FastQCFastQC Report
Mon 6 Jun 2016
ERR1211277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9240607
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348790.37745355905732164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276660.2993959163072296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155680.1684737809972873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172850.051.9444471
GTATCAA318100.041.724961
CAACGCA385050.033.458645
ATCAACG386900.033.2743643
TATCAAC400700.032.746782
TCAACGC408300.031.6564434
AACGCAG411600.031.6432116
ACGCAGA461100.027.9315747
CGCAGAG471600.027.278918
GTGGTAT76050.026.6551191
GTACTAG38450.026.0546881
GTACATG436600.025.6279161
TACATGG446800.024.8101962
GCAGAGT510800.024.6719519
ACATGGG453900.023.7563923
GAGTACT268500.023.05745312-13
TGGTATC88600.021.8699252
GTACTTT287850.021.32986614-15
CATGGGG309250.021.133354
CAGAGTA505950.020.92948210-11
Produced by FastQC (version 0.11.5)