##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211279_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10225866 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.454767449524567 31.0 31.0 34.0 29.0 34.0 2 31.479598207134732 31.0 31.0 34.0 28.0 34.0 3 31.52806706053062 33.0 31.0 34.0 28.0 34.0 4 35.13540281087196 37.0 35.0 37.0 32.0 37.0 5 35.18383714396414 37.0 35.0 37.0 32.0 37.0 6 35.24490981986269 37.0 35.0 37.0 32.0 37.0 7 35.17751454986795 37.0 35.0 37.0 32.0 37.0 8 35.18163957947425 37.0 35.0 37.0 32.0 37.0 9 36.74769755441739 39.0 37.0 39.0 32.0 39.0 10-11 36.67966727707952 39.0 37.0 39.0 32.0 39.0 12-13 36.59493215537931 39.0 37.0 39.0 32.0 39.0 14-15 37.73252236045338 40.0 37.0 41.0 32.0 41.0 16-17 37.73459866381977 40.0 37.0 41.0 32.0 41.0 18-19 37.74141133865827 40.0 37.0 41.0 32.0 41.0 20-21 37.70842322792026 40.0 37.0 41.0 32.0 41.0 22-23 37.587794618079286 40.0 37.0 41.0 32.0 41.0 24-25 37.47474028116543 40.0 37.0 41.0 31.5 41.0 26-27 37.32723590354108 40.0 37.0 41.0 31.0 41.0 28-29 37.19708120564067 40.0 36.0 41.0 31.0 41.0 30-31 37.012609445498306 39.0 36.0 41.0 30.0 41.0 32-33 36.89356241319806 39.0 36.0 41.0 30.0 41.0 34-35 36.755841607938144 39.0 35.5 41.0 30.0 41.0 36-37 36.660347348576636 39.0 35.0 41.0 30.0 41.0 38-39 36.50667449583243 39.0 35.0 41.0 30.0 41.0 40-41 36.33674683396008 39.0 35.0 40.5 29.0 41.0 42-43 36.18469296390154 39.0 35.0 40.0 28.5 41.0 44-45 36.02723583508722 39.0 35.0 40.0 28.0 41.0 46-47 35.84672530424318 39.0 35.0 40.0 27.0 41.0 48-49 35.597458249501805 38.0 34.5 40.0 26.0 41.0 50-51 34.91809686338546 37.5 33.5 39.5 25.5 40.5 52-53 35.154179069039245 38.0 34.0 40.0 26.0 40.5 54-55 35.50814659609269 38.0 34.0 40.0 26.0 41.0 56-57 35.393350744083676 38.0 34.0 40.0 26.0 41.0 58-59 35.16025459359628 38.0 34.0 40.0 26.0 41.0 60-61 34.89698657306873 37.5 34.0 40.0 26.0 41.0 62-63 34.61824998489126 37.0 33.5 40.0 26.0 41.0 64-65 34.341621237751404 36.5 33.0 40.0 25.0 41.0 66-67 34.12095836186393 36.0 33.0 39.0 25.0 41.0 68-69 33.78458856198585 35.5 33.0 39.0 24.0 41.0 70-71 33.456355432390765 35.0 32.5 39.0 24.0 40.5 72-73 33.10026652021452 35.0 32.0 38.0 23.0 40.0 74-75 32.729363654872856 35.0 32.0 37.0 22.5 39.5 76-77 32.38051144030246 35.0 31.0 37.0 22.0 39.0 78-79 32.01957873298946 35.0 31.0 36.0 21.0 39.0 80-81 31.69128546178876 35.0 31.0 36.0 20.0 38.0 82-83 31.372480873502546 34.5 31.0 35.0 20.0 37.0 84-85 31.07059685702903 34.0 30.0 35.0 19.5 37.0 86-87 30.81755613656584 34.0 30.0 35.0 19.0 36.0 88-89 30.56635037071677 34.0 30.0 35.0 18.0 36.0 90-91 30.3029641205938 34.0 29.0 35.0 18.0 36.0 92-93 30.059075436740514 34.0 29.0 35.0 17.0 35.0 94-95 29.840275826027842 34.0 29.0 35.0 16.5 35.0 96-97 29.636011659061445 34.0 29.0 35.0 15.0 35.0 98-99 29.40043043787196 34.0 27.5 35.0 14.0 35.0 100 27.698206880473496 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3547287749237569 1101 2 -0.343521792293739 1101 3 -0.3721381936034227 1101 4 -0.27220371387694087 1101 5 -0.33940024664860147 1101 6 -0.3522184614342052 1101 7 -0.37664825323616924 1101 8 -0.4106407379329511 1101 9 -0.41537479066852256 1101 10-11 -0.49103890077146417 1101 12-13 -0.4544985588026549 1101 14-15 -0.5761665018197917 1101 16-17 -0.572986756766916 1101 18-19 -0.6356633699541305 1101 20-21 -0.6822825168357483 1101 22-23 -0.6434325460599837 1101 24-25 -0.7150534490363682 1101 26-27 -0.746563180179713 1101 28-29 -0.803280456403229 1101 30-31 -0.7963384634687998 1101 32-33 -0.8596028662037867 1101 34-35 -0.8990127405230552 1101 36-37 -0.9951700848230018 1101 38-39 -0.8652391929433705 1101 40-41 -0.858551154316423 1101 42-43 -0.9466302124369008 1101 44-45 -1.0888360199105236 1101 46-47 -0.8973559584748401 1101 48-49 -0.917376384615693 1101 50-51 -0.8075149690494854 1101 52-53 -0.6867968321595299 1101 54-55 -0.6550335492916091 1101 56-57 -0.6523517376256933 1101 58-59 -0.7643954260899832 1101 60-61 -0.641830602631515 1101 62-63 -0.7367147385702495 1101 64-65 -0.7557877006084723 1101 66-67 -0.7648916676169151 1101 68-69 -0.7208269323301906 1101 70-71 -0.7091197544717716 1101 72-73 -0.8021589026304738 1101 74-75 -0.8580300360048305 1101 76-77 -0.88656141817075 1101 78-79 -0.8333217256127092 1101 80-81 -0.9620146211073504 1101 82-83 -1.1396824782211894 1101 84-85 -0.9921482377746109 1101 86-87 -1.0003622558365812 1101 88-89 -1.0570801878335558 1101 90-91 -1.068431920181883 1101 92-93 -1.087301060064437 1101 94-95 -1.1491136485440805 1101 96-97 -1.2359591564419112 1101 98-99 -1.2122925898294419 1101 100 -1.0367764925659806 1102 1 -0.43368409811712283 1102 2 -0.42005126059721576 1102 3 -0.4280755235973217 1102 