FastQCFastQC Report
Mon 6 Jun 2016
ERR1211277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9240607
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307150.33239158423250764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259120.2804144792652691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154770.1674889972054866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176450.053.514161
GTATCAA312600.040.910981
CAACGCA394900.031.5637175
ATCAACG395500.031.49913
TATCAAC406450.031.0990452
TCAACGC417850.029.915664
AACGCAG424300.029.7634586
GTACTAG37650.028.6698381
GTGGTAT70450.027.9789031
ACGCAGA479050.025.9701237
CGCAGAG493750.025.1874378
GTACATG439350.024.0024091
TACATGG444850.023.4256462
GCAGAGT533650.023.0573319
ACATGGG450550.022.443183
TGGTATC87200.021.8457072
GAGTACT280700.021.33908712-13
TAGTACT52850.021.3084094
CAGAGTA523500.020.40226410-11
CATGGGG306500.020.1623024
Produced by FastQC (version 0.11.5)