##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211281_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5510349 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34933413473448 31.0 31.0 34.0 28.0 34.0 2 31.36398075693572 31.0 31.0 34.0 28.0 34.0 3 31.40913887668458 31.0 31.0 34.0 28.0 34.0 4 35.02152676717936 37.0 35.0 37.0 32.0 37.0 5 35.06395348098641 37.0 35.0 37.0 32.0 37.0 6 35.12892087234402 37.0 35.0 37.0 32.0 37.0 7 35.06041740731848 37.0 35.0 37.0 32.0 37.0 8 35.06406726688273 37.0 35.0 37.0 32.0 37.0 9 36.612061232419215 39.0 37.0 39.0 32.0 39.0 10-11 36.54222055626603 39.0 37.0 39.0 32.0 39.0 12-13 36.450598773326334 39.0 37.0 39.0 32.0 39.0 14-15 37.564617413524985 40.0 37.0 41.0 32.0 41.0 16-17 37.55942336864689 40.0 37.0 41.0 32.0 41.0 18-19 37.55752122052523 40.0 37.0 41.0 32.0 41.0 20-21 37.525363003323385 40.0 37.0 41.0 31.5 41.0 22-23 37.39860614999159 40.0 37.0 41.0 31.0 41.0 24-25 37.27745184560905 40.0 37.0 41.0 31.0 41.0 26-27 37.12880155140809 40.0 36.0 41.0 30.5 41.0 28-29 36.98795511863223 39.5 36.0 41.0 30.0 41.0 30-31 36.79384581629948 39.0 36.0 41.0 30.0 41.0 32-33 36.65715810377891 39.0 35.5 41.0 30.0 41.0 34-35 36.50231010776268 39.0 35.0 41.0 30.0 41.0 36-37 36.39739216154912 39.0 35.0 41.0 29.0 41.0 38-39 36.23840395590189 39.0 35.0 40.0 29.0 41.0 40-41 36.06289302183945 39.0 35.0 40.0 28.0 41.0 42-43 35.90452900533161 38.5 35.0 40.0 27.0 41.0 44-45 35.757556281825345 38.0 35.0 40.0 27.0 41.0 46-47 35.57235766736372 38.0 34.0 40.0 26.0 41.0 48-49 35.317914618475164 38.0 34.0 40.0 25.5 41.0 50-51 34.63953889309008 37.5 33.5 39.5 24.0 40.5 52-53 34.88192689791518 38.0 33.5 40.0 25.5 40.5 54-55 35.22930698218933 38.0 34.0 40.0 26.0 41.0 56-57 35.11729003008703 38.0 34.0 40.0 26.0 41.0 58-59 34.882638468089766 38.0 34.0 40.0 25.0 41.0 60-61 34.60353953987307 37.0 33.0 40.0 25.0 41.0 62-63 34.32263156108624 37.0 33.0 40.0 24.5 41.0 64-65 34.0349221074745 36.0 33.0 39.5 24.0 41.0 66-67 33.796870851555866 36.0 33.0 39.0 23.5 41.0 68-69 33.45107224605919 35.0 32.0 39.0 23.0 41.0 70-71 33.105035906074185 35.0 32.0 38.5 23.0 40.0 72-73 32.73774437880432 35.0 31.5 37.5 22.0 40.0 74-75 32.36840951453347 35.0 31.0 37.0 21.5 39.0 76-77 32.01740833475339 35.0 31.0 36.0 20.0 39.0 78-79 31.661403297685865 35.0 31.0 36.0 20.0 38.5 80-81 31.335471854867997 34.5 30.0 35.5 20.0 37.0 82-83 31.034830461736632 34.0 30.0 35.0 19.0 37.0 84-85 30.743405816945533 34.0 30.0 35.0 18.0 36.5 86-87 30.49624016555031 34.0 29.0 35.0 18.0 36.0 88-89 30.24290693747347 34.0 29.0 35.0 18.0 36.0 90-91 29.98898127868126 34.0 29.0 35.0 17.0 35.5 92-93 29.753223434668115 34.0 29.0 35.0 15.5 35.0 94-95 29.531246931909394 34.0 28.5 35.0 15.0 35.0 96-97 29.33158126644973 34.0 27.0 35.0 12.5 35.0 98-99 29.08956238525001 34.0 27.0 35.0 10.5 35.0 100 27.39736376044421 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3693300328354816 1101 2 -0.3321341033189462 1101 3 -0.4141306627525374 1101 4 -0.26608261792367216 1101 5 -0.32143855186101433 1101 6 -0.3116679587758924 1101 7 -0.3552263494858323 1101 8 -0.5021709996473689 1101 9 -0.45563926283980294 1101 10-11 -0.5754822514718967 1101 12-13 -0.4231283951737268 1101 14-15 -0.4775324317623202 1101 16-17 -0.563223537361111 1101 18-19 -0.5893945249349102 1101 20-21 -0.6263902971457043 1101 22-23 -0.5953597126350445 1101 24-25 -0.6212054352737582 1101 26-27 -0.680492665456633 1101 28-29 -0.7060550209702399 1101 30-31 -0.6818533510325011 1101 32-33 -0.7794399982328315 1101 34-35 -0.7564200189718449 1101 36-37 -0.8561253651188778 1101 38-39 -0.8765779206292947 1101 40-41 -0.8964463849885007 1101 42-43 -0.9100731462841409 1101 44-45 -0.940753714140655 1101 46-47 -0.8555205037949989 1101 48-49 -0.9409629618329163 1101 50-51 -0.8618014881782798 1101 52-53 -0.6556603974934916 1101 54-55 -0.720249969961479 1101 56-57 -0.7681112460515465 1101 58-59 -0.8470033739136724 1101 60-61 -0.8655409269395236 1101 62-63 -0.8143298227175393 1101 64-65 -0.8137951401459631 1101 66-67 -0.8263071935656328 1101 68-69 -0.8240442213297143 1101 70-71 -0.8232551243378836 1101 72-73 -0.9055540647084825 1101 74-75 -0.9332556159610448 1101 76-77 -0.9243012507368675 1101 78-79 -0.9102535129385387 1101 80-81 -0.9801314950170514 1101 82-83 -1.1545028190947413 1101 84-85 -1.0208638579841178 1101 