FastQCFastQC Report
Mon 6 Jun 2016
ERR1211278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7868313
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283160.3598738382674914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220960.2808225854767089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128060.16275407447568493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128150.051.780091
GTATCAA248550.043.423351
CAACGCA301350.035.0530555
ATCAACG301950.035.0305373
TATCAAC314850.033.921372
TCAACGC315850.033.5186584
AACGCAG321750.033.0640376
ACGCAGA361400.029.1915137
CGCAGAG368000.028.5767638
GTGGTAT58350.027.6444341
GCAGAGT403350.025.548849
GTACTAG30300.025.2987691
GTACATG320050.025.1706241
TACATGG324050.024.4103452
TGGTATC70200.022.9782522
ACATGGG330900.022.9625193
GAGTACT236000.021.47434812-13
CAGAGTA397750.020.91213410-11
AGAGTAC381200.020.72288110-11
CATGGGG219650.020.34494
Produced by FastQC (version 0.11.5)