FastQCFastQC Report
Mon 6 Jun 2016
ERR1211276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499120
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433810.5104175491109667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354550.4171608354747315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221740.2608975988102298No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG139000.16354634362145729No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT125220.1473329003473301No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA109830.12922514330895432No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT101470.11938883084366382No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG88730.10439904366569715No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA87670.10315185572153353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196400.055.9879461
GTATCAA390350.041.214531
ACCTGGG22250.037.116953
TACCTGG22800.036.424392
CAACGCA479900.032.522345
TACCCCG3200.032.2516825
ATCAACG484350.032.2413833
TATCACG2250.031.27972
TAGGCAT55350.031.1048535
CATGGGT46600.030.7118574
AACGCAG516400.030.550466
TCAACGC518050.030.2619534
TCTCTGC39000.029.830588
TATCAAC533500.029.6468242
GTGGTAC19200.028.8432711
GGTACTC19150.027.9335213
ATGGGTA36650.027.9037275
GTACTAG48100.027.612461
AGGGGTC29750.027.1219675
TCACGCA2600.027.0713624