##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211287_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12693919 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14268942475527 33.0 31.0 34.0 30.0 34.0 2 32.277241094732055 34.0 31.0 34.0 30.0 34.0 3 32.3412548953558 34.0 31.0 34.0 30.0 34.0 4 35.97161428239774 37.0 35.0 37.0 35.0 37.0 5 35.87845526665169 37.0 35.0 37.0 35.0 37.0 6 35.89510741324251 37.0 35.0 37.0 35.0 37.0 7 35.813904122123354 37.0 35.0 37.0 35.0 37.0 8 35.79916320562625 37.0 35.0 37.0 33.0 37.0 9 37.416511244478556 39.0 37.0 39.0 34.0 39.0 10-11 37.370224041921176 39.0 37.0 39.0 34.0 39.0 12-13 37.297533212556345 39.0 37.0 39.0 34.0 39.0 14-15 38.53172999607135 40.0 38.0 41.0 34.0 41.0 16-17 38.520425528160374 40.0 38.0 41.0 34.0 41.0 18-19 38.535491167069836 40.0 38.0 41.0 34.0 41.0 20-21 38.50801529456742 40.0 38.0 41.0 34.0 41.0 22-23 38.40272424930394 40.0 38.0 41.0 34.0 41.0 24-25 38.30905640724507 40.0 38.0 41.0 34.0 41.0 26-27 38.20677270746725 40.0 38.0 41.0 33.5 41.0 28-29 38.11341729847181 40.0 37.5 41.0 33.0 41.0 30-31 38.11891343406241 40.0 37.5 41.0 33.0 41.0 32-33 38.056484762507154 40.0 37.0 41.0 33.0 41.0 34-35 37.9317199046252 40.0 37.0 41.0 33.0 41.0 36-37 37.791376721404944 40.0 37.0 41.0 33.0 41.0 38-39 37.67657305045038 40.0 37.0 41.0 33.0 41.0 40-41 37.568236413041554 40.0 36.5 41.0 32.5 41.0 42-43 37.46367489031559 40.0 36.0 41.0 32.0 41.0 44-45 37.29767591868201 40.0 36.0 41.0 31.5 41.0 46-47 37.051488905829636 39.5 36.0 41.0 31.0 41.0 48-49 35.49746094960902 39.0 35.0 41.0 24.5 41.0 50-51 36.22375611503429 39.0 35.0 40.0 29.0 41.0 52-53 36.31832497907069 39.0 35.0 40.0 30.0 41.0 54-55 36.15167053610473 38.5 35.0 40.0 29.0 41.0 56-57 35.93454227965375 38.0 35.0 40.0 29.0 41.0 58-59 35.692780102031534 38.0 34.5 40.0 28.0 41.0 60-61 35.439186511273626 37.5 34.0 40.0 28.0 41.0 62-63 35.12198636213135 37.0 34.0 40.0 28.0 41.0 64-65 34.796160665591145 36.5 34.0 39.5 27.0 41.0 66-67 34.464826268388826 36.0 33.5 39.0 26.5 40.5 68-69 34.11544003865158 35.5 33.0 39.0 26.0 40.0 70-71 33.774682822538885 35.0 33.0 38.0 26.0 40.0 72-73 33.41133853146535 35.0 32.5 37.5 26.0 40.0 74-75 33.05549030996653 35.0 32.0 37.0 25.5 39.0 76-77 32.11888755552954 34.0 30.5 36.0 24.5 38.5 78-79 32.55627860080091 35.0 32.0 36.0 25.0 38.5 80-81 32.43302734167439 35.0 32.0 36.0 25.0 37.5 82-83 32.16904235799834 34.5 32.0 35.0 25.0 37.0 84-85 31.890737801304702 34.0 31.0 35.0 24.5 36.5 86-87 31.638756202871626 34.0 31.0 35.0 24.0 36.0 88-89 31.418278429222685 34.0 31.0 35.0 24.0 36.0 90-91 31.206281606176944 34.0 31.0 35.0 23.0 35.0 92-93 31.021520776995665 34.0 30.5 35.0 23.0 35.0 94-95 30.83664103260782 34.0 30.0 35.0 21.0 35.0 96-97 30.655768600697705 34.0 30.0 35.0 20.0 35.0 98-99 30.450822161382945 34.0 29.5 35.0 20.0 35.0 100 27.986031027927623 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.27120149150941586 1101 2 -0.2242520922550142 1101 3 -0.26120277739485687 1101 4 -0.1873322492193168 1101 5 -0.15577460871666204 1101 6 -0.18328233332709232 1101 7 -0.2210867054225929 1101 8 -0.1873465992406267 1101 9 -0.3193029619886758 1101 10-11 -0.2601893062839409 1101 12-13 -0.34964059569552575 1101 14-15 -0.3916912691583363 1101 16-17 -0.4316795965539413 1101 18-19 -0.43679047388814496 1101 20-21 -0.46708223732745324 1101 22-23 -0.5557359201774474 1101 24-25 -0.4825809745637528 1101 26-27 -0.5983717137991889 1101 28-29 -0.6133664511999584 1101 30-31 -0.5000639628811285 1101 32-33 -0.4760182122340382 1101 34-35 -0.5444164661675899 