##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211285_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9391074 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41626910830433 31.0 31.0 34.0 28.0 34.0 2 31.431488560307372 31.0 31.0 34.0 28.0 34.0 3 31.481744899465173 33.0 31.0 34.0 28.0 34.0 4 35.09244874441411 37.0 35.0 37.0 32.0 37.0 5 35.13533617134739 37.0 35.0 37.0 32.0 37.0 6 35.19629288407268 37.0 35.0 37.0 32.0 37.0 7 35.13170261463172 37.0 35.0 37.0 32.0 37.0 8 35.13100514382061 37.0 35.0 37.0 32.0 37.0 9 36.689152806164664 39.0 37.0 39.0 32.0 39.0 10-11 36.62267755530411 39.0 37.0 39.0 32.0 39.0 12-13 36.544344981202364 39.0 37.0 39.0 32.0 39.0 14-15 37.68353060576458 40.0 37.0 41.0 32.0 41.0 16-17 37.681427811132146 40.0 37.0 41.0 32.0 41.0 18-19 37.67790414600077 40.0 37.0 41.0 32.0 41.0 20-21 37.64106517529305 40.0 37.0 41.0 32.0 41.0 22-23 37.52017229339264 40.0 37.0 41.0 31.5 41.0 24-25 37.40750807628606 40.0 37.0 41.0 31.0 41.0 26-27 37.266061208760576 40.0 36.5 41.0 31.0 41.0 28-29 37.13276213136005 40.0 36.0 41.0 30.5 41.0 30-31 36.95464389908971 39.0 36.0 41.0 30.0 41.0 32-33 36.84269227353549 39.0 36.0 41.0 30.0 41.0 34-35 36.70495099921479 39.0 35.5 41.0 30.0 41.0 36-37 36.6148182305879 39.0 35.0 41.0 30.0 41.0 38-39 36.46835463121683 39.0 35.0 41.0 29.5 41.0 40-41 36.30147435745901 39.0 35.0 40.0 29.0 41.0 42-43 36.15320819535657 39.0 35.0 40.0 28.0 41.0 44-45 36.01757637092413 39.0 35.0 40.0 28.0 41.0 46-47 35.855978879519 39.0 35.0 40.0 27.0 41.0 48-49 35.62180965670167 38.0 34.0 40.0 26.5 41.0 50-51 34.939932642421944 37.5 33.5 39.5 25.5 40.5 52-53 35.17809246311977 38.0 34.0 40.0 26.0 40.5 54-55 35.534056434865704 38.0 34.0 40.0 27.0 41.0 56-57 35.42752735203663 38.0 34.0 40.0 26.0 41.0 58-59 35.20084310910552 38.0 34.0 40.0 26.0 41.0 60-61 34.931226769164 37.5 34.0 40.0 26.0 41.0 62-63 34.650803305351445 37.0 33.5 40.0 26.0 41.0 64-65 34.369365633792256 36.5 33.0 40.0 26.0 41.0 66-67 34.14078996715392 36.0 33.0 39.0 25.0 41.0 68-69 33.80055161954852 35.5 33.0 39.0 25.0 41.0 70-71 33.45969635634859 35.0 32.0 38.5 24.0 40.5 72-73 33.09831069375026 35.0 32.0 37.5 24.0 40.0 74-75 32.72871207276186 35.0 32.0 37.0 23.0 39.0 76-77 32.383468120898634 35.0 31.0 36.5 23.0 39.0 78-79 32.02573039036856 35.0 31.0 36.0 22.0 39.0 80-81 31.695759505249345 35.0 31.0 36.0 20.5 37.5 82-83 31.388740521052224 34.0 30.5 35.0 20.0 37.0 84-85 31.08994173616351 34.0 30.0 35.0 20.0 36.5 86-87 30.846994710083216 34.0 30.0 35.0 19.5 36.0 88-89 30.592629234952256 34.0 30.0 35.0 19.0 36.0 90-91 30.3431061239641 34.0 29.0 35.0 18.0 35.5 92-93 30.107548188844003 34.0 29.0 35.0 18.0 35.0 94-95 29.88788103469316 34.0 29.0 35.0 17.0 35.0 96-97 29.687381070578297 34.0 29.0 35.0 16.5 35.0 98-99 29.448941729135562 34.0 28.0 35.0 15.0 35.0 100 27.732638141281818 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3322524041269972 1101 2 -0.4223899383941898 1101 3 -0.34908526049768085 1101 4 -0.2554837745616254 1101 5 -0.31546540065413353 1101 6 -0.3202418932518256 1101 7 -0.33824497006303034 1101 8 -0.5086842586625124 1101 9 -0.5190888479072058 1101 10-11 -0.5320096081777592 1101 12-13 -0.46792245551068135 1101 14-15 -0.5814835946359551 1101 16-17 -0.5967582150991646 1101 18-19 -0.6518623761652762 1101 20-21 -0.73219756291995 1101 22-23 -0.6998379016011 1101 24-25 -0.6957211368834209 1101 26-27 -0.7514861255866521 1101 28-29 -0.7861759843967349 1101 30-31 -0.7564446090941388 1101 32-33 -0.7574831986351 1101 34-35 -0.7251557861896032 1101 36-37 -0.7999449055693191 1101 38-39 -0.8934138747867806 1101 40-41 -0.909812161737257 1101 42-43 -0.8841333715002264 1101 44-45 -0.9360897649328948 1101 46-47 -0.9158409374958794 1101 48-49 -0.8510815331555364 1101 50-51 -0.894124349543624 1101 52-53 -0.7151133035660209 1101 54-55 -0.6536879262011368 1101 56-57 -0.6141213291296808 1101 58-59 -0.6441545033609444 1101 60-61 -0.6263969539707688 1101 62-63 -0.6744175398373287 1101 64-65 -0.7089635855144252 1101 66-67 -0.7520923145963891 1101 68-69 -0.7412245481264961 1101 70-71 -0.7501986302334203 1101 72-73 -0.8852997342650255 1101 74-75 -0.8616174671276511 1101 76-77 -0.8788676616717979 1101 78-79 -0.8359491777961203 1101 80-81 -0.9388994423597588 1101 82-83 -1.0292828715034226 