##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211288_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6578689 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11766432491337 33.0 31.0 34.0 30.0 34.0 2 32.24887253372214 34.0 31.0 34.0 30.0 34.0 3 32.3106153824873 34.0 31.0 34.0 30.0 34.0 4 35.94877809241324 37.0 35.0 37.0 35.0 37.0 5 35.85557532815429 37.0 35.0 37.0 35.0 37.0 6 35.873146154195766 37.0 35.0 37.0 35.0 37.0 7 35.787502342792 37.0 35.0 37.0 33.0 37.0 8 35.77315343528171 37.0 35.0 37.0 33.0 37.0 9 37.3914337643868 39.0 37.0 39.0 34.0 39.0 10-11 37.34151302789963 39.0 37.0 39.0 34.0 39.0 12-13 37.269716656312525 39.0 37.0 39.0 34.0 39.0 14-15 38.50444138642213 40.0 38.0 41.0 34.0 41.0 16-17 38.4887020803081 40.0 38.0 41.0 34.0 41.0 18-19 38.49722520702833 40.0 38.0 41.0 34.0 41.0 20-21 38.46404823210217 40.0 38.0 41.0 34.0 41.0 22-23 38.35255063736863 40.0 38.0 41.0 34.0 41.0 24-25 38.2593832144976 40.0 38.0 41.0 34.0 41.0 26-27 38.15894739818222 40.0 38.0 41.0 33.0 41.0 28-29 38.0546079165621 40.0 37.0 41.0 33.0 41.0 30-31 38.05389607260656 40.0 37.0 41.0 33.0 41.0 32-33 37.98411948034023 40.0 37.0 41.0 33.0 41.0 34-35 37.847448176984805 40.0 37.0 41.0 33.0 41.0 36-37 37.69650868128893 40.0 37.0 41.0 33.0 41.0 38-39 37.580628906458415 40.0 37.0 41.0 32.5 41.0 40-41 37.46915099649793 40.0 36.0 41.0 32.0 41.0 42-43 37.35669827225455 40.0 36.0 41.0 32.0 41.0 44-45 37.19112508586437 40.0 36.0 41.0 31.0 41.0 46-47 36.94382573792438 39.0 36.0 41.0 31.0 41.0 48-49 35.39832107886541 39.0 35.0 40.5 24.0 41.0 50-51 36.11967483187 39.0 35.0 40.0 28.5 41.0 52-53 36.20536918525865 39.0 35.0 40.0 29.0 41.0 54-55 36.03525368048254 38.0 35.0 40.0 29.0 41.0 56-57 35.818093164154746 38.0 35.0 40.0 28.0 41.0 58-59 35.57671878089997 38.0 34.0 40.0 28.0 41.0 60-61 35.32143053121982 37.5 34.0 40.0 28.0 41.0 62-63 35.00469516038834 37.0 34.0 40.0 27.0 41.0 64-65 34.66953446195739 36.5 33.5 39.0 26.5 41.0 66-67 34.33232730715801 36.0 33.0 39.0 26.0 40.5 68-69 33.97140524502679 35.5 33.0 39.0 26.0 40.0 70-71 33.61017043061315 35.0 32.5 38.0 26.0 40.0 72-73 33.23801284724054 35.0 32.0 37.0 25.5 39.5 74-75 32.87891827687857 35.0 32.0 37.0 25.0 39.0 76-77 31.955559306725092 34.0 30.5 36.0 24.5 38.0 78-79 32.39249096590521 34.5 31.5 36.0 25.0 38.0 80-81 32.267661459600845 35.0 32.0 35.5 25.0 37.0 82-83 32.004192172635 34.0 31.0 35.0 24.5 37.0 84-85 31.7283010338382 34.0 31.0 35.0 24.0 36.5 86-87 31.469114971083144 34.0 31.0 35.0 23.5 36.0 88-89 31.240133178510185 34.0 31.0 35.0 23.0 36.0 90-91 31.018700686413357 34.0 30.5 35.0 22.5 35.0 92-93 30.8295906676847 34.0 30.0 35.0 20.5 35.0 94-95 30.635501693422505 34.0 30.0 35.0 20.0 35.0 96-97 30.445227536975832 34.0 29.5 35.0 20.0 35.0 98-99 30.227189338179688 34.0 29.0 35.0 19.0 35.0 100 27.78655534560153 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26445588334443215 1101 2 -0.25798298399967834 1101 3 -0.25742141330680823 1101 4 -0.13893131438684492 1101 5 -0.16374047819062554 1101 6 -0.19627098494833461 1101 7 -0.20644959477248648 1101 8 -0.23316993927158336 1101 9 -0.2856034005664725 1101 10-11 -0.3065633187677861 1101 12-13 -0.3342880432166382 1101 14-15 -0.4581171610339254 1101 16-17 -0.4970938264607483 1101 18-19 -0.5481877389400154 1101 20-21 -0.5241710778000268 1101 22-23 -0.5763394831085051 1101 24-25 -0.47601964521603435 1101 26-27 -0.5504939197306271 1101 28-29 -0.49289067197980074 1101 30-31 -0.38178221171074966 1101 32-33 -0.4407592835217997 1101 34-35 -0.5016770359578473 1101 36-37 -0.5441481455865045 1101 38-39 -0.5067756360454467 1101 40-41 -0.5188094911331831 1101 42-43 -0.5454578335333906 1101 44-45 -0.49134589195352874 1101 46-47 -0.4642106913267412 1101 48-49 0.72247423373868 1101 50-51 -0.11516490599525753 1101 52-53 -0.47626451240332557 1101 54-55 -0.42171859776555465 1101 56-57 -0.5154437521908264 1101 58-59 -0.6425701005455338 1101 60-61 -0.6138183648084379 1101 62-63 -0.6515842368211651 1101 64-65 -0.741912790487973 1101 66-67 -0.7293150215836164 1101 68-69 -0.746347818277215 1101 70-71 -0.7353742470903128 1101 72-73 -0.8100281437661323 1101 74-75 -0.758994430032871 1101 76-77 -0.7094388534185931 1101 78-79 -0.5829700759344973 1101 80-81 -0.4998715723688001 1101 82-83 -0.5321967764015021 1101 84-85 -0.5617651143118785 1101 86-87 -0.5787335278916892 1101 88-89 -0.6574490672536086 1101 90-91 -0.6639112264366709 1101 92-93 -0.7297419671656833 1101 94-95 -0.6436665965875363 1101 96-97 -0.7774903444147263 1101 98-99 -0.9176816263144332 1101 100 -0.8586233533499694 1102 1 -0.21023398961043682 1102 2 -0.225495860459354 1102 3 -0.2703959160829541 1102 4 -0.12633876527645072 1102 5 -0.18479059303219714 1102 6 -0.2291750329418818 1102 7 -0.1588316163360659 1102 8 -0.16936863284017534 1102 9 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64-65 0.9977349358061574 2206 66-67 1.089355178886855 2206 68-69 1.1027311869396428 2206 70-71 1.0699378464051605 2206 72-73 1.112472932691837 2206 74-75 1.1585316202501872 2206 76-77 1.0826867318346096 2206 78-79 0.9753917589844434 2206 80-81 0.922448137916696 2206 82-83 0.9849849546335712 2206 84-85 1.099641419674736 2206 86-87 1.030525574410344 2206 88-89 1.0380685277353088 2206 90-91 1.0066902302942502 2206 92-93 1.082241480309964 2206 94-95 1.0266100417815025 2206 96-97 1.1271848145611223 2206 98-99 1.2203619849641285 2206 100 1.340307803196481 2207 1 0.5085525924704939 2207 2 0.5165394148298006 2207 3 0.489284188669906 2207 4 0.34836111738276543 2207 5 0.3765054112160655 2207 6 0.4015085946303216 2207 7 0.4505576071316142 2207 8 0.43125917789897983 2207 9 0.5873886128742072 2207 10-11 0.5946370498663143 2207 12-13 0.6378236178369221 2207 14-15 0.7901892914475042 2207 16-17 0.7357895353099266 2207 18-19 0.7144794426245937 2207 20-21 0.7388307361382189 2207 22-23 0.7342701324565937 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0.9064923478986451 2207 92-93 0.8670714095134393 2207 94-95 0.8843371328325738 2207 96-97 0.9670074531657669 2207 98-99 1.0144795449450292 2207 100 1.322770471259588 2208 1 0.5478927659893742 2208 2 0.5496275886983994 2208 3 0.5051685493695288 2208 4 0.33363460307673876 2208 5 0.4080172373401183 2208 6 0.41424776914384154 2208 7 0.48350167215123463 2208 8 0.4581134001487186 2208 9 0.5859733204008108 2208 10-11 0.6141623576311517 2208 12-13 0.6317329213198732 2208 14-15 0.7806048687603351 2208 16-17 0.7716458971418589 2208 18-19 0.7236240937223641 2208 20-21 0.7432390917415006 2208 22-23 0.7797462510921136 2208 24-25 0.7554623209643481 2208 26-27 0.7600110774075759 2208 28-29 0.8512679540193417 2208 30-31 0.834129223367114 2208 32-33 0.7941327567693932 2208 34-35 0.8395910331985732 2208 36-37 0.8645053314937314 2208 38-39 0.8680983915854767 2208 40-41 0.8889837828181371 2208 42-43 0.8963863862096417 2208 44-45 0.888905209108728 2208 46-47 0.911442477290116 2208 48-49 2.1667961892750753 2208 50-51 1.2935424813451135 2208 52-53 1.0203565791252984 2208 54-55 1.0415512907806388 2208 56-57 1.0337511197168183 2208 58-59 0.9932562995573235 2208 60-61 1.0006858696935197 2208 62-63 1.0360607727841966 2208 64-65 1.0351059975356236 2208 66-67 0.9916253694313824 2208 68-69 0.9809051775030184 2208 70-71 0.9985819470625401 2208 72-73 0.9709140407347192 2208 74-75 0.9636600149360959 2208 76-77 0.9669308955478009 2208 78-79 0.8732979435277883 2208 80-81 0.8860076024496166 2208 82-83 0.8756228511785444 2208 84-85 0.9533290614597227 2208 86-87 0.9976609197315263 2208 88-89 0.9800997403638725 2208 90-91 0.9823321312458866 2208 92-93 0.9787954437665825 2208 94-95 1.012387585213915 2208 96-97 1.0448791822997379 2208 98-99 1.1373250864405016 2208 100 1.4574246304018033 2209 1 0.3071215365571547 2209 2 0.3822522270380375 2209 3 0.42757357246100725 2209 4 0.24600160640395785 2209 5 0.3650784266401672 2209 6 0.39512213421306086 2209 7 0.4285319458226482 2209 8 0.4978495854477174 2209 9 0.5955854930267961 2209 10-11 0.6468997591759305 2209 12-13 0.6690554835204452 2209 14-15 0.8211877179404894 2209 16-17 0.776100428633022 2209 18-19 0.7354377535552103 2209 20-21 0.8033167997143309 2209 22-23 0.813797749451787 2209 24-25 0.848545760007589 2209 26-27 0.8657057893342 2209 28-29 0.8346289992818043 2209 30-31 0.828382596327792 2209 32-33 0.8789187286614748 2209 34-35 0.9268488626241265 2209 36-37 0.9153072588691344 2209 38-39 0.8835881605489107 2209 40-41 0.9054481416611253 2209 42-43 0.9562759108498042 2209 44-45 0.978145533474347 2209 46-47 1.0160185796538244 2209 48-49 2.251255400287093 2209 50-51 1.3758486473209501 2209 52-53 1.1408957372626745 2209 54-55 1.114488032036789 2209 56-57 1.1254132840900937 2209 58-59 1.1152601620417073 2209 60-61 1.0557412380563136 2209 62-63 1.0568481030620518 2209 64-65 1.056519531483275 