##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211286_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9581040 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.571253120746807 31.0 31.0 34.0 30.0 34.0 2 31.597475430642184 33.0 31.0 34.0 30.0 34.0 3 31.638347924651185 33.0 31.0 34.0 28.0 34.0 4 35.26609251187762 37.0 35.0 37.0 33.0 37.0 5 35.3321020473769 37.0 35.0 37.0 33.0 37.0 6 35.39850903450982 37.0 35.0 37.0 33.0 37.0 7 35.345923198316676 37.0 35.0 37.0 32.0 37.0 8 35.352306221454036 37.0 35.0 37.0 32.0 37.0 9 36.92439682957174 39.0 37.0 39.0 33.0 39.0 10-11 36.865702940390605 39.0 37.0 39.0 33.0 39.0 12-13 36.80673465511051 39.0 37.0 39.0 32.0 39.0 14-15 37.93142179763366 40.0 38.0 41.0 32.5 41.0 16-17 37.95165002964188 40.0 38.0 41.0 32.5 41.0 18-19 38.020399977455476 40.0 38.0 41.0 33.0 41.0 20-21 38.01061372251864 40.0 38.0 41.0 33.0 41.0 22-23 37.889445248115024 40.0 38.0 41.0 32.0 41.0 24-25 37.78914778562661 40.0 37.0 41.0 32.0 41.0 26-27 37.64442664888154 40.0 37.0 41.0 32.0 41.0 28-29 37.54536162045039 40.0 37.0 41.0 32.0 41.0 30-31 37.366937253158326 40.0 36.5 41.0 31.5 41.0 32-33 37.23618276304033 40.0 36.0 41.0 31.0 41.0 34-35 37.115518461461384 39.5 36.0 41.0 31.0 41.0 36-37 37.02681478211133 39.0 36.0 41.0 30.5 41.0 38-39 36.88874255821915 39.0 35.0 41.0 30.0 41.0 40-41 36.749219239247516 39.0 35.0 41.0 30.0 41.0 42-43 36.60988024264589 39.0 35.0 41.0 30.0 41.0 44-45 36.44506801975568 39.0 35.0 41.0 29.5 41.0 46-47 36.243295560815945 39.0 35.0 41.0 28.5 41.0 48-49 35.9842832823994 39.0 35.0 41.0 27.5 41.0 50-51 35.30179155916268 38.5 34.0 39.5 26.5 40.5 52-53 35.52694623965665 38.5 34.5 40.0 27.0 40.5 54-55 35.90741887102027 39.0 35.0 40.5 28.0 41.0 56-57 35.82806052370098 39.0 35.0 41.0 27.0 41.0 58-59 35.6295460618054 38.5 35.0 40.5 27.0 41.0 60-61 35.40993028940491 38.0 34.0 40.0 26.0 41.0 62-63 35.16355807928993 38.0 34.0 40.0 26.0 41.0 64-65 34.93008723478871 37.5 34.0 40.0 26.0 41.0 66-67 34.74398076826733 37.0 34.0 40.0 26.0 41.0 68-69 34.43545152718285 36.5 34.0 39.5 26.0 41.0 70-71 34.13814549359986 36.0 33.0 39.0 25.0 41.0 72-73 33.79879433756669 36.0 33.0 39.0 25.0 41.0 74-75 33.4134757813348 35.0 33.0 38.5 24.0 40.0 76-77 33.06158861668462 35.0 32.5 37.0 24.0 39.5 78-79 32.68664565642143 35.0 32.0 37.0 23.0 39.0 80-81 32.33965806426025 35.0 32.0 36.5 22.5 39.0 82-83 31.998392397902524 35.0 31.5 36.0 21.5 37.5 84-85 31.652927030885998 35.0 31.0 35.5 20.0 37.0 86-87 31.40431597196129 35.0 31.0 35.0 20.0 37.0 88-89 31.129640519192073 34.0 31.0 35.0 20.0 36.0 90-91 30.85838405851557 34.0 30.5 35.0 19.0 36.0 92-93 30.616694012341043 34.0 30.0 35.0 18.5 36.0 94-95 30.40323534814592 34.0 30.0 35.0 18.0 35.5 96-97 30.211621546303952 34.0 29.5 35.0 17.5 35.0 98-99 29.988475833521203 34.0 29.0 35.0 16.5 35.0 100 28.335126353715253 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.36078074491993917 1101 2 -0.4015462943134267 1101 3 -0.34485901040249445 1101 4 -0.26622566550548754 1101 5 -0.28282282347958443 1101 6 -0.3527799891912409 1101 7 -0.3539627353584862 1101 8 -0.4356966686817856 1101 9 -0.4273387569910554 1101 10-11 -0.49193260358622837 1101 12-13 -0.4767418856535457 1101 14-15 -0.5252670470381915 1101 16-17 -0.5602157605711113 1101 18-19 -0.6244714700667444 1101 20-21 -0.6351842630894851 1101 22-23 -0.5849548421498909 1101 24-25 -0.5806125025109807 1101 26-27 -0.6231621616605167 1101 28-29 -0.6847328029497106 1101 30-31 -0.6561702582613336 1101 32-33 -0.6231198700849419 1101 34-35 -0.7034788029368855 1101 36-37 -0.6842542378768997 1101 38-39 -0.6927694856630922 1101 40-41 -0.7519486519323735 1101 42-43 -0.7984434981635147 1101 44-45 -0.7998846250709946 1101 46-47 -0.6997534174276936 1101 48-49 -0.704084376664639 1101 50-51 -0.6843286695105135 1101 52-53 -0.42383455302593376 1101 54-55 -0.40642096407598416 1101 56-57 -0.39641264092463757 1101 58-59 -0.46599891475469235 1101 60-61 -0.4339302138602079 1101 62-63 -0.412681310010079 1101 64-65 -0.43710160387015407 1101 66-67 -0.49642445413640957 1101 68-69 -0.49831316879240006 1101 70-71 -0.4084553252613219 1101 72-73 -0.50446489994534 1101 74-75 -0.46488395055371967 1101 76-77 -0.46241549988585007 1101 78-79 -0.47697951751754175 1101 80-81 -0.5297108779958606 1101 82-83 -0.5872220558786694 1101 84-85 -0.517474750108974 