4 -0.3049810832055613 1102 5 -0.2495536217229315 1102 6 -0.2367337336670161 1102 7 -0.3190902104859248 1102 8 -0.3005278649865133 1102 9 -0.4135908189474904 1102 10-11 -0.3831493117253899 1102 12-13 -0.39873996152715563 1102 14-15 -0.5163199005513448 1102 16-17 -0.6067134445068518 1102 18-19 -0.5601336364966585 1102 20-21 -0.6288876259650635 1102 22-23 -0.6021448777291951 1102 24-25 -0.6822151985737932 1102 26-27 -0.6416745216831856 1102 28-29 -0.6960877294247396 1102 30-31 -0.6225769720944214 1102 32-33 -0.7384985587163015 1102 34-35 -0.7138872013073936 1102 36-37 -0.7291552325299904 1102 38-39 -0.9277828984521008 1102 40-41 -0.9163790342258054 1102 42-43 -0.8398623377649415 1102 44-45 -0.8411776502657773 1102 46-47 -0.9292874373137963 1102 48-49 -0.8955084220354266 1102 50-51 -0.8776237468826835 1102 52-53 -0.7472127999725799 1102 54-55 -0.6454343059943781 1102 56-57 -0.7434361783583654 1102 58-59 -0.7285796729686354 1102 60-61 -0.8128837646556732 1102 62-63 -0.8417478388847925 1102 64-65 -0.8314910249597887 1102 66-67 -0.7560065465025616 1102 68-69 -0.7944925974756458 1102 70-71 -0.9236597649157119 1102 72-73 -0.9121985226819831 1102 74-75 -0.9885170025176002 1102 76-77 -0.8787068104296836 1102 78-79 -0.9146509664399325 1102 80-81 -0.8700154908240414 1102 82-83 -1.0210774957787088 1102 84-85 -1.068851260761896 1102 86-87 -1.038107665708072 1102 88-89 -1.1120951276099973 1102 90-91 -1.1865048565528404 1102 92-93 -1.1415813103597685 1102 94-95 -1.1637693452125433 1102 96-97 -1.0982957960891362 1102 98-99 -1.201791849503266 1102 100 -1.252696406982846 1103 1 -0.3860716504596873 1103 2 -0.4517833350473772 1103 3 -0.4444163793721465 1103 4 -0.2903416109774426 1103 5 -0.26125243236656814 1103 6 -0.3202562478672135 1103 7 -0.3667611922291343 1103 8 -0.38293022504623053 1103 9 -0.5712657411923701 1103 10-11 -0.5297346214226408 1103 12-13 -0.6284609843956943 1103 14-15 -0.6729518124693428 1103 16-17 -0.742289098568456 1103 18-19 -0.6751270938789773 1103 20-21 -0.8071219696118632 1103 22-23 -0.6914671352449844 1103 24-25 -0.7963770066949465 1103 26-27 -0.6937705071970299 1103 28-29 -0.823843132250694 1103 30-31 -0.8009647918977194 1103 32-33 -0.8297349132956668 1103 34-35 -0.9247469404379629 1103 36-37 -0.9358135010159998 1103 38-39 -1.0119149353365842 1103 40-41 -0.9671574618293093 1103 42-43 -0.978375840312637 1103 44-45 -1.0170323290651169 1103 46-47 -0.9866737248440458 1103 48-49 -1.009495547566054 1103 50-51 -1.054750541233723 1103 52-53 -0.89099858988736 1103 54-55 -0.8851098021113231 1103 56-57 -0.8857184212806644 1103 58-59 -0.8683331568286974 1103 60-61 -0.8492603727471391 1103 62-63 -0.932413188588626 1103 64-65 -0.8909781560084085 1103 66-67 -0.8219108959638177 1103 68-69 -0.8375375471092852 1103 70-71 -0.761725973999468 1103 72-73 -0.9158035623693905 1103 74-75 -0.8353666785460412 1103 76-77 -0.8180466714496397 1103 78-79 -0.8275541943405038 1103 80-81 -0.9017882470047986 1103 82-83 -0.9835696975453807 1103 84-85 -0.9922151573272977 1103 86-87 -1.0164942520759261 1103 88-89 -1.0733300907876213 1103 90-91 -1.1061370699051487 1103 92-93 -1.0792915218420127 1103 94-95 -1.3538712313084567 1103 96-97 -1.4423751652799517 1103 98-99 -1.5010163648334611 1103 100 -1.490295457800169 1104 1 -0.4611682460487785 1104 2 -0.47200458228835984 1104 3 -0.4796813648816922 1104 4 -0.37651371114852594 1104 5 -0.3008783861433102 1104 6 -0.29376909522050454 1104 7 -0.3685420054452635 1104 8 -0.3521242900405994 1104 9 -0.4250248828584091 1104 10-11 -0.43103837691766245 1104 12-13 -0.4798246560494661 1104 14-15 -0.6289864701400205 1104 16-17 -0.5873503172171226 1104 18-19 -0.6832066282698648 1104 20-21 -0.683793413832646 1104 22-23 -0.6009902065154051 1104 24-25 -0.7377213547689294 1104 26-27 -0.7142815797404864 1104 28-29 -0.6975323978272883 1104 30-31 -0.7628716874359185 1104 32-33 -0.8504494111564043 1104 34-35 -0.7009562136956404 1104 36-37 -0.7727323931718644 1104 38-39 -0.7810059450246598 1104 40-41 -0.855649397546749 1104 42-43 -0.8015543765119162 1104 44-45 -0.8001162422721535 1104 46-47 -0.9263482410913397 1104 48-49 -0.8420135570953633 1104 50-51 -0.7460299270653152 1104 52-53 -0.6454729530647043 1104 54-55 -0.7417551900436123 1104 56-57 -0.6278119505221085 1104 58-59 -0.6701148786643927 1104 60-61 -0.5731785901718496 1104 62-63 -0.6854448748299617 1104 64-65 -0.711353925363774 1104 66-67 -0.6778236282469337 1104 68-69 -0.6529829195625894 1104 70-71 -0.7338125320195701 1104 72-73 -0.7193564027749844 1104 74-75 -0.7670343905308386 1104 76-77 -0.6647280317926629 1104 78-79 -0.6766757255332116 1104 80-81 -0.7246198852353203 1104 82-83 -0.7826666108268121 1104 84-85 -0.7998715102695364 1104 86-87 -0.802672339400921 1104 88-89 -0.7648668917863901 1104 90-91 -0.7903437042720114 1104 92-93 -0.805049207120696 1104 94-95 -0.8010625057049943 1104 96-97 -0.8298953648257879 1104 98-99 -0.9289105473236958 1104 100 -0.9788052738525721 1105 1 -0.3319234410214271 1105 2 -0.39014874701560487 1105 3 -0.3512696086470619 1105 4 -0.275680518822945 1105 5 -0.20768744166357322 1105 6 -0.27826730672128264 1105 7 -0.21725222710625758 1105 8 -0.24310541849916234 1105 9 -0.35926059667365706 1105 10-11 -0.38906362626214985 1105 12-13 -0.4063222739621182 1105 14-15 -0.45803755364568843 1105 16-17 -0.5555189542976322 1105 18-19 -0.574589409525295 1105 20-21 -0.5835468323468831 1105 22-23 -0.572883082835844 1105 24-25 -0.5168864751347897 1105 26-27 -0.5453656866495038 1105 28-29 -0.6972013153550591 1105 30-31 -0.6309569717928625 1105 32-33 -0.5968747251863675 1105 34-35 -0.6560627621273838 1105 36-37 -0.7440904899643641 1105 38-39 -0.63734984242992 1105 40-41 -0.7866334939459705 1105 42-43 -0.7878808777340964 1105 44-45 -0.7361914094759783 1105 46-47 -0.7549062094697732 1105 48-49 -0.6946555027247925 1105 50-51 -0.6931361131563065 1105 52-53 -0.5682309418248153 1105 54-55 -0.5708107788863828 1105 56-57 -0.5617554154788067 1105 58-59 -0.49574250402001496 1105 60-61 -0.5381024276473596 1105 62-63 -0.5798992992384555 1105 64-65 -0.5466861265949348 1105 66-67 -0.5525863803843407 1105 68-69 -0.48636614385613086 1105 70-71 -0.48304841887166106 1105 72-73 -0.54525646147993 1105 74-75 -0.5906403156312408 1105 76-77 -0.5589283232919584 1105 78-79 -0.5157719565037446 1105 80-81 -0.5324785806396051 1105 82-83 -0.5463785046456628 1105 84-85 -0.5816532746105771 1105 86-87 -0.5767573134079171 1105 88-89 -0.6200440663611033 1105 90-91 -0.6316468327621543 1105 92-93 -0.6719780551689958 1105 94-95 -0.6569990887393118 1105 96-97 -0.7834730091676185 1105 98-99 -0.7505768649928655 1105 100 -0.8663867466943209 1106 1 -0.4244745963109615 1106 2 -0.4426857014534491 1106 3 -0.43022100351682013 1106 4 -0.32858207145240215 1106 5 -0.3505855511160405 1106 6 -0.3168153406176515 1106 7 -0.39083440659221935 1106 8 -0.3867556638255252 1106 9 -0.4830725992100682 1106 10-11 -0.5266439088460331 1106 12-13 -0.4752406274024281 1106 14-15 -0.6183408213738844 1106 16-17 -0.6041127725330355 1106 18-19 -0.585446222035749 1106 20-21 -0.5720687156745612 1106 22-23 -0.6065437320549805 1106 24-25 -0.5990192596329322 1106 26-27 -0.5668666256363153 1106 28-29 -0.6332539585567503 1106 30-31 -0.7007927809236776 1106 32-33 -0.6839802865030862 1106 34-35 -0.7238896194487054 1106 36-37 -0.7177945777455506 1106 38-39 -0.779057847500944 1106 40-41 -0.7510208585680473 1106 42-43 -0.7603152038651473 1106 44-45 -0.7735123834263646 1106 46-47 -0.7461428395549419 1106 48-49 -0.8157629438178517 1106 50-51 -0.8315312434043918 1106 52-53 -0.6510808332791029 1106 54-55 -0.7024191887109694 1106 56-57 -0.6826106348438756 1106 58-59 -0.6831929689165079 1106 60-61 -0.7775808516818188 1106 62-63 -0.7418467628473664 1106 64-65 -0.718345129488732 1106 66-67 -0.7668090786723525 1106 68-69 -0.7362081588177247 1106 70-71 -0.7335430488225256 1106 72-73 -0.6466366842138243 1106 74-75 -0.6718332457613414 1106 76-77 -0.724955996700313 1106 78-79 -0.6714637781922441 1106 80-81 -0.7008759732893282 1106 82-83 -0.7048154600299306 1106 84-85 -0.7357557704181517 1106 86-87 -0.8359552717481797 1106 88-89 -0.8157165246858185 1106 90-91 -0.7670812251428174 1106 92-93 -0.8356052393217261 1106 94-95 -0.830755511458694 1106 96-97 -0.9532678066353526 1106 98-99 -0.967388683652807 1106 100 -1.0314103103734915 1107 1 -0.4368196376557911 1107 2 -0.428057007257852 1107 3 -0.39422358650613987 1107 4 -0.26570103585064686 1107 5 -0.2637302904873593 1107 6 -0.28364641895684883 1107 7 -0.30655001798614023 1107 8 -0.2772206149793064 1107 9 -0.4149524235179811 1107 10-11 -0.4771870187459939 1107 12-13 -0.4520785555015294 1107 14-15 -0.5330647431414732 1107 16-17 -0.5617699454281251 1107 18-19 -0.6043176156687622 1107 20-21 -0.586453571058108 1107 22-23 -0.5946106025363278 1107 24-25 -0.6447315997924861 1107 26-27 -0.6749371051543847 1107 28-29 -0.724449946685283 1107 30-31 -0.6942364296074999 1107 32-33 -0.7465357787116247 1107 34-35 -0.8099696236853973 1107 36-37 -0.778036178344621 1107 38-39 -0.780358953182521 1107 40-41 -0.8098180599082738 1107 42-43 -0.8589118421433426 1107 44-45 -0.8112610114039853 1107 46-47 -0.7985985863574783 1107 48-49 -0.7533051418694043 1107 50-51 -0.7019494666860808 1107 52-53 -0.6130003526321346 1107 54-55 -0.548259425198772 1107 56-57 -0.5483611815388443 