2209 66-67 1.1132762628546615 2209 68-69 1.179884508485486 2209 70-71 1.1538393547225567 2209 72-73 1.164083909489733 2209 74-75 1.1229952293866887 2209 76-77 1.1108078302360873 2209 78-79 1.0747877103430028 2209 80-81 1.016468633712897 2209 82-83 0.9994432413582182 2209 84-85 1.041686488775877 2209 86-87 1.040878657800814 2209 88-89 1.095029971822921 2209 90-91 1.1210964656350662 2209 92-93 1.073728868900158 2209 94-95 1.0580027094825866 2209 96-97 1.1941080996619782 2209 98-99 1.2130441197023352 2209 100 1.4474510211808216 2210 1 0.5226301449849515 2210 2 0.47683690202952533 2210 3 0.45744067194878113 2210 4 0.3155657311192641 2210 5 0.39119760951580673 2210 6 0.4216326013282554 2210 7 0.4463331099019854 2210 8 0.44592309522546003 2210 9 0.6441394691343731 2210 10-11 0.5734720080131552 2210 12-13 0.605102325933423 2210 14-15 0.7463948350552414 2210 16-17 0.7804284651435793 2210 18-19 0.7419778025966366 2210 20-21 0.7387151958329099 2210 22-23 0.7814814584493988 2210 24-25 0.776399543265434 2210 26-27 0.77268040472552 2210 28-29 0.8188163689337173 2210 30-31 0.8329849855700928 2210 32-33 0.8207055119105959 2210 34-35 0.8605540206998228 2210 36-37 0.8648371767553016 2210 38-39 0.9457598578882767 2210 40-41 0.9494946699297557 2210 42-43 0.9075543910892563 2210 44-45 0.9666124217873104 2210 46-47 0.9642725479077967 2210 48-49 2.212102979194043 2210 50-51 1.3316913831691863 2210 52-53 1.0995107969937337 2210 54-55 1.0994306570618804 2210 56-57 1.0590841480743123 2210 58-59 1.0338253949884688 2210 60-61 1.0054817199188406 2210 62-63 1.0088297430700806 2210 64-65 1.0331222348699214 2210 66-67 1.0591405524957551 2210 68-69 1.070856959771298 2210 70-71 1.1060025876169632 2210 72-73 1.1095485377086263 2210 74-75 1.072944268192245 2210 76-77 1.1006340909584402 2210 78-79 0.9371476325050168 2210 80-81 0.9446897913348238 2210 82-83 0.9273860321817118 2210 84-85 0.9825817129091412 2210 86-87 0.99193610258558 2210 88-89 0.9619905379808493 2210 90-91 1.0803803910432386 2210 92-93 1.0858945451394995 2210 94-95 1.1521187858196598 2210 96-97 1.1745978157976502 2210 98-99 1.2029216034061 2210 100 1.3942594018539616 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1.0817583772851052 2211 62-63 1.0633904125619509 2211 64-65 1.1399283476366264 2211 66-67 1.130869297403855 2211 68-69 1.092347239698782 2211 70-71 1.0355178802287455 2211 72-73 1.1041098916825192 2211 74-75 1.0950219278113096 2211 76-77 0.8789265821050982 2211 78-79 0.7866544118716519 2211 80-81 0.8043080813789487 2211 82-83 0.8678280022236429 2211 84-85 0.9903830796486197 2211 86-87 0.9548679957687334 2211 88-89 0.992937912436151 2211 90-91 0.9909409989317233 2211 92-93 0.9922616824731172 2211 94-95 0.988725979554598 2211 96-97 1.077267056116721 2211 98-99 1.1811832329695626 2211 100 1.2444054104253865 2212 1 0.5180144663548205 2212 2 0.5152545146886283 2212 3 0.4983411803002724 2212 4 0.3138816734806156 2212 5 0.3441609546979336 2212 6 0.31936680612128754 2212 7 0.2478681020664908 2212 8 0.3691956557116427 2212 9 0.5470879077690824 2212 10-11 0.5547757575831653 2212 12-13 0.5551466633565028 2212 14-15 0.7863485865363415 2212 16-17 0.6998272343978726 2212 18-19 0.7457357814216934 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1.0946338054483995 2212 88-89 1.125310111424259 2212 90-91 1.177961325951312 2212 92-93 1.1921591172451471 2212 94-95 1.2326149062951401 2212 96-97 1.269138961690711 2212 98-99 1.2817233463254887 2212 100 1.5110655785156588 2213 1 0.40509809443621947 2213 2 0.41889225548167985 2213 3 0.43195398408032304 2213 4 0.2916655168133957 2213 5 0.336624047691231 2213 6 0.36851326880334057 2213 7 0.4208095095633624 2213 8 0.44504344970719956 2213 9 0.5396747380844289 2213 10-11 0.5066867485962803 2213 12-13 0.4959525329940746 2213 14-15 0.6546434756238853 2213 16-17 0.7153931923802546 2213 18-19 0.6733254890846894 2213 20-21 0.5144711769495913 2213 22-23 0.6801018167444042 2213 24-25 0.6805267997689768 2213 26-27 0.7901027855798475 2213 28-29 0.8430145522910735 2213 30-31 0.8000298525250358 2213 32-33 0.8126849049902773 2213 34-35 0.778993629915739 2213 36-37 0.7685700646984941 2213 38-39 0.7400077663087501 2213 40-41 0.7277426241094815 2213 42-43 0.8479981033855282 2213 44-45 0.8479741411152588 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0.3594606117172745 2311 7 0.3715169155931122 