FastQCFastQC Report
Mon 6 Jun 2016
ERR1211284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9996940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242850.24292433484646303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187270.18732732216058112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111530.11156413862641967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139600.048.813651
GTATCAA260500.042.1928861
ATCAACG313150.034.4180343
CAACGCA317150.033.9456945
TATCAAC327550.033.3176842
TCAACGC326350.033.0834274
AACGCAG339800.032.0419166
ACGCAGA386000.027.9637897
CGCAGAG397550.027.068768
TACCTGG79850.026.7936952
GCAGAGT446050.024.0199839
GTACATG358300.023.655441
TACATGG365750.022.7953262
GTACCTG99000.022.0433621
ACATGGG363400.022.0407373
ACCTGGG99550.021.3046633
GAGTACT245650.020.98434612-13
GTGGTAT73750.020.2360231
AGAGTAC414100.019.86198810-11
CAGAGTA435950.019.65192810-11
Produced by FastQC (version 0.11.5)