##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211281_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5510349 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02739427212324 33.0 31.0 34.0 30.0 34.0 2 32.160566962274075 33.0 31.0 34.0 30.0 34.0 3 32.22092176012808 34.0 31.0 34.0 30.0 34.0 4 35.88315930624358 37.0 35.0 37.0 35.0 37.0 5 35.781906554376135 37.0 35.0 37.0 35.0 37.0 6 35.79912452006216 37.0 35.0 37.0 35.0 37.0 7 35.71198067490825 37.0 35.0 37.0 33.0 37.0 8 35.692937416486686 37.0 35.0 37.0 33.0 37.0 9 37.29059357220387 39.0 37.0 39.0 34.0 39.0 10-11 37.237856712886966 39.0 37.0 39.0 33.0 39.0 12-13 37.16118661449574 39.0 37.0 39.0 33.0 39.0 14-15 38.360667808881075 40.0 38.0 41.0 33.0 41.0 16-17 38.33924784074475 40.0 38.0 41.0 33.0 41.0 18-19 38.34827385706423 40.0 38.0 41.0 33.5 41.0 20-21 38.31611427878706 40.0 38.0 41.0 34.0 41.0 22-23 38.200050940512114 40.0 38.0 41.0 33.0 41.0 24-25 38.10081158198872 40.0 37.0 41.0 33.0 41.0 26-27 37.991387478361176 40.0 37.0 41.0 33.0 41.0 28-29 37.87917679987238 40.0 37.0 41.0 33.0 41.0 30-31 37.879027263064465 40.0 37.0 41.0 33.0 41.0 32-33 37.80297736132503 40.0 37.0 41.0 33.0 41.0 34-35 37.6599523006619 40.0 37.0 41.0 32.5 41.0 36-37 37.5012250585217 40.0 36.0 41.0 32.0 41.0 38-39 37.38000977796506 40.0 36.0 41.0 32.0 41.0 40-41 37.261640778106795 39.5 36.0 41.0 31.0 41.0 42-43 37.14244034270787 39.0 36.0 41.0 31.0 41.0 44-45 36.97252569664825 39.0 35.5 41.0 31.0 41.0 46-47 36.71451499714446 39.0 35.0 41.0 30.0 41.0 48-49 35.148321639881615 39.0 34.5 40.0 24.0 41.0 50-51 35.85635555933027 38.0 34.5 40.0 28.0 41.0 52-53 35.94382197933379 38.0 35.0 40.0 28.5 41.0 54-55 35.76121004313883 38.0 34.5 40.0 28.0 41.0 56-57 35.53413567815759 38.0 34.0 40.0 28.0 41.0 58-59 35.28043595786764 37.5 34.0 40.0 27.0 41.0 60-61 35.01213652710563 37.0 34.0 40.0 27.0 41.0 62-63 34.68021907505314 36.5 33.5 39.5 26.0 41.0 64-65 34.33422510987961 36.0 33.0 39.0 26.0 40.5 66-67 33.98776874205245 35.5 33.0 39.0 26.0 40.0 68-69 33.620878550523756 35.0 32.5 38.0 26.0 40.0 70-71 33.25909447840781 35.0 32.0 37.5 25.0 40.0 72-73 32.88382332952051 35.0 32.0 37.0 25.0 39.0 74-75 32.523268399152215 35.0 31.0 36.5 24.0 39.0 76-77 31.622801749943605 34.0 30.5 35.5 23.5 38.0 78-79 32.078155122298064 34.0 31.0 36.0 24.0 37.5 80-81 31.96770975849261 34.0 31.0 35.0 24.0 37.0 82-83 31.70793338135207 34.0 31.0 35.0 24.0 37.0 84-85 31.437932878661588 34.0 31.0 35.0 23.0 36.0 86-87 31.18086740059477 34.0 31.0 35.0 23.0 36.0 88-89 30.9520193730016 34.0 30.0 35.0 21.0 35.5 90-91 30.73017317051969 34.0 30.0 35.0 20.0 35.0 92-93 30.535986468370695 34.0 30.0 35.0 20.0 35.0 94-95 30.335690625040264 34.0 29.0 35.0 19.5 35.0 96-97 30.138718618367 34.0 29.0 35.0 19.0 35.0 98-99 29.912212094007113 33.0 29.0 35.0 18.5 35.0 100 27.455975655988397 31.0 24.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.27552877949794663 1101 2 -0.26221014515667207 1101 3 -0.3133998757087788 1101 4 -0.15076369543473334 1101 5 -0.2197875609770179 1101 6 -0.2723393969924288 1101 7 -0.2977479295897538 1101 8 -0.2535768024302385 1101 9 -0.34627822446966405 1101 10-11 -0.328288609552537 1101 12-13 -0.43258663371413775 1101 14-15 -0.5543155399231736 1101 16-17 -0.5405769476880593 1101 18-19 -0.5644628318561402 1101 20-21 -0.6719463200548077 1101 22-23 -0.6337383825008729 1101 