1107 58-59 -0.5956870028911396 1107 60-61 -0.587796463293671 1107 62-63 -0.5586801295250652 1107 64-65 -0.6147471857679179 1107 66-67 -0.5495119747499686 1107 68-69 -0.48304188291209726 1107 70-71 -0.5351931261247813 1107 72-73 -0.6045079802110607 1107 74-75 -0.5546636894679935 1107 76-77 -0.4761517516362588 1107 78-79 -0.5876551787469388 1107 80-81 -0.5447399025801047 1107 82-83 -0.5888565219759059 1107 84-85 -0.5965596920636962 1107 86-87 -0.6359686857705107 1107 88-89 -0.6306651721905929 1107 90-91 -0.6709902739306308 1107 92-93 -0.6202543890841064 1107 94-95 -0.5940058304094933 1107 96-97 -0.6756184881897305 1107 98-99 -0.6379436188143757 1107 100 -0.7165699557638376 1108 1 -0.4303734746772605 1108 2 -0.4426352855550242 1108 3 -0.43602625235747183 1108 4 -0.26736516697983603 1108 5 -0.3598945521217374 1108 6 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0.666079873660216 2311 20-21 0.7212130666342276 2311 22-23 0.8018804830736741 2311 24-25 0.8339266505232033 2311 26-27 0.9055027038098729 2311 28-29 0.8467514077364768 2311 30-31 0.8637888683648214 2311 32-33 0.9241809865994668 2311 34-35 0.9418738148122614 2311 36-37 0.9339543735939557 2311 38-39 0.9632599907173258 2311 40-41 1.0083864355680632 2311 42-43 1.0159278273376273 2311 44-45 1.0116708306852047 2311 46-47 1.065401128080957 2311 48-49 1.0255014592388818 2311 50-51 1.037276164743922 2311 52-53 0.9467907514960956 2311 54-55 0.9200807102689836 2311 56-57 0.8625384281522201 2311 58-59 0.8685083942832392 2311 60-61 0.9024796905792627 2311 62-63 0.8346061635355753 2311 64-65 0.8681520427655656 2311 66-67 0.8715875557204456 2311 68-69 0.9096199128664537 2311 70-71 0.8717717332450903 2311 72-73 0.8163078152857892 2311 74-75 0.8771345921760556 2311 76-77 0.9044654058129993 2311 78-79 0.9233517057607905 2311 80-81 0.962054659737575 2311 82-83 0.9555287564686132 2311 84-85 0.968357249171401 2311 86-87 0.969105175381916 2311 88-89 1.0245027150745116 2311 90-91 1.0472208568484902 2311 92-93 1.0455149669149897 2311 94-95 1.0957416780097802 2311 96-97 1.015142121632266 2311 98-99 1.0873307234474403 2311 100 1.1406935991111062 2312 1 0.437987291158759 2312 2 0.46962842570976093 2312 3 0.4639455879526295 2312 4 0.39335681892856655 2312 5 0.3717064280195359 2312 6 0.4157999082388599 2312 7 0.4581327930046868 2312 8 0.428559206054814 2312 9 0.5463401737091047 2312 10-11 0.5722155540518443 2312 12-13 0.6094460111111175 2312 14-15 0.7595352719447064 2312 16-17 0.7664986422443079 2312 18-19 0.7309824254163786 2312 20-21 0.7463418990399973 2312 22-23 0.7864040460140913 2312 24-25 0.7986223999100233 2312 26-27 0.8004673696105087 2312 28-29 0.787690909157547 2312 30-31 0.8616986692862412 2312 32-33 0.836468795726006 2312 34-35 0.8430986465817867 2312 36-37 0.864942739776609 2312 38-39 0.9435138207862437 2312 40-41 0.9124879610421033 2312 42-43 0.9252845112931283 2312 44-45 0.8992298980020266 2312 46-47 0.964486945591041 2312 48-49 0.9810324177686383 2312 50-51 0.9840410278843805 2312 52-53 0.7651400696249979 2312 54-55 0.7496188427486956 2312 56-57 0.738672500209077 2312 58-59 0.7347433693866066 2312 60-61 0.8033592746032383 2312 62-63 0.7806122164033624 2312 64-65 0.8004600214405357 2312 66-67 0.7873997629541236 2312 68-69 0.8458928389648008 2312 70-71 0.7974298794674581 2312 72-73 0.8078434324851358 2312 74-75 0.7727751582796216 2312 76-77 0.8074927325913066 2312 78-79 0.8587028312533498 2312 80-81 0.9011797717869428 2312 82-83 0.8926202436798611 2312 84-85 0.878141965638374 2312 86-87 0.9145006414138983 2312 88-89 0.9348656263603843 2312 90-91 0.9679627069401242 2312 92-93 0.9955449274737092 2312 94-95 1.0553093007111158 2312 96-97 1.0411455384946002 2312 98-99 1.1314501600791864 2312 100 1.276063518770247 2313 1 0.44113701237105474 2313 2 0.460040366471528 2313 3 0.4982963932941473 2313 4 0.4208924526104667 2313 5 0.4024280861261005 2313 6 0.3872571067444923 2313 7 0.4495947456588141 2313 8 0.4425810789953317 2313 9 0.5625271017518187 2313 10-11 0.5634254631810194 2313 12-13 0.5814582085152722 2313 14-15 0.7227792547449141 2313 16-17 0.7354196193682938 2313 18-19 0.6894153206299265 2313 20-21 0.7041401798596141 2313 22-23 0.6473342591225517 2313 24-25 0.6508442829916845 2313 26-27 0.7159709125664335 2313 28-29 0.7141576344087781 2313 30-31 0.767236211755403 2313 32-33 0.8233700935599018 2313 34-35 0.8119780689315022 2313 36-37 0.8614410521070042 2313 38-39 0.8707084935794924 2313 40-41 0.8252613451955568 2313 42-43 0.700027610335475 2313 44-45 0.7382434460114808 2313 46-47 0.7954384306349098 2313 48-49 0.8531442963166924 2313 50-51 0.8304048478192172 2313 52-53 0.6790158939385336 