##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211284_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9996940 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.349952285399333 31.0 31.0 34.0 28.0 34.0 2 31.360592541317644 31.0 31.0 34.0 28.0 34.0 3 31.414065404013627 31.0 31.0 34.0 28.0 34.0 4 35.01778174121281 37.0 35.0 37.0 32.0 37.0 5 35.06264717003403 37.0 35.0 37.0 32.0 37.0 6 35.12199563066298 37.0 35.0 37.0 32.0 37.0 7 35.05965285377326 37.0 35.0 37.0 32.0 37.0 8 35.05796793818909 37.0 35.0 37.0 32.0 37.0 9 36.61577842819903 39.0 37.0 39.0 32.0 39.0 10-11 36.54520773356647 39.0 37.0 39.0 32.0 39.0 12-13 36.465767424831995 39.0 37.0 39.0 32.0 39.0 14-15 37.59850939387452 40.0 37.0 41.0 32.0 41.0 16-17 37.596195535833964 40.0 37.0 41.0 32.0 41.0 18-19 37.59277538926912 40.0 37.0 41.0 32.0 41.0 20-21 37.55674401366818 40.0 37.0 41.0 32.0 41.0 22-23 37.438275062169026 40.0 37.0 41.0 31.0 41.0 24-25 37.326100186657115 40.0 37.0 41.0 31.0 41.0 26-27 37.1872175385668 40.0 36.5 41.0 31.0 41.0 28-29 37.05360950450838 40.0 36.0 41.0 30.0 41.0 30-31 36.87518545674976 39.0 36.0 41.0 30.0 41.0 32-33 36.75717549570169 39.0 36.0 41.0 30.0 41.0 34-35 36.6158998153435 39.0 35.0 41.0 30.0 41.0 36-37 36.5241819496766 39.0 35.0 41.0 29.5 41.0 38-39 36.37784887175476 39.0 35.0 41.0 29.0 41.0 40-41 36.22227526623146 39.0 35.0 40.0 28.5 41.0 42-43 36.079448461229134 39.0 35.0 40.0 28.0 41.0 44-45 35.95593176512013 39.0 35.0 40.0 27.0 41.0 46-47 35.807865406814486 39.0 35.0 40.0 27.0 41.0 48-49 35.58958981448323 38.5 34.0 40.0 26.0 41.0 50-51 34.9341290434873 37.5 33.5 39.5 25.0 40.5 52-53 35.182224210608446 38.0 34.0 40.0 26.0 40.5 54-55 35.555108063067294 38.0 34.0 40.0 26.0 41.0 56-57 35.46560412486221 38.0 34.0 40.0 26.0 41.0 58-59 35.253186325015456 38.0 34.0 40.0 26.0 41.0 60-61 34.99327279147419 38.0 34.0 40.0 26.0 41.0 62-63 34.72698095617259 37.0 33.5 40.0 26.0 41.0 64-65 34.45599713512335 37.0 33.0 40.0 25.0 41.0 66-67 34.23629065494041 36.0 33.0 39.5 25.0 41.0 68-69 33.90177434294894 36.0 33.0 39.0 24.0 41.0 70-71 33.55486493867123 35.5 32.0 39.0 24.0 40.5 72-73 33.18648791530208 35.0 32.0 38.0 23.0 40.0 74-75 32.81305884600688 35.0 32.0 37.0 23.0 39.5 76-77 32.44739140176894 35.0 31.0 37.0 22.0 39.0 78-79 32.07248423017443 35.0 31.0 36.0 21.5 39.0 80-81 31.724377309456692 35.0 31.0 36.0 20.0 37.5 82-83 31.401253583596578 34.5 30.5 35.5 20.0 37.0 84-85 31.092245127008866 34.0 30.0 35.0 20.0 37.0 86-87 30.828190376255137 34.0 30.0 35.0 19.0 36.0 88-89 30.562479118610295 34.0 29.5 35.0 18.5 36.0 90-91 30.29978128307262 34.0 29.0 35.0 18.0 36.0 92-93 30.061030575356057 34.0 29.0 35.0 17.5 35.0 94-95 29.838048742915333 34.0 29.0 35.0 17.0 35.0 96-97 29.634526515113624 34.0 29.0 35.0 15.5 35.0 98-99 29.394406138278313 34.0 27.5 35.0 14.0 35.0 100 27.716470239893408 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.35159716144565323 1101 2 -0.39647785088521914 1101 3 -0.41935235221593814 1101 4 -0.36214638922999143 1101 5 -0.3478319711026714 1101 6 -0.36939309320121794 1101 7 -0.4467725637449078 1101 8 -0.5304462705068218 1101 9 -0.4981220344553705 1101 10-11 -0.6005701569591722 1101 12-13 -0.5557931374330707 1101 14-15 -0.5954146534199438 1101 16-17 -0.5938949017348136 1101 18-19 -0.6427890245461754 1101 20-21 -0.6669484315942427 1101 22-23 -0.7108824235189886 1101 24-25 -0.7728719265428339 1101 26-27 -0.7541139048813363 1101 28-29 -0.8236523433517036 1101 30-31 -0.8289454803005682 1101 32-33 -0.756494867755066 1101 34-35 -0.8373705509306504 1101 36-37 -0.8895351602638115 1101 38-39 -0.9272844442348998 1101 40-41 -0.9284339360316025 1101 42-43 -0.9821930750573102 1101 44-45 -1.0177373285539844 1101 46-47 -0.9418892764054547 1101 48-49 -0.9408870725490601 1101 50-51 -0.8597021808701513 1101 52-53 -0.7038693586801443 1101 54-55 -0.6551720763935833 1101 56-57 -0.7311746612146237 1101 58-59 -0.8341941281183338 1101 60-61 -0.8608468009118582 1101 62-63 -0.7723137344631681 1101 64-65 -0.7091287394608443 1101 66-67 -0.8824642629560984 1101 68-69 -0.8307861694205627 1101 70-71 -0.8502985541890595 1101 72-73 -0.8509390845466314 1101 74-75 -0.9023118986369347 1101 76-77 -0.8565304824888678 1101 78-79 -0.939884309520572 1101 80-81 -0.9550980696294644 1101 82-83 -1.0559645264006257 1101 84-85 -1.0121313693933764 1101 86-87 -1.0599006975058778 1101 88-89 -1.0536778498662684 1101 90-91 -1.0993698425690042 1101 92-93 -1.1525594907634904 1101 94-95 -1.146907891401817 1101 96-97 -1.2697141439348663 1101 98-99 -1.1561209594906217 1101 100 -1.1721508892146844 1102 1 -0.3786953336669363 1102 2 -0.42412969130316114 1102 3 -0.39051307390371903 1102 4 -0.2089131475400734 1102 5 -0.25686450415536655 1102 6 -0.2654476931349734 1102 7 -0.3183275274464492 1102 8 -0.34411149742647495 1102 9 -0.41076992137395507 1102 10-11 -0.34130698506962887 1102 12-13 -0.35393112137380456 1102 14-15 -0.5153881027650442 1102 16-17 -0.5316142719205246 1102 18-19 -0.5342185396188199 1102 20-21 -0.5588688007691971 1102 22-23 -0.5910506863364731 1102 24-25 -0.5535239820504998 1102 26-27 -0.5922094528551938 1102 28-29 -0.6409061366162305 1102 30-31 -0.627922218646404 1102 32-33 -0.7218694339621408 1102 34-35 -0.6817864830045792 1102 36-37 -0.6103292042778676 1102 38-39 -0.7825555241419409 1102 40-41 -0.7776947736437023 1102 42-43 -0.7387877208386655 1102 44-45 -0.748339132786171 1102 46-47 -0.7804335647036211 1102 48-49 -0.8257001463365441 1102 50-51 -0.8396209128672112 1102 52-53 -0.7012105810137683 1102 54-55 -0.6914452961186157 1102 56-57 -0.6619009984480542 1102 58-59 -0.6689016436639434 1102 60-61 -0.7539224973396514 1102 62-63 -0.7457520321751048 1102 64-65 -0.8335235879028247 1102 66-67 -0.6601097551072286 1102 68-69 -0.6376384007440166 1102 70-71 -0.7230202045295826 1102 72-73 -0.8355902425099728 1102 74-75 -0.7698594886705763 1102 76-77 -0.7719000948829269 1102 78-79 -0.7360468236227007 1102 80-81 -0.8353835875455218 1102 82-83 -0.8709397184945971 1102 84-85 -0.8359889392462776 1102 86-87 -0.8604884629630831 1102 88-89 -0.9184562456891712 1102 90-91 -0.9804988904168503 1102 92-93 -0.9025534544977596 1102 94-95 -0.933200028579904 1102 96-97 -0.9591724616734503 1102 98-99 -0.9843950956073897 1102 100 -1.0798216458122027 1103 1 -0.47086358551604945 1103 2 -0.4705201118619584 1103 3 -0.4941726485009674 1103 4 -0.32304876253108716 1103 5 -0.32591349333754494 1103 6 -0.40445013737070923 1103 7 -0.4892110719955127 1103 8 -0.5409570345272314 1103 9 -0.6420113164019625 1103 10-11 -0.6316894499104961 1103 12-13 -0.7294963777630841 1103 14-15 -0.7613009829489314 1103 16-17 -0.7758307568904854 1103 18-19 -0.7150747202620806 1103 20-21 -0.84549773350858 1103 22-23 -0.8554080987096242 1103 24-25 -0.8129547404667719 1103 26-27 -0.7960174446434962 1103 28-29 -0.7685157433758079 1103 30-31 -0.8353426481873782 1103 32-33 -0.9063652189645026 1103 34-35 -0.9831682615977471 1103 36-37 -0.9906330857889643 1103 38-39 -1.000415658108217 1103 40-41 -0.933535265192333 1103 42-43 -0.9798668674834587 1103 44-45 -1.0206676549657843 1103 46-47 -1.1142934639342243 1103 48-49 -1.075229132017924 1103 50-51 -1.0524176112898047 1103 52-53 -0.9906400013355565 1103 54-55 -1.0039279976428617 1103 56-57 -0.9891429454084175 1103 58-59 -1.007175003467438 1103 60-61 -0.9378374380372705 1103 62-63 -1.0196682578481528 1103 64-65 -1.0532520837252548 1103 66-67 -1.0076025859308615 1103 68-69 -0.9215755376214716 1103 70-71 -0.9489708776619352 1103 72-73 -1.1267626763470133 1103 74-75 -1.0086084411500096 1103 76-77 -0.9578629145743136 1103 78-79 -0.9508082317635242 1103 80-81 -0.9760781774701499 1103 82-83 -1.061784331829557 1103 84-85 -1.0362117104779074 1103 86-87 -1.1550826244698236 1103 88-89 -1.1838398677667712 1103 90-91 -1.052290935356588 1103 92-93 -1.0641078371619237 1103 94-95 -1.2783041077950976 1103 96-97 -1.3330672247156379 1103 98-99 -1.3862816064766896 1103 100 -1.3629571780892427 1104 1 -0.33381222028949153 1104 2 -0.35245736120037563 1104 3 -0.41076035757407325 1104 4 -0.3134834063862755 1104 5 -0.18353961581629363 1104 6 -0.3021940104595231 1104 7 -0.273466241359273 1104 8 -0.30441881726883224 1104 9 -0.41690837091206845 1104 10-11 -0.3125081312124749 1104 12-13 -0.4092143143973743 1104 14-15 -0.5496006066382719 1104 16-17 -0.5280146415260063 1104 18-19 -0.5437602851766385 1104 20-21 -0.5632392150761376 1104 22-23 -0.47787928241035615 1104 24-25 -0.5266335823626562 1104 26-27 -0.5855482854461798 1104 28-29 -0.5499827977428495 1104 30-31 -0.6150728166906703 1104 32-33 -0.6807767790925112 1104 34-35 -0.6422933692921831 1104 36-37 -0.6513190508799696 1104 38-39 -0.6466153704684245 1104 40-41 -0.722867419970278 1104 42-43 -0.728358187946391 1104 44-45 -0.7551643138655422 1104 46-47 -0.9059235694485182 1104 48-49 -0.7775482541045307 1104 50-51 -0.7480930408636297 1104 52-53 -0.6627969664701183 1104 54-55 -0.6795691861372575 1104 56-57 -0.6884602746818089 1104 58-59 -0.690322045308335 1104 60-61 -0.5987755580238385 1104 62-63 -0.7017184712949529 1104 64-65 -0.6745291375469904 1104 66-67 -0.6812986651134096 1104 68-69 -0.6027132531471651 1104 70-71 -0.7095935350168787 1104 72-73 -0.7761074714229963 1104 74-75 -0.7023916743449305 1104 76-77 -0.6758021432492853 1104 78-79 -0.6323098827631668 1104 80-81 -0.7215568396226715 1104 82-83 -0.7443874193306961 