##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211285_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9391074 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12290756094564 33.0 31.0 34.0 30.0 34.0 2 32.257204766994704 34.0 31.0 34.0 30.0 34.0 3 32.322813024367605 34.0 31.0 34.0 30.0 34.0 4 35.95879512822495 37.0 35.0 37.0 35.0 37.0 5 35.85967015061323 37.0 35.0 37.0 35.0 37.0 6 35.87457398376373 37.0 35.0 37.0 35.0 37.0 7 35.79459005434309 37.0 35.0 37.0 33.0 37.0 8 35.77786747287903 37.0 35.0 37.0 33.0 37.0 9 37.39442506788893 39.0 37.0 39.0 34.0 39.0 10-11 37.34565652448272 39.0 37.0 39.0 34.0 39.0 12-13 37.278708910184285 39.0 37.0 39.0 34.0 39.0 14-15 38.51534009848075 40.0 38.0 41.0 34.0 41.0 16-17 38.49529952591152 40.0 38.0 41.0 34.0 41.0 18-19 38.499789800399824 40.0 38.0 41.0 34.0 41.0 20-21 38.46754332890998 40.0 38.0 41.0 34.0 41.0 22-23 38.356783153875696 40.0 38.0 41.0 34.0 41.0 24-25 38.26204984648188 40.0 38.0 41.0 34.0 41.0 26-27 38.163354425702536 40.0 38.0 41.0 33.0 41.0 28-29 38.066387348241534 40.0 37.5 41.0 33.0 41.0 30-31 38.08130273491615 40.0 37.0 41.0 33.0 41.0 32-33 38.02957425316849 40.0 37.0 41.0 33.0 41.0 34-35 37.90202190931517 40.0 37.0 41.0 33.0 41.0 36-37 37.75842976000402 40.0 37.0 41.0 33.0 41.0 38-39 37.646999427328545 40.0 37.0 41.0 33.0 41.0 40-41 37.5370514597159 40.0 36.5 41.0 32.0 41.0 42-43 37.438554951222834 40.0 36.0 41.0 32.0 41.0 44-45 37.283791289473385 40.0 36.0 41.0 31.5 41.0 46-47 37.049084854405365 39.0 36.0 41.0 31.0 41.0 48-49 35.500582095296025 39.0 35.0 41.0 24.5 41.0 50-51 36.23270378872533 39.0 35.0 40.0 29.0 41.0 52-53 36.32485331283728 39.0 35.0 40.0 30.0 41.0 54-55 36.14973676067295 38.5 35.0 40.0 29.0 41.0 56-57 35.92720108477475 38.0 35.0 40.0 29.0 41.0 58-59 35.68139501403141 38.0 34.0 40.0 28.0 41.0 60-61 35.4193647606227 37.5 34.0 40.0 28.0 41.0 62-63 35.0934613016573 37.0 34.0 40.0 28.0 41.0 64-65 34.7553541266952 36.0 34.0 39.0 27.0 41.0 66-67 34.412042488431034 36.0 33.0 39.0 26.5 40.5 68-69 34.04863969765332 35.5 33.0 38.5 26.0 40.0 70-71 33.69289742578964 35.0 33.0 38.0 26.0 40.0 72-73 33.319963403546815 35.0 32.0 37.0 26.0 39.5 74-75 32.959434352237025 35.0 32.0 37.0 25.5 39.0 76-77 32.03049470167097 34.0 30.5 36.0 24.5 38.0 78-79 32.471868499811634 34.5 32.0 36.0 25.0 38.0 80-81 32.35041641669525 35.0 32.0 35.5 25.0 37.0 82-83 32.094756307957965 34.0 31.5 35.0 25.0 37.0 84-85 31.825139222627783 34.0 31.0 35.0 24.5 36.0 86-87 31.58089756294115 34.0 31.0 35.0 24.0 36.0 88-89 31.36488270670639 34.0 31.0 35.0 24.0 35.5 90-91 31.156101847349944 34.0 31.0 35.0 23.0 35.0 92-93 30.97686265702943 34.0 30.5 35.0 23.0 35.0 94-95 30.794298447653592 34.0 30.0 35.0 21.0 35.0 96-97 30.614085726510087 34.0 30.0 35.0 20.0 35.0 98-99 30.401780509875653 34.0 29.0 35.0 20.0 35.0 100 27.907065475152255 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26131473107366787 1101 2 -0.23354588387342545 1101 3 -0.29296015621689264 1101 4 -0.18142633012303122 1101 5 -0.15493201742471285 1101 6 -0.21519148808110344 1101 7 -0.24600450947401242 1101 8 -0.24472673410694767 1101 9 -0.32887338392403365 1101 10-11 -0.30889860018319126 1101 12-13 -0.3819030060416111 1101 14-15 -0.50980207383585 1101 16-17 -0.46644348889424947 1101 18-19 -0.5139341551284957 1101 20-21 -0.5654904156721514 1101 22-23 -0.5905998167613049 1101 24-25 -0.552777847201682 1101 26-27 -0.6948072647434529 1101 