##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211277_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9240607 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.496835976251344 31.0 31.0 34.0 30.0 34.0 2 31.521446805388432 31.0 31.0 34.0 28.0 34.0 3 31.57122405486999 33.0 31.0 34.0 28.0 34.0 4 35.18403163341975 37.0 35.0 37.0 32.0 37.0 5 35.22423126532705 37.0 35.0 37.0 32.0 37.0 6 35.28882713007923 37.0 35.0 37.0 32.0 37.0 7 35.22533368208387 37.0 35.0 37.0 32.0 37.0 8 35.22797972037984 37.0 35.0 37.0 32.0 37.0 9 36.79531398749022 39.0 37.0 39.0 32.0 39.0 10-11 36.73469940881589 39.0 37.0 39.0 32.0 39.0 12-13 36.64765913105059 39.0 37.0 39.0 32.0 39.0 14-15 37.789905576549245 40.0 37.5 41.0 32.0 41.0 16-17 37.789342788844934 40.0 37.5 41.0 32.0 41.0 18-19 37.79378513770794 40.0 37.5 41.0 32.0 41.0 20-21 37.75855980023823 40.0 37.5 41.0 32.0 41.0 22-23 37.63642512878212 40.0 37.0 41.0 32.0 41.0 24-25 37.520047222006085 40.0 37.0 41.0 32.0 41.0 26-27 37.37235687006276 40.0 37.0 41.0 31.0 41.0 28-29 37.23916004652075 40.0 36.5 41.0 31.0 41.0 30-31 37.0525105114848 39.0 36.0 41.0 30.5 41.0 32-33 36.92778840177924 39.0 36.0 41.0 30.0 41.0 34-35 36.7804225415062 39.0 35.5 41.0 30.0 41.0 36-37 36.68410354428015 39.0 35.0 41.0 30.0 41.0 38-39 36.525486042204804 39.0 35.0 41.0 30.0 41.0 40-41 36.35077457573945 39.0 35.0 40.0 29.0 41.0 42-43 36.19323871256509 39.0 35.0 40.0 28.5 41.0 44-45 36.038489192322544 39.0 35.0 40.0 28.0 41.0 46-47 35.84491478752424 38.5 35.0 40.0 27.0 41.0 48-49 35.58302219756776 38.0 34.0 40.0 26.0 41.0 50-51 34.89707640417994 37.5 33.5 39.5 25.5 40.5 52-53 35.12691947617727 38.0 34.0 40.0 26.0 40.5 54-55 35.46729457274831 38.0 34.0 40.0 26.0 41.0 56-57 35.34852867349515 38.0 34.0 40.0 26.0 41.0 58-59 35.104663308373574 38.0 34.0 40.0 26.0 41.0 60-61 34.827731230210304 37.0 34.0 40.0 26.0 41.0 62-63 34.546895187729554 37.0 33.0 40.0 26.0 41.0 64-65 34.258029856696645 36.0 33.0 39.5 25.0 41.0 66-67 34.028316375753235 36.0 33.0 39.0 25.0 41.0 68-69 33.686829555677456 35.0 33.0 39.0 24.0 41.0 70-71 33.34982301487337 35.0 32.0 38.5 24.0 40.0 72-73 32.99511563471967 35.0 32.0 37.5 23.0 40.0 74-75 32.62724223635958 35.0 32.0 37.0 22.5 39.0 76-77 32.282216904149266 35.0 31.0 36.5 22.0 39.0 78-79 31.929171211371717 35.0 31.0 36.0 21.0 39.0 80-81 31.607089880567372 35.0 31.0 35.5 20.0 37.5 82-83 31.30637392110713 34.0 30.5 35.0 20.0 37.0 84-85 31.016713566543842 34.0 30.0 35.0 19.5 36.5 86-87 30.76892167365196 34.0 30.0 35.0 19.0 36.0 88-89 30.522193726018216 34.0 29.5 35.0 18.0 36.0 90-91 30.268238763968647 34.0 29.0 35.0 18.0 35.5 92-93 30.03265321206713 34.0 29.0 35.0 17.5 35.0 94-95 29.817542992576136 34.0 29.0 35.0 17.0 35.0 96-97 29.618499412430374 34.0 29.0 35.0 15.0 35.0 98-99 29.38520878552675 34.0 27.5 35.0 14.0 35.0 100 27.67751880368898 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3246218025475436 1101 2 -0.30364377585746993 1101 3 -0.37517929140373596 1101 4 -0.3090008153154784 1101 5 -0.2907208948059079 1101 6 -0.25376910730256697 1101 7 -0.4092436040656864 1101 8 -0.3914285536543147 1101 9 -0.47684150411416226 1101 10-11 -0.4772405010530463 1101 12-13 -0.4339632497873751 1101 14-15 -0.5126942571884854 1101 16-17 -0.5258701769132514 1101 18-19 -0.5996768491310647 1101 20-21 -0.6336745799316716 1101 22-23 -0.5840842176746861 1101 24-25 -0.6297580336510364 1101 26-27 -0.6952056170244703 1101 28-29 -0.7407672939061527 1101 30-31 -0.7525735220102945 1101 32-33 -0.7528401798650677 1101 34-35 -0.7411009012768588 1101 36-37 -0.8926944396606373 1101 38-39 -0.877654916042161 1101 40-41 -0.8931956135859949 1101 42-43 -0.8535453330308229 1101 44-45 -0.9303768676819786 1101 46-47 -0.8594011841044491 1101 48-49 -0.8679767576578641 1101 50-51 -0.8131970226126839 1101 52-53 -0.6679082574475714 1101 54-55 -0.6551592020403731 1101 56-57 -0.6003020944039363 1101 58-59 -0.6801312973551887 1101 60-61 -0.6910550828229134 1101 62-63 -0.7085121385191542 1101 64-65 -0.7208100609296011 1101 66-67 -0.7676499725724071 1101 68-69 -0.799634662546346 1101 70-71 -0.7153385324516819 1101 72-73 -0.825605571194231 1101 74-75 -0.8496147418880255 1101 76-77 -0.8239314864118796 1101 78-79 -0.9409004338368874 1101 80-81 -0.9241028037817429 1101 82-83 -1.0046827508194234 1101 84-85 -0.9794368285440207 1101 86-87 -0.9820326974996156 1101 88-89 -0.9880836527673829 1101 90-91 -1.0970781257487339 1101 92-93 -1.0998246080947602 1101 94-95 -1.11232591112363 1101 96-97 -1.1805382669405446 1101 98-99 -1.2310497053467842 1101 100 -1.2919083006558765 1102 1 -0.34307388728687727 1102 2 -0.32983118712204984 1102 3 -0.35899525182815495 1102 4 -0.20160421585211452 1102 5 -0.2663404237238254 1102 6 -0.22003736606433222 1102 7 -0.346468808977491 1102 8 -0.25961614042850556 1102 9 -0.38881562856519025 1102 10-11 -0.3321017805592561 1102 12-13 -0.36277641661164495 1102 14-15 -0.46799935987621666 1102 16-17 -0.5357582148726721 1102 18-19 -0.48948633842297085 1102 20-21 -0.4479857942385408 1102 22-23 -0.5027085550366266 1102 24-25 -0.578033757198078 1102 26-27 -0.5193264066467691 1102 28-29 -0.6687518554442917 1102 30-31 -0.6789626584196782 1102 32-33 -0.6157655823820818 1102 34-35 -0.6305741278912578 1102 36-37 -0.6944104383790588 1102 38-39 -0.7835814047277978 1102 40-41 -0.7757658926343822 1102 42-43 -0.7643450877069071 1102 44-45 -0.813245422451196 1102 46-47 -0.8412793160502403 1102 48-49 -0.8136099699956034 1102 50-51 -0.7815411803012537 1102 52-53 -0.6387058233932521 1102 54-55 -0.6396063397242031 1102 56-57 -0.5829163360676901 1102 58-59 -0.6145873160202413 1102 60-61 -0.6844722752880301 1102 62-63 -0.7188945817579793 1102 64-65 -0.7556502882091465 1102 66-67 -0.6889830497828981 1102 68-69 -0.6417795969677584 1102 70-71 -0.7199882252178824 1102 72-73 -0.7040412503293325 1102 74-75 -0.7727956880796114 1102 76-77 -0.7735856285956579 1102 78-79 -0.7485758198254864 1102 80-81 -0.7620551400451774 1102 82-83 -0.8406408834447454 1102 84-85 -0.8826770571112057 1102 86-87 -1.023000882973676 1102 88-89 -1.017670954138275 1102 90-91 -1.0015773071186587 1102 92-93 -1.0819601816427564 1102 94-95 -1.062768865954574 1102 96-97 -1.1246901232252249 1102 98-99 -1.0843359091549978 1102 100 -1.1072518444936215 1103 1 -0.3990767757414133 1103 2 -0.4468116708038501 1103 3 -0.3642269818629664 1103 4 -0.32727114129163226 1103 5 -0.27553387672270446 1103 6 -0.29705704070409666 1103 7 -0.2766987441841948 1103 8 -0.3493260529982223 1103 9 -0.4577387865452991 1103 10-11 -0.42432405085283165 1103 12-13 -0.5212125615579737 1103 14-15 -0.5685382487047832 1103 16-17 -0.5916079682056861 1103 18-19 -0.6078300466752111 1103 20-21 -0.6926369156626535 1103 22-23 -0.7183175158378035 1103 24-25 -0.6975764133929871 1103 26-27 -0.6682672046295579 1103 28-29 -0.681672623533224 1103 30-31 -0.6531728551151161 1103 32-33 -0.7407377528521977 1103 34-35 -0.7998161974654394 1103 36-37 -0.8663264833002771 1103 38-39 -0.9410630708911043 1103 40-41 -0.8320624764560662 1103 42-43 -0.7936416472097747 1103 44-45 -0.8337214751234185 1103 46-47 -0.861045269080698 1103 48-49 -0.8486842972065105 1103 50-51 -0.8511247893216662 1103 52-53 -0.7833373720507097 1103 54-55 -0.8094964308770969 1103 56-57 -0.8231158614021297 1103 58-59 -0.889993620232076 1103 60-61 -0.7710908734297917 1103 62-63 -0.8594138551650303 1103 64-65 -0.8539661037801451 1103 66-67 -0.8696091835990742 1103 68-69 -0.8397871798949055 1103 70-71 -0.8478056206253868 1103 72-73 -0.9470943515374657 1103 74-75 -0.8667581591657054 1103 76-77 -0.798505549625439 1103 78-79 -0.8253554985268821 1103 80-81 -0.8355420847203732 1103 82-83 -0.996276321007219 1103 84-85 -0.9662748875407203 1103 86-87 -1.0194764831719496 1103 88-89 -1.0315018565689797 1103 90-91 -1.0189924300300355 1103 92-93 -1.1073541256379151 1103 94-95 -1.241488036458147 1103 96-97 -1.2380460374164883 1103 98-99 -1.360020079347244 1103 100 -1.4634303989789537 1104 1 -0.3512410069790519 1104 2 -0.35629933475438946 1104 3 -0.3941378708592964 1104 4 -0.2517317087042912 1104 5 -0.191578588381347 1104 6 -0.20819427329825402 1104 7 -0.258618427417332 1104 8 -0.2342945709718336 1104 9 -0.351272231879463 1104 10-11 -0.34964407352097737 1104 12-13 -0.4130457620883874 1104 14-15 -0.516820012840931 1104 16-17 -0.4573801436021867 1104 18-19 -0.5237915424474622 1104 20-21 -0.5081151015834209 1104 22-23 -0.4938684908186062 1104 24-25 -0.5831570677656899 1104 26-27 -0.5954273696363828 1104 28-29 -0.533588881026887 1104 30-31 -0.6320622648717205 1104 32-33 -0.7976922029939146 1104 34-35 -0.6424474758895329 1104 36-37 -0.6689390641196127 1104 38-39 -0.7038011563972901 1104 40-41 -0.8034458372532782 1104 42-43 -0.7482960249299637 1104 44-45 -0.7448996946467048 1104 46-47 -0.8199214557539207 1104 48-49 -0.8238812278274921 1104 50-51 -0.7471091725677397 1104 52-53 -0.6884668932872486 1104 54-55 -0.6491953937384238 1104 56-57 -0.6360081094188885 1104 58-59 -0.7340116089774966 1104 60-61 -0.6515547811078051 1104 62-63 -0.6471396347127083 1104 64-65 -0.6331655152959073 1104 66-67 -0.7439291520131732 1104 68-69 -0.5844380892514636 1104 70-71 -0.6966394324030176 1104 72-73 -0.6843751646187783 1104 74-75 -0.7911835193143517 1104 76-77 -0.6594672794070817 1104 78-79 -0.6754455018148668 1104 80-81 -0.8368929385356942 1104 82-83 -0.7716425090150523 1104 84-85 -0.8602343061902467 1104 86-87 -0.9456504425386569 1104 88-89 -0.8044833081769696 1104 90-91 -0.9062422149897991 1104 92-93 -0.9059724215368945 1104 94-95 -0.8933928150711417 1104 96-97 -0.9037883716751693 1104 98-99 -0.9718715035357626 1104 100 -1.0971356472931255 1105 1 -0.40101824065735414 1105 2 -0.4592006255651846 1105 3 -0.4008857067556377 1105 4 -0.26193633586182585 1105 5 -0.2353129684594606 1105 6 -0.25020635434374583 1105 7 -0.2912404137409581 1105 8 -0.30606699630036616 1105 9 -0.4262269735654556 1105 10-11 -0.4647367144597041 1105 12-13 -0.49511757120109223 1105 14-15 -0.5317748531503455 1105 16-17 -0.6018457759139793 1105 18-19 -0.599905722336743 1105 20-21 -0.6248886048982669 1105 22-23 -0.6148004398240516 1105 24-25 -0.5947763818527463 1105 26-27 -0.6041374477992179 1105 28-29 -0.6628696392933122 1105 30-31 -0.732876046853228 1105 32-33 -0.7425812679498591 1105 34-35 -0.7812268079023355 1105 36-37 -0.752404750889859 1105 38-39 -0.7134707447692747 1105 40-41 -0.839898952690298 1105 42-43 -0.7864972269985415 1105 44-45 -0.8126782824299426 1105 46-47 -0.7815361229533693 1105 48-49 -0.6932294288837824 1105 50-51 -0.727708686278838 1105 52-53 -0.5333906071293981 1105 54-55 -0.5190675990747522 1105 56-57 -0.6105273343972613 1105 58-59 -0.5696651054027058 1105 60-61 -0.5999785255744214 1105 62-63 -0.6428296026711422 1105 64-65 -0.6224559348849326 1105 66-67 -0.58363471691505 1105 68-69 -0.5582325062012643 1105 70-71 -0.5572710275578672 1105 72-73 -0.5980979356964937 1105 74-75 -0.6069286188419518 1105 76-77 -0.597715606493864 1105 78-79 -0.586696260723425 1105 80-81 -0.5843629379517665 1105 82-83 -0.6436157367448025 1105 84-85 -0.6248512400307185 1105 86-87 -0.7185423595437612 1105 88-89 -0.7215156292540783 1105 90-91 -0.6729082524606085 1105 92-93 -0.6993332856246361 1105 94-95 -0.7585077069083361 1105 96-97 -0.8103086555888197 1105 98-99 -0.8877492434782575 1105 100 -0.8804465222415665 1106 1 -0.41639507055920433 1106 2 -0.4486812375357978 1106 3 -0.452541909865424 1106 4 -0.35951832326333033 1106 5 -0.2717443433216147 1106 6 -0.30916646192592623 1106 7 -0.3625954981296573 1106 8 -0.38984796190344184 1106 9 -0.4385225733029685 1106 10-11 -0.45195448246207803 1106 12-13 -0.43087251956816885 1106 14-15 -0.5149294832627191 1106 16-17 -0.6574556451086337 1106 18-19 -0.6518813611652945 1106 20-21 -0.6297023095120338 1106 22-23 -0.6104726812922507 1106 24-25 -0.593879593206033 1106 26-27 -0.5566436672783084 1106 28-29 -0.6476983659301041 1106 30-31 -0.7432542207405319 1106 32-33 -0.703482764834277 1106 34-35 -0.721630468029332 1106 36-37 -0.7057562230784598 1106 38-39 -0.7033751839063882 1106 40-41 -0.7929971063828489 1106 42-43 -0.7440523320517585 1106 44-45 -0.6776571158136093 1106 46-47 -0.7291624084322379 1106 48-49 -0.7755608101722586 1106 50-51 -0.747370692080473 1106 52-53 -0.6276745188779174 1106 54-55 -0.70179596740779 1106 56-57 -0.633021335946701 1106 58-59 -0.6525628475731224 1106 60-61 -0.6794064760117706 1106 62-63 -0.6926429028356793 1106 64-65 -0.6516968555934923 1106 66-67 -0.6647867625110564 1106 68-69 -0.6265229144732984 1106 70-71 -0.6516836372952355 1106 72-73 -0.6651928501003894 1106 74-75 -0.658703451595926 1106 76-77 -0.6278929360389647 1106 78-79 -0.672086241493286 1106 80-81 -0.7517577253995107 1106 82-83 -0.7026758481933264 1106 84-85 -0.632768356836845 1106 86-87 -0.7255755667390815 1106 88-89 -0.758078209294446 1106 90-91 -0.7199788919931542 1106 92-93 -0.7347520001041943 1106 94-95 -0.8562016733043443 1106 96-97 -0.8736844052879249 1106 98-99 -0.8310136990414279 1106 100 -0.9328817750658835 1107 1 -0.43132024181221595 1107 2 -0.4156063841716744 1107 3 -0.40732746179842394 1107 4 -0.29680609241128764 1107 5 -0.30122785030612675 1107 6 -0.29412406492290444 1107 7 -0.33354418249738416 1107 8 -0.31314175663155197 1107 9 -0.4760321241512102 1107 10-11 -0.44394289447861013 1107 12-13 -0.4694640739893643 1107 14-15 -0.5445635628566166 1107 16-17 -0.5456201657359898 1107 18-19 -0.5082983939784711 1107 20-21 -0.5470125672200439 1107 22-23 -0.5931770868371515 1107 24-25 -0.6169952949677722 1107 26-27 -0.5766240819353072 1107 28-29 -0.5660444901420405 1107 30-31 -0.5630247207641474 1107 32-33 -0.5444092884245251 1107 34-35 -0.5636363461742278 1107 36-37 -0.5962720886244668 1107 38-39 -0.6362046752935413 1107 40-41 -0.640265266105807 1107 42-43 -0.6486172595576178 1107 44-45 -0.5888625034352373 1107 46-47 -0.6524834760080296 1107 48-49 -0.6717821393988075 1107 50-51 -0.5780520971893566 1107 52-53 -0.4886999271110355 1107 54-55 -0.3957233203548256 1107 56-57 -0.4352590473447222 1107 58-59 -0.47796036421209465 1107 60-61 -0.46012362279962815 1107 62-63 -0.5046114017266348 1107 64-65 -0.41829763796365427 1107 66-67 -0.46952819089651854 1107 68-69 -0.3761170193104775 1107 70-71 -0.5180531991650028 1107 72-73 -0.5233662623316562 1107 74-75 -0.49040668207376825 1107 76-77 -0.4481661675616948 1107 78-79 -0.42390045781131747 1107 80-81 -0.5088465786916601 1107 82-83 -0.47581154469181897 1107 84-85 -0.4487312141215192 1107 86-87 -0.5235711495679283 1107 88-89 -0.5161959386255148 1107 90-91 -0.5904362369784231 1107 92-93 -0.5755124717204332 1107 94-95 -0.5088592005902335 1107 96-97 -0.5714244849484515 1107 98-99 -0.631843437135128 1107 100 -0.5903705030246797 1108 1 -0.48701581420626283 1108 2 -0.3959990513009828 1108 3 -0.42774824691386826 1108 4 -0.3375208269237291 1108 5 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0.7797622084912348 2209 18-19 0.781258990988654 2209 20-21 0.8120063304015588 2209 22-23 0.8534432215570789 2209 24-25 0.856277638896124 2209 26-27 0.9394745574735879 2209 28-29 0.9611345926762951 2209 30-31 0.9917215911784609 2209 32-33 0.9753117762816288 2209 34-35 1.0288488887936964 2209 36-37 1.0036729152283286 2209 38-39 1.032217874133508 2209 40-41 1.082217675349881 2209 42-43 1.0699874796527453 2209 44-45 1.1408453298062895 2209 46-47 1.1043896964861872 2209 48-49 1.1633697434149681 2209 50-51 1.1326671508712778 2209 52-53 1.1131548852488038 2209 54-55 1.0654608782213089 2209 56-57 1.1053928988251513 2209 58-59 1.0532235236018863 2209 60-61 1.0746267859510823 2209 62-63 0.9937516367481223 2209 64-65 0.9990172960617585 2209 66-67 1.0438508529233488 2209 68-69 1.0590056037146809 2209 70-71 1.0773020337085555 2209 72-73 1.0373052443461148 2209 74-75 0.9996880697443515 2209 76-77 1.03638875647556 2209 78-79 1.055066076387508 2209 80-81 1.0984613653737796 2209 82-83 1.1068680063487868 2209 84-85 1.0715255542015747 2209 86-87 1.0538111323060377 2209 88-89 1.155466350015189 2209 90-91 1.1789478696383817 2209 92-93 1.1626512762987353 2209 94-95 1.13715799943839 2209 96-97 1.242644560274222 2209 98-99 1.2523082073547194 2209 100 1.3316860377417363 2210 1 0.6621706951303779 2210 2 0.6631812918545563 2210 3 0.6273948017670428 2210 4 0.5453195899571597 2210 5 0.564600258894913 2210 6 0.593910521555074 2210 7 0.5901633824016557 2210 8 0.5630285555601304 2210 9 0.7116590919467214 2210 10-11 0.7134750584518628 2210 12-13 0.6942204649517834 2210 14-15 0.8950305820944848 2210 16-17 0.9012952062112731 2210 18-19 0.8854383893054845 2210 20-21 0.911973291923772 2210 22-23 0.9767520837736541 2210 24-25 0.9824421989224064 2210 26-27 1.0120216820761954 2210 28-29 1.013500209439833 2210 30-31 1.0429626646140449 2210 32-33 1.0736197121857458 2210 34-35 1.0905862557443484 2210 36-37 1.055670415419769 2210 38-39 1.0966692130872957 2210 40-41 1.1063428658614853 2210 42-43 1.0976273466589817 2210 44-45 1.1264436412004315 2210 46-47 1.1824300392465048 2210 48-49 1.2116301286127467 2210 50-51 1.1382596697248246 2210 52-53 1.0708532828517505 2210 54-55 1.0753149284299184 2210 56-57 1.0356061853570608 2210 58-59 1.0604243679048224 2210 60-61 0.9599297889325697 2210 62-63 0.9476149772531741 2210 64-65 0.9566463437384343 2210 66-67 0.9929401642656117 2210 68-69 0.9733912362378803 2210 70-71 0.993287346098171 2210 72-73 0.9630463560692775 2210 74-75 0.971486239844964 2210 76-77 0.9684119958025903 2210 78-79 1.0256851124791133 2210 80-81 1.0736452075202578 2210 82-83 1.0864950606010488 2210 84-85 1.0495234718511632 2210 86-87 1.065478778805442 2210 88-89 1.1154334720982284 2210 90-91 1.1223027510580401 2210 92-93 1.1276068997564224 2210 