1101 84-85 -1.0540997030061803 1101 86-87 -1.0776373690130328 1101 88-89 -1.0163866559745784 1101 90-91 -1.060929159730044 1101 92-93 -1.0982972420824453 1101 94-95 -1.1839208868876447 1101 96-97 -1.2626813678755973 1101 98-99 -1.2348579548250278 1101 100 -1.2442820526353877 1102 1 -0.3142836298123619 1102 2 -0.34447240028203296 1102 3 -0.4182371724942229 1102 4 -0.23613041556262715 1102 5 -0.20706960737067703 1102 6 -0.23912665589575255 1102 7 -0.33766144574848056 1102 8 -0.29160947138208115 1102 9 -0.4643337574289035 1102 10-11 -0.3443294072864518 1102 12-13 -0.37742296042048906 1102 14-15 -0.4815258983987789 1102 16-17 -0.5265087690891974 1102 18-19 -0.5508617587721076 1102 20-21 -0.5798298041110002 1102 22-23 -0.5829664001489334 1102 24-25 -0.6119963014704481 1102 26-27 -0.604670947665312 1102 28-29 -0.6704713700370064 1102 30-31 -0.6765186855055063 1102 32-33 -0.7156444998970173 1102 34-35 -0.6793764271951304 1102 36-37 -0.8315309927170631 1102 38-39 -0.8641014836423082 1102 40-41 -0.9003365550135811 1102 42-43 -0.8771647466608599 1102 44-45 -0.8405397853242036 1102 46-47 -0.8626308376199034 1102 48-49 -0.892606191136629 1102 50-51 -0.8698165799634197 1102 52-53 -0.7234257250793945 1102 54-55 -0.6159263862095585 1102 56-57 -0.6366266051867058 1102 58-59 -0.7529540306400193 1102 60-61 -0.8127435619213657 1102 62-63 -0.779810339415441 1102 64-65 -0.7969735581425681 1102 66-67 -0.6804608146432898 1102 68-69 -0.714474478638806 1102 70-71 -0.7849487227670195 1102 72-73 -0.8304803252674091 1102 74-75 -0.8291342052886534 1102 76-77 -0.887339317962244 1102 78-79 -0.838046166138664 1102 80-81 -0.9041133540704891 1102 82-83 -0.955609507598016 1102 84-85 -0.9674464365246251 1102 86-87 -0.9991529179410392 1102 88-89 -0.9996169818575851 1102 90-91 -1.1251430167336878 1102 92-93 -1.0459922052720039 1102 94-95 -1.0817483209195657 1102 96-97 -1.0092863650449324 1102 98-99 -1.008800718827377 1102 100 -1.1023189114276732 1103 1 -0.5222414093649306 1103 2 -0.5258115639530345 1103 3 -0.5574297028050985 1103 4 -0.4347389315600765 1103 5 -0.3646897128054647 1103 6 -0.5095478526973949 1103 7 -0.4461121219733073 1103 8 -0.40447405432001204 1103 9 -0.6366646277626202 1103 10-11 -0.6663778671120184 1103 12-13 -0.741065321931174 1103 14-15 -0.815737418014514 1103 16-17 -0.8142299443665095 1103 18-19 -0.8019376746468865 1103 20-21 -0.889722276970879 1103 22-23 -0.8557660185113463 1103 24-25 -0.9081857913406637 1103 26-27 -0.8851448536673061 1103 28-29 -0.9478103763035293 1103 30-31 -0.9876936744470513 1103 32-33 -1.0138419482513328 1103 34-35 -1.0080044297797954 1103 36-37 -1.0768611262208552 1103 38-39 -1.0908438111433512 1103 40-41 -1.1213707854632489 1103 42-43 -1.1399268937999665 1103 44-45 -1.1408419451244995 1103 46-47 -1.0905250797236121 1103 48-49 -1.158258897868329 1103 50-51 -1.1934885397425816 1103 52-53 -1.0708450753549883 1103 54-55 -1.0243643723126326 1103 56-57 -1.0020327625419228 1103 58-59 -0.9899719705452625 1103 60-61 -0.9049265370375252 1103 62-63 -1.0239668892577924 1103 64-65 -1.124470837289806 1103 66-67 -1.0673344279668981 1103 68-69 -1.0349077402043605 1103 70-71 -0.9770875295962753 1103 72-73 -1.1325905202004662 1103 74-75 -0.9815173484376523 1103 76-77 -0.9480234009994994 1103 78-79 -0.9915690444319587 1103 80-81 -1.0395140858320104 1103 82-83 -1.1639058132842877 1103 84-85 -1.1751272153239753 1103 86-87 -1.221806729617228 1103 88-89 -1.2834848332050584 1103 90-91 -1.255428512522574 1103 92-93 -1.2587991023627367 1103 94-95 -1.4504037174546056 1103 96-97 -1.5784627529310775 1103 98-99 -1.6211011416075074 1103 100 -1.5612409572686445 1104 1 -0.3178885026877545 1104 2 -0.3356888624516614 1104 3 -0.41756949867961524 1104 4 -0.253889583705714 1104 5 -0.15916592916189387 1104 6 -0.18858734661159815 1104 7 -0.23052140673971167 1104 8 -0.28319322686656534 1104 9 -0.3695773791832906 1104 10-11 -0.34977504970377993 1104 12-13 -0.3370543323183952 1104 14-15 -0.41807413698797546 1104 16-17 -0.4338359078035623 1104 18-19 -0.49367626574794343 1104 20-21 -0.519076443733411 1104 22-23 -0.5011939700059855 1104 24-25 -0.575677346346815 1104 26-27 -0.5802905796797901 1104 28-29 -0.5074376289264393 1104 30-31 -0.6091679683962639 1104 32-33 -0.666485048940217 1104 34-35 -0.6817810389410894 1104 36-37 -0.727169503351746 1104 38-39 -0.7304317009332308 1104 40-41 -0.7242608924839828 1104 42-43 -0.7571672067575435 1104 44-45 -0.7452298919969778 1104 46-47 -0.829489934335065 1104 48-49 -0.7194006614135517 1104 50-51 -0.5859954075105307 1104 52-53 -0.5022245403796646 1104 54-55 -0.5068211585161251 1104 56-57 -0.5021373906652329 1104 58-59 -0.4567865832082063 1104 60-61 -0.5215967437838316 1104 62-63 -0.5543007633043189 1104 64-65 -0.5152265295185288 1104 66-67 -0.4916939325667258 1104 68-69 -0.41979704550672636 1104 70-71 -0.6117203439520509 1104 72-73 -0.6038792230192129 1104 74-75 -0.5824772509203697 1104 76-77 -0.5938292099109255 1104 78-79 -0.582764550180439 1104 80-81 -0.6584907197186958 1104 82-83 -0.7736997147187274 1104 84-85 -0.8042688562110314 1104 86-87 -0.765734247085458 1104 88-89 -0.7250992591412526 1104 90-91 -0.6654349519422276 1104 92-93 -0.7495292807222036 1104 94-95 -0.788567073009844 1104 96-97 -0.8481018526437545 1104 98-99 -0.8866608747530478 1104 100 -0.9518669744024137 1105 1 -0.40660902773921137 1105 2 -0.386988658210651 1105 3 -0.4321941932366009 1105 4 -0.3278456187393033 1105 5 -0.31075327837871924 1105 6 -0.36096058898635874 1105 7 -0.3532731702976264 1105 8 -0.3137518321126578 1105 9 -0.4684097628305679 1105 10-11 -0.5270047722688815 1105 12-13 -0.5814249304512842 1105 14-15 -0.6205248526159934 1105 16-17 -0.6412101902764178 1105 18-19 -0.6524300216395957 1105 20-21 -0.6882375023035507 1105 22-23 -0.6915998266146985 1105 24-25 -0.7024461897407264 1105 26-27 -0.6314494882344377 1105 28-29 -0.7129377801361727 1105 30-31 -0.7666323098978012 1105 32-33 -0.7339296841455791 1105 34-35 -0.810986810616086 1105 36-37 -0.726811941027897 1105 38-39 -0.7646265244479977 1105 40-41 -0.858034747568702 1105 42-43 -0.8118321938390096 1105 44-45 -0.8607367937114319 1105 46-47 -0.8407377160365783 1105 48-49 -0.8893991460475021 1105 50-51 -0.8700913334508371 1105 52-53 -0.7331929939623265 1105 54-55 -0.727619185062359 1105 56-57 -0.7685370574154504 1105 58-59 -0.677956292831901 1105 60-61 -0.7097303488632178 1105 62-63 -0.6864814835856592 1105 64-65 -0.6348995700057429 1105 66-67 -0.7196903532171817 1105 68-69 -0.7372591028456128 1105 70-71 -0.6815032386725548 1105 72-73 -0.6983781108137421 1105 74-75 -0.7917162246710632 1105 76-77 -0.7438540192905592 1105 78-79 -0.6884378636688844 1105 80-81 -0.7718319554441493 1105 82-83 -0.7560120848780727 1105 84-85 -0.7301498049217088 1105 86-87 -0.7769176812971921 1105 88-89 -0.815052427923348 1105 90-91 -0.7294386471342555 1105 92-93 -0.7303911879888609 1105 94-95 -0.8327303510225086 1105 96-97 -0.9562144544096078 1105 98-99 -1.0411834318071023 1105 100 -1.0272328751620172 1106 1 -0.5038374614728944 1106 2 -0.5192270984936016 1106 3 -0.49783128557082534 1106 4 -0.3927171513460408 1106 5 -0.4088226797339729 1106 6 -0.41461721953203323 1106 7 -0.3899598935506532 1106 8 -0.40773668269282837 1106 9 -0.5939864766641278 1106 10-11 -0.5816839103749203 1106 12-13 -0.5840397448408865 1106 14-15 -0.7218577328061642 1106 16-17 -0.6860956955223614 1106 18-19 -0.7280727499963575 1106 20-21 -0.701534079724496 1106 22-23 -0.7832150036927885 1106 24-25 -0.7794122092215332 1106 26-27 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0.7908353395754872 2311 16-17 0.8119057461772883 2311 18-19 0.6801776624754723 2311 20-21 0.7646906580024009 2311 22-23 0.787453326451228 2311 24-25 0.8215370181419601 2311 26-27 0.8739059302912224 2311 28-29 0.8674265749650942 2311 30-31 0.9162553472973158 2311 32-33 0.9222839157880287 2311 34-35 0.9430961778612357 2311 36-37 0.9788765297305844 2311 38-39 1.0077260123291936 2311 40-41 1.0306638449692684 2311 42-43 1.0318219081680553 2311 44-45 1.0693070333300554 2311 46-47 1.047679271712859 2311 48-49 1.0485269459869144 2311 50-51 1.0188721361622086 2311 52-53 0.9965426887142499 2311 54-55 0.8981576702658316 2311 56-57 0.8861704323642172 2311 58-59 0.8379704894908357 2311 60-61 0.9005797794863213 2311 62-63 0.8216985195783479 2311 64-65 0.8879156803955937 2311 66-67 0.8851803938589313 2311 68-69 0.8755963286472692 2311 70-71 0.8370578127269184 2311 72-73 0.8546355466943183 2311 74-75 0.8138820050440998 2311 76-77 0.814939635935275 2311 78-79 0.8446119594150154 2311 