1101 36-37 -0.551439562736256 1101 38-39 -0.5538724140873157 1101 40-41 -0.49865578477282213 1101 42-43 -0.501195008005709 1101 44-45 -0.448931354549849 1101 46-47 -0.4517996307743104 1101 48-49 0.8178893172550517 1101 50-51 -0.10334213427493921 1101 52-53 -0.44880022280403864 1101 54-55 -0.5834492145935926 1101 56-57 -0.4996702233464987 1101 58-59 -0.5797569333518311 1101 60-61 -0.6129225078438694 1101 62-63 -0.6196841011887599 1101 64-65 -0.6567469580507108 1101 66-67 -0.6410452858514901 1101 68-69 -0.6416932296217439 1101 70-71 -0.6450518491109491 1101 72-73 -0.7295557759094677 1101 74-75 -0.7548694313976014 1101 76-77 -0.6899343127521718 1101 78-79 -0.6232061278556458 1101 80-81 -0.492423229744432 1101 82-83 -0.4780801337261913 1101 84-85 -0.4924568938827001 1101 86-87 -0.49953612370368816 1101 88-89 -0.5296994907003416 1101 90-91 -0.5858566354707442 1101 92-93 -0.6085462292321502 1101 94-95 -0.5977955693654451 1101 96-97 -0.7266284134707881 1101 98-99 -0.963945951853816 1101 100 -0.8393824112948494 1102 1 -0.22693500172115932 1102 2 -0.23587355630262863 1102 3 -0.24236891105378078 1102 4 -0.1640772415221292 1102 5 -0.20106660461964765 1102 6 -0.18583849958693577 1102 7 -0.17458869219682782 1102 8 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1105 68-69 -0.752273435577159 1105 70-71 -0.7998372426835019 1105 72-73 -0.7217392171820691 1105 74-75 -0.8341874417593758 1105 76-77 -0.8215868284180203 1105 78-79 -0.6489285196065211 1105 80-81 -0.6075847764948463 1105 82-83 -0.5647669356194385 1105 84-85 -0.6341722478403895 1105 86-87 -0.6501505834748151 1105 88-89 -0.6694332790966264 1105 90-91 -0.7066594409103182 1105 92-93 -0.7678730666791509 1105 94-95 -0.6743640999487788 1105 96-97 -0.6313288842426523 1105 98-99 -0.7689899636713271 1105 100 -0.8196994631727534 1106 1 -0.29369885231018955 1106 2 -0.26651191787337325 1106 3 -0.3378937752603015 1106 4 -0.19482423071654154 1106 5 -0.24821755869844964 1106 6 -0.3359343007950386 1106 7 -0.326015002738373 1106 8 -0.34640840001175377 1106 9 -0.4425178100892708 1106 10-11 -0.4204765418771075 1106 12-13 -0.40614272142382646 1106 14-15 -0.5902894433409571 1106 16-17 -0.5916731559772046 1106 18-19 -0.5793817172368136 1106 20-21 -0.5757430031370561 1106 22-23 -0.6145280086986133 1106 24-25 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0.36884938065696815 2311 3 0.38539426996989334 2311 4 0.23784397590669926 2311 5 0.282355105392071 2311 6 0.3411970306208545 2311 7 0.350755301758646 2311 8 0.15133547403991088 2311 9 0.42719323027011313 2311 10-11 0.3351352824260232 2311 12-13 0.4422107733260745 2311 14-15 0.4395224297103866 2311 16-17 0.4301173477417919 2311 18-19 0.571906218149941 2311 20-21 0.6372706626437221 2311 22-23 0.6242510326961295 2311 24-25 0.6105655797375036 2311 26-27 0.6460177728400538 2311 28-29 0.6771374531238266 2311 30-31 0.5622725914243532 2311 32-33 0.5979750167281139 2311 34-35 0.7106872638363342 2311 36-37 0.7676573242971259 2311 38-39 0.7521949727070947 2311 40-41 0.6709978050602246 2311 42-43 0.6726323408401242 2311 44-45 0.7265785320570401 2311 46-47 0.8289553383332731 2311 48-49 -6.049325149276498 2311 50-51 -1.1511308016942365 2311 52-53 0.32806586513943614 2311 54-55 0.7637619279217773 2311 56-57 0.8758657101530929 2311 58-59 0.9041427336700139 2311 60-61 0.7574271560130015 2311 62-63 0.6069262184416147 2311 64-65 0.7255141971105985 2311 66-67 0.8101527477550761 2311 68-69 0.8333974286237193 2311 70-71 0.8202266873861532 2311 72-73 0.29796103663151285 2311 74-75 