##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211277_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9240607 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01771052486054 33.0 31.0 34.0 30.0 34.0 2 32.163396084261564 33.0 31.0 34.0 30.0 34.0 3 32.21936199645759 34.0 31.0 34.0 30.0 34.0 4 35.8846032517128 37.0 35.0 37.0 35.0 37.0 5 35.7805908204948 37.0 35.0 37.0 35.0 37.0 6 35.79719254373658 37.0 35.0 37.0 35.0 37.0 7 35.7085275891508 37.0 35.0 37.0 33.0 37.0 8 35.69157145196198 37.0 35.0 37.0 33.0 37.0 9 37.28356297373105 39.0 37.0 39.0 34.0 39.0 10-11 37.23268438967267 39.0 37.0 39.0 33.0 39.0 12-13 37.15390255207261 39.0 37.0 39.0 33.0 39.0 14-15 38.351005891712525 40.0 38.0 41.0 33.0 41.0 16-17 38.33388418098508 40.0 38.0 41.0 33.0 41.0 18-19 38.34198933035459 40.0 38.0 41.0 33.5 41.0 20-21 38.310557628952296 40.0 38.0 41.0 34.0 41.0 22-23 38.19798980737954 40.0 38.0 41.0 33.0 41.0 24-25 38.09671539975675 40.0 37.0 41.0 33.0 41.0 26-27 37.985529467923485 40.0 37.0 41.0 33.0 41.0 28-29 37.88098373840593 40.0 37.0 41.0 33.0 41.0 30-31 37.884067572617255 40.0 37.0 41.0 33.0 41.0 32-33 37.81456521200393 40.0 37.0 41.0 33.0 41.0 34-35 37.675127023581894 40.0 37.0 41.0 33.0 41.0 36-37 37.51977689344434 40.0 36.0 41.0 32.0 41.0 38-39 37.39472596334851 40.0 36.0 41.0 32.0 41.0 40-41 37.27379359386239 39.0 36.0 41.0 31.0 41.0 42-43 37.153799636755465 39.0 36.0 41.0 31.0 41.0 44-45 36.97130280510793 39.0 35.5 41.0 31.0 41.0 46-47 36.69899818269514 39.0 35.0 41.0 30.0 41.0 48-49 35.13185475802618 38.0 34.5 40.0 24.0 41.0 50-51 35.827699143573575 38.0 34.5 40.0 28.0 41.0 52-53 35.900677358099955 38.0 35.0 40.0 28.5 41.0 54-55 35.71209586123509 38.0 34.5 40.0 28.0 41.0 56-57 35.47533354681137 38.0 34.0 40.0 28.0 41.0 58-59 35.215801353742236 37.5 34.0 40.0 27.0 41.0 60-61 34.95099764550099 37.0 34.0 40.0 27.0 41.0 62-63 34.619324358237506 36.5 33.0 39.5 26.0 41.0 64-65 34.275156870106045 36.0 33.0 39.0 26.0 40.5 66-67 33.932492584091065 35.5 33.0 39.0 26.0 40.0 68-69 33.57195836810287 35.0 32.0 38.0 25.5 40.0 70-71 33.22274916572039 35.0 32.0 37.5 25.0 40.0 72-73 32.85764468719425 35.0 32.0 37.0 25.0 39.0 74-75 32.50416644707431 35.0 31.0 36.5 24.0 39.0 76-77 31.61954836949564 34.0 30.5 35.5 23.5 38.0 78-79 32.07983707130928 34.0 31.0 35.5 24.0 37.5 80-81 31.97250840772689 34.0 31.0 35.0 24.0 37.0 82-83 31.72115186805369 34.0 31.0 35.0 24.0 37.0 84-85 31.450582467147452 34.0 31.0 35.0 23.5 36.0 86-87 31.197824287949913 34.0 31.0 35.0 23.0 36.0 88-89 30.973737114888664 34.0 30.0 35.0 21.5 35.5 90-91 30.755875074007584 34.0 30.0 35.0 20.0 35.0 92-93 30.56057280652667 34.0 30.0 35.0 20.0 35.0 94-95 30.36403079364808 34.0 29.0 35.0 20.0 35.0 96-97 30.169142460013717 34.0 29.0 35.0 19.0 35.0 98-99 29.94513439430981 33.0 29.0 35.0 19.0 35.0 100 27.454008270235928 31.0 24.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3414148359041711 1101 2 -0.288883039608848 1101 3 -0.3423858819863703 1101 4 -0.17946676498437597 1101 5 -0.21757630229333103 1101 6 -0.23453565293868195 1101 7 -0.23964013029563347 1101 8 -0.268973708273478 1101 9 -0.4508517579933482 1101 10-11 -0.38509592173378593 1101 12-13 -0.42529166453616796 1101 14-15 -0.5184275334928969 1101 16-17 -0.5258363131178854 1101 18-19 -0.5939196443996551 1101 20-21 -0.6091695471194924 1101 22-23 -0.7100912281772835 1101 24-25 -0.640407915204257 1101 26-27 -0.6667599150526016 1101 28-29 -0.7482978414544661 1101 30-31 -0.6466193315682958 1101 32-33 -0.5776329630937269 1101 34-35 -0.6636504265711451 1101 36-37 -0.6927389919019546 1101 38-39 -0.6092651451917916 1101 40-41 -0.6602297194289903 1101 42-43 -0.7044865652028989 1101 44-45 -0.7010016691634817 1101 46-47 -0.7418878408183716 1101 48-49 0.446284441127041 1101 50-51 -0.40971631679854426 1101 52-53 -0.7272419278389961 1101 54-55 -0.7075462992636332 1101 56-57 -0.7430148778623931 1101 58-59 -0.90905361077197 1101 60-61 -0.8752045378692017 1101 62-63 -0.8554531694319678 1101 64-65 -0.9378109288859733 1101 66-67 -0.8739008604875238 1101 68-69 -0.8761869338699739 1101 70-71 -0.8960286630429906 1101 72-73 -1.050465814689776 1101 74-75 -1.0470378951519592 1101 76-77 -0.9014812123055655 1101 78-79 -0.8855229728421321 1101 80-81 -0.7990085625075523 1101 82-83 -0.8105123314323102 1101 84-85 -0.8306803477725921 1101 86-87 -0.8698621240758904 1101 88-89 -0.8120483986102585 1101 90-91 -0.9252454819223956 1101 92-93 -0.9798054074802671 1101 94-95 -0.9432260104490737 1101 96-97 -1.0402091858001512 1101 98-99 -1.1097790729818051 1101 100 -0.9337492630822268 1102 1 -0.23575863012986176 1102 2 -0.24650389263967298 1102 3 -0.26652244664429503 1102 4 -0.14871406138429677 1102 5 -0.15611991226638366 1102 6 -0.24953417755423146 1102 7 -0.21142944864204338 1102 8 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1105 68-69 -0.911981385120697 1105 70-71 -0.8238321081718993 1105 72-73 -0.855640553990014 1105 74-75 -0.8669779519412728 1105 76-77 -0.8476635353374462 1105 78-79 -0.7546536333198723 1105 80-81 -0.7665840595099134 1105 82-83 -0.7931850719864393 1105 84-85 -0.8182216168469552 1105 86-87 -0.7260388007698388 1105 88-89 -0.7648830232198591 1105 90-91 -0.799607992155785 1105 92-93 -0.7806244859672411 1105 94-95 -0.7878898855816026 1105 96-97 -0.8379650780269969 1105 98-99 -0.8590573434960369 1105 100 -0.9455409065020852 1106 1 -0.314647786304306 1106 2 -0.3234427609332755 1106 3 -0.32386068513299904 1106 4 -0.23366636655239859 1106 5 -0.2703545525947959 1106 6 -0.3742610977724894 1106 7 -0.367984747494738 1106 8 -0.3812823834304524 1106 9 -0.47523643764118617 1106 10-11 -0.4709665182855005 1106 12-13 -0.5000436368500587 1106 14-15 -0.6467808923803133 1106 16-17 -0.6415301952591363 1106 18-19 -0.5838012110614059 1106 20-21 -0.6452722473088741 1106 22-23 -0.655388436129293 1106 24-25 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2311 12-13 0.49683112198746215 2311 14-15 0.5165732717782632 2311 16-17 0.5756652118006755 2311 18-19 0.6699846363810735 2311 20-21 0.7127542151073811 2311 22-23 0.7415286410222137 2311 24-25 0.6882510228979157 2311 26-27 0.718668091666828 2311 28-29 0.7864769782259415 2311 30-31 0.6456298315613012 2311 32-33 0.6861322300286403 2311 34-35 0.8400711493954844 2311 36-37 0.882636070786873 2311 38-39 0.9543730393396643 2311 40-41 0.8728657531122792 2311 42-43 0.889214430479214 2311 44-45 0.9212989905472639 2311 46-47 0.9431508325840312 2311 48-49 -5.725394274027138 2311 50-51 -0.8274638816177813 2311 52-53 0.5271197367686256 2311 54-55 0.926128204016571 2311 56-57 1.0232775415799082 2311 58-59 1.0508875636599626 2311 60-61 0.916309840808033 2311 62-63 0.8590978256385569 2311 64-65 0.89598986945375 2311 66-67 1.0898051604943788 2311 68-69 1.0838041877606344 2311 70-71 1.1297173340458002 2311 72-73 0.5798671190659732 2311 74-75 0.9366362003960162 2311 76-77 0.9974107243817762 2311 78-79 0.9098509481964427 2311 80-81 0.9472510039099831 2311 82-83 0.9842735887581817 2311 84-85 1.046577622373249 2311 86-87 1.0822915685510885 2311 88-89 1.0474049751701102 2311 90-91 1.1580471801205867 2311 92-93 1.141064214021899 2311 94-95 1.1773622248450444 2311 96-97 1.2403265947420508 