80-81 0.8403201664684588 2311 82-83 0.8586529113225332 2311 84-85 0.9418022824981023 2311 86-87 0.9633573793677819 2311 88-89 1.0169221965243551 2311 90-91 1.047651614656619 2311 92-93 1.0208856617201967 2311 94-95 1.0452062379762737 2311 96-97 1.066264696462266 2311 98-99 1.1143610163642172 2311 100 1.192090962132223 2312 1 0.4989104439665475 2312 2 0.4957819491224491 2312 3 0.5262986315540097 2312 4 0.39191651950305584 2312 5 0.36894701182664136 2312 6 0.4518226797493483 2312 7 0.4737817390052257 2312 8 0.4819297395062492 2312 9 0.5900500971055749 2312 10-11 0.6059919006385428 2312 12-13 0.612124394780416 2312 14-15 0.7998277923755595 2312 16-17 0.7853467258484699 2312 18-19 0.7722584967167307 2312 20-21 0.7920654780199499 2312 22-23 0.8220541501331837 2312 24-25 0.8687257638303123 2312 26-27 0.8607901059084995 2312 28-29 0.8847336227768707 2312 30-31 0.9279496761719557 2312 32-33 0.8811570092037329 2312 34-35 0.9093089516155999 2312 36-37 0.9414363554905307 2312 38-39 0.9826083212633918 2312 40-41 0.9639771608768228 2312 42-43 1.0121902570870986 2312 44-45 0.9890800339599224 2312 46-47 0.9670147027650273 2312 48-49 0.9310007345089844 2312 50-51 0.9706903229487338 2312 52-53 0.8503808991707658 2312 54-55 0.8545600881556155 2312 56-57 0.804530364472285 2312 58-59 0.7431525970690487 2312 60-61 0.7795430248462907 2312 62-63 0.7817858585721282 2312 64-65 0.8017729815940413 2312 66-67 0.8056227398973945 2312 68-69 0.8142315942626368 2312 70-71 0.8015944431778834 2312 72-73 0.8055537538170725 2312 74-75 0.7912033085301644 2312 76-77 0.8064258629228149 2312 78-79 0.7733600555003797 2312 80-81 0.8043924677276735 2312 82-83 0.8173580447890991 2312 84-85 0.9013620651837861 2312 86-87 0.966171390539909 2312 88-89 0.922766939382889 2312 90-91 0.8789039647745547 2312 92-93 0.9697175268133442 2312 94-95 1.0354599541738594 2312 96-97 0.9672940346352554 2312 98-99 1.0554930879045905 2312 100 1.2742813234648551 2313 1 0.4883299811070252 2313 2 0.4957152241492402 2313 3 0.5705970679920398 2313 4 0.42721038664679867 2313 5 0.39618981637519113 2313 6 0.4386499630671139 2313 7 0.4804597018530785 2313 8 0.4493054406166266 2313 9 0.5628299517202819 2313 10-11 0.5911861300897812 2313 12-13 0.5982340430407547 2313 14-15 0.7722570562180593 2313 16-17 0.7295574747406448 2313 18-19 0.6963443218843182 2313 20-21 0.7623079048778223 2313 22-23 0.7057116442415179 2313 24-25 0.751955011139593 2313 26-27 0.7815007405370125 2313 28-29 0.7970477279693569 2313 30-31 0.8705011822149444 2313 32-33 0.862033870998431 2313 34-35 0.9258234963503114 2313 36-37 0.937898053135541 2313 38-39 0.9503900654701383 2313 40-41 1.002632383944892 2313 42-43 0.8142578544950325 2313 44-45 0.9212202238118294 2313 46-47 0.953535916581103 2313 48-49 0.9602130644186602 2313 50-51 0.9051671393795644 2313 52-53 0.8010234344737981 2313 54-55 0.7319256222474664 2313 56-57 0.6712542331907869 2313 58-59 0.7445712428731994 2313 60-61 0.7651878755969861 2313 62-63 0.8037002868683416 2313 64-65 0.7957258316328506 2313 66-67 0.7852639941037154 2313 68-69 0.7513234944771483 2313 70-71 0.7154462401804764 2313 72-73 0.7672160436974522 2313 74-75 0.7719228086986263 2313 76-77 0.819081973455809 2313 78-79 0.8533471864620346 2313 80-81 0.8276037630586686 2313 82-83 0.794462952957435 2313 84-85 0.8522388210839082 2313 86-87 0.8702065889880615 2313 88-89 0.8840028612390682 2313 90-91 0.9374792681767659 2313 92-93 1.0105018572390563 2313 94-95 1.0700587884908543 2313 96-97 1.143439253227296 2313 98-99 1.0424660616715435 2313 100 1.169849908482096 2314 1 0.339977791673558 2314 2 0.2637187596980759 2314 3 0.30209507584902795 2314 4 0.25288602950009675 2314 5 0.20094332835690665 2314 6 0.25433777665408286 2314 7 0.3038026207514051 2314 8 0.3254455000057632 2314 9 0.41963968282426123 2314 10-11 0.43295682274184344 2314 12-13 0.43508236409994083 2314 14-15 0.49957701064213467 2314 16-17 0.5494775701826669 2314 18-19 0.5502106580822641 2314 20-21 0.5554577970462304 2314 22-23 0.5977646192776831 2314 24-25 0.6200325005488239 2314 26-27 0.6937536825792137 2314 28-29 0.6674933220723887 2314 30-31 0.7022883141350178 2314 32-33 0.733463181905492 2314 34-35 0.7043627666257279 2314 36-37 0.6970721254086811 2314 38-39 0.7370907132773326 2314 40-41 