2313 54-55 0.6502062972082499 2313 56-57 0.6768729757580942 2313 58-59 0.682917815357655 2313 60-61 0.7002480990866289 2313 62-63 0.6804418945890447 2313 64-65 0.6806636519238936 2313 66-67 0.6096720059637875 2313 68-69 0.62168337827638 2313 70-71 0.6077873592611382 2313 72-73 0.6159377301875821 2313 74-75 0.6044027111641483 2313 76-77 0.6849562776671547 2313 78-79 0.6537868892276855 2313 80-81 0.673592250017947 2313 82-83 0.6972248697615306 2313 84-85 0.7393513955232187 2313 86-87 0.7791376396185932 2313 88-89 0.7412743229357162 2313 90-91 0.8967187393180858 2313 92-93 0.890060304536135 2313 94-95 0.91672891186996 2313 96-97 0.9384358681210578 2313 98-99 0.9891910573648985 2313 100 1.030393447283874 2314 1 0.31506891500043466 2314 2 0.23695784443698287 2314 3 0.2692438950935703 2314 4 0.2456648735274456 2314 5 0.26945112118217196 2314 6 0.27552439848624033 2314 7 0.32613238477912887 2314 8 0.3002948633256537 2314 9 0.37863897389837575 2314 10-11 0.45986192861573016 2314 12-13 0.4303996910531822 2314 14-15 0.5207073264811086 2314 16-17 0.5475603093395947 2314 18-19 0.548879170525403 2314 20-21 0.5082969930008332 2314 22-23 0.5837858494792911 2314 24-25 0.6074973202192169 2314 26-27 0.6650083032704828 2314 28-29 0.663974236513738 2314 30-31 0.6551600462138154 2314 32-33 0.6985883548029364 2314 34-35 0.6160642230438782 2314 36-37 0.666462194040335 2314 38-39 0.7410426273972845 2314 40-41 0.6984324292446686 2314 42-43 0.5787663130204805 2314 44-45 0.6968180184644979 2314 46-47 0.6103300753109195 2314 48-49 0.5506447819667315 2314 50-51 0.7302662574696868 2314 52-53 0.6807021746286424 2314 54-55 0.590310975477415 2314 56-57 0.5619742428828047 2314 58-59 0.5878243702241406 2314 60-61 0.43187752339011354 2314 62-63 0.5684052657223546 2314 64-65 0.6206410897437493 2314 66-67 0.5789445625664058 2314 68-69 0.607356602437541 2314 70-71 0.6083449306558038 2314 72-73 0.6975068608903285 2314 74-75 0.6966722340284477 2314 76-77 0.6870508261503687 2314 78-79 0.7752404981982117 2314 80-81 0.7660394169321947 2314 82-83 0.7484256561372824 2314 84-85 0.7870035509381488 2314 86-87 0.7908079357744278 2314 88-89 0.6073078880428291 2314 90-91 0.6081401444560406 2314 92-93 0.8166844379365408 2314 94-95 0.9018699345173715 2314 96-97 0.860318512787213 2314 98-99 0.8053423358381266 2314 100 0.9737274719659013 2315 1 0.34038190765584986 2315 2 0.37151956375801376 2315 3 0.39114363404791064 2315 4 0.26138289966429085 2315 5 0.2518349147339336 2315 6 0.2790308112436861 2315 7 0.29018841866888323 2315 8 0.30282646872424124 2315 9 0.12841308082482783 2315 10-11 0.3254731575704213 2315 12-13 0.33711717454386303 2315 14-15 0.49160488315961715 2315 16-17 0.3281394458873521 2315 18-19 0.4912405759526237 2315 20-21 0.39956387750537203 2315 22-23 0.3322991275236902 2315 24-25 0.5117687804058662 2315 26-27 0.6280641673881746 2315 28-29 0.48080071837101457 2315 30-31 0.5560505608861774 2315 32-33 0.5703437111411915 2315 34-35 0.5042801876483551 2315 36-37 0.6735539924197838 2315 38-39 0.6868087965709435 2315 40-41 0.7453033751358973 2315 42-43 0.7625053325586464 2315 44-45 0.7540866178006667 2315 46-47 0.796563518260605 2315 48-49 0.6753664255960032 2315 50-51 0.6439858214081013 2315 52-53 0.37519630355773614 2315 54-55 0.5100877196082649 2315 56-57 0.5102266279937382 2315 58-59 0.5315605596873354 2315 60-61 0.6636915918565194 2315 62-63 0.6547580924501517 2315 64-65 0.6300931806587968 2315 66-67 0.5428260871046433 2315 68-69 0.5885011687469657 2315 70-71 0.5372482374725251 2315 72-73 0.6157584880347642 2315 74-75 0.3611120012975917 2315 76-77 0.5818075834426395 2315 78-79 0.6476104791351069 2315 80-81 0.6970450366834058 2315 82-83 0.6332152688714103 2315 84-85 0.5810592791003337 2315 86-87 0.5676415784418012 2315 88-89 0.49431955025214336 2315 90-91 0.6950359755173103 2315 92-93 0.8279157666760035 2315 94-95 0.825627104298583 2315 96-97 0.8529217335852728 2315 98-99 0.9577236103136499 2315 100 1.0094774727194071 2316 1 0.12036489782484949 2316 2 0.15048419534139867 2316 3 0.1159165380661129 2316 4 0.04178544078121149 2316 5 -0.004099273483966215 2316 6 -0.12787324307799963 2316 7 -0.06656051130741503 2316 8 0.018082822669249765 2316 9 -0.013552179577253298 2316 10-11 0.07065992402180399 2316 12-13 0.08026380692776058 2316 14-15 -0.08297208861731775 2316 16-17 0.13378137832359727 2316 18-19 0.15825687298337954 2316 20-21 0.19689496454275002 2316 22-23 0.10927440505800234 2316 24-25 0.1363911447575461 2316 26-27 0.05744597867581547 2316 28-29 0.09708673410421653 2316 30-31 -0.44702658061031997 2316 32-33 -0.25577550900726465 2316 34-35 -0.44031143643838533 2316 