##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9996940 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11136047630575 33.0 31.0 34.0 30.0 34.0 2 32.23989310729083 34.0 31.0 34.0 30.0 34.0 3 32.30967516059914 34.0 31.0 34.0 30.0 34.0 4 35.94721834881474 37.0 35.0 37.0 35.0 37.0 5 35.850660302052425 37.0 35.0 37.0 35.0 37.0 6 35.86333988200389 37.0 35.0 37.0 35.0 37.0 7 35.785101741132785 37.0 35.0 37.0 33.0 37.0 8 35.76430927863927 37.0 35.0 37.0 33.0 37.0 9 37.384713622368444 39.0 37.0 39.0 34.0 39.0 10-11 37.33423117473947 39.0 37.0 39.0 34.0 39.0 12-13 37.26753296508732 39.0 37.0 39.0 34.0 39.0 14-15 38.5103880787521 40.0 38.0 41.0 34.0 41.0 16-17 38.49299045507925 40.0 38.0 41.0 34.0 41.0 18-19 38.4946922258211 40.0 38.0 41.0 34.0 41.0 20-21 38.46454980223949 40.0 38.0 41.0 34.0 41.0 22-23 38.357108475193414 40.0 38.0 41.0 34.0 41.0 24-25 38.26747479728797 40.0 38.0 41.0 34.0 41.0 26-27 38.17100037611509 40.0 38.0 41.0 33.0 41.0 28-29 38.07000607185799 40.0 37.5 41.0 33.0 41.0 30-31 38.08400795643467 40.0 37.0 41.0 33.0 41.0 32-33 38.0277296852837 40.0 37.0 41.0 33.0 41.0 34-35 37.89994303256796 40.0 37.0 41.0 33.0 41.0 36-37 37.75430206643233 40.0 37.0 41.0 33.0 41.0 38-39 37.64415786230587 40.0 37.0 41.0 33.0 41.0 40-41 37.542794195023674 40.0 37.0 41.0 32.0 41.0 42-43 37.44602448349195 40.0 36.5 41.0 32.0 41.0 44-45 37.306057253519576 40.0 36.0 41.0 31.5 41.0 46-47 37.088914507839405 39.5 36.0 41.0 31.0 41.0 48-49 35.55644102095241 39.0 35.0 41.0 24.5 41.0 50-51 36.309707770577795 39.0 35.0 40.0 29.0 41.0 52-53 36.41030090207603 39.0 35.0 40.0 30.0 41.0 54-55 36.25463726900431 39.0 35.0 40.0 29.0 41.0 56-57 36.04743831612473 38.0 35.0 40.0 29.0 41.0 58-59 35.819997069103145 38.0 34.5 40.0 28.0 41.0 60-61 35.571026284042915 38.0 34.0 40.0 28.0 41.0 62-63 35.25774532006794 37.0 34.0 40.0 28.0 41.0 64-65 34.929828227437596 37.0 34.0 39.5 27.5 41.0 66-67 34.59465941578123 36.0 33.5 39.0 26.5 40.5 68-69 34.23708559819305 36.0 33.0 39.0 26.0 40.0 70-71 33.87323480985181 35.0 33.0 38.5 26.0 40.0 72-73 33.48948468231279 35.0 32.0 37.5 26.0 40.0 74-75 33.120768155055444 35.0 32.0 37.0 26.0 39.0 76-77 32.17198382705108 34.0 30.5 36.0 24.5 38.5 78-79 32.60092948442224 35.0 32.0 36.0 26.0 38.5 80-81 32.46245075993254 35.0 32.0 36.0 25.0 37.5 82-83 32.18134409129193 34.5 31.5 35.0 25.0 37.0 84-85 31.891948136129656 34.0 31.0 35.0 24.5 36.5 86-87 31.62605557300534 34.0 31.0 35.0 24.0 36.0 88-89 31.390732464134025 34.0 31.0 35.0 24.0 36.0 90-91 31.166350453238692 34.0 31.0 35.0 23.0 35.0 92-93 30.97394517722423 34.0 30.0 35.0 23.0 35.0 94-95 30.777069933399623 34.0 30.0 35.0 20.5 35.0 96-97 30.585551178660673 34.0 30.0 35.0 20.0 35.0 98-99 30.36021282512449 34.0 29.0 35.0 20.0 35.0 100 27.924204406548405 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29152505768072245 1101 2 -0.27265959001133666 1101 3 -0.3068427327638581 1101 4 -0.1974601019292237 1101 5 -0.21106800689574357 1101 6 -0.28510528998245377 1101 7 -0.27461066535772005 1101 8 -0.2946687068311249 1101 9 -0.3786244441344806 1101 10-11 -0.35158620821125197 1101 12-13 -0.41650489377064304 1101 14-15 -0.5479030145721069 1101 16-17 -0.5916970867462226 1101 18-19 -0.5125519143158215 1101 20-21 -0.536206564506216 1101 22-23 -0.6552863884935931 1101 24-25 -0.6644575660459324 1101 26-27 -0.6461835265971843 1101 28-29 -0.6593073160182357 1101 30-31 -0.5839726958392149 1101 32-33 -0.5131807975961351 1101 34-35 -0.6261438242005752 1101 36-37 -0.629191214389607 1101 38-39 -0.6312006631579195 1101 40-41 -0.5635934532661295 1101 42-43 -0.6116007282177733 1101 44-45 -0.5691455356567943 1101 46-47 -0.6131801387862694 1101 48-49 0.6223299518827616 1101 50-51 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0.3485074854434913 2311 2 0.3821472160783159 2311 3 0.37992467400540164 2311 4 0.28435123896181125 2311 5 0.3197474768246025 2311 6 0.3530919760374971 2311 7 0.3969483156394702 2311 8 0.20442267539879566 2311 9 0.41439709695060856 2311 10-11 0.387986234846565 2311 12-13 0.4895996844168806 2311 14-15 0.4352648540451298 2311 16-17 0.4481335234594539 2311 18-19 0.5914214538844718 2311 20-21 0.6484507979637755 2311 22-23 0.6579318950444559 2311 24-25 0.6120945333401764 2311 26-27 0.6786067226405947 2311 28-29 0.7288341276343644 2311 30-31 0.5989939179249504 2311 32-33 0.6053160899829209 2311 34-35 0.7479084085384144 2311 36-37 0.7745917028007199 2311 38-39 0.7874418327016102 2311 40-41 0.7067908973445469 2311 42-43 0.7699401951426452 2311 44-45 0.8057310237807727 2311 46-47 0.8720407864948356 2311 48-49 -6.173377014742655 2311 50-51 -1.105345132785537 2311 52-53 0.3417061355777946 2311 54-55 0.8438463778212224 2311 56-57 0.9603379565080985 2311 58-59 0.9914488186425601 2311 60-61 0.8705014093137535 2311 62-63 0.7248716430678002 2311 64-65 0.8000674587478613 2311 66-67 0.9382531048535938 2311 68-69 0.9457869127897709 2311 70-71 0.9527354716350729 2311 72-73 0.43853498671096247 2311 74-75 0.740023867405263 2311 76-77 0.7898217504041725 2311 78-79 0.7369825134560131 2311 80-81 0.7519402844227869 2311 82-83 0.7625629500120183 2311 84-85 0.810323667861006 2311 86-87 0.9061096840314491 2311 88-89 0.897637470274443 2311 90-91 0.963794467526423 2311 92-93 0.8498833962920322 2311 94-95 0.9072320245384837 2311 96-97 1.016211922042018 2311 98-99 1.049863627712881 2311 100 1.167367026921383 2312 1 0.08141925675232642 2312 2 0.21171856268924927 2312 3 0.17279624903667212 2312 4 -0.0061652190619412295 2312 5 0.2060180248552399 2312 6 0.14605548936320645 2312 7 0.27602308855544067 2312 8 0.36280705586653994 2312 9 0.26159386984520694 2312 10-11 0.23654177020895162 2312 12-13 0.3023026702733489 2312 14-15 0.3400598993550261 2312 16-17 0.4455608547901022 2312 18-19 0.2961936927957538 2312 20-21 0.37075848478915674 2312 22-23 0.6354848753775002 2312 24-25 0.6769937041137766 2312 26-27 0.6691575684372708 2312 28-29 0.741275730781652 2312 30-31 0.7669801485617853 2312 32-33 0.7697217160363792 2312 34-35 0.3761698126285964 2312 36-37 0.7086619678894692 2312 38-39 0.7895544535488028 2312 40-41 0.5057182839094381 2312 42-43 0.5630337762104887 2312 44-45 0.7098197834333959 2312 46-47 0.782042104989749 2312 48-49 -4.800851776255435 2312 50-51 -0.8259526738637888 2312 52-53 0.5047269856150365 2312 54-55 0.7517694632418781 2312 56-57 0.8612338203538954 2312 58-59 0.88851196029524 2312 60-61 0.9271726372601492 2312 62-63 0.717119961986775 2312 64-65 0.9134036785160191 2312 66-67 0.8630390276040387 2312 68-69 0.7881134505290106 2312 70-71 0.8324297589885674 2312 72-73 0.9853957215758911 2312 74-75 0.6263998950295075 2312 76-77 0.8120180778612891 2312 78-79 0.47675526907475785 2312 80-81 0.6812037446523433 2312 82-83 0.7050129630187953 