8470533031111458 2213 28-29 0.8461669290843474 2213 30-31 0.893539147342004 2213 32-33 0.9275315726691247 2213 34-35 0.9460387725561716 2213 36-37 0.9479718500943974 2213 38-39 0.9789909639727199 2213 40-41 1.0200599924895286 2213 42-43 1.0264475245199236 2213 44-45 1.0322664109585489 2213 46-47 1.0027169051467908 2213 48-49 0.9883475445595451 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0.36207851550567227 2311 8 0.4333515462681419 2311 9 0.6043075881366846 2311 10-11 0.6204283238104225 2311 12-13 0.6481329552363917 2311 14-15 0.7993848537074655 2311 16-17 0.7801827017054492 2311 18-19 0.6308175535765059 2311 20-21 0.7018241952799755 2311 22-23 0.8263146397447372 2311 24-25 0.8595121403943793 2311 26-27 0.9332114074883151 2311 28-29 0.9432205410036545 2311 30-31 0.9717017794631246 2311 32-33 0.9515505614800546 2311 34-35 0.9801101908557897 2311 36-37 0.9995401523765466 2311 38-39 1.0393617148515446 2311 40-41 1.0132615206898095 2311 42-43 1.0988381111181766 2311 44-45 1.0648668768679173 2311 46-47 1.1398353416236304 2311 48-49 1.0658036145870682 2311 50-51 1.0638673903860294 2311 52-53 0.9611989376902912 2311 54-55 0.890435850751139 2311 56-57 0.90753667918964 2311 58-59 0.9704633765796231 2311 60-61 0.9745087546497473 2311 62-63 0.9093273932167634 2311 64-65 0.9458191903114823 2311 66-67 0.8889393979398861 2311 68-69 0.9106195461349103 2311 70-71 0.8143690315974368 2311 72-73 0.8266572532800751 2311 74-75 0.9036863226196061 2311 76-77 0.9014273649637872 2311 78-79 0.9327391854309042 2311 80-81 1.0020655011417823 2311 82-83 0.9248939589186627 2311 84-85 1.0084197672604773 2311 86-87 1.0728726091940715 2311 88-89 1.0079169850815326 2311 90-91 1.0076042184348175 2311 92-93 1.040136606814901 2311 94-95 1.1126762981396965 2311 96-97 1.0311319565745514 2311 98-99 1.111170477005416 2311 100 1.239919829982778 2312 1 0.42470711290686936 2312 2 0.42877262980479003 2312 3 0.46776524077302284 2312 4 0.41452024186600767 2312 5 0.3245013917402133 2312 6 0.34897163916671303 2312 7 0.4225689581191432 2312 8 0.415167871776994 2312 9 0.516857857998815 2312 10-11 0.5541241367122183 2312 12-13 0.5689310700729067 2312 14-15 0.7887496087124717 2312 16-17 0.765764918836318 2312 18-19 0.658129687067877 2312 20-21 0.7124304321339849 2312 22-23 0.7505612849518641 2312 24-25 0.6994944204135152 2312 26-27 0.7283272170106585 2312 28-29 0.829680912517432 2312 30-31 0.8631251810154055 2312 32-33 0.8866931610985063 2312 34-35 0.8575941755239214 2312 36-37 0.8606062569541635 2312 38-39 0.8983073961842862 2312 40-41 0.8907327614239406 2312 42-43 0.8615551837035511 2312 44-45 0.9135904586290664 2312 46-47 0.9806578298479067 2312 48-49 0.9670645963833806 2312 50-51 0.9323698396955606 2312 52-53 0.8515019464157589 2312 54-55 0.8269497558840229 2312 56-57 0.7015707006359833 2312 58-59 0.6740126649859945 2312 60-61 0.7890474475256397 2312 62-63 0.7855887152151766 2312 64-65 0.7422301783078424 2312 66-67 0.7791770314664817 2312 68-69 0.7799705995461323 2312 70-71 0.7534429301867931 2312 72-73 0.7582451321742525 2312 74-75 0.7518619697967779 2312 76-77 0.8198969420187225 2312 78-79 0.7624644451342704 2312 80-81 0.8467191197422714 2312 82-83 0.8472053090355871 2312 84-85 0.8413233331751222 