##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211282_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3976371 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03366964501049 33.0 31.0 34.0 30.0 34.0 2 32.16104835288257 33.0 31.0 34.0 30.0 34.0 3 32.22198230497104 34.0 31.0 34.0 30.0 34.0 4 35.88705430152267 37.0 35.0 37.0 35.0 37.0 5 35.791772950763395 37.0 35.0 37.0 35.0 37.0 6 35.81174140944092 37.0 35.0 37.0 35.0 37.0 7 35.73117800124787 37.0 35.0 37.0 33.0 37.0 8 35.71084564292416 37.0 35.0 37.0 33.0 37.0 9 37.314794318739374 39.0 37.0 39.0 34.0 39.0 10-11 37.2588905813869 39.0 37.0 39.0 34.0 39.0 12-13 37.18405500895163 39.0 37.0 39.0 33.0 39.0 14-15 38.38895804742565 40.0 38.0 41.0 33.0 41.0 16-17 38.36763596756943 40.0 38.0 41.0 33.0 41.0 18-19 38.38426557783467 40.0 38.0 41.0 33.5 41.0 20-21 38.355221004277524 40.0 38.0 41.0 34.0 41.0 22-23 38.23202613639421 40.0 38.0 41.0 33.5 41.0 24-25 38.134600242281216 40.0 37.0 41.0 33.0 41.0 26-27 38.02471625509793 40.0 37.0 41.0 33.0 41.0 28-29 37.910056179365554 40.0 37.0 41.0 33.0 41.0 30-31 37.886215094114704 40.0 37.0 41.0 33.0 41.0 32-33 37.787145238711375 40.0 37.0 41.0 33.0 41.0 34-35 37.625826413078656 40.0 36.5 41.0 32.5 41.0 36-37 37.45327573810391 40.0 36.0 41.0 32.0 41.0 38-39 37.32680061795039 40.0 36.0 41.0 31.5 41.0 40-41 37.20957211990531 39.5 36.0 41.0 31.0 41.0 42-43 37.08994319192047 39.0 36.0 41.0 31.0 41.0 44-45 36.920900112187724 39.0 35.5 41.0 31.0 41.0 46-47 36.66596049513488 39.0 35.0 41.0 30.0 41.0 48-49 35.11155938417215 39.0 35.0 40.0 23.0 41.0 50-51 35.83013519110767 38.5 34.5 40.0 27.5 41.0 52-53 35.92454363539016 38.0 35.0 40.0 28.0 41.0 54-55 35.75101556167672 38.0 35.0 40.0 28.0 41.0 56-57 35.53953542061342 38.0 34.0 40.0 27.5 41.0 58-59 35.30704064082552 38.0 34.0 40.0 27.0 41.0 60-61 35.05587305611071 37.5 34.0 40.0 26.5 41.0 62-63 34.73860487363981 37.0 34.0 40.0 26.0 41.0 64-65 34.40979664623849 36.0 33.0 39.0 26.0 41.0 66-67 34.065627930592996 36.0 33.0 39.0 26.0 40.0 68-69 33.70848859424837 35.5 32.5 39.0 25.5 40.0 70-71 33.34258335049722 35.0 32.0 38.0 25.0 40.0 72-73 32.95435310236394 35.0 32.0 37.0 24.0 39.5 74-75 32.58675523486113 35.0 31.0 37.0 24.0 39.0 76-77 31.66865755232598 34.0 30.5 36.0 23.0 38.0 78-79 32.10997225862476 34.0 31.0 36.0 24.0 38.0 80-81 31.98322968354814 34.0 31.0 35.5 24.0 37.0 82-83 31.711383570597413 34.0 31.0 35.0 23.5 37.0 84-85 31.422155025273042 34.0 31.0 35.0 23.0 36.5 86-87 31.1540326594274 34.0 31.0 35.0 21.5 36.0 88-89 30.919457842339156 34.0 30.0 35.0 20.0 36.0 90-91 30.690707934445754 34.0 30.0 35.0 20.0 35.0 92-93 30.49125873818112 34.0 30.0 35.0 20.0 35.0 94-95 30.288387954745673 34.0 29.0 35.0 19.0 35.0 96-97 30.0912685209705 34.0 29.0 35.0 19.0 35.0 98-99 29.85243316078907 33.5 29.0 35.0 18.0 35.0 100 27.45638296828943 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3127047235327858 1101 2 -0.24039692578041283 1101 3 -0.3349126215013918 1101 4 -0.22353490457749814 1101 5 -0.2310272937484612 1101 6 -0.2924395447422654 1101 7 -0.3309514732040668 1101 8 -0.23351329839363189 1101 9 -0.40169370120644743 1101 10-11 -0.3745209022345861 1101 12-13 -0.5048024954726955 1101 14-15 -0.589899027661609 1101 16-17 -0.5347680626375038 1101 18-19 -0.5345062256320503 1101 20-21 -0.6255388528729355 1101 22-23 -0.7247361290962786 1101 24-25 -0.7369859067648079 1101 26-27 -0.7707677641934652 1101 28-29 -0.7015448538525959 1101 30-31 -0.5933336767421551 1101 32-33 -0.5881843542027596 1101 34-35 -0.7089729849850812 1101 36-37 -0.7029143828300235 1101 38-39 -0.6923155737625919 1101 40-41 -0.7019779878782586 1101 42-43 -0.817673198874111 1101 44-45 -0.7076661427532471 1101 46-47 -0.7604233026415201 1101 48-49 0.4078738475857264 1101 50-51 -0.4988704616539792 1101 52-53 -0.8381409400340516 1101 54-55 -0.6924331638749806 1101 56-57 -0.8249750545407082 1101 58-59 -0.9998748196884435 1101 60-61 -0.8598740071728983 1101 62-63 -0.8246087132278674 1101 64-65 -0.9499877389789546 1101 66-67 -0.8842993870662781 1101 68-69 -1.0457957313435458 1101 70-71 -0.9052176413417783 1101 72-73 -0.9829707736327293 1101 74-75 -1.0502405276844158 1101 76-77 -1.0744407028216365 1101 78-79 -0.9054370113975381 1101 80-81 -0.6797009734920607 1101 82-83 -0.9635150756076172 1101 84-85 -0.8087688352680935 1101 86-87 -0.7981044001955588 1101 88-89 -0.8668477331459279 1101 90-91 -0.9661961500523653 1101 92-93 -0.8895942815005213 1101 94-95 -0.9270068043322581 1101 96-97 -1.117544106819416 1101 98-99 -1.1742014713003393 1101 100 -1.045709232200693 1102 1 -0.34346726721333454 1102 2 -0.3082558048295212 1102 3 -0.3978291952307238 1102 4 -0.2556463672845055 1102 5 -0.22615296545049546 1102 6 -0.2797561926580556 1102 7 -0.3128273448745631 1102 8 -0.2743209177027026 1102 9 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-0.9262704616083965 1102 74-75 -1.0478066725130546 1102 76-77 -0.9127449375776635 1102 78-79 -0.6915507029665093 1102 80-81 -0.6304689422618495 1102 82-83 -0.6921918195409553 1102 84-85 -0.7044293766495144 1102 86-87 -0.7176316575923494 1102 88-89 -0.8037790281490444 1102 90-91 -0.7553630883820084 1102 92-93 -0.8487022219846772 1102 94-95 -0.9980389520711661 1102 96-97 -0.9178513515195377 1102 98-99 -1.0009010398259974 1102 100 -0.9461579459071316 1103 1 -0.3343473466058242 1103 2 -0.3488002985159042 1103 3 -0.3620501969447325 1103 4 -0.23467270658687056 1103 5 -0.29987625602279877 1103 6 -0.2927583347634837 1103 7 -0.40308830553019703 1103 8 -0.39424930878855946 1103 9 -0.5329018161431947 1103 10-11 -0.444230621771986 1103 12-13 -0.5320355626877671 1103 14-15 -0.6432690036930566 1103 16-17 -0.7427135589841285 1103 18-19 -0.7202150892533368 1103 20-21 -0.7351629227056193 1103 22-23 -0.7559142530811513 1103 24-25 -0.7359911705520545 1103 26-27 -0.6975375571324491 1103 28-29 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-1.111676554015883 1103 94-95 -1.1077658295754915 1103 96-97 -1.098220033646335 1103 98-99 -1.2745393596759023 1103 100 -1.257545806444881 1104 1 -0.3562311675772136 1104 2 -0.32587031014068657 1104 3 -0.36336102308083085 1104 4 -0.1958709272974133 1104 5 -0.2061741238234731 1104 6 -0.28362958120984416 1104 7 -0.27017596422680157 1104 8 -0.3191884940941776 1104 9 -0.44528367845058625 1104 10-11 -0.45660945399728803 1104 12-13 -0.4021666389887031 1104 14-15 -0.49043971188952895 1104 16-17 -0.6625124940996514 1104 18-19 -0.5955235026865822 1104 20-21 -0.61868096479283 1104 22-23 -0.6625911178678408 1104 24-25 -0.8080367379365043 1104 26-27 -0.8061073710128142 1104 28-29 -0.7457632849690086 1104 30-31 -0.6588175603811308 1104 32-33 -0.6082899481300288 1104 34-35 -0.6527094066521357 1104 36-37 -0.6530966809697816 1104 38-39 -0.6726446932306089 1104 40-41 -0.7050244803446901 1104 42-43 -0.7523529589460267 1104 44-45 -0.6959330954226814 1104 46-47 -0.7160220247256746 1104 48-49 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0.9251093089866202 2209 80-81 0.9773795001232202 2209 82-83 1.0025190141425426 2209 84-85 1.0252380017546123 2209 86-87 1.053304451453684 2209 88-89 1.0768097428774723 2209 90-91 1.1075422391281329 2209 92-93 1.0833714374549714 2209 94-95 0.9978448857845237 2209 96-97 1.0442797476046373 2209 98-99 1.147597910313717 2209 100 1.375409663764131 2210 1 