##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211278_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7868313 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.976951857405773 31.0 31.0 34.0 30.0 34.0 2 32.121340368640645 33.0 31.0 34.0 30.0 34.0 3 32.180374497049115 34.0 31.0 34.0 30.0 34.0 4 35.85467113471464 37.0 35.0 37.0 35.0 37.0 5 35.75033631732749 37.0 35.0 37.0 35.0 37.0 6 35.765572493112565 37.0 35.0 37.0 33.0 37.0 7 35.680282545953624 37.0 35.0 37.0 33.0 37.0 8 35.66024470556776 37.0 35.0 37.0 33.0 37.0 9 37.25040107072507 39.0 37.0 39.0 34.0 39.0 10-11 37.193149344719764 39.0 37.0 39.0 33.0 39.0 12-13 37.12172996930854 39.0 37.0 39.0 33.0 39.0 14-15 38.31186049665284 40.0 38.0 41.0 33.0 41.0 16-17 38.29245938741887 40.0 38.0 41.0 33.0 41.0 18-19 38.30055648523388 40.0 38.0 41.0 33.5 41.0 20-21 38.26834786567336 40.0 38.0 41.0 34.0 41.0 22-23 38.15308128947082 40.0 37.5 41.0 33.0 41.0 24-25 38.051882531871826 40.0 37.0 41.0 33.0 41.0 26-27 37.944144824945326 40.0 37.0 41.0 33.0 41.0 28-29 37.83706780602144 40.0 37.0 41.0 33.0 41.0 30-31 37.838930085775694 40.0 37.0 41.0 33.0 41.0 32-33 37.764364101936465 40.0 37.0 41.0 33.0 41.0 34-35 37.623828957999 40.0 37.0 41.0 32.5 41.0 36-37 37.46397296345481 40.0 36.0 41.0 32.0 41.0 38-39 37.33897393253166 40.0 36.0 41.0 31.0 41.0 40-41 37.22839679865302 39.0 36.0 41.0 31.0 41.0 42-43 37.111347375225165 39.0 36.0 41.0 31.0 41.0 44-45 36.94384482417006 39.0 35.5 41.0 31.0 41.0 46-47 36.69217213397586 39.0 35.0 41.0 30.0 41.0 48-49 35.15113309549328 38.5 34.5 40.0 24.0 41.0 50-51 35.863388060439384 38.0 34.5 40.0 28.0 41.0 52-53 35.94297062152967 38.0 35.0 40.0 28.5 41.0 54-55 35.76633364737778 38.0 34.5 40.0 28.0 41.0 56-57 35.54411669947548 38.0 34.0 40.0 28.0 41.0 58-59 35.3038813402568 38.0 34.0 40.0 27.0 41.0 60-61 35.044628117869735 37.0 34.0 40.0 27.0 41.0 62-63 34.72229428341247 37.0 33.5 39.5 26.0 41.0 64-65 34.38603999357931 36.0 33.0 39.0 26.0 40.5 66-67 34.04482949521708 36.0 33.0 39.0 26.0 40.0 68-69 33.683141926357024 35.0 32.0 38.5 25.5 40.0 70-71 33.32450787100106 35.0 32.0 38.0 25.0 40.0 72-73 32.94636436044169 35.0 31.5 37.0 25.0 39.5 74-75 32.58217816195162 35.0 31.0 37.0 24.0 39.0 76-77 31.69215923413316 34.0 30.5 36.0 23.5 38.0 78-79 32.15001398647969 34.0 31.0 36.0 24.0 38.0 80-81 32.02834425880109 34.0 31.0 35.5 24.0 37.0 82-83 31.757686749370546 34.0 31.0 35.0 24.0 37.0 84-85 31.4715250270293 34.0 31.0 35.0 23.5 36.0 86-87 31.204114211013213 34.0 30.5 35.0 23.0 36.0 88-89 30.964007278307307 34.0 30.0 35.0 21.5 36.0 90-91 30.736685360635754 34.0 30.0 35.0 20.0 35.0 92-93 30.534091234042165 34.0 29.5 35.0 20.0 35.0 94-95 30.324286286018364 34.0 29.0 35.0 20.0 35.0 96-97 30.120802896376897 34.0 29.0 35.0 19.0 35.0 98-99 29.88603357543097 33.0 29.0 35.0 18.5 35.0 100 27.408766148474268 31.0 24.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2937557037948757 1101 2 -0.3167473714988098 1101 3 -0.3128917251232366 1101 4 -0.20859481142118597 1101 5 -0.20985466468923164 1101 6 -0.28990134578771887 1101 7 -0.31756605205946187 1101 8 -0.32357356340369847 1101 9 -0.45282181432222757 1101 10-11 -0.42905894640048103 1101 12-13 -0.46446353736583745 1101 14-15 -0.5323843169716795 1101 16-17 -0.6037063102296756 1101 18-19 -0.6243977860313166 1101 20-21 -0.6770462732875231 1101 22-23 -0.6875381724141221 1101 24-25 -0.6561718007919026 1101 26-27 -0.6720570622256972 1101 28-29 -0.7100544672688045 1101 30-31 -0.6683336986071353 1101 32-33 -0.6843528647066748 1101 34-35 -0.7178120294759864 1101 36-37 -0.8177235719983074 1101 38-39 -0.7275571666786291 1101 40-41 -0.7743900743458312 1101 42-43 -0.7265139485356471 1101 44-45 -0.76327065168487 1101 46-47 -0.7953946145603155 1101 48-49 0.33542035217802635 1101 50-51 -0.4664104633999173 1101 52-53 -0.7947127407354202 1101 54-55 -0.8360102143170067 1101 56-57 -0.8427030852589965 1101 58-59 -0.897601294473084 1101 60-61 -1.0011648238960689 1101 62-63 -0.9774295781311793 1101 64-65 -1.0107514748664812 1101 66-67 -1.000307089035168 1101 68-69 -0.9465966894432611 1101 70-71 -0.9374659602963149 1101 72-73 -1.108829895561648 1101 74-75 -1.1934448876277202 1101 76-77 -1.0061533273309209 1101 78-79 -0.8762185417164794 1101 80-81 -0.8193303687757876 1101 82-83 -0.7989939888164521 1101 84-85 -0.9285263586851009 1101 86-87 -0.948375601394833 1101 88-89 -0.9829207488598612 1101 90-91 -0.979719626830299 1101 92-93 -1.0882022243856682 1101 94-95 -1.0336797531068846 1101 96-97 -1.2007107187696668 1101 98-99 -1.3953936971992533 1101 100 -1.1187492188855401 1102 1 -0.32412124106313556 1102 2 -0.3290151222227138 1102 3 -0.33423860870384203 1102 4 -0.2421758310133555 1102 5 -0.1670690082099 1102 6 -0.30413488410901834 1102 7 -0.2420630770501191 1102 8 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0.5092551251782425 2311 10-11 0.41310436488323177 2311 12-13 0.49603168282106935 2311 14-15 0.48835837392782366 2311 16-17 0.5165676808588202 2311 18-19 0.7037610910404624 2311 20-21 0.7291327969027677 2311 22-23 0.7476475747528042 2311 24-25 0.7179349896534148 2311 26-27 0.7683528695473782 2311 28-29 0.8415495213045006 2311 30-31 0.6701199594751515 2311 32-33 0.7184387121279698 2311 34-35 0.8034243705952164 2311 36-37 0.8213463553779263 2311 38-39 0.823146912555579 2311 40-41 0.8047836537563242 2311 42-43 0.9090479052044174 2311 44-45 0.896186957211178 2311 46-47 0.9713209198797443 2311 48-49 -5.62579522946146 2311 50-51 -0.8604744860961873 2311 52-53 0.5148032022172586 2311 54-55 0.9220871641191266 2311 56-57 0.9946581911909007 2311 58-59 0.983444746191914 2311 60-61 0.815573930127762 2311 62-63 0.7629265969793195 2311 64-65 0.8528483905676438 2311 66-67 0.9690230877441053 2311 68-69 0.9817214936132146 2311 70-71 1.0248763730279364 2311 72-73 0.5170675904511697 2311 74-75 0.8101030521014607 2311 76-77 0.8252198148647949 2311 78-79 0.7510025397649329 2311 80-81 0.8454752202698899 2311 82-83 0.8581278186491623 2311 84-85 0.9408141607873581 2311 86-87 0.9287234109650804 2311 88-89 0.9784980754119239 2311 90-91 1.0920157725497113 2311 92-93 1.0243392810162888 2311 94-95 1.0304092757823327 2311 96-97 1.1241537967012967 2311 98-99 1.1677873604353657 2311 100 1.4205162693370958 2312 1 0.13346130456470817 2312 2 0.2779541386473028 2312 3 0.24614158114079032 2312 4 0.06965522490121145 2312 5 0.25561553764109846 2312 6 0.1699372395102614 2312 7 0.35983694430640867 2312 8 0.3831004396270785 2312 9 0.3333693770351971 2312 10-11 0.29719514449352147 2312 12-13 0.3684985729003074 2312 14-15 0.3597661643335712 2312 16-17 0.503276995947779 2312 18-19 0.3565172126251852 2312 20-21 0.4590594049858865 2312 22-23 0.7236996162938709 2312 24-25 0.7781729848146313 2312 26-27 0.6491986806882082 2312 28-29 0.8257286066971403 2312 30-31 0.8613731926062158 2312 32-33 0.8504247123381319 2312 34-35 0.44576819596372985 2312 36-37 0.7658996610526643 