2312 86-87 0.8779463704654944 2312 88-89 0.8833810243741134 2312 90-91 0.9650008377501997 2312 92-93 1.0531261230599007 2312 94-95 1.054072785522166 2312 96-97 1.0589886772068517 2312 98-99 1.1419590210656274 2312 100 1.281556581909303 2313 1 0.43427888334097275 2313 2 0.43671159924267045 2313 3 0.48641344478455295 2313 4 0.39683135759418775 2313 5 0.3581058431515842 2313 6 0.3510167955999748 2313 7 0.4278315845251157 2313 8 0.37626211706797363 2313 9 0.45449739104621756 2313 10-11 0.4925507960627229 2313 12-13 0.5455632453878252 2313 14-15 0.709437495319726 2313 16-17 0.6957445223249508 2313 18-19 0.6261662945972901 2313 20-21 0.6195889218218085 2313 22-23 0.5555645175220789 2313 24-25 0.5898493561336835 2313 26-27 0.7002338818587717 2313 28-29 0.7261478107957089 2313 30-31 0.7727966812041203 2313 32-33 0.7542987965505716 2313 34-35 0.7828132006816944 2313 36-37 0.7911328509611693 2313 38-39 0.8380964241390885 2313 40-41 0.900799396832177 2313 42-43 0.7641953805323993 2313 44-45 0.8694294528635069 2313 46-47 0.8539266762950106 2313 48-49 0.9284897536793082 2313 50-51 0.9047790769327264 2313 52-53 0.7880081836194464 2313 54-55 0.7600501109004867 2313 56-57 0.7730642445487135 2313 58-59 0.7752625979611949 2313 60-61 0.803847895038075 2313 62-63 0.7590579666843169 2313 64-65 0.798521999772376 2313 66-67 0.7824760235183632 2313 68-69 0.7125306490469612 2313 70-71 0.8000166714121377 2313 72-73 0.8187522454715719 2313 74-75 0.8929498352357328 2313 76-77 0.8593270630995917 2313 78-79 0.8329802746265358 2313 80-81 0.7890565668660834 2313 82-83 0.7419459298806181 2313 84-85 0.862625063823586 2313 86-87 0.9148758199002032 2313 88-89 0.9179205372668875 2313 90-91 0.9545024822434023 2313 92-93 0.9682136551750027 2313 94-95 0.9813738525989066 2313 96-97 0.9887348998220205 2313 98-99 0.9423540846289065 2313 100 0.9580007539745985 2314 1 0.3319686734344316 2314 2 0.26511192249679993 2314 3 0.2650497407707242 2314 4 0.22446374365198096 2314 5 0.24938083310205172 2314 6 0.2438383006233522 2314 7 0.32882283691231606 2314 8 0.3298213202827114 2314 9 0.4141168441569789 2314 10-11 0.4427928889245649 2314 12-13 0.4405692194690758 2314 14-15 0.5506434251691275 2314 16-17 0.5349121359889679 2314 18-19 0.558475503028049 2314 20-21 0.5455502690292988 2314 22-23 0.5609761032059879 2314 24-25 0.6568342463677652 2314 26-27 0.6242324756198556 2314 28-29 0.6747306530118067 2314 30-31 0.6827846287212296 2314 32-33 0.7010514765415081 2314 34-35 0.7084523369644629 2314 36-37 0.6706803160451287 2314 38-39 0.6738323836572206 2314 40-41 0.7120711806691915 2314 42-43 0.6233753318217339 2314 44-45 0.7268399563971855 2314 46-47 0.5630310996188328 2314 48-49 0.5212045041657873 2314 50-51 0.7018632175899953 2314 52-53 0.5490782465872357 2314 54-55 0.5503821488063423 2314 56-57 0.5292616062163873 2314 58-59 0.4546383516760457 2314 60-61 0.3386600279172143 2314 62-63 0.5350240778564768 2314 64-65 0.5508653487908575 2314 66-67 0.5044505582146357 2314 68-69 0.5465803389372041 2314 70-71 0.6080747264070681 2314 72-73 0.6606813590681355 2314 74-75 0.6754500890820196 2314 76-77 0.625258525991434 2314 78-79 0.6468922685509142 2314 80-81 0.6681422403376835 2314 82-83 0.6774369103393205 2314 84-85 0.7192730548214286 