0.523099320552955 2210 2 0.4114501874465475 2210 3 0.4185231157198146 2210 4 0.2710740762186745 2210 5 0.3760639275356752 2210 6 0.41725950436449466 2210 7 0.4688214653907963 2210 8 0.4113910543914798 2210 9 0.5376573482474427 2210 10-11 0.562833488363097 2210 12-13 0.5891175216359485 2210 14-15 0.8301961546445824 2210 16-17 0.7484431546794141 2210 18-19 0.752763737547177 2210 20-21 0.8009649407819879 2210 22-23 0.8005452336440797 2210 24-25 0.8049249609407738 2210 26-27 0.8147357629138128 2210 28-29 0.8321423989709515 2210 30-31 0.8674190724179169 2210 32-33 0.7695032209574819 2210 34-35 0.9141915245052559 2210 36-37 0.9420481455294052 2210 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0.23117072851413667 2311 9 0.47664995126027065 2311 10-11 0.3457743730204115 2311 12-13 0.45207498895970843 2311 14-15 0.4754612676955148 2311 16-17 0.45686677659462305 2311 18-19 0.568364649593839 2311 20-21 0.6706836642893208 2311 22-23 0.6754269656942782 2311 24-25 0.6238443626605275 2311 26-27 0.6478955061619729 2311 28-29 0.741598734387992 2311 30-31 0.6232767828920132 2311 32-33 0.6013108480524423 2311 34-35 0.7586463693455343 2311 36-37 0.7569266868137277 2311 38-39 0.7878661546719385 2311 40-41 0.7783873092601752 2311 42-43 0.7909416141091228 2311 44-45 0.8193486390944855 2311 46-47 0.9003138413324976 2311 48-49 -5.765261500378237 2311 50-51 -0.9824362817417409 2311 52-53 0.3784757515880557 2311 54-55 0.7464890162528093 2311 56-57 0.8800992622210941 2311 58-59 0.9327037160375582 2311 60-61 0.8848130856285437 2311 62-63 0.7341571307613961 2311 64-65 0.862498250504018 2311 66-67 0.9119238327220671 2311 68-69 0.9341544359700578 2311 70-71 0.9823503003101948 2311 72-73 0.3894054448812909 2311 74-75 0.7949778481996503 2311 76-77 0.84743711245601 2311 78-79 0.7741300230725017 2311 80-81 0.8317315473061271 2311 82-83 0.8606499647851393 2311 84-85 0.8984323612247174 2311 86-87 0.9638556553277908 2311 88-89 1.0190804319509184 2311 90-91 0.937072521518143 2311 92-93 0.9680115145904225 2311 94-95 1.0410170793338764 2311 96-97 1.0326386462272445 2311 98-99 0.9921862222586384 2311 100 1.2900975282720566 2312 1 0.14526939445640252 2312 2 0.22648425981434173 2312 3 0.31214822369562967 2312 4 0.043399155400933864 2312 5 0.2071019350220027 2312 6 0.15251576696167035 2312 7 0.3005505180829928 2312 8 0.3805656392844696 2312 9 0.3050241780775593 2312 10-11 0.2635766019056618 2312 12-13 0.33674997196707324 2312 14-15 0.39654105338726 2312 16-17 0.5290075082642147 2312 18-19 0.38131086299107864 2312 20-21 0.3905438351096606 2312 22-23 0.5677321037851684 2312 24-25 0.66347400595469 2312 26-27 0.661861206814379 2312 28-29 0.7193626216410678 2312 30-31 0.8334213279126814 2312 32-33 0.8473021939875238 2312 34-35 0.5023403391707717 2312 36-37 0.7052761424580964 2312 38-39 0.8045371729672226 2312 40-41 0.5567849970317624 2312 42-43 0.6998481444373752 2312 44-45 0.8413458616202192 2312 46-47 0.9447045683902999 2312 48-49 -4.557524966895059 2312 50-51 -0.6586418365956774 2312 52-53 0.6605847873191877 2312 54-55 0.9684164708196832 2312 56-57 1.068090470039266 2312 58-59 1.1005559177014916 2312 60-61 1.0475182093401472 2312 62-63 0.8783302527380243 2312 64-65 0.970697286429079 2312 66-67 1.034632097174324 2312 68-69 1.0279285325222816 2312 70-71 1.0157901066437987 2312 72-73 1.0955784665812587 2312 74-75 0.6706266310916931 2312 76-77 0.867819526011143 2312 78-79 0.6815079255969678 2312 80-81 0.7910801699920214 2312 82-83 0.8819364055360523 2312 84-85 0.913903039525362 2312 86-87 0.9977348579013068 2312 88-89 1.0088657405688934 2312 90-91 0.7667287286670152 2312 92-93 1.0313030912091214 2312 