2312 38-39 0.9068970977128714 2312 40-41 0.6916654341528243 2312 42-43 0.6861924063811955 2312 44-45 0.8658899396044575 2312 46-47 0.8657763928758939 2312 48-49 -4.309819130893214 2312 50-51 -0.6860847455189472 2312 52-53 0.5845818617164937 2312 54-55 0.8808007292799331 2312 56-57 0.9684324183095683 2312 58-59 1.039460996987927 2312 60-61 1.030274405776261 2312 62-63 0.7600550448611898 2312 64-65 1.0178698055111255 2312 66-67 1.032871491719952 2312 68-69 0.9575524541902283 2312 70-71 1.0050979667352493 2312 72-73 1.0468842752432082 2312 74-75 0.7525550283152427 2312 76-77 0.9348780619918529 2312 78-79 0.617738796030217 2312 80-81 0.7907603561595167 2312 82-83 0.7859040385033431 2312 84-85 0.8981845601826954 2312 86-87 0.9575308144712302 2312 88-89 1.0039139038594236 2312 90-91 0.9140653782438157 2312 92-93 1.0383771467889673 2312 94-95 1.1774133992509057 2312 96-97 1.2127281995453103 2312 98-99 1.1666020372995014 2312 100 0.8132478488774986 2313 1 0.10640860361443671 2313 2 0.2693001158172095 2313 3 0.31790786210895305 2313 4 0.12925759028702544 2313 5 0.20326375443893596 2313 6 0.3154338387976878 2313 7 0.1829137010722519 2313 8 0.28482574468387867 2313 9 0.4883398462541777 2313 10-11 0.2913254446150759 2313 12-13 0.06863017134453742 2313 14-15 0.21831238642958084 2313 16-17 0.14645095090551763 2313 18-19 0.4546835434847907 2313 20-21 0.46458270886689235 2313 22-23 0.5009114345503747 2313 24-25 0.2583669439174088 2313 26-27 0.42013502298593863 2313 28-29 0.41857463438579856 2313 30-31 0.5530632819242101 2313 32-33 0.4737199329966515 2313 34-35 0.3657987788380197 2313 36-37 -0.32549367401720275 2313 38-39 0.16099996606320843 2313 40-41 0.3591175054427822 2313 42-43 0.4643473387009891 2313 44-45 0.5088718380581767 2313 46-47 0.4195309102537692 2313 48-49 -5.231868350343547 2313 50-51 -1.3138128142161634 2313 52-53 0.16795828874930407 2313 54-55 0.36750334840444765 2313 56-57 0.4268566036772583 2313 58-59 0.4192838397079939 2313 60-61 0.7384578764684093 2313 62-63 0.867293154182299 2313 64-65 0.9348105107528397 2313 66-67 0.9052678166835832 2313 68-69 0.9405661450448477 2313 70-71 0.9940670901274871 2313 72-73 0.7927676368038945 2313 74-75 0.9454831040528049 2313 76-77 0.8872284078914987 2313 78-79 0.8225449042248627 2313 80-81 -0.11219123591795466 2313 82-83 0.3393717104940954 2313 84-85 0.6800564664423128 2313 86-87 0.8411627890197728 2313 88-89 0.9180634346014678 2313 90-91 0.9699221880765769 2313 92-93 0.9848453138573561 2313 94-95 0.89701817516357 2313 96-97 0.9366527150967094 2313 98-99 1.0449491540188838 2313 100 1.2021222260184956 2314 1 0.054104920079101504 2314 2 0.17557824529608013 2314 3 0.22408482378576622 2314 4 0.04750600022802587 2314 5 0.21501870269393208 2314 6 0.2947043855183793 2314 7 0.14902595859985013 2314 8 0.2679812121414784 2314 9 0.4297387942936979 2314 10-11 0.31514048663799343 2314 12-13 0.2193019876112814 2314 14-15 0.3187764609962329 2314 16-17 0.5245036843652144 2314 18-19 0.5767501520337319 2314 20-21 0.584763273057149 2314 22-23 0.3695371895235766 2314 24-25 0.5010552414663181 2314 26-27 0.557201540546977 2314 28-29 0.7021199099400235 2314 30-31 0.6418692165658584 2314 32-33 0.5306565724180174 2314 34-35 0.4346881205821589 2314 36-37 0.5480595232704459 2314 38-39 0.5954295324749097 2314 40-41 0.3920088514220623 2314 42-43 0.40160342083363787 2314 44-45 0.5851269870417397 2314 46-47 0.6752502424869036 2314 48-49 -6.001929509996543 