2312 84-85 0.7582755463599185 2312 86-87 0.846253221802769 2312 88-89 0.8287368455929318 2312 90-91 0.6530305600776423 2312 92-93 0.8267076409191674 2312 94-95 0.9235704928750046 2312 96-97 1.000338134077385 2312 98-99 1.0241321302946353 2312 100 0.6368762973660722 2313 1 0.06304714990164939 2313 2 0.21217060674901234 2313 3 0.25628371617399637 2313 4 0.06038992980921165 2313 5 0.11617483042720522 2313 6 0.23587583215401509 2313 7 0.15600855470348307 2313 8 0.23072202688830146 2313 9 0.4290867394496729 2313 10-11 0.23981768069621268 2313 12-13 0.006678101168169803 2313 14-15 0.11207443829349018 2313 16-17 0.07105472893358211 2313 18-19 0.38881576895811776 2313 20-21 0.4045865225847578 2313 22-23 0.4497157298251224 2313 24-25 0.25608711442846044 2313 26-27 0.37908416012979274 2313 28-29 0.4015623851200232 2313 30-31 0.5812202429865252 2313 32-33 0.46354567996519336 2313 34-35 0.368447576995365 2313 36-37 -0.34983639120947885 2313 38-39 0.17017803492450412 2313 40-41 0.4376365824666735 2313 42-43 0.5229942503272582 2313 44-45 0.5399536542986425 2313 46-47 0.3821551351919439 2313 48-49 -5.236043735500935 2313 50-51 -1.3122068384561558 2313 52-53 0.13266311814074072 2313 54-55 0.31705649673534 2313 56-57 0.3741832269193779 2313 58-59 0.40481389090418674 2313 60-61 0.7080799017346706 2313 62-63 0.7850906445024961 2313 64-65 0.859439036838495 2313 66-67 0.8560553881874355 2313 68-69 0.9058247583091799 2313 70-71 0.9004540228374083 2313 72-73 0.6573489794511147 2313 74-75 0.7798479429797496 2313 76-77 0.7839718917905216 2313 78-79 0.7389167155230467 2313 80-81 -0.16987952076941326 2313 82-83 0.27506738828988375 2313 84-85 0.6211456403628404 2313 86-87 0.7532854327994158 2313 88-89 0.7696231062751657 2313 90-91 0.7890462286710225 2313 92-93 0.8022682513240937 2313 94-95 0.7318924346258022 2313 96-97 0.8180360303467786 2313 98-99 0.9370376183760349 2313 100 0.97886012286137 2314 1 0.051347791979189594 2314 2 0.1541116926836068 2314 3 0.21110832563701365 2314 4 0.04551032157488777 2314 5 0.1643095891566304 2314 6 0.24062372612170435 2314 7 0.09236200280144402 2314 8 0.2458807445114246 2314 9 0.38922770629896775 2314 10-11 0.31784094385284334 2314 12-13 0.18783149911967456 2314 14-15 0.23786163142580108 2314 16-17 0.4357880428106853 2314 18-19 0.5184551312285564 2314 20-21 0.5342297477697855 2314 22-23 0.31645239761062527 2314 24-25 0.40350765302319047 2314 26-27 0.44430098129119244 2314 28-29 0.5805422362900643 2314 30-31 0.504516093356024 2314 32-33 0.4652137299407215 2314 34-35 0.34750576689500434 2314 36-37 0.4515157666234444 2314 38-39 0.48882951743747327 2314 40-41 0.24764928434851896 2314 42-43 0.31058567896093336 2314 44-45 0.479208850927769 2314 46-47 0.6045330973974856 2314 48-49 -6.423277364708241 2314 50-51 -1.5417301947130895 2314 52-53 -0.8981079278612611 2314 54-55 -0.4862218561408582 2314 56-57 -0.12356629574738065 2314 58-59 0.33401718719512274 2314 60-61 0.5903432840749971 2314 62-63 0.5109225642202162 2314 64-65 0.6662953250587975 2314 66-67 0.6435817732934694 2314 68-69 0.6407589384424881 2314 70-71 0.450844274428853 2314 72-73 0.6327998625988229 2314 74-75 0.6417807395194259 2314 76-77 0.5676812901092703 2314 78-79 0.37921678672883274 2314 80-81 0.4528807344660635 2314 82-83 0.44484473107099376 2314 84-85 0.5109244721888899 2314 86-87 0.5957941690624864 2314 