28-29 -0.6858446385416457 1101 30-31 -0.5819702934088653 1101 32-33 -0.5228548612428412 1101 34-35 -0.60179561738817 1101 36-37 -0.6686822380298949 1101 38-39 -0.6381156307197529 1101 40-41 -0.590144421120506 1101 42-43 -0.6043579519831397 1101 44-45 -0.5524478373067581 1101 46-47 -0.5835830593354814 1101 48-49 0.6001841530606598 1101 50-51 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1105 68-69 -0.6737298642823291 1105 70-71 -0.7688524678510618 1105 72-73 -0.6513378730358355 1105 74-75 -0.6393579677168759 1105 76-77 -0.6183463478200899 1105 78-79 -0.46015542043896573 1105 80-81 -0.4603939518603646 1105 82-83 -0.567324162036563 1105 84-85 -0.5974836924987841 1105 86-87 -0.5760892336719721 1105 88-89 -0.5349265972606077 1105 90-91 -0.614779245204371 1105 92-93 -0.5648458512054582 1105 94-95 -0.5401511156757159 1105 96-97 -0.6734249998045065 1105 98-99 -0.6428539964856128 1105 100 -0.7684609902870605 1106 1 -0.31660160881164146 1106 2 -0.29793440431643603 1106 3 -0.3273868084257501 1106 4 -0.22314508690674728 1106 5 -0.2675766528750785 1106 6 -0.3275465084812268 1106 7 -0.3505772505030933 1106 8 -0.3399911309399215 1106 9 -0.4147567945409989 1106 10-11 -0.42631150653109984 1106 12-13 -0.4935468811862833 1106 14-15 -0.5742003363573289 1106 16-17 -0.5459408082342989 1106 18-19 -0.5363385000954963 1106 20-21 -0.5696063644076403 1106 22-23 -0.6086009010969988 1106 24-25 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0.17157915929104206 2311 9 0.4289836526808273 2311 10-11 0.32697588527636157 2311 12-13 0.428932291371801 2311 14-15 0.39070624809833276 2311 16-17 0.40287933149433997 2311 18-19 0.5732007241972283 2311 20-21 0.6111790626286506 2311 22-23 0.6603906725068924 2311 24-25 0.6077751305109516 2311 26-27 0.6711727311097064 2311 28-29 0.7122898376769626 2311 30-31 0.5934604154948175 2311 32-33 0.5944784948361672 2311 34-35 0.6868874265722553 2311 36-37 0.7407452454905936 2311 38-39 0.7957154673980682 2311 40-41 0.7107326569949279 2311 42-43 0.7881185746578865 2311 44-45 0.7994290666200854 2311 46-47 0.8199158288747412 2311 48-49 -6.324127727477897 2311 50-51 -1.1769126086579433 2311 52-53 0.3313850570030823 2311 54-55 0.8152329768568691 2311 56-57 0.905479733428983 2311 58-59 0.9047841544486701 2311 60-61 0.7804575376247342 2311 62-63 0.6861301679843379 2311 64-65 0.721201346141406 2311 66-67 0.8936832908286476 2311 68-69 0.9194693152384517 2311 70-71 0.9141676386876085 2311 72-73 0.38491546926505293 2311 74-75 0.7333574026744643 2311 76-77 0.8111539690123131 2311 78-79 0.769136696874611 2311 80-81 0.8148938555816088 2311 82-83 0.8650309422501365 2311 84-85 0.846685240549732 2311 86-87 0.8226743959565681 2311 88-89 0.877119809393136 2311 90-91 0.9427666393026541 2311 92-93 0.9259998427698832 2311 94-95 0.9577308726831752 2311 96-97 1.0122946441502165 2311 98-99 1.019689681432851 2311 100 1.312185866502098 2312 1 0.03797381305773939 2312 2 0.2056870838856355 2312 3 0.19392184127751477 2312 4 -0.01862187576162455 2312 5 0.18662626159245121 2312 6 0.13638609312782535 2312 7 0.30010332296188125 2312 8 0.341009493939211 2312 9 0.29487252993622803 2312 10-11 0.2512612471760107 2312 12-13 0.3249305928868651 2312 14-15 0.3722783481885301 2312 16-17 0.4746917446042431 2312 18-19 0.3627961215292288 2312 20-21 0.375594139532744 2312 22-23 0.6363619143622472 2312 24-25 0.7257523093511722 2312 26-27 0.6648186845706618 2312 28-29 0.7661165856343715 