FastQCFastQC Report
Mon 6 Jun 2016
ERR1211287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12693919
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446080.35141235736575915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326930.2575485159468876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196310.15464885194241432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211950.053.594951
GTATCAA407150.043.519241
CAACGCA498400.034.714095
ATCAACG500250.034.623833
TATCAAC516400.034.082952
AACGCAG538550.032.6152086
TCAACGC545600.031.9181654
GTACTAG61000.030.9210821
ACGCAGA600900.028.7864177
CGCAGAG613600.028.1194678
GTACATG592250.025.803871
GCAGAGT662950.025.4602369
GCATCGT63350.025.1444918
TACATGG607000.024.9044382
ACATGGG606800.024.1610553
GTGGTAT92750.023.3283541
GAGTACT341500.022.73135612-13
TAGGCAT88300.022.6698655
CATGGGG404100.021.9775774
CTAGGCA93550.021.0965944
Produced by FastQC (version 0.11.5)