FastQCFastQC Report
Mon 6 Jun 2016
ERR1211280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5369498
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169140.3150015141080228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144850.2697645105743591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82870.15433472551810243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95800.052.6104241
GTATCAA164900.039.471971
CAACGCA205600.030.9633675
ATCAACG205950.030.8475823
TATCAAC210350.030.6478772
TCAACGC216150.029.5007114
AACGCAG218450.029.442926
ACGCAGA247200.025.771927
GTACATG216100.025.5596391
CGCAGAG254150.025.0117848
GTGGTAT44050.024.6092911
TACATGG222250.024.4679642
ACATGGG221850.023.6453973
CATGGGG146950.022.7980084
GCAGAGT278600.022.6132019
GTACTAG18300.022.0536461
GAGTACT157000.021.76520212-13
TGGTATC47850.021.2781542
CAGAGTA272350.020.62495810-11
ATGGGGG94150.020.429375
Produced by FastQC (version 0.11.5)