2311 98-99 1.2218783279698435 2311 100 1.4475327693814144 2312 1 0.10140900617088278 2312 2 0.2414278997516064 2312 3 0.2505214261968405 2312 4 0.014140341466763573 2312 5 0.20758072534952987 2312 6 0.11353889798260042 2312 7 0.31054634337640863 2312 8 0.35808590762181325 2312 9 0.27217594697243186 2312 10-11 0.26658606222187586 2312 12-13 0.35185757436428133 2312 14-15 0.384437779298878 2312 16-17 0.5254711173466546 2312 18-19 0.37048264939247844 2312 20-21 0.4145295540806231 2312 22-23 0.6583725564191241 2312 24-25 0.7150511644520776 2312 26-27 0.6627631021053375 2312 28-29 0.7651457431659239 2312 30-31 0.779091281423085 2312 32-33 0.7760962961940763 2312 34-35 0.40987309598772725 2312 36-37 0.7605660304127824 2312 38-39 0.7858112913461284 2312 40-41 0.5958603886325875 2312 42-43 0.6650048858117614 2312 44-45 0.7710691736996509 2312 46-47 0.7986770089747566 2312 48-49 -4.4732635194945125 2312 50-51 -0.7057413973200539 2312 52-53 0.5084890618045677 2312 54-55 0.8000812930577013 2312 56-57 0.9272945303045432 2312 58-59 0.9684750000607423 2312 60-61 0.9729523280881907 2312 62-63 0.7581149451227276 2312 64-65 1.021941033825719 2312 66-67 0.9437256931920501 2312 68-69 0.9194380821914976 2312 70-71 0.973683365499312 2312 72-73 1.0464013151896978 2312 74-75 0.7887385216838894 2312 76-77 0.9043971788062315 2312 78-79 0.6755258733018294 2312 80-81 0.8587160143262338 2312 82-83 0.8643135622279026 2312 84-85 0.8118831378663103 2312 86-87 0.8727117212114628 2312 88-89 0.9578458740835245 2312 90-91 0.7791649710967299 2312 92-93 0.9665801995148264 2312 94-95 1.109851394060133 2312 96-97 1.1415177863342656 2312 98-99 1.1118226410983247 2312 100 0.7805626849385732 2313 1 0.13150288894337336 2313 2 0.25642428811325146 2313 3 0.3340251281792561 2313 4 0.12391687160911147 2313 5 0.20220852835190328 2313 6 0.28979541677388454 2313 7 0.20550429161464479 2313 8 0.2723134616540719 2313 9 0.46672630613963406 2313 10-11 0.29333909986390694 2313 12-13 0.08691996982431505 2313 14-15 0.1497974909738815 2313 16-17 0.07735060127249227 2313 18-19 0.43463446416109974 2313 20-21 0.4461004401122963 2313 22-23 0.5777910529070382 2313 24-25 0.35117764443788957 2313 26-27 0.4512312633289852 2313 28-29 0.46393468846556374 2313 30-31 0.6025412683387756 2313 32-33 0.5274636705464459 2313 34-35 0.4713934718303534 2313 36-37 -0.3262397080493784 2313 38-39 0.2544747617730323 2313 40-41 0.4506052378174701 2313 42-43 0.5013604970275622 2313 44-45 0.5069863772621019 2313 46-47 0.45550891747746647 2313 48-49 -5.054684502355762 2313 50-51 -1.2566834731122114 2313 52-53 0.14472156861718588 2313 54-55 0.27656652534389536 2313 56-57 0.2996779864661434 2313 58-59 0.5325368690329668 2313 60-61 0.8454692382165163 2313 62-63 0.8897087605619873 2313 64-65 0.9416460771059079 2313 66-67 0.9588198952840656 2313 68-69 0.9707294535407058 2313 70-71 0.9874669945890773 2313 72-73 0.791553313761753 2313 74-75 0.8572280373122751 2313 76-77 0.8886438934260532 2313 78-79 0.818294889735391 2313 80-81 -0.16349804849149052 2313 82-83 0.24659765833132852 2313 84-85 0.6688821014585073 2313 86-87 0.8289570606332468 2313 88-89 0.8070688939201389 2313 90-91 0.8511184840504846 2313 92-93 0.9589662254551818 2313 94-95 0.8469224314676715 2313 96-97 0.8959079695882721 2313 98-99 1.0655819169411131 2313 100 1.1482621980435574 2314 1 0.02763461129982403 2314 2 0.1818627057901594 2314 3 0.23109253383722717 2314 4 0.1053838611688036 2314 5 0.23649155733197347 2314 6 0.3430476474878361 2314 7 0.17822522964334553 2314 8 0.30807095955243113 2314 9 0.47999331980144433 2314 10-11 