FastQCFastQC Report
Mon 6 Jun 2016
ERR1211285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9391074
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245050.2609392706308139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198130.21097693405461398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110290.1174413065001937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163250.045.6802141
GTATCAA289300.040.2147671
CAACGCA353350.032.1827435
ATCAACG357600.031.8329373
TCAACGC371350.030.7299084
TATCAAC376550.030.6772522
AACGCAG379750.030.2542616
ACGCAGA429000.026.5841757
CGCAGAG444200.025.6426758
GTACATG415000.024.7658631
TACATGG434650.023.5756682
GCAGAGT484400.023.328869
ACATGGG434300.022.9265653
CATGGGG292300.021.1416114
GAGTACT235200.020.84002512-13
GTGGTAT93600.020.807971
TAGTACT47850.020.4948834
AGAGTAC445350.019.63196210-11
TACCTGG58500.019.5697382
CAGAGTA478950.019.27321410-11
Produced by FastQC (version 0.11.5)