0.7914684712644444 2314 42-43 0.6742842869430135 2314 44-45 0.7939903054021542 2314 46-47 0.6446515154648935 2314 48-49 0.6117737788486579 2314 50-51 0.756762465009821 2314 52-53 0.6490646942469382 2314 54-55 0.6155628211174005 2314 56-57 0.6131591802657752 2314 58-59 0.5851493911998276 2314 60-61 0.4258732735542452 2314 62-63 0.5977178337909592 2314 64-65 0.6143437836213437 2314 66-67 0.624823345488501 2314 68-69 0.6226736571383711 2314 70-71 0.6370129738450956 2314 72-73 0.676951482344073 2314 74-75 0.7687048748904814 2314 76-77 0.7588068417685179 2314 78-79 0.7475113124489674 2314 80-81 0.7540431489091617 2314 82-83 0.7879048910307382 2314 84-85 0.8427534464051902 2314 86-87 0.8660098682324531 2314 88-89 0.6792979323925294 2314 90-91 0.7163829570561653 2314 92-93 0.8865502333202571 2314 94-95 0.9283805727333956 2314 96-97 0.9447528074951528 2314 98-99 0.9179987266606169 2314 100 0.9531215103920907 2315 1 0.2836626574356842 2315 2 0.3293930335213453 2315 3 0.3061218168860904 2315 4 0.21390383681066538 2315 5 0.2608559500164205 2315 6 0.24099674509134417 2315 7 0.22995771601699744 2315 8 0.23269529895825514 2315 9 0.1443406117668573 2315 10-11 0.3368846681655171 2315 12-13 0.3148996170692584 2315 14-15 0.4736998163030819 2315 16-17 0.3274275127896189 2315 18-19 0.4984517372434354 2315 20-21 0.42446715692470605 2315 22-23 0.3504870431201965 2315 24-25 0.48617147335070143 2315 26-27 0.5257917630394573 2315 28-29 0.4374617816704287 2315 30-31 0.4346174366399751 2315 32-33 0.5362235255420771 2315 34-35 0.4537013847591851 2315 36-37 0.5576629774033464 2315 38-39 0.649319217518233 2315 40-41 0.6937145499788357 2315 42-43 0.713665210971115 2315 44-45 0.683758954706569 2315 46-47 0.7373216454756104 2315 48-49 0.6035900755535479 2315 50-51 0.6020363535779509 2315 52-53 0.3335102327105872 2315 54-55 0.5193920673438939 2315 56-57 0.4995358659718079 2315 58-59 0.4854537419022975 2315 60-61 0.5630196833956091 2315 62-63 0.6030603620407078 2315 64-65 0.5844491703198997 2315 66-67 0.49320648963097113 2315 68-69 0.5761698131787298 2315 70-71 0.5288366508837541 2315 72-73 0.5541185382180984 2315 74-75 0.3244204579626242 2315 76-77 0.5297579425465884 2315 78-79 0.6056590076197033 2315 80-81 0.6499891805406435 2315 82-83 0.5157070078719883 2315 84-85 0.6181955146513687 2315 86-87 0.6105845109864951 2315 88-89 0.5381470685755794 2315 90-91 0.6832386513402291 2315 92-93 0.8131065644266009 2315 94-95 0.6864136708239599 2315 96-97 0.7364183400476669 2315 98-99 0.8746737445315063 2315 100 0.8499662157973447 2316 1 0.14881170371059582 2316 2 0.13264361088855026 2316 3 0.13646759569029854 2316 4 0.05898680661649536 2316 5 0.05781750163733079 2316 6 -0.12038444556813488 2316 7 -0.08844853665600283 2316 8 -0.01857277049688122 2316 9 0.0359950895702994 2316 10-11 0.06641561077093883 2316 12-13 0.04429284690417745 2316 14-15 -0.06165686832611783 2316 16-17 0.108720708410182 2316 18-19 0.19079039518543794 2316 20-21 0.21522094188750174 2316 22-23 0.13969583620890091 2316 24-25 0.13092267085304599 2316 26-27 0.054430352552756744 2316 28-29 0.15822786241629672 2316 30-31 -0.42101050365598525 2316 32-33 -0.2418668858855142 2316 34-35 -0.4041749731229487 2316 36-37 -0.2030115799484662 2316 38-39 -0.01778507210626401 2316 40-41 0.17337240644962293 2316 42-43 0.19411790962818998 2316 44-45 0.06300196214818499 2316 46-47 -0.03589845973352368 2316 48-49 0.09548223618993035 2316 50-51 0.24753214093574627 2316 52-53 -0.12461503458904133 2316 54-55 -0.06270623476884651 2316 56-57 -0.09628485081888272 2316 58-59 -0.4619324953742421 2316 60-61 0.026700613170646648 2316 62-63 -0.004456852055604088 2316 64-65 -0.22162430500299024 2316 66-67 -0.5406423043130886 2316 68-69 -0.6019431583378179 2316 70-71 -0.3609638366707699 2316 72-73 -0.5239097527749763 2316 74-75 -0.3441460675698309 2316 76-77 -0.032527896988092664 2316 78-79 0.004805252594469067 2316 80-81 -0.11830266788977895 2316 82-83 0.11560971080586668 2316 84-85 0.12712203932846933 2316 86-87 0.17754777332504545 2316 88-89 0.21425101605655072 2316 90-91 -0.3766291957216126 2316 92-93 -0.43788511720884316 2316 94-95 -0.010110043999407736 2316 96-97 0.28961887892372573 2316 98-99 0.32903543300982463 2316 100 0.1063895328788611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 