0.3864878697932639 2314 12-13 0.3013152945431514 2314 14-15 0.3009241079606042 2314 16-17 0.5273449965040626 2314 18-19 0.5820082700472469 2314 20-21 0.6602395080661978 2314 22-23 0.41058359437608516 2314 24-25 0.4803678408250036 2314 26-27 0.552185625515925 2314 28-29 0.691778993071587 2314 30-31 0.5883110310686561 2314 32-33 0.5724992318562485 2314 34-35 0.4114677493059702 2314 36-37 0.5313274071226104 2314 38-39 0.5841656827590711 2314 40-41 0.3792904511014399 2314 42-43 0.4153641436984117 2314 44-45 0.6377806766959893 2314 46-47 0.7651034140512607 2314 48-49 -6.065159625370121 2314 50-51 -1.4105632342959709 2314 52-53 -0.6930079195888155 2314 54-55 -0.2885063969603081 2314 56-57 0.002464043302111918 2314 58-59 0.47015321760378725 2314 60-61 0.8033360044812881 2314 62-63 0.6790917216214609 2314 64-65 0.7947126658775758 2314 66-67 0.8980332635330655 2314 68-69 0.8920933309702264 2314 70-71 0.7104165600036296 2314 72-73 0.8341710711170336 2314 74-75 0.8813612056260283 2314 76-77 0.8077248732613889 2314 78-79 0.6063906585076353 2314 80-81 0.6895012822107702 2314 82-83 0.6940072560124513 2314 84-85 0.7147436132380278 2314 86-87 0.7684589298197011 2314 88-89 0.672780886222661 2314 90-91 0.8768366727311658 2314 92-93 0.9345179956924916 2314 94-95 0.9327162598363437 2314 96-97 0.9913202575243076 2314 98-99 1.0910722417127978 2314 100 -0.21127475527965345 2315 1 0.17585419291948057 2315 2 0.2774777471300922 2315 3 0.35427884451029 2315 4 0.1690696192662955 2315 5 0.2712440743421709 2315 6 0.36224487745529643 2315 7 0.40920122442808093 2315 8 0.28499757721041163 2315 9 0.455291305292576 2315 10-11 0.2898407195894137 2315 12-13 0.4945773436731784 2315 14-15 0.3660279153532997 2315 16-17 0.4175026891496927 2315 18-19 0.5034217163870878 2315 20-21 0.4346061449755254 2315 22-23 0.48330294767487914 2315 24-25 0.5540442596050639 2315 26-27 0.6304324467406062 2315 28-29 0.6922357962430326 2315 30-31 0.6554207268793277 2315 32-33 0.7242603699970758 2315 34-35 0.7489879621176669 2315 36-37 0.6732812040552147 2315 38-39 0.7271071309914134 2315 40-41 0.7474401667072783 2315 42-43 0.7727090099968805 2315 44-45 0.7729817120677964 2315 46-47 0.6240257973641761 2315 48-49 -4.796565729244133 2315 50-51 -0.9128045217775096 2315 52-53 0.25419473773632717 2315 54-55 0.28487339588715344 2315 56-57 0.5834588934643179 2315 58-59 0.7090310473337027 2315 60-61 0.6485631510245327 2315 62-63 0.38940825998828643 2315 64-65 0.641076080463229 2315 66-67 -0.1915800533767964 2315 68-69 0.5605545809177599 2315 70-71 0.5618434251862325 2315 72-73 0.6806693666554864 2315 74-75 0.8714137279215137 2315 76-77 0.7723357387172705 2315 78-79 0.700101731585093 2315 80-81 0.6160735309358856 2315 82-83 0.5413684208686043 2315 84-85 0.567943495704192 2315 86-87 0.63027778037074 2315 88-89 0.5904441135461198 2315 90-91 0.7479171164502851 2315 92-93 0.7736555223462105 2315 94-95 0.48873616836184297 2315 96-97 0.5773254037932603 2315 98-99 0.5428706232603275 2315 100 0.5288036368656321 2316 1 0.2665387563811237 2316 2 0.28748384736812227 2316 3 0.22194566581303832 2316 4 -0.04316451864675486 2316 5 0.1631581673773681 2316 6 0.19718019109768647 2316 7 0.2496599650916238 2316 8 0.3580576168861427 2316 9 0.4889387985817919 2316 10-11 0.4419928669871851 2316 12-13 0.3296153268087423 2316 14-15 0.47201652006720707 2316 16-17 0.4664515984471578 2316 18-19 0.19576154800531498 2316 20-21 0.4700879238823106 2316 22-23 0.6045263010164845 2316 24-25 0.6110429614909876 2316 26-27 0.6676826297304714 2316 28-29 0.6536407888353537 2316 30-31 0.6185959629526678 