FastQCFastQC Report
Mon 6 Jun 2016
ERR1211283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3651185
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185440.5078899042365698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137170.37568624980656967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98570.26996714765206364No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69380.1900205001937727No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36770.10070703073111881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68950.054.974251
TACCTGG35600.044.5158462
GTATCAA129550.043.525151
ACCTGGG42400.036.23763
GTACCTG45650.035.6446081
ATCAACG161100.034.3871153
CAACGCA162250.034.0275465
TATCAAC165200.033.7890052
TCAACGC166050.033.3620264
AACGCAG171200.032.6324466
ACGCAGA189850.029.2048427
CGCAGAG195700.028.355848
GTACATG147800.027.905111
TACATGG147800.027.458142
GCAGAGT210250.025.905059
GTGGTAT28450.025.7870031
ACATGGG153550.025.7349683
CCTGGGG43950.025.4445974
CATGGGG104500.023.7407884
ATGGGGG68750.023.3055385
Produced by FastQC (version 0.11.5)