2314 50-51 -1.3257682981859986 2314 52-53 -0.6870635524979107 2314 54-55 -0.28598722302171353 2314 56-57 0.07464274747282218 2314 58-59 0.5071575894905962 2314 60-61 0.7021632846646568 2314 62-63 0.5906258340623296 2314 64-65 0.805018152173389 2314 66-67 0.852447658428666 2314 68-69 0.8182117996522393 2314 70-71 0.6527599434532618 2314 72-73 0.9254013368155825 2314 74-75 0.945319904352715 2314 76-77 0.7268616984517422 2314 78-79 0.5932605515353515 2314 80-81 0.6537109932320533 2314 82-83 0.6578506687191243 2314 84-85 0.682341180430388 2314 86-87 0.7361608312895633 2314 88-89 0.6504120286465991 2314 90-91 0.8355968200597026 2314 92-93 0.8538245191639362 2314 94-95 0.9262263399428221 2314 96-97 0.9371266352914169 2314 98-99 1.0031012142510285 2314 100 -0.281457144581033 2315 1 0.11980885818972453 2315 2 0.2017628469679167 2315 3 0.27362598338640254 2315 4 0.12122717151196838 2315 5 0.2215891610601446 2315 6 0.2949006801231988 2315 7 0.3813074420584144 2315 8 0.2737498603343411 2315 9 0.4382694052307272 2315 10-11 0.21087373748157745 2315 12-13 0.41217249925309574 2315 14-15 0.26363574074621 2315 16-17 0.34757134626102015 2315 18-19 0.43130040980245354 2315 20-21 0.36372518399287657 2315 22-23 0.38784034684553603 2315 24-25 0.41734856222664973 2315 26-27 0.49542638190856536 2315 28-29 0.6257649061471184 2315 30-31 0.5825161084642687 2315 32-33 0.6615548209429036 2315 34-35 0.644905510636228 2315 36-37 0.5716482156495459 2315 38-39 0.5989795668828037 2315 40-41 0.693790955020873 2315 42-43 0.7145111761020075 2315 44-45 0.7143284210580987 2315 46-47 0.5988402377063053 2315 48-49 -4.622687576426607 2315 50-51 -0.8510470913380317 2315 52-53 0.23534705534516576 2315 54-55 0.247761287955079 2315 56-57 0.571397841783785 2315 58-59 0.682427221679994 2315 60-61 0.5253974049487411 2315 62-63 0.2780381960448821 2315 64-65 0.45332213580125824 2315 66-67 -0.2582354794257995 2315 68-69 0.4699394656537592 2315 70-71 0.5050377836539113 2315 72-73 0.5756536535531964 2315 74-75 0.7944093950442763 2315 76-77 0.728723363338446 2315 78-79 0.6073188967145526 2315 80-81 0.5460249690581378 2315 82-83 0.4818192036000859 2315 84-85 0.464437050227108 2315 86-87 0.5680583583226202 2315 88-89 0.6049290346444778 2315 90-91 0.685631104712936 2315 92-93 0.7133711988167519 2315 94-95 0.45152644803161834 2315 96-97 0.4705573823459126 2315 98-99 0.3834742479451805 2315 100 0.444978705734119 2316 1 0.22014218780171646 2316 2 0.24059972543693675 2316 3 0.22244027962338464 2316 4 -0.0064573575331721145 2316 5 0.1977043473083384 2316 6 0.16211043359515998 2316 7 0.26463241028248063 2316 8 0.325942203848399 2316 9 0.48557261356090464 2316 10-11 0.4683132016838627 2316 12-13 0.3896013718731055 2316 14-15 0.5389441947481259 2316 16-17 0.5272374917018041 2316 18-19 0.2165436207027298 2316 20-21 0.45802077726281 2316 22-23 0.6377195971068801 2316 24-25 0.6767922321825921 2316 26-27 0.6635359566846901 2316 28-29 0.7159282621228513 2316 30-31 0.6577643004248799 2316 32-33 0.6172198685076395 2316 34-35 0.598265968618243 2316 36-37 0.5135486006187691 2316 38-39 0.5435163983441029 2316 40-41 0.26626945653170253 2316 42-43 0.1690582404712302 2316 44-45 0.4447483170805455 2316 46-47 0.49206746835789517 2316 48-49 -4.682543674377484 2316 50-51 -0.9177447956084208 2316 52-53 0.33422502221743144 2316 54-55 0.6381431264912791 2316 56-57 0.5159815503197862 2316 58-59 0.41410835685818626 2316 60-61 0.5467795031081693 