86-87 -1.0495805982931934 1101 88-89 -1.1794680324149738 1101 90-91 -1.1315313418817503 1101 92-93 -1.2193916831616534 1101 94-95 -1.2691443275183723 1101 96-97 -1.3747192816464278 1101 98-99 -1.3656931042614815 1101 100 -1.289899445269974 1102 1 -0.43433103624708025 1102 2 -0.4626748985444529 1102 3 -0.4817893981049117 1102 4 -0.39411837427788043 1102 5 -0.4642131158132301 1102 6 -0.38386080575998704 1102 7 -0.4644503196801466 1102 8 -0.3969126866004018 1102 9 -0.48128689958735293 1102 10-11 -0.45618577388287207 1102 12-13 -0.5360553904358838 1102 14-15 -0.6198255762797729 1102 16-17 -0.6547368565594311 1102 18-19 -0.6038687372568035 1102 20-21 -0.6540172723396296 1102 22-23 -0.6988114485371071 1102 24-25 -0.7978868041016796 1102 26-27 -0.7929716156263424 1102 28-29 -0.7690400395987922 1102 30-31 -0.8240943617733762 1102 32-33 -0.8774968698572252 1102 34-35 -0.824700215116998 1102 36-37 -0.8927444351744569 1102 38-39 -1.0385643265834474 1102 40-41 -1.0736213886967576 1102 42-43 -1.0569634428577075 1102 44-45 -1.0351149772177237 1102 46-47 -1.0933351605846013 1102 48-49 -1.1038644355391725 1102 50-51 -1.000182854388612 1102 52-53 -0.8878576943898935 1102 54-55 -0.7816273487013632 1102 56-57 -0.8546982505625351 1102 58-59 -0.8609658463198571 1102 60-61 -0.912089409170413 1102 62-63 -0.8682946073330626 1102 64-65 -0.8717188197871835 1102 66-67 -0.8234633942130571 1102 68-69 -0.8894406997912654 1102 70-71 -0.9621150742545552 1102 72-73 -0.9478927990138146 1102 74-75 -0.9027218009901432 1102 76-77 -0.9537280409540401 1102 78-79 -0.9158036878811693 1102 80-81 -0.9535862411539178 1102 82-83 -0.9977437513077447 1102 84-85 -1.0349593914937003 1102 86-87 -0.9931548376635781 1102 88-89 -1.087578870132777 1102 90-91 -1.0067099927730432 1102 92-93 -1.0005521941162918 1102 94-95 -1.1537862404571477 1102 96-97 -1.1117041606698876 1102 98-99 -1.1881638526320302 1102 100 -1.247701188588561 1103 1 -0.37192933124230265 1103 2 -0.3843189692114066 1103 3 -0.3297849950003702 1103 4 -0.2771191315607382 1103 5 -0.2168012977272724 1103 6 -0.383533927970106 1103 7 -0.31531368604208865 1103 8 -0.3642646889265677 1103 9 -0.5324400784179488 1103 10-11 -0.4734336897573783 1103 12-13 -0.5491379310719324 1103 14-15 -0.5838101462421648 1103 16-17 -0.6415419217272955 1103 18-19 -0.614267796564512 1103 20-21 -0.7845448780157369 1103 22-23 -0.7289705578191032 1103 24-25 -0.7248462335513537 1103 26-27 -0.7668412830928943 1103 28-29 -0.814463910304724 1103 30-31 -0.7669989089853289 1103 32-33 -0.8182465159792613 1103 34-35 -0.792973098958754 1103 36-37 -0.8293016260065471 1103 38-39 -0.8094662121218974 1103 40-41 -0.8777606424695961 1103 42-43 -0.735120465617733 1103 44-45 -0.8458422723120052 1103 46-47 -0.8506386265116674 1103 48-49 -0.8059973690356728 1103 50-51 -0.7556236281591495 1103 52-53 -0.7098495719720859 1103 54-55 -0.6919676946230595 1103 56-57 -0.7303613523547767 1103 58-59 -0.637804973239831 1103 60-61 -0.6751944765749798 1103 62-63 -0.77755997905669 1103 64-65 -0.6436810610888131 1103 66-67 -0.6705683849307533 1103 68-69 -0.7392159912812701 1103 70-71 -0.7459482835042763 1103 72-73 -0.8755663749545874 1103 74-75 -0.8191875906572257 1103 76-77 -0.7660463489860305 1103 78-79 -0.7654306382040552 1103 80-81 -0.6863230677872707 1103 82-83 -0.8141541826394452 1103 84-85 -0.7744811689675686 1103 86-87 -0.8949464346996301 1103 88-89 -1.0053075967153227 1103 90-91 -0.9261036232893041 1103 92-93 -0.9404477402066966 1103 94-95 -1.1709790376427414 1103 96-97 -1.2021754174718566 1103 98-99 -1.2731762024136337 1103 100 -1.324341568699822 1104 1 -0.35762749602869803 1104 2 -0.344592108265271 1104 3 -0.43911089729955677 1104 4 -0.22587198788335883 1104 5 -0.14619349515730562 1104 6 -0.179652722068397 1104 7 -0.22465675925214867 1104 8 -0.1647534043565031 1104 9 -0.21774948615421863 1104 10-11 -0.23272268770878668 1104 12-13 -0.2932932462117819 1104 14-15 -0.4529366180657419 1104 16-17 -0.5464159098076209 1104 18-19 -0.6019498531136236 1104 20-21 -0.4857400004281942 1104 22-23 -0.3861874694102809 1104 24-25 -0.547088073554697 1104 26-27 -0.5838468295910957 1104 28-29 -0.5187268332625621 1104 30-31 -0.5229287928699193 1104 32-33 -0.7194129063168972 1104 34-35 -0.6731156715505406 1104 36-37 -0.671476998454807 1104 38-39 -0.6872558296086737 1104 40-41 -0.7259700088717267 1104 42-43 -0.748696807996005 1104 44-45 -0.8360985352448154 1104 46-47 -0.7729215821458837 1104 48-49 -0.715804795800274 1104 50-51 -0.6924273262695806 1104 52-53 -0.5751508876881672 1104 54-55 -0.554703802593373 1104 56-57 -0.5896299255831821 1104 58-59 -0.5196694044091075 1104 60-61 -0.5096941499224386 1104 62-63 -0.6228331077809983 1104 64-65 -0.5241371557610037 1104 66-67 -0.5645359841132205 1104 68-69 -0.48280705408039637 1104 70-71 -0.46711472072222193 1104 72-73 -0.6196989451928303 1104 74-75 -0.6021847814930297 1104 76-77 -0.6245354971451711 1104 78-79 -0.5796162737875825 1104 80-81 -0.6898788408286691 1104 82-83 -0.9052045267012723 1104 84-85 -0.8674619691717496 1104 86-87 -0.7835102425513369 1104 88-89 -0.6739108245605188 1104 90-91 -0.7320001029585832 1104 92-93 -0.760512307122319 1104 94-95 -0.7614725189738074 1104 96-97 -0.6341772397655632 1104 98-99 -0.7426649796620239 1104 100 -0.9131933250194102 1105 1 -0.3705644000946968 1105 2 -0.4779339280677881 1105 3 -0.4134388839035239 1105 4 -0.3453447116760415 1105 5 -0.2980008466221449 1105 6 -0.33117498755368047 1105 7 -0.42987115654711516 1105 8 -0.2949575272882896 1105 9 -0.39127670434697137 1105 10-11 -0.5469659700111364 1105 12-13 -0.5958509345341199 1105 14-15 -0.6350429675841198 1105 16-17 -0.7082771767450851 1105 18-19 -0.6403450417423997 1105 20-21 -0.7015848043260462 1105 22-23 -0.6663137371083394 1105 24-25 -0.7555920942986347 1105 26-27 -0.718836220997062 1105 28-29 -0.6687323454070153 1105 30-31 -0.7601434918101404 1105 32-33 -0.7137564106733905 1105 34-35 -0.8254030896849187 1105 36-37 -0.7927996976636322 1105 38-39 -0.8742676450882669 1105 40-41 -0.8429592032413566 1105 42-43 -0.8753797821847016 1105 44-45 -0.8454955995253997 1105 46-47 -0.7380072289937516 1105 48-49 -0.8552545703429075 1105 50-51 -0.911782772336835 1105 52-53 -0.7592363939915785 1105 54-55 -0.7056171626458791 1105 56-57 -0.7417161580583311 1105 58-59 -0.705930989619624 1105 60-61 -0.6570480464984669 1105 62-63 -0.616986294691273 1105 64-65 -0.5618787289178897 1105 66-67 -0.5189834586298261 1105 68-69 -0.6961458307832658 1105 70-71 -0.6975667264652117 1105 72-73 -0.7849283874445128 1105 74-75 -0.7394797757806515 1105 76-77 -0.7507906329944056 1105 78-79 -0.6352984876962466 1105 80-81 -0.711476530570156 1105 82-83 -0.8406218554216629 1105 84-85 -0.780733724593329 1105 86-87 -0.8499264061079295 1105 88-89 -0.8185600778248272 1105 90-91 -0.9475632088662174 1105 92-93 -0.8985847117283363 1105 94-95 -0.9946984695408325 1105 96-97 -1.105742930138053 1105 98-99 -0.9825323600549609 1105 100 -1.0145649734414341 1106 1 -0.3729917331518884 1106 2 -0.3940521192196904 1106 3 -0.37066295821020034 1106 4 -0.3071030295072106 1106 5 -0.2830656858295697 1106 6 -0.2763538866480033 1106 7 -0.36249646402086455 1106 8 -0.3288764448419741 1106 9 -0.4343338479839005 1106 10-11 -0.49288922963017967 1106 12-13 -0.4437281989605637 1106 14-15 -0.5276602539380235 1106 16-17 -0.5978964574091918 1106 18-19 -0.5369279157656663 1106 20-21 -0.5154471375121545 1106 22-23 -0.5380172106550773 1106 24-25 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0.8620427211405897 2311 24-25 0.8758614442629309 2311 26-27 1.0205912264531207 2311 28-29 1.0180248793075606 2311 30-31 0.9936673781111267 2311 32-33 1.0381713908238268 2311 34-35 1.007950111331283 2311 36-37 1.0150961361571618 2311 38-39 1.0799411121265976 2311 40-41 1.0848056270192572 2311 42-43 1.0937956515075271 2311 44-45 1.1538467350176447 2311 46-47 1.0713440152198928 2311 48-49 1.149233052788496 2311 50-51 1.1117050158112747 2311 52-53 1.0738664803777596 2311 54-55 1.0197705034637536 2311 56-57 0.958657223023117 2311 58-59 0.9415381282429394 2311 60-61 0.9190043967955148 2311 62-63 0.9541693702460066 2311 64-65 0.884095510004137 2311 66-67 0.924322004531426 2311 68-69 0.9631135459215443 2311 70-71 0.9344913089247129 2311 72-73 0.8694199566621137 2311 74-75 0.9392857145467843 2311 76-77 0.9451416436573368 2311 78-79 0.9803631369301549 2311 80-81 1.0095406557965028 2311 82-83 1.0404965699249082 2311 84-85 1.136580068217114 2311 86-87 1.0546360594879367 2311 88-89 1.1275780386942067 2311 90-91 1.1944943154645138 2311 92-93 1.1423729270057628 2311 94-95 1.1651937893285087 2311 96-97 1.2645999043809795 2311 98-99 1.260681481891055 