2316 32-33 0.5922986460546085 2316 34-35 0.5800854281009009 2316 36-37 0.4087040445070471 2316 38-39 0.5093244103474248 2316 40-41 0.16652980117314087 2316 42-43 0.09162234723814322 2316 44-45 0.38769896810749316 2316 46-47 0.43889329680890654 2316 48-49 -5.161435050815452 2316 50-51 -1.0358118924954667 2316 52-53 0.24948047309307242 2316 54-55 0.5666412662857638 2316 56-57 0.48962222371068975 2316 58-59 0.3628912700165685 2316 60-61 0.5498525171628259 2316 62-63 0.48081321976505365 2316 64-65 0.40886149543658235 2316 66-67 0.5222343173470279 2316 68-69 0.25227558034815445 2316 70-71 -0.16710507132615504 2316 72-73 -0.006469096657930606 2316 74-75 0.19043527012109962 2316 76-77 0.3108603913128576 2316 78-79 0.357187679655695 2316 80-81 0.2962227311518433 2316 82-83 0.14896021200695841 2316 84-85 0.3037958485273151 2316 86-87 0.4159638878903671 2316 88-89 0.28037695047805045 2316 90-91 0.24336593645492144 2316 92-93 0.30340154035193123 2316 94-95 0.5430690040502419 2316 96-97 0.5617527154501403 2316 98-99 0.6105799838786972 2316 100 -0.10320527238348376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 13.0 11 14.0 12 57.0 13 207.0 14 851.0 15 2348.0 16 5600.0 17 11366.0 18 20033.0 19 29812.0 20 43587.0 21 62035.0 22 77116.0 23 81906.0 24 84665.0 25 93121.0 26 109495.0 27 133612.0 28 165553.0 29 202283.0 30 247606.0 31 306215.0 32 384997.0 33 499747.0 34 687710.0 35 965671.0 36 1467954.0 37 1998761.0 38 1403458.0 39 154801.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61643757321656 20.297064106691423 14.710771999931588 23.375726320160435 2 15.53019960640381 20.287053585680763 40.01152543143261 24.17122137648282 3 18.777088332556687 25.550564779221098 29.822808081118524 25.849538807103695 4 13.061269243953763 16.890711996658396 37.557514974269594 32.49050378511824 5 13.772194134100731 37.57466673289641 34.111630235437005 14.541508897565853 6 31.409122479249728 36.83527328630753 17.29930582444737 14.456298409995366 7 27.995597257018833 32.27656247108549 21.562348258008704 18.165492013886972 8 26.559049064483986 35.12228243224641 19.367997482318657 18.950671020950953 9 26.418545795208182 16.004004007037427 20.423022542615897 37.1544276551385 10-11 25.665264634344766 26.139523941009994 26.94460357158684 21.250607853058398 12-13 26.005141983513514 23.632405804927657 27.67714425392947 22.685307957629362 14-15 23.35839087470313 24.969401330952824 25.382502301257993 26.289705493086053 16-17 22.517755795378307 27.967092305786835 26.275238622879993 23.239913275954866 18-19 22.29154667994718 27.000615544045488 28.65852629145751 22.049311484549822 20-21 23.358461216471056 26.036496772178598 28.470581665818596 22.13446034553175 22-23 23.40607154463556 25.789817761458938 28.295179707856033 22.50893098604947 24-25 22.96832837243882 26.049728345708967 28.511910205013635 22.470033076838583 26-27 22.81478057781857 26.223525865394336 28.274978263044513 22.686715293742576 28-29 22.51467342299932 26.28972775979177 28.564346406629245 22.63125241057967 30-31 22.96959532106177 26.049060499305927 28.420065090347407 22.5612790892849 32-33 22.99049428104332 26.369659104000988 28.040693942755073 22.599152672200617 34-35 23.03651473965559 26.27925195860917 28.349107485544362 22.33512581619088 36-37 23.031621988293153 26.117040518047023 28.140323452503807 22.711014041156016 38-39 22.99407109330152 26.111175483915872 28.366396270218647 22.528357152563963 40-41 22.831654152739482 25.85579715701164 28.600042758946625 22.71250593130225 42-43 22.78187643292022 25.95151530171701 28.290843038820885 22.975765226541885 44-45 