2316 62-63 0.49086928394218887 2316 64-65 0.47021225342032125 2316 66-67 0.6378388416998533 2316 68-69 0.29970258381177217 2316 70-71 -0.100797349187431 2316 72-73 0.07575092318628407 2316 74-75 0.29992982083585673 2316 76-77 0.2672283967747404 2316 78-79 0.2919197479841742 2316 80-81 0.2573242324455407 2316 82-83 0.19067519394866395 2316 84-85 0.38497603801009106 2316 86-87 0.4151343666429135 2316 88-89 0.21414873503500687 2316 90-91 0.34334893346469997 2316 92-93 0.3985497295678506 2316 94-95 0.5413772703816413 2316 96-97 0.6365057066359832 2316 98-99 0.6494468837052025 2316 100 -0.04519321329479098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 11.0 11 19.0 12 43.0 13 203.0 14 800.0 15 2358.0 16 5350.0 17 10924.0 18 18378.0 19 26463.0 20 37207.0 21 51936.0 22 63130.0 23 67188.0 24 70642.0 25 78249.0 26 93700.0 27 115694.0 28 141102.0 29 173566.0 30 211827.0 31 263960.0 32 330862.0 33 427706.0 34 583692.0 35 814086.0 36 1220523.0 37 1691057.0 38 1236529.0 39 131091.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.368774975801266 20.34876433268066 14.712868387109568 23.569592304408506 2 15.351749740886216 20.67446475766586 40.16945501269991 23.80433048874801 3 18.587774801844258 25.871540837379737 30.18935715006508 25.351327210710924 4 12.866607768236795 17.28204508907858 38.113364646959 31.737982495725625 5 13.395521434865234 37.62024707054745 34.66671682587768 14.317514668709638 6 30.76179001589922 37.39060865675094 17.74655548650218 14.101045840847654 7 27.339501450819625 32.404222456382314 22.182422631743968 18.073853461054092 8 25.873600779050538 35.510131558829336 19.999252672743488 18.61701498937664 9 26.104885913414005 16.195888518154955 20.69311809722358 37.006107471207464 10-11 25.035069847061468 26.39880155966532 27.789883781307008 20.776244811966205 12-13 25.462304084259053 23.99307423986815 28.202671578863352 22.341950097009445 14-15 22.870125506081106 25.44227447236406 25.917186219546167 25.77041380200867 16-17 22.108401004793294 28.308494163594734 26.78234202243564 22.800762809176334 18-19 21.874726354868283 27.359041857903176 29.000391570694145 21.7658402165344 20-21 22.860696353015157 26.516596706687128 28.86632043382135 21.75638650647636 22-23 22.76195072695205 26.36304740637984 28.64779197787069 22.227209888797418 24-25 22.433442196171452 26.531863064385735 28.887362230598157 22.147332508844656 26-27 22.33454765624325 26.728643566062743 28.656453204424636 22.28035557326937 28-29 22.098062057572708 26.743540679099603 28.89871714672731 22.25968011660038 30-31 22.529541035390523 26.573262771819913 28.796327917090263 22.100868275699302 32-33 22.46438691849253 26.813094167017947 28.510128178558602 22.212390735930917 34-35 22.59356452574362 26.75466436283977 28.69128230199136 21.96048880942525 36-37 22.600611363292856 26.58783926791998 28.493954438981316 22.317594929805846 38-39 22.50288287368688 26.72576290247653 28.659663787299312 22.111690436537277 40-41 22.471366305329862 26.424811922328782 28.90738697879673 22.19643479354463 42-43 22.38048662565242 26.52247898157028 28.5917688628241 22.505265529953196 44-45 22.359462068328252 26.851430353408425 28.43603242059402 22.35307515766931 46-47 22.43402151845599 26.6964158891735 28.124569228906637 22.744993363463877 48-49 22.778628742955952 26.777752729422122 27.964384454168638 22.479234073453284 50-51 22.572801619329244 26.859247173082462 27.815969851636485 22.75198135595181 52-53 