FastQCFastQC Report
Mon 6 Jun 2016
ERR1211280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5369498
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194100.3614863065411329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157820.2939194688218526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83050.15466995238661044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94600.052.900721
GTATCAA171850.041.519021
ATCAACG207050.033.4742473
CAACGCA207350.033.4028435
TATCAAC214850.032.8141482
TCAACGC216250.032.115934
AACGCAG219750.031.6878766
GTGGTAT42850.027.7703111
ACGCAGA249450.027.7295387
CGCAGAG256350.026.8900038
GTACATG223800.025.8077011
TACATGG229500.024.8788812
TGGTATC47150.024.1395152
GCAGAGT280600.024.0816089
ACATGGG235800.023.354893
GAGTACT155500.022.66434312-13
CATGGGG158100.021.5183434
TACCTGG34700.021.2796762
CAGAGTA274100.020.97603610-11
GTACTTT172950.020.13362914-15
Produced by FastQC (version 0.11.5)