0.6411239064333998 2311 76-77 0.7852069006687827 2311 78-79 0.6928492766422494 2311 80-81 0.7229414311264506 2311 82-83 0.7119021074668979 2311 84-85 0.8064618114138362 2311 86-87 0.7979943401626279 2311 88-89 0.8489333057717765 2311 90-91 0.88756742444113 2311 92-93 0.8833008874905275 2311 94-95 0.9092989424731073 2311 96-97 0.9355929973617911 2311 98-99 1.0203021841330724 2311 100 1.2116538207611072 2312 1 0.07548660924771866 2312 2 0.21729898719605956 2312 3 0.19930411281762872 2312 4 0.012734432433639142 2312 5 0.20514782780225715 2312 6 0.12343338289186079 2312 7 0.2617530678713891 2312 8 0.29300031184278197 2312 9 0.22624756490212405 2312 10-11 0.19463914023496187 2312 12-13 0.27046066277399206 2312 14-15 0.2743717984728846 2312 16-17 0.4085509119251256 2312 18-19 0.268651701858893 2312 20-21 0.3181915909579729 2312 22-23 0.573363755779539 2312 24-25 0.6775402732448441 2312 26-27 0.5462527666480455 2312 28-29 0.6814096576872899 2312 30-31 0.709284000131909 2312 32-33 0.703396558896543 2312 34-35 0.284581556177244 2312 36-37 0.6087718502999806 2312 38-39 0.742388229838177 2312 40-41 0.5093436986169451 2312 42-43 0.5246268975703288 2312 44-45 0.644575400932851 2312 46-47 0.7425688885370434 2312 48-49 -4.738290336519945 2312 50-51 -0.8534117578874216 2312 52-53 0.43674785069737254 2312 54-55 0.7315381680767459 2312 56-57 0.838162369055901 2312 58-59 0.8810764320712963 2312 60-61 0.8882161175258076 2312 62-63 0.6968618184359343 2312 64-65 0.8363198368283165 2312 66-67 0.839633887855392 2312 68-69 0.7719819721970396 2312 70-71 0.761533860797563 2312 72-73 0.8677814209408155 2312 74-75 0.5635849194319391 2312 76-77 0.7650306367811126 2312 78-79 0.4468034502977929 2312 80-81 0.628020255763694 2312 82-83 0.679971064563702 2312 84-85 0.6783215505024138 2312 86-87 0.8035537443321736 2312 88-89 0.8358021244703728 2312 90-91 0.6429998258681096 2312 92-93 0.8372389628914831 2312 94-95 0.9677626446305467 2312 96-97 0.9554589316201536 2312 98-99 1.0284155039364613 2312 100 0.5439684435418464 2313 1 0.06188553811818309 2313 2 0.20095607669327364 2313 3 0.2965201378480202 2313 4 0.08407377112452963 2313 5 0.14891229300344833 2313 6 0.2461505205504153 2313 7 0.1630784600939137 2313 8 0.25396811521509477 2313 9 0.4380902073995827 2313 10-11 0.2326599471937314 2313 12-13 0.08194045504517788 2313 14-15 0.1781826295505482 2313 16-17 0.17113776682931103 2313 18-19 0.5018442010220738 2313 20-21 0.4307707066709767 2313 22-23 0.5311429248069786 2313 24-25 0.304047166220748 2313 26-27 0.47818072815464063 2313 28-29 0.45015350893174855 2313 30-31 0.6060269073861448 2313 32-33 0.5219447328136937 2313 34-35 0.42801769097037834 2313 36-37 -0.308872366979557 2313 38-39 0.19348930096771255 2313 40-41 0.4221374455097262 2313 42-43 0.49749480215724873 2313 44-45 0.5361465260105973 2313 46-47 0.3926674557356762 2313 48-49 -5.361872579598707 2313 50-51 -1.3110935102441346 2313 52-53 0.08795383086274455 2313 54-55 0.32875554666937745 2313 56-57 0.32544350202253014 2313 58-59 0.4627515919429186 2313 60-61 0.7716211418851486 2313 62-63 0.8412529511063696 2313 64-65 0.8892819206835654 2313 66-67 0.9339547626833777 2313 68-69 0.9622968442153379 2313 70-71 0.9173473270220427 2313 72-73 0.7466905285993306 2313 74-75 0.8128504725786811 2313 76-77 0.8258823363616372 2313 78-79 0.762010992792483 2313 80-81 -0.1584394162028886 2313 82-83 0.28233186024172596 2313 84-85 0.6537795760595806 2313 86-87 0.7840269966683415 2313 88-89 0.8026466571776858 2313 90-91 0.7908018358602646 2313 92-93 0.8253974430544702 