24-25 -0.6402423132073807 1101 26-27 -0.6689699080818841 1101 28-29 -0.7212592936248683 1101 30-31 -0.7057347814539909 1101 32-33 -0.6712223061746982 1101 34-35 -0.7434468040789071 1101 36-37 -0.7740521569955874 1101 38-39 -0.7698243820993866 1101 40-41 -0.7132753216607028 1101 42-43 -0.7039487365264065 1101 44-45 -0.608803831911537 1101 46-47 -0.702985654476258 1101 48-49 0.45151659450457515 1101 50-51 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0.4715588285056782 2311 10-11 0.43114314043238977 2311 12-13 0.530135062022751 2311 14-15 0.4733871867082584 2311 16-17 0.4979912908412274 2311 18-19 0.6744949983755504 2311 20-21 0.7000742892856309 2311 22-23 0.7533593076743372 2311 24-25 0.7010277187326963 2311 26-27 0.7123763990922285 2311 28-29 0.807346088681328 2311 30-31 0.692301690404058 2311 32-33 0.6894557639696757 2311 34-35 0.8077230238926347 2311 36-37 0.8673280428380608 2311 38-39 0.8610391867852201 2311 40-41 0.8727680525205699 2311 42-43 0.8599423564647992 2311 44-45 0.9410496763759397 2311 46-47 0.9493011827735742 2311 48-49 -5.837263094275116 2311 50-51 -0.9729647609547882 2311 52-53 0.3811367033991928 2311 54-55 0.8073693121684187 2311 56-57 0.9172915573516462 2311 58-59 0.9727004965237498 2311 60-61 0.8017301052344337 2311 62-63 0.7023522532177893 2311 64-65 0.7939992187711269 2311 66-67 0.9158669683069007 2311 68-69 1.0033596528664646 2311 70-71 0.9016510094582557 2311 72-73 0.39149339490884927 2311 74-75 0.6923364928554534 2311 76-77 0.7962019735236865 2311 78-79 0.7286305555972987 2311 80-81 0.783998474858624 2311 82-83 0.8035887675678737 2311 84-85 0.7868231207974077 2311 86-87 0.8304091260807702 2311 88-89 0.8857326952664586 2311 90-91 1.0044208437246738 2311 92-93 0.9552020884109282 2311 94-95 0.907988202168653 2311 96-97 0.920317715080877 2311 98-99 1.0091574803665502 2311 100 1.2280086332122728 2312 1 0.10961292647047216 2312 2 0.23114994113321075 2312 3 0.21118159973153894 2312 4 -0.057575050187999466 2312 5 0.2205853637094748 2312 6 0.1569323862650407 2312 7 0.2805028867585051 2312 8 0.3344557833175159 2312 9 0.29013074993121535 2312 10-11 0.20063020478398386 2312 12-13 0.26860846288926865 2312 14-15 0.3067070331475037 2312 16-17 0.47443995918234094 2312 18-19 0.3751349529765662 2312 20-21 0.4402647949340519 2312 22-23 0.7072040090208489 2312 24-25 0.7690357470837768 2312 26-27 0.5888791078191176 2312 28-29 0.7553081460026192 2312 30-31 0.7690581484832748 2312 32-33 0.8634511000439247 2312 34-35 0.4225331143337172 2312 36-37 0.7734404913889605 2312 38-39 0.8403517930783551 2312 40-41 0.666051693166942 2312 42-43 0.7138567408005301 2312 44-45 0.8257944678066309 2312 46-47 0.8836746648651896 2312 48-49 -4.689596816311116 2312 50-51 -0.7304062865996741 2312 52-53 0.6144281628161323 2312 54-55 0.885611501181188 2312 56-57 1.0393853426060318 2312 58-59 1.1052464264221058 2312 60-61 1.0267886321112414 2312 62-63 0.9054534373223717 2312 64-65 1.0492673464097066 2312 66-67 1.04480325355307 2312 68-69 0.957001443321893 2312 70-71 1.038199496846346 2312 72-73 1.155885400070396 2312 74-75 0.890576257011567 2312 76-77 1.0004731003404004 2312 78-79 0.6882804726130374 2312 80-81 0.8956244222422747 2312 82-83 0.8788944374547754 2312 84-85 0.9222934835058538 2312 86-87 0.958245543934698 2312 88-89 1.0118831290196475 2312 90-91 0.8033902923026517 2312 92-93 