2312 30-31 0.7738701020400498 2312 32-33 0.7435901160988081 2312 34-35 0.35251803534961823 2312 36-37 0.6843530532430648 2312 38-39 0.7818658345324181 2312 40-41 0.5716997023459314 2312 42-43 0.6173410547903373 2312 44-45 0.793755480122762 2312 46-47 0.8420894680103856 2312 48-49 -4.6696253428610035 2312 50-51 -0.7792886223861899 2312 52-53 0.49963303378929425 2312 54-55 0.754061694616702 2312 56-57 0.8531121529791719 2312 58-59 0.84352555065297 2312 60-61 0.8893636432536098 2312 62-63 0.6884882320070673 2312 64-65 0.8937001375093274 2312 66-67 0.9284101687423174 2312 68-69 0.8387578293253526 2312 70-71 0.8451000365856203 2312 72-73 0.9420320003101281 2312 74-75 0.615608628870632 2312 76-77 0.8477586491348781 2312 78-79 0.5134804991825916 2312 80-81 0.719799751268404 2312 82-83 0.7250360707117451 2312 84-85 0.8222600814644068 2312 86-87 0.9508217247736042 2312 88-89 0.908238332676639 2312 90-91 0.6888841851456391 2312 92-93 0.9202646875871316 2312 94-95 1.0287357018541883 2312 96-97 1.1006419504305711 2312 98-99 1.0655068553270937 2312 100 0.6432283060095507 2313 1 0.019546675532900792 2313 2 0.1943386006433343 2313 3 0.27131427031590505 2313 4 0.02663484971144925 2313 5 0.10168184087623189 2313 6 0.2698952494224258 2313 7 0.18577668554413407 2313 8 0.257221487605122 2313 9 0.4356796447768545 2313 10-11 0.2493895889044282 2313 12-13 0.030513480504595236 2313 14-15 0.13057698206871748 2313 16-17 0.07334996915745506 2313 18-19 0.4666546809826002 2313 20-21 0.45202104909956375 2313 22-23 0.5177474572923089 2313 24-25 0.27282216641284407 2313 26-27 0.4424599886499152 2313 28-29 0.3619884162977698 2313 30-31 0.5727194720071438 2313 32-33 0.4955341072018342 2313 34-35 0.467984975231893 2313 36-37 -0.24718450534482628 2313 38-39 0.2287001501613588 2313 40-41 0.4153618945300295 2313 42-43 0.5243417569966695 2313 44-45 0.5586733224375351 2313 46-47 0.42985580854071515 2313 48-49 -5.192633730508959 2313 50-51 -1.2423024915020804 2313 52-53 0.14759110727762703 2313 54-55 0.30970035907423465 2313 56-57 0.2193115019210623 2313 58-59 0.3043470603248437 2313 60-61 0.690436002387905 2313 62-63 0.7195331075120492 2313 64-65 0.8044513866953267 2313 66-67 0.822400867554336 2313 68-69 0.8637363685927184 2313 70-71 0.7716930583148454 2313 72-73 0.5207724218320422 2313 74-75 0.7142350281166685 2313 76-77 0.7522769773805287 2313 78-79 0.6839710609579086 2313 80-81 -0.24207387231552246 2313 82-83 0.2492057689429359 2313 84-85 0.6146386092769376 2313 86-87 0.7088507259975358 2313 88-89 0.6901026671422841 2313 90-91 0.7459095562349844 2313 92-93 0.8918426956280605 2313 94-95 0.7479363480616392 2313 96-97 0.783306176132232 2313 98-99 0.9620309396833981 2313 100 0.9930122298228383 2314 1 0.04353842367446248 2314 2 0.1764583115084477 2314 3 0.2097778167921831 2314 4 0.09232212221660774 2314 5 0.20252885035310442 2314 6 0.2815456427528318 2314 7 0.10284549624148553 2314 8 0.24844544112883682 2314 9 0.44944200068075446 2314 10-11 0.30097437546328365 2314 12-13 0.21017693242382762 2314 14-15 0.21288475493747683 2314 16-17 0.4307071848802835 2314 18-19 0.4922353760009912 2314 20-21 0.5694593705820026 2314 22-23 0.31672891886731946 2314 24-25 0.43725203090672693 2314 26-27 0.5206182351215745 2314 28-29 0.5980466917714224 2314 30-31 0.4958933990298746 2314 32-33 0.5040791418022152 2314 34-35 0.3975541586745024 2314 36-37 0.4860079002231714 2314 38-39 0.5259603402951001 