2313 94-95 0.756138940825636 2313 96-97 0.8091867654460252 2313 98-99 0.9594564044470886 2313 100 1.0213702441936583 2314 1 0.049041379804414476 2314 2 0.1652452153542825 2314 3 0.22228817794421474 2314 4 0.05624910758348989 2314 5 0.19163056287570868 2314 6 0.27088677010276285 2314 7 0.05888622046001046 2314 8 0.19716057079956073 2314 9 0.413710623011184 2314 10-11 0.3036110307998072 2314 12-13 0.21250841046904156 2314 14-15 0.25163271908482443 2314 16-17 0.4448347686277927 2314 18-19 0.5165937853879257 2314 20-21 0.5624198357601671 2314 22-23 0.2625453407901901 2314 24-25 0.39292010000427524 2314 26-27 0.46049639923840147 2314 28-29 0.5864077207223275 2314 30-31 0.49932931455099094 2314 32-33 0.48096909266374155 2314 34-35 0.3329397390070028 2314 36-37 0.411542902666163 2314 38-39 0.4321322194150099 2314 40-41 0.24755416523749574 2314 42-43 0.284332431023131 2314 44-45 0.49111579030806496 2314 46-47 0.6274008933839781 2314 48-49 -6.348290079167057 2314 50-51 -1.5447987875684817 2314 52-53 -0.8942167842566917 2314 54-55 -0.47250144836937835 2314 56-57 -0.08693600677170821 2314 58-59 0.38113064445944644 2314 60-61 0.594790427869377 2314 62-63 0.5187621188802041 2314 64-65 0.6868329918262503 2314 66-67 0.7257247313380688 2314 68-69 0.7577388894192367 2314 70-71 0.5050212948389188 2314 72-73 0.6819316492276286 2314 74-75 0.683270081676838 2314 76-77 0.6364155031891414 2314 78-79 0.452510098404872 2314 80-81 0.534943213997785 2314 82-83 0.5104717969894637 2314 84-85 0.5476262105453351 2314 86-87 0.5971161110062546 2314 88-89 0.5533135411321908 2314 90-91 0.6978671655285495 2314 92-93 0.7649478820870854 2314 94-95 0.8394685130773176 2314 96-97 0.8413521327222604 2314 98-99 0.8927037482303319 2314 100 -0.5392454252433403 2315 1 0.161171108673976 2315 2 0.22528756437134234 2315 3 0.3243051191111448 2315 4 0.147789626858831 2315 5 0.2358882368961801 2315 6 0.28445102739715367 2315 7 0.33396511620815517 2315 8 0.24935771045329602 2315 9 0.4136137665682966 2315 10-11 0.23992020033576722 2315 12-13 0.41676650896775413 2315 14-15 0.27035490698476394 2315 16-17 0.38144715083345204 2315 18-19 0.47049200369740873 2315 20-21 0.37043544745866086 2315 22-23 0.44989924357179945 2315 24-25 0.47331882937775305 2315 26-27 0.5290167584626957 2315 28-29 0.6266933380215889 2315 30-31 0.6059959825177472 2315 32-33 0.7313728345206556 2315 34-35 0.7226284573505524 2315 36-37 0.6210982685391002 2315 38-39 0.7110930433890132 2315 40-41 0.7193264437794653 2315 42-43 0.7615271427653596 2315 44-45 0.7868270260194734 2315 46-47 0.7371625959824399 2315 48-49 -4.881634583493483 2315 50-51 -0.840564756920763 2315 52-53 0.3238273012858812 2315 54-55 0.2693830867899081 2315 56-57 0.6244225302981761 2315 58-59 0.7712028233107873 2315 60-61 0.6508032238405619 2315 62-63 0.46414100048340856 2315 64-65 0.6017370662452848 2315 66-67 -0.1339810187884325 2315 68-69 0.6191539119965412 2315 70-71 0.6140442070430367 2315 72-73 0.7054621744781642 2315 74-75 0.8438272519397998 2315 76-77 0.8298020347612223 2315 78-79 0.7358195979027826 2315 80-81 0.5988384018056792 2315 82-83 0.548705049015382 2315 84-85 0.6206719432974701 2315 86-87 0.6671679396038819 2315 88-89 0.6506044079206497 2315 90-91 0.7048479798923211 2315 92-93 0.7876167912729244 2315 94-95 0.38024796822667284 2315 96-97 0.5406812133427046 2315 98-99 0.5235665044708782 2315 100 0.5907772313637771 2316 1 0.2214305347062222 2316 2 0.2352703488113974 2316 3 0.20005928850801524 2316 4 -0.03831878695087454 2316 5 0.1518166874428104 2316 6 