94-95 1.1657823513284349 2210 96-97 1.1928807699518416 2210 98-99 1.2809528763647542 2210 100 1.4319783819545044 2211 1 0.5969988568894138 2211 2 0.5574692913433381 2211 3 0.5982055001292643 2211 4 0.48627692334050465 2211 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68-69 0.8394109091273876 2211 70-71 0.8228106937192408 2211 72-73 0.8014701007877889 2211 74-75 0.8249237513491252 2211 76-77 0.8071123091324139 2211 78-79 0.8569108321965224 2211 80-81 0.8500092619011426 2211 82-83 0.9017533059123899 2211 84-85 0.8964085282206611 2211 86-87 0.9452577531312869 2211 88-89 0.9061736590959093 2211 90-91 1.0124737210607542 2211 92-93 1.014130331422944 2211 94-95 1.0352531670625815 2211 96-97 1.0877469987485995 2211 98-99 1.1277988620131225 2211 100 1.3010474291220682 2212 1 0.48192890143448963 2212 2 0.3907667343084178 2212 3 0.4712089702151552 2212 4 0.4044861580347998 2212 5 0.27218760192612024 2212 6 0.3232056620471724 2212 7 0.4515515583458267 2212 8 0.481881673520256 2212 9 0.5910887516886021 2212 10-11 0.6123530639794268 2212 12-13 0.6534734319337687 2212 14-15 0.7999306826552939 2212 16-17 0.7881140621568221 2212 18-19 0.7720513208447173 2212 20-21 0.8147118965385047 2212 22-23 0.8559722931822265 2212 24-25 0.9014248690254547 2212 26-27 0.8791357751858584 2212 28-29 0.9340887167800602 2212 30-31 0.9110080817031445 2212 32-33 0.9159392812585878 2212 34-35 0.8650680163636153 2212 36-37 0.9295419989676716 2212 38-39 0.987956086067939 2212 40-41 1.0505119171502244 2212 42-43 1.061501485990469 2212 44-45 1.0168372051718322 2212 46-47 0.9752775662503126 2212 48-49 0.9712859481586733 2212 50-51 1.0100370066818485 2212 52-53 0.9265008902056167 2212 54-55 0.8422608930167357 2212 56-57 0.8477086627623649 2212 58-59 0.854964973164769 2212 60-61 0.8670697089440154 2212 62-63 0.8396650251510067 2212 64-65 0.9116887719368734 2212 66-67 0.8900341363134388 2212 68-69 0.8727053270471359 2212 70-71 0.8414419991442159 2212 72-73 0.8642911657678098 2212 74-75 0.9757311820909678 2212 76-77 0.8891415915711534 2212 78-79 0.9110137165516115 2212 80-81 0.9391565785321205 2212 82-83 0.973186572387096 2212 84-85 0.9749997184683288 2212 86-87 0.9939508310072327 2212 88-89 1.0538313511196336 2212 90-91 1.0848637222092457 2212 92-93 1.1123432987155404 2212 94-95 1.1739392924614798 2212 96-97 1.1216074077757412 2212 98-99 1.1273853536988625 2212 100 1.3277077175575762 2213 1 0.507397178190601 2213 2 0.4804748481567884 2213 3 0.48297208880646636 2213 4 0.42537677911904126 2213 5 0.41269628678963954 2213 6 0.4794998550935361 2213 7 0.43891917910658407 2213 8 0.47692332252488256 2213 9 0.5969758778151757 2213 10-11 0.582157472362951 2213 12-13 0.641131173148608 2213 14-15 0.7992383460154642 2213 16-17 0.7242173822350182 2213 18-19 0.7159061113760004 2213 20-21 0.7070134805144548 2213 22-23 0.8164015763120531 2213 24-25 0.8134554662512627 2213 26-27 0.7711766416964991 2213 28-29 0.8363068635759774 2213 30-31 0.8287408344463714 2213 32-33 0.9026180952563934 2213 34-35 0.9084043654010898 2213 36-37 0.9156675458835082 2213 38-39 0.9096109621867186 2213 40-41 0.9564819814663608 2213 42-43 0.9014159015319265 2213 44-45 0.9067962287415909 2213 46-47 0.8928932131140428 2213 48-49 0.9630741007886314 2213 50-51 0.9684905076517012 2213 52-53 0.8459658129967025 2213 54-55 0.8326201666687894 2213 56-57 0.7520758846343867 2213 58-59 0.7412269757582948 2213 60-61 0.8158707299615315 2213 62-63 0.8117428893259984 2213 64-65 0.8214770496687009 2213 66-67 0.8215421007278181 2213 68-69 0.8070264262067681 2213 70-71 0.805863041643633 2213 72-73 0.8358426860794523 2213 74-75 0.898542364902859 2213 76-77 0.9162981869297511 2213 78-79 0.9227343501015035 2213 80-81 0.8889054157729674 2213 82-83 0.8375470553572519 2213 84-85 0.8794055159482319 2213 86-87 0.9666867958119063 2213 88-89 1.075839159257896 2213 90-91 1.0454083146504196 2213 92-93 1.0463504665670307 2213 94-95 1.0474901451582745 2213 96-97 1.0515164518314393 2213 98-99 1.0973315644481474 2213 100 1.2579183573301762 2214 1 0.4003966753039059 2214 2 0.39317728070336955 2214 3 0.37924189836007827 2214 4 0.3287732262813421 2214 5 0.2891170449610314 2214 6 0.37624905920047524 2214 7 0.290614072483244 2214 8 0.3153554829034064 2214 9 0.34985947348601343 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0.8255845783201607 2214 78-79 0.7903509769606067 2214 80-81 0.7759761162724459 2214 82-83 0.7869784164866935 2214 84-85 0.85895509159241 2214 86-87 0.9232724171764346 2214 88-89 0.9727610693186222 2214 90-91 0.8384368800602822 2214 92-93 0.9291973577049717 2214 94-95 0.9730062494076144 2214 96-97 1.0320511484317407 2214 98-99 1.0144346427962425 2214 100 1.0878437814020288 2215 1 0.2751744778483918 2215 2 0.05400209622495211 2215 3 0.2295858084303788 2215 4 0.17843843652589442 2215 5 0.16573162102095296 2215 6 0.26240766543535443 2215 7 0.26096213544116864 2215 8 0.2955243464812298 2215 9 0.4142790173524844 2215 10-11 0.38077208110273375 2215 12-13 0.41164296984175763 2215 14-15 0.5635030077268439 2215 16-17 0.5160171233111583 2215 18-19 0.5328166559364362 2215 20-21 0.5763961623099547 2215 22-23 0.6339293487176292 2215 24-25 0.5802150081682811 2215 26-27 0.6020366595795466 2215 28-29 0.5876099809916937 2215 30-31 0.6635711971460694 2215 32-33 0.641433675366919 2215 34-35 0.6504998131850002 2215 36-37 0.6875155980862147 2215 38-39 0.7568723939179023 2215 40-41 0.7668868417774917 2215 42-43 0.658813681997195 2215 44-45 0.7265984121022058 2215 46-47 0.6763897640566867 2215 48-49 0.6748505655967847 2215 50-51 0.4438923733219511 2215 52-53 0.49006827889358107 2215 54-55 0.5601733001884597 2215 56-57 0.5818392005607578 2215 58-59 0.590041019253448 2215 60-61 0.6641761793321734 2215 62-63 0.6522911013483537 2215 64-65 0.637409991986452 2215 66-67 0.6476351307280694 2215 68-69 0.6586659968342659 2215 70-71 