2314 40-41 0.3130173810034549 2314 42-43 0.2806158712096547 2314 44-45 0.5514217138614086 2314 46-47 0.6559738699479709 2314 48-49 -6.712858797699294 2314 50-51 -1.6374154345158232 2314 52-53 -0.8431993495300887 2314 54-55 -0.4294061008174097 2314 56-57 -0.13042800325600723 2314 58-59 0.3765712324456132 2314 60-61 0.6491236841914017 2314 62-63 0.5703975518474707 2314 64-65 0.6747247063597328 2314 66-67 0.7823800214755181 2314 68-69 0.7437267060738648 2314 70-71 0.5704532474866681 2314 72-73 0.727994613593367 2314 74-75 0.7770812002393583 2314 76-77 0.7311365199956548 2314 78-79 0.5626702870425788 2314 80-81 0.5837733235339684 2314 82-83 0.5859633385573133 2314 84-85 0.6412442936179872 2314 86-87 0.651409540388677 2314 88-89 0.5204262929868513 2314 90-91 0.7218262750859878 2314 92-93 0.8047267795500908 2314 94-95 0.8915903841953252 2314 96-97 0.8441023696096224 2314 98-99 0.9084742706215394 2314 100 -0.5777166626957069 2315 1 0.1565873607002004 2315 2 0.24160893389655058 2315 3 0.302284475416954 2315 4 0.11706054566046475 2315 5 0.23006578638906205 2315 6 0.29026303697091294 2315 7 0.3414172427085518 2315 8 0.24050400437803887 2315 9 0.41768125408997037 2315 10-11 0.19666358608547085 2315 12-13 0.39624812042558943 2315 14-15 0.24068212911954134 2315 16-17 0.37788278956933397 2315 18-19 0.4315659608258642 2315 20-21 0.34416560064909163 2315 22-23 0.42405512016915026 2315 24-25 0.48071311103016967 2315 26-27 0.5299611792131884 2315 28-29 0.590393556289456 2315 30-31 0.5362195041038404 2315 32-33 0.5889626244605779 2315 34-35 0.6662050783379811 2315 36-37 0.5414812683016379 2315 38-39 0.6233680560517314 2315 40-41 0.661695678354441 2315 42-43 0.6983933296598366 2315 44-45 0.6607627892421917 2315 46-47 0.5506108975722412 2315 48-49 -4.983083734635674 2315 50-51 -0.9891103466391655 2315 52-53 0.23129895531291567 2315 54-55 0.22850544515201676 2315 56-57 0.5674701094749395 2315 58-59 0.6698588532459553 2315 60-61 0.53132948466979 2315 62-63 0.35163109860047115 2315 64-65 0.5118377457513006 2315 66-67 -0.2711178829908292 2315 68-69 0.4642314040558304 2315 70-71 0.47553346887949743 2315 72-73 0.5699703607340254 2315 74-75 0.738983401795501 2315 76-77 0.7097681038719905 2315 78-79 0.6445602410250331 2315 80-81 0.5441785330701521 2315 82-83 0.5874604938209771 2315 84-85 0.6239697718361157 2315 86-87 0.5772020885713474 2315 88-89 0.5553641272068575 2315 90-91 0.6941639292224089 2315 92-93 0.6453495714642017 2315 94-95 0.334345001414615 2315 96-97 0.4425008285890577 2315 98-99 0.36869376896504846 2315 100 0.4553907929020049 2316 1 0.17689614523531105 2316 2 0.2294621970904629 2316 3 0.1464374563424471 2316 4 -0.033460367862758744 2316 5 0.15901818567056125 2316 6 0.11142204817611656 2316 7 0.22009293341080394 2316 8 0.29307713755741105 2316 9 0.4257688856013857 2316 10-11 0.3866810140214838 2316 12-13 0.33730160173599444 2316 14-15 0.4896801831782156 2316 16-17 0.4433491739675901 2316 18-19 0.161846197760255 2316 20-21 0.4951339737566087 2316 22-23 0.5479588709175829 2316 24-25 0.6166107031950858 2316 26-27 0.6108352490104636 2316 28-29 0.6614579434248569 2316 30-31 0.5981187214505113 2316 32-33 0.5408129794344845 2316 34-35 0.5231642777221168 2316 36-37 0.44541741576549754 2316 38-39 0.51307798249087 2316 40-41 0.2011334937680047 2316 42-43 0.12171768172553499 2316 44-45 0.40530378231378705 2316 46-47 0.383386857712523 2316 48-49 -4.962356358876544 