2314 86-87 0.7265002414436168 2314 88-89 0.5844172080553669 2314 90-91 0.5109628703987248 2314 92-93 0.760022904638447 2314 94-95 0.8345967616356944 2314 96-97 0.8663124133523752 2314 98-99 0.8101691217878297 2314 100 0.876449676623114 2315 1 0.3537896170060364 2315 2 0.37534226097647405 2315 3 0.4145634454568423 2315 4 0.31799567591070144 2315 5 0.2820612384615018 2315 6 0.2928838468837469 2315 7 0.29608348666633333 2315 8 0.2505347801208444 2315 9 0.1305182588342575 2315 10-11 0.3555924309681373 2315 12-13 0.3962250946872814 2315 14-15 0.51734636561212 2315 16-17 0.35000599438522073 2315 18-19 0.5416212329244416 2315 20-21 0.4715489314337944 2315 22-23 0.40571017108329244 2315 24-25 0.614911396155783 2315 26-27 0.6118908900024067 2315 28-29 0.5734196960642493 2315 30-31 0.595049699341736 2315 32-33 0.6055664281386868 2315 34-35 0.5020063735556377 2315 36-37 0.6418539994204124 2315 38-39 0.7085492214907632 2315 40-41 0.7701381284576243 2315 42-43 0.7816293162693455 2315 44-45 0.7508996721014611 2315 46-47 0.7733492886505431 2315 48-49 0.7857899629157004 2315 50-51 0.6698601116478997 2315 52-53 0.32328815839661473 2315 54-55 0.4840368451268233 2315 56-57 0.46987293270304775 2315 58-59 0.56552445201838 2315 60-61 0.5979070069917825 2315 62-63 0.6557811795817443 2315 64-65 0.6984157353332847 2315 66-67 0.5820259963338401 2315 68-69 0.5926990448702298 2315 70-71 0.5136835590716089 2315 72-73 0.5387822001663238 2315 74-75 0.2134151302300893 2315 76-77 0.47014505708485643 2315 78-79 0.5835333168630541 2315 80-81 0.575964906913665 2315 82-83 0.5217645985662145 2315 84-85 0.5379670855181153 2315 86-87 0.41821678455273315 2315 88-89 0.48995576027827425 2315 90-91 0.655990529153172 2315 92-93 0.7153664853135915 2315 94-95 0.6981098768729481 2315 96-97 0.748415272179372 2315 98-99 0.9128086057933622 2315 100 1.0229526578741037 2316 1 0.14373354599874943 2316 2 0.1734135909995409 2316 3 0.22639987201447553 2316 4 0.11287559056052743 2316 5 0.1255476861408411 2316 6 -0.02009558403972278 2316 7 0.06631701422338665 2316 8 0.07630690420338482 2316 9 0.12733617949271547 2316 10-11 0.16069646519591885 2316 12-13 0.145685583401999 2316 14-15 0.05476820418338946 2316 16-17 0.1819714534661685 2316 18-19 0.27939277868692614 2316 20-21 0.3103784979781139 2316 22-23 0.22824180995256427 2316 24-25 0.17780477284941298 2316 26-27 0.10736240071623371 2316 28-29 0.19001526328213458 2316 30-31 -0.36777552432045724 2316 32-33 -0.1819380129002468 2316 34-35 -0.2709187918976923 2316 36-37 -0.030557273161228693 2316 38-39 0.048466469989207894 2316 40-41 0.26100789201448293 2316 42-43 0.23775167173130285 2316 44-45 0.09176055358045687 2316 46-47 0.024293769582605762 2316 48-49 0.16213517373699915 2316 50-51 0.2830349568496402 2316 52-53 -0.17049659690856345 2316 54-55 -0.006934604089103402 2316 56-57 0.018660096533103854 2316 58-59 -0.29206009731166915 2316 60-61 0.160799895470916 2316 62-63 0.02470050222095921 2316 64-65 -0.23837000256781948 2316 66-67 -0.5240594346944718 2316 68-69 -0.5304807201632684 2316 70-71 -0.37916100645003326 2316 72-73 -0.5463452615054152 2316 74-75 -0.3820668813781083 2316 76-77 -0.054302660966293104 2316 78-79 0.03424065402467491 2316 80-81 -0.04879501146372078 