36-37 -0.20708670636596338 2316 38-39 -0.12676193384016443 2316 40-41 0.0985507343279366 2316 42-43 0.15691451771369458 2316 44-45 0.046431458325741914 2316 46-47 -0.06931567412014772 2316 48-49 0.044879679256276006 2316 50-51 0.18377294355700968 2316 52-53 -0.17821344877464185 2316 54-55 -0.13533252377575167 2316 56-57 -0.10468657762690015 2316 58-59 -0.5010698869825205 2316 60-61 -0.04413197669174451 2316 62-63 -0.07744095528693862 2316 64-65 -0.3269001438511694 2316 66-67 -0.5960554518742072 2316 68-69 -0.5789883752760474 2316 70-71 -0.3911421524477561 2316 72-73 -0.6169341228186767 2316 74-75 -0.4004236943177162 2316 76-77 -0.08805515283641796 2316 78-79 -0.04544354163786579 2316 80-81 -0.07373212827303632 2316 82-83 0.0912886413529499 2316 84-85 0.15992717958582503 2316 86-87 0.3159807473033318 2316 88-89 0.2150237473204939 2316 90-91 -0.3254815807975291 2316 92-93 -0.3087127546208386 2316 94-95 0.06684316285469194 2316 96-97 0.2818663125160725 2316 98-99 0.39544697789901306 2316 100 0.2594878880778637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 262.0 1 742.0 2 460.0 3 436.0 4 359.0 5 355.0 6 448.0 7 1048.0 8 2660.0 9 4767.0 10 7617.0 11 12641.0 12 18926.0 13 23424.0 14 27347.0 15 30794.0 16 35948.0 17 43762.0 18 52868.0 19 64012.0 20 78451.0 21 97748.0 22 108247.0 23 108257.0 24 106201.0 25 110091.0 26 121631.0 27 138799.0 28 163019.0 29 195519.0 30 237818.0 31 295346.0 32 373701.0 33 488168.0 34 681020.0 35 945367.0 36 1398411.0 37 1982504.0 38 1807126.0 39 459303.0 40 263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6623009575985 19.72110612657038 13.424680098679625 23.191912817151497 2 15.63691161002872 19.953784813130408 41.08418314402676 23.325120432814114 3 19.088715553708095 25.9797442355185 29.181475413844538 25.750064796928868 4 12.565715764007651 16.532619598020176 38.70490882122661 32.19675581674556 5 13.395839642063207 37.99120064225804 34.574294732905 14.038664982773744 6 30.73479092307081 37.2911661937591 17.986450860890038 13.98759202228004 7 27.648071603782594 32.32564364665532 22.218381596722715 17.807903152839373 8 26.11137372695448 35.27340787623176 19.9270412751234 18.688177121690355 9 26.675925914150966 15.759760287356226 20.75685837606638 36.80745542242643 10-11 25.236643763385192 26.190144975854313 27.678750661800354 20.894460598960137 12-13 25.787818288439606 23.83888022123541 28.073761962099574 22.299539528225413 14-15 23.251033546706772 25.17467153329298 25.79373085600044 25.780564063999805 16-17 22.184429471478026 28.143628366251622 26.747924912379972 22.924017249890383 18-19 21.841646107958613 27.49319284492883 28.98282587070614 21.682335176406408 20-21 23.04161275410245 26.471948752693592 28.777358435186294 21.709080058017665 22-23 23.142489919384428 26.332268281834974 28.383319137212403 22.141922661568195 24-25 22.641283626837453 26.457160102149825 28.826114649743857 22.075441621268865 26-27 22.434667939472074 26.737788387098792 28.468272428890025 22.35927124453911 28-29 22.120811433937014 26.876456022346424 28.673941154597436 22.328791389119125 30-31 22.718673141507974 26.628790984428562 28.558338438888025 22.094197435175435 32-33 22.658737544811043 26.9584012158813 28.14389037501495 22.238970864292707 34-35 22.72205650690016 26.818267936181318 28.572894715202306 21.886780841716217 36-37 22.649630574492168 26.608541913495337 28.378508003938073 22.363319508074426 38-39 22.695681422679034 26.68671786695198 28.602604771059898 22.01499593930908 40-41 22.62176490870885 26.267589069092907 28.80569765880895 22.304948363389297 42-43 22.46482060133374 26.386351816980536 28.44373571138877 22.705091870296958 44-45 22.511632285149112 26.89842651033794 28.278498120125654 22.311443084387296 46-47 22.494025837619326 26.695375132581812 27.92920117927908 22.881397850519786 48-49 22.76226236674834 26.655752140214105 27.961302437685752 22.620683055351794 50-51 22.74800943647021 26.70026272102112 27.678849847270353 22.872877995238323 52-53 22.40288681941169 27.070439295861952 27.622631136877672 22.904042747848685 54-55 22.405696444594597 26.99711301320038 27.63636039872451 22.960830143480514 56-57 22.777192577940717 26.79882903934289 27.379050152416955 23.04492823029943 58-59 22.382849453183542 26.8835652040718 27.865773751880496 22.86781159086416 60-61 22.553534906437925 27.153758293864826 27.46707990209776 22.825626897599484 62-63 22.613271836311437 26.85326305754568 27.44767904041789 23.085786065724992 64-65 22.205072273255144 26.789777099694344 27.563286002409516 23.441864624640996 66-67 22.59874125839915 26.579407613601063 27.41478544399627 23.407065684003513 68-69 22.264032547670055 