2316 50-51 -1.0109056378888397 2316 52-53 0.24896810252017332 2316 54-55 0.5628343092354982 2316 56-57 0.4797557351699169 2316 58-59 0.3646315416387367 2316 60-61 0.5180514289003924 2316 62-63 0.472863242985035 2316 64-65 0.429052376663968 2316 66-67 0.558769530404092 2316 68-69 0.2877503502537522 2316 70-71 -0.11720506931226993 2316 72-73 0.0032484165034176726 2316 74-75 0.23617098198538855 2316 76-77 0.24455479876548836 2316 78-79 0.3310302820822528 2316 80-81 0.21790960825619976 2316 82-83 0.16050471587213622 2316 84-85 0.34301326683227273 2316 86-87 0.36945724210825404 2316 88-89 0.22257266368791306 2316 90-91 0.21343858494048362 2316 92-93 0.250850704814642 2316 94-95 0.4616015449096089 2316 96-97 0.5600927796625328 2316 98-99 0.5674621591797404 2316 100 -0.31740002909782206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 9.0 11 13.0 12 40.0 13 198.0 14 767.0 15 2225.0 16 5084.0 17 10423.0 18 17520.0 19 25834.0 20 36566.0 21 52029.0 22 65592.0 23 70521.0 24 74175.0 25 83712.0 26 99675.0 27 122848.0 28 151595.0 29 186617.0 30 229350.0 31 284498.0 32 359326.0 33 468132.0 34 653869.0 35 927114.0 36 1454832.0 37 2134792.0 38 1667610.0 39 206095.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.2560551687173 20.011186360267054 14.10352698419157 23.62923148682407 2 15.623673581524223 20.61555401032277 39.70052891685453 24.060243491298483 3 18.583230514012836 26.17782916939488 29.713837689830626 25.525102626761658 4 13.012988470869585 17.054451973565723 38.034489299536816 31.89807025602787 5 13.738516823676575 37.57638280225334 34.21555547581035 14.46954489825973 6 30.814709340269854 37.03062708422802 17.974698721668155 14.179964853833967 7 27.904048430684846 32.147973324874236 22.123451095194575 17.824527149246343 8 26.466730219012803 34.700454194816906 20.054130242269892 18.7786853439004 9 26.646476698648435 16.111151460755334 20.487368561625587 36.75500327897065 10-11 25.179246621116537 26.11489524194088 27.695908368164655 21.00994976877793 12-13 25.8002952806737 23.673220236469454 27.975314833834076 22.551169649022768 14-15 23.36161573266741 25.114671578289073 25.497951056315966 26.025761632727555 16-17 22.64343077437806 28.014424591254265 26.317009841076278 23.025134793291397 18-19 22.385626221838546 27.181042038540216 28.368733796446904 22.064597943174334 20-21 23.343738434819944 26.289309100302933 28.194161972977188 22.172790491899935 22-23 23.361771924736665 26.081828838479094 27.9116927616268 22.64470647515745 24-25 23.022249217128778 26.31207065604438 28.13562179056874 22.530058336258097 26-27 22.842363302467813 26.451433288375693 27.981888523364024 22.724314885792467 28-29 22.61317502246439 26.44312898517614 28.266581918628358 22.677114073731115 30-31 23.096200104549013 26.26879616680604 28.114025508552988 22.520978220091962 32-33 23.033227532745883 26.54752195372395 27.81023347924333 22.609017034286836 34-35 23.12655954735065 26.516748828964925 27.97616404044458 22.38052758323985 36-37 23.14145363884733 26.313637751374568 27.784159028204286 22.760749581573812 38-39 23.060911782779197 26.38426877364691 27.995694557023924 22.55912488654997 40-41 22.93662792325975 26.07413679251361 28.312559161637076 22.67667612258957 42-43 22.88974708070724 26.14268394432911 27.92763359315141 23.03993538181224 44-45 22.754706078552566 26.500016613071757 27.808114974383923 22.93716233399175 46-47 22.916516683202122 26.33134730376944 