273.0 1 670.0 2 420.0 3 384.0 4 338.0 5 370.0 6 419.0 7 1139.0 8 2994.0 9 4912.0 10 7444.0 11 11208.0 12 16252.0 13 21132.0 14 24461.0 15 28357.0 16 33303.0 17 40368.0 18 48281.0 19 57372.0 20 68791.0 21 83338.0 22 92812.0 23 95931.0 24 97133.0 25 102613.0 26 113498.0 27 130068.0 28 153575.0 29 183793.0 30 223856.0 31 276680.0 32 349154.0 33 454860.0 34 628182.0 35 875606.0 36 1299354.0 37 1853253.0 38 1646124.0 39 362199.0 40 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.048950568531914 19.27260081449366 13.32448577565157 23.35396284132285 2 15.872903346990677 20.217458557316387 40.132855806047964 23.776782289644974 3 18.951373143513017 26.263416533529526 29.112585083606145 25.67262523935131 4 13.006419365152327 16.682606623936223 38.12630452929803 32.18466948161342 5 13.837858678443373 37.41852168805393 34.142018104930635 14.601601528572061 6 30.65313003239912 36.76536827307132 18.32517709144323 14.256324603086332 7 27.912099572359427 32.056753969293105 22.25547404119118 17.77567241715629 8 26.352844225375126 34.46927574856012 20.26646600029039 18.911414025774366 9 26.83492368272234 16.183376635162904 20.61410661442681 36.36759306768794 10-11 25.156383862362496 26.0114571868135 27.78580583927182 21.04635311155218 12-13 25.894423216836554 23.65635988482765 27.920319951596085 22.528896946739714 14-15 23.512334764222793 25.019588628156924 25.54648437916085 25.921592228459435 16-17 22.676907520937835 27.916454903060295 26.334515516648693 23.07212205935318 18-19 22.454820880574925 27.14792393012254 28.34005434080075 22.057200848501783 20-21 23.43243247130363 26.282815779390624 28.152024988628625 22.13272676067712 22-23 23.52105061778 26.026821239552937 27.842764652649134 22.609363490017927 24-25 23.10105137949371 26.266090505937523 28.10263612873438 22.53022198583438 26-27 22.871083675856703 26.45467349550492 27.904565674827243 22.769677153811134 28-29 22.678347049537077 26.40038239027374 28.207062436835606 22.714208123353572 30-31 23.164421592471918 26.28252830877351 28.0115403124158 22.541509786338768 32-33 23.06428350337298 26.551544632057972 27.744837887819905 22.639333976749143 34-35 23.20916081263562 26.514047890085244 27.893143809760666 22.383647487518466 36-37 23.18312370665023 26.31598430778666 27.680964836865417 22.81992714869769 38-39 23.113491544563658 26.43225197320148 27.859290459785573 22.594966022449288 40-41 23.035747495109764 26.08713672013029 28.169241578532993 22.707874206226954 42-43 22.93578954243394 26.1444958241185 27.812147501678886 23.107567131768675 44-45 22.840737360999512 26.506291364920582 27.737602383947014 22.915368890132896 46-47 22.98404950360174 26.36748393610766 27.40757804541623 23.240888514874367 48-49 23.15813727629013 26.34810077522477 27.392090379226968 23.101671569258126 50-51 23.054850118774766 26.440777972586766 27.244372155819534 23.25999975281893 52-53 22.92752109038959 26.650900209139095 27.175061221999908 23.246517478471407 54-55 22.89035491660598 26.585662074201043 27.234731187796214 23.289251821396764 56-57 23.100644358976925 26.54222427545041 26.985789752606127 23.371341612966535 58-59 22.79587696481853 26.434608453838365 27.43602019167054 23.333494389672563 60-61 23.003061733098505 26.710626243927287 27.12530285332942 23.161009169644796 62-63 22.99680971811239 26.544875598397606 27.152129768602013 23.306184914887993 64-65 22.72944934798744 26.40330568175105 27.246756984052833 23.620487986208673 66-67 22.93595932209746 26.336578832080704 27.05154351187199 23.67591833394985 68-69 22.64333841791744 26.484441337780662 27.201409782762553 23.670810461539347 70-71 22.813709542602908 26.22877834911882 27.238930049807568 23.7185820584707 72-73 22.92812193101914 26.51382100896503 26.979893217446417 23.578163842569413 74-75 22.939263423307786 26.687525026862673 26.908605746255564 23.464605803573974 76-77 22.844263287134385 26.60128219770741 26.915929261995856 23.638525253162346 78-79 22.7928289149191 26.45571763434277 26.958222727800162 23.793230722937974 80-81 22.79014023923044 26.866088940038104 26.818327454026708 23.525443366704753 82-83 23.174146806372676 26.488754287149852 27.070117456838975 23.2669814496385 84-85 23.14787447660762 26.597826455243386 26.685238763245835 23.569060304903168 