2311 100 1.3407816909429116 2312 1 0.42991032659323025 2312 2 0.37552623254101647 2312 3 0.4119280243492831 2312 4 0.33101089757719393 2312 5 0.32611901075204486 2312 6 0.3948413532703512 2312 7 0.36455330062401714 2312 8 0.3865553986532646 2312 9 0.5467559210656816 2312 10-11 0.5759390608498904 2312 12-13 0.5872782659328735 2312 14-15 0.7072791148868234 2312 16-17 0.6792362022860914 2312 18-19 0.6767991500222905 2312 20-21 0.698443911492916 2312 22-23 0.7186380021872978 2312 24-25 0.7607989839547784 2312 26-27 0.8151647237823667 2312 28-29 0.7247545652481264 2312 30-31 0.7928262338116525 2312 32-33 0.7569555504857064 2312 34-35 0.7977060411562391 2312 36-37 0.8169814115821481 2312 38-39 0.8552472376233098 2312 40-41 0.8251481924416595 2312 42-43 0.8779551868260214 2312 44-45 0.9206941226902075 2312 46-47 0.8495907097074422 2312 48-49 0.8353644673632985 2312 50-51 0.8784071652011747 2312 52-53 0.6828579905951813 2312 54-55 0.6475780533739055 2312 56-57 0.6108919005488218 2312 58-59 0.6437348382845087 2312 60-61 0.7646210634073398 2312 62-63 0.7797567968327144 2312 64-65 0.7436326889036238 2312 66-67 0.7175092077680745 2312 68-69 0.7490804094200882 2312 70-71 0.7545646291989243 2312 72-73 0.8039869210529176 2312 74-75 0.7336338748576523 2312 76-77 0.7860463303382659 2312 78-79 0.8161913558324478 2312 80-81 0.8042668077236534 2312 82-83 0.8219159539455028 2312 84-85 0.8548347080335148 2312 86-87 0.8617436484539773 2312 88-89 0.8076969098247169 2312 90-91 0.9110990656017641 2312 92-93 1.0208835714430542 2312 94-95 0.9584014823507694 2312 96-97 0.9653100228926839 2312 98-99 1.002063748899161 2312 100 1.165822689497869 2313 1 0.4652664351932323 2313 2 0.42832977132913186 2313 3 0.43896781407053 2313 4 0.4329908642825302 2313 5 0.3189227739338776 2313 6 0.339943779318844 2313 7 0.34381981814829743 2313 8 0.32902963818074227 2313 9 0.4311527242098947 2313 10-11 0.46120614670585525 2313 12-13 0.49061434511049384 2313 14-15 0.643293483928197 2313 16-17 0.6138209301990258 2313 18-19 0.63918977111976 2313 20-21 0.6416747723600267 2313 22-23 0.5974722490562172 2313 24-25 0.6032668913101631 2313 26-27 0.6672443644863364 2313 28-29 0.6079553176422223 2313 30-31 0.6735416148637015 2313 32-33 0.6948125915359 2313 34-35 0.6920756802502339 2313 36-37 0.7228788432025084 2313 38-39 0.7705740330572084 2313 40-41 0.8258172158380361 2313 42-43 0.6581955904328609 2313 44-45 0.7888208055499604 2313 46-47 0.8280174635540476 2313 48-49 0.8417055010249825 2313 50-51 0.8146487701086897 2313 52-53 0.7251348207480603 2313 54-55 0.67751322940655 2313 56-57 0.7701535126875712 2313 58-59 0.7600740663711321 2313 60-61 0.729653548120389 2313 62-63 0.7184729133202552 2313 64-65 0.7473915193794838 2313 66-67 0.6975074994964814 2313 68-69 0.6792799902506488 2313 70-71 0.6523188899862191 2313 72-73 0.6139480234952472 2313 74-75 0.672728208931332 2313 76-77 0.7417139023739807 2313 78-79 0.664252661187259 2313 80-81 0.7185592077221195 2313 82-83 0.7356460913166494 2313 84-85 0.7114368625010812 2313 86-87 0.8134899031516589 2313 88-89 0.8775087100247561 2313 90-91 0.9550647687109155 2313 92-93 0.9906259783843261 2313 94-95 0.9691893465431285 2313 96-97 0.9403548234446504 2313 98-99 0.9521180899072021 2313 100 1.086680740270122 2314 1 0.38006716717551114 2314 2 0.2948994994859362 2314 3 0.3835430601755867 2314 4 0.31645579880954955 2314 5 0.31208296350511944 2314 6 0.3999538066336257 2314 7 0.453054074123294 2314 8 0.39288268946221194 2314 9 0.5345007592013644 2314 10-11 0.5286454694584393 2314 12-13 0.5902379697917297 2314 14-15 0.6281684145297035 2314 16-17 0.6752105908323429 2314 18-19 0.6800800375456291 2314 20-21 0.6641843828450646 2314 22-23 0.6754565598772402 2314 24-25 0.7688670588610904 2314 26-27 0.8017146631002419 2314 28-29 0.7588397864782763 2314 30-31 0.7641875313070372 2314 32-33 0.8239067371402342 2314 34-35 0.7834966075018812 2314 36-37 0.843661038108003 2314 38-39 0.8342586704972419 2314 40-41 0.9024309612156074 2314 42-43 0.7973092992464288 2314 44-45 0.8836560802861015 2314 46-47 0.8150335827258175 2314 48-49 0.7068786301614765 2314 50-51 0.8436458601983219 2314 52-53 0.8771633044062881 2314 54-55 0.8489262705319973 2314 56-57 0.787661166266659 2314 58-59 0.8060760548683845 2314 60-61 0.5956992408504718 2314 62-63 0.7259442573723973 