1.0512431302488174 2312 94-95 1.1627126734018312 2312 96-97 1.1813752512594604 2312 98-99 1.180155473405339 2312 100 0.8907163628098758 2313 1 0.1038250084136969 2313 2 0.24326074774073447 2313 3 0.3091722483718442 2313 4 0.11610136325780473 2313 5 0.18396433485139596 2313 6 0.2672754619095059 2313 7 0.1848007809277803 2313 8 0.26812110516090115 2313 9 0.46440729244312706 2313 10-11 0.2797835598646472 2313 12-13 0.06316909478989885 2313 14-15 0.20809663340251916 2313 16-17 0.12942796330304418 2313 18-19 0.4693755582398751 2313 20-21 0.3743816346544335 2313 22-23 0.48666453278058697 2313 24-25 0.33896998334364525 2313 26-27 0.4357787537387381 2313 28-29 0.48541904415482406 2313 30-31 0.6268232779686187 2313 32-33 0.477069256513559 2313 34-35 0.47767850184899174 2313 36-37 -0.14428273703882866 2313 38-39 0.21058276271684662 2313 40-41 0.4171292794273427 2313 42-43 0.5588458877405245 2313 44-45 0.5353023123278007 2313 46-47 0.3872462978661062 2313 48-49 -5.132043515482103 2313 50-51 -1.3121396977360646 2313 52-53 0.09539624990988216 2313 54-55 0.2593240732906281 2313 56-57 0.2907798973947564 2313 58-59 0.38150065198653493 2313 60-61 0.6843569405108028 2313 62-63 0.7040821733101126 2313 64-65 0.767233989887373 2313 66-67 0.8017026030942844 2313 68-69 0.8937610899057731 2313 70-71 0.8473778896978814 2313 72-73 0.6392045325583524 2313 74-75 0.826923145760496 2313 76-77 0.9332919937150024 2313 78-79 0.8970281586149369 2313 80-81 -0.1765340298025322 2313 82-83 0.29087579321675605 2313 84-85 0.685538098913149 2313 86-87 0.8438497416261548 2313 88-89 0.8082631247770209 2313 90-91 0.7910930328895738 2313 92-93 0.9404775416003197 2313 94-95 0.8084987853870977 2313 96-97 0.8654534302270491 2313 98-99 1.0322534702099126 2313 100 1.1080637392179646 2314 1 0.12208756259424902 2314 2 0.23681261101355489 2314 3 0.2544509860768258 2314 4 0.11103768629880761 2314 5 0.29949271993304905 2314 6 0.35149496064907026 2314 7 0.1694127431515966 2314 8 0.366734978073751 2314 9 0.5516136322333693 2314 10-11 0.43919131939161815 2314 12-13 0.2677184184726755 2314 14-15 0.2966502107962441 2314 16-17 0.5629042034227183 2314 18-19 0.625136496291077 2314 20-21 0.7386578485650261 2314 22-23 0.42739258662153645 2314 24-25 0.554854882172485 2314 26-27 0.6195105031775583 2314 28-29 0.6799945096439615 2314 30-31 0.6582548000085282 2314 32-33 0.5678926546734218 2314 34-35 0.3997084509928328 2314 36-37 0.5182212630641416 2314 38-39 0.6031588641272805 2314 40-41 0.4707608610227112 2314 42-43 0.4225814661627325 2314 44-45 0.617050376336465 2314 46-47 0.7201078823105433 2314 48-49 -6.479498528583722 2314 50-51 -1.516265025130231 2314 52-53 -0.8295624766328444 2314 54-55 -0.32424166090596884 2314 56-57 0.028579290841669547 2314 58-59 0.4768571832844586 2314 60-61 0.6221418838599888 2314 62-63 0.5144930884576198 2314 64-65 0.7419363734514661 2314 66-67 0.8139982029517725 2314 68-69 0.8422719400570884 2314 70-71 0.6345644410445743 2314 72-73 0.8613357347655324 2314 74-75 0.8790198432403855 2314 76-77 0.7593585220819534 2314 78-79 0.5387813503607148 2314 80-81 0.59276796612453 2314 82-83 0.5649159732638012 2314 84-85 0.6255718021572818 2314 86-87 0.721839580925856 2314 88-89 0.6600153600082308 2314 90-91 0.8247569341468477 2314 92-93 0.9094741841464398 2314 94-95 0.973845564331608 2314 96-97 0.9637050269043463 2314 98-99 