88-89 0.4844987322827592 2314 90-91 0.6376686882854514 2314 92-93 0.7077502213230389 2314 94-95 0.785757449704132 2314 96-97 0.8012200892199246 2314 98-99 0.9065033590960567 2314 100 -0.5112275057175282 2315 1 0.13171115716512816 2315 2 0.21576349022110008 2315 3 0.3161480593951822 2315 4 0.13771309950787725 2315 5 0.22800942221132914 2315 6 0.2856496388453351 2315 7 0.34300985485717206 2315 8 0.3085319943072591 2315 9 0.46856631365083246 2315 10-11 0.18744035751857524 2315 12-13 0.3740022994427292 2315 14-15 0.21089477575219462 2315 16-17 0.3446813094982346 2315 18-19 0.4450570846473809 2315 20-21 0.3638630696199172 2315 22-23 0.4310183935439085 2315 24-25 0.45098232174145636 2315 26-27 0.4612388168537507 2315 28-29 0.5729160624677974 2315 30-31 0.528296645262408 2315 32-33 0.6166006149253107 2315 34-35 0.6249290095922433 2315 36-37 0.5464029555967187 2315 38-39 0.6573864572209729 2315 40-41 0.6891475805088803 2315 42-43 0.6896553593331376 2315 44-45 0.7324811214325493 2315 46-47 0.626889218538814 2315 48-49 -4.749185579247069 2315 50-51 -0.8346554443305294 2315 52-53 0.29584813748338945 2315 54-55 0.18596757783812023 2315 56-57 0.5275287563854718 2315 58-59 0.6869768870039294 2315 60-61 0.602338902799616 2315 62-63 0.3074673048713876 2315 64-65 0.48188357687054406 2315 66-67 -0.188468549235715 2315 68-69 0.5169950521776272 2315 70-71 0.5109705511823819 2315 72-73 0.6520717317324269 2315 74-75 0.7736513266764362 2315 76-77 0.6823326628648942 2315 78-79 0.6176800147955959 2315 80-81 0.44300110234263457 2315 82-83 0.41873534042282046 2315 84-85 0.48845802259863547 2315 86-87 0.5132556112377529 2315 88-89 0.5759789856176702 2315 90-91 0.6580689365446624 2315 92-93 0.6553988413840415 2315 94-95 0.233083143017911 2315 96-97 0.36066972247927964 2315 98-99 0.3783454824499515 2315 100 0.4665392828730539 2316 1 0.202277521211208 2316 2 0.23331607104042007 2316 3 0.13722461189811241 2316 4 -0.04922771573747298 2316 5 0.11425807394655152 2316 6 0.11113817910712243 2316 7 0.22383084411759313 2316 8 0.23967879764471434 2316 9 0.3747118932111775 2316 10-11 0.3819330229340565 2316 12-13 0.299264181715607 2316 14-15 0.41035820676022183 2316 16-17 0.40287034363546326 2316 18-19 0.12195515088664166 2316 20-21 0.4420621254668333 2316 22-23 0.5114529827177208 2316 24-25 0.5601344986058265 2316 26-27 0.5944464728997971 2316 28-29 0.6088945066287152 2316 30-31 0.5776732182379831 2316 32-33 0.5487174575466085 2316 34-35 0.5284773483057776 2316 36-37 0.4104105736393677 2316 38-39 0.4840008121385253 2316 40-41 0.046155133203029663 2316 42-43 0.0461354821786486 2316 44-45 0.33349385426065936 2316 46-47 0.3468328910324274 2316 48-49 -5.10839116825521 2316 50-51 -1.064060903268647 2316 52-53 0.22054298293420516 2316 54-55 0.5457493066409214 2316 56-57 0.386494714161671 2316 58-59 0.23780022977461357 2316 60-61 0.428467785940839 2316 62-63 0.32974390647008534 2316 64-65 0.3242060453941491 2316 66-67 0.40372575425666213 2316 68-69 0.17083625341312825 2316 70-71 -0.2639438025053593 2316 72-73 -0.15743548517657047 2316 74-75 0.10631427531131266 2316 76-77 0.1147133108373879 2316 78-79 0.1798323385132079 2316 80-81 0.15155562987182947 2316 82-83 0.12251261588262707 2316 84-85 0.27254642439878296 2316 86-87 0.34402351389117314 2316 88-89 0.09436060007266178 2316 90-91 0.16312144803671202 2316 92-93 0.28048682673826164 