FastQCFastQC Report
Mon 6 Jun 2016
ERR1211283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3651185
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158230.4333661537281732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124840.3419163915276821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95760.2622710161221631No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.14329594364569312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.055.8632931
TACCTGG35100.041.5709272
GTATCAA119200.041.067021
ACCTGGG41250.034.4622153
GTACCTG43100.034.0841331
TAACGCA4500.031.2784444
ATCAACG157350.030.7110733
CAACGCA160800.030.1347375
TATCAAC161700.030.0888182
TCAACGC162450.029.8054984
AACGCAG171250.028.7337346
ATAACGC4650.028.2507253
GTACATG138100.026.5425421
TACATGG143250.025.1210122
ACGCAGA194250.024.9934657
CGCAGAG199250.024.295658
ACATGGG144050.023.971163
CCTGGGG42000.023.1237094
CATGGGG96800.022.0047764
ATGGGGG62700.021.9206395
Produced by FastQC (version 0.11.5)