2316 82-83 0.14869646335032627 2316 84-85 0.28799747472956483 2316 86-87 0.37692531501338067 2316 88-89 0.39367365931825304 2316 90-91 -0.21872064486052167 2316 92-93 -0.2925690731130075 2316 94-95 0.10999429405960726 2316 96-97 0.3630822550719941 2316 98-99 0.3931339382331771 2316 100 0.28150459995713106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 184.0 1 477.0 2 304.0 3 276.0 4 245.0 5 233.0 6 279.0 7 800.0 8 2190.0 9 3674.0 10 5680.0 11 9147.0 12 13824.0 13 17331.0 14 20126.0 15 22978.0 16 26665.0 17 32205.0 18 38289.0 19 45609.0 20 54195.0 21 64631.0 22 72283.0 23 73920.0 24 73981.0 25 76322.0 26 83175.0 27 94539.0 28 110170.0 29 130517.0 30 157074.0 31 193136.0 32 242375.0 33 313789.0 34 429281.0 35 601915.0 36 881889.0 37 1259122.0 38 1146890.0 39 278738.0 40 231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36829794583865 18.985012820263314 13.384084263430868 23.262604970467173 2 15.850171895771753 19.94733561553782 40.553828211358756 23.648664277331672 3 19.059705524766713 25.57332850482372 29.48423885372155 25.88272711668802 4 12.828535878894098 16.317771024806323 37.87551409985168 32.9781789964479 5 13.552134059662144 37.42374231645779 34.47668292949918 14.547440694380883 6 31.133973396904757 36.57570500848502 17.930620628888082 14.359700965722134 7 27.70888964825765 32.25392460076492 22.054319604321222 17.98286614665621 8 26.04283455687296 35.23777401907107 20.050427766201416 18.668963657854558 9 26.53768289022435 16.002162857185436 20.51954493630793 36.94060931628228 10-11 25.1931574294255 25.999826344590115 27.920109995952096 20.88690623003229 12-13 25.691389685912807 23.691868521173788 28.21164609770931 22.405095695204096 14-15 23.14588678001316 25.16426301057925 25.911573798688934 25.778276410718654 16-17 22.342489527129104 28.054566436298227 26.738867599403125 22.86407643716954 18-19 22.12138786863379 27.1227853232895 28.92466548524218 21.83116132283453 20-21 23.067673735309146 26.34180262549578 28.800931333187336 21.789592306007737 22-23 23.033928978784886 26.08774998099004 28.574587483841533 22.303733556383545 24-25 22.675065112163285 26.271241547967765 28.765099195989798 22.288594143879152 26-27 22.502770615964604 26.462951010764357 28.583783099763544 22.4504952735075 28-29 22.33071465136743 26.50734154606667 28.813199604946867 22.348744197619038 30-31 22.789783994553726 26.332545840011846 28.70313924914214 22.17453091629229 32-33 22.672334230007284 26.581937086427576 28.416038059650084 22.329690623915052 34-35 22.805863374424767 26.553136574097834 28.55612439220363 22.084875659273767 36-37 22.788774009692837 26.462342552224076 28.312619088532674 22.43626434955041 38-39 22.69371029872241 26.560677467087 28.464004428446078 22.28160780574451 40-41 22.683486705964697 26.273966723315123 28.679680805663345 22.362865765056835 42-43 22.576179276006474 26.42178412575821 28.337780399181163 22.66425619905415 44-45 22.519618284176975 26.762725305692975 28.255181122156426 22.462475287973625 46-47 22.608751135325644 26.575536923569608 27.936809768968125 22.878902172136623 48-49 22.809540906083857 26.569347151250465 27.922123600915356 22.69898834175032 50-51 22.695188088061546 26.763330092089895 27.77137530595303 22.770106513895534 