94-95 1.175271420674683 2312 96-97 1.2083284621391641 2312 98-99 1.250446866838896 2312 100 0.7569401520959929 2313 1 0.0626995949118303 2313 2 0.2172338249879857 2313 3 0.3088961675812669 2313 4 0.09446450414230867 2313 5 0.09362270511446269 2313 6 0.2183224544845217 2313 7 0.1443328398525523 2313 8 0.2576380535912719 2313 9 0.42643969020529937 2313 10-11 0.18799057787268225 2313 12-13 -0.014438569512172705 2313 14-15 0.07415673460876349 2313 16-17 0.12443675297229362 2313 18-19 0.4386172032332709 2313 20-21 0.4020245566795637 2313 22-23 0.46304628154256733 2313 24-25 0.2667979365914235 2313 26-27 0.4224301957149379 2313 28-29 0.4087164377622514 2313 30-31 0.5093861684054204 2313 32-33 0.40145031161022615 2313 34-35 0.3897872071634936 2313 36-37 -0.4116916136444644 2313 38-39 0.19436789540774413 2313 40-41 0.4265487010751201 2313 42-43 0.5570646173427107 2313 44-45 0.5918129009122666 2313 46-47 0.40564158773548087 2313 48-49 -5.226779938619252 2313 50-51 -1.3320931486201033 2313 52-53 -0.008871346005633995 2313 54-55 0.2406229412679366 2313 56-57 0.34940493219922075 2313 58-59 0.38243352593407565 2313 60-61 0.7010112337387895 2313 62-63 0.7061223312563456 2313 64-65 0.7800657386407366 2313 66-67 0.8197344820928549 2313 68-69 0.7847753788462413 2313 70-71 0.7989684212394579 2313 72-73 0.6325112334311456 2313 74-75 0.7076560367677445 2313 76-77 0.6234783145336671 2313 78-79 0.5697895731196638 2313 80-81 -0.3011954954035403 2313 82-83 0.15393866721348815 2313 84-85 0.5653359350335485 2313 86-87 0.6033969354329187 2313 88-89 0.6585165251910361 2313 90-91 0.7439342027389841 2313 92-93 0.7242835719074776 2313 94-95 0.6442299946377226 2313 96-97 0.690814198480588 2313 98-99 0.896793261407776 2313 100 0.9074313752784775 2314 1 0.07743290683027837 2314 2 0.16251283324065469 2314 3 0.23544375688598507 2314 4 0.11321112501812536 2314 5 0.17424877338036993 2314 6 0.27374934444768684 2314 7 0.03079691108746374 2314 8 0.24209011662507152 2314 9 0.35828085517529473 2314 10-11 0.29044909313343936 2314 12-13 0.2213018569085179 2314 14-15 0.21023051286947947 2314 16-17 0.4680472504983513 2314 18-19 0.5386686915020036 2314 20-21 0.5873933951068651 2314 22-23 0.25227171687951255 2314 24-25 0.3820933232689825 2314 26-27 0.43595776341970094 2314 28-29 0.6030140954756433 2314 30-31 0.6012261370497853 2314 32-33 0.5564746148662252 2314 34-35 0.3456875710844258 2314 36-37 0.46766043392302237 2314 38-39 0.43900872011894165 2314 40-41 0.23672701968902032 2314 42-43 0.3738648626886132 2314 44-45 0.5909060728672202 2314 46-47 0.6629355388222891 2314 48-49 -6.288558755856386 2314 50-51 -1.5014535308097834 2314 52-53 -0.9116622877716765 2314 54-55 -0.5070585994937176 2314 56-57 -0.05678099663196434 2314 58-59 0.4764562931343903 2314 60-61 0.6836968988495684 2314 62-63 0.5478790197741361 2314 64-65 0.6981729532113263 2314 66-67 0.816800675252523 2314 68-69 0.8341102614543132 2314 70-71 0.5309539714765421 2314 72-73 0.7612963482321362 2314 74-75 0.7957184915750588 2314 76-77 0.7584887840352899 2314 78-79 0.5624668705605842 2314 80-81 0.5706109430755788 2314 82-83 0.5606911983344816 2314 84-85 0.5524666769008455 2314 86-87 0.5972812989942966 2314 88-89 0.4905170460223012 2314 90-91 0.7039361128942652 2314 92-93 0.7079649632936658 2314 94-95 0.7788323254237426 2314 96-97 0.854818214433358 2314 98-99 0.9067181374459707 2314 100 -0.639434515957678 2315 1 0.1555957189128243 2315 2 0.20036489322025375 2315 3 0.3246429487514746 2315 4 0.1559802488757569 2315 5 0.3115323254522693 2315 6 0.3212200156126954 2315 7 0.3749171027887499 2315 8 0.3175478273219241 2315 9 0.4267380988661884 2315 10-11 