0.6485541879523709 2215 72-73 0.6762704569182958 2215 74-75 0.7340274912835909 2215 76-77 0.7023976443407562 2215 78-79 0.6045606221544055 2215 80-81 0.6247292783947387 2215 82-83 0.7891345465943509 2215 84-85 0.6488515404159791 2215 86-87 0.7809303597511885 2215 88-89 0.8712823189035745 2215 90-91 0.9053298143849986 2215 92-93 0.8705078446329573 2215 94-95 0.9502838592143235 2215 96-97 0.9511739240406811 2215 98-99 1.022574644420665 2215 100 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0.45217749138378593 2311 9 0.5450216079428927 2311 10-11 0.5169630246075769 2311 12-13 0.5600656645440054 2311 14-15 0.7387358636225727 2311 16-17 0.6953401558370231 2311 18-19 0.6054146052090488 2311 20-21 0.6219182321567729 2311 22-23 0.7363587630110402 2311 24-25 0.7121977151696015 2311 26-27 0.7357770890128137 2311 28-29 0.7956612367491971 2311 30-31 0.8492898142994818 2311 32-33 0.82963706867627 2311 34-35 0.7991375813068586 2311 36-37 0.7834443564691611 2311 38-39 0.894812200353158 2311 40-41 0.9295992175924681 2311 42-43 0.9365412692956951 2311 44-45 0.9238705809335741 2311 46-47 0.869541382733054 2311 48-49 0.8886646398036007 2311 50-51 0.9439857922891193 2311 52-53 0.8360199877952113 2311 54-55 0.7350615441911614 2311 56-57 0.7349612394594587 2311 58-59 0.7508527847783881 2311 60-61 0.7967168745772142 2311 62-63 0.7147122895344467 2311 64-65 0.7844113580601189 2311 66-67 0.7970097341050248 2311 68-69 0.7757142033464461 2311 70-71 0.8122809432862184 2311 72-73 0.7079022715352963 2311 74-75 0.7737393450160255 2311 76-77 0.8062441646345633 2311 78-79 0.7912048100073754 2311 80-81 0.8061486854602435 2311 82-83 0.7658496251586229 2311 84-85 0.8392186030609388 2311 86-87 0.856240719581919 2311 88-89 0.8516186619285087 2311 90-91 0.933424322683333 2311 92-93 0.9103753743727552 2311 94-95 0.9087873303884066 2311 96-97 0.9486533223589291 2311 98-99 0.9906068903337015 2311 100 1.0907068123471149 2312 1 0.44347947466058457 2312 2 0.4315429976286822 2312 3 0.4528451905603035 2312 4 0.39372090793039405 2312 5 0.38122812180379384 2312 6 0.4359492494590995 2312 7 0.44055728723884613 2312 8 0.41282468433812625 2312 9 0.5348640955568982 2312 10-11 0.5530949320498593 2312 12-13 0.5683407681500796 2312 14-15 0.7175951062633175 2312 16-17 0.6710382309452996 2312 18-19 0.6847442205200451 2312 20-21 0.7169334002634642 2312 22-23 0.7329518092743186 2312 24-25 0.7358841521223596 2312 26-27 0.7933697251919227 2312 28-29 0.7920519117577598 2312 30-31 0.8138638136273215 2312 32-33 0.78942959501736 2312 34-35 0.808257335901807 2312 36-37 0.8850870857935789 2312 38-39 0.9181901421646046 2312 40-41 0.9137072316499584 2312 42-43 0.9272442381493349 2312 44-45 0.8956838318271849 2312 46-47 0.9443127900173067 2312 48-49 0.9515831955283076 2312 50-51 0.9019576172249018 2312 52-53 0.7867166162021917 2312 54-55 0.758703801111956 2312 56-57 0.7440473999809782 2312 58-59 0.756953245942249 2312 60-61 0.7525181606946774 2312 62-63 0.7488548296748476 2312 64-65 0.7815282214781618 2312 66-67 0.769846734826146 2312 68-69 0.8485385593417973 2312 70-71 0.8207732746564815 2312 72-73 0.8377449372048531 2312 74-75 0.8094013110163161 2312 76-77 0.8091949484128165 2312 78-79 0.8611883871655159 2312 80-81 0.8250847535041714 2312 82-83 0.8510979597828587 2312 84-85 0.8788679421410173 2312 86-87 0.8879008454894439 2312 88-89 0.9028898811415935 2312 90-91 1.0122861666042944 2312 92-93 1.0159023019353093 2312 94-95 1.084860226328292 2312 96-97 1.1196866204150062 2312 98-99 1.1716571342697968 2312 100 1.252240666270346 2313 1 0.4835266744761597 2313 2 0.4239838158966478 2313 3 0.4464788371807842 2313 4 0.35728274447504305 2313 5 0.3416371913562628 2313 6 0.38140432368000177 2313 7 0.40935617640339217 2313 8 0.40127861070800463 2313 9 0.5318226301818996 2313 10-11 0.5240977897117887 2313 12-13 0.524995815409369 2313 14-15 0.729273987786847 2313 16-17 0.676247927227827 2313 18-19 0.6505055384501404 2313 20-21 0.658189617435859 2313 22-23 0.6509358105872778 2313 24-25 0.6777870532853854 2313 26-27 0.6840785023156712 2313 28-29 0.733174931622699 2313 30-31 0.8328195782439423 2313 32-33 0.8086948110529519 2313 34-35 0.8084661503385107 2313 36-37 0.8767059394945349 2313 38-39 0.90003620763828 2313 40-41 0.9438952995218486 2313 42-43 0.7674233112645794 2313 44-45 0.9427193146694606 2313 46-47 0.9554926285789804 2313 48-49 0.9181458302301664 2313 50-51 0.90374194782639 2313 52-53 0.7939324320911965 2313 54-55 0.7908175834382831 2313 56-57 0.7606131181341311 2313 58-59 0.723288436285003 2313 60-61 0.7580623813511238 2313 62-63 0.8094345227655992 2313 64-65 0.801870981605326 2313 66-67 0.7830905032808317 2313 68-69 0.8322817438878118 2313 70-71 0.7962194617406269 2313 72-73 0.8097983897356968 2313 74-75 0.7896296266008704 2313 76-77 0.8079914377247448 2313 78-79 0.8380227734036545 2313 80-81 0.8636473830374456 2313 82-83 0.8543030056119356 2313 84-85 0.8794188656753867 2313 86-87 0.8561989216282484 2313 88-89 0.8679436071827169 2313 90-91 0.9210691899782013 2313 92-93 0.9444500799245468 2313 94-95 0.9548614951100873 2313 96-97 1.0831549179335767 2313 98-99 1.1532111392021882 2313 100 1.1962015941633233 2314 1 0.3339240972633277 2314 2 0.25012740285308865 2314 3 0.31063442677383435 2314 4 0.2681267930787996 2314 5 0.19784254375995403 2314 6 0.276755908419581 2314 7 0.3273080927328422 2314 8 0.3115906177405421 2314 9 0.44085691628175283 2314 10-11 0.4909169116784611 2314 12-13 0.49553760386542933 2314 14-15 0.5972968675933856 2314 16-17 0.6059819456340279 2314 18-19 0.5947024552150211 2314 20-21 0.6052965856161663 2314 22-23 0.6614588332912277 2314 24-25 0.7174524498828561 2314 26-27 0.7638646076578652 2314 28-29 0.6951815154059773 2314 30-31 0.7287061566652397 2314 32-33 0.7559893824627508 2314 34-35 0.7502049642955342 2314 36-37 0.7634941006084475 2314 38-39 0.8267270497147337 2314 