2316 94-95 0.53109529494375 2316 96-97 0.5917644761046148 2316 98-99 0.5727430187876905 2316 100 -0.3053029682788022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 8.0 11 20.0 12 49.0 13 202.0 14 834.0 15 2438.0 16 5545.0 17 11483.0 18 19359.0 19 28123.0 20 39171.0 21 53466.0 22 67076.0 23 73233.0 24 78021.0 25 88192.0 26 105059.0 27 129332.0 28 160751.0 29 197546.0 30 242960.0 31 301563.0 32 379820.0 33 492908.0 34 681215.0 35 958115.0 36 1490553.0 37 2255710.0 38 1890463.0 39 243699.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33587992184129 19.723696881256238 14.091961356106408 23.84846184079606 2 15.488133643434116 20.877225098156902 39.97991347187836 23.65472778653062 3 18.62155681794311 26.077835895619955 29.98700601084533 25.31360127559161 4 12.885763228165285 17.315203691829044 38.39062179052789 31.40841128947779 5 13.571275738351316 37.38420536524269 34.52848037606854 14.516038520337451 6 30.445175357211912 37.082398338611114 18.493754637045328 13.978671667131643 7 27.43312728026049 32.204137686545856 22.387672866664484 17.975062166529174 8 25.750349320725242 35.22070227470614 20.516870630489898 18.512077774078723 9 26.266438827109813 16.534841254779572 20.65967294866117 36.539046969449444 10-11 24.79373721782944 26.315328801565695 28.28402189292911 20.60691208767575 12-13 25.473772900903285 23.792842023216927 28.390317988590695 22.34306708728909 14-15 22.875728142617447 25.396688211790714 26.1378610853907 25.589722560201132 16-17 22.401281556834075 28.19827659634806 26.72940676080348 22.671035086014385 18-19 22.065955492273957 27.458156314555705 28.708719174375236 21.767169018795105 20-21 23.029449283567736 26.596998629329548 28.525815293841383 21.847736793261333 22-23 22.84439564273709 26.458679565041948 28.256878770036923 22.44004602218404 24-25 22.518835267358867 26.556906724839145 28.5323315999359 22.391926407866087 26-27 22.496408523673782 26.7641772349491 28.300923478184586 22.438490763192533 28-29 22.342414671519133 26.89318862624362 28.414389630001008 22.35000707223624 30-31 22.85021069262904 26.649480279511035 28.381016621382425 22.119292406477495 32-33 22.70966838992488 26.789870775142067 28.13264963229744 22.367811202635618 34-35 22.811900536156134 26.838806840871847 28.252305285289196 22.09698733768282 36-37 22.73689246811516 26.787539424735908 28.101618080657754 22.373950026491183 38-39 22.67178714580179 26.8383729378729 28.20049289147323 22.28934702485208 40-41 22.804913646655432 26.461253484474444 28.393572501376983 22.340260367493137 42-43 22.61334190703766 26.578306694061705 28.142100746294414 22.66625065260622 44-45 22.511389832410913 26.86718447264337 28.10290307111688 22.518522623828837 46-47 22.5109754973307 26.679285981291024 27.817370285495734 22.992368235882545 48-49 22.758075048736153 26.747804905139734 27.80846008040079 22.685659965723325 50-51 22.654804970086882 26.832416267912667 27.691994080156995 22.820784681843456 52-53 22.519703969968386 26.99948384538093 27.58063552750572 22.900176657144968 54-55 22.48072752366424 26.878155452520915 27.70872342722197 22.932393596592874 56-57 22.693928831867513 26.873393103871194 27.45116418797653 22.981513876284758 58-59 22.44967026905331 26.892400934784146 27.734493972126845 22.923434824035706 60-61 22.665462168368034 27.04017984844869 27.525153638806465 22.769204344376806 62-63 