FastQCFastQC Report
Mon 6 Jun 2016
ERR1211282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976371
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161280.40559595671530646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133990.3369655396843001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83750.21061918015195263No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60460.15204818665059172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78000.055.10911
GTATCAA135950.039.4538541
TACCTGG38100.039.1590082
ACCTGGG44400.032.7614333
GTACCTG47600.032.5333981
ATCAACG167650.031.3192043
CAACGCA170600.030.8798835
TATCAAC172750.030.6351872
TCAACGC172900.030.4771524
AACGCAG179500.029.7150446
GTGGTAT33300.028.888971
GTACATG146850.027.1303981
ACGCAGA201700.026.3049267
TACATGG150700.026.1514382
CGCAGAG205550.025.7894048
TGGTATC36100.025.4729022
ACATGGG152700.024.7673853
GCAGAGT224700.023.4024359
CCTGGGG40550.022.912054
CATGGGG102600.021.9524754
Produced by FastQC (version 0.11.5)