FastQCFastQC Report
Mon 6 Jun 2016
ERR1211281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5510349
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221930.4027512594937272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172400.3128658457023321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99680.1808959831763832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99200.054.0140881
GTATCAA181550.042.754581
CAACGCA223350.033.7659535
ATCAACG224250.033.65173
TATCAAC230350.033.1035042
TCAACGC231750.032.5832184
AACGCAG237250.031.9860216
ACGCAGA267250.028.1314547
GTGGTAT40950.027.8007451
CGCAGAG273300.027.3876198
GTACATG239750.026.50891
TACATGG243450.025.5640242
GTACTAG16850.025.1268221
GCAGAGT296250.024.6802239
ACATGGG247350.024.4109753
TGGTATC49700.022.5268142
TACCTGG43150.022.2396812
GAGTACT166700.022.1847612-13
CAGAGTA289750.021.37516610-11
CATGGGG175850.021.2967724
Produced by FastQC (version 0.11.5)