22.62505503352831 26.92542320420633 27.493730354368406 22.955791407896957 64-65 22.37632144782748 26.775104519728508 27.554248858154416 23.294325174289593 66-67 22.409307320193083 26.72399491456196 27.493884846807482 23.372812918437475 68-69 22.268632964792936 26.816493278024673 27.58397572457284 23.330898032609547 70-71 22.33440848036059 26.581197547560148 27.630091256088974 23.454302715990288 72-73 22.380299384922335 26.820635940948662 27.3977230585162 23.401341615612804 74-75 22.417374620292073 26.932723276312725 27.227736119192812 23.42216598420239 76-77 22.256686575266322 26.830603891966327 27.301445158442593 23.611264374324758 78-79 22.20900317988968 26.739669460139037 27.375642394187373 23.675684965783912 80-81 22.135933479442002 27.035955676835954 27.202426128143287 23.62568471557876 82-83 22.453014563778215 26.817636005339395 27.356022337012565 23.37332709386983 84-85 22.492158955459907 26.88675087345339 27.07344312479945 23.547647046287253 86-87 22.104796643028745 26.975769776582954 27.321121110895387 23.598312469492917 88-89 22.161528733446314 26.672279225871087 27.322261412074468 23.84393062860813 90-91 22.323257320888356 26.675512198674987 27.297325784865876 23.703904695570778 92-93 22.24267727423657 26.767550096147026 27.23125778277685 23.758514846839553 94-95 21.949361751188377 26.644420096540745 27.338862213734632 24.06735593853624 96-97 21.967488406258276 26.630899018532077 27.334161151684526 24.067451423525117 98-99 21.881855795861714 26.63037037811466 27.230405164044875 24.257368661978752 100 21.804029770441712 26.61597525073619 27.284238194353204 24.295756784468892 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1473.0 1 1110.0 2 1670.0 3 2229.5 4 1429.0 5 1101.0 6 1523.5 7 2633.0 8 4225.0 9 5206.5 10 4921.0 11 4483.0 12 4799.0 13 5441.0 14 6217.5 15 6998.5 16 7810.5 17 8711.0 18 9603.0 19 10563.5 20 11678.0 21 13093.5 22 15072.5 23 18054.0 24 21996.0 25 26899.0 26 33773.5 27 42314.0 28 52486.0 29 66469.0 30 85188.5 31 105890.0 32 131332.5 33 160474.0 34 189160.5 35 217856.5 36 251318.5 37 285528.0 38 305686.0 39 316326.0 40 320613.0 41 323870.0 42 333209.5 43 347140.5 44 362869.0 45 381686.0 46 398387.5 47 408350.5 48 417577.0 49 419632.0 50 411729.5 51 400217.5 52 384863.5 53 366582.5 54 348373.0 55 320651.0 56 286609.5 57 251133.5 58 216492.0 59 185517.0 60 152572.0 61 121704.5 62 97015.0 63 75635.0 64 55840.0 65 40531.0 66 30243.5 67 23235.0 68 17787.5 69 13312.5 70 10247.0 71 8007.0 72 6267.0 73 4943.0 74 3847.5 75 2880.5 76 2023.0 77 1216.5 78 739.5 79 508.0 80 323.0 81 198.0 82 135.0 83 78.0 84 38.0 85 30.0 86 21.5 87 19.0 88 13.0 89 4.5 90 2.5 91 2.5 92 3.5 93 3.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029168925691261526 2 0.0019005815779628568 3 1.0003060936646614E-4 4 0.021116461637261004 5 3.9011937652921793E-4 6 3.000918280993984E-5 7 1.0003060936646614E-5 8 0.003961212130912059 9 0.010173112972569606 10-11 0.0010503213983478945 12-13 9.002754842981953E-5 14-15 1.450443835813759E-4 16-17 3.651117241876014E-4 18-19 1.500459140496992E-5 20-21 7.50229570248496E-5 22-23 2.200673406062255E-4 24-25 2.0006121873293227E-5 26-27 6.501989608820298E-5 28-29 0.0010203122155379547 30-31 0.002685821861489616 32-33 0.009532917072624223 34-35 0.018110541825798694 36-37 0.021051441741172797 38-39 0.020426250432632385 40-41 0.004541389665237563 42-43 