22.434915515425722 27.05700377003193 27.755893213211618 22.75218750133073 54-55 22.478036796778074 26.9859605055728 27.73318414747284 22.802818550176287 56-57 22.625852230227768 26.950194967067414 27.562575474351654 22.861377328353164 58-59 22.37150128132028 26.90667727887788 27.897396762902748 22.824424676899095 60-61 22.483817217489225 27.138116378767986 27.676294781473654 22.701771622269135 62-63 22.496596701375726 26.967286453298595 27.65102203181869 22.88509481350699 64-65 22.214232988429785 26.84943135341434 27.73427871964298 23.2020569385129 66-67 22.366497840018546 26.821965317110752 27.56823202897527 23.24330481389543 68-69 22.184580477361198 26.86141666557549 27.6631907043359 23.290812152727412 70-71 22.200598448427492 26.60282250631869 27.77819728661683 23.41838175863699 72-73 22.31619965841423 26.803220041122245 27.580282857322825 23.300297443140703 74-75 22.26877239560243 26.94813142888519 27.47126321365964 23.311832961852737 76-77 22.149064144146134 26.86084858159064 27.47344818090224 23.516639093360983 78-79 22.062513508568554 26.787056914979445 27.522139117935673 23.628290458516325 80-81 21.92391367550181 27.015852932775086 27.47982121483547 23.58041217688763 82-83 22.200554323654593 26.732363108051167 27.70366881926265 23.363413749031587 84-85 22.20177969872243 26.769923091590925 27.399580499586857 23.62871671009979 86-87 21.780090549103384 26.97174959755283 27.651824453647816 23.596335399695967 88-89 21.925922243826772 26.642239234791614 27.590581528444964 23.84125699293665 90-91 21.987939133811672 26.571309807779947 27.63888332751773 23.801867730890653 92-93 21.915018629296853 26.68845936906663 27.54846079903398 23.84806120260254 94-95 21.51941368895837 26.502822408317407 27.774417049157407 24.203346853566813 96-97 21.636529161997995 26.43443456369356 27.714625025165073 24.21441124914337 98-99 21.454815369584963 26.545889335388466 27.596457764351435 24.402837530675132 100 21.46674523549353 26.411508680393325 27.590155503981517 24.53159058013162 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2154.0 1 1521.0 2 1858.5 3 2577.5 4 1736.5 5 1266.5 6 1852.5 7 3496.0 8 5577.0 9 6589.0 10 5918.0 11 4921.0 12 5031.0 13 5574.5 14 6237.5 15 7064.0 16 7880.0 17 8728.5 18 9547.5 19 10429.5 20 11562.0 21 12995.5 22 14831.5 23 17542.0 24 20925.0 25 25047.0 26 31219.5 27 38628.5 28 46698.5 29 58282.0 30 73883.0 31 90351.5 32 110919.5 33 134478.5 34 156637.5 35 178539.5 36 203259.0 37 229529.5 38 242742.5 39 245230.0 40 244820.0 41 243579.0 42 247216.5 43 257124.0 44 270414.0 45 287655.0 46 301948.0 47 308935.5 48 316188.5 49 318011.0 50 310568.0 51 301906.5 52 292406.0 53 282431.0 54 272160.5 55 251678.5 56 226204.0 57 198846.0 58 173294.0 59 152464.0 60 126945.5 61 101401.5 62 81689.0 63 63912.5 64 47598.5 65 34875.0 66 25762.0 67 19827.5 68 15576.0 69 11994.5 70 9336.5 71 7294.0 72 5820.5 73 4687.0 74 3626.0 75 2707.5 76 1902.5 77 1214.0 78 763.0 79 494.5 80 317.5 81 192.5 82 119.5 83 73.5 84 46.0 85 33.5 86 20.0 87 11.5 88 9.5 89 6.5 90 6.0 91 5.5 92 3.5 93 2.0 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027947540978606213 2 0.0018809622850539881 3 8.896443240120214E-5 4 0.02113540729759988 5 4.321129573772675E-4 6 3.812761388622949E-5 7 2.5418409257486325E-5 8 0.0034823220682756264 9 0.009353974606754967 10-11 0.0010993462003862837 12-13 8.896443240120214E-5 14-15 1.1438284165868847E-4 16-17 3.5585772960480854E-4 18-19 2.5418409257486325E-5 