27.536926816447078 23.21520919658136 48-49 23.19644790023391 26.400484439947952 27.4188828759618 22.984184783856342 50-51 22.986388217219154 26.436379342782608 27.294859264962344 23.282373175035893 52-53 22.83239872297135 26.647823223381177 27.25931209326024 23.260465960387233 54-55 22.854512604987875 26.575885060491046 27.239747985970254 23.32985434855083 56-57 23.061718863355914 26.4979919571795 27.025144130484847 23.41514504897974 58-59 22.693470910375513 26.433757556191782 27.48357215337495 23.38919938005775 60-61 22.933301343851102 26.769148018603524 27.11242796176788 23.185122675777492 62-63 22.91990991384821 26.564802814209855 27.124782269702674 23.390505002239266 64-65 22.580771592716527 26.397342110459466 27.242142986013583 23.77974331081042 66-67 22.79067216035623 26.349972665583586 27.05770637484392 23.801648799216263 68-69 22.48698543275979 26.473074113532064 27.20959519300885 23.8303452606993 70-71 22.609602670979122 26.18288188771573 27.268941740833608 23.938573700471537 72-73 22.73232268149151 26.429753824825937 26.99137302634466 23.8465504673379 74-75 22.65212406693336 26.615344041192706 26.941721349974717 23.790810541899216 76-77 22.51859275236102 26.49598937564615 26.991263609591204 23.994154262401622 78-79 22.452920144804406 26.360485827855527 27.033646982499643 24.152947044840424 80-81 22.386986089489803 26.75317887812269 26.877278889484863 23.982556142902645 82-83 22.723243668088784 26.33662380427999 27.1685477979156 23.771584729715624 84-85 22.648727765072376 26.45370052419726 26.790105521112746 24.10746618961762 86-87 22.2511186346517 26.640205280681943 27.104514149206597 24.004161935459752 88-89 22.37689168091267 26.23635054616989 27.061626352669087 24.325131420248354 90-91 22.4820998421265 26.178968242430127 27.122753114414994 24.216178801028377 92-93 22.498156248918377 26.294318584212167 26.968772048467354 24.2387531184021 94-95 22.05043186956622 26.07166578048275 27.20645685707747 24.67144549287356 96-97 22.16976134156545 26.022045638552438 27.20648330726322 24.6017097126189 98-99 21.972141773989108 26.18694225731219 27.084053879521857 24.756862089176845 100 21.95744657280464 25.99921472733505 26.995846320903826 25.04749237895648 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1505.0 1 1079.5 2 1461.5 3 1996.0 4 1325.0 5 1004.0 6 1466.0 7 2677.0 8 4255.0 9 5242.0 10 4944.5 11 4247.0 12 4381.0 13 5005.0 14 5852.5 15 6748.0 16 7430.5 17 8276.5 18 9120.0 19 9752.0 20 10535.5 21 11542.5 22 12910.5 23 14727.5 24 16850.0 25 19798.5 26 24532.5 27 30273.5 28 36601.0 29 47043.0 30 63424.0 31 81184.0 32 105470.5 33 135743.5 34 165704.5 35 195529.5 36 232624.5 37 272931.0 38 291116.0 39 294252.0 40 289710.5 41 282815.5 42 287633.5 43 302013.5 44 320022.5 45 344797.0 46 364341.0 47 374461.5 48 388517.0 49 394476.0 50 387497.5 51 378701.0 52 369458.5 53 360101.5 54 351032.5 55 328577.0 56 297276.5 57 260186.5 58 226075.0 59 200519.5 60 167757.0 61 134443.0 62 107934.0 63 84340.5 64 62535.0 65 44943.5 66 32557.5 67 24419.5 68 18465.5 69 13616.0 70 10492.0 71 8155.0 72 6372.0 73 5053.0 74 3849.5 75 2820.0 76 1982.5 77 1234.5 78 759.5 79 513.0 80 323.0 81 181.0 82 113.0 83 70.0 84 41.0 85 24.5 86 15.5 87 12.5 88 11.5 89 5.5 90 3.0 91 1.5 92 0.5 93 1.0 94 2.