0.24844290434329963 2315 12-13 0.42118846471156246 2315 14-15 0.32598879252275736 2315 16-17 0.43183220569341785 2315 18-19 0.43400722689001725 2315 20-21 0.41976097713084926 2315 22-23 0.4974517018203599 2315 24-25 0.5150004106840385 2315 26-27 0.5356252756765514 2315 28-29 0.6486352326305891 2315 30-31 0.610767951291983 2315 32-33 0.6794183515400292 2315 34-35 0.7027734941406578 2315 36-37 0.5099973346986317 2315 38-39 0.6761409529218767 2315 40-41 0.7955280317610587 2315 42-43 0.8030754724929565 2315 44-45 0.7421868518280448 2315 46-47 0.6419817125496081 2315 48-49 -4.517826487391851 2315 50-51 -0.6775736815038798 2315 52-53 0.3923004711113691 2315 54-55 0.2624176636603863 2315 56-57 0.6412301730669441 2315 58-59 0.7476154528312122 2315 60-61 0.6121110492950592 2315 62-63 0.44874020733130493 2315 64-65 0.6194706860813497 2315 66-67 -0.11756256092489537 2315 68-69 0.6719657356484277 2315 70-71 0.6435500980913602 2315 72-73 0.8183212593664493 2315 74-75 0.8797849656910159 2315 76-77 0.8201731826493237 2315 78-79 0.8085189218627775 2315 80-81 0.5757457432213755 2315 82-83 0.6304399624895112 2315 84-85 0.6654140648619737 2315 86-87 0.6549267870960804 2315 88-89 0.5777291284240498 2315 90-91 0.690874797262861 2315 92-93 0.7891295610195606 2315 94-95 0.44197761425622417 2315 96-97 0.5040363455783705 2315 98-99 0.4920796331977897 2315 100 0.5188839385399966 2316 1 0.17965189599011566 2316 2 0.26778802666921564 2316 3 0.15897382886763722 2316 4 -0.01909515082411417 2316 5 0.1512534171062967 2316 6 0.13911010199261398 2316 7 0.22814656018216084 2316 8 0.2858096828745431 2316 9 0.43101035408864163 2316 10-11 0.40855560293255877 2316 12-13 0.34815384047031017 2316 14-15 0.4975311198489365 2316 16-17 0.40865593451191273 2316 18-19 0.17753542311000814 2316 20-21 0.44366416568207967 2316 22-23 0.614038493442294 2316 24-25 0.5845257425619579 2316 26-27 0.5586518807066412 2316 28-29 0.590538095862172 2316 30-31 0.5376757543940514 2316 32-33 0.5339228043412803 2316 34-35 0.5129853451329751 2316 36-37 0.39127423341754053 2316 38-39 0.568346120388675 2316 40-41 0.1916077935113023 2316 42-43 0.07517131198785165 2316 44-45 0.3375616238812853 2316 46-47 0.32564060370660997 2316 48-49 -4.789902597442733 2316 50-51 -0.9595093479512968 2316 52-53 0.2939460751363754 2316 54-55 0.5802151786697891 2316 56-57 0.5088757781842403 2316 58-59 0.39038531020940326 2316 60-61 0.46184329775625343 2316 62-63 0.37771830496324554 2316 64-65 0.4344931080141947 2316 66-67 0.5365220757047098 2316 68-69 0.26005972844916414 2316 70-71 -0.2923443643566159 2316 72-73 -0.09473854483372435 2316 74-75 0.22673272285146595 2316 76-77 0.33243818380805834 2316 78-79 0.3211623779043009 2316 80-81 0.2656291260505057 2316 82-83 0.19319763995119388 2316 84-85 0.3079031133138521 2316 86-87 0.2546206006888312 2316 88-89 0.11121670793120586 2316 90-91 0.1994466376527022 2316 92-93 0.3584122002557315 2316 94-95 0.5043768393510177 2316 96-97 0.6002409603476657 2316 98-99 0.6280336617272475 2316 100 -0.11230230031161881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 2.0 11 7.0 12 34.0 13 112.0 14 468.0 15 1467.0 16 3429.0 17 6996.0 18 11904.0 19 16720.0 20 21746.0 21 28728.0 22 34121.0 23 36395.0 24 37198.0 25 39876.0 26 46053.0 27 56159.0 28 68076.0 29 84656.0 30 102927.0 31 127463.0 32 159612.0 33 205846.0 34 284002.0 35 398259.0 36 595647.0 37 861172.0 38 668063.0 39 79224.