40-41 0.7842020904805125 2314 42-43 0.734703624150292 2314 44-45 0.7896226050392343 2314 46-47 0.682110377926243 2314 48-49 0.6561805488481411 2314 50-51 0.7357944893534665 2314 52-53 0.7277212845588465 2314 54-55 0.7206579838611944 2314 56-57 0.7522440078512034 2314 58-59 0.6615708547889838 2314 60-61 0.6103906328637407 2314 62-63 0.7479227025236099 2314 64-65 0.7208425000673131 2314 66-67 0.7568626216931094 2314 68-69 0.7876739968338669 2314 70-71 0.7565183471509442 2314 72-73 0.8175078984310531 2314 74-75 0.8176427079454385 2314 76-77 0.7487562036682718 2314 78-79 0.8226519780594437 2314 80-81 0.8166775149409631 2314 82-83 0.8101977493333727 2314 84-85 0.862892429347653 2314 86-87 0.8622318563570985 2314 88-89 0.7095102240248821 2314 90-91 0.6793622113094209 2314 92-93 0.90447439785002 2314 94-95 0.9864290277851602 2314 96-97 0.9688844590061123 2314 98-99 0.9957027846022051 2314 100 1.00774154004435 2315 1 0.26729041688568245 2315 2 0.3179851787546788 2315 3 0.31378386961824845 2315 4 0.19996580163432753 2315 5 0.2171010218395395 2315 6 0.175969454788941 2315 7 0.20654513567263422 2315 8 0.2433571788204958 2315 9 0.1498191601515444 2315 10-11 0.2909569294342944 2315 12-13 0.28882995840498893 2315 14-15 0.4316198320835909 2315 16-17 0.2954756341384339 2315 18-19 0.44738985633900796 2315 20-21 0.302284500670261 2315 22-23 0.30594755260918305 2315 24-25 0.47035110091187704 2315 26-27 0.49320141245398474 2315 28-29 0.45179518563112 2315 30-31 0.5035628719412202 2315 32-33 0.5745976134340722 2315 34-35 0.48293113578039737 2315 36-37 0.6275118246632658 2315 38-39 0.6594285537093754 2315 40-41 0.7062712943893175 2315 42-43 0.6356827824577991 2315 44-45 0.6535021384659387 2315 46-47 0.738649854492806 2315 48-49 0.6531754911204359 2315 50-51 0.5884363580122951 2315 52-53 0.2678889402193718 2315 54-55 0.48858132447326597 2315 56-57 0.4055329668933112 2315 58-59 0.5053134866190021 2315 60-61 0.6214039917159866 2315 62-63 0.6203409178549038 2315 64-65 0.6679851541261073 2315 66-67 0.5013521737406634 2315 68-69 0.5612789453210425 2315 70-71 0.49412525306252775 2315 72-73 0.6012512321331656 2315 74-75 0.39439589266882535 2315 76-77 0.5847844241947868 2315 78-79 0.6651122742766553 2315 80-81 0.6402987144012897 2315 82-83 0.6633092944835433 2315 84-85 0.6885456674846999 2315 86-87 0.6169529573683903 2315 88-89 0.5606722132153728 2315 90-91 0.7293785175146326 2315 92-93 0.8221955636102827 2315 94-95 0.7676522922789424 2315 96-97 0.8248169054314332 2315 98-99 0.9599130433890757 2315 100 0.9654018961501194 2316 1 0.11095223157177969 2316 2 0.11825108833781783 2316 3 0.1402597489799966 2316 4 0.009431671562467159 2316 5 -0.020813391060912068 2316 6 -0.1417287658137667 2316 7 -0.11575278876109962 2316 8 -0.07544830752495812 2316 9 0.05868574905111501 2316 10-11 0.05138696340011961 2316 12-13 0.03413127251232595 2316 14-15 -0.07357599212040355 2316 16-17 0.05058532615931455 2316 18-19 0.13314442745876676 2316 20-21 0.19777566137008762 2316 22-23 0.08111893613695287 2316 24-25 0.06684694421797133 2316 26-27 -0.03855064603092728 2316 28-29 0.04265937968639122 2316 30-31 -0.5382973690492676 2316 32-33 -0.39428206196141957 2316 34-35 -0.5019766473426799 2316 36-37 -0.23286140385834386 2316 38-39 -0.1741173150028601 2316 40-41 0.06125468108533738 2316 42-43 0.13511750006616552 2316 44-45 0.009561693853235909 2316 46-47 -0.13264348232703327 2316 48-49 -0.049531895383530866 2316 50-51 0.08936495172089565 2316 52-53 -0.2038381836289247 2316 54-55 -0.17338308689003412 2316 56-57 -0.16497897986673848 2316 58-59 -0.4926196173278754 2316 60-61 -0.07855989390765927 2316 62-63 -0.06467865402997575 2316 64-65 -0.3655466125352902 2316 66-67 -0.6388451737957013 2316 68-69 -0.6900913370550441 2316 70-71 -0.4676111506778966 2316 72-73 -0.6895900342154562 2316 74-75 -0.49752826092313995 2316 76-77 -0.16696465325647836 2316 78-79 -0.15071618537178466 2316 80-81 -0.20661743565074886 2316 82-83 -0.004295039103446641 2316 84-85 0.14024691195398375 2316 86-87 0.24149855699312184 2316 88-89 0.21461712592428484 2316 90-91 -0.2825284493648077 2316 92-93 -0.34277972628055764 2316 94-95 -0.0245622184809271 2316 96-97 0.12165648298822163 2316 98-99 0.2546499645789062 2316 100 0.06331116564488681 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 260.0 1 698.0 2 381.0 3 372.0 4 368.0 5 363.0 6 354.0 7 793.0 8 2190.0 9 4223.0 10 6718.0 11 11254.0 12 16293.0 13 20533.0 14 23311.0 15 26759.0 16 31084.0 17 37497.0 18 45083.0 19 55218.0 20 68409.0 21 84720.0 22 96843.0 23 98413.0 24 98269.0 25 100894.0 26 110355.0 27 126085.0 28 148432.0 29 177803.0 30 218091.0 31 270683.0 32 343711.0 33 451436.0 34 629887.0 35 884542.0 36 1308864.0 37 1812084.0 38 1561347.0 39 365775.0 40 212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93349511531214 19.258799100576198 13.648235579500644 23.15947020461101 2 15.808497290057014 19.680960263089105 40.467098857857806 24.04344358899608 3 19.203515771583948 25.507008131677566 29.054081625032346 26.235394471706137 4 13.087520583852994 16.32667816714251 37.67406303556712 32.911738213437374 5 14.018393881564911 37.326662360928054 33.946560133731175 14.708383623775859 6 31.39101090020917 36.40874485195723 17.621755812795296 14.578488435038302 7 28.207102809566464 32.10506691160756 21.63643123368838 18.051399045137593 8 26.54299322650126 34.659380745803695 19.65065946562023 19.146966562074816 9 26.686451672966076 16.016177300791814 20.488588258791346 36.80878276745076 10-11 25.67261039867303 25.92261326747855 27.038681458011403 21.366094875837014 12-13 26.093922110652045 23.556485718012826 27.60757837623081 22.742013795104324 14-15 23.549698241365988 24.8309234752748 25.395837981415465 26.223540301943743 16-17 22.62456376166611 27.84760724630487 26.248798793426463 23.279030198602555 18-19 22.382523182010168 26.929005934553462 