FastQCFastQC Report
Mon 6 Jun 2016
ERR1211284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9996940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274430.274514001284393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197840.19790055757061661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116180.11621556196196037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137800.047.1407931
GTATCAA256000.043.4370081
ATCAACG312900.034.4478263
CAACGCA314600.034.1866655
TATCAAC324700.033.7677042
TCAACGC324250.033.2703364
AACGCAG336200.032.4668926
ACGCAGA379500.028.453537
CGCAGAG391650.027.4503028
TACCTGG75950.026.8164732
GTACATG354550.025.41971
GCAGAGT438300.024.134179
TACATGG366350.023.9583552
GTACCTG92100.022.9829241
ACATGGG368750.022.9740583
GTGGTAT72600.021.8346231
CATGGGG260800.021.2408314
GTACTAG33100.020.748051
GAGTACT241100.020.3577512-13
AGAGTAC404000.020.152510-11
Produced by FastQC (version 0.11.5)