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.174022111111 19.4127786403488 13.870938690821784 23.54226055771841 2 15.987792640878808 20.83879696903395 39.74433454300366 23.42907584708359 3 18.599264102131418 26.387327930243863 30.487477639765142 24.525930327859577 4 12.770346073158828 17.183068122934202 37.88972848425088 32.15685731965609 5 13.052899160488066 37.109837695700435 34.50881434719912 15.328448796612376 6 29.97148152712147 36.76976740092094 18.675425073622424 14.583325998335164 7 26.836486544260218 32.56140650895163 22.594175089340276 18.007931857447872 8 25.74910341983089 34.95098359748706 20.734416103736713 18.565496878945332 9 26.489250904613144 16.32783024384502 20.939716182734998 36.24320266880684 10-11 24.90337501208716 26.069061481747035 28.380038711609824 20.64752479455598 12-13 25.386684726707752 24.07521088350519 28.513522145597257 22.024582244189794 14-15 22.86712289562882 25.421943050513963 26.644089100450007 25.06684495340721 16-17 22.032187769955147 27.944713185819676 27.416767366092813 22.60633167813236 18-19 21.707927079220497 26.980587822561787 29.64922781330711 21.66225728491061 20-21 22.64463450062972 26.323946878156146 29.5088256418923 21.522592979321832 22-23 22.51771154580784 26.078442073041153 29.413451142891706 21.990395238259303 24-25 22.06729027571507 26.26207146996777 29.54362012886378 22.127018125453382 26-27 22.008237165171835 26.324500146867695 29.4430872595293 22.224175428431174 28-29 21.94314184159855 26.543535646595036 29.549810145451833 21.963512366354582 30-31 22.48310564628094 26.293953266824342 29.482871380591963 21.74006970630275 32-33 22.332938921597243 26.529079052159304 29.176638568236353 21.961343458007097 34-35 22.469580738131494 26.57586038455521 29.24019633736341 21.714362539949885 36-37 22.42436476463937 26.466917956128732 29.09981021231318 22.008907066918713 38-39 22.384924845708923 26.636580886306362 29.049924645106305 21.928569622878406 40-41 22.40931557658747 26.347732332975955 29.26074253699343 21.98220955344314 42-43 22.20740197690364 26.540783304526283 28.979337515333548 22.272477203236534 44-45 22.109804746123178 26.837190515198305 28.87364521606246 22.179359522616053 46-47 22.163707411863403 26.54250912965602 28.57685768803243 22.716925770448146 48-49 22.336187666860774 26.664070082320634 28.50618120722079 22.493561043597797 50-51 22.21124991526576 26.915682418452963 28.343091238435424 22.529976427845856 52-53 22.211958221646825 27.05883728982258 28.088292092802607 22.64091239572799 54-55 22.123686811652888 26.886690425828906 28.364239526560546 22.625383235957656 56-57 22.324196247196035 26.91857305221466 27.987946380179206 22.769284320410094 58-59 22.13314641246233 26.900018460699588 28.275139373251708 22.69169575358638 60-61 22.41087680536597 27.137513698806703 28.040946464151723 22.4106630316756 62-63 22.35276630596963 27.059860768906034 28.03082404742494 22.556548877699395 64-65 22.10740423622402 26.78446560231169 28.118120562951752 22.990009598512533 66-67 22.096572969313858 26.85585496407974 28.010130649200992 23.037441417405415 68-69 21.96531093948847 26.876940596826522 28.068724578700404 23.0890238849846 70-71 21.998008235210367 26.64952611946212 28.134809623961953 23.217656021365563 72-73 22.03523915698897 27.00844480040569 27.807307524354393 23.14900851825095 74-75 22.062362785849977 27.120797244864363 27.664761985244944 23.152077984040716 76-77 21.92793472808041 27.017277158286568 27.7015925228605 23.353195590772522 78-79 21.824864120405273 27.04576427013974 27.737847608869988 23.391524000585004 80-81 21.837268988030196 27.21743198059481 27.502624957403388 23.442674073971613 82-83 22.100642310496774 27.0676514907806 27.70403865583883 23.12766754288379 84-85 22.071698154973383 27.250490762792783 27.342103171369036 23.3357079108648 86-87 21.81472746463072 27.292752278716254 27.539615302187105 