0.09650203339533903 2316 7 0.21435077263534197 2316 8 0.27733473354093263 2316 9 0.41978474174784 2316 10-11 0.3916893374991659 2316 12-13 0.2692185187274774 2316 14-15 0.43031549233587896 2316 16-17 0.4452859063557284 2316 18-19 0.09266761153197223 2316 20-21 0.4343879252588394 2316 22-23 0.5588618778828263 2316 24-25 0.566433868113684 2316 26-27 0.5395363243780764 2316 28-29 0.6097938792018667 2316 30-31 0.5477716936942301 2316 32-33 0.4701782877308034 2316 34-35 0.47840554079087383 2316 36-37 0.3659420109747842 2316 38-39 0.48634613948964756 2316 40-41 0.11524090001600484 2316 42-43 0.08681410953657576 2316 44-45 0.29133013576805666 2316 46-47 0.3640511509643005 2316 48-49 -5.123788169368289 2316 50-51 -1.0995061026200048 2316 52-53 0.18779042799030776 2316 54-55 0.4681275810700498 2316 56-57 0.33929333193157873 2316 58-59 0.20866135981235345 2316 60-61 0.4073786348207662 2316 62-63 0.3604005137743158 2316 64-65 0.3664831361843497 2316 66-67 0.44099060559261005 2316 68-69 0.13271804001249876 2316 70-71 -0.1667411047197973 2316 72-73 -0.09882669171398817 2316 74-75 0.07256247811410077 2316 76-77 0.18526655138722958 2316 78-79 0.2858511969788111 2316 80-81 0.16767315251592407 2316 82-83 0.13730514179944464 2316 84-85 0.2617870168338676 2316 86-87 0.3173248608049022 2316 88-89 0.13739147118406336 2316 90-91 0.11724455152454638 2316 92-93 0.22887652399802505 2316 94-95 0.40095325842646545 2316 96-97 0.4407020687880667 2316 98-99 0.4432210736610358 2316 100 -0.27045593989113215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 12.0 11 19.0 12 51.0 13 253.0 14 958.0 15 2767.0 16 6539.0 17 13549.0 18 23851.0 19 36043.0 20 53414.0 21 76673.0 22 96302.0 23 100323.0 24 101611.0 25 110759.0 26 131379.0 27 159512.0 28 197829.0 29 243351.0 30 300735.0 31 373839.0 32 472731.0 33 619424.0 34 869809.0 35 1229344.0 36 1923949.0 37 2859508.0 38 2362161.0 39 327201.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67332011574322 20.61180473689851 14.444538130330903 23.270337017027366 2 15.173101184101622 20.575374789048468 40.64931082994569 23.60221319690422 3 18.38335365279156 26.33294648109944 29.97407890462102 25.309620961487983 4 12.604130454172829 17.157307545992953 38.84157735871178 31.396984641122433 5 13.268340211201387 38.27444552703292 34.58391209101474 13.873302170750954 6 30.51156160163656 37.78875644048692 17.814920155450846 13.884761802425666 7 27.497558672989484 32.63342060873886 22.25838740385552 17.610633314416134 8 26.29201297773832 35.44877432252546 19.81098251701727 18.44823018271895 9 26.544002793432973 16.037390528957523 20.697167000745157 36.72143967686435 10-11 25.08388272052786 26.525598454264692 27.67117666315615 20.719342162051298 12-13 25.611063859655815 24.020997791341852 28.236989141207243 22.130949207795094 14-15 23.057428556742103 25.44015916303123 25.804495504829976 25.69791677539669 16-17 22.070783091598713 28.452918134795517 26.707324845305365 22.768973928300404 18-19 21.799131962114764 27.635274252641974 29.057215283290045 21.508378501953217 20-21 22.826142614844443 26.64591524597228 28.85619954765711 21.671742591526172 22-23 22.87474908683383 26.452476107882983 28.59632751474362 22.076447290539576 24-25 22.470482904604292 26.73874056979269 28.84291804380288 21.94785848180014 26-27 22.340960258070197 26.910714393330565 28.544580489991638 22.203744858607596 28-29 22.015584468376353 26.990256339476808 28.82421974458066 22.169939447566183 30-31 22.63424013179559 26.660967467374885 28.66110990140225 22.04368249942727 32-33 