1104 84-85 -0.8593067083785613 1104 86-87 -0.8252703464110382 1104 88-89 -0.7294091144390578 1104 90-91 -0.7360447772271499 1104 92-93 -0.8090732698087244 1104 94-95 -0.7867339422686683 1104 96-97 -0.9140297506226496 1104 98-99 -0.9184927342925704 1104 100 -0.9353805536022826 1105 1 -0.3981366535019575 1105 2 -0.45173857571892384 1105 3 -0.395230699661024 1105 4 -0.30666799232921704 1105 5 -0.2622919507245456 1105 6 -0.3323385916410828 1105 7 -0.2765730428096589 1105 8 -0.3066983286778324 1105 9 -0.4405950994766954 1105 10-11 -0.46927238330932397 1105 12-13 -0.4544738783647091 1105 14-15 -0.6209337705763076 1105 16-17 -0.6579335149098853 1105 18-19 -0.612618217455136 1105 20-21 -0.6528621502847187 1105 22-23 -0.6795597296139633 1105 24-25 -0.5757210223875617 1105 26-27 -0.6498493695761312 1105 28-29 -0.7748908893445048 1105 30-31 -0.7232999345898037 1105 32-33 -0.735528088472428 1105 34-35 -0.841616360355431 1105 36-37 -0.767983428420898 1105 38-39 -0.7476883981576918 1105 40-41 -0.8414400127256627 1105 42-43 -0.8937308118338692 1105 44-45 -0.9783191640651552 1105 46-47 -0.9254304688734933 1105 48-49 -0.8470401597559203 1105 50-51 -0.8486719607625375 1105 52-53 -0.7319564910644871 1105 54-55 -0.6251312580707449 1105 56-57 -0.6885915560428302 1105 58-59 -0.6323268158463691 1105 60-61 -0.6762383139480974 1105 62-63 -0.6701355179564956 1105 64-65 -0.6315774701477963 1105 66-67 -0.6712743545980828 1105 68-69 -0.6654477740789275 1105 70-71 -0.6547695458948013 1105 72-73 -0.6831547739172308 1105 74-75 -0.6501948003750329 1105 76-77 -0.703321783948109 1105 78-79 -0.6105597545721224 1105 80-81 -0.6763832323267671 1105 82-83 -0.7014922933372141 1105 84-85 -0.7171212113635796 1105 86-87 -0.706505696006321 1105 88-89 -0.7151131808420175 1105 90-91 -0.7735428314702695 1105 92-93 -0.7548526942309515 1105 94-95 -0.8005741109314499 1105 96-97 -0.9163710684265531 1105 98-99 -0.9390300836680936 1105 100 -0.8782785410029348 1106 1 -0.4041451829030933 1106 2 -0.4643986208768176 1106 3 -0.45054694042828913 1106 4 -0.3082213766981923 1106 5 -0.2794040617513218 1106 6 -0.27322219974515605 1106 7 -0.34752452565859926 1106 8 -0.3396070228082593 1106 9 -0.4539256770183471 1106 10-11 -0.4340730829808095 1106 12-13 -0.470226550697987 1106 14-15 -0.5797833393032263 1106 16-17 -0.6774033852378807 1106 18-19 -0.7116990753314241 1106 20-21 -0.6626306828054425 1106 22-23 -0.6197256549037107 1106 24-25 -0.6089857640897591 1106 26-27 -0.6224609137515387 1106 28-29 -0.6842794305296778 1106 30-31 -0.7110161417834959 1106 32-33 -0.7400450664317972 1106 34-35 -0.6614031051163423 1106 36-37 -0.713543084772013 1106 38-39 -0.7238786968674376 1106 40-41 -0.760091919381594 1106 42-43 -0.7285847168311079 1106 44-45 -0.810857532341636 1106 46-47 -0.7483949457914179 1106 48-49 -0.8196282594913242 1106 50-51 -0.7712566960068088 1106 52-53 -0.628923717354219 1106 54-55 -0.749740917655572 1106 56-57 -0.7042307392157099 1106 58-59 -0.688808026530566 1106 60-61 -0.6610635494387012 1106 62-63 -0.6286462479488222 1106 64-65 -0.6298026059638673 1106 66-67 -0.6801604987913734 1106 68-69 -0.5911970628992123 1106 70-71 -0.6261444731362005 1106 72-73 -0.6522843669826202 1106 74-75 -0.6377373498936905 1106 76-77 -0.710449238481111 1106 78-79 -0.6753369681932924 1106 80-81 -0.7095961097442043 1106 82-83 -0.5915125528048684 1106 84-85 -0.6225600658387549 1106 86-87 -0.6498091103347257 1106 88-89 -0.7101510379060691 1106 90-91 -0.6135126354595357 1106 92-93 -0.664546250909428 1106 94-95 -0.7167694100121267 1106 96-97 -0.6813308562187075 1106 98-99 -0.6725411168212929 1106 100 -0.7676421005442045 1107 1 -0.5450986250337202 1107 2 -0.5542686820555112 1107 3 -0.475410205548112 1107 4 -0.3894955699571625 1107 5 -0.31878759550890834 1107 6 -0.34784559129445114 1107 7 -0.3791741520199068 1107 8 -0.4158653866989255 1107 9 -0.5209873344458913 1107 10-11 -0.5217483639316853 1107 12-13 -0.5980184522356495 1107 14-15 -0.7329937414483609 1107 16-17 -0.7134759321384294 1107 18-19 -0.6930849145915445 1107 20-21 -0.7032157669586496 1107 22-23 -0.6668975271830888 1107 24-25 -0.7367520106692993 1107 26-27 -0.7406633029116989 1107 28-29 -0.7513522137437789 1107 30-31 -0.6930854164589135 1107 32-33 -0.7041959739285844 1107 34-35 -0.757272948248044 1107 36-37 -0.7773014374332305 1107 38-39 -0.8390025313635547 1107 40-41 -0.8881620001352317 1107 42-43 -0.873766355201532 1107 44-45 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2311 24-25 0.8542476784795099 2311 26-27 0.8611955174862445 2311 28-29 0.8849939255654391 2311 30-31 0.8410313127832865 2311 32-33 0.9091172982863966 2311 34-35 0.9251100928116429 2311 36-37 