0.0048314784324003145 44-45 0.008537612509427885 46-47 0.011518524668548576 48-49 1.2058139790775977 50-51 0.017430333682106725 52-53 0.018735733134339107 54-55 0.009492904828877637 56-57 0.00766734620793963 58-59 0.008357557412568247 60-61 0.0052366024003345025 62-63 0.007612329372788072 64-65 0.01937592903428449 66-67 0.029148919569388233 68-69 0.029664077207625535 70-71 0.02648810536024023 72-73 0.02349218860971457 74-75 0.009347860445296261 76-77 0.014274367956594718 78-79 0.008387566595378185 80-81 0.0027108295138312324 82-83 0.014229354182379809 84-85 0.024177398283874864 86-87 0.02336214881753817 88-89 0.03282504446360587 90-91 0.020641316242770286 92-93 0.013114012887943711 94-95 0.006426966651795449 96-97 0.003831172338735653 98-99 0.01519965109323453 100 0.03270000620189778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9996940.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.99044103142131 #Duplication Level Percentage of deduplicated Percentage of total 1 74.62708207447638 44.76911566535914 2 15.05285710465318 18.06055072982216 3 4.8791843236523755 8.781132583485094 4 2.0866196642864923 5.007089357015318 5 1.060220599797066 3.180155068621201 6 0.6244534776441238 2.247674371648547 7 0.38857277496612663 1.6317456500114815 8 0.25557871394952103 1.2265823814460193 9 0.1709839099983546 0.9231660153070327 >10 0.788399377293909 8.122479924638116 >50 0.04047960233675907 1.6602616115406876 >100 0.02357627016172293 2.5682823075312573 >500 0.0015288764789317597 0.6072707250341697 >1k 3.9374576026333154E-4 0.46188745454731467 >5k 0.0 0.0 >10k+ 6.948454494669038E-5 0.752606153992444 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24285 0.24292433484646303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18727 0.18732732216058112 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11153 0.11156413862641967 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0003060936646614E-5 2 0.0 0.0 0.0 0.0 1.0003060936646614E-5 3 0.0 0.0 0.0 0.0 1.0003060936646614E-5 4 0.0 0.0 0.0 0.0 2.0006121873293227E-5 5 0.0 0.0 0.0 0.0 2.0006121873293227E-5 6 0.0 0.0 0.0 0.0 2.0006121873293227E-5 7 0.0 0.0 0.0 0.0 2.0006121873293227E-5 8 1.0003060936646614E-5 0.0 0.0 0.0 2.0006121873293227E-5 9 1.0003060936646614E-5 0.0 0.0 2.0006121873293227E-5 2.0006121873293227E-5 10-11 3.000918280993984E-5 0.0 0.0 2.0006121873293227E-5 2.0006121873293227E-5 12-13 6.001836561987968E-5 0.0 0.0 4.0012243746586455E-5 2.0006121873293227E-5 14-15 6.001836561987968E-5 5.001530468323307E-6 0.0 7.002142655652629E-5 3.000918280993984E-5 16-17 6.001836561987968E-5 1.0003060936646614E-5 0.0 8.002448749317291E-5 4.0012243746586455E-5 18-19 6.001836561987968E-5 1.0003060936646614E-5 0.0 1.0503213983478944E-4 9.002754842981953E-5 20-21 6.001836561987968E-5 2.0006121873293227E-5 0.0 1.9005815779628564E-4 9.002754842981953E-5 22-23 6.501989608820298E-5 2.0006121873293227E-5 0.0 6.151882476037667E-4 1.2003673123975936E-4 24-25 9.002754842981953E-5 2.0006121873293227E-5 0.0 0.0018955800474945335 1.550474445180225E-4 26-27 1.0003060936646614E-4 2.0006121873293227E-5 0.0 0.003426048370801465 1.6004897498634582E-4 28-29 1.0003060936646614E-4 2.5007652341616536E-5 0.0 0.0069321212290961035 1.6004897498634582E-4 30-31 1.3003979217640597E-4 3.000918280993984E-5 0.0 0.01778544234535768 1.6004897498634582E-4 32-33 1.6004897498634582E-4 