1.1388814433760217 2314 100 -0.20247792883813176 2315 1 0.0631812181447593 2315 2 0.19156632232930093 2315 3 0.256608846002635 2315 4 0.10061416625740094 2315 5 0.2346999166046757 2315 6 0.2710423321919535 2315 7 0.3360710586861302 2315 8 0.2063722542743065 2315 9 0.42137750869238744 2315 10-11 0.2451915061664991 2315 12-13 0.4053670068989703 2315 14-15 0.3081939081314644 2315 16-17 0.36401767076340263 2315 18-19 0.4423021104246203 2315 20-21 0.40379382532941577 2315 22-23 0.47025462708052146 2315 24-25 0.4790072132131016 2315 26-27 0.5321372188994999 2315 28-29 0.6136491582326755 2315 30-31 0.5912001106953326 2315 32-33 0.6468685851129905 2315 34-35 0.6455436687772931 2315 36-37 0.5448426753088498 2315 38-39 0.5895049083194408 2315 40-41 0.6242714082847414 2315 42-43 0.7095453924671133 2315 44-45 0.7370128645032779 2315 46-47 0.6220029575244723 2315 48-49 -4.682700635857572 2315 50-51 -0.8512767181675969 2315 52-53 0.3487388784000771 2315 54-55 0.2571513050298364 2315 56-57 0.5521729456660012 2315 58-59 0.739837711058577 2315 60-61 0.6470166023542205 2315 62-63 0.33653757922301253 2315 64-65 0.569217096685648 2315 66-67 -0.13452694354161565 2315 68-69 0.5975560281439556 2315 70-71 0.5574161377186755 2315 72-73 0.7547211878776281 2315 74-75 0.8538969508845895 2315 76-77 0.6986111160888271 2315 78-79 0.6445303205572017 2315 80-81 0.5894686153633266 2315 82-83 0.502920617655171 2315 84-85 0.5627034627661089 2315 86-87 0.6605694303701917 2315 88-89 0.6715131261166931 2315 90-91 0.7320226224384463 2315 92-93 0.6822322537364975 2315 94-95 0.34958177109344035 2315 96-97 0.5545627302936253 2315 98-99 0.4765588904102387 2315 100 0.49172394547543874 2316 1 0.2603535067733951 2316 2 0.282339770151232 2316 3 0.23434398343363938 2316 4 -0.03900618255475763 2316 5 0.18958513520554732 2316 6 0.13751445355447345 2316 7 0.24406827782267015 2316 8 0.33508340484689114 2316 9 0.5098184230590874 2316 10-11 0.4132061238669067 2316 12-13 0.3578029615589813 2316 14-15 0.5382252799066052 2316 16-17 0.50388970678231 2316 18-19 0.17746294651265515 2316 20-21 0.483979163376425 2316 22-23 0.6003180463581614 2316 24-25 0.6385875431105674 2316 26-27 0.6263969267823768 2316 28-29 0.6994908698538467 2316 30-31 0.6292502970417857 2316 32-33 0.583573411028496 2316 34-35 0.6064931747200859 2316 36-37 0.45794230842947314 2316 38-39 0.5594967269221343 2316 40-41 0.20276302768471055 2316 42-43 0.1401980464736141 2316 44-45 0.36704084584471275 2316 46-47 0.3707164566271288 2316 48-49 -4.849061573644459 2316 50-51 -1.00046585241504 2316 52-53 0.34522972052482004 2316 54-55 0.5585637403668073 2316 56-57 0.41974796072240395 2316 58-59 0.2886914188694405 2316 60-61 0.46863908980571267 2316 62-63 0.3724297674891446 2316 64-65 0.38333956490818366 2316 66-67 0.491819833698969 2316 68-69 0.3084046704533918 2316 70-71 -0.15518812165700524 2316 72-73 0.015121655903762132 2316 74-75 0.23309038298453544 2316 76-77 0.21223655427938581 2316 78-79 0.3162117973083767 2316 80-81 0.2990795724145734 2316 82-83 0.23512867382585512 2316 84-85 0.37155503344764185 2316 86-87 0.4705200843416826 2316 88-89 0.2483433858107027 2316 90-91 0.3533833386792473 2316 92-93 0.47549418788620557 2316 94-95 0.6793919436120142 2316 96-97 0.7591617557079147 2316 98-99 0.7027223954797748 2316 100 -0.14279789413189548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 5.0 11 20.0 12 30.0 13 138.0 14 570.0 15 1532.0 16 3699.0 17 7457.0 18 12645.0 19 18683.0 20 25820.0 21 36119.0 22 45247.0 23 48902.0 24 50462.0 25 56385.0 26 65695.0 27 79765.0 28 98922.0 29 119937.0 30 147740.0 31 181765.0 32 228223.0 33 295922.0 34 406853.0 35 568876.0 36 858173.0 37 1198038.0 38 857821.0 39 94899.