22.640124669938622 26.97188899301686 28.30703675297208 22.08094958407244 34-35 22.607112742888564 26.98332917767974 28.65097871650068 21.758579362931016 36-37 22.591650133208606 26.753078091331822 28.507591233899404 22.147680541560167 38-39 22.59973096411983 26.76600103189254 28.72250348350406 21.91176452048357 40-41 22.478291822179948 26.361473485788643 29.025214097924216 22.13502059410719 42-43 22.37075916842577 26.49690479203889 28.60871610467311 22.523619934862232 44-45 22.2927248078284 26.961718129413565 28.47735411316257 22.268202949595466 46-47 22.40212533174824 26.732517526112144 28.127350197285097 22.738006944854522 48-49 22.84660266521619 26.78217241753637 28.012393020460475 22.358831896786967 50-51 22.63868286869202 26.821880834083565 27.812758094302193 22.72667820292222 52-53 22.332797384836077 27.125254047624566 27.77262341667876 22.7693251508606 54-55 22.332414260980702 27.08533916314584 27.70691743463826 22.875329141235195 56-57 22.65526323253205 26.8673855428521 27.523461824507443 22.95388940010841 58-59 22.26026885972447 26.94964583156972 27.964768339217 22.825316969488814 60-61 22.39974887487657 27.280518800744396 27.64046927530281 22.679263049076226 62-63 22.506268633857342 26.9640124046745 27.573693033073383 22.956025928394777 64-65 22.037913889073614 26.87366780328543 27.67856045379864 23.40985785384232 66-67 22.297699980906525 26.74357588876266 27.60641105785654 23.352313072474278 68-69 22.023329258522292 26.85602678421917 27.70832106989361 23.412322887364926 70-71 22.09046188267892 26.565551791118263 27.77559395674711 23.56839236945571 72-73 22.287700985456745 26.84646671554132 27.562972343795998 23.30285995520594 74-75 22.294956039709422 27.01275260080495 27.467620180253142 23.22467117923248 76-77 22.115454465375684 26.89588603371217 27.43005967365042 23.55859982726173 78-79 21.99458916677588 26.74026546952208 27.526612481125724 23.738532882576315 80-81 21.860039795731264 27.130892426068392 27.44462601052619 23.56444176767415 82-83 22.294376830713688 26.68623419914399 27.817334276806392 23.20205469333593 84-85 22.35264842223276 26.789718512883653 27.28493830919405 23.572694755689533 86-87 21.72764549234775 27.079039576994806 27.649262763546943 23.5440521671105 88-89 21.973629047764646 26.562457642879433 27.608355424705984 23.855557884649937 90-91 22.041113123692213 26.495097892442022 27.651510376104028 23.81227860776174 92-93 22.092930954329184 26.64699688892326 27.44947607510564 23.810596081641922 94-95 21.53393186773198 26.450362385021325 27.76774758144421 24.247958165802487 96-97 21.71871544111782 26.341827535237133 27.70516318931891 24.234293834326134 98-99 21.401047813111262 26.530370648878606 27.627447238637252 24.44113429937288 100 21.527506504324197 26.379458510823987 27.37933754595778 24.713697438894027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2163.0 1 1520.0 2 1179.5 3 1537.5 4 1239.5 5 1077.0 6 1795.0 7 3508.0 8 5942.0 9 7449.0 10 6845.5 11 6135.5 12 6734.5 13 7765.0 14 8990.5 15 10364.0 16 11649.5 17 12950.0 18 14382.0 19 15612.5 20 16849.0 21 18615.5 22 20822.5 23 23783.0 24 27747.0 25 32595.5 26 39812.0 27 48942.5 28 60042.0 29 78361.5 30 103684.5 31 131794.5 32 168316.5 33 210641.0 34 253981.0 35 297376.0 36 348329.5 37 408242.5 38 435762.0 39 430995.0 40 415052.5 41 400141.5 42 404144.5 43 421722.5 44 441795.5 45 475903.5 46 506063.5 47 517145.0 48 527932.0 49 527677.5 50 515254.5 51 503834.5 52 488122.0 53 464730.0 54 442432.5 55 404410.5 56 360029.5 57 309632.5 58 261479.5 59 230716.5 60 190284.0 61 147236.5 62 115922.0 63 91636.5 64 67267.0 65 46389.5 66 33714.5 67 24981.0 68 18778.5 69 14081.5 70 10762.5 71 8158.0 72 6020.0 73 4676.0 74 3675.0 75 2781.5 76 1908.5 77 1126.0 78 693.0 79 442.0 80 261.0 81 156.5 82 102.0 83 60.0 84 40.5 85 22.5 86 18.5 87 17.0 88 9.5 89 6.5 90 5.0 91 5.0 92 3.5 93 1.0 94 1.