1101 86-87 -0.5323649673746829 1101 88-89 -0.5836872689831409 1101 90-91 -0.640848222242699 1101 92-93 -0.5799362167795898 1101 94-95 -0.6837632200935779 1101 96-97 -0.8480126425875092 1101 98-99 -0.751633559110477 1101 100 -0.6990980241101425 1102 1 -0.4538400726284699 1102 2 -0.45404251587510913 1102 3 -0.48913625381572245 1102 4 -0.2766450510568319 1102 5 -0.25932948307060144 1102 6 -0.3424584131482078 1102 7 -0.4125079826293643 1102 8 -0.35331571070184253 1102 9 -0.44782631717648513 1102 10-11 -0.42814015385239657 1102 12-13 -0.49292725648455615 1102 14-15 -0.5911349686321898 1102 16-17 -0.6489617185802175 1102 18-19 -0.595234786427774 1102 20-21 -0.597029743850392 1102 22-23 -0.6408132896231891 1102 24-25 -0.691815234862915 1102 26-27 -0.6971444074503523 1102 28-29 -0.6855964216657711 1102 30-31 -0.6438548342808801 1102 32-33 -0.7311745208552409 1102 34-35 -0.7444304519874265 1102 36-37 -0.7333911433783484 1102 38-39 -0.8742812243885822 1102 40-41 -0.8876982167652088 1102 42-43 -0.9064279274821203 1102 44-45 -0.8598901290585914 1102 46-47 -0.9187598721444346 1102 48-49 -0.8592464041495731 1102 50-51 -0.8438783012240236 1102 52-53 -0.7869293551547045 1102 54-55 -0.6443388909254892 1102 56-57 -0.6032000521582574 1102 58-59 -0.6928987448756416 1102 60-61 -0.6830052291180522 1102 62-63 -0.6766401040266956 1102 64-65 -0.7297451871177216 1102 66-67 -0.6767322770223316 1102 68-69 -0.7439605716308364 1102 70-71 -0.8094934343631834 1102 72-73 -0.7580845921727999 1102 74-75 -0.7748154791592228 1102 76-77 -0.8012721359063306 1102 78-79 -0.7455926891604747 1102 80-81 -0.8182885547829599 1102 82-83 -0.8342813956116188 1102 84-85 -0.8175719880128867 1102 86-87 -0.8677846394686384 1102 88-89 -0.9186880483494413 1102 90-91 -0.9525845843171794 1102 92-93 -0.8169961150855443 1102 94-95 -0.8742377527336807 1102 96-97 -0.8975260295475991 1102 98-99 -0.861843721164469 1102 100 -0.9010891413778666 1103 1 -0.50859504971978 1103 2 -0.5372281072894793 1103 3 -0.5142875651479493 1103 4 -0.4167938968863538 1103 5 -0.39476086882487493 1103 6 -0.5084363890664321 1103 7 -0.4920956314087803 1103 8 -0.5490756165511641 1103 9 -0.6941586622150098 1103 10-11 -0.62733438942238 1103 12-13 -0.6756459078496846 1103 14-15 -0.7676380580162743 1103 16-17 -0.8315405779303973 1103 18-19 -0.7408238444939528 1103 20-21 -0.8161614598961648 1103 22-23 -0.8409112787330244 1103 24-25 -0.7924693969524483 1103 26-27 -0.8441499050286723 1103 28-29 -0.7705226242747116 1103 30-31 -0.8378638860209477 1103 32-33 -0.8867744865450717 1103 34-35 -0.9190849545854363 1103 36-37 -0.9575721049376469 1103 38-39 -0.9892167251437982 1103 40-41 -0.9911303264673634 1103 42-43 -1.0019212524728331 1103 44-45 -1.0542835998357987 1103 46-47 -1.0873105340038407 1103 48-49 -0.9661028311593043 1103 50-51 -1.0008894264988157 1103 52-53 -0.8037020199012588 1103 54-55 -0.8795364745887753 1103 56-57 -0.837808639414547 1103 58-59 -0.8266907384487965 1103 60-61 -0.776264712717257 1103 62-63 -0.8982237591930797 1103 64-65 -0.924632132999804 1103 66-67 -0.8714394820815627 1103 68-69 -0.7837055224789609 1103 70-71 -0.8077903156938717 1103 72-73 -0.9471364045521042 1103 74-75 -0.8805471520205188 1103 76-77 -0.88448903231874 1103 78-79 -0.8374330896823565 1103 80-81 -0.8361741316584386 1103 82-83 -0.8809869184125816 1103 84-85 -0.8423429789144734 1103 86-87 -0.959235232978557 1103 88-89 -1.0063398891150186 1103 90-91 -0.9010198861783252 1103 92-93 -0.9023331867898037 1103 94-95 -1.0518492378560431 1103 96-97 -1.1127568518291966 1103 98-99 -1.157855887960725 1103 100 -1.0968457193603882 1104 1 -0.3365162405609148 1104 2 -0.37670818550706997 1104 3 -0.42091006742720793 1104 4 -0.22299765402042482 1104 5 -0.1945714125613378 1104 6 -0.19741596809173245 1104 7 -0.21268455990688295 1104 8 -0.2721988199783354 1104 9 -0.39568261171628194 1104 10-11 -0.3266594745218683 1104 12-13 -0.3996042725989568 1104 14-15 -0.5148634302099779 1104 16-17 -0.49793969249797954 1104 18-19 -0.5136132179646253 1104 20-21 -0.46409632407095813 1104 22-23 -0.5021518123426674 1104 24-25 -0.563743097974573 1104 26-27 -0.5505765561554767 1104 28-29 -0.6265904299799985 1104 30-31 -0.5670161013556765 1104 32-33 -0.6406798647527623 1104 34-35 -0.5327972495943385 1104 36-37 -0.5698484820951322 1104 38-39 -0.6012948328196543 1104 40-41 -0.5790684824740637 1104 42-43 -0.5718555900885676 1104 44-45 -0.5745013064286866 1104 46-47 -0.6484076098696718 1104 48-49 -0.6544167273813741 1104 50-51 -0.5611256218604979 1104 52-53 -0.48462306150970136 1104 54-55 -0.5265801593315942 1104 56-57 -0.4222671660187416 1104 58-59 -0.41096470688340503 1104 60-61 -0.42723377016759656 1104 62-63 -0.5314748972599048 1104 64-65 -0.5082720858976799 1104 66-67 -0.4237736891410293 1104 68-69 -0.4045502409485877 1104 70-71 -0.4788669145110447 1104 72-73 -0.45154630877733126 1104 74-75 -0.46606940835626176 1104 76-77 -0.4219538101642044 1104 78-79 -0.43566268842290157 1104 80-81 -0.4738318787675553 1104 82-83 -0.5442353354402094 1104 84-85 -0.5327134881665643 1104 86-87 -0.5879558296144296 1104 88-89 -0.541555788383846 1104 90-91 -0.5552461761084011 1104 92-93 -0.5277768915494576 1104 94-95 -0.5409929784270737 1104 96-97 -0.6125689426261225 1104 98-99 -0.68379554009217 1104 100 -0.7920189018882766 1105 1 -0.3482658333009141 1105 2 -0.3632426202377488 1105 3 -0.36905292403011103 1105 4 -0.2510527654641521 1105 5 -0.23472053964535178 1105 6 -0.3068626047477778 1105 7 -0.27550795836402386 1105 8 -0.30979477648183007 1105 9 -0.4681574107320543 1105 10-11 -0.462704394071487 1105 12-13 -0.46849050626602917 1105 14-15 -0.4969957245106045 1105 16-17 -0.5523034692250093 1105 18-19 -0.5928170199398792 1105 20-21 -0.5466413589006649 1105 22-23 -0.5859254778338467 1105 24-25 -0.5828571515628127 1105 26-27 -0.6056031934162718 1105 28-29 -0.6189365877512074 1105 30-31 -0.6378174491878426 1105 32-33 -0.5523564152700189 1105 34-35 -0.6576151164968564 1105 36-37 -0.6913062177673268 1105 38-39 -0.6351864714222302 1105 40-41 -0.7037746127292266 1105 42-43 -0.721451353828634 1105 44-45 -0.6934729607547112 1105 46-47 -0.6319853936949258 1105 48-49 -0.6524827148404171 1105 50-51 -0.6893681107010039 1105 52-53 -0.5315559968432382 1105 54-55 -0.5650504019489446 1105 56-57 -0.5356456097752869 1105 58-59 -0.47326674639612065 1105 60-61 -0.4811463481706397 1105 62-63 -0.49465401039692125 1105 64-65 -0.47002649772352356 1105 66-67 -0.487297508653981 1105 68-69 -0.4715523065410707 1105 70-71 -0.4882941330858799 1105 72-73 -0.5784023655178174 1105 74-75 -0.6142343288129979 1105 76-77 -0.5788046604021346 1105 78-79 -0.4869552428407715 1105 80-81 -0.4752915420740962 1105 82-83 -0.4534541521750981 1105 84-85 -0.5131933557267416 1105 86-87 -0.517221098557215 1105 88-89 -0.4582363926027213 1105 90-91 -0.5720033659955028 1105 92-93 -0.5906465910274861 1105 94-95 -0.5884319177746242 1105 96-97 -0.6256834705635903 1105 98-99 -0.6812074075162862 1105 100 -0.7143607508297798 1106 1 -0.5274902669729506 1106 2 -0.5974382182678362 1106 3 -0.5684550441033345 1106 4 -0.44498365518052907 1106 5 -0.4088174759287071 1106 6 -0.40129783567802235 1106 7 -0.4770507175842198 1106 8 -0.4273531157519628 1106 9 -0.5943150471227625 1106 10-11 -0.5770648943970471 1106 12-13 -0.5779585041268263 1106 14-15 -0.7619742965753531 1106 16-17 -0.7454994386337717 1106 18-19 -0.7267920109379347 1106 20-21 -0.7270255897917934 1106 22-23 -0.7243969462817361 1106 24-25 -0.8342888132431909 1106 26-27 -0.7980017333000262 1106 28-29 -0.8067263645122225 1106 30-31 -0.7772256593139701 1106 32-33 -0.7505153688501096 1106 34-35 -0.7935056721778082 1106 36-37 -0.9060551279615936 1106 38-39 -0.8616618037666655 1106 40-41 -0.8907896002864675 1106 42-43 -0.8588253149668503 1106 44-45 -0.8367754802883454 1106 46-47 -0.8320429091411725 1106 48-49 -0.8120452163691212 1106 50-51 -0.7103454842090784 1106 52-53 -0.6892608696905498 1106 54-55 -0.8143654709545771 1106 56-57 -0.6271072957467254 1106 58-59 -0.6287500296601607 1106 60-61 -0.7046826443080079 1106 62-63 -0.6546407051122998 1106 64-65 -0.6395367242340697 1106 66-67 -0.6780574698018995 1106 68-69 -0.7286743388271191 1106 70-71 -0.7251541873132581 1106 72-73 -0.6317145361495236 1106 74-75 -0.6972822586439449 1106 76-77 -0.6387736208442405 1106 78-79 -0.6944291986020765 1106 80-81 -0.7539705658925868 1106 82-83 -0.6113362452006825 1106 84-85 -0.5618714396171427 1106 86-87 -0.6011927470440313 1106 88-89 -0.6736127299555292 1106 90-91 -0.6487684647805771 1106 92-93 -0.6258452339808187 1106 94-95 -0.7259355866677062 1106 96-97 -0.6924711152384653 1106 98-99 -0.5833625571095951 1106 100 -0.6298008926069549 