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47353901623429 19.577542794935567 14.28754253898011 23.66137564985003 2 15.751163241865566 20.339578243951678 39.73012462054786 24.179133893634898 3 18.912303841647635 25.576697208014597 29.896717881860006 25.614281068477755 4 13.113853365313929 16.76075215631236 37.21294698624513 32.912447492128585 5 13.70282575445764 37.372176114763654 34.067810267142576 14.857187863636131 6 31.418547688557545 36.41527475492923 17.61299243042226 14.55318512609097 7 27.852479680949255 32.28746434434426 21.673121796707502 18.186934177998985 8 26.485429859391935 34.97287454998867 19.708468334464886 18.83322725615452 9 26.72777470850143 15.989207601577116 20.16104384112393 37.12197384879752 10-11 25.608472358696556 25.91428172953701 27.343733199063465 21.133512712702977 12-13 25.959597618551367 23.573230961009962 27.80870194389878 22.658469476539892 14-15 23.379783548903664 24.901131399418983 25.595153406030548 26.123931645646802 16-17 22.630275987982934 27.752754576154732 26.404223848279155 23.21274558758318 18-19 22.417322826071963 26.801147586323044 28.617965689281334 22.163563898323662 20-21 23.381063548459572 25.959404364081674 28.47798486701198 22.181547220446774 22-23 23.350164450238413 25.680025123724455 28.330075361192105 22.639735064845034 24-25 22.94359630281064 25.888664381995476 28.522590587745096 22.645148727448795 26-27 22.811291165423718 26.02941177804977 28.333369779942032 22.825927276584483 28-29 22.624726602510357 26.25212965120542 28.479543370841327 22.643600375442897 30-31 23.125091647949915 25.996984510226095 28.411635768906336 22.46628807291765 32-33 23.04810401533329 26.223857800628203 28.09158847854335 22.636449705495156 34-35 23.117477663335652 26.16476961532992 28.280732317871866 22.437020403462565 36-37 23.085686685299624 26.065284211924926 28.21767962660508 22.63134947617037 38-39 23.078465934698634 26.114928020239393 28.248092256360525 22.558513788701454 40-41 22.998620265357317 25.887496954466034 28.415737050979402 22.698145729197243 42-43 22.816909127965086 26.08191991091251 28.15301168456626 22.948159276556147 44-45 22.80267653766294 26.30080204113357 28.061176292512386 22.83534512869111 46-47 22.850194356554297 26.17213327939229 27.709407684856522 23.268264679196886 48-49 23.201294506228866 26.283921043055486 27.542322833380084 22.97246161733557 50-51 22.970194306984943 26.38527549413044 27.481229582057065 23.16330061682755 52-53 22.887032216416603 26.516619070035613 27.34319135842301 23.253157355124777 54-55 22.836121200153617 26.460780626908864 27.37825028294894 23.324847889988582 56-57 23.027515834347636 26.386365795685666 27.17701919069303 23.409099179273664 58-59 22.801301039828214 26.44377153453134 27.477565157483337 23.277362268157113 60-61 22.916058878012542 26.593244133627692 27.296256917220934 23.194440071138835 62-63 22.973859577991316 26.468710877480568 27.232948706826615 23.324480837701504 64-65 22.677933903483687 26.349729648393165 27.289748057242047 23.682588390881104 