2311 8 0.21642431536417206 2311 9 0.4175637420458642 2311 10-11 0.37474075605094725 2311 12-13 0.48613138093242725 2311 14-15 0.40691610542656065 2311 16-17 0.4517710280057017 2311 18-19 0.5946540448548205 2311 20-21 0.6779942080200243 2311 22-23 0.701753688520462 2311 24-25 0.6426273702768768 2311 26-27 0.6825857012691756 2311 28-29 0.7640952526851734 2311 30-31 0.6158333494425676 2311 32-33 0.6125343464283972 2311 34-35 0.7405874318080805 2311 36-37 0.8204924840019174 2311 38-39 0.8365354308539992 2311 40-41 0.7489500670813385 2311 42-43 0.8330146010101984 2311 44-45 0.8888228885795613 2311 46-47 0.8975882926918857 2311 48-49 -5.825318532350895 2311 50-51 -0.9518370296515499 2311 52-53 0.4100221671167432 2311 54-55 0.7976004133195502 2311 56-57 0.9219690447549667 2311 58-59 0.9684534845794772 2311 60-61 0.7955164332219127 2311 62-63 0.6690625148541969 2311 64-65 0.7823368330090403 2311 66-67 0.9377674148127326 2311 68-69 1.0149305997804277 2311 70-71 1.003980172027731 2311 72-73 0.5102686514455286 2311 74-75 0.7798244769715339 2311 76-77 0.8011499384910543 2311 78-79 0.6998212703735618 2311 80-81 0.7574002652623832 2311 82-83 0.7554633861218285 2311 84-85 0.8140063140450842 2311 86-87 0.8489159025113757 2311 88-89 0.8784324779300512 2311 90-91 0.9823285815275788 2311 92-93 0.9760334518666731 2311 94-95 0.9546661464669768 2311 96-97 0.9191874995605325 2311 98-99 0.9892687328692382 2311 100 1.2037627483162723 2312 1 0.026978096848445432 2312 2 0.17739562491876626 2312 3 0.1151079982224914 2312 4 -0.04661452719923176 2312 5 0.13055713717791662 2312 6 0.06361621484209934 2312 7 0.22348592525644762 2312 8 0.3028584058114987 2312 9 0.2678398733506313 2312 10-11 0.1929028377151667 2312 12-13 0.220935517673432 2312 14-15 0.27018966676831724 2312 16-17 0.36957730194355776 2312 18-19 0.23510100272768852 2312 20-21 0.2766689152934063 2312 22-23 0.5542032654764384 2312 24-25 0.6604224230034745 2312 26-27 0.5808457822394573 2312 28-29 0.6935622808077753 2312 30-31 0.7436524695569773 2312 32-33 0.7457601146520219 2312 34-35 0.3863457539153501 2312 36-37 0.6428027116338697 2312 38-39 0.739412590900784 2312 40-41 0.5183651821838922 2312 42-43 0.5469480579903134 2312 44-45 0.6160358886366168 2312 46-47 0.7610097236917639 2312 48-49 -4.811926322345705 2312 50-51 -0.8944539238182045 2312 52-53 0.49563506974865135 2312 54-55 0.7193577343230331 2312 56-57 0.9095640666559817 2312 58-59 0.882859702396722 2312 60-61 0.8885328338008165 2312 62-63 0.6882549613198492 2312 64-65 0.8701911398438185 2312 66-67 0.8853024760039929 2312 68-69 0.7646247362681962 2312 70-71 0.7876090488033967 2312 72-73 0.8910464382064234 2312 74-75 0.561109907109234 2312 76-77 0.7256607497133025 2312 78-79 0.38109402570923123 2312 80-81 0.6703262947030026 2312 82-83 0.6401139734670984 2312 84-85 0.7171637860619136 2312 86-87 0.8417218653553356 2312 88-89 0.8659444595930417 2312 90-91 0.6895246271494493 2312 92-93 0.8184156120473567 2312 94-95 0.9691736098823327 2312 96-97 1.032369006218449 2312 98-99 1.0601909086751995 2312 100 0.6158485058775653 2313 1 0.04379599080238705 2313 2 0.1989579832884587 2313 3 0.24247811569747313 2313 4 0.05564932407401102 2313 5 0.10145757366577612 2313 6 0.23873210744778106 2313 7 0.1602151269633083 2313 8 0.23552919041654974 2313 9 0.40920344053660074 2313 10-11 0.170253130880063 2313 12-13 -0.03414927892107045 2313 14-15 0.06645609885459436 2313 16-17 0.07470680329233659 2313 18-19 0.3689043648663599 2313 20-21 0.3777883902216601 2313 22-23 0.44118590131726165 2313 24-25 0.24580686955900433 2313 26-27 0.3530977183036299 2313 28-29 0.3475178677118649 2313 30-31 0.5035700162962442 2313 32-33 0.38558029384574866 2313 34-35 0.26310634769599517 2313 36-37 -0.369496478748502 2313 38-39 0.13241293513036112 2313 40-41 0.3368160623916552 2313 42-43 0.4802031293120379 2313 44-45 0.4661686833760257 2313 46-47 0.4446087362198696 2313 48-49 -5.179875147487497 2313 50-51 -1.2317449418939646 2313 52-53 0.1224250688027908 2313 54-55 0.309369496082887 2313 56-57 0.22894659156595765 2313 58-59 0.35044575821853385 2313 60-61 0.7581501482410502 2313 62-63 0.8141624526265474 2313 64-65 0.8352700836965781 2313 66-67 0.8343597140985182 2313 68-69 0.8700610102081114 2313 70-71 0.7916521209838763 2313 72-73 0.6489308615173641 2313 74-75 0.7402623704025331 2313 76-77 0.7893143059215397 2313 78-79 0.756138793381183 