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03040120863705259 2 0.001959307316713722 3 5.32420466498294E-5 4 0.020242626136265138 5 5.643656944881916E-4 6 7.453886530976116E-5 7 3.1945227989897645E-5 8 0.004152879638686694 9 0.0099243174955282 10-11 0.0010861377516565196 12-13 9.583568396969293E-5 14-15 1.6505034461447116E-4 16-17 2.9815546123904466E-4 18-19 1.064840932996588E-5 20-21 7.98630699747441E-5 22-23 2.1829239126430053E-4 24-25 2.129681865993176E-5 26-27 1.2778091195959058E-4 28-29 0.001059516728331605 30-31 0.0028005316537810265 32-33 0.009844454425553456 34-35 0.01843239655017094 36-37 0.021536407869855993 38-39 0.02160029832583579 40-41 0.005185775343693384 42-43 0.00563300853555195 44-45 0.009349303391710043 46-47 0.012245670729460763 48-49 1.2004058321763835 50-51 0.018586798485455445 52-53 0.018932871788679338 54-55 0.00939722123369489 56-57 0.007576343238270724 58-59 0.008992581679156186 60-61 0.005191099548358367 62-63 0.007768014606210109 64-65 0.019380104980537903 66-67 0.02903288803815197 68-69 0.029261828838746236 70-71 0.02616314172372617 72-73 0.022894080059426645 74-75 0.009248143503075368 76-77 0.01412511497619974 78-79 0.008007603816134343 80-81 0.002779234835121095 82-83 0.014204978046174484 84-85 0.023873733717783504 86-87 0.023138993474015858 88-89 0.032594780959025564 90-91 0.020349110229564798 92-93 0.013299863253127384 94-95 0.006633959012568743 96-97 0.003971856680077273 98-99 0.015924696152963973 100 0.03462862714104904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9391074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.77332545667605 #Duplication Level Percentage of deduplicated Percentage of total 1 72.24887635383844 37.40564589346423 2 15.272609923865401 15.814276083222875 3 5.364810085915906 8.332621757741471 4 2.5398955945542747 5.259953649713446 5 1.3139957399547024 3.4014964546680333 6 0.7939425751989683 2.4663028403812604 7 0.5189905312425195 1.880890597806651 8 0.36465136508253726 1.5103371042111517 9 0.2502193801133154 1.165922046195697 >10 1.2036104997729655 10.902267144425213 >50 0.07224281468808234 2.5984624893526025 >100 0.04947740418793982 5.12416399487013 >500 0.00470991851571667 1.6064707705130052 >1k 0.001838776146525488 1.5232764022116296 >5k 3.2259230629352486E-5 0.08352508399423547 >10k+ 9.677769188805747E-5 0.9243876872283445 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24505 0.2609392706308139 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19813 0.21097693405461398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11029 0.1174413065001937 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.064840932996588E-5 2 0.0 0.0 0.0 0.0 1.064840932996588E-5 3 0.0 0.0 0.0 0.0 1.064840932996588E-5 4 0.0 0.0 0.0 0.0 2.129681865993176E-5 5 0.0 0.0 0.0 0.0 3.194522798989764E-5 6 0.0 0.0 0.0 0.0 4.259363731986352E-5 7 0.0 0.0 0.0 1.064840932996588E-5 4.259363731986352E-5 8 0.0 0.0 0.0 1.064840932996588E-5 5.32420466498294E-5 9 0.0 0.0 0.0 1.064840932996588E-5 6.389045597979528E-5 10-11 5.32420466498294E-6 0.0 0.0 1.597261399494882E-5 6.389045597979528E-5 12-13 1.064840932996588E-5 0.0 0.0 2.129681865993176E-5 8.518727463972705E-5 14-15 1.064840932996588E-5 0.0 0.0 2.129681865993176E-5 1.490777306195223E-4 16-17 1.597261399494882E-5 0.0 0.0 3.7269432654880576E-5 1.8102295860941995E-4 18-19 2.129681865993176E-5 0.0 0.0 6.921466064477823E-5 1.9167136793938586E-4 20-21 2.129681865993176E-5 0.0 0.0 1.8102295860941995E-4 1.9167136793938586E-4 22-23 2.129681865993176E-5 0.0 0.0 5.750141038181575E-4 2.236165959292835E-4 24-25 2.129681865993176E-5 