23.352904954465924 88-89 21.774348950471936 27.04978717623619 27.5723500171064 23.603513856185472 90-91 22.004101296102636 27.030572326375633 27.533808023875807 23.431518353645924 92-93 21.927468023383035 27.15353907090617 27.425441550713124 23.49355135499767 94-95 21.602797796902188 26.992311742439913 27.53837055316497 23.86651990749293 96-97 21.632711520924616 26.99729592835015 27.519549945098692 23.85044260562654 98-99 21.55751888299354 27.046367670267635 27.35476359268045 24.041349854058378 100 21.487974435628303 27.06085953709106 27.43181161219621 24.01935441508443 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2239.0 1 1702.5 2 3021.5 3 4238.0 4 2563.0 5 1548.0 6 2008.0 7 3575.0 8 5538.5 9 6260.0 10 5242.0 11 4078.5 12 3933.0 13 4293.5 14 4762.5 15 5127.0 16 5573.5 17 6079.0 18 6590.5 19 6974.0 20 7502.0 21 8415.5 22 9442.0 23 10977.0 24 13067.5 25 15751.5 26 19523.5 27 23837.0 28 28543.5 29 34743.5 30 42194.5 31 49324.5 32 57966.0 33 67302.0 34 76347.0 35 84833.0 36 94602.5 37 105995.0 38 112531.5 39 115354.0 40 116826.0 41 118222.5 42 121173.5 43 125936.5 44 131672.0 45 138647.0 46 145607.0 47 150558.5 48 154849.5 49 157660.5 50 157313.5 51 154476.5 52 149798.0 53 144415.0 54 138928.5 55 129281.0 56 116531.0 57 103593.5 58 91342.5 59 79850.5 60 65680.0 61 51901.5 62 41710.0 63 32383.0 64 23686.0 65 17345.0 66 13007.0 67 9853.0 68 7665.0 69 5853.0 70 4446.5 71 3383.5 72 2737.5 73 2235.5 74 1677.0 75 1245.5 76 864.5 77 559.0 78 364.5 79 241.0 80 151.5 81 80.5 82 44.0 83 30.0 84 24.0 85 15.0 86 8.0 87 5.0 88 4.0 89 4.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02954955661833365 2 0.0020370332647532134 3 1.0059423529645497E-4 4 0.022910337088767625 5 3.269312647134787E-4 6 2.5148558824113744E-5 7 2.5148558824113744E-5 8 0.003797432382441176 9 0.009103778294329175 10-11 0.0011191108676730619 12-13 1.2574279412056873E-4 14-15 1.2574279412056873E-4 16-17 4.023769411858199E-4 18-19 2.5148558824113744E-5 20-21 7.544567647234124E-5 22-23 2.5148558824113746E-4 24-25 3.772283823617062E-5 26-27 7.544567647234124E-5 28-29 9.430709559042655E-4 30-31 0.0029298071030092513 32-33 0.010248037720826352 34-35 0.019238647500447015 36-37 0.02203013752992364 38-39 0.021363700721084625 40-41 0.00482852329422984 42-43 0.005117731720707147 44-45 0.00925466964727386 46-47 0.011430019985559698 48-49 1.210488156160479 50-51 0.01774230825041225 52-53 0.019402113132803756 54-55 0.009078629735505063 56-57 0.007645161882530579 58-59 0.008978035500208608 60-61 0.005117731720707147 62-63 0.008034964544304342 64-65 0.01898716191220588 66-67 0.027852028897705973 68-69 0.027965197412414485 70-71 0.025198855941761973 72-73 0.022809742853471166 74-75 0.009506155235514995 76-77 0.014397549926805121 78-79 0.008361895809017821 80-81 0.0029172328235971944 82-83 0.014284381412096606 84-85 0.023312714029953444 86-87 0.022306771676988895 88-89 0.03146084708896629 90-91 0.020081124221054826 92-93 0.012498833735584533 94-95 0.0060985255148475834 96-97 0.003646541029496493 98-99 0.016032206250372515 100 0.03681749011850252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3976371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.765934842484 #Duplication Level Percentage of deduplicated Percentage of total 1 78.23769605900284 50.67137525183447 2 13.89440238316613 17.997679188467846 3 3.870089335973436 7.519498613047433 4 1.4566260877480828 3.7735900115581895 5 0.725855151646392 2.3505343728305794 6 0.4479263029365522 1.7406219450134097 7 0.2851755791613614 1.292876408504266 8 0.1995851229370689 1.0341053654137093 9 0.15514243037196823 0.9043150083097955 >10 0.6781373415498526 