52-53 22.608658221471302 26.980243876766913 27.628881911053487 22.7822159907083 54-55 22.524193234410966 26.877454606508632 27.723366150641667 22.874986008438736 56-57 22.768027836002656 26.845246949011436 27.46561944018341 22.921105774802506 58-59 22.529537777778454 26.75919288311661 27.792297444564838 22.918971894540096 60-61 22.64118533104696 26.973652643887036 27.61990209231129 22.765259932754713 62-63 22.62669168955687 26.902942815689592 27.565014178987173 22.90535131576636 64-65 22.4185504301608 26.74430992717665 27.660546534436627 23.176593108225923 66-67 22.57429389602345 26.674550752356822 27.517542622958054 23.23361272866168 68-69 22.391223374200536 26.780935540503975 27.625286450399877 23.20255463489561 70-71 22.485425523015735 26.562233358935945 27.658296386989818 23.294044731058502 72-73 22.607462732764088 26.782126267327854 27.475662612926094 23.134748386981965 74-75 22.649364996587998 26.974729286898473 27.34074955146663 23.035156165046896 76-77 22.577060888291367 26.879490275758442 27.25944136559133 23.28400747035886 78-79 22.551595813974675 26.81454496948942 27.303610187894158 23.33024902864175 80-81 22.467712073640616 27.092533308895973 27.239917972601514 23.1998366448619 82-83 22.790202796420036 26.828717447694878 27.471497722372824 22.909582033512265 84-85 22.847275872298592 26.932153713118456 27.14097106952981 23.079599345053133 86-87 22.48872970750395 27.115758728972907 27.32058672288746 23.07492484063568 88-89 22.6690050038235 26.7475451452569 27.31731709274014 23.26613275817946 90-91 22.830222014808594 26.776807157372463 27.24229374155824 23.150677086260703 92-93 22.864833557138354 26.86724312146585 27.171397376176117 23.096525945219682 94-95 22.59919854373614 26.756254702569425 27.292693701743993 23.351853051950442 96-97 22.73670926570303 26.691815418998864 27.208029853577493 23.363445461720612 98-99 22.659067999460845 26.81046446069818 27.062424751926674 23.468042787914303 100 22.99777815862042 26.71393647646215 26.716797869662322 23.571487495255113 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2803.0 1 1965.5 2 2530.5 3 3427.5 4 2213.0 5 1667.5 6 2493.0 7 4443.5 8 6983.0 9 8115.0 10 6832.5 11 5363.5 12 5388.0 13 5992.0 14 6509.5 15 7016.0 16 7622.5 17 8273.0 18 8961.0 19 9671.5 20 10386.0 21 11411.5 22 13071.0 23 15214.0 24 17924.0 25 21816.0 26 26931.5 27 33067.5 28 40126.0 29 49479.0 30 61848.0 31 74177.5 32 89399.0 33 106401.5 34 121907.5 35 136779.5 36 154454.0 37 174886.5 38 185673.0 39 188182.0 40 189402.0 41 190475.5 42 195191.0 43 204994.0 44 215730.0 45 230616.5 46 246338.0 47 256053.5 48 263406.5 49 266749.0 50 263960.0 51 261816.5 52 256815.0 53 247480.0 54 238287.5 55 221331.0 56 200305.5 57 176334.0 58 153842.5 59 136263.5 60 115001.0 61 93149.0 62 75398.0 63 60109.5 64 45435.5 65 33093.5 66 24414.5 67 18533.5 68 14217.0 69 10640.0 70 8076.0 71 6180.5 72 4853.5 73 3835.0 74 2875.5 75 2126.0 76 1570.5 77 1098.0 78 768.0 79 530.0 80 348.0 81 232.0 82 179.0 83 140.5 84 102.0 85 84.5 86 71.0 87 47.0 88 35.5 89 29.5 90 29.5 91 24.0 92 19.5 93 16.0 94 10.0 95 7.5 96 5.0 97 2.5 98 2.