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0296126042713838 2 0.0019300580065147728 3 8.665566559862246E-5 4 0.02069494850250738 5 4.963006302466559E-4 6 2.3633363345078853E-5 7 2.3633363345078853E-5 8 0.0038601160130295456 9 0.010185979601728986 10-11 0.001217118212271561 12-13 9.847234727116189E-5 14-15 1.299834983979337E-4 16-17 3.15111511267718E-4 18-19 2.3633363345078853E-5 20-21 7.090009003523657E-5 22-23 2.796614662500998E-4 24-25 3.1511151126771806E-5 26-27 6.696119614439009E-5 28-29 0.0010004790482750047 30-31 0.002544525453486823 32-33 0.009484856489158313 34-35 0.018697929299848218 36-37 0.021541810689039376 38-39 0.021073082316028644 40-41 0.0047542449262516955 42-43 0.0052663011320617376 44-45 0.009150050508436362 46-47 0.011820620566430273 48-49 1.1963169136340006 50-51 0.018410390045816426 52-53 0.018914568463844775 54-55 0.009685740077591482 56-57 0.0075547984826435394 58-59 0.008015649067872577 60-61 0.005081173119191953 62-63 0.0075784318459886195 64-65 0.019497524759690053 66-67 0.02930537054789778 68-69 0.029620482059165495 70-71 0.026544205930414396 72-73 0.023247351743775898 74-75 0.009563634366975242 76-77 0.01484962996849121 78-79 0.008267738276886752 80-81 0.0027296534663566073 82-83 0.01422728473373747 84-85 0.02433842535154037 86-87 0.023720019010677475 88-89 0.0330788308953287 90-91 0.02082099310701447 92-93 0.01330952245717024 94-95 0.0067630808105833985 96-97 0.003596210122342832 98-99 0.015448341839899876 100 0.0337484428567726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2693919E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.62561091016477 #Duplication Level Percentage of deduplicated Percentage of total 1 69.07495253245959 33.5881176548148 2 15.509741147412845 15.083412767029387 3 6.292601189310755 9.179447311327946 4 3.1282014459232768 6.0844282543232024 5 1.752474863040988 4.260758041003768 6 1.0638436378849516 3.103802808302873 7 0.6971779772788491 2.3730493540807904 8 0.48384676924413905 1.8821875793124627 9 0.35567512829375614 1.556542835895161 >10 1.524615385618048 12.407460845585964 >50 0.06942772532246401 2.3147440840361075 >100 0.041220668888048255 3.929759668941999 >500 0.004129785300749795 1.359639570161119 >1k 0.001983081997477196 1.6034090206543756 >5k 2.7165505970547278E-5 0.09290166262186682 >10k+ 8.149651791164183E-5 1.1803385419080805 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39547 0.3115428734026111 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30997 0.2441877878691364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18812 0.14819694374920778 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5755575563385903E-5 2 0.0 0.0 0.0 0.0 1.5755575563385903E-5 3 0.0 0.0 0.0 0.0 1.5755575563385903E-5 4 0.0 0.0 0.0 0.0 1.5755575563385903E-5 5 0.0 0.0 0.0 0.0 1.5755575563385903E-5 6 0.0 0.0 0.0 0.0 2.3633363345078853E-5 7 0.0 0.0 0.0 0.0 2.3633363345078853E-5 8 0.0 0.0 0.0 0.0 2.3633363345078853E-5 9 0.0 0.0 0.0 0.0 2.3633363345078853E-5 10-11 0.0 0.0 0.0 3.938893890846476E-6 2.3633363345078853E-5 12-13 0.0 0.0 0.0 7.877787781692952E-6 2.3633363345078853E-5 14-15 0.0 3.938893890846476E-6 0.0 7.877787781692952E-6 3.938893890846475E-5 16-17 0.0 7.877787781692952E-6 0.0 