FastQCFastQC Report
Mon 6 Jun 2016
ERR1211278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7868313
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245720.312290576137477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208110.26449125752877395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125350.15930988002129554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131200.053.6168331
GTATCAA238350.040.913231
CAACGCA299250.031.9337145
ATCAACG298700.031.9196663
TATCAAC314800.030.7619922
TCAACGC312800.030.5556414
AACGCAG322600.030.029326
GTGGTAT59050.027.3383261
GTACTAG30750.027.164911
ACGCAGA362700.026.3471267
CGCAGAG373350.025.6206938
TGGTATC65700.023.7803552
GTACATG311600.023.2080441
GCAGAGT409400.023.0886069
TACATGG322150.022.1810072
ACATGGG322400.021.5249943
GAGTACT236350.020.75946812-13
CAGAGTA402400.020.2128310-11
AGAGTAC385600.019.97432510-11
GCATCGT34250.019.8608238
Produced by FastQC (version 0.11.5)