0.9288438494009839 2311 38-39 0.9481828307686655 2311 40-41 0.9756794551988079 2311 42-43 0.9886329975342676 2311 44-45 1.0060107893120787 2311 46-47 1.0576572484788969 2311 48-49 1.0276339089562327 2311 50-51 0.9632422385556296 2311 52-53 0.8651840699654159 2311 54-55 0.8740753181524212 2311 56-57 0.7810026059926898 2311 58-59 0.7673059033740373 2311 60-61 0.7963887603369884 2311 62-63 0.788809447329399 2311 64-65 0.7737240698045653 2311 66-67 0.7902685749307139 2311 68-69 0.8279381071614367 2311 70-71 0.8036749446016245 2311 72-73 0.7213185600387746 2311 74-75 0.7408996866188531 2311 76-77 0.79178895485542 2311 78-79 0.8088210927324724 2311 80-81 0.7926180752901075 2311 82-83 0.786528369733503 2311 84-85 0.862778606114901 2311 86-87 0.8648282094093922 2311 88-89 0.9395693815422064 2311 90-91 0.9396895409981667 2311 92-93 0.8788144024704287 2311 94-95 0.9493084078655407 2311 96-97 0.9625299885637482 2311 98-99 0.9849231417868438 2311 100 1.08207566360797 2312 1 0.5272605876365084 2312 2 0.5355095012571702 2312 3 0.5587769212114821 2312 4 0.46043342248335506 2312 5 0.4565966471451972 2312 6 0.4930261395950737 2312 7 0.5202166330562719 2312 8 0.5261489643845749 2312 9 0.7137560005756924 2312 10-11 0.6902878542082576 2312 12-13 0.7053226590435031 2312 14-15 0.8955505017532843 2312 16-17 0.8388791361520234 2312 18-19 0.8043223997045743 2312 20-21 0.8321715773029723 2312 22-23 0.84806375057682 2312 24-25 0.9053320514639083 2312 26-27 0.9368313349352775 2312 28-29 0.932058848968019 2312 30-31 0.9217486809248854 2312 32-33 0.9301793637621358 2312 34-35 0.9427731140180313 2312 36-37 0.9634472140574815 2312 38-39 1.0101903996422053 2312 40-41 1.05506603434592 2312 42-43 1.0443680940872966 2312 44-45 1.0336924119873387 2312 46-47 1.0825563123474993 2312 48-49 1.0251186126334062 2312 50-51 1.047757406592467 2312 52-53 0.9162370841517102 2312 54-55 0.912862766080778 2312 56-57 0.8873385086093748 2312 58-59 0.8921957021432121 2312 60-61 0.9709763743244437 2312 62-63 0.9893890505693292 2312 64-65 0.9447617253067833 2312 66-67 0.8841635871000619 2312 68-69 0.9233490321390718 2312 70-71 0.8868987003165856 2312 72-73 0.933244346592268 2312 74-75 0.9122017538050713 2312 76-77 0.8992560904786586 2312 78-79 0.9062279339393626 2312 80-81 0.8818370630423544 2312 82-83 0.911237142713631 2312 84-85 0.9527022759495196 2312 86-87 0.9834767164811566 2312 88-89 1.0123661587130002 2312 90-91 1.0619469824765915 2312 92-93 1.0611920051615833 2312 94-95 1.07269387520072 2312 96-97 0.9965430594809526 2312 98-99 1.1063417354160237 2312 100 1.2186802897433928 2313 1 0.48723063656670007 2313 2 0.5024187353818022 2313 3 0.5371862323635135 2313 4 0.41716589780204316 2313 5 0.4431872356927613 2313 6 0.4154185484512425 2313 7 0.47146478316093976 2313 8 0.46979366673775047 2313 9 0.5754145891095916 2313 10-11 0.6092869228516733 2313 12-13 0.6059809712960771 2313 14-15 0.8139952206782226 2313 16-17 0.8060866417041765 2313 18-19 0.764053371751217 2313 20-21 0.7234509553700548 2313 22-23 0.7286174602688718 2313 24-25 0.774026013504546 2313 26-27 0.8162508411981548 2313 28-29 0.8139643116319561 2313 30-31 0.9120873518197854 2313 32-33 0.9088800803163437 2313 34-35 0.9742697355347687 2313 36-37 0.959268201148582 2313 38-39 0.982168912451634 2313 40-41 0.9753327781889709 2313 42-43 0.8437886647533972 2313 44-45 0.9657362290122578 2313 46-47 0.9921059204979841 2313 48-49 1.042271569213142 2313 50-51 0.9876902039679933 2313 52-53 0.8988040238356163 2313 54-55 0.8518846017870345 2313 56-57 0.8796500161987737 2313 58-59 0.8462636141089774 2313 60-61 0.8795728013336443 2313 62-63 0.9005096692757917 2313 64-65 0.911421660635078 2313 66-67 0.8743499338119065 2313 68-69 0.8489922726195189 2313 70-71 0.8877131576482853 2313 72-73 0.8604359830104258 2313 74-75 0.9026233512373025 2313 76-77 0.8984038643642549 2313 78-79 0.8880235386929343 2313 80-81 0.9144842611073258 2313 82-83 0.9064301085748738 2313 84-85 0.8766340664986174 2313 86-87 0.9777356658767964 2313 88-89 0.9445660546022339 2313 90-91 1.007024840645684 2313 92-93 1.028253766628385 2313 94-95 1.046633607013547 2313 96-97 1.0813015313699275 2313 98-99 1.0921321630134173 2313 100 1.1569114941664331 2314 1 0.2953487905816736 2314 2 0.17642837430011582 2314 3 0.29307739807005717 2314 4 0.22709110043345504 2314 5 0.21035802363881118 2314 6 0.20564438172979038 2314 7 0.26120704876262835 2314 8 0.2928584647327952 2314 9 0.40057343296790293 2314 10-11 0.41209973744077644 2314 12-13 0.45340904275838767 2314 14-15 0.5397001797605654 2314 16-17 0.5075092644020884 2314 18-19 0.5012442820576126 2314 20-21 0.5287877850989418 2314 22-23 0.5661051477349375 