2314 64-65 0.7960227528854631 2314 66-67 0.7727005867335279 2314 68-69 0.8348001177157158 2314 70-71 0.8412935531731591 2314 72-73 0.8945814993989814 2314 74-75 0.8740733550733069 2314 76-77 0.8786405713456418 2314 78-79 0.8856385425642408 2314 80-81 0.9196399229258994 2314 82-83 0.9623038451396404 2314 84-85 1.0228912414364686 2314 86-87 0.9963455788516846 2314 88-89 0.8264067730681326 2314 90-91 0.7842828955687331 2314 92-93 0.9638309925097381 2314 94-95 1.0089402783404218 2314 96-97 1.0219658455345808 2314 98-99 1.09184446531372 2314 100 1.0403379020486199 2315 1 0.27790558918044184 2315 2 0.32150983740324435 2315 3 0.28735639044151284 2315 4 0.18587764194361966 2315 5 0.22329939086464634 2315 6 0.2170826031672206 2315 7 0.26352011958191923 2315 8 0.25146185834010737 2315 9 0.119024673503354 2315 10-11 0.3009871080289983 2315 12-13 0.2797486137816634 2315 14-15 0.4118489661517586 2315 16-17 0.3083757105788578 2315 18-19 0.4911273218010237 2315 20-21 0.36045033023961537 2315 22-23 0.30462983846199876 2315 24-25 0.49129960858228117 2315 26-27 0.5056589681654913 2315 28-29 0.4560134293176361 2315 30-31 0.4529690488428173 2315 32-33 0.4507399195722357 2315 34-35 0.36151822139375156 2315 36-37 0.5843810422211178 2315 38-39 0.5970073272721024 2315 40-41 0.5925297427636451 2315 42-43 0.5232786986649529 2315 44-45 0.4894840594544547 2315 46-47 0.5824859965225215 2315 48-49 0.533899596876914 2315 50-51 0.3897511899053754 2315 52-53 0.06664504621716816 2315 54-55 0.23558808192306202 2315 56-57 0.2858761662361573 2315 58-59 0.3381323112896766 2315 60-61 0.44151751672946915 2315 62-63 0.3203514097757676 2315 64-65 0.3981712958525421 2315 66-67 0.30977914140381557 2315 68-69 0.30429673616077224 2315 70-71 0.25849556371910865 2315 72-73 0.3463246638883959 2315 74-75 0.09539288042690686 2315 76-77 0.3613984608417127 2315 78-79 0.4838903383481217 2315 80-81 0.531799548934611 2315 82-83 0.4746525189943789 2315 84-85 0.524309517838315 2315 86-87 0.5202769825070703 2315 88-89 0.38581030035397035 2315 90-91 0.5370901831006769 2315 92-93 0.7060156399627004 2315 94-95 0.6742725793285835 2315 96-97 0.6750335286284326 2315 98-99 0.8810970738443089 2315 100 0.9248943930277989 2316 1 0.08677671914900031 2316 2 0.048467286728762105 2316 3 0.0925415503550795 2316 4 -0.02522796079409062 2316 5 0.036019689426666446 2316 6 -0.09701625942602732 2316 7 -0.07385450692181905 2316 8 -0.035593352501692266 2316 9 -0.03899591532415059 2316 10-11 0.0027195535924775527 2316 12-13 0.058712484970847356 2316 14-15 -0.13492867316168855 2316 16-17 0.05809652476049365 2316 18-19 0.1568922557885557 2316 20-21 0.16747333172521905 2316 22-23 0.0934289992199453 2316 24-25 0.05035751912475206 2316 26-27 -0.015928621648555463 2316 28-29 0.03799489504106646 2316 30-31 -0.5052584602538985 2316 32-33 -0.3388115659213966 2316 34-35 -0.3856172750126987 2316 36-37 -0.19976831683591456 2316 38-39 -0.1020798603850821 2316 40-41 0.11926446867102669 2316 42-43 0.16640658917484785 2316 44-45 0.03852592807842825 2316 46-47 -0.139060278131673 2316 48-49 0.05238140077073439 2316 50-51 0.22103844250221982 2316 52-53 -0.16852377638311822 2316 54-55 -0.139581457457858 2316 56-57 -0.12205317344466948 2316 58-59 -0.4072855659175403 2316 60-61 -0.016001410596764742 2316 62-63 -0.07438693674675534 2316 64-65 -0.26468740884889286 2316 66-67 -0.5432336187541651 2316 68-69 -0.573146411079037 2316 70-71 -0.3958026851608025 2316 72-73 -0.6292950033451348 2316 74-75 -0.4616644736786242 2316 76-77 -0.10850012673238041 2316 78-79 -0.0033761647000325468 2316 80-81 0.024041837868551852 2316 82-83 0.12273130389690934 2316 84-85 0.15138397605668175 2316 86-87 0.2932416263470792 2316 88-89 0.36234990290831703 2316 90-91 -0.10504150502528731 2316 92-93 -0.22373661441618609 2316 94-95 0.22837904102336282 2316 96-97 0.4308078831613429 2316 98-99 0.36754779654462766 2316 100 0.14345968133806153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 156.0 1 417.0 2 246.0 3 246.0 4 207.0 5 187.0 6 254.0 7 672.0 8 1688.0 9 3040.0 10 4897.0 11 7958.0 12 12046.0 13 14743.0 14 16991.0 15 18799.0 16 21655.0 17 26310.0 18 31844.0 19 37194.0 20 44729.0 21 53564.0 22 60528.0 23 63006.0 24 63087.0 25 65059.0 26 70906.0 27 80221.0 28 93879.0 29 111839.0 30 135432.0 31 167729.0 32 211433.0 33 274644.0 