1107 1 -0.4534643753502223 1107 2 -0.4779084399328646 1107 3 -0.4461729348855208 1107 4 -0.29398878216923663 1107 5 -0.2766380564544306 1107 6 -0.2570901163647008 1107 7 -0.2963975996020096 1107 8 -0.30502089795410114 1107 9 -0.43193797705342973 1107 10-11 -0.4513261079295887 1107 12-13 -0.4428880003616982 1107 14-15 -0.5976579633501373 1107 16-17 -0.6161678695230535 1107 18-19 -0.6328738341752924 1107 20-21 -0.6043971253837483 1107 22-23 -0.6446135648075852 1107 24-25 -0.6173465421721218 1107 26-27 -0.6713637167911344 1107 28-29 -0.6317347013017667 1107 30-31 -0.6013698395219436 1107 32-33 -0.6557643533684114 1107 34-35 -0.668928702025184 1107 36-37 -0.7477862421638051 1107 38-39 -0.689847127651035 1107 40-41 -0.6844240206645082 1107 42-43 -0.7447357230663485 1107 44-45 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0.5630973228169438 2311 10-11 0.5706412558625971 2311 12-13 0.5987254832937836 2311 14-15 0.7863717966069572 2311 16-17 0.7053850953704668 2311 18-19 0.6393403123556354 2311 20-21 0.6860340704869046 2311 22-23 0.7237596696079223 2311 24-25 0.7422138422987601 2311 26-27 0.7418239617951414 2311 28-29 0.7515649472309391 2311 30-31 0.796366285750409 2311 32-33 0.8217826724591717 2311 34-35 0.8787243860099494 2311 36-37 0.860475652546306 2311 38-39 0.8703842127433816 2311 40-41 0.8692107763203794 2311 42-43 0.9057669465779483 2311 44-45 0.9303209059655373 2311 46-47 0.9500516658381812 2311 48-49 0.9858569886313262 2311 50-51 0.9325214945628773 2311 52-53 0.8332698722925969 2311 54-55 0.84126997639504 2311 56-57 0.7257635243308158 2311 58-59 0.7193034313578579 2311 60-61 0.78509247440482 2311 62-63 0.7993867732363356 2311 64-65 0.7622953996053923 2311 66-67 0.7504101051174175 2311 68-69 0.7425790462878652 2311 70-71 0.7163816822343421 2311 72-73 0.6799724543261192 2311 74-75 0.6986792240220367 2311 76-77 0.7336765616246197 2311 78-79 0.6884949535999496 2311 80-81 0.7443689709163905 2311 82-83 0.724420933622433 2311 84-85 0.7708274576413245 2311 86-87 0.7331349643098122 2311 88-89 0.7675638615029357 2311 90-91 0.7725304503535462 2311 92-93 0.7914924757053718 2311 94-95 0.8320976060734644 2311 96-97 0.8300251957741374 2311 98-99 0.8953517954028065 2311 100 0.9391175921993629 2312 1 0.5424907362324589 2312 2 0.5487128428427184 2312 3 0.5724778660167296 2312 4 0.44775161009890496 2312 5 0.5110068244429584 2312 6 0.48827009380975284 2312 7 0.46792309269487475 2312 8 0.5038627155139537 2312 9 0.6219091883131256 2312 10-11 0.6492634742792234 2312 12-13 0.6311560650316963 2312 14-15 0.8167504314858647 2312 16-17 0.8216179059076509 2312 18-19 0.7775730825923048 2312 20-21 0.8022597580842685 2312 22-23 0.8149497688873808 2312 24-25 0.8473910713430044 2312 26-27 0.8600040484070774 2312 28-29 0.879513599352137 2312 30-31 0.9022356710836661 2312 32-33 0.9037390171543862 2312 34-35 0.9037071364355569 2312 36-37 0.9891406186519163 2312 38-39 1.0282345391499064 2312 40-41 1.0060412319106504 2312 42-43 0.9923028642093286 2312 44-45 0.9615267691776594 2312 46-47 0.9781105338329468 2312 48-49 0.961411184756443 2312 50-51 0.9765500564712255 2312 52-53 0.8417656928228539 2312 54-55 0.8378927230398361 2312 56-57 0.8108811729355665 2312 58-59 0.8481254915773775 2312 60-61 0.7982209067635964 2312 62-63 0.8047958483189532 2312 64-65 0.7988962302080083 2312 66-67 0.7727951306929128 2312 68-69 0.7968915127491201 2312 70-71 0.8041833977799584 2312 72-73 0.8233030007935369 2312 74-75 0.7747885019287892 2312 76-77 0.8393130506941588 2312 78-79 0.8629376344363138 2312 80-81 0.8548395366928858 2312 82-83 0.8554766950104238 2312 84-85 0.8699187313189469 2312 86-87 0.8488544583665174 2312 88-89 0.8367048440043234 2312 90-91 0.8542471024703175 2312 92-93 0.9059290868422529 2312 94-95 0.9508853912592663 2312 96-97 0.881774609896631 2312 98-99 0.9359565096281983 2312 100 1.0570119949053058 2313 1 0.43935671448800306 2313 2 0.5032447289043134 2313 3 0.5124915857859058 2313 4 0.42624879690210093 2313 5 0.4231651394849507 2313 6 0.4129031024822538 2313 7 0.451531352570818 2313 8 0.4226612411699264 2313 9 0.5239633899095182 2313 10-11 0.5848766485070698 2313 12-13 0.5865998985069041 2313 14-15 0.7457957384975487 2313 16-17 0.7404438667757915 2313 18-19 0.6693704805084408 2313 20-21 0.6769067956076995 2313 22-23 0.668132688519826 2313 24-25 