22.83385982517325 26.312813211056195 28.045403914553773 22.80792304921678 46-47 22.87533384501301 26.23292190614767 27.738072967008133 23.153671281831183 48-49 23.3036737606923 26.28619740598401 27.546649834128083 22.863478999195614 50-51 23.053118805788827 26.371059368204396 27.377573190255323 23.198248635751455 52-53 22.83796207074716 26.596462106222322 27.331661157057447 23.233914665973074 54-55 22.85531334355112 26.538694873427865 27.26782553744165 23.338166245579366 56-57 23.11294206074367 26.411021556798413 27.10672770273031 23.369308679727606 58-59 22.791765315005847 26.4499682463821 27.432674537754338 23.325591900857713 60-61 22.87496291321229 26.708601770068576 27.198074317079573 23.21836099963956 62-63 22.934876124683715 26.547873940509266 27.077388650961804 23.43986128384522 64-65 22.59277168682018 26.41188824075219 27.27490433767522 23.72043573475241 66-67 22.80750912439031 26.327746075739544 27.16997792112147 23.694766878748673 68-69 22.61255484604193 26.40859617243676 27.211842020017606 23.767006961503704 70-71 22.61436916601688 26.149331241456398 27.333859802498832 23.902439790027888 72-73 22.861357794256516 26.416577150667877 27.087043322279676 23.635021732795934 74-75 22.841086302000996 26.585364692187053 26.994921183482106 23.578627822329846 76-77 22.702948099084928 26.501466313588985 26.895288309164094 23.900297278161997 78-79 22.606851893679444 26.456105600631354 27.025372329412896 23.91167017627631 80-81 22.49836221234418 26.74643735529178 26.968452478834298 23.78674795352974 82-83 22.80068337992742 26.364492384221 27.30956223205695 23.52526200379463 84-85 22.884806120645305 26.449306776216886 26.835846673427504 23.830040429710312 86-87 22.42561322769704 26.67635396857809 27.118495647776935 23.779537155947935 88-89 22.604651782092677 26.228224168616737 27.117162300124914 24.049961749165675 90-91 22.615821053390395 26.21756362150925 27.160383115602514 24.006232209497835 92-93 22.643110033222708 26.372319866174053 27.02875404893617 23.955816051667068 94-95 22.192464667917168 26.203890371084814 27.270086592661247 24.333558368336774 96-97 22.32671636499484 26.126675327295136 27.23117601147324 24.315432296236782 98-99 22.126673699362172 26.300637829368075 27.12679465104573 24.445893820224022 100 22.159102780153056 26.152028148701927 26.969120368220324 24.719748702924694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1957.0 1 1407.0 2 1297.0 3 1705.5 4 1318.5 5 1131.5 6 1845.5 7 3623.0 8 5915.5 9 7258.0 10 6688.0 11 5622.5 12 5736.5 13 6481.0 14 7480.5 15 8552.0 16 9584.0 17 10683.5 18 11542.5 19 12482.0 20 13641.5 21 14834.5 22 16546.0 23 18846.5 24 21704.0 25 25363.5 26 30594.5 27 37111.0 28 44913.0 29 56669.5 30 72654.5 31 89765.5 32 112322.0 33 138162.0 34 162768.0 35 186495.0 36 213914.0 37 248248.0 38 264539.0 39 262241.5 40 258592.0 41 257042.5 42 262980.0 43 278322.0 44 298662.5 45 325990.5 46 351351.5 47 367573.0 48 381926.5 49 387931.5 50 382623.0 51 377945.0 52 371425.0 53 361957.5 54 352662.5 55 329077.0 56 297808.0 57 261286.0 58 227190.5 59 202025.5 60 170732.0 61 137713.5 62 110517.0 63 87507.5 64 66175.0 65 47431.0 66 34736.5 67 26738.5 68 20135.5 69 14707.5 70 11522.5 71 9049.5 72 7033.5 73 5587.5 74 4381.5 75 3330.0 76 2395.5 77 1486.0 78 878.5 79 558.0 80 365.5 81 213.5 82 135.0 83 88.0 84 53.0 85 33.5 86 20.5 87 14.5 88 9.0 89 5.5 90 3.5 91 3.0 92 2.