3.15111511267718E-5 5.514451447185065E-5 18-19 0.0 7.877787781692952E-6 0.0 4.332783279931123E-5 6.302230225354361E-5 20-21 0.0 7.877787781692952E-6 0.0 1.299834983979337E-4 6.696119614439008E-5 22-23 0.0 1.1816681672539426E-5 0.0 5.238728874825812E-4 7.483898392608303E-5 24-25 0.0 1.5755575563385903E-5 0.0 0.0012880183023067974 9.059455948946894E-5 26-27 0.0 1.5755575563385903E-5 0.0 0.002599669967958674 9.453345338031541E-5 28-29 0.0 1.5755575563385903E-5 0.0 0.006365252527607904 9.453345338031541E-5 30-31 0.0 1.5755575563385903E-5 0.0 0.017638366843210518 1.0635013505285484E-4 32-33 0.0 1.5755575563385903E-5 0.0 0.043170277043677374 1.2604460450708722E-4 34-35 0.0 1.9694469454232376E-5 0.0 0.08229924895534627 1.2998349839793367E-4 36-37 0.0 2.3633363345078853E-5 0.0 0.1438090159548048 1.3392239228878015E-4 38-39 0.0 2.3633363345078853E-5 0.0 0.23036227031226525 1.3392239228878015E-4 40-41 0.0 2.3633363345078853E-5 0.0 0.3261916197826692 1.3392239228878015E-4 42-43 0.0 2.3633363345078853E-5 0.0 0.42094565122087196 1.3786128617962663E-4 44-45 0.0 2.3633363345078853E-5 0.0 0.5367215593545225 1.5361686174301253E-4 46-47 0.0 2.3633363345078853E-5 0.0 0.6753469909489733 1.6543354341555196E-4 48-49 0.0 2.3633363345078853E-5 0.0 0.8254779315985867 1.8118911897893787E-4 50-51 0.0 2.3633363345078853E-5 0.0 0.9894540842745254 1.8118911897893787E-4 52-53 0.0 2.3633363345078853E-5 0.0 1.1524061245388442 2.1270027010570968E-4 54-55 0.0 2.3633363345078853E-5 0.0 1.3298729887909322 2.442114212324815E-4 56-57 0.0 2.3633363345078853E-5 0.0 1.5351681383818505 2.442114212324815E-4 58-59 0.0 2.3633363345078853E-5 0.0 1.7685869903534126 2.442114212324815E-4 60-61 0.0 2.3633363345078853E-5 0.0 2.0093361238558396 2.599669967958674E-4 62-63 0.0 2.3633363345078853E-5 0.0 2.2540517235063495 2.678447845775603E-4 64-65 0.0 2.3633363345078853E-5 0.0 2.5065939053179713 2.7572257235925325E-4 66-67 0.0 2.3633363345078853E-5 0.0 2.7863853550664692 2.9541704181348564E-4 68-69 0.0 2.3633363345078853E-5 0.0 3.0951513082760336 3.0723372348602507E-4 70-71 0.0 2.3633363345078853E-5 0.0 3.430662351004446 3.190504051585645E-4 72-73 0.0 2.3633363345078853E-5 0.0 3.76497596999004 4.175227524297264E-4 74-75 0.0 2.3633363345078853E-5 0.0 4.094948927907922 4.175227524297264E-4 76-77 0.0 2.3633363345078853E-5 0.0 4.453853061454071 4.2933943410226584E-4 78-79 0.0 2.3633363345078853E-5 0.0 4.843208783670354 4.332783279931123E-4 80-81 0.0 2.3633363345078853E-5 0.0 5.259400189964975 4.332783279931123E-4 82-83 3.938893890846476E-6 2.3633363345078853E-5 0.0 5.682681605263118 4.608505852290376E-4 84-85 7.877787781692952E-6 2.3633363345078853E-5 0.0 6.0960645802135645 4.647894791198841E-4 86-87 7.877787781692952E-6 2.3633363345078853E-5 0.0 6.523261256039211 4.647894791198841E-4 88 7.877787781692952E-6 2.3633363345078853E-5 0.0 6.8582917537129395 4.647894791198841E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21015 0.0 52.854324 1 GTATCAA 39975 0.0 42.776134 1 CAACGCA 49435 0.0 33.827957 5 ATCAACG 49520 0.0 33.776546 3 TATCAAC 51205 0.0 33.184986 2 AACGCAG 53410 0.0 31.758131 6 TCAACGC 53440 0.0 31.404404 4 ACGCAGA 60375 0.0 27.682657 7 GTACTAG 5885 0.0 27.11091 1 CGCAGAG 61880 0.0 27.001797 8 GTACATG 58470 0.0 25.39306 1 TACATGG 60245 0.0 24.436413 2 GCAGAGT 67690 0.0 24.399275 9 GTGGTAT 9675 0.0 24.396564 1 ACATGGG 59565 0.0 23.874329 3 TAGGCAT 8195 0.0 22.094753 5 GAGTACT 35385 0.0 21.79082 12-13 CATGGGG 40585 0.0 21.502327 4 TGGTATC 10695 0.0 20.74846 2 GTACTTT 37700 0.0 20.390572 14-15 >>END_MODULE