FastQCFastQC Report
Mon 6 Jun 2016
ERR1211288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6578689
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222000.33745325246413077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188160.2860144323587876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116780.17751257127369904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112500.052.604761
GTATCAA199800.040.5422441
CAACGCA250300.031.6752415
ATCAACG252700.031.4191823
TATCAAC262500.030.6724242
TCAACGC262950.030.2122824
AACGCAG268550.029.8196436
GTACTAG24450.027.6405141
ACGCAGA301800.026.2543457
GTGGTAT50700.025.8260941
GTACATG261750.025.7292841
CGCAGAG310800.025.478998
TACATGG265450.024.887432
ACATGGG265950.023.9254133
CATGGGG184600.022.7505074
GCAGAGT343800.022.7457759
TGGTATC57150.022.4133382
ATGGGGG120900.021.535795
GAGTACT204600.021.35560612-13
TACCTGG47000.021.1640192
Produced by FastQC (version 0.11.5)