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028147501565643905 2 0.0018613495845024033 3 5.410899954948847E-5 4 0.02084278662646296 5 4.97802795855294E-4 6 5.410899954948847E-5 7 4.3287199639590775E-5 8 0.003885026167653272 9 0.00933921332224171 10-11 0.0010010164916655366 12-13 1.028070991440281E-4 14-15 9.739619918907924E-5 16-17 3.5170849707167505E-4 18-19 1.0821799909897694E-5 20-21 9.739619918907924E-5 22-23 2.8136679765734004E-4 24-25 2.1643599819795388E-5 26-27 8.11634993242327E-5 28-29 9.847837918006903E-4 30-31 0.0023807959801774928 32-33 0.009236406223097682 34-35 0.01788843525106089 36-37 0.020658816027994697 38-39 0.020436969129841796 40-41 0.004712893860760446 42-43 0.005589459653462159 44-45 0.008992915725124983 46-47 0.011931034400662208 48-49 1.1976594178282876 50-51 0.017726108252412425 52-53 0.018921917142456116 54-55 0.009052435624629422 56-57 0.0077321760356219025 58-59 0.007737586935576851 60-61 0.004940151658868297 62-63 0.007916146634090163 64-65 0.01871630294416806 66-67 0.028055516266409772 68-69 0.028012229066770183 70-71 0.025241848289836374 72-73 0.022022362816641806 74-75 0.009263460722872426 76-77 0.013857314784623997 78-79 0.007986488333504498 80-81 0.002656751877879884 82-83 0.013386566488543446 84-85 0.02276906701042475 86-87 0.022211744315065014 88-89 0.03138321973870332 90-91 0.01979307203520288 92-93 0.012369317297013065 94-95 0.006092673349272402 96-97 0.0034954413708969552 98-99 0.014939494775613767 100 0.03348264892122347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9240607.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.7923017852968 #Duplication Level Percentage of deduplicated Percentage of total 1 70.43808650107836 36.4815063324269 2 15.445903292501189 15.999577693434622 3 5.9701480368191016 9.276231264774964 4 2.8458284036079373 5.895680140353268 5 1.530103708692321 3.962379652169728 6 0.940070567058871 2.9213051105152887 7 0.6105086790388017 2.213375482312436 8 0.43889418205529696 1.8185071943055149 9 0.3165134822682018 1.4753665613474904 >10 1.361670610846744 11.942579359369669 >50 0.06524176103607572 2.302026258752496 >100 0.03434432559844976 3.195776026618724 >500 0.001967992041619316 0.7190174689651895 >1k 5.93508251628959E-4 0.51934617158299 >5k 3.1237276369187795E-5 0.1107903984505698 >10k+ 9.371182910756338E-5 1.1665348846203103 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30715 0.33239158423250764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25912 0.2804144792652691 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15477 0.1674889972054866 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0821799909897694E-5 0.0 5 0.0 0.0 0.0 1.0821799909897694E-5 0.0 6 0.0 0.0 0.0 1.0821799909897694E-5 3.246539972969308E-5 7 0.0 0.0 0.0 1.0821799909897694E-5 3.246539972969308E-5 8 0.0 0.0 0.0 2.1643599819795388E-5 3.246539972969308E-5 9 1.0821799909897694E-5 0.0 0.0 4.3287199639590775E-5 3.246539972969308E-5 10-11 1.0821799909897694E-5 0.0 0.0 4.3287199639590775E-5 3.246539972969308E-5 12-13 1.0821799909897694E-5 0.0 0.0 5.9519899504437315E-5 4.869809959453962E-5 14-15 1.0821799909897694E-5 0.0 0.0 7.575259936928386E-5 1.1903979900887463E-4 16-17 1.0821799909897694E-5 0.0 0.0 9.739619918907924E-5 1.731487985583631E-4 18-19 1.0821799909897694E-5 2.1643599819795388E-5 0.0 9.739619918907924E-5 1.8938149842320963E-4 20-21 1.0821799909897694E-5 2.7054499774744235E-5 0.0 1.8397059846826078E-4 1.9479239837815848E-4 22-23 1.0821799909897694E-5 3.246539972969308E-5 0.0 3.571193970266239E-4 2.2184689815290274E-4 24-25 1.0821799909897694E-5 3.246539972969308E-5 0.0 8.008131933324293E-4 2.4890139792764694E-4 26-27 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.0017639533853133242 2.7054499774744234E-4 28-29 