FastQCFastQC Report
Mon 6 Jun 2016
ERR1211276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499120
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516680.6079217613117593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395580.4654364216530652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235120.2766404051242952No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG137540.1618285187172319No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT134000.15766338162068544No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA110710.13026054462109019No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT101320.11921234198364065No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC91700.10789352309415563No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG89870.10574035900187313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89090.10482261692975274No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC88860.10455200067771722No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT86660.10196349739737762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198250.054.7354431
GTATCAA405800.043.477971
TACCTGG21850.041.1157762
ACCTGGG22950.039.311583
CAACGCA484850.035.305595
ATCAACG489600.034.963893
GTACACG2750.034.20831
GCATCGT35200.033.6406948
AACGCAG522550.033.1187026
TCAACGC528150.032.5801284
TATCAAC535150.032.344382
TAGGCAT54050.032.3399245
GGTACTC17200.030.32473
CATGGGT50650.029.8722674
ACGCAGA579200.029.5154177
CGCAGAG581100.029.3375268
GTACTAG47550.028.9835341
GTGGTAC18200.028.9454881
TCTCTGC36400.028.6588448
TCAAGAC7800.028.314263