2314 24-25 0.5934468567723954 2314 26-27 0.6391783344900546 2314 28-29 0.6486367371371031 2314 30-31 0.6961674978778802 2314 32-33 0.6928396069582519 2314 34-35 0.7004434108787478 2314 36-37 0.6236330518928597 2314 38-39 0.6808638792307917 2314 40-41 0.7442785404081604 2314 42-43 0.5585952075600247 2314 44-45 0.6714452617939699 2314 46-47 0.5614729075923677 2314 48-49 0.5384691024184818 2314 50-51 0.6838343472010138 2314 52-53 0.6688765124406828 2314 54-55 0.59910618297247 2314 56-57 0.5579859427053222 2314 58-59 0.6027533511366272 2314 60-61 0.4160607326236345 2314 62-63 0.5594290882586677 2314 64-65 0.5944831616380384 2314 66-67 0.5981221835555388 2314 68-69 0.6423703620471457 2314 70-71 0.5955991214045326 2314 72-73 0.6707418834242915 2314 74-75 0.7336418020051241 2314 76-77 0.7125313877994159 2314 78-79 0.6669322658352428 2314 80-81 0.6220688991127545 2314 82-83 0.6574306734221054 2314 84-85 0.7457686136625838 2314 86-87 0.7655846761597793 2314 88-89 0.6038202767016045 2314 90-91 0.563855174793467 2314 92-93 0.7449111132003772 2314 94-95 0.8101734614583336 2314 96-97 0.8007168037920174 2314 98-99 0.7871674361740659 2314 100 0.8511457851731272 2315 1 0.2748544490557059 2315 2 0.2839160417468385 2315 3 0.2724102578599634 2315 4 0.2300264614124501 2315 5 0.21749844338473423 2315 6 0.21920277170368507 2315 7 0.22782198689496624 2315 8 0.1897346755338063 2315 9 0.11949488601963765 2315 10-11 0.2545994858508607 2315 12-13 0.2258719957601869 2315 14-15 0.39029829159422746 2315 16-17 0.28752223495762763 2315 18-19 0.388743157464674 2315 20-21 0.2879719500695046 2315 22-23 0.29462077829332856 2315 24-25 0.4359538200245012 2315 26-27 0.4902292152005998 2315 28-29 0.3731391743879655 2315 30-31 0.4269992035576351 2315 32-33 0.47680072641056626 2315 34-35 0.42009875065340907 2315 36-37 0.5281330053503339 2315 38-39 0.6307754856177255 2315 40-41 0.6543138293517146 2315 42-43 0.6780722799576111 2315 44-45 0.6847289943874131 2315 46-47 0.6985916666521277 2315 48-49 0.6113374516015639 2315 50-51 0.5135200106206099 2315 52-53 0.24405353599043167 2315 54-55 0.4144886830778347 2315 56-57 0.3816934445150011 2315 58-59 0.38335161413159824 2315 60-61 0.5116712577753262 2315 62-63 0.57528597745533 2315 64-65 0.5564457601258184 2315 66-67 0.5599997019662197 2315 68-69 0.5338590307909499 2315 70-71 0.4891109348181075 2315 72-73 0.517155869543565 2315 74-75 0.2711522626322491 2315 76-77 0.5003447502331255 2315 78-79 0.5265617903474151 2315 80-81 0.5227319044818728 2315 82-83 0.48463378049203243 2315 84-85 0.5314354819396314 2315 86-87 0.44663508021882237 2315 88-89 0.4333251471919013 2315 90-91 0.6239804755054337 2315 92-93 0.7474280598203151 2315 94-95 0.6959159840396794 2315 96-97 0.6968042586032617 2315 98-99 0.7538699177264867 2315 100 0.7594193155223223 2316 1 -0.00364888721388823 2316 2 -4.632331839147241E-4 2316 3 0.04113784555775979 2316 4 -0.067771439092418 2316 5 -0.03330822466928396 2316 6 -0.22316817768450647 2316 7 -0.18164370599075141 2316 8 -0.10475215433368845 2316 9 -0.04363339692823587 2316 10-11 -0.07965364961076915 2316 12-13 -0.07792355798629558 2316 14-15 -0.19189570324108018 2316 16-17 -0.04599386114757209 2316 18-19 0.016353510209079047 2316 20-21 0.03119662566005843 2316 22-23 -0.05826023415978199 2316 24-25 -0.0687553104619596 2316 26-27 -0.14317900766525327 2316 28-29 -0.08723649021946756 2316 30-31 -0.6858674081134168 2316 32-33 -0.5174540319121448 2316 34-35 -0.5795568435030418 2316 36-37 -0.41617078845516176 2316 38-39 -0.25264527644214496 2316 40-41 -0.07651289900135794 2316 42-43 -0.011275879686586165 2316 44-45 -0.10313147428425395 2316 46-47 -0.20926973281355998 2316 48-49 -0.159608488315925 2316 50-51 0.023342419462473174 2316 52-53 -0.3910467546536154 2316 54-55 -0.28368755143596047 2316 56-57 -0.3257118176542235 2316 58-59 -0.7177719217667899 2316 60-61 -0.1927344845003489 2316 62-63 -0.22984530187764562 2316 64-65 -0.5533770224804826 2316 66-67 -0.8449800339728242 2316 68-69 -0.8601272079258848 2316 70-71 -0.6259311667315188 2316 72-73 -0.8523646533794036 2316 74-75 -0.6425009784962157 2316 76-77 -0.2934344591562166 2316 78-79 -0.201968999522947 2316 80-81 -0.30037726342927584 2316 82-83 -0.11673879103381779 2316 84-85 -0.11794562443686374 2316 86-87 0.05245581025551971 2316 88-89 0.09912753383457229 2316 90-91 -0.5213881490679597 2316 92-93 -0.5849675869573439 2316 94-95 -0.22749395573119457 2316 96-97 0.027829033880404808 2316 98-99 0.029779991931988548 2316 100 -0.17109112371841206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 311.0 1 738.0 2 413.0 3 421.0 4 426.0 5 317.0 6 395.0 7 922.0 8 2388.0 9 4651.0 10 7619.0 