2313 80-81 -0.3006893073418766 2313 82-83 0.13223296693533726 2313 84-85 0.5767989757625465 2313 86-87 0.749496860851476 2313 88-89 0.6951203212698012 2313 90-91 0.7154151271890505 2313 92-93 0.7426823170565164 2313 94-95 0.7016317257622902 2313 96-97 0.6728910320528669 2313 98-99 0.902237436713726 2313 100 0.9256609119423516 2314 1 0.03937621388111978 2314 2 0.16313637672656967 2314 3 0.202311773351326 2314 4 0.052142420150318 2314 5 0.14508554488447345 2314 6 0.24592638957741997 2314 7 0.061778750580891995 2314 8 0.18485896665625745 2314 9 0.36609435895944387 2314 10-11 0.30772028734256907 2314 12-13 0.19508160853947487 2314 14-15 0.18003364159466173 2314 16-17 0.41541199524881733 2314 18-19 0.502120917313789 2314 20-21 0.5839740332317049 2314 22-23 0.27838165088711975 2314 24-25 0.4229108728193651 2314 26-27 0.5070459670424015 2314 28-29 0.653852862753169 2314 30-31 0.5053474619487943 2314 32-33 0.48573524440483595 2314 34-35 0.4251887357706394 2314 36-37 0.4745207814084722 2314 38-39 0.49455937592715316 2314 40-41 0.34345195222375224 2314 42-43 0.3289499979615371 2314 44-45 0.5901635911498389 2314 46-47 0.6867556012529192 2314 48-49 -6.299109985213647 2314 50-51 -1.4905846696038267 2314 52-53 -0.7632941994522398 2314 54-55 -0.2999635975453643 2314 56-57 0.008162874171297574 2314 58-59 0.5291703092583333 2314 60-61 0.6965657857290708 2314 62-63 0.596878754009559 2314 64-65 0.7553926532052486 2314 66-67 0.8545462320089356 2314 68-69 0.7658360690100423 2314 70-71 0.5613969115711228 2314 72-73 0.7480819369215368 2314 74-75 0.7852801326883565 2314 76-77 0.7584939581590824 2314 78-79 0.5273098176266373 2314 80-81 0.6498278783998899 2314 82-83 0.6739857790654291 2314 84-85 0.6622165146636227 2314 86-87 0.7218919806043367 2314 88-89 0.656761086341664 2314 90-91 0.7918160457648966 2314 92-93 0.8064364046773029 2314 94-95 0.9294295987389916 2314 96-97 1.0027680104891452 2314 98-99 1.0774449299781068 2314 100 -0.4156008640914983 2315 1 0.12204074946342303 2315 2 0.22008167133764545 2315 3 0.29482010498432487 2315 4 0.1398864179738908 2315 5 0.23773897162278246 2315 6 0.2956543362549695 2315 7 0.34550316056525077 2315 8 0.26872620463490193 2315 9 0.45765843885243385 2315 10-11 0.2577412581195162 2315 12-13 0.39891019227522406 2315 14-15 0.21241138763288347 2315 16-17 0.3602084086791777 2315 18-19 0.47617666557377447 2315 20-21 0.4062172262308721 2315 22-23 0.4303347670312192 2315 24-25 0.4461907023326148 2315 26-27 0.5746172110062133 2315 28-29 0.6072036528413562 2315 30-31 0.5274982701738722 2315 32-33 0.6550873040752023 2315 34-35 0.6615378935388989 2315 36-37 0.521905668846415 2315 38-39 0.630184191724382 2315 40-41 0.6426357922563994 2315 42-43 0.690465788389794 2315 44-45 0.6714548242315175 2315 46-47 0.5774020679603922 2315 48-49 -5.077362155972448 2315 50-51 -0.9647413043042263 2315 52-53 0.23078240622913881 2315 54-55 0.2790350195169182 2315 56-57 0.6296596208669811 2315 58-59 0.7307210131963089 2315 60-61 0.6264485760893095 2315 62-63 0.42330590039691884 2315 64-65 0.5658893772298299 2315 66-67 -0.21376465692529223 2315 68-69 0.5351733924587734 2315 70-71 0.4733808604250953 2315 72-73 0.5616307591573602 2315 74-75 0.776426497375823 2315 76-77 0.7125333832197249 2315 78-79 0.6972779647167968 2315 80-81 0.4907310954304265 2315 82-83 0.5242975068307452 2315 84-85 0.6425453591171326 2315 86-87 0.6458797155337201 2315 88-89 0.5532141911502038 2315 90-91 0.7310200630856265 2315 92-93 0.7880370984132732 2315 94-95 0.2847397395265858 2315 96-97 0.4365972743581956 2315 98-99 0.4145087944955499 2315 100 0.4775173204059975 2316 1 0.24450363573959777 2316 2 0.2962573518144467 2316 3 0.21470708589667709 2316 4 -0.005478011025850549 2316 5 0.14552965881981095 2316 6 0.10963469255905522 2316 7 0.23719960831652998 2316 8 0.29625353693835876 2316 9 0.4703652062591672 2316 10-11 0.39587355157308224 2316 12-13 0.33789101966347346 2316 14-15 0.4622973510034285 2316 16-17 0.42417263677436523 2316 18-19 0.12291075038058352 2316 20-21 0.47211026134565515 2316 22-23 0.6061246038784915 2316 24-25 0.6479376537328321 2316 26-27 0.6551771776226261 2316 28-29 0.6558804716288122 2316 30-31 0.5913831334344124 2316 32-33 0.5571124606550129 2316 34-35 0.5384793256142189 2316 36-37 0.4272390268474311 2316 38-39 0.48325848700815754 2316 40-41 0.07248322265499496 2316 42-43 0.07098421394770327 