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02433615572950781 2 0.11043233689873469 3 0.016568650684049665 4 0.017830300231550692 5 0.02004958738739588 6 0.04541938371003706 7 0.04639222191533906 8 0.03876152224250151 9 0.03348691509812973 10-11 0.03719586075584361 12-13 0.03518178165892931 14-15 0.03102441839095905 16-17 0.036663839862319074 18-19 0.03719586075584361 20-21 0.048170691759406775 22-23 0.04847470369856365 24-25 0.06774146034263058 26-27 0.0590923206736175 28-29 0.05124121234489121 30-31 0.04839110041529551 32-33 0.044172934759493875 34-35 0.04541178341155814 36-37 0.04102641118922022 38-39 0.045890602215730214 40-41 0.05525416994176196 42-43 0.057070641278224275 44-45 0.06011836096827195 46-47 0.061311607829462676 48-49 0.06062758096635971 50-51 0.05377211173837219 52-53 0.06416171975905838 54-55 0.05011636817001077 56-57 0.054828553226942335 58-59 0.053316093829636875 60-61 0.04878631593619945 62-63 0.05536817441894577 64-65 0.05368090815662512 66-67 0.05195564040190987 68-69 0.05800547799113167 70-71 0.05835509172116207 72-73 0.06049077559373912 74-75 0.05122601174793336 76-77 0.051210811150975526 78-79 0.060855589920727364 80-81 0.06166122155949308 82-83 0.05206204458061477 84-85 0.057830671126116465 86-87 0.05563418486570805 88-89 0.06409331707274808 90-91 0.06993034630456008 92-93 0.07427771703450338 94-95 0.07835907731768442 96-97 0.07403450748317789 98-99 0.0825544420780493 100 0.12864265205423148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6578689.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.58449283824073 #Duplication Level Percentage of deduplicated Percentage of total 1 73.46383273127827 42.303775497840086 2 15.068261663613129 17.353964117061352 3 5.225277501779776 9.02684864637174 4 2.281195209219775 5.254458767517808 5 1.1786144038125703 3.3934956347696166 6 0.7128105859054042 2.462810164745516 7 0.45765852774917026 1.8447823949472326 8 0.3019516946350022 1.391018815776315 9 0.2264527593112563 1.1736150567083004 >10 1.0154323330714552 9.862648740716747 >50 0.04626901898268617 1.8227322518412141 >100 0.0207246023562444 2.068068742870279 >500 0.0010701833841064291 0.4232905272151877 >1k 3.452191547151167E-4 0.49916846825055294 >5k 0.0 0.0 >10k+ 1.0356574641414981E-4 1.1193221733678904 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24661 0.3748619215773842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19698 0.2994213588756058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11947 0.18160153185535902 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 1.520059695784373E-5 0.0 0.0 0.0 9 0.0 1.520059695784373E-5 0.0 0.0 0.0 10-11 0.0 3.040119391568746E-5 0.0 2.2800895436765594E-5 0.0 12-13 0.0 3.040119391568746E-5 0.0 6.080238783137492E-5 0.0 14-15 0.0 3.040119391568746E-5 0.0 7.600298478921865E-5 0.0 16-17 0.0 3.040119391568746E-5 0.0 9.880388022598423E-5 3.040119391568746E-5 18-19 0.0 3.040119391568746E-5 0.0 1.2160477566274983E-4 4.560179087353119E-5 20-21 0.0 3.040119391568746E-5 0.0 1.3680537262059355E-4 4.560179087353119E-5 22-23 0.0 3.040119391568746E-5 0.0 3.4961373003040573E-4 9.880388022598423E-5 24-25 0.0 3.800149239460932E-5 0.0 8.9683522051278E-4 2.0520805893089035E-4 26-27 0.0 4.560179087353119E-5 0.0 0.0019152752166883098 2.2800895436765592E-4 28-29 0.0 4.560179087353119E-5 0.0 0.005183403562624711 2.2800895436765592E-4 