34 377394.0 35 523435.0 36 758104.0 37 1053849.0 38 900049.0 39 201759.0 40 153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7345697466999 18.70388794770157 13.318517574608382 23.24302473099015 2 16.158213172767606 19.778227158016783 40.241891398672074 23.821668270543537 3 19.33478684333856 25.575931558700717 29.20309970810005 25.886181889860676 4 13.131751318250107 16.310380810061083 37.266650536620496 33.29121733506831 5 13.89631928243242 37.222148306520694 33.88624899918846 14.995283411858429 6 31.396908782222727 35.92748607110974 17.980038116873224 14.695567029794306 7 27.938348389589436 32.17456808431988 21.81363915967717 18.073444366413522 8 26.502224307182416 34.57946215773047 19.9496499058137 18.968663629273422 9 27.015257070491426 15.992729818201887 20.29727360921103 36.69473950209566 10-11 25.66648760410848 25.691032306238004 27.46566691786257 21.176813171790947 12-13 26.105256737043515 23.50946137509475 27.73882543645629 22.64645645140545 14-15 23.591581639414258 24.78350318210022 25.625618802220373 25.999296376265153 16-17 22.77644477885153 27.609897977699475 26.381478422437297 23.232178821011694 18-19 22.52756454647474 26.744093480046516 28.5537448633185 22.174597110160242 20-21 23.519500940919478 25.914680949360474 28.438209837254387 22.12760827246566 22-23 23.50995544843207 25.660105353223805 28.231277787990823 22.598661410353305 24-25 23.05572194678005 25.886097965523376 28.42907343650588 22.629106651190696 26-27 22.886201951170264 26.014035816609027 28.256922848404976 22.84283938381574 28-29 22.698475585343672 26.204182630529942 28.421290202743865 22.67605158138252 30-31 23.21856598507468 25.984106929662275 28.316139428996607 22.481187656266442 32-33 23.133999116178902 26.211123582082614 28.002368233857467 22.65250906788102 34-35 23.252450447949503 26.16328476269057 28.18595453246062 22.398310256899304 36-37 23.156490571295908 26.07059942747407 28.082564647454472 22.69034535377554 38-39 23.17070901204674 26.138551357263406 28.13325682303799 22.557482807651866 40-41 23.138612108529173 25.87051672560386 28.293066897875992 22.697804267990975 42-43 22.9348716622712 26.034384517107462 28.025119171526374 23.005624649094962 44-45 22.899243094322944 26.363063046540358 27.9303333722533 22.807360486883393 46-47 22.979985712641042 26.17552024109101 27.629433339519437 23.21506070674851 48-49 23.180429783833343 26.221304481332037 27.546980652445857 23.051285082388763 50-51 23.054127665755765 26.394454683286877 27.44003922031467 23.111378430642688 52-53 22.998935055838977 26.559247949126743 27.27851426806358 23.16330272697071 54-55 22.873102963801742 26.45884851426804 27.355341641955988 23.312706879974233 56-57 23.094489830306586 26.4239531796089 27.141195465545643 23.34036152453887 58-59 22.919652192670164 26.442591790484006 27.44151607056919 23.19623994627665 60-61 23.045292669466797 26.567364901306323 27.274290366972853 23.113052062254035 62-63 23.05245761824751 26.481183543241336 27.234085127295288 23.232273711215864 64-65 22.833150625858213 26.3250014185363 27.294127259728324 23.547720695877167 66-67 22.976546934727637 26.253673818481637 27.201862605770653 23.567916641020066 68-69 22.725822571144477 26.320560701505148 27.31427007773761 23.639346649612765 70-71 22.8321115582918 26.068635906013917 27.355210737469243 23.744041798225037 72-73 22.975468408181644 26.405260861808404 27.083868818017525 23.53540191199242 74-75 22.9730087956498 26.53262059815793 27.016006401325875 23.478364204866395 76-77 22.90938624235734 26.39769848033356 26.96478968692008 23.72812559038902 78-79 22.810965828866774 26.373295821901934 27.043132002353033 23.772606346878263 80-81 22.8166984385935 26.554333626659222 26.956125323636027 23.672842611111253 82-83 23.046160919005953 26.296945846367038 27.259330703714706 23.397562530912303 84-85 23.109234046211856 26.478744700440583 26.82013542503926 23.5918858283083 86-87 22.768719921071767 26.66800307919615 27.006028291816936 23.55724870791515 88-89 22.9283011014885 26.2321673430097 27.033655170560294 23.805876384941513 90-91 23.131816070683513 26.165884336152565 27.027027517845898 23.67527207531802 92-93 23.121636816958123 26.259068488592952 26.911330424564134 23.707964269884798 94-95 22.77812643564075 26.156074757613666 27.09057671178777 23.97522209495781 96-97 22.87724768232944 