7.553631189174764 >50 0.03330566509867652 1.4674555240752907 >100 0.014970596335039948 1.7125813961639011 >500 6.346340443655598E-4 0.2807899344238635 >1k 2.71986017039514E-4 0.41067436010529823 >5k 9.0662005679838E-5 0.4233868277865675 >10k+ 9.0662005679838E-5 0.8668846032906163 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16128 0.40559595671530646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13399 0.3369655396843001 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8375 0.21061918015195263 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6046 0.15204818665059172 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.5148558824113747E-5 9 0.0 0.0 0.0 0.0 2.5148558824113747E-5 10-11 0.0 0.0 0.0 0.0 2.5148558824113747E-5 12-13 0.0 0.0 0.0 0.0 2.5148558824113747E-5 14-15 0.0 0.0 0.0 0.0 3.772283823617062E-5 16-17 0.0 0.0 0.0 0.0 5.0297117648227494E-5 18-19 0.0 0.0 0.0 6.287139706028437E-5 7.544567647234124E-5 20-21 0.0 0.0 0.0 1.6346563235673935E-4 7.544567647234124E-5 22-23 0.0 0.0 0.0 5.029711764822749E-4 7.544567647234124E-5 24-25 0.0 0.0 0.0 0.0012448536617936305 7.544567647234124E-5 26-27 0.0 0.0 0.0 0.002690895794180171 7.544567647234124E-5 28-29 0.0 0.0 0.0 0.006739813764862484 7.544567647234124E-5 30-31 0.0 0.0 0.0 0.018358447941603035 7.544567647234124E-5 32-33 0.0 0.0 0.0 0.046348793912841635 7.544567647234124E-5 34-35 0.0 0.0 0.0 0.08652361663436334 7.544567647234124E-5 36-37 0.0 0.0 0.0 0.15092907578291864 7.544567647234124E-5 38-39 0.0 0.0 0.0 0.24463260596156647 7.544567647234124E-5 40-41 0.0 0.0 0.0 0.3512121982581605 7.544567647234124E-5 42-43 0.0 0.0 0.0 0.4551386175988106 7.544567647234124E-5 44-45 0.0 0.0 0.0 0.5791713097193396 8.801995588439811E-5 46-47 0.0 0.0 0.0 0.7253598821639128 1.1316851470851187E-4 48-49 0.0 0.0 0.0 0.8832551087411109 1.7603991176879622E-4 50-51 0.0 0.0 0.0 1.055070062627456 1.7603991176879622E-4 52-53 0.0 0.0 0.0 1.2259922426755452 1.8861419118085308E-4 54-55 0.0 0.0 0.0 1.4184290147976637 2.0118847059290998E-4 56-57 0.0 0.0 0.0 1.640956037552834 2.0118847059290998E-4 58-59 0.0 0.0 0.0 1.885903000499702 2.0118847059290998E-4 60-61 0.0 0.0 0.0 2.132660659681906 2.0118847059290998E-4 62-63 0.0 0.0 0.0 2.389942990732002 2.0118847059290998E-4 64-65 0.0 0.0 0.0 2.6525945390910457 2.0118847059290998E-4 66-67 0.0 0.0 0.0 2.949221790421467 2.0118847059290998E-4 68-69 0.0 0.0 0.0 3.275285429855514 2.0118847059290998E-4 70-71 0.0 0.0 0.0 3.614250279966331 2.263370294170237E-4 72-73 0.0 0.0 0.0 3.9488518551211644 2.263370294170237E-4 74-75 0.0 0.0 0.0 4.283679767305415 2.263370294170237E-4 76-77 0.0 0.0 0.0 4.645001686210869 2.263370294170237E-4 78-79 0.0 0.0 0.0 5.052446061999747 2.263370294170237E-4 80-81 0.0 0.0 0.0 5.469145107435901 2.389113088290806E-4 82-83 0.0 0.0 0.0 5.891427132931007 3.01782705889365E-4 84-85 0.0 0.0 0.0 6.318877187264468 3.01782705889365E-4 86-87 0.0 0.0 0.0 6.769300450083757 3.01782705889365E-4 88 0.0 0.0 0.0 7.116790661635949 3.01782705889365E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7800 0.0 55.1091 1 GTATCAA 13595 0.0 39.453854 1 TACCTGG 3810 0.0 39.159008 2 ACCTGGG 4440 0.0 32.761433 3 GTACCTG 4760 0.0 32.533398 1 ATCAACG 16765 0.0 31.319204 3 CAACGCA 17060 0.0 30.879883 5 TATCAAC 17275 0.0 30.635187 2 TCAACGC 17290 0.0 30.477152 4 AACGCAG 17950 0.0 29.715044 6 GTGGTAT 3330 0.0 28.88897 1 GTACATG 14685 0.0 27.130398 1 ACGCAGA 20170 0.0 26.304926 7 TACATGG 15070 0.0 26.151438 2 CGCAGAG 20555 0.0 25.789404 8 TGGTATC 3610 0.0 25.472902 2 ACATGGG 15270 0.0 24.767385 3 GCAGAGT 22470 0.0 23.402435 9 CCTGGGG 4055 0.0 22.91205 4 CATGGGG 10260 0.0 21.952475 4 >>END_MODULE