20-21 6.35460231437158E-5 22-23 1.9699267174551902E-4 24-25 2.5418409257486325E-5 26-27 6.35460231437158E-5 28-29 9.023535286407645E-4 30-31 0.002414748879461201 32-33 0.00932220159518311 34-35 0.017824659491812285 36-37 0.02074142195410884 38-39 0.02029024518978846 40-41 0.004492703836260708 42-43 0.0050391996352966645 44-45 0.008381720452656116 46-47 0.011190454675608355 48-49 1.2005178238333936 50-51 0.017182844658060754 52-53 0.01783736869644103 54-55 0.009176045741952564 56-57 0.0072950834568985745 58-59 0.008222855394796827 60-61 0.004867625372808631 62-63 0.00735227487772792 64-65 0.01875878603202491 66-67 0.027420108986513373 68-69 0.0278839949554625 70-71 0.025049842323252774 72-73 0.02246351918130354 74-75 0.008858315626233985 76-77 0.014011898103189337 78-79 0.007898770676763875 80-81 0.0026752875743504354 82-83 0.013573430543497696 84-85 0.023041787991911356 86-87 0.02227288111187239 88-89 0.030877012645531512 90-91 0.019673848765294415 92-93 0.012715559231057534 94-95 0.006043226800967374 96-97 0.0033679392266169382 98-99 0.015136662712833107 100 0.03301851362547474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7868313.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.19013853377974 #Duplication Level Percentage of deduplicated Percentage of total 1 74.71940641491508 44.226520168606136 2 14.995945771623328 17.752242153348664 3 4.863671624113778 8.63644191642324 4 2.1053718439183573 4.984690044265875 5 1.0184649607277587 3.0141541058638297 6 0.5658604818409745 2.009601618657518 7 0.3812323385605392 1.5795636453068596 8 0.25204060865305583 1.193465483385003 9 0.18796652366341549 1.0013188117815495 >10 0.837752112468448 8.569257999503764 >50 0.0442493389882965 1.799939844346465 >100 0.025579146657108094 2.856064054176361 >500 0.0018591234136906317 0.7523467066511702 >1k 4.797683650024734E-4 0.501571832606942 >5k 2.9985522806222388E-5 0.09486558545413874 >10k+ 8.995656841866716E-5 1.027956029622602 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24572 0.312290576137477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20811 0.26449125752877395 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12535 0.15930988002129554 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.5418409257486325E-5 0.0 1.2709204628743163E-5 0.0 5 0.0 2.5418409257486325E-5 0.0 1.2709204628743163E-5 0.0 6 0.0 2.5418409257486325E-5 0.0 1.2709204628743163E-5 1.2709204628743163E-5 7 0.0 2.5418409257486325E-5 0.0 2.5418409257486325E-5 1.2709204628743163E-5 8 0.0 2.5418409257486325E-5 0.0 2.5418409257486325E-5 1.2709204628743163E-5 9 0.0 2.5418409257486325E-5 0.0 3.8127613886229485E-5 1.2709204628743163E-5 10-11 0.0 3.17730115718579E-5 0.0 3.8127613886229485E-5 1.2709204628743163E-5 12-13 0.0 3.8127613886229485E-5 0.0 3.8127613886229485E-5 1.2709204628743163E-5 14-15 0.0 3.8127613886229485E-5 0.0 3.8127613886229485E-5 1.9063806943114742E-5 16-17 0.0 3.8127613886229485E-5 0.0 4.448221620060107E-5 3.8127613886229485E-5 18-19 0.0 3.8127613886229485E-5 0.0 8.260983008683055E-5 6.354602314371582E-5 20-21 0.0 4.448221620060107E-5 0.0 1.016736370299453E-4 6.354602314371582E-5 22-23 0.0 6.354602314371582E-5 0.0 2.541840925748632E-4 6.354602314371582E-5 24-25 0.0 7.625522777245897E-5 0.0 8.388075054970487E-4 7.625522777245897E-5 26-27 0.0 9.531903471557372E-5 0.0 0.0017030334202515838 7.625522777245897E-5 28-29 0.0 1.0802823934431688E-4 0.0 0.004086009288140927 