28.577096662739958 22.111374220696405 20-21 23.47679776886393 25.987978919666183 28.373827025998754 22.161396285471127 22-23 23.542259181243267 25.77200960283007 28.120401434112413 22.56532978181425 24-25 23.075758552000725 26.009095206201504 28.382479464315203 22.532666777482568 26-27 22.889992537271414 26.218789617321846 28.13462753707867 22.756590308328068 28-29 22.58973974050112 26.254259661182516 28.44160078753419 22.71439981078218 30-31 23.035005051392545 26.08325600063317 28.279878220892073 22.601860727082208 32-33 23.032958219460834 26.375188070294037 27.916040938560222 22.675812771684907 34-35 23.122955147232645 26.299861738057796 28.189526423751282 22.387656690958277 36-37 23.085450343920055 26.12042912735192 27.996656881621888 22.797463647106138 38-39 23.0798188144445 26.149411769482388 28.204381853836253 22.56638756223686 40-41 22.960027303402455 25.858886149814207 28.410188578199353 22.770897968583977 42-43 22.854761348158686 25.989636011967377 28.097716178361164 23.057886461512776 44-45 22.900126186931104 26.350599995657873 27.932330194063233 22.816943623347793 46-47 22.951033044673714 26.296789068263582 27.586699401612325 23.16547848545038 48-49 23.257262680081496 26.22549622065055 27.54473579204455 22.9725053072234 50-51 23.119611667010805 26.383435191231648 27.272718905874772 23.224234235882772 52-53 22.90877715173917 26.666793723693306 27.2198707949155 23.20455832965202 54-55 22.87622726436562 26.56762149645183 27.22724953217812 23.328901707004427 56-57 23.120699344161828 26.46480270174576 27.039914417287186 23.374583536805222 58-59 22.90021322111682 26.45392196112538 27.36516139650222 23.280703421255577 60-61 22.941549528519577 26.66969465088903 27.148599769328236 23.24015605126316 62-63 22.964898588173437 26.56434818500788 27.069821032348095 23.400932194470588 64-65 22.708517053439618 26.421766919082234 27.24099640419746 23.628719623280688 66-67 22.942631862111963 26.31653143444111 27.105436310564464 23.635400392882467 68-69 22.7139971489966 26.40157460278961 27.178210451260888 23.706217796952902 70-71 22.775924521784606 26.156185852084633 27.255062736542797 23.812826889587964 72-73 23.04478886123583 26.37163640663454 27.030998377488235 23.552576354641396 74-75 23.023758831307255 26.5570396448984 26.9688082104035 23.450393313390848 76-77 22.91843018586166 26.476250986109047 26.85777205511513 23.747546772914163 78-79 22.86282058591336 26.393427187800274 26.933653729069928 23.810098497216433 80-81 22.758133440911664 26.685783802101643 26.95402396245558 23.60205879453111 82-83 23.10155258342168 26.33050079155324 27.249809136108503 23.318137488916577 84-85 23.142787068704614 26.421820410465923 26.81258977283986 23.622802747989603 86-87 22.73570894172731 26.663054818787796 27.07113912834781 23.530097111137085 88-89 22.986306805057986 26.13940937477767 27.049249739051678 23.82503408111267 90-91 23.08683825027217 26.186068613732644 27.030471516495545 23.696621619499638 92-93 23.11780762486864 26.27822766696772 26.94368185385404 23.6602828543096 94-95 22.718812802083573 26.11381844032711 27.209561144076076 23.957807613513243 96-97 22.886433089236462 26.02964846964068 27.08344625757991 24.000472183542943 98-99 22.744532159790385 26.2160269107331 26.959298577607722 24.080142351868798 100 23.13837795935466 25.955471970521394 26.510297975036245 24.395852095087704 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2038.0 1 1471.0 2 1505.5 3 2017.0 4 1565.5 5 1420.0 6 2182.0 7 3906.0 8 6546.0 9 8183.0 10 7466.5 11 6413.0 12 6642.0 13 7391.0 14 8125.0 15 9023.0 16 9889.5 17 10754.0 18 11536.0 19 12205.0 20 13079.0 21 14096.0 22 15726.5 23 18076.0 24 20792.5 25 24155.5 26 29177.5 27 35382.0 28 42951.0 29 54761.0 30 71563.5 31 89023.0 32 111208.5 33 136889.0 34 160940.0 35 184183.5 36 211507.5 37 245430.5 38 261871.5 39 260612.5 40 257806.5 41 254944.0 42 259890.5 43 275954.0 44 295079.5 45 321831.0 46 347149.5 47 362458.5 48 376409.5 49 382043.0 50 378474.0 51 376175.0 52 371441.5 53 363579.0 54 353195.5 55 329810.0 56 299989.0 57 263766.0 58 230382.5 59 205774.5 60 174934.5 61 142550.0 62 116002.5 63 93464.5 64 71350.5 65 51487.0 66 37983.0 67 29122.0 68 22522.0 69 17005.0 70 13482.5 71 10596.0 72 8198.5 73 6506.5 74 5081.5 75 3939.0 76 2984.5 77 2038.5 78 1312.0 79 899.5 80 627.0 81 436.5 82 305.5 83 209.5 84 152.5 85 123.5 86 91.0 87 66.0 88 61.5 89 52.0 90 39.0 91 33.5 92 24.5 93 20.0 94 19.0 95 16.0 96 14.5 97 10.5 98 4.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024327406197450017 2 0.10789334510168001 3 0.016817077059981016 4 0.016979404058629483 5 0.01919787304015851 6 0.04422869623175188 7 0.04429362703121126 8 0.03712959549085899 9 0.03216238933221595 10-11 0.03585262310149106 12-13 0.03540892930518526 14-15 0.03166458653636065 16-17 0.03831458258099279 18-19 0.037156649990633735 20-21 0.047783657502153264 22-23 0.047307498306117766 24-25 0.0657099690528988 26-27 0.058210461715339695 28-29 0.05075965247737514 30-31 0.04913097159093553 32-33 0.0436551408365273 34-35 0.04494834592576007 36-37 0.040278739264639214 38-39 0.04566258471981332 40-41 0.05549418993795538 42-43 0.055905418334531484 44-45 0.05950366680457247 46-47 0.06086180269326463 48-49 0.060082633099751995 50-51 0.05441200994696561 52-53 0.06394060476763053 54-55 0.05107889557471711 56-57 0.05515871414074854 58-59 0.05291319065944477 60-61 0.049125560690980584 62-63 0.05621925053191852 64-65 0.05386550905151577 66-67 0.0515766983705724 68-69 0.056944311125881664 70-71 0.057999436617096695 72-73 0.05998523690056292 74-75 0.05102478657516763 76-77 0.052085322966337604 78-79 0.06252294897943392 80-81 0.0626095233787131 82-83 0.05376270195237175 84-85 0.05943332510515814 86-87 0.056928078426016826 88-89 0.06514723545758412 90-91 0.07042827381361419 92-93 0.0748165136770777 94-95 0.07829572234810982 96-97 0.07477322647743812 98-99 0.08344689910522111 100 0.12929886532345766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9240607.