0.687906601932319 2313 26-27 0.7250096818187046 2313 28-29 0.739246252282598 2313 30-31 0.805644508812712 2313 32-33 0.7981253593553888 2313 34-35 0.8235838911655264 2313 36-37 0.8410401279521764 2313 38-39 0.8467687686569008 2313 40-41 0.8744145946966384 2313 42-43 0.7579440475896533 2313 44-45 0.8091916660988971 2313 46-47 0.8909692579444055 2313 48-49 0.9517578355719678 2313 50-51 0.8487839970420623 2313 52-53 0.7234459506749147 2313 54-55 0.6728050171249222 2313 56-57 0.6878488953303474 2313 58-59 0.6734193583906318 2313 60-61 0.6394132446892655 2313 62-63 0.6142235221537717 2313 64-65 0.6299725817184836 2313 66-67 0.6078222248576282 2313 68-69 0.6422327565566093 2313 70-71 0.6312194906942565 2313 72-73 0.6643524544999408 2313 74-75 0.6793535842365941 2313 76-77 0.717793507185327 2313 78-79 0.701240346323516 2313 80-81 0.6575171509104862 2313 82-83 0.6211561170438813 2313 84-85 0.7026486822595501 2313 86-87 0.7560789416604052 2313 88-89 0.7411919468588977 2313 90-91 0.747991739505224 2313 92-93 0.7420187997697099 2313 94-95 0.850039151359038 2313 96-97 0.8675241862791694 2313 98-99 0.8585450962819152 2313 100 0.8605476835377814 2314 1 0.36750237584408296 2314 2 0.2828315925942455 2314 3 0.34437516113893096 2314 4 0.23690325960289016 2314 5 0.24308279507555852 2314 6 0.29419032715039606 2314 7 0.3334775371858143 2314 8 0.3508794595708764 2314 9 0.4127596233599178 2314 10-11 0.4511232218692456 2314 12-13 0.48738111292560404 2314 14-15 0.5606318195025537 2314 16-17 0.5813924399650077 2314 18-19 0.5369812784349577 2314 20-21 0.5656592827839191 2314 22-23 0.6152604068045164 2314 24-25 0.6309137576980035 2314 26-27 0.632438670414075 2314 28-29 0.5728434922752967 2314 30-31 0.6168939485008451 2314 32-33 0.7031015141786625 2314 34-35 0.6970633969810649 2314 36-37 0.6630671698798238 2314 38-39 0.7241262733509899 2314 40-41 0.6715995380864754 2314 42-43 0.5675668772627702 2314 44-45 0.718147625539963 2314 46-47 0.5733706619554866 2314 48-49 0.4672644838071207 2314 50-51 0.6446048541592546 2314 52-53 0.5989336133036502 2314 54-55 0.6005109800232944 2314 56-57 0.605400398385953 2314 58-59 0.5825449095521975 2314 60-61 0.36521058392009564 2314 62-63 0.5586674364800359 2314 64-65 0.5674013269204465 2314 66-67 0.5700082727371623 2314 68-69 0.6285128601241823 2314 70-71 0.5854275595908192 2314 72-73 0.6508386357479949 2314 74-75 0.6558843304456943 2314 76-77 0.6427860717016287 2314 78-79 0.629990780328967 2314 80-81 0.6458421738346445 2314 82-83 0.6353020671530274 2314 84-85 0.6745577890260819 2314 86-87 0.6823910164249583 2314 88-89 0.5456727223766222 2314 90-91 0.586607907943776 2314 92-93 0.7244818450008665 2314 94-95 0.7423883751461737 2314 96-97 0.7677380590017577 2314 98-99 0.765458579519759 2314 100 0.7349409287073918 2315 1 0.3528367519453077 2315 2 0.4181374817903851 2315 3 0.407977788167063 2315 4 0.2909817409954769 2315 5 0.3024872672661374 2315 6 0.3068472594205076 2315 7 0.2778238202197585 2315 8 0.3155668892154253 2315 9 0.2196568367074221 2315 10-11 0.4123810309572704 2315 12-13 0.3854431083334404 2315 14-15 0.5385217733577292 2315 16-17 0.3444128399775508 2315 18-19 0.5085729875027099 2315 20-21 0.3984426957995808 2315 22-23 0.3321735188996442 2315 24-25 0.49300950504889585 2315 26-27 0.5818016894274507 2315 28-29 0.5019505023108906 2315 30-31 0.5715073276506715 2315 32-33 0.5764189039347443 2315 34-35 0.5020254392776025 2315 36-37 0.6634753126579014 2315 38-39 0.7258521441787309 2315 40-41 0.6832522250224358 2315 42-43 0.676971100571663 2315 44-45 0.6287078409026989 2315 46-47 0.6729347912841845 2315 48-49 0.5738778854645119 2315 50-51 0.5555991593752694 2315 52-53 0.27193531596355314 2315 54-55 0.47811674036364593 2315 56-57 0.4711491028253718 2315 58-59 0.46134524015364775 2315 60-61 0.53260523724893 2315 62-63 0.5483541890427333 2315 64-65 0.5830802470991756 2315 66-67 0.48834910158305433 2315 68-69 0.5698971341901 2315 70-71 0.4869715875649945 2315 72-73 0.5140300112957448 2315 74-75 0.20599860578601437 2315 76-77 0.4517956413106887 2315 78-79 0.49661382468314486 2315 80-81 0.5213671596081966 2315 82-83 0.44188057281544246 2315 84-85 0.5010151628896011 2315 86-87 0.4491251485788439 2315 88-89 0.35136314552681114 2315 90-91 0.5487039536435248 2315 92-93 0.6555709735210833 2315 94-95 0.6166169269398125 2315 96-97 