FastQCFastQC Report
Mon 6 Jun 2016
ERR1211279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10225866
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363450.355422220475019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285040.27874411810207567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168400.16468042902185498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179150.051.985981
GTATCAA327250.041.941461
CAACGCA400300.033.3263855
ATCAACG401150.033.314183
TATCAAC421350.032.316182
GTACTAG50850.031.2653711
AACGCAG433550.031.215346
TCAACGC441200.030.3961374
ACGCAGA481650.027.6199637
GTACATG462350.027.0206051
CGCAGAG491750.027.0127418
TACATGG471500.026.1057682
GTGGTAT75950.025.8253441
ACATGGG475300.025.200453
GCATCGT55350.024.7886248
CTAGGCA69450.024.6279954
TAGGCAT66450.024.4674975
TAGTACT66000.024.3490564
GCAGAGT533100.024.3111029
CTAGTAC69050.023.6141363
Produced by FastQC (version 0.11.5)