FastQCFastQC Report
Mon 6 Jun 2016
ERR1211279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10225866
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314800.3078467877439427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258980.25325972391971496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163420.15981042583581676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176650.051.407991
GTATCAA318850.040.535991
ATCAACG393450.032.152053
CAACGCA396100.031.9534455
TATCAAC417950.030.7813322
AACGCAG429500.029.9164076
TCAACGC431750.029.3979784
GTACTAG49150.026.6405941
ACGCAGA478950.026.377217
CGCAGAG490100.025.7292588
GTACATG461800.025.3762761
TACATGG467750.024.6253262
ACATGGG467250.023.89043
GTGGTAT74850.023.8889141
GCAGAGT542550.023.0159939
TAGTACT68750.022.9323774
CTAGTAC74850.021.8155273
CTGTGCG72200.021.700559
GAGTACT285050.021.47346312-13
CATGGGG310800.021.2571074
Produced by FastQC (version 0.11.5)