11 12577.0 12 19151.0 13 24707.0 14 28990.0 15 32968.0 16 38454.0 17 45899.0 18 55145.0 19 64403.0 20 75826.0 21 90129.0 22 100170.0 23 103782.0 24 105929.0 25 112046.0 26 123003.0 27 141621.0 28 165087.0 29 196032.0 30 237499.0 31 291762.0 32 367047.0 33 476822.0 34 648214.0 35 894784.0 36 1320096.0 37 1934977.0 38 1848361.0 39 422573.0 40 285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2312922120764 18.928639966445456 13.280899024801105 23.559168796677042 2 15.75579444914554 20.395359433598543 40.42497133443823 23.423874782817684 3 19.019552816917262 26.090552148098567 29.396327349982265 25.493567685001906 4 12.895952953370571 16.81263687833848 38.528132031507155 31.763278136783796 5 13.70711411773004 37.201526565572635 34.46691515795171 14.62444415874561 6 30.34270451781755 36.75901080114235 18.827347148609103 14.070937532431 7 27.435253196758946 32.090954362095324 22.530950807361023 17.942841633784706 8 25.742598432556267 34.80316290683378 20.794016983342285 18.660221677267664 9 26.462408922147347 16.54170729247218 20.75892606983956 36.23695771554091 10-11 24.790884139556404 26.09305127934177 28.432317008737083 20.683747572364744 12-13 25.551385283367118 23.761770637030967 28.363824852570747 22.323019227031168 14-15 23.028612708477517 25.27677803228574 26.206695185366797 25.48791407386995 16-17 22.476947259588847 28.058773530566672 26.775922599951247 22.688356609893233 18-19 22.147117190783852 27.34166597912024 28.68510585304732 21.82611097704859 20-21 23.120302886994875 26.55107965515045 28.50182814336503 21.82678931448965 22-23 22.95502276790765 26.423077417691292 28.20937778293956 22.4125220314615 24-25 22.594779275394224 26.47753360953636 28.51794628149656 22.409740833572855 26-27 22.530424303811657 26.716962644089442 28.264370910772392 22.48824214132651 28-29 22.403949877228122 26.85081439170937 28.357644095012706 22.3875916360498 30-31 22.944797862710473 26.594382859650555 28.304424647683994 22.156394629954978 32-33 22.713535005721006 26.782407450263367 28.124295882351262 22.37976166166436 34-35 22.871990636841865 26.798227244728594 28.206478853083183 22.123303265346358 36-37 22.808685226897403 26.74061017706304 28.026779337853796 22.42392525818576 38-39 22.734606504763132 26.81925809392566 28.09653265441532 22.34960274689588 40-41 22.866771909572236 26.450897358742026 28.303248895134647 22.379081836551087 42-43 22.67990130818253 26.588795286941757 28.020245004043264 22.711058400832442 44-45 22.608247294214877 26.860004104675124 28.032043490138896 22.499705110971107 46-47 22.580525051768454 26.6760295375338 27.745995185710086 22.997450224987656 48-49 22.714975156860447 26.65432508645128 27.843293593451712 22.787406163236557 50-51 22.71275101117579 26.83838326971652 27.646373091026465 22.802492628081225 52-53 22.6126376589354 26.9990308290453 27.523819509488888 22.864512002530404 54-55 22.533831126497898 26.858372547021897 27.68960132844481 22.918194998035396 56-57 22.725054220572176 26.90109544096805 27.446576491128916 22.927273847330863 58-59 22.55485359189385 26.859175198063127 27.723828711881193 22.862142498161823 60-61 22.729033224643167 26.99856915689945 27.523173263119904 22.74922435533748 62-63 22.683881124856534 26.87232504529517 27.54627914035135 22.89751468949695 64-65 22.48975566988048 26.7473185339242 27.555114958936354 23.20781083725896 66-67 22.556770051018287 26.679672112246628 27.491274768528157 23.27228306820693 68-69 22.388202664021122 26.82045096569089 27.590443409994275 23.20090296029371 70-71 22.507655816739224 26.580502279229425 27.60729153238043 23.30455037165092 72-73 22.56771706891221 26.835613231318874 27.3916608471874 23.20500885258152 74-75 22.627213476000886 26.932615892200477 27.255800677495074 23.18436995430356 76-77 22.508983915323707 26.85123801185498 27.28445058551328 23.355327487308024 78-79 22.471280209904148 26.747559716164176 27.37325247531591 23.407907598615772 80-81 22.47759356213592 27.03135805289023 27.198522395727977 23.29252598924587 82-83 22.83266553273174 26.833294050945007 27.367894829286747 22.966145587036515 84-85 22.85261402776375 26.9147869596423 27.071274214613638 23.16132479798031 86-87 22.50935507353823 27.036791810161503 27.327924170771695 23.125928945528567 88-89 22.661770896220165 26.67605843435139 27.332005794008996 23.33016487541945 90-91 22.888616064055224 26.718706363180463 27.239435322663468 23.15324225010085 92-93 22.87317828609977 26.798359764899033 27.19675648799252 23.13170546100868 94-95 22.655469792987244 26.71592986761082 27.247346570382657 23.381253769019285 96-97 22.735628371938983 26.677435082314965 27.20034323323849 23.386593312507568 98-99 22.70363214085331 