66-67 22.79139550714772 26.30916269571137 27.233746312684364 23.66569548445655 68-69 22.618066933852166 26.33627542349652 27.284779103620366 23.760878539030948 70-71 22.641305648087187 26.11341555261124 27.35562660387404 23.88965219542753 72-73 22.78596552689629 26.416446310657278 27.050136796950124 23.74745136549631 74-75 22.79940499050061 26.5469266917225 26.986568364747505 23.667099953029386 76-77 22.69158838132942 26.42833002937084 26.94840010411021 23.931681485189525 78-79 22.57706538722398 26.375474396173143 27.040922165723906 24.006538050878973 80-81 22.58787427393769 26.589751156128216 26.9096668325408 23.912707737393294 82-83 22.782101262656834 26.290467518989217 27.219033126523602 23.708398091830347 84-85 22.84773705578191 26.47882706711233 26.777671044544427 23.895764832561333 86-87 22.51309019318268 26.62639501361932 26.968411820036938 23.892102973161062 88-89 22.579406328253125 26.23064695966246 27.000802023611953 24.189144688472464 90-91 22.719027869467524 26.164253045667635 27.002883711829206 24.113835373035634 92-93 22.689589475429482 26.270525116834254 26.90172092583855 24.138164481897714 94-95 22.32023009850475 26.149194880279232 27.06534025119474 24.465234770021276 96-97 22.411106685951047 26.0946667868084 27.02759820792814 24.466628319312413 98-99 22.233145704153934 26.191499740223207 26.947298207880426 24.62805634774244 100 22.242025895168176 26.047441435246288 26.920888703640433 24.789643965945103 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2077.0 1 1509.5 2 1860.5 3 2573.5 4 1753.0 5 1189.0 6 1775.0 7 3401.5 8 5288.5 9 6265.0 10 5476.0 11 4242.0 12 4128.5 13 4534.0 14 5107.0 15 5716.0 16 6237.0 17 6801.5 18 7451.0 19 8062.0 20 8638.5 21 9342.0 22 10381.5 23 12039.0 24 13957.5 25 16236.5 26 19602.5 27 23527.0 28 28499.0 29 35705.5 30 44977.5 31 54968.5 32 66918.5 33 79779.0 34 92419.0 35 104452.5 36 118353.0 37 136102.0 38 145540.0 39 146868.0 40 147752.0 41 149026.0 42 154419.0 43 163874.5 44 174101.0 45 188200.0 46 201949.5 47 211979.5 48 222424.5 49 228204.0 50 227482.0 51 226468.0 52 225376.0 53 222453.5 54 217544.5 55 204612.5 56 186650.5 57 165620.0 58 144658.5 59 126858.0 60 105617.5 61 84890.5 62 68471.5 63 53079.5 64 39313.5 65 28579.5 66 21112.0 67 16177.0 68 12320.0 69 9222.5 70 7199.5 71 5672.0 72 4380.0 73 3518.0 74 2758.0 75 2032.5 76 1374.0 77 843.0 78 526.0 79 337.0 80 213.0 81 133.0 82 81.5 83 45.0 84 21.5 85 17.5 86 13.5 87 9.0 88 6.5 89 3.5 90 4.5 91 3.0 92 1.0 93 2.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02863702462403017 2 0.0018510624281692504 3 9.073835432202208E-5 4 0.021323513265675186 5 5.988731385253457E-4 6 3.629534172880883E-5 7 5.4443012593213236E-5 8 0.003811010881524927 9 0.009890480621100406 10-11 9.709003912456362E-4 12-13 9.981218975422427E-5 14-15 9.073835432202208E-5 16-17 3.3573191099148166E-4 18-19 1.8147670864404414E-5 20-21 5.4443012593213236E-5 22-23 3.5387958185588604E-4 24-25 1.8147670864404414E-5 26-27 9.981218975422427E-5 28-29 0.0010344172392710515 30-31 0.002413640224965787 32-33 0.009209942963685241 34-35 0.017521576219582464 36-37 0.020234653013810923 38-39 0.01981725658392962 40-41 0.004201185805109622 42-43 0.004718394424745148 44-45 0.008520331470837871 46-47 0.01143303264457478 48-49 1.205368298813741 50-51 0.017213065814887588 52-53 0.01870117482576875 54-55 0.009454936520354701 