86-87 22.807568494106672 26.786936890575426 27.01221582028316 23.393278795034746 88-89 22.991940270199137 26.359088453510044 26.967025859542904 23.68194541674791 90-91 23.124057247428624 26.418746216801964 26.958385234508064 23.49881130126135 92-93 23.240679752057527 26.520518915753076 26.799750979091858 23.43905035309754 94-95 22.892253247237996 26.323260772738394 26.99752553076883 23.786960449254785 96-97 23.1152312082582 26.2564086278932 26.897929450132775 23.730430713715826 98-99 22.983218590795595 26.401185269686263 26.787115158494757 23.828480981023386 100 23.3261451243418 26.20426846334573 26.3791205364021 24.090465875910365 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1772.0 1 1286.0 2 1713.5 3 2304.0 4 1603.5 5 1281.5 6 1748.5 7 3028.5 8 4884.5 9 5988.0 10 5435.0 11 4781.0 12 5046.0 13 5562.5 14 6300.5 15 7060.0 16 7651.5 17 8351.0 18 8957.0 19 9626.0 20 10476.0 21 11388.0 22 12615.5 23 14518.0 24 16841.5 25 19855.0 26 24501.0 27 29963.5 28 36174.5 29 46287.0 30 62537.5 31 80211.0 32 103957.5 33 134729.5 34 165900.5 35 195933.0 36 232970.5 37 273684.5 38 292218.5 39 295711.5 40 292069.5 41 285276.0 42 288215.0 43 301448.5 44 318000.5 45 340799.5 46 360659.0 47 370865.0 48 383336.5 49 389366.0 50 383311.0 51 375324.0 52 366075.5 53 356989.5 54 347560.0 55 325044.0 56 296120.0 57 260102.0 58 225453.5 59 199635.0 60 169117.0 61 137375.0 62 111014.0 63 88311.0 64 66639.5 65 48475.0 66 35708.0 67 27022.0 68 20566.5 69 15517.5 70 12157.0 71 9526.5 72 7548.0 73 5946.0 74 4553.0 75 3442.0 76 2516.0 77 1713.0 78 1162.0 79 850.0 80 621.0 81 428.0 82 308.5 83 222.0 84 168.5 85 127.0 86 99.5 87 73.0 88 55.0 89 42.0 90 33.0 91 25.5 92 20.0 93 21.0 94 16.5 95 11.0 96 10.0 97 8.0 98 2.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025758502169187465 2 0.1121064534258808 3 0.01738885243583428 4 0.017804140399702954 5 0.020434297504204526 6 0.04683170423318994 7 0.04741736674633807 8 0.03923938838092427 9 0.032956826876244405 10-11 0.037445131408825015 12-13 0.036247185359203855 14-15 0.03166836934731853 16-17 0.03877085837040577 18-19 0.036934007760986654 20-21 0.04860466438662926 22-23 0.04813613437611076 24-25 0.06717549025808976 26-27 0.059450069289199506 28-29 0.05166608206899446 30-31 0.04976001679893056 32-33 0.04466475293454188 34-35 0.04587867159815799 36-37 0.04121466831163294 38-39 0.04644303729264619 40-41 0.05626087069487473 42-43 0.05740557469784606 44-45 0.05995586873237289 46-47 0.06194712127707651 48-49 0.06103135807469945 50-51 0.05581896170768115 52-53 0.06571665817988442 54-55 0.05259781788536647 56-57 0.05656435036077875 58-59 0.05456777361141016 60-61 0.05029776147009383 62-63 0.05732038742320634 64-65 0.05508954566857848 66-67 0.052667032546011246 68-69 0.05872065325009685 70-71 0.05913061700930053 72-73 0.06211217162169098 74-75 0.05227836560546749 76-77 0.053263343468489335 78-79 0.06383721393314545 80-81 0.0627936698188088 82-83 0.05356682313439336 84-85 0.06035518408224661 86-87 0.05724584855789657 88-89 0.06542382692331038 90-91 0.07083854306759801 92-93 0.07584861965734697 94-95 0.07928273166626096 96-97 0.07501804372960963 98-99 0.0840478948414207 100 0.13085830225595071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9391074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.82004176052648 #Duplication Level Percentage of deduplicated Percentage of total 1 72.53121259350121 37.58570465536857 2 14.996992655021318 15.542895713310273 3 5.460969659263104 8.489630273879463 4 2.4700496153561406 5.119922968733103 5 1.321560828375218 3.4241668657739885 6 0.7676511004678142 2.386782725025374 7 0.49632132358722114 1.8003574200450707 8 0.3582107719237641 1.4849997728127906 9 0.2584464205410809 1.205343387476766 >10 1.2085771358642694 10.96181546427845 >50 0.07399522276623591 2.644255568215261 >100 0.049298687884616985 5.124019862895026 >500 0.004754421187464854 1.629745269645932 >1k 0.0018310682433181236 1.5273987816723293 >5k 3.212400425591612E-5 0.08611323643160364 >10k+ 9.637201276774837E-5 0.9868480344361737 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27126 0.28884875148465444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20914 0.2227008327269064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11242 