26.747595527126883 27.541835154303634 23.446536770899424 70-71 22.395289564152296 26.42804570591665 27.607606732951428 23.56905799697963 72-73 22.675944002119063 26.666814095775365 27.43889414077908 23.218347761326495 74-75 22.66608992299425 26.80553848083584 27.34864257731236 23.17972901885755 76-77 22.48641310994731 26.697869598339185 27.303103346857117 23.512613944856394 78-79 22.41157540852918 26.63035187440671 27.354328629886687 23.603744087177418 80-81 22.35431323475262 26.94807510848611 27.3501589263909 23.347452730370367 82-83 22.783603193995436 26.480940420618754 27.782384808243794 22.95307157714202 84-85 22.821998930912756 26.615482704240822 27.240163903227156 23.32235446161927 86-87 22.251557881012747 26.949033593968814 27.515117714039704 23.28429081097873 88-89 22.664823497684196 26.3390080549107 27.460854676165503 23.535313771239597 90-91 22.77651515207117 26.31991603306636 27.499077801278617 23.404491013583854 92-93 22.88114801425672 26.432918654106246 27.32673800245441 23.35919532918263 94-95 22.313143306771437 26.35554121261514 27.623974752292717 23.707340728320702 96-97 22.517589029952997 26.24529001140389 27.48527833447816 23.751842624164954 98-99 22.347272466934893 26.44385467159116 27.32792182974826 23.88095103172569 100 22.789434069859812 26.221550916929985 26.796850544196925 24.192164469013278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2515.0 1 1735.0 2 1523.0 3 1986.0 4 1562.0 5 1401.0 6 2106.5 7 3835.0 8 6161.0 9 7486.0 10 6808.0 11 5923.0 12 6340.0 13 7225.0 14 8266.0 15 9352.5 16 10274.5 17 11182.5 18 12081.0 19 12921.5 20 13782.0 21 14980.0 22 16740.5 23 19381.0 24 22567.5 25 26577.0 26 32963.5 27 40974.5 28 50759.0 29 66212.5 30 87508.5 31 109511.5 32 138263.5 33 171842.5 34 203593.0 35 234882.0 36 268897.5 37 310008.5 38 327663.5 39 321717.0 40 311975.0 41 299828.5 42 298662.5 43 313055.5 44 332982.0 45 363719.0 46 391552.0 47 407016.0 48 421558.0 49 427485.5 50 421780.5 51 416876.0 52 408742.0 53 392347.0 54 374357.0 55 341832.0 56 306758.5 57 264369.5 58 222259.5 59 196998.0 60 164109.0 61 128215.5 62 101513.0 63 80002.5 64 59757.5 65 41706.5 66 30587.5 67 23519.0 68 18394.5 69 14543.0 70 11504.5 71 9100.5 72 7603.5 73 6074.0 74 4529.0 75 3430.0 76 2525.5 77 1787.0 78 1313.0 79 895.0 80 627.5 81 460.0 82 322.5 83 227.5 84 159.5 85 118.0 86 87.0 87 72.5 88 52.0 89 43.5 90 43.0 91 33.5 92 22.5 93 18.5 94 18.5 95 11.0 96 9.5 97 10.0 98 7.0 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024242445578692307 2 0.10798107465910467 3 0.015803062547465416 4 0.01682987044813613 5 0.018951940109522267 6 0.04596187745859373 7 0.046255251144499646 8 0.03835371987076693 9 0.0322026515896062 10-11 0.03667171073823968 12-13 0.03552266380177483 14-15 0.03108294202173195 16-17 0.0380212296934069 18-19 0.036353889245174936 20-21 0.04734073378235154 22-23 0.04658774132185968 24-25 0.0660970914346032 26-27 0.057911965597828095 28-29 0.05055317564302133 30-31 0.048621798877474046 32-33 0.04374690613000405 34-35 0.045859196668526656 36-37 0.04025575926772364 38-39 0.04595209833573019 40-41 0.054743529790044196 42-43 0.05623973558816436 44-45 0.059007227358543524 46-47 0.06094838324695435 48-49 0.06016605341787189 50-51 0.05397097908382527 52-53 0.06445908835496182 54-55 0.05060696081877075 56-57 0.05584368111219138 58-59 0.053594482853579345 60-61 0.04946280344373768 62-63 0.05623484602673261 64-65 0.054342585752639436 66-67 0.05223029521411683 68-69 0.057721272701989255 70-71 0.057892407352101036 72-73 0.06126620474001908 74-75 0.05149197143792027 76-77 0.05233786556561566 78-79 0.061989859831920346 80-81 0.06214632579773684 82-83 0.052567674952908636 84-85 0.05801953594932693 86-87 0.055286271108970136 88-89 0.06366208984158409 90-91 0.06925085855809181 92-93 0.07359767867093114 94-95 0.0773381931662316 96-97 0.0725904290159875 98-99 0.08188059573634154 100 0.12641472125685982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0225866E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.82927671740523 #Duplication Level Percentage of deduplicated Percentage of total 1 69.42637453038195 35.98318777021381 2 15.92469409891987 16.507307541858964 3 6.2398442696914405 9.702198459820591 4 3.0712290282212362 6.367183166648241 5 1.6708014156129058 4.3298214454816835 6 0.9977369429292174 3.1027190463753813 7 0.6459796147717866 2.3436459345466827 8 0.42425469948643124 1.7591051374673472 9 0.3077210148388956 1.4354061866861292 >10 1.2045933352822684 10.26396851337316 >50 0.05069183717686971 1.7962449752691043 >100 0.03237327317482853 3.2868216085412434 >500 0.002319811032217831 0.8145307858704943 >1k 0.0012729751361412543 