2316 44-45 0.34122162728989736 2316 46-47 0.3604595087091411 2316 48-49 -4.952179803573397 2316 50-51 -1.0654899170041787 2316 52-53 0.28342795496339335 2316 54-55 0.5295152769789766 2316 56-57 0.4275142093466968 2316 58-59 0.311599783882464 2316 60-61 0.427598920803014 2316 62-63 0.31063806167284014 2316 64-65 0.3645722173294743 2316 66-67 0.45959376035524 2316 68-69 0.13909479129438296 2316 70-71 -0.2028437614142291 2316 72-73 -0.0704908857383657 2316 74-75 0.2043522897993597 2316 76-77 0.19948665578148805 2316 78-79 0.2712653136229761 2316 80-81 0.23177436356913717 2316 82-83 0.15601550968053957 2316 84-85 0.25866702422665355 2316 86-87 0.2879561055146631 2316 88-89 0.12016854463600524 2316 90-91 0.15670346917148237 2316 92-93 0.24810968306195846 2316 94-95 0.5019707307664802 2316 96-97 0.6048142105021377 2316 98-99 0.5896055380534335 2316 100 -0.09258151133952452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 3.0 11 12.0 12 29.0 13 134.0 14 541.0 15 1640.0 16 4041.0 17 8100.0 18 13987.0 19 20489.0 20 29130.0 21 40673.0 22 50984.0 23 54669.0 24 56961.0 25 61953.0 26 71742.0 27 87573.0 28 107596.0 29 132348.0 30 161359.0 31 200792.0 32 252227.0 33 328227.0 34 453359.0 35 642686.0 36 995804.0 37 1460311.0 38 1179482.0 39 161818.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.544602231042866 19.755684612981042 14.201766983515402 23.497946172460694 2 15.641972266587704 20.376486475845866 40.02063976585768 23.960901491708753 3 18.66056251042191 25.496501049525538 30.11771193856768 25.725224501484874 4 12.875253807974834 16.727661667412917 37.7479412191753 32.649143305436944 5 13.418278787466068 37.55358429757581 34.585256282707114 14.44288063225101 6 31.29286000274219 36.92608219939362 17.5340333920786 14.247024405785593 7 27.721777917082846 32.354754071747145 21.83864348615461 18.084824525015403 8 26.14706547183008 35.55932985093526 19.76657910254093 18.527025574693727 9 26.358654176510115 15.950109304072072 20.408119209848614 37.2831173095692 10-11 25.2252562429896 26.158043200252212 27.763516932657144 20.853183624101046 12-13 25.6010642247604 23.753597888210344 28.219870063708015 22.42546782332124 14-15 22.973722014054033 25.28293853411204 25.853653197833033 25.8896862540009 16-17 22.3033574304319 28.176937802854134 26.70447273320928 22.815232033504685 18-19 22.048189703493286 27.190583228037507 28.923132463732316 21.838094604736888 20-21 22.995689258777176 26.363369385635394 28.839488085989245 21.801453269598188 22-23 22.942823638480807 26.13450907223442 28.624639488383025 22.29802780090175 24-25 22.59757740430747 26.287834769473395 28.818673937620538 22.2959138885986 26-27 22.48560730820714 26.496803465008057 28.61932458852725 22.398264638257547 28-29 22.304924539962023 26.561519906661196 28.822444300391826 22.311111252984954 30-31 22.727283782543054 26.346907114005653 28.77078964313532 22.155019460315977 32-33 22.65840559603848 26.610667060702497 28.42336109522502 22.307566248034004 34-35 22.74045197801957 26.591785496225917 28.596350156777177 22.071412368977334 36-37 22.719681194604963 26.47362810051048 28.43891661385881 22.367774091025748 38-39 22.648801774883662 26.55559833716784 28.56601821483101 22.229581673117483 40-41 22.59465360008075 26.298642467904088 28.78395502376867 22.322748908246492 42-43 22.517836070049434 26.43103175962847 28.436957835991 22.614174334331096 44-45 22.464734706591344 26.724207060591247 28.366868961547958 22.444189271269448 46-47 22.52118731835573 26.570613182789575 28.048182312603544 22.86001718625115 48-49 22.86578571932405 26.634092091404188 27.907937364325484 22.592184824946273 50-51 22.658138649205213 26.734681001317362 27.853800853967332 22.753379495510096 52-53 22.5525757845157 26.93256529873934 27.693844520885385 22.82101439585957 54-55 22.517729067887863 26.86964154514742 27.738152662214375 22.87447672475034 56-57 22.721428695757066 26.820523956759875 27.537468292026286 22.920579055456773 58-59 22.425245975031764 26.779025889848334 27.81388253473712 22.981845600382783 60-61 22.564480961164087 26.963056806057867 27.67581081992136 22.79665141285669 62-63 22.569191846829916 26.921628368951733 27.527799303074445 22.981380481143905 64-65 22.326953282201906 26.77451934486864 27.639522980787785 23.259004392141673 66-67 22.475655245062324 26.676984285309395 27.54303932504315 23.304321144585128 68-69 22.2664934404996 