8.896443240120214E-5 30-31 0.0 1.398012509161748E-4 0.0 0.012760041447258135 1.016736370299453E-4 32-33 0.0 1.5251045554491794E-4 0.0 0.03404795920040293 1.1438284165868847E-4 34-35 0.0 1.652196601736611E-4 0.0 0.06838823010726697 1.1438284165868847E-4 36-37 0.0 1.652196601736611E-4 0.0 0.12390839052793146 1.2073744397306005E-4 38-39 6.354602314371581E-6 1.652196601736611E-4 0.0 0.20189842473221387 1.3980125091617477E-4 40-41 1.2709204628743163E-5 1.652196601736611E-4 0.0 0.2851310058458528 1.5251045554491794E-4 42-43 1.2709204628743163E-5 1.715742624880327E-4 0.0 0.3696980534455099 1.5251045554491794E-4 44-45 1.9063806943114742E-5 1.7792886480240427E-4 0.0 0.4743710627678386 1.5251045554491794E-4 46-47 2.5418409257486325E-5 1.7792886480240427E-4 0.0 0.6016601017270157 1.5251045554491794E-4 48-49 2.5418409257486325E-5 1.9063806943114744E-4 0.0 0.7373117973319059 1.5251045554491794E-4 50-51 2.5418409257486325E-5 1.9063806943114744E-4 0.0 0.881770971744515 1.5251045554491794E-4 52-53 2.5418409257486325E-5 1.9063806943114744E-4 0.0 1.023834461084606 1.5886505785928952E-4 54-55 2.5418409257486325E-5 1.9063806943114744E-4 0.0 1.184447034580348 1.652196601736611E-4 56-57 3.8127613886229485E-5 2.0970187637426219E-4 0.0 1.372397107232516 1.652196601736611E-4 58-59 3.8127613886229485E-5 2.2241108100300535E-4 0.0 1.5805865882559578 1.652196601736611E-4 60-61 3.8127613886229485E-5 2.2876568331737693E-4 0.0 1.7955945575627204 1.652196601736611E-4 62-63 3.8127613886229485E-5 2.2876568331737693E-4 0.0 2.0154701522422913 1.7792886480240427E-4 64-65 3.8127613886229485E-5 2.2876568331737693E-4 0.0 2.2402197268969855 1.7792886480240427E-4 66-67 3.8127613886229485E-5 2.2876568331737693E-4 0.0 2.4930820621904592 1.7792886480240427E-4 68-69 3.8127613886229485E-5 2.2876568331737693E-4 0.0 2.775588617280477 1.8428346711677585E-4 70-71 3.8127613886229485E-5 2.2876568331737693E-4 0.0 3.075030187538294 2.033472740598906E-4 72-73 3.8127613886229485E-5 2.2876568331737693E-4 0.0 3.3762701102510793 2.5418409257486327E-4 74-75 3.8127613886229485E-5 2.2876568331737693E-4 0.0 3.6735701795289537 2.5418409257486327E-4 76-77 3.8127613886229485E-5 2.2876568331737693E-4 0.0 4.001200765653324 2.73247899517978E-4 78-79 3.8127613886229485E-5 2.2876568331737693E-4 0.0 4.360305697040776 2.9866630877546435E-4 80-81 3.8127613886229485E-5 2.2876568331737693E-4 0.0 4.740730319192945 3.1773011571857904E-4 82-83 3.8127613886229485E-5 2.2876568331737693E-4 0.0 5.123448952780603 3.304393203473222E-4 84-85 3.8127613886229485E-5 2.2876568331737693E-4 0.0 5.499673182802972 3.304393203473222E-4 86-87 3.8127613886229485E-5 2.414748879461201E-4 0.0 5.8928514918000845 3.367939226616938E-4 88 3.8127613886229485E-5 2.5418409257486327E-4 0.0 6.206107967489346 3.5585772960480854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13120 0.0 53.616833 1 GTATCAA 23835 0.0 40.91323 1 CAACGCA 29925 0.0 31.933714 5 ATCAACG 29870 0.0 31.919666 3 TATCAAC 31480 0.0 30.761992 2 TCAACGC 31280 0.0 30.555641 4 AACGCAG 32260 0.0 30.02932 6 GTGGTAT 5905 0.0 27.338326 1 GTACTAG 3075 0.0 27.16491 1 ACGCAGA 36270 0.0 26.347126 7 CGCAGAG 37335 0.0 25.620693 8 TGGTATC 6570 0.0 23.780355 2 GTACATG 31160 0.0 23.208044 1 GCAGAGT 40940 0.0 23.088606 9 TACATGG 32215 0.0 22.181007 2 ACATGGG 32240 0.0 21.524994 3 GAGTACT 23635 0.0 20.759468 12-13 CAGAGTA 40240 0.0 20.21283 10-11 AGAGTAC 38560 0.0 19.974325 10-11 GCATCGT 3425 0.0 19.860823 8 >>END_MODULE