1.0131926369105915 >5k 2.828833597694713E-5 0.09697208651184347 >10k+ 8.486500793084139E-5 1.1976947044248536 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36345 0.355422220475019 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28504 0.27874411810207567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16840 0.16468042902185498 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.77912286353058E-6 2 0.0 0.0 0.0 0.0 9.77912286353058E-6 3 0.0 0.0 0.0 0.0 9.77912286353058E-6 4 0.0 0.0 0.0 9.77912286353058E-6 9.77912286353058E-6 5 0.0 0.0 0.0 9.77912286353058E-6 9.77912286353058E-6 6 0.0 0.0 0.0 9.77912286353058E-6 9.77912286353058E-6 7 0.0 0.0 0.0 9.77912286353058E-6 9.77912286353058E-6 8 0.0 0.0 0.0 9.77912286353058E-6 9.77912286353058E-6 9 0.0 0.0 0.0 1.955824572706116E-5 9.77912286353058E-6 10-11 0.0 0.0 0.0 3.422693002235703E-5 9.77912286353058E-6 12-13 0.0 0.0 0.0 3.911649145412232E-5 9.77912286353058E-6 14-15 0.0 0.0 0.0 3.911649145412232E-5 1.955824572706116E-5 16-17 0.0 0.0 0.0 5.378517574941819E-5 3.911649145412232E-5 18-19 0.0 0.0 0.0 7.823298290824464E-5 6.845386004471407E-5 20-21 0.0 0.0 0.0 2.0536158013414218E-4 7.823298290824464E-5 22-23 0.0 0.0 0.0 6.307534246977225E-4 8.801210577177522E-5 24-25 9.77912286353058E-6 0.0 0.0 0.0014375310609389953 1.1734947436236696E-4 26-27 9.77912286353058E-6 0.0 0.0 0.0027723813318109194 1.466868429529587E-4 28-29 9.77912286353058E-6 0.0 0.0 0.007026299777446722 1.466868429529587E-4 30-31 9.77912286353058E-6 0.0 0.0 0.02103000371802251 1.5646596581648928E-4 32-33 9.77912286353058E-6 0.0 0.0 0.05099812573331197 1.8580333440708103E-4 34-35 9.77912286353058E-6 0.0 0.0 0.09785968249535051 1.8580333440708103E-4 36-37 9.77912286353058E-6 0.0 0.0 0.17426396942811495 1.8580333440708103E-4 38-39 9.77912286353058E-6 0.0 0.0 0.2799371710914264 1.8580333440708103E-4 40-41 9.77912286353058E-6 0.0 0.0 0.3915316316486056 1.8580333440708103E-4 42-43 9.77912286353058E-6 0.0 0.0 0.5042213539665003 1.8580333440708103E-4 44-45 9.77912286353058E-6 0.0 0.0 0.6449820484641594 1.8580333440708103E-4 46-47 9.77912286353058E-6 0.0 0.0 0.8088850372183637 1.8580333440708103E-4 48-49 9.77912286353058E-6 0.0 0.0 0.9862881050856719 2.1514070299767277E-4 50-51 9.77912286353058E-6 0.0 0.0 1.1680233243815241 2.1514070299767277E-4 52-53 9.77912286353058E-6 0.0 0.0 1.3446342832968865 2.395885101564992E-4 54-55 9.77912286353058E-6 0.0 0.0 1.5399184773201604 2.6403631731532564E-4 56-57 9.77912286353058E-6 9.77912286353058E-6 0.0 1.76696037284275 2.738154401788562E-4 58-59 9.77912286353058E-6 9.77912286353058E-6 0.0 2.0229142451113677 2.884841244741521E-4 60-61 9.77912286353058E-6 9.77912286353058E-6 0.0 2.2869603415495567 2.933736859059174E-4 62-63 9.77912286353058E-6 9.77912286353058E-6 0.0 2.559504495756154 3.1293193163297857E-4 64-65 9.77912286353058E-6 9.77912286353058E-6 0.0 2.833222144706375 3.1293193163297857E-4 66-67 9.77912286353058E-6 9.77912286353058E-6 0.0 3.140325719112689 3.1293193163297857E-4 68-69 9.77912286353058E-6 9.77912286353058E-6 0.0 3.4740578450763975 3.1293193163297857E-4 70-71 9.77912286353058E-6 9.77912286353058E-6 0.0 3.835728925061212 3.2271105449650913E-4 72-73 9.77912286353058E-6 9.77912286353058E-6 0.0 4.199248259267234 3.813857916776926E-4 74-75 9.77912286353058E-6 9.77912286353058E-6 0.0 4.556210691593259 3.813857916776926E-4 76-77 9.77912286353058E-6 9.77912286353058E-6 0.0 4.943336828391844 3.813857916776926E-4 78-79 9.77912286353058E-6 9.77912286353058E-6 0.0 5.36095915984035 3.813857916776926E-4 80-81 9.77912286353058E-6 9.77912286353058E-6 0.0 5.808476269882668 3.960544759729885E-4 82-83 9.77912286353058E-6 9.77912286353058E-6 0.0 6.262056436100375 4.6450833601770257E-4 84-85 9.77912286353058E-6 1.466868429529587E-5 0.0 6.697584341512005 4.7428745888123314E-4 86-87 9.77912286353058E-6 1.955824572706116E-5 0.0 7.14855348192515 4.840665817447637E-4 88 9.77912286353058E-6 1.955824572706116E-5 0.0 7.503775230381466 4.987352660400596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17915 0.0 51.98598 1 GTATCAA 32725 0.0 41.94146 1 CAACGCA 40030 0.0 33.326385 5 ATCAACG 40115 0.0 33.31418 3 TATCAAC 42135 0.0 32.31618 2 GTACTAG 5085 0.0 31.265371 1 AACGCAG 43355 0.0 31.21534 6 TCAACGC 44120 0.0 30.396137 4 ACGCAGA 48165 0.0 27.619963 7 GTACATG 46235 0.0 27.020605 1 CGCAGAG 49175 0.0 27.012741 8 TACATGG 47150 0.0 26.105768 2 GTGGTAT 7595 0.0 25.825344 1 ACATGGG 47530 0.0 25.20045 3 GCATCGT 5535 0.0 24.788624 8 CTAGGCA 6945 0.0 24.627995 4 TAGGCAT 6645 0.0 24.467497 5 TAGTACT 6600 0.0 24.349056 4 GCAGAGT 53310 0.0 24.311102 9 CTAGTAC 6905 0.0 23.614136 3 >>END_MODULE