0.11970941768747644 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.194522798989764E-5 2 0.0 0.0 0.0 0.0 3.194522798989764E-5 3 0.0 0.0 0.0 0.0 3.194522798989764E-5 4 0.0 0.0 0.0 0.0 6.389045597979528E-5 5 0.0 0.0 0.0 0.0 6.389045597979528E-5 6 2.129681865993176E-5 0.0 0.0 0.0 7.453886530976116E-5 7 2.129681865993176E-5 0.0 0.0 1.064840932996588E-5 7.453886530976116E-5 8 2.129681865993176E-5 0.0 0.0 1.064840932996588E-5 9.583568396969293E-5 9 2.129681865993176E-5 0.0 0.0 2.129681865993176E-5 9.583568396969293E-5 10-11 2.129681865993176E-5 0.0 0.0 2.66210233249147E-5 9.583568396969293E-5 12-13 2.129681865993176E-5 0.0 0.0 3.194522798989764E-5 1.064840932996588E-4 14-15 2.129681865993176E-5 0.0 0.0 3.194522798989764E-5 1.9699557260436878E-4 16-17 2.129681865993176E-5 0.0 0.0 4.259363731986352E-5 2.555618239191811E-4 18-19 2.129681865993176E-5 0.0 0.0 6.921466064477823E-5 2.981554612390446E-4 20-21 2.129681865993176E-5 0.0 0.0 1.8102295860941995E-4 3.194522798989764E-4 22-23 2.129681865993176E-5 0.0 0.0 5.643656944881916E-4 3.726943265488058E-4 24-25 2.129681865993176E-5 0.0 0.0 0.0016292066274847796 4.152879638686693E-4 26-27 2.129681865993176E-5 0.0 0.0 0.003545920306878638 4.259363731986352E-4 28-29 2.129681865993176E-5 0.0 0.0 0.007965010178814479 4.259363731986352E-4 30-31 2.129681865993176E-5 0.0 0.0 0.020237301931600154 4.3126057786361816E-4 32-33 2.129681865993176E-5 0.0 0.0 0.049392646677046737 4.685300105184987E-4 34-35 2.129681865993176E-5 0.0 0.0 0.09233235730013414 4.791784198484646E-4 36-37 2.129681865993176E-5 0.0 0.0 0.1607856566778198 4.791784198484646E-4 38-39 2.129681865993176E-5 0.0 0.0 0.2609605674494738 5.004752385083964E-4 40-41 3.194522798989764E-5 0.0 0.0 0.3705114026361628 5.217720571683281E-4 42-43 3.194522798989764E-5 0.0 0.0 0.48016872191615145 5.32420466498294E-4 44-45 3.194522798989764E-5 0.0 0.0 0.6147752642562501 5.537172851582258E-4 46-47 3.194522798989764E-5 0.0 0.0 0.775252117063501 5.750141038181575E-4 48-49 3.194522798989764E-5 0.0 0.0 0.9478521838929179 6.495529691279187E-4 50-51 3.194522798989764E-5 0.0 0.0 1.125089632985535 6.602013784578846E-4 52-53 3.194522798989764E-5 0.0 0.0 1.3027689910653457 7.187676297726969E-4 54-55 3.194522798989764E-5 0.0 0.0 1.4959737299482465 8.03954904412424E-4 56-57 3.194522798989764E-5 0.0 0.0 1.724621699285939 8.252517230723557E-4 58-59 3.194522798989764E-5 0.0 0.0 1.979917312971871 8.359001324023216E-4 60-61 3.7269432654880576E-5 0.0 0.0 2.243582576391156 8.83817974387168E-4 62-63 4.259363731986352E-5 0.0 0.0 2.515729297841759 9.477084303669633E-4 64-65 4.259363731986352E-5 0.0 0.0 2.7883179282795556 9.583568396969292E-4 66-67 4.259363731986352E-5 0.0 0.0 3.0933895313784134 9.690052490268951E-4 68-69 4.259363731986352E-5 0.0 0.0 3.4264877478337405 9.79653658356861E-4 70-71 4.259363731986352E-5 0.0 0.0 3.782501341167155 0.0010169230910117414 72-73 4.259363731986352E-5 0.0 0.0 4.1391964326976876 0.0011074345703164516 74-75 4.259363731986352E-5 0.0 0.0 4.490183976827357 0.0011180829796464174 76-77 4.7917841984846464E-5 0.0 0.0 4.872994292239631 0.0011234071843114002 78-79 5.32420466498294E-5 0.0 0.0 5.289118156240702 0.0011287313889763832 80-81 5.32420466498294E-5 0.0 0.0 5.729616229198067 0.0011606766169662808 82-83 5.856625131481234E-5 0.0 0.0 6.178979102922627 0.001240539686941025 84-85 6.389045597979528E-5 1.064840932996588E-5 0.0 6.619642226224604 0.001245863891606008 86-87 6.921466064477823E-5 1.064840932996588E-5 0.0 7.076922192285994 0.0012618365056009568 88 7.453886530976116E-5 1.064840932996588E-5 0.0 7.434879120322127 0.0013097543475858032 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15555 0.0 45.90198 1 GTATCAA 28660 0.0 41.702126 1 CAACGCA 34780 0.0 33.491314 5 ATCAACG 35260 0.0 33.08869 3 TATCAAC 36435 0.0 32.349247 2 TCAACGC 36565 0.0 31.907759 4 AACGCAG 37395 0.0 31.513014 6 ACGCAGA 42035 0.0 27.74488 7 CGCAGAG 43130 0.0 26.93082 8 GTACATG 42005 0.0 26.661716 1 TACATGG 44005 0.0 25.138376 2 GTACTAG 3300 0.0 25.085827 1 ACATGGG 44240 0.0 24.269247 3 GCAGAGT 46745 0.0 24.196703 9 TACCTGG 6020 0.0 23.04769 2 CATGGGG 29965 0.0 22.18847 4 TCTAACG 1745 0.0 21.8319 2 GAGTACT 22360 0.0 21.278833 12-13 GTGGTAT 8850 0.0 20.67455 1 CTAACGC 1865 0.0 20.65952 3 >>END_MODULE