0.5726358403560106 2315 98-99 0.6825283958722821 2315 100 0.6996942733526303 2316 1 0.13806699578240256 2316 2 0.2013030447958073 2316 3 0.19436277119509526 2316 4 0.05527587314949045 2316 5 0.09113448404045954 2316 6 -0.0646448263872017 2316 7 -0.008899936101002481 2316 8 0.039077027453600977 2316 9 0.1553514567198775 2316 10-11 0.12172751222163214 2316 12-13 0.1395132274646187 2316 14-15 -0.07105107133453004 2316 16-17 0.16460793449047628 2316 18-19 0.21470798509628253 2316 20-21 0.22777933163045816 2316 22-23 0.15515132400476261 2316 24-25 0.16179952449203228 2316 26-27 0.10084399304601277 2316 28-29 0.15881981007925106 2316 30-31 -0.4266429188181817 2316 32-33 -0.28783582812281594 2316 34-35 -0.4399680842235938 2316 36-37 -0.1850265313145414 2316 38-39 -0.09776930684034824 2316 40-41 0.14630713785452798 2316 42-43 0.17873731232871393 2316 44-45 0.014369504825154422 2316 46-47 -0.1421720279654295 2316 48-49 0.01714341164525024 2316 50-51 0.2002872097068007 2316 52-53 -0.23588059005410145 2316 54-55 -0.16045505150151484 2316 56-57 -0.17215756322464415 2316 58-59 -0.5300165699719628 2316 60-61 -0.049902912606320626 2316 62-63 0.0023917955859147355 2316 64-65 -0.3197796701909681 2316 66-67 -0.7029531967968907 2316 68-69 -0.802827661574895 2316 70-71 -0.48950439647416744 2316 72-73 -0.7231769163488835 2316 74-75 -0.5175664411137859 2316 76-77 -0.17510651548473533 2316 78-79 -0.08051515927210318 2316 80-81 -0.18819087989752603 2316 82-83 -0.014862785049412963 2316 84-85 -0.03211995409160551 2316 86-87 0.1059204693182565 2316 88-89 0.10250835331481767 2316 90-91 -0.46292315191729827 2316 92-93 -0.5270813549485212 2316 94-95 -0.22604216703630442 2316 96-97 0.0272414329257451 2316 98-99 0.17050020887614892 2316 100 -0.12653192724000206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 291.0 1 711.0 2 425.0 3 389.0 4 358.0 5 369.0 6 404.0 7 837.0 8 2171.0 9 3700.0 10 5323.0 11 7577.0 12 10504.0 13 13716.0 14 17220.0 15 21191.0 16 26974.0 17 35656.0 18 45373.0 19 57695.0 20 74191.0 21 97541.0 22 107982.0 23 100530.0 24 91628.0 25 90457.0 26 97415.0 27 111265.0 28 131082.0 29 156893.0 30 193621.0 31 240601.0 32 305105.0 33 402814.0 34 569877.0 35 799900.0 36 1207678.0 37 1884086.0 38 2093775.0 39 573460.0 40 255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.528945162304005 22.69360023301871 14.138149192525445 21.639305412151842 2 14.428049562907653 21.765234491737772 42.52049621423481 21.28621973111976 3 17.039816848405852 30.677150844829633 30.03890541221479 22.244126894549723 4 10.946041506469244 18.68380927843984 42.7778217381891 27.59232747690182 5 11.170364969736791 40.222774047269255 36.13023864502001 12.476622337973946 6 25.828211512149156 41.7720068972942 19.75055837153184 12.649223219024803 7 25.312686166227483 33.748949898474955 25.149567350339975 15.788796584957588 8 24.81632693122327 35.76212772679968 21.71515140061189 17.70639394136516 9 26.53286448381361 16.252758601140076 23.06016713074472 34.154209784301585 10-11 22.95134233294664 28.216465377936405 29.497150665396386 19.335041623720564 12-13 24.1212927834466 26.498005332477998 29.45664730469298 19.92405457938242 14-15 22.343365003132178 27.408869283775317 27.269842347045316 22.977923366047193 16-17 19.637656550553636 30.287649202508724 28.716545839401235 21.358148407536408 18-19 19.301060712861606 29.46658846890715 31.626443093745326 19.60590772448592 20-21 20.554071374990855 28.65881111158508 31.31919933507133 19.467918178352736 22-23 20.967831116586154 28.376156484576143 30.774008951084497 19.882003447753206 24-25 20.31409152698807 28.949057506611613 31.441519486472036 19.295331479928283 26-27 19.877341092926986 29.13380103453474 31.132278280770166 19.856579591768106 28-29 19.530559114315047 28.256499894294528 32.02435554255101 20.18858544883941 30-31 20.237481680638783 28.566596689913524 31.420707351203504 19.77521427824419 32-33 20.249874330327355 29.15712353344484 30.88175836045299 19.711243775774815 34-35 20.407231465302953 28.802696324256445 31.34434135396586 19.44573085647474 36-37 20.426274650418325 28.35838358035873 30.870099648972154 20.34524212025079 38-39 20.118897126866834 28.95627782719265 31.396868422058382 19.527956623882133 40-41 19.891176203673837 28.100257914256172 32.25881591697619 19.7497499650938 42-43 19.93787921818922 