26.69871127303103 27.05013175389834 23.547524832217317 100 22.977415026161744 26.62683863849559 26.794008989600403 23.60173734574226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1517.0 1 1187.0 2 2005.5 3 2735.0 4 1776.5 5 1365.5 6 1821.0 7 3018.0 8 4921.5 9 6078.0 10 5658.5 11 5145.0 12 5454.0 13 6014.0 14 6693.0 15 7482.5 16 8192.5 17 8949.0 18 9710.0 19 10457.0 20 11563.5 21 12984.0 22 14920.0 23 17827.5 24 21651.5 25 26554.0 26 33322.0 27 41831.0 28 51994.0 29 65936.5 30 84196.0 31 104739.0 32 130082.5 33 158967.0 34 188388.0 35 217228.0 36 250449.5 37 285071.5 38 305614.5 39 315676.5 40 320114.0 41 323206.5 42 331176.0 43 345186.0 44 360114.5 45 378427.5 46 395253.5 47 404787.0 48 412445.0 49 414800.0 50 408953.5 51 397522.0 52 382207.5 53 364675.0 54 346238.0 55 319320.0 56 287726.5 57 252352.5 58 217213.5 59 186959.5 60 155509.0 61 125789.5 62 100857.0 63 79561.0 64 59863.0 65 43821.0 66 33214.0 67 25870.0 68 19982.5 69 15193.5 70 11932.0 71 9312.0 72 7286.5 73 5775.0 74 4467.5 75 3411.0 76 2491.0 77 1684.5 78 1116.0 79 756.0 80 543.5 81 356.0 82 239.0 83 174.0 84 127.0 85 105.5 86 85.5 87 62.5 88 49.0 89 43.5 90 35.0 91 31.5 92 30.0 93 22.5 94 16.0 95 11.5 96 8.5 97 5.5 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02466754826977055 2 0.11093394578741095 3 0.017055218896982475 4 0.017505356639131574 5 0.01955598413114413 6 0.04572399154141167 7 0.04633417825854712 8 0.03855179684983605 9 0.032930076603440654 10-11 0.03677125200311295 12-13 0.03588598111021973 14-15 0.031474631237158573 16-17 0.03769153360928444 18-19 0.036766250472644625 20-21 0.046999381810834115 22-23 0.04696937262802418 24-25 0.06585515167641298 26-27 0.05751760038571802 28-29 0.05036041028554738 30-31 0.04846483023805284 32-33 0.04381840843298049 34-35 0.04586403439452472 36-37 0.04087750851760639 38-39 0.045713988480475025 40-41 0.054526685165660685 42-43 0.056372249908472 44-45 0.05895303963012682 46-47 0.06060354468467351 48-49 0.06054852784952195 50-51 0.05479176628048183 52-53 0.0644447200843458 54-55 0.05107562914251761 56-57 0.05577206625227319 58-59 0.05367142345557741 60-61 0.049305087356731156 62-63 0.05534193463199739 64-65 0.054391643843015966 66-67 0.05229600257678849 68-69 0.05804776261536029 70-71 0.05845788811376281 72-73 0.0607085768245083 74-75 0.05134571178780707 76-77 0.05195589850494251 78-79 0.06095865334792447 80-81 0.061748895161919545 82-83 0.05186587095651269 84-85 0.05712748100918881 86-87 0.05438164078207931 88-89 0.06277420890792582 90-91 0.06812084497856344 92-93 0.07276726678363579 94-95 0.07606327536226086 96-97 0.07233713516335999 98-99 0.08053964513141021 100 0.12517830456119572 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9996940.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.98313266894007 #Duplication Level Percentage of deduplicated Percentage of total 1 74.74153810456151 44.83231596006553 2 14.976609683448515 17.966879311464503 3 4.90824996489122 8.832366264491714 4 2.088776203036295 5.01165360409804 5 1.0557335825871788 3.166310377369107 6 0.5904570807440941 2.125047924575284 7 0.3575663569131456 1.501356515726955 8 0.2430117106412618 1.1661282943600708 9 0.17568960751897494 0.9484571732728218 >10 0.7951285563698214 8.21060335247546 >50 0.0417601192795057 1.733756478240665 >100 0.02361359268541856 2.623213795563218 >500 0.0013588228694227356 0.5549358335077602 >1k 4.375306664196163E-4 0.5141565895658501 >5k 0.0 0.0 >10k+ 6.908378713545967E-5 0.812818525223095 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27443 0.274514001284393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19784 0.19790055757061661 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11618 0.11621556196196037 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0006121873293227E-5 0.0 4 0.0 0.0 0.0 2.0006121873293227E-5 1.0003060936646614E-5 5 0.0 0.0 0.0 2.0006121873293227E-5 1.0003060936646614E-5 6 0.0 0.0 0.0 2.0006121873293227E-5 2.0006121873293227E-5 7 0.0 1.0003060936646614E-5 0.0 2.0006121873293227E-5 3.000918280993984E-5 8 0.0 1.0003060936646614E-5 0.0 2.0006121873293227E-5 3.000918280993984E-5 9 0.0 1.0003060936646614E-5 0.0 6.001836561987968E-5 4.0012243746586455E-5 10-11 0.0 1.0003060936646614E-5 5.001530468323307E-6 6.001836561987968E-5 4.0012243746586455E-5 12-13 0.0 1.500459140496992E-5 1.0003060936646614E-5 8.002448749317291E-5 6.501989608820298E-5 14-15 0.0 2.5007652341616536E-5 1.0003060936646614E-5 1.1003367030311276E-4 1.0003060936646613E-4 16-17 5.001530468323307E-6 3.000918280993984E-5 1.0003060936646614E-5 1.2003673123975936E-4 1.4004285311305258E-4 18-19 1.0003060936646614E-5 