56-57 0.007485914231566821 58-59 0.008139230382685379 60-61 0.005054126335736629 62-63 0.007531283408727832 64-65 0.018909873040709402 66-67 0.02901812571218266 68-69 0.028437400244521713 70-71 0.025805987969183077 72-73 0.023074763504090212 74-75 0.009454936520354701 76-77 0.014572579704116745 78-79 0.008166451888981987 80-81 0.002667707617067449 82-83 0.014082592590777826 84-85 0.023356052402488482 86-87 0.022920508301742776 88-89 0.03235729715123307 90-91 0.02050686807677699 92-93 0.013202430553854212 94-95 0.006551309182049994 96-97 0.0034571312996690405 98-99 0.014726834906464181 100 0.03322838535272448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5510349.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.033745913387904 #Duplication Level Percentage of deduplicated Percentage of total 1 74.44516441632048 43.9477692063345 2 14.784731075163377 17.455961153779302 3 4.849994564148787 8.589400403438159 4 2.1500786287693776 5.077087818583076 5 1.1081812999900362 3.2710046644789847 6 0.6778313301705727 2.4008953510454 7 0.43647317441350153 1.8036652533435542 8 0.299821683904148 1.415967768553726 9 0.2071166827458241 1.1004186261276676 >10 0.9799052155168705 9.785061703132452 >50 0.04318468629775214 1.725106292997861 >100 0.016393950532292437 1.6681268597417258 >500 6.584816885477855E-4 0.2677867303300483 >1k 3.4860775377126786E-4 0.45304893551066155 >5k 3.873419486344467E-5 0.22189405290434913 >10k+ 7.746838972688933E-5 0.8168051796987073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19347 0.35110298821363223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16374 0.2971499627337579 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9704 0.17610499806818045 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8147670864404414E-5 2 0.0 0.0 0.0 0.0 1.8147670864404414E-5 3 0.0 0.0 0.0 0.0 1.8147670864404414E-5 4 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 1.8147670864404414E-5 5 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 1.8147670864404414E-5 6 1.8147670864404414E-5 0.0 0.0 1.8147670864404414E-5 5.444301259321324E-5 7 1.8147670864404414E-5 0.0 0.0 3.629534172880883E-5 5.444301259321324E-5 8 1.8147670864404414E-5 0.0 0.0 3.629534172880883E-5 5.444301259321324E-5 9 1.8147670864404414E-5 0.0 0.0 7.259068345761766E-5 5.444301259321324E-5 10-11 1.8147670864404414E-5 0.0 0.0 7.259068345761766E-5 5.444301259321324E-5 12-13 1.8147670864404414E-5 9.073835432202207E-6 0.0 7.259068345761766E-5 5.444301259321324E-5 14-15 3.629534172880883E-5 1.8147670864404414E-5 0.0 7.259068345761766E-5 8.166451888981986E-5 16-17 3.629534172880883E-5 1.8147670864404414E-5 0.0 9.981218975422428E-5 1.270336960508309E-4 18-19 3.629534172880883E-5 1.8147670864404414E-5 0.0 1.9055054407624635E-4 1.270336960508309E-4 20-21 3.629534172880883E-5 1.8147670864404414E-5 0.0 2.63141227533864E-4 1.361075314830331E-4 22-23 3.629534172880883E-5 1.8147670864404414E-5 0.0 4.80913277906717E-4 1.8147670864404413E-4 24-25 3.629534172880883E-5 2.722150629660662E-5 0.0 8.801620369236141E-4 2.1777205037285297E-4 26-27 5.444301259321324E-5 3.629534172880883E-5 0.0 0.0019418007824912723 2.1777205037285297E-4 28-29 6.351684802541545E-5 3.629534172880883E-5 0.0 0.0044371055263468795 2.1777205037285297E-4 30-31 7.259068345761766E-5 3.629534172880883E-5 0.0 0.013011880009777967 2.3591972123725738E-4 