4.501377421490976E-5 0.0 0.04380840537204385 1.7005203592299244E-4 34-35 1.6505050545466914E-4 6.501989608820298E-5 0.0 0.0838206491186303 1.9005815779628567E-4 36-37 1.7005203592299244E-4 9.002754842981953E-5 0.0 0.14782023299129532 1.9505968826460897E-4 38-39 1.7005203592299244E-4 1.1003367030311275E-4 0.0 0.23801783345703786 2.150658101379022E-4 40-41 1.7005203592299244E-4 1.1003367030311275E-4 0.0 0.33908876116091524 2.250688710745488E-4 42-43 1.7005203592299244E-4 1.7005203592299244E-4 0.0 0.4406648434420933 2.300704015428721E-4 44-45 1.7505356639131573E-4 1.7005203592299244E-4 0.0 0.5635474455183286 2.3507193201119543E-4 46-47 1.8005509685963905E-4 1.8005509685963905E-4 0.0 0.7082467234973902 2.4007346247951873E-4 48-49 1.8005509685963905E-4 1.9005815779628567E-4 0.0 0.8626789797678089 2.5007652341616535E-4 50-51 1.8005509685963905E-4 1.9505968826460897E-4 0.0 1.03166068817058 2.5007652341616535E-4 52-53 1.9005815779628567E-4 2.000612187329323E-4 0.0 1.1987118058125787 2.950902976310751E-4 54-55 1.9005815779628567E-4 2.050627492012556E-4 0.0 1.385328910646658 3.451056023143082E-4 56-57 1.9005815779628567E-4 2.100642796695789E-4 0.0 1.6001996610962954 3.551086632509548E-4 58-59 2.000612187329323E-4 2.150658101379022E-4 0.0 1.8406482383609384 3.601101937192781E-4 60-61 2.000612187329323E-4 2.300704015428721E-4 0.0 2.087863886349223 3.651117241876014E-4 62-63 2.000612187329323E-4 2.300704015428721E-4 0.0 2.3410463601862173 3.8011631559257134E-4 64-65 2.000612187329323E-4 2.300704015428721E-4 0.0 2.5985401532869057 3.8011631559257134E-4 66-67 2.000612187329323E-4 2.5007652341616535E-4 0.0 2.8880537444458003 3.9011937652921793E-4 68-69 2.100642796695789E-4 2.5007652341616535E-4 0.0 3.213948468231279 4.001224374658646E-4 70-71 2.100642796695789E-4 2.5007652341616535E-4 0.0 3.5557380558450884 4.301316202758044E-4 72-73 2.100642796695789E-4 2.5007652341616535E-4 0.0 3.8940215706006036 5.001530468323307E-4 74-75 2.100642796695789E-4 2.5007652341616535E-4 0.0 4.228113802823664 5.001530468323307E-4 76-77 2.100642796695789E-4 2.5007652341616535E-4 0.0 4.593635652509668 5.05154577300654E-4 78-79 2.100642796695789E-4 2.550780538844886E-4 0.0 4.993473002738838 5.101561077689773E-4 80-81 2.200673406062255E-4 2.6007958435281194E-4 0.0 5.405128969464656 5.20159168705624E-4 82-83 2.300704015428721E-4 2.6007958435281194E-4 0.0 5.832149637789163 5.401652905789172E-4 84-85 2.4007346247951873E-4 2.6007958435281194E-4 0.0 6.258825200511357 5.401652905789172E-4 86-87 2.4007346247951873E-4 2.6007958435281194E-4 0.0 6.700045213835434 5.401652905789172E-4 88 2.4007346247951873E-4 2.6007958435281194E-4 0.0 7.044665667694314 5.501683515155638E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13960 0.0 48.81365 1 GTATCAA 26050 0.0 42.192886 1 ATCAACG 31315 0.0 34.418034 3 CAACGCA 31715 0.0 33.945694 5 TATCAAC 32755 0.0 33.317684 2 TCAACGC 32635 0.0 33.083427 4 AACGCAG 33980 0.0 32.041916 6 ACGCAGA 38600 0.0 27.963789 7 CGCAGAG 39755 0.0 27.06876 8 TACCTGG 7985 0.0 26.793695 2 GCAGAGT 44605 0.0 24.019983 9 GTACATG 35830 0.0 23.65544 1 TACATGG 36575 0.0 22.795326 2 GTACCTG 9900 0.0 22.043362 1 ACATGGG 36340 0.0 22.040737 3 ACCTGGG 9955 0.0 21.304663 3 GAGTACT 24565 0.0 20.984346 12-13 GTGGTAT 7375 0.0 20.236023 1 AGAGTAC 41410 0.0 19.861988 10-11 CAGAGTA 43595 0.0 19.651928 10-11 >>END_MODULE