26.747492704802827 27.6630881307258 23.32292572397177 70-71 22.3027373609824 26.50882513260316 27.7029719680308 23.485465538383643 72-73 22.431360171064767 26.727372217605232 27.46988897028169 23.371378641048306 74-75 22.46256616489358 26.86746559364726 27.363271888287038 23.30669635317212 76-77 22.304775886981876 26.77663754344861 27.31411685910734 23.60446971046217 78-79 22.278871026391606 26.655252113359495 27.38595125196256 23.67992560828634 80-81 22.14154719766355 26.942832654064492 27.315256875046124 23.600363273225838 82-83 22.403279354454817 26.639426382059057 27.5774967701761 23.379797493310033 84-85 22.439586649276094 26.733536294307246 27.215889796250686 23.61098726016597 86-87 22.028196899672913 26.89672940229524 27.432399949096702 23.642673748935145 88-89 22.11456541774062 26.53868125829366 27.429550143030767 23.917203180934948 90-91 22.285881961221346 26.495257194028582 27.40602949829186 23.812831346458214 92-93 22.271902669229014 26.56290073135206 27.30270544163816 23.862491157780767 94-95 21.87513538912488 26.46834018899524 27.49599780245873 24.16052661942115 96-97 21.911154616644726 26.356387997914716 27.521567123436768 24.210890262003794 98-99 21.746949647085295 26.45282618717953 27.376899831210576 24.4233243345246 100 21.82317728496643 26.3276650769679 27.327527009774794 24.52163062829088 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2622.0 1 1870.0 2 2196.5 3 2958.0 4 1970.0 5 1457.0 6 2241.0 7 4207.0 8 6521.0 9 7557.5 10 6343.5 11 4821.5 12 4782.0 13 5399.0 14 6087.0 15 6658.0 16 7278.0 17 8001.0 18 8796.5 19 9532.0 20 10317.5 21 11407.5 22 12994.5 23 15209.0 24 18043.5 25 21685.0 26 26740.0 27 32720.5 28 40036.5 29 49613.5 30 61649.5 31 74361.0 32 89460.5 33 106117.0 34 122058.5 35 138010.0 36 154977.0 37 174321.0 38 185474.5 39 187956.0 40 189343.5 41 190193.0 42 194556.5 43 205251.0 44 217476.0 45 232866.5 46 248244.5 47 258405.0 48 266457.5 49 269883.0 50 266915.5 51 263921.0 52 259452.5 53 250939.5 54 242309.0 55 225243.0 56 202770.0 57 177730.5 58 154193.5 59 135625.0 60 113400.0 61 91189.0 62 73272.0 63 57319.5 64 42249.5 65 30231.0 66 21984.5 67 16481.5 68 12367.0 69 8991.5 70 6810.5 71 5194.0 72 4011.5 73 3181.5 74 2386.5 75 1647.5 76 1122.5 77 724.0 78 464.5 79 287.0 80 163.5 81 104.0 82 67.5 83 43.5 84 28.0 85 18.5 86 9.5 87 7.5 88 7.5 89 3.5 90 2.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02954996048604821 2 0.0019152752166883098 3 3.0401193915687455E-5 4 0.020885620220077283 5 5.472214904823742E-4 6 4.560179087353119E-5 7 3.0401193915687455E-5 8 0.004134562372533494 9 0.010777223243111204 10-11 0.001064041787049061 12-13 5.3202089352453045E-5 14-15 1.1400447718382798E-4 16-17 4.1041611786178065E-4 18-19 2.2800895436765594E-5 20-21 9.120358174706237E-5 22-23 2.6601044676226527E-4 24-25 2.2800895436765594E-5 26-27 4.560179087353119E-5 28-29 0.0011324444733593576 30-31 0.002629703273706965 32-33 0.009226762353411144 34-35 0.01825591694637032 36-37 0.021052826786613563 38-39 0.020619609773315017 40-41 0.004271367745154088 42-43 0.0046513826691001806 44-45 0.008899949518817504 46-47 0.011499251598608779 48-49 1.1936648776070733 50-51 0.017541488889351663 52-53 0.0183547208265963 54-55 0.009880388022598423 56-57 0.007501494598695879 58-59 0.008079117283093941 60-61 0.00505419848848304 62-63 0.007775105343937066 64-65 0.019152752166883096 66-67 0.029078741980355052 68-69 0.02934475242711732 70-71 0.026357835124901023 72-73 0.023272113942458747 74-75 0.009363567726031736 76-77 0.014402565617556934 78-79 0.008162720566362082 80-81 0.0025765011843545123 82-83 0.014151755767752511 84-85 0.023750932746630826 86-87 0.022983302600259718 88-89 0.03240767271412283 90-91 0.020657611265709627 92-93 0.013254920547239733 94-95 0.006505855497957115 96-97 0.003275728644415323 98-99 0.014691376959755963 100 0.03315250196505717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6578689.