FastQCFastQC Report
Mon 6 Jun 2016
ERR1211282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976371
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182040.45780436483416664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144790.3641259832143429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85060.21391364135791152No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73870.18577240403372824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76900.056.9419671
GTATCAA136200.040.29981
TACCTGG35650.035.752182
ATCAACG167900.032.100913
CAACGCA168950.031.7901545
TATCAAC171800.031.619252
TCAACGC173450.031.2633954
AACGCAG177600.030.665926
GTACCTG43250.030.0145681
GTGGTAT34550.028.9961721
ACCTGGG45000.027.880683
ACGCAGA198450.027.0418267
TGGTATC36400.026.7461762
CGCAGAG203900.026.2946628
TAACGCA3950.026.1715954
GTACATG157950.025.8469451
TACATGG161450.024.8778342
GCAGAGT222350.023.6499569
ACATGGG164650.023.6019783
CATGGGG111650.022.4322644
Produced by FastQC (version 0.11.5)