0.0 0.0 0.0016505034461447114 2.66210233249147E-4 26-27 2.129681865993176E-5 0.0 0.0 0.0035831897395335183 2.66210233249147E-4 28-29 2.129681865993176E-5 0.0 0.0 0.008055521658119189 2.7153443791412996E-4 30-31 2.129681865993176E-5 0.0 0.0 0.020386379662219678 2.768586425791129E-4 32-33 2.129681865993176E-5 0.0 0.0 0.04954172440766626 2.9815546123904466E-4 34-35 2.129681865993176E-5 0.0 0.0 0.09235365411879408 3.301006892289423E-4 36-37 2.129681865993176E-5 0.0 0.0 0.15992313552209259 3.354248938939252E-4 38-39 2.129681865993176E-5 0.0 0.0 0.25609956859034444 3.620459172188399E-4 40-41 2.129681865993176E-5 0.0 0.0 0.3606296787779545 3.726943265488058E-4 42-43 2.129681865993176E-5 0.0 0.0 0.4652928940821891 3.939911452087376E-4 44-45 2.129681865993176E-5 0.0 0.0 0.5938245188995424 4.099637592036864E-4 46-47 2.129681865993176E-5 0.0 0.0 0.7470072113157664 4.41908987193584E-4 48-49 2.129681865993176E-5 0.0 0.0 0.9135270364177729 4.791784198484646E-4 50-51 2.66210233249147E-5 0.0 0.0 1.090487626867811 4.791784198484646E-4 52-53 3.194522798989764E-5 0.0 0.0 1.2673630300432093 5.590414898232087E-4 54-55 3.194522798989764E-5 0.0 0.0 1.460232344032216 6.389045597979528E-4 56-57 3.194522798989764E-5 1.064840932996588E-5 0.0 1.6885448884760146 6.548771737929016E-4 58-59 3.194522798989764E-5 1.064840932996588E-5 0.0 1.9432282186254737 6.602013784578846E-4 60-61 3.194522798989764E-5 1.064840932996588E-5 0.0 2.20649949089955 6.602013784578846E-4 62-63 3.194522798989764E-5 1.064840932996588E-5 0.0 2.4780924950649945 7.027950157777481E-4 64-65 3.194522798989764E-5 1.064840932996588E-5 0.0 2.7500528693523236 7.027950157777481E-4 66-67 3.194522798989764E-5 1.064840932996588E-5 0.0 3.0548529380132665 7.13443425107714E-4 68-69 3.194522798989764E-5 1.064840932996588E-5 0.0 3.3877062410540053 7.240918344376798E-4 70-71 3.7269432654880576E-5 1.064840932996588E-5 0.0 3.7433151948328804 7.400644484326286E-4 72-73 5.32420466498294E-5 1.064840932996588E-5 0.0 4.100287145005992 9.423842257019804E-4 74-75 5.856625131481234E-5 1.064840932996588E-5 0.0 4.451311958568317 9.530326350319462E-4 76-77 6.389045597979528E-5 1.064840932996588E-5 0.0 4.83447367148848 9.583568396969292E-4 78-79 6.389045597979528E-5 1.064840932996588E-5 0.0 5.250869069927465 9.583568396969292E-4 80-81 6.389045597979528E-5 1.064840932996588E-5 0.0 5.691548165843438 9.956262723518098E-4 82-83 6.389045597979528E-5 1.064840932996588E-5 0.0 6.141065441503283 0.0010754893423265538 84-85 6.389045597979528E-5 1.064840932996588E-5 0.0 6.5829584560828724 0.0010808135469915368 86-87 6.389045597979528E-5 1.064840932996588E-5 0.0 7.040850705680734 0.0010967861609864856 88 6.389045597979528E-5 1.064840932996588E-5 0.0 7.400048173403809 0.0011074345703164516 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16325 0.0 45.680214 1 GTATCAA 28930 0.0 40.214767 1 CAACGCA 35335 0.0 32.182743 5 ATCAACG 35760 0.0 31.832937 3 TCAACGC 37135 0.0 30.729908 4 TATCAAC 37655 0.0 30.677252 2 AACGCAG 37975 0.0 30.254261 6 ACGCAGA 42900 0.0 26.584175 7 CGCAGAG 44420 0.0 25.642675 8 GTACATG 41500 0.0 24.765863 1 TACATGG 43465 0.0 23.575668 2 GCAGAGT 48440 0.0 23.32886 9 ACATGGG 43430 0.0 22.926565 3 CATGGGG 29230 0.0 21.141611 4 GAGTACT 23520 0.0 20.840025 12-13 GTGGTAT 9360 0.0 20.80797 1 TAGTACT 4785 0.0 20.494883 4 AGAGTAC 44535 0.0 19.631962 10-11 TACCTGG 5850 0.0 19.569738 2 CAGAGTA 47895 0.0 19.273214 10-11 >>END_MODULE