4.0012243746586455E-5 1.0003060936646614E-5 1.500459140496992E-4 1.500459140496992E-4 20-21 1.0003060936646614E-5 5.001530468323307E-5 1.0003060936646614E-5 2.3507193201119543E-4 1.550474445180225E-4 22-23 2.0006121873293227E-5 5.001530468323307E-5 1.0003060936646614E-5 6.602020218186766E-4 2.000612187329323E-4 24-25 4.501377421490976E-5 5.501683515155638E-5 1.0003060936646614E-5 0.0019155861693678266 2.5007652341616535E-4 26-27 6.001836561987968E-5 6.001836561987968E-5 1.0003060936646614E-5 0.0034160453098648186 2.6508111482113526E-4 28-29 6.001836561987968E-5 6.001836561987968E-5 1.0003060936646614E-5 0.006977135003311013 2.700826452894586E-4 30-31 7.50229570248496E-5 6.001836561987968E-5 1.0003060936646614E-5 0.01780044693676265 2.8008570622610517E-4 32-33 8.002448749317291E-5 6.001836561987968E-5 1.0003060936646614E-5 0.04381340690251217 3.1009488903604505E-4 34-35 9.002754842981953E-5 6.501989608820298E-5 1.0003060936646614E-5 0.0838856690147185 3.1009488903604505E-4 36-37 1.0503213983478944E-4 8.002448749317291E-5 1.0003060936646614E-5 0.14855545797013886 3.1509641950436837E-4 38-39 1.1003367030311275E-4 9.502907889814284E-5 1.0003060936646614E-5 0.2426842613839835 3.2009794997269164E-4 40-41 1.500459140496992E-4 1.0003060936646614E-4 1.0003060936646614E-5 0.34866669200775435 3.2009794997269164E-4 42-43 1.500459140496992E-4 1.1003367030311275E-4 1.0003060936646614E-5 0.4549692205814979 3.2009794997269164E-4 44-45 1.550474445180225E-4 1.1003367030311275E-4 1.0003060936646614E-5 0.5837686332017598 3.2509948044101496E-4 46-47 1.6004897498634582E-4 1.2003673123975936E-4 1.0003060936646614E-5 0.7348598671193385 3.451056023143082E-4 48-49 1.6004897498634582E-4 1.2003673123975936E-4 1.0003060936646614E-5 0.8946937762955465 4.1012549840251116E-4 50-51 1.6004897498634582E-4 1.2003673123975936E-4 1.0003060936646614E-5 1.063850538264709 4.1012549840251116E-4 52-53 1.7505356639131573E-4 1.2503826170808267E-4 1.0003060936646614E-5 1.2311517324301238 4.301316202758044E-4 54-55 1.8005509685963905E-4 1.3003979217640597E-4 1.0003060936646614E-5 1.41834401326806 4.5013774214909763E-4 56-57 1.8005509685963905E-4 1.3003979217640597E-4 1.0003060936646614E-5 1.6334898478934554 4.601408030857442E-4 58-59 1.9005815779628567E-4 1.3003979217640597E-4 1.0003060936646614E-5 1.8741434879072996 4.7014386402239087E-4 60-61 2.100642796695789E-4 1.3504132264472926E-4 1.0003060936646614E-5 2.121734250680708 4.7014386402239087E-4 62-63 2.100642796695789E-4 1.4004285311305258E-4 1.0003060936646614E-5 2.375556920417648 5.101561077689773E-4 64-65 2.100642796695789E-4 1.4004285311305258E-4 1.0003060936646614E-5 2.633950989002635 5.101561077689773E-4 66-67 2.150658101379022E-4 1.4004285311305258E-4 1.0003060936646614E-5 2.923654638319326 5.151576382373006E-4 68-69 2.200673406062255E-4 1.4004285311305258E-4 1.0003060936646614E-5 3.2501945595352177 5.201591687056239E-4 70-71 2.200673406062255E-4 1.450443835813759E-4 1.0003060936646614E-5 3.5922442267333805 5.40165290578917E-4 72-73 2.300704015428721E-4 1.500459140496992E-4 1.0003060936646614E-5 3.930477726184212 5.951821257304735E-4 74-75 2.3507193201119543E-4 1.500459140496992E-4 1.0003060936646614E-5 4.264584962998677 6.001836561987968E-4 76-77 2.4007346247951873E-4 1.6004897498634582E-4 1.0003060936646614E-5 4.6304769259393375 6.101867171354435E-4 78-79 2.4007346247951873E-4 1.6004897498634582E-4 1.0003060936646614E-5 5.0302842669856975 6.201897780720901E-4 80-81 2.4007346247951873E-4 1.6004897498634582E-4 1.0003060936646614E-5 5.442065271973224 6.201897780720901E-4 82-83 2.45074992947842E-4 1.6004897498634582E-4 1.0003060936646614E-5 5.8693710275344255 6.501989608820299E-4 84-85 2.5007652341616535E-4 1.6004897498634582E-4 1.0003060936646614E-5 6.294986265797334 6.501989608820299E-4 86-87 2.5007652341616535E-4 1.6004897498634582E-4 1.0003060936646614E-5 6.73555608016053 6.501989608820299E-4 88 2.5007652341616535E-4 1.6004897498634582E-4 1.0003060936646614E-5 7.078916148341392 6.501989608820299E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13780 0.0 47.140793 1 GTATCAA 25600 0.0 43.437008 1 ATCAACG 31290 0.0 34.447826 3 CAACGCA 31460 0.0 34.186665 5 TATCAAC 32470 0.0 33.767704 2 TCAACGC 32425 0.0 33.270336 4 AACGCAG 33620 0.0 32.466892 6 ACGCAGA 37950 0.0 28.45353 7 CGCAGAG 39165 0.0 27.450302 8 TACCTGG 7595 0.0 26.816473 2 GTACATG 35455 0.0 25.4197 1 GCAGAGT 43830 0.0 24.13417 9 TACATGG 36635 0.0 23.958355 2 GTACCTG 9210 0.0 22.982924 1 ACATGGG 36875 0.0 22.974058 3 GTGGTAT 7260 0.0 21.834623 1 CATGGGG 26080 0.0 21.240831 4 GTACTAG 3310 0.0 20.74805 1 GAGTACT 24110 0.0 20.35775 12-13 AGAGTAC 40400 0.0 20.1525 10-11 >>END_MODULE