26.101963883803286 26.998258171619582 24.02253026224769 98-99 22.79234392318314 26.195513930934023 26.878182142631736 24.133960003251104 100 23.116176340987206 26.066398161835185 26.56671478917707 24.250710708000543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2182.0 1 1519.5 2 2063.0 3 2904.0 4 1941.5 5 1428.0 6 2000.0 7 3645.0 8 5861.5 9 6957.0 10 5938.5 11 4629.5 12 4617.0 13 5044.0 14 5465.5 15 6049.0 16 6582.0 17 6989.5 18 7463.0 19 7876.5 20 8282.0 21 8918.0 22 10081.0 23 11654.0 24 13483.0 25 15993.0 26 19356.0 27 23102.0 28 27938.5 29 34975.0 30 44238.5 31 54165.5 32 66258.0 33 79228.5 34 91453.5 35 103400.5 36 117499.5 37 134907.5 38 144830.5 39 147056.5 40 148135.5 41 148951.0 42 154004.5 43 163651.5 44 173855.5 45 187958.5 46 201104.5 47 211040.0 48 220525.5 49 225400.0 50 225806.5 51 226192.0 52 224491.0 53 219855.5 54 214412.5 55 201615.0 56 185325.5 57 165352.5 58 144454.0 59 127464.5 60 106967.5 61 86607.0 62 70554.5 63 55751.0 64 42063.0 65 31018.5 66 23179.5 67 17820.0 68 13742.0 69 10554.5 70 8276.0 71 6509.5 72 5020.5 73 4014.5 74 3261.5 75 2501.0 76 1824.0 77 1270.5 78 855.5 79 573.0 80 410.0 81 282.5 82 197.0 83 139.5 84 101.0 85 81.5 86 71.0 87 53.0 88 32.5 89 29.0 90 26.5 91 23.0 92 17.0 93 11.0 94 8.0 95 7.5 96 5.5 97 2.5 98 2.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02449935566694596 2 0.10848677642740959 3 0.016822890891302893 4 0.017458059371557047 5 0.019418007824912726 6 0.04631285604596006 7 0.046476185083739706 8 0.03841861921994414 9 0.03304690864408044 10-11 0.03687606719646977 12-13 0.03592331447608854 14-15 0.031268436899368805 16-17 0.03797400128376623 18-19 0.03721179910746125 20-21 0.049080375852781744 22-23 0.048753717777222455 24-25 0.06802654423521995 26-27 0.059796575498212545 28-29 0.05186604333046781 30-31 0.04951591995352745 32-33 0.0444073506051976 34-35 0.045904533451510965 36-37 0.04176686449442676 38-39 0.04706598438683285 40-41 0.055785940237179164 42-43 0.05736478760238235 44-45 0.060286562611551464 46-47 0.061393570534280135 48-49 0.06075840205402598 50-51 0.05442486492234883 52-53 0.06329000213961039 54-55 0.050405155825883265 56-57 0.054533750947535264 58-59 0.05216547989973049 60-61 0.04764670985449379 62-63 0.05545020832618769 64-65 0.05283694372171346 66-67 0.05074088773687474 68-69 0.05634851803397571 70-71 0.05679313597015362 72-73 0.059279366878577024 74-75 0.0497246181684681 76-77 0.05185696949503561 78-79 0.06180189312872923 80-81 0.061538751901195364 82-83 0.051412351558857705 84-85 0.057519042804729796 86-87 0.055667980376560545 88-89 0.06402498280961877 90-91 0.06953280091696551 92-93 0.07486821615110041 94-95 0.07829812594447284 96-97 0.07378842973466834 98-99 0.08214543216772657 100 0.12765071686022064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5510349.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.567172886825546 #Duplication Level Percentage of deduplicated Percentage of total 1 75.19725934259786 44.79288147875985 2 14.327122571685324 17.068523743966406 3 4.753987727399634 8.495448265795828 4 2.0356101800362585 4.850221740976076 5 1.0946870773165887 3.2603707195745497 6 0.6420684715543149 2.2947722190153423 7 0.4253090428728827 1.7734120100997515 8 0.28863065347820405 1.375432962893889 9 0.20775530512686657 1.113785656078253 >10 0.9695731958417821 9.807910173482725 >50 0.041365864274659825 1.6783291159757838 >100 0.015562687448690543 1.6028120526315268 >500 5.720788628871124E-4 0.24826316073045074 >1k 3.8138577236928505E-4 0.5155412133262971 >5k 3.81385772369194E-5 0.22645374408125546 >10k+ 7.62771544738388E-5 0.8958417426119728 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22193 0.4027512594937272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17240 0.3128658457023321 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9968 0.1808959831763832 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 0.0 5 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 0.0 6 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 0.0 7 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 0.0 8 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 0.0 9 1.8147670864404414E-5 0.0 0.0 1.0888602518642649E-4 3.629534172880883E-5 10-11 1.8147670864404414E-5 3.629534172880883E-5 0.0 1.0888602518642649E-4 