FastQCFastQC Report
Mon 6 Jun 2016
ERR1211286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581040
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468730.48922663927924315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364050.3799691891485684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222870.23261566594023195No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG131810.13757379157168742No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT108530.11327580304434591No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209500.055.973791
GTATCAA419150.042.732191
TACCTGG30600.036.028372
ATCAACG514300.033.927783
CAACGCA516250.033.747495
ACCTGGG32450.033.255153
TATCAAC557350.031.6825262
AACGCAG560300.031.5546156
TCAACGC557150.031.394214
TAGGCAT64100.030.1507995
GTACTAG55650.028.669241
ACGCAGA624900.027.8722347
CGCAGAG627900.027.754018
GCATCGT53100.026.9440388
GTACATG604500.026.369491
CTGTGCG53700.026.293169
CATGGGT53700.025.7746934
GCAGAGT671300.025.7426669
GCTATTG96550.025.653058
ACATGGG611000.025.0412123
Produced by FastQC (version 0.11.5)