30-31 0.0 4.560179087353119E-5 0.0 0.01577821964224179 2.3560925284657778E-4 32-33 0.0 6.080238783137492E-5 0.0 0.04048678999721678 2.4320955132549967E-4 34-35 0.0 6.080238783137492E-5 0.0 0.07788025851351235 2.888113421990308E-4 36-37 0.0 6.840268631029677E-5 0.0 0.13767180664719064 3.040119391568746E-4 38-39 0.0 7.600298478921865E-5 0.0 0.22297755677460965 3.040119391568746E-4 40-41 0.0 8.360328326814052E-5 0.0 0.31323110121180675 3.040119391568746E-4 42-43 0.0 9.120358174706237E-5 0.0 0.4031274316204946 3.040119391568746E-4 44-45 0.0 9.120358174706237E-5 0.0 0.5166682905971083 3.040119391568746E-4 46-47 0.0 9.120358174706237E-5 0.0 0.6517939972538601 3.040119391568746E-4 48-49 0.0 9.120358174706237E-5 0.0 0.7959488585035712 3.192125361147183E-4 50-51 0.0 9.880388022598423E-5 0.0 0.9443142851106049 3.192125361147183E-4 52-53 0.0 1.064041787049061E-4 0.0 1.0902856176967781 3.4961373003040573E-4 54-55 7.600298478921865E-6 1.064041787049061E-4 0.0 1.255364100658961 4.104161178617807E-4 56-57 1.520059695784373E-5 1.1400447718382798E-4 0.0 1.4454931674076703 4.4081731177746813E-4 58-59 1.520059695784373E-5 1.3680537262059355E-4 0.0 1.6621092743554224 4.4081731177746813E-4 60-61 1.520059695784373E-5 1.3680537262059355E-4 0.0 1.881301882487529 4.5601790873531185E-4 62-63 1.520059695784373E-5 1.3680537262059355E-4 0.0 2.0965727366045117 5.01619699608843E-4 64-65 1.520059695784373E-5 1.3680537262059355E-4 0.0 2.3214275671034152 5.01619699608843E-4 66-67 1.520059695784373E-5 1.3680537262059355E-4 0.0 2.5758475586853247 5.092199980877649E-4 68-69 1.520059695784373E-5 1.3680537262059355E-4 0.0 2.8641952826771413 5.168202965666868E-4 70-71 1.520059695784373E-5 1.3680537262059355E-4 0.0 3.1695980764556584 5.472214904823742E-4 72-73 1.520059695784373E-5 1.3680537262059355E-4 0.0 3.469452652344563 6.840268631029678E-4 74-75 1.520059695784373E-5 1.3680537262059355E-4 0.0 3.765750288545332 6.840268631029678E-4 76-77 1.520059695784373E-5 1.3680537262059355E-4 0.0 4.09377157059712 6.840268631029678E-4 78-79 1.520059695784373E-5 1.3680537262059355E-4 0.0 4.450233169557034 6.840268631029678E-4 80-81 1.520059695784373E-5 1.3680537262059355E-4 0.0 4.826759556501303 7.29628653976499E-4 82-83 1.520059695784373E-5 1.3680537262059355E-4 0.0 5.205239220154654 7.980313402867957E-4 84-85 2.2800895436765594E-5 1.3680537262059355E-4 0.0 5.580929574266241 8.056316387657176E-4 86-87 3.040119391568746E-5 1.3680537262059355E-4 0.0 5.966606720579131 8.056316387657176E-4 88 3.040119391568746E-5 1.3680537262059355E-4 0.0 6.276463289266296 8.056316387657176E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11395 0.0 52.13272 1 GTATCAA 20075 0.0 40.556725 1 CAACGCA 24965 0.0 31.978413 5 ATCAACG 25195 0.0 31.7061 3 TATCAAC 25620 0.0 31.375517 2 TCAACGC 26250 0.0 30.50319 4 AACGCAG 26640 0.0 30.144829 6 GTGGTAT 5065 0.0 29.344652 1 ACGCAGA 29785 0.0 26.678265 7 GTACATG 26060 0.0 26.676043 1 CGCAGAG 30405 0.0 26.04074 8 TACATGG 26360 0.0 25.980286 2 TGGTATC 5555 0.0 25.063147 2 ACATGGG 26960 0.0 24.384066 3 GCAGAGT 33025 0.0 23.591557 9 GTACTAG 2450 0.0 22.845581 1 CATGGGG 18855 0.0 22.828318 4 TACCTGG 4775 0.0 22.360447 2 GAGTACT 19695 0.0 21.818228 12-13 ATGGGGG 12225 0.0 21.639866 5 >>END_MODULE