FastQCFastQC Report
Mon 6 Jun 2016
ERR1211285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9391074
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271260.28884875148465444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209140.2227008327269064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112420.11970941768747644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155550.045.901981
GTATCAA286600.041.7021261
CAACGCA347800.033.4913145
ATCAACG352600.033.088693
TATCAAC364350.032.3492472
TCAACGC365650.031.9077594
AACGCAG373950.031.5130146
ACGCAGA420350.027.744887
CGCAGAG431300.026.930828
GTACATG420050.026.6617161
TACATGG440050.025.1383762
GTACTAG33000.025.0858271
ACATGGG442400.024.2692473
GCAGAGT467450.024.1967039
TACCTGG60200.023.047692
CATGGGG299650.022.188474
TCTAACG17450.021.83192
GAGTACT223600.021.27883312-13
GTGGTAT88500.020.674551
CTAACGC18650.020.659523
Produced by FastQC (version 0.11.5)