FastQCFastQC Report
Mon 6 Jun 2016
ERR1211287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12693919
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395470.3115428734026111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309970.2441877878691364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188120.14819694374920778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210150.052.8543241
GTATCAA399750.042.7761341
CAACGCA494350.033.8279575
ATCAACG495200.033.7765463
TATCAAC512050.033.1849862
AACGCAG534100.031.7581316
TCAACGC534400.031.4044044
ACGCAGA603750.027.6826577
GTACTAG58850.027.110911
CGCAGAG618800.027.0017978
GTACATG584700.025.393061
TACATGG602450.024.4364132
GCAGAGT676900.024.3992759
GTGGTAT96750.024.3965641
ACATGGG595650.023.8743293
TAGGCAT81950.022.0947535
GAGTACT353850.021.7908212-13
CATGGGG405850.021.5023274
TGGTATC106950.020.748462
GTACTTT377000.020.39057214-15
Produced by FastQC (version 0.11.5)