FastQCFastQC Report
Mon 6 Jun 2016
ERR1211286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581040
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT541500.5651787279877758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389700.40674081310588417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236940.24730091931564843No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG133210.13903501081302239No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT107960.11268087806751668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206700.055.6601031
GTATCAA416600.042.98251
GCATCGT41400.035.6401828
ATCAACG502750.034.478913
CAACGCA504350.034.360755
TATCAAC544050.032.3154642
AACGCAG545850.032.257116
TACCTGG28550.031.9599082
TCAACGC549450.031.6768534
TAGGCAT64750.031.6410185
GTACTAG58150.029.6855031
ACCTGGG30600.029.6369043
ACGCAGA601150.028.8529287
CGCAGAG605400.028.5870868
CATGGGT53750.028.3247284
GTACATG582950.028.0624961
TACCCCG5850.027.310325
CTGTGCG52150.027.3038469
ATGGGTA39000.027.2299985
ACATGGG589750.026.5560993
Produced by FastQC (version 0.11.5)