3.629534172880883E-5 12-13 3.629534172880883E-5 4.536917716101103E-5 0.0 1.0888602518642649E-4 3.629534172880883E-5 14-15 3.629534172880883E-5 7.259068345761766E-5 0.0 1.0888602518642649E-4 3.629534172880883E-5 16-17 3.629534172880883E-5 9.073835432202208E-5 0.0 1.361075314830331E-4 5.444301259321324E-5 18-19 4.536917716101103E-5 9.073835432202208E-5 0.0 2.2684588580505516E-4 5.444301259321324E-5 20-21 5.444301259321324E-5 9.073835432202208E-5 0.0 2.9943656926267285E-4 5.444301259321324E-5 22-23 5.444301259321324E-5 9.073835432202208E-5 0.0 4.990609487711214E-4 5.444301259321324E-5 24-25 5.444301259321324E-5 1.088860251864265E-4 0.0 8.710882014914119E-4 5.444301259321324E-5 26-27 5.444301259321324E-5 1.270336960508309E-4 0.0 0.0019055054407624635 5.444301259321324E-5 28-29 5.444301259321324E-5 1.270336960508309E-4 0.0 0.004455253197211284 5.444301259321324E-5 30-31 5.444301259321324E-5 1.270336960508309E-4 0.0 0.01311169219953219 5.444301259321324E-5 32-33 5.444301259321324E-5 1.270336960508309E-4 0.0 0.034135768895944704 7.259068345761766E-5 34-35 5.444301259321324E-5 1.361075314830331E-4 0.0 0.06715545603372854 7.259068345761766E-5 36-37 5.444301259321324E-5 1.6332903777963975E-4 0.0 0.12141699191829773 7.259068345761766E-5 38-39 5.444301259321324E-5 1.8147670864404416E-4 0.0 0.20209246274600756 7.259068345761766E-5 40-41 5.444301259321324E-5 1.8147670864404416E-4 0.0 0.2930122937766737 7.259068345761766E-5 42-43 5.444301259321324E-5 2.3591972123725738E-4 0.0 0.38233512977127215 9.073835432202206E-5 44-45 5.444301259321324E-5 2.3591972123725738E-4 0.0 0.4960484354076303 1.1795986061862869E-4 46-47 5.444301259321324E-5 2.3591972123725738E-4 0.0 0.630341199804223 1.9055054407624635E-4 48-49 5.444301259321324E-5 2.3591972123725738E-4 0.0 0.7713576762560774 2.540673921016618E-4 50-51 5.444301259321324E-5 2.3591972123725738E-4 0.0 0.9207765243181512 2.540673921016618E-4 52-53 5.444301259321324E-5 2.540673921016618E-4 0.0 1.0707035071644282 2.9036273383047063E-4 54-55 5.444301259321324E-5 2.540673921016618E-4 0.0 1.234395498361356 3.175842401270773E-4 56-57 5.444301259321324E-5 2.540673921016618E-4 0.0 1.425064002298221 3.2665807555927944E-4 58-59 7.259068345761766E-5 2.540673921016618E-4 0.0 1.6434893688221925 3.2665807555927944E-4 60-61 7.259068345761766E-5 2.63141227533864E-4 0.0 1.86379301928063 3.2665807555927944E-4 62-63 7.259068345761766E-5 2.722150629660662E-4 0.0 2.090185213314075 4.173964298813015E-4 64-65 8.166451888981987E-5 2.722150629660662E-4 0.0 2.3198621357739775 4.173964298813015E-4 66-67 9.073835432202208E-5 3.448057464236839E-4 0.0 2.5808619381458415 4.173964298813015E-4 68-69 9.073835432202208E-5 3.448057464236839E-4 0.0 2.869328240370982 4.173964298813015E-4 70-71 9.073835432202208E-5 3.448057464236839E-4 0.0 3.1789002838114246 4.3554410074570594E-4 72-73 9.073835432202208E-5 3.448057464236839E-4 0.0 3.4804601305652327 5.807254676609413E-4 74-75 9.981218975422428E-5 3.448057464236839E-4 0.0 3.784088811797583 5.807254676609413E-4 76-77 1.0888602518642649E-4 3.629534172880883E-4 0.0 4.1198025751182 5.807254676609413E-4 78-79 1.0888602518642649E-4 3.629534172880883E-4 0.0 4.482910247608636 5.807254676609413E-4 80-81 1.0888602518642649E-4 3.720272527202905E-4 0.0 4.861180299106282 5.807254676609413E-4 82-83 1.0888602518642649E-4 3.811010881524927E-4 0.0 5.244486329268799 5.807254676609413E-4 84-85 1.0888602518642649E-4 3.811010881524927E-4 0.0 5.627964762304529 5.807254676609413E-4 86-87 1.0888602518642649E-4 3.811010881524927E-4 0.0 6.027222595156859 5.807254676609413E-4 88 1.0888602518642649E-4 3.811010881524927E-4 0.0 6.341213596452784 5.807254676609413E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9920 0.0 54.014088 1 GTATCAA 18155 0.0 42.75458 1 CAACGCA 22335 0.0 33.765953 5 ATCAACG 22425 0.0 33.6517 3 TATCAAC 23035 0.0 33.103504 2 TCAACGC 23175 0.0 32.583218 4 AACGCAG 23725 0.0 31.986021 6 ACGCAGA 26725 0.0 28.131454 7 GTGGTAT 4095 0.0 27.800745 1 CGCAGAG 27330 0.0 27.387619 8 GTACATG 23975 0.0 26.5089 1 TACATGG 24345 0.0 25.564024 2 GTACTAG 1685 0.0 25.126822 1 GCAGAGT 29625 0.0 24.680223 9 ACATGGG 24735 0.0 24.410975 3 TGGTATC 4970 0.0 22.526814 2 TACCTGG 4315 0.0 22.239681 2 GAGTACT 16670 0.0 22.18476 12-13 CAGAGTA 28975 0.0 21.375166 10-11 CATGGGG 17585 0.0 21.296772 4 >>END_MODULE