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.004399061663373413 2.759558977023912E-4 30-31 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.01299698169178713 2.867776976122889E-4 32-33 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.034348392914015285 3.246539972969308E-4 34-35 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.06717632294068993 3.354757972068285E-4 36-37 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.12022478609900844 3.462975971167262E-4 38-39 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.19695134746018308 3.679411969365216E-4 40-41 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.27850984248112703 3.949956967112658E-4 42-43 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.362292217383555 4.0040659666621466E-4 44-45 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.464688088131007 4.1122839657611236E-4 46-47 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.588548998999741 4.274610964409589E-4 48-49 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.725011895863551 4.4369379630580547E-4 50-51 1.0821799909897694E-5 3.246539972969308E-5 0.0 0.8680165707728941 4.4369379630580547E-4 52-53 1.0821799909897694E-5 3.246539972969308E-5 0.0 1.0096306443938152 4.869809959453962E-4 54-55 1.0821799909897694E-5 3.787629968464193E-5 0.0 1.164815255101748 5.410899954948847E-4 56-57 1.0821799909897694E-5 4.3287199639590775E-5 0.0 1.348028327576316 5.519117954047824E-4 58-59 1.0821799909897694E-5 5.410899954948847E-5 0.0 1.5568241350378822 5.573226953597312E-4 60-61 2.1643599819795388E-5 5.410899954948847E-5 0.0 1.7713230310519643 5.627335953146801E-4 62-63 2.1643599819795388E-5 5.410899954948847E-5 0.0 1.9871854738546937 5.843771951344755E-4 64-65 2.1643599819795388E-5 5.410899954948847E-5 0.0 2.2099630467998477 5.843771951344755E-4 66-67 2.1643599819795388E-5 5.410899954948847E-5 0.0 2.4603578531150605 5.897880950894243E-4 68-69 2.1643599819795388E-5 5.410899954948847E-5 0.0 2.7376989412059185 6.00609894999322E-4 70-71 2.1643599819795388E-5 5.410899954948847E-5 0.0 3.036055964721798 6.276643947740663E-4 72-73 2.1643599819795388E-5 5.410899954948847E-5 0.0 3.3341099778402006 7.467041937829409E-4 74-75 2.1643599819795388E-5 5.410899954948847E-5 0.0 3.6313198905656305 7.683477936027363E-4 76-77 2.1643599819795388E-5 5.410899954948847E-5 0.0 3.957802772047334 7.791695935126339E-4 78-79 2.1643599819795388E-5 5.410899954948847E-5 0.0 4.3116485746012145 8.008131933324293E-4 80-81 2.1643599819795388E-5 5.410899954948847E-5 0.0 4.68871254886178 8.386894930170713E-4 82-83 2.1643599819795388E-5 5.410899954948847E-5 0.0 5.075207721743821 8.927984925665598E-4 84-85 2.1643599819795388E-5 5.410899954948847E-5 0.0 5.455382963478481 9.144420923863552E-4 86-87 2.1643599819795388E-5 5.410899954948847E-5 0.0 5.847840948110877 9.198529923413039E-4 88 2.1643599819795388E-5 5.410899954948847E-5 0.0 6.156630186739897 9.414965921610993E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17645 0.0 53.51416 1 GTATCAA 31260 0.0 40.91098 1 CAACGCA 39490 0.0 31.563717 5 ATCAACG 39550 0.0 31.4991 3 TATCAAC 40645 0.0 31.099045 2 TCAACGC 41785 0.0 29.91566 4 AACGCAG 42430 0.0 29.763458 6 GTACTAG 3765 0.0 28.669838 1 GTGGTAT 7045 0.0 27.978903 1 ACGCAGA 47905 0.0 25.970123 7 CGCAGAG 49375 0.0 25.187437 8 GTACATG 43935 0.0 24.002409 1 TACATGG 44485 0.0 23.425646 2 GCAGAGT 53365 0.0 23.057331 9 ACATGGG 45055 0.0 22.44318 3 TGGTATC 8720 0.0 21.845707 2 GAGTACT 28070 0.0 21.339087 12-13 TAGTACT 5285 0.0 21.308409 4 CAGAGTA 52350 0.0 20.402264 10-11 CATGGGG 30650 0.0 20.162302 4 >>END_MODULE