28.066559591759816 31.293328172266428 20.702233017784533 44-45 19.720366876702727 28.975733871678997 31.055995813377614 20.247903438240662 46-47 20.08534400208206 28.624261119769535 30.59161801546109 20.69877686268731 48-49 20.326297497738693 28.64548058900794 30.496409533818497 20.53181237943487 50-51 20.435463535808267 28.384908904397626 29.982751344819697 21.196876214974406 52-53 19.997333647071063 29.045513015233148 30.07639837443334 20.88075496326245 54-55 20.34479400782308 29.132979033071997 29.756684639264048 20.765542319840876 56-57 20.53026078710091 28.913311628009463 29.477239982165244 21.07918760272438 58-59 19.91891175987296 28.626203931770338 30.47142114427371 20.98346316408299 60-61 20.316117722459133 29.734180734544914 29.340006030489214 20.60969551250674 62-63 20.275363360887113 28.994969891563887 29.96079582578227 20.76887092176673 64-65 19.812681646363455 28.64901342015512 29.956452127389888 21.581852806091533 66-67 20.284315547860377 28.61961386719688 29.4008028421112 21.695267742831543 68-69 19.79122592240708 28.93689025210115 29.718766978689455 21.553116846802318 70-71 20.31639825404692 28.3203663387239 30.074104994355068 21.289130412874112 72-73 20.56391454280669 28.758501231807543 29.53030062746233 21.14728359792344 74-75 20.427006443656254 29.151678373782186 29.338210761495954 21.083104421065602 76-77 20.15668474372089 28.91042480590106 29.8410178566431 21.09187259373495 78-79 20.06147848519335 28.706513183159117 29.550816251015526 21.681192080632 80-81 19.925473625351103 29.500220861630332 29.322085082093214 21.252220430925348 82-83 21.043187442577665 28.622319307751127 29.784487808973665 20.550005440697543 84-85 20.513394361872013 28.960122095369673 29.086037644853924 21.44044589790439 86-87 19.90644301213253 29.416501899973273 29.829522995853758 20.847532092040435 88-89 20.846179883839785 28.47983770865407 29.58011793151642 21.093864475989722 90-91 20.687291464967366 28.660585402888465 29.814848072937984 20.83727505920618 92-93 21.03143062895152 28.970619209435043 29.29418554593933 20.703764615674103 94-95 20.099335776861135 28.26136799257123 29.8857743020121 21.753521928555536 96-97 20.86712504142743 28.40814433216896 29.40526976737239 21.31946085903122 98-99 20.302003940839562 28.791277045742923 29.422311737060365 21.484407276357146 100 20.97124860667804 28.194649769073806 28.796362115520996 22.037739508727164 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4344.0 1 2911.0 2 2963.5 3 3792.0 4 2493.0 5 2041.0 6 2994.5 7 5148.5 8 8216.0 9 10003.0 10 8942.5 11 7806.5 12 8375.5 13 9427.5 14 10619.5 15 11923.5 16 13100.0 17 14210.0 18 15378.5 19 16467.5 20 17744.5 21 19202.5 22 20919.5 23 23761.5 24 25956.5 25 29153.5 26 38208.5 27 49046.0 28 54686.5 29 69831.5 30 105083.0 31 140380.5 32 193008.5 33 267374.0 34 341630.5 35 405045.5 36 481495.5 37 559072.5 38 565069.5 39 535697.5 40 482023.0 41 419082.0 42 375033.0 43 354510.0 44 357266.0 45 372593.0 46 363196.0 47 329088.5 48 311745.0 49 290205.0 50 264343.5 51 240253.5 52 217706.0 53 198643.5 54 181627.0 55 154481.0 56 131674.0 57 104445.5 58 77820.5 59 69882.5 60 56006.0 61 38030.5 62 27194.0 63 21659.5 64 15625.5 65 7657.5 66 4196.0 67 2918.5 68 2283.5 69 1813.0 70 1370.5 71 1098.0 72 896.0 73 779.5 74 665.0 75 515.0 76 374.5 77 248.5 78 173.0 79 141.5 80 105.5 81 71.5 82 56.0 83 44.5 84 32.5 85 20.0 86 16.5 87 14.0 88 14.5 89 13.0 90 8.0 91 7.0 92 8.0 93 6.5 94 1.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02515384551155198 2 0.11460133764184263 3 0.017252824328047894 4 0.017889498426058133 5 0.020008266325993836 6 0.04729131701777677 7 0.04785493015372026 8 0.040006095371692425 9 0.034119469285171546 10-11 0.037871671551313844 12-13 0.03657223015455525 14-15 0.03172933209755934 16-17 0.03822653908135234 18-19 0.037709893706737475 20-21 0.04878906674014512 22-23 0.048622070255421125 24-25 0.06690818533269875 26-27 0.058704483020632414 28-29 0.05117920392775732 30-31 0.049728421966717604 32-33 0.045360420163155564 34-35 0.0464145854729758 36-37 0.04197874134749464 38-39 0.04723913061630053 40-41 0.05612125614755809 42-43 0.057410260264021434 44-45 0.06032226146639614 46-47 0.06167388926463098 48-49 0.060755408598649 50-51 0.05449304042149912 52-53 0.06433017709977205 54-55 0.05101220744303332 56-57 0.05589685462121022 58-59 0.05401814416806526 60-61 0.049081310588412114 62-63 0.05637696951479171 64-65 0.05486356387198049 66-67 0.05281263829396392 68-69 0.058631422058565676 70-71 0.05886104222506116 72-73 0.061407738617102116 74-75 0.05171672386296268 76-77 0.05214465235506792 78-79 0.062446248006479466 80-81 0.06304639162345634 82-83 0.05283351285455441 84-85 0.05818261900586993 86-87 0.05614213070814859 88-89 0.06430408389903393 90-91 0.06950706812621595 92-93 0.0742560306605546 94-95 0.0777577381996109 96-97 0.07378113440712072 98-99 0.08300769018812154 100 0.1271469485567329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9581040.