32-33 7.259068345761766E-5 3.629534172880883E-5 0.0 0.03398151369359727 2.3591972123725738E-4 34-35 7.259068345761766E-5 3.629534172880883E-5 0.0 0.0670556438439743 2.3591972123725738E-4 36-37 7.259068345761766E-5 3.629534172880883E-5 0.0 0.12068201124828935 2.3591972123725738E-4 38-39 7.259068345761766E-5 4.536917716101103E-5 0.0 0.19824515652275382 2.3591972123725738E-4 40-41 7.259068345761766E-5 5.444301259321324E-5 0.0 0.2848912110648527 2.3591972123725738E-4 42-43 7.259068345761766E-5 5.444301259321324E-5 0.0 0.36985860605199417 2.449935566694596E-4 44-45 7.259068345761766E-5 5.444301259321324E-5 0.0 0.4780640935810055 2.722150629660662E-4 46-47 7.259068345761766E-5 5.444301259321324E-5 0.0 0.6062138713899973 2.9036273383047063E-4 48-49 7.259068345761766E-5 9.073835432202208E-5 0.0 0.7423214028730304 3.0851040469487506E-4 50-51 7.259068345761766E-5 1.270336960508309E-4 0.0 0.8914952573784347 3.175842401270773E-4 52-53 7.259068345761766E-5 1.270336960508309E-4 0.0 1.041077434478288 3.901749235846949E-4 54-55 7.259068345761766E-5 1.270336960508309E-4 0.0 1.2046242443083006 4.536917716101104E-4 56-57 7.259068345761766E-5 1.270336960508309E-4 0.0 1.3954288557766485 4.536917716101104E-4 58-59 7.259068345761766E-5 1.270336960508309E-4 0.0 1.6131555369723407 4.536917716101104E-4 60-61 7.259068345761766E-5 1.361075314830331E-4 0.0 1.8331597508615154 4.536917716101104E-4 62-63 7.259068345761766E-5 1.4518136691523531E-4 0.0 2.0588351118958164 4.7183944247451476E-4 64-65 7.259068345761766E-5 1.4518136691523531E-4 0.0 2.2877044630022527 4.7183944247451476E-4 66-67 7.259068345761766E-5 1.4518136691523531E-4 0.0 2.5486588961969563 4.7183944247451476E-4 68-69 7.259068345761766E-5 1.4518136691523531E-4 0.0 2.836843909523698 4.7183944247451476E-4 70-71 7.259068345761766E-5 1.4518136691523531E-4 0.0 3.145181911345361 4.7183944247451476E-4 72-73 7.259068345761766E-5 1.4518136691523531E-4 0.0 3.44641510002361 5.262824550677281E-4 74-75 7.259068345761766E-5 1.4518136691523531E-4 0.0 3.749898599889045 5.262824550677281E-4 76-77 7.259068345761766E-5 1.6332903777963972E-4 0.0 4.08568495389312 5.262824550677281E-4 78-79 7.259068345761766E-5 1.6332903777963972E-4 0.0 4.448411525295404 5.262824550677281E-4 80-81 7.259068345761766E-5 1.6332903777963972E-4 0.0 4.826146220502549 5.444301259321324E-4 82-83 7.259068345761766E-5 1.6332903777963972E-4 0.0 5.209497619842228 5.807254676609413E-4 84-85 7.259068345761766E-5 1.6332903777963972E-4 0.0 5.593048643561415 5.807254676609413E-4 86-87 7.259068345761766E-5 1.6332903777963972E-4 0.0 5.992705725172762 5.807254676609413E-4 88 7.259068345761766E-5 1.6332903777963972E-4 0.0 6.307168565911161 5.807254676609413E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9990 0.0 51.53106 1 GTATCAA 18040 0.0 41.048607 1 TATCAAC 23220 0.0 31.64422 2 CAACGCA 22955 0.0 31.57656 5 ATCAACG 22955 0.0 31.563282 3 TCAACGC 23805 0.0 30.534822 4 AACGCAG 24285 0.0 30.098095 6 GTGGTAT 4305 0.0 27.469791 1 ACGCAGA 27465 0.0 26.373844 7 GTACATG 23380 0.0 25.89245 1 GTACTAG 1965 0.0 25.553429 1 CGCAGAG 28355 0.0 25.413666 8 TACATGG 23940 0.0 25.067513 2 ACATGGG 24420 0.0 23.789602 3 GCAGAGT 30885 0.0 23.011406 9 CATGGGG 16965 0.0 22.543219 4 TACCTGG 4255 0.0 22.164675 2 TGGTATC 5190 0.0 21.968672 2 GAGTACT 17590 0.0 21.25315 12-13 ATGGGGG 11125 0.0 21.211998 5 >>END_MODULE