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.30282994940229 #Duplication Level Percentage of deduplicated Percentage of total 1 73.08718826821767 41.881027208136274 2 15.284138017995119 17.51648723536738 3 5.32288020625378 9.150482978999994 4 2.2775520009375714 5.220407000425864 5 1.2059659072698787 3.455262965453125 6 0.7249901672520184 2.492639296141868 7 0.4668697745978897 1.872707151160906 8 0.30976372388051376 1.4200270395214951 9 0.2338210963476711 1.2058749470334067 >10 1.018289296318404 9.949260428409747 >50 0.045808526202836944 1.801296796744848 >100 0.021300366090964553 2.129429409788095 >500 9.78395088980317E-4 0.380319407450744 >1k 3.4942580508963157E-4 0.4696815632276645 >5k 0.0 0.0 >10k+ 1.0482774152627572E-4 1.0550965721383534 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22200 0.33745325246413077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18816 0.2860144323587876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11678 0.17751257127369904 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.520059695784373E-5 2 0.0 0.0 0.0 0.0 1.520059695784373E-5 3 0.0 0.0 0.0 0.0 1.520059695784373E-5 4 0.0 0.0 0.0 0.0 6.080238783137492E-5 5 0.0 0.0 0.0 0.0 6.080238783137492E-5 6 0.0 0.0 0.0 0.0 9.120358174706237E-5 7 0.0 0.0 0.0 0.0 9.120358174706237E-5 8 0.0 0.0 0.0 0.0 9.120358174706237E-5 9 0.0 0.0 0.0 0.0 9.120358174706237E-5 10-11 0.0 0.0 0.0 0.0 1.2160477566274983E-4 12-13 0.0 0.0 0.0 3.800149239460932E-5 1.3680537262059355E-4 14-15 0.0 0.0 0.0 6.080238783137492E-5 1.67206566536281E-4 16-17 0.0 0.0 0.0 8.360328326814052E-5 1.67206566536281E-4 18-19 0.0 0.0 0.0 1.064041787049061E-4 1.9760776045196847E-4 20-21 0.0 0.0 0.0 1.2160477566274983E-4 2.0520805893089035E-4 22-23 0.0 0.0 0.0 3.34413133072562E-4 2.736107452411871E-4 24-25 0.0 0.0 0.0 8.9683522051278E-4 3.34413133072562E-4 26-27 0.0 0.0 0.0 0.0019152752166883098 3.34413133072562E-4 28-29 0.0 0.0 0.0 0.005160602667187946 3.34413133072562E-4 30-31 0.0 0.0 0.0 0.015801020537678557 3.34413133072562E-4 32-33 0.0 0.0 0.0 0.04047918969873785 3.4961373003040573E-4 34-35 0.0 0.0 0.0 0.07765984985762361 3.572140285093276E-4 36-37 0.0 1.520059695784373E-5 0.0 0.13689657620234064 3.648143269882495E-4 38-39 0.0 5.320208935245305E-5 0.0 0.21878979231272372 3.800149239460932E-4 40-41 0.0 6.080238783137492E-5 0.0 0.3053875931815594 4.1041611786178065E-4 42-43 0.0 6.080238783137492E-5 0.0 0.3906857430104995 4.1041611786178065E-4 44-45 0.0 6.080238783137492E-5 0.0 0.4992104049910248 4.1041611786178065E-4 46-47 0.0 6.080238783137492E-5 0.0 0.6285598848037961 4.3321701329854625E-4 48-49 0.0 6.080238783137492E-5 0.0 0.767371736222825 4.7121850569315556E-4 50-51 0.0 6.840268631029677E-5 0.0 0.9153647482043915 4.7121850569315556E-4 52-53 0.0 7.600298478921865E-5 0.0 1.061214476014902 5.092199980877649E-4 54-55 0.0 7.600298478921865E-5 0.0 1.2261789544999013 5.472214904823742E-4 56-57 0.0 7.600298478921865E-5 0.0 1.416551230799936 5.472214904823742E-4 58-59 0.0 7.600298478921865E-5 0.0 1.6330457329720254 5.700223859191399E-4 60-61 0.0 7.600298478921865E-5 0.0 1.8516607184197338 5.928232813559054E-4 62-63 0.0 7.600298478921865E-5 0.0 2.066209544181219 6.232244752715928E-4 64-65 0.0 7.600298478921865E-5 0.0 2.290615957069866 6.232244752715928E-4 66-67 0.0 7.600298478921865E-5 0.0 2.5443443214901933 6.232244752715928E-4 68-69 0.0 7.600298478921865E-5 0.0 2.8319624168280337 6.232244752715928E-4 70-71 0.0 7.600298478921865E-5 0.0 3.1364987765799537 6.840268631029678E-4 72-73 0.0 7.600298478921865E-5 0.0 3.436581361423226 7.448292509343427E-4 74-75 0.0 7.600298478921865E-5 0.0 3.733015802996615 7.448292509343427E-4 76-77 0.0 7.600298478921865E-5 0.0 4.060968682362094 7.448292509343427E-4 78-79 0.0 7.600298478921865E-5 0.0 4.417779895052039 7.448292509343427E-4 80-81 0.0 7.600298478921865E-5 0.0 4.794230279011517 7.524295494132645E-4 82-83 0.0 7.600298478921865E-5 0.0 5.172937951619236 7.904310418078739E-4 84-85 0.0 7.600298478921865E-5 0.0 5.549479539160462 7.904310418078739E-4 86-87 0.0 7.600298478921865E-5 0.0 5.9354682977109885 8.056316387657176E-4 88 0.0 7.600298478921865E-5 0.0 6.2452260625179274 8.056316387657176E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11250 0.0 52.60476 1 GTATCAA 19980 0.0 40.542244 1 CAACGCA 25030 0.0 31.675241 5 ATCAACG 25270 0.0 31.419182 3 TATCAAC 26250 0.0 30.672424 2 TCAACGC 26295 0.0 30.212282 4 AACGCAG 26855 0.0 29.819643 6 GTACTAG 2445 0.0 27.640514 1 ACGCAGA 30180 0.0 26.254345 7 GTGGTAT 5070 0.0 25.826094 1 GTACATG 26175 0.0 25.729284 1 CGCAGAG 31080 0.0 25.47899 8 TACATGG 26545 0.0 24.88743 2 ACATGGG 26595 0.0 23.925413 3 CATGGGG 18460 0.0 22.750507 4 GCAGAGT 34380 0.0 22.745775 9 TGGTATC 5715 0.0 22.413338 2 ATGGGGG 12090 0.0 21.53579 5 GAGTACT 20460 0.0 21.355606 12-13 TACCTGG 4700 0.0 21.164019 2 >>END_MODULE