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.6814262518915 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39243864900571 28.002427767960942 2 15.726095713998895 12.166156231824704 3 5.632718120940465 6.536447117785545 4 2.5435436300064755 3.935515813702555 5 1.2702539003282864 2.4567616283363067 6 0.7120264552908322 1.6525319291836826 7 0.4344759036267798 1.176430333705424 8 0.28099538914364786 0.8695441937825253 9 0.18571502651567232 0.6465349891830646 >10 0.6371639073702408 4.084579238628048 >50 0.05242606523793507 1.451100834178785 >100 0.08423101153632671 7.59818402412489 >500 0.023292610636864785 6.28709957058271 >1k 0.022722178804897707 16.264935407053002 >5k 0.001663757862283301 4.280371001735591 >10k+ 2.3767969461190014E-4 2.5913799182321897 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54150 0.5651787279877758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38970 0.40674081310588417 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23694 0.24730091931564843 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 13321 0.13903501081302239 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 10796 0.11268087806751668 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.0437280295249784E-5 0.0 9 0.0 0.0 0.0 4.174912118099914E-5 0.0 10-11 0.0 0.0 0.0 7.306096206674849E-5 0.0 12-13 0.0 0.0 0.0 8.349824236199827E-5 0.0 14-15 0.0 0.0 0.0 8.871688250962317E-5 0.0 16-17 0.0 0.0 0.0 1.1481008324774764E-4 0.0 18-19 0.0 0.0 0.0 1.409032839858721E-4 0.0 20-21 0.0 0.0 0.0 2.609320073812446E-4 0.0 22-23 0.0 0.0 0.0 8.402010637676077E-4 0.0 24-25 0.0 0.0 0.0 0.002030051017426083 0.0 26-27 0.0 0.0 0.0 0.004101851156033166 0.0 28-29 0.0 0.0 0.0 0.008730784966976445 0.0 30-31 0.0 0.0 0.0 0.024005744679074507 0.0 32-33 0.0 0.0 0.0 0.055719420856190985 0.0 34-35 0.0 0.0 0.0 0.10425277422910248 0.0 36-37 0.0 0.0 0.0 0.180846755675793 0.0 38-39 0.0 0.0 0.0 0.2954533119577833 0.0 40-41 0.0 0.0 0.0 0.4208415787847666 0.0 42-43 0.0 0.0 0.0 0.5448834364536627 0.0 44-45 0.0 0.0 0.0 0.6955403588754456 0.0 46-47 0.0 0.0 0.0 0.8760322470212002 0.0 48-49 0.0 0.0 0.0 1.0726079841019347 0.0 50-51 0.0 0.0 0.0 1.2794853168340805 0.0 52-53 0.0 0.0 0.0 1.4807056436462012 0.0 54-55 0.0 0.0 0.0 1.695264814675651 0.0 56-57 0.0 0.0 0.0 1.9479722451842387 0.0 58-59 0.0 0.0 0.0 2.2401534697694614 0.0 60-61 0.0 0.0 0.0 2.5420048345482327 0.0 62-63 0.0 0.0 0.0 2.845823626662659 0.0 64-65 0.0 0.0 0.0 3.158127927657123 0.0 66-67 0.0 0.0 0.0 3.4996983625994673 0.0 68-69 0.0 0.0 0.0 3.866960162988569 0.0 70-71 0.0 0.0 0.0 4.274003657223016 0.0 72-73 0.0 0.0 0.0 4.68076534488949 0.0 74-75 0.0 0.0 0.0 5.07874927982766 0.0 76-77 0.0 0.0 0.0 5.502513297095096 0.0 78-79 0.0 1.5655920442874675E-5 0.0 5.960949959503353 0.0 80-81 0.0 2.087456059049957E-5 0.0 6.461114868532017 0.0 82-83 0.0 2.087456059049957E-5 0.0 6.962511376635522 0.0 84-85 0.0 2.087456059049957E-5 0.0 7.441384233861877 0.0 86-87 0.0 2.087456059049957E-5 0.0 7.93423782804372 0.0 88 0.0 2.087456059049957E-5 0.0 8.317353857201306 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20670 0.0 55.660103 1 GTATCAA 41660 0.0 42.9825 1 GCATCGT 4140 0.0 35.640182 8 ATCAACG 50275 0.0 34.47891 3 CAACGCA 50435 0.0 34.36075 5 TATCAAC 54405 0.0 32.315464 2 AACGCAG 54585 0.0 32.25711 6 TACCTGG 2855 0.0 31.959908 2 TCAACGC 54945 0.0 31.676853 4 TAGGCAT 6475 0.0 31.641018 5 GTACTAG 5815 0.0 29.685503 1 ACCTGGG 3060 0.0 29.636904 3 ACGCAGA 60115 0.0 28.852928 7 CGCAGAG 60540 0.0 28.587086 8 CATGGGT 5375 0.0 28.324728 4 GTACATG 58295 0.0 28.062496 1 TACCCCG 585 0.0 27.31032 5 CTGTGCG 5215 0.0 27.303846 9 ATGGGTA 3900 0.0 27.229998 5 ACATGGG 58975 0.0 26.556099 3 >>END_MODULE