FastQCFastQC Report
Mon 6 Jun 2016
ERR1211281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5510349
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193470.35110298821363223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163740.2971499627337579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97040.17610499806818045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99900.051.531061
GTATCAA180400.041.0486071
TATCAAC232200.031.644222
CAACGCA229550.031.576565
ATCAACG229550.031.5632823
TCAACGC238050.030.5348224
AACGCAG242850.030.0980956
GTGGTAT43050.027.4697911
ACGCAGA274650.026.3738447
GTACATG233800.025.892451
GTACTAG19650.025.5534291
CGCAGAG283550.025.4136668
TACATGG239400.025.0675132
ACATGGG244200.023.7896023
GCAGAGT308850.023.0114069
CATGGGG169650.022.5432194
TACCTGG42550.022.1646752
TGGTATC51900.021.9686722
GAGTACT175900.021.2531512-13
ATGGGGG111250.021.2119985
Produced by FastQC (version 0.11.5)