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.86728344547477 #Duplication Level Percentage of deduplicated Percentage of total 1 70.7608334280835 36.701722042524324 2 15.301963746123 15.873425817850812 3 5.773002713325354 8.982899041906233 4 2.844148238923344 5.900729714767397 5 1.56002646713784 4.0457167476740485 6 0.9417721631326194 2.9308298235754475 7 0.6097911610345155 2.2139747694345653 8 0.43919067377238125 1.822370173052891 9 0.2953388099953557 1.3786579593430497 >10 1.3693237403854175 11.989493917156272 >50 0.06835234808231214 2.407183323135945 >100 0.033714408911959114 3.1626980687254407 >500 0.0017670819024299647 0.6512207255248713 >1k 6.510161197589218E-4 0.5708779048518422 >5k 3.1000767556148944E-5 0.11220104821881421 >10k+ 9.300230266844684E-5 1.2559989222579873 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34879 0.37745355905732164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27666 0.2993959163072296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15568 0.1684737809972873 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.246539972969308E-5 2 0.0 0.0 0.0 0.0 3.246539972969308E-5 3 0.0 0.0 0.0 0.0 3.246539972969308E-5 4 0.0 0.0 0.0 0.0 4.3287199639590775E-5 5 1.0821799909897694E-5 0.0 0.0 0.0 4.3287199639590775E-5 6 1.0821799909897694E-5 0.0 0.0 0.0 5.410899954948847E-5 7 1.0821799909897694E-5 0.0 0.0 0.0 6.493079945938616E-5 8 1.0821799909897694E-5 0.0 0.0 1.0821799909897694E-5 8.657439927918155E-5 9 1.0821799909897694E-5 0.0 0.0 4.3287199639590775E-5 8.657439927918155E-5 10-11 1.0821799909897694E-5 0.0 0.0 4.3287199639590775E-5 8.657439927918155E-5 12-13 1.623269986484654E-5 0.0 0.0 5.410899954948847E-5 8.657439927918155E-5 14-15 2.1643599819795388E-5 0.0 0.0 6.493079945938616E-5 1.0821799909897694E-4 16-17 2.1643599819795388E-5 0.0 0.0 7.575259936928386E-5 1.1903979900887463E-4 18-19 2.1643599819795388E-5 0.0 0.0 8.116349932423271E-5 1.4068339882867002E-4 20-21 2.1643599819795388E-5 0.0 0.0 1.7855969851331195E-4 1.4068339882867002E-4 22-23 2.1643599819795388E-5 0.0 0.0 3.6253029698157276E-4 1.6773789860341425E-4 24-25 2.1643599819795388E-5 0.0 0.0 8.008131933324293E-4 2.4890139792764694E-4 26-27 2.1643599819795388E-5 0.0 0.0 0.0017693642852682728 2.7054499774744234E-4 28-29 2.1643599819795388E-5 0.0 0.0 0.004447759762967952 2.7054499774744234E-4 30-31 2.1643599819795388E-5 0.0 0.0 0.013029447091516823 2.759558977023912E-4 32-33 2.1643599819795388E-5 0.0 0.0 0.034332160214150434 3.246539972969308E-4 34-35 2.1643599819795388E-5 5.410899954948847E-6 0.0 0.06714385754096025 3.246539972969308E-4 36-37 3.246539972969308E-5 1.0821799909897694E-5 0.0 0.121009366592476 3.3006489725187965E-4 38-39 3.246539972969308E-5 1.0821799909897694E-5 0.0 0.20118808212490802 3.6253029698157276E-4 40-41 3.246539972969308E-5 1.0821799909897694E-5 0.0 0.2870969407096309 3.841738968013681E-4 42-43 3.246539972969308E-5 1.623269986484654E-5 0.0 0.37486714887885614 3.949956967112658E-4 44-45 3.246539972969308E-5 2.1643599819795388E-5 0.0 0.4819218044875191 4.3287199639590777E-4 46-47 3.246539972969308E-5 3.246539972969308E-5 0.0 0.6116427200074628 4.5451559621570317E-4 48-49 3.246539972969308E-5 3.246539972969308E-5 0.0 0.753148575629285 4.978027958552939E-4 50-51 3.246539972969308E-5 3.246539972969308E-5 0.0 0.8966564642344383 4.978027958552939E-4 52-53 3.246539972969308E-5 3.246539972969308E-5 0.0 1.0392390889472953 5.519117954047824E-4 54-55 3.246539972969308E-5 3.246539972969308E-5 0.0 1.1952082801486958 6.114316949092197E-4 56-57 3.246539972969308E-5 3.246539972969308E-5 0.0 1.3794764781144788 6.168425948641686E-4 58-59 3.246539972969308E-5 3.787629968464193E-5 0.0 1.589013578869873 6.222534948191174E-4 60-61 3.246539972969308E-5 5.410899954948847E-5 0.0 1.8043403425770623 6.384861946839639E-4 62-63 3.246539972969308E-5 5.9519899504437315E-5 0.0 2.0217935899665465 6.925951942334524E-4 64-65 3.246539972969308E-5 6.493079945938616E-5 0.0 2.2458264917012487 6.925951942334524E-4 66-67 3.787629968464193E-5 6.493079945938616E-5 0.0 2.4968814278109654 6.980060941884013E-4 68-69 4.3287199639590775E-5 6.493079945938616E-5 0.0 2.7749475764957863 7.034169941433501E-4 70-71 4.3287199639590775E-5 6.493079945938616E-5 0.0 3.074787186599322 7.358823938730432E-4 72-73 4.3287199639590775E-5 6.493079945938616E-5 0.0 3.3741506375068218 7.791695935126339E-4 74-75 4.3287199639590775E-5 6.493079945938616E-5 0.0 3.671284797632883 7.791695935126339E-4 76-77 4.3287199639590775E-5 6.493079945938616E-5 0.0 3.998563081407964 7.791695935126339E-4 78-79 5.410899954948847E-5 7.034169941433501E-5 0.0 4.35324757345486 7.791695935126339E-4 80-81 5.410899954948847E-5 7.575259936928386E-5 0.0 4.731009553809614 8.008131933324293E-4 82-83 6.493079945938616E-5 7.575259936928386E-5 0.0 5.11813239108643 8.549221928819178E-4 84-85 6.493079945938616E-5 7.575259936928386E-5 0.0 5.499011049815234 8.549221928819178E-4 86-87 6.493079945938616E-5 7.575259936928386E-5 0.0 5.89241053103979 8.765657927017132E-4 88 6.493079945938616E-5 8.657439927918155E-5 0.0 6.2016488743650715 8.873875926116109E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17285 0.0 51.944447 1 GTATCAA 31810 0.0 41.72496 1 CAACGCA 38505 0.0 33.45864 5 ATCAACG 38690 0.0 33.274364 3 TATCAAC 40070 0.0 32.74678 2 TCAACGC 40830 0.0 31.656443 4 AACGCAG 41160 0.0 31.643211 6 ACGCAGA 46110 0.0 27.931574 7 CGCAGAG 47160 0.0 27.27891 8 GTGGTAT 7605 0.0 26.655119 1 GTACTAG 3845 0.0 26.054688 1 GTACATG 43660 0.0 25.627916 1 TACATGG 44680 0.0 24.810196 2 GCAGAGT 51080 0.0 24.671951 9 ACATGGG 45390 0.0 23.756392 3 GAGTACT 26850 0.0 23.057453 12-13 TGGTATC 8860 0.0 21.869925 2 GTACTTT 28785 0.0 21.329866 14-15 CATGGGG 30925 0.0 21.13335 4 CAGAGTA 50595 0.0 20.929482 10-11 >>END_MODULE