##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211276_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8499120 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05229812027598 33.0 31.0 34.0 30.0 34.0 2 32.20562093487326 33.0 31.0 34.0 30.0 34.0 3 32.250330151827484 34.0 31.0 34.0 30.0 34.0 4 35.913007699620664 37.0 35.0 37.0 35.0 37.0 5 35.82741107314639 37.0 35.0 37.0 35.0 37.0 6 35.85015813401858 37.0 35.0 37.0 35.0 37.0 7 35.772896958743964 37.0 35.0 37.0 33.0 37.0 8 35.75662633307919 37.0 35.0 37.0 33.0 37.0 9 37.34173855646232 39.0 37.0 39.0 34.0 39.0 10-11 37.29567231666337 39.0 37.0 39.0 34.0 39.0 12-13 37.244681096395865 39.0 37.0 39.0 33.0 39.0 14-15 38.41374718794417 40.0 38.0 41.0 33.5 41.0 16-17 38.41345180442211 40.0 38.0 41.0 33.5 41.0 18-19 38.48724267924209 40.0 38.0 41.0 34.0 41.0 20-21 38.48606355716827 40.0 38.0 41.0 34.0 41.0 22-23 38.36491748557498 40.0 38.0 41.0 34.0 41.0 24-25 38.28021583410988 40.0 38.0 41.0 34.0 41.0 26-27 38.16288109827841 40.0 38.0 41.0 33.0 41.0 28-29 38.09128892167659 40.0 37.0 41.0 33.0 41.0 30-31 38.06620620723086 40.0 37.0 41.0 33.0 41.0 32-33 37.96826700881974 40.0 37.0 41.0 33.0 41.0 34-35 37.845580365967294 40.0 37.0 41.0 33.0 41.0 36-37 37.696410216587125 40.0 37.0 41.0 33.0 41.0 38-39 37.58051074699498 40.0 36.0 41.0 33.0 41.0 40-41 37.48069294232815 40.0 36.0 41.0 32.5 41.0 42-43 37.36803839691639 40.0 36.0 41.0 32.0 41.0 44-45 37.18059834429917 40.0 36.0 41.0 31.0 41.0 46-47 36.89276030930261 39.0 36.0 41.0 30.5 41.0 48-49 35.31349086728979 39.0 35.0 40.5 23.0 41.0 50-51 36.04113614115344 39.0 35.0 40.0 28.0 41.0 52-53 36.1513584347556 39.0 35.0 40.0 29.0 41.0 54-55 36.00043275068478 39.0 35.0 40.0 28.5 41.0 56-57 35.80021008057305 38.0 35.0 40.0 28.0 41.0 58-59 35.583739198881766 38.0 34.5 40.0 28.0 41.0 60-61 35.365065442069294 38.0 34.0 40.0 27.5 41.0 62-63 35.07056242293319 37.5 34.0 40.0 27.0 41.0 64-65 34.76920640019202 37.0 34.0 40.0 26.0 41.0 66-67 34.46417387917808 36.5 33.5 39.0 26.0 41.0 68-69 34.127770992761604 36.0 33.0 39.0 26.0 40.0 70-71 33.81606972251245 36.0 33.0 39.0 26.0 40.0 72-73 33.45573800581707 35.0 32.5 38.0 25.0 40.0 74-75 33.078286987358695 35.0 32.0 37.5 25.0 39.5 76-77 32.123251289545266 34.0 31.0 36.0 23.5 39.0 78-79 32.56197941669255 35.0 32.0 36.0 25.0 39.0 80-81 32.41997459737008 35.0 32.0 36.0 25.0 38.5 82-83 32.130835427667805 35.0 31.5 36.0 24.0 37.0 84-85 31.79694815463248 34.0 31.0 35.0 24.0 37.0 86-87 31.53307348290176 34.0 31.0 35.0 23.0 36.5 88-89 31.282061083971044 34.0 31.0 35.0 23.0 36.0 90-91 31.045989702463313 34.0 31.0 35.0 21.0 36.0 92-93 30.832892287672138 34.0 30.5 35.0 20.0 35.0 94-95 30.62460260591685 34.0 30.0 35.0 20.0 35.0 96-97 30.439677931362304 34.0 30.0 35.0 20.0 35.0 98-99 30.232839282184507 34.0 29.0 35.0 19.0 35.0 100 27.74245415996009 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3269240006051284 1101 2 -0.2911018308213116 1101 3 -0.2972302333938899 1101 4 -0.17679824402797806 1101 5 -0.19954695591820126 1101 6 -0.22851883084249636 1101 7 -0.19792741217060694 1101 8 -0.2490946798401268 1101 9 -0.4114873898045843 1101 10-11 -0.3882870597686292 1101 12-13 -0.4255164805024023 1101 14-15 -0.509941805149964 1101 16-17 -0.4807166436647847 1101 18-19 -0.5005844841560005 1101 20-21 -0.5822993463982726 1101 22-23 -0.6882785569854377 1101 24-25 -0.6066981575860879 1101 26-27 -0.6190826307046251 1101 28-29 -0.6370637591550405 1101 30-31 -0.5351327867181865 1101 32-33 -0.4786424801520468 1101 34-35 -0.5277057002033629 1101 36-37 -0.5520012429104 1101 38-39 -0.5470612228214904 1101 40-41 -0.5061286507697673 1101 42-43 -0.5858956722435948 1101 44-45 -0.49869070875163857 1101 46-47 -0.4842983160882923 1101 48-49 0.7568420141934169 1101 50-51 -0.16826632388261942 1101 52-53 -0.470125174020545 1101 54-55 -0.5057942435615388 1101 56-57 -0.5192708334474858 1101 58-59 -0.6097575959143882 1101 60-61 -0.6142841416059923 1101 62-63 -0.5996889203800251 1101 64-65 -0.6386609953586984 1101 66-67 -0.6333822398112687 1101 68-69 -0.6327212534935143 1101 70-71 -0.6026546000873978 1101 72-73 -0.7193495215316403 1101 74-75 -0.7441131087752453 1101 76-77 -0.6490948280703037 1101 78-79 -0.584921993670342 1101 80-81 -0.41963636336232213 1101 82-83 -0.4460417166288764 1101 84-85 -0.4944102405461237 1101 86-87 -0.4464768017819587 1101 88-89 -0.5117480286816161 1101 90-91 -0.4891406550730508 1101 92-93 -0.4768781172398029 1101 94-95 -0.5386359471441651 1101 96-97 -0.6841352749539809 1101 98-99 -0.8564611530093202 1101 100 -0.8829713779002084 1102 1 -0.28302684235203657 1102 2 -0.23698267840856957 1102 3 -0.29676739565997323 1102 4 -0.20972169186479306 1102 5 -0.22244110007520135 1102 6 -0.2831622602121371 1102 7 -0.27847861847009625 1102 8 -0.27356921687290736 1102 9 -0.33098061235337894 1102 10-11 -0.3676154584055027 1102 12-13 -0.46025675384026243 1102 14-15 -0.5508775200482745 1102 16-17 -0.5001282362715358 1102 18-19 -0.5590298977395136 1102 20-21 -0.6245220417560162 1102 22-23 -0.6722461464632801 1102 24-25 -0.612619993557523 1102 26-27 -0.6697711893263829 1102 28-29 -0.5530308617522763 1102 30-31 -0.6667964293274267 1102 32-33 -0.5888858167059396 1102 34-35 -0.5153187056408726 1102 36-37 -0.5867850101878247 1102 38-39 -0.5404231971250013 1102 40-41 -0.4947938587471583 1102 42-43 -0.5107660181914255 1102 44-45 -0.4784001530192157 1102 46-47 -0.5037431765533356 1102 48-49 0.6785939679499506 1102 50-51 -0.27049364546657984 1102 52-53 -0.4207128981858048 1102 54-55 -0.5605229978310717 1102 56-57 -0.5562598435705937 1102 58-59 -0.692951350684389 1102 60-61 -0.6048018588130404 1102 62-63 -0.6007001358594977 1102 64-65 -0.6331936822249844 1102 66-67 -0.6206164086501929 1102 68-69 -0.6946165299159972 1102 70-71 -0.6247704534140794 1102 72-73 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1105 70-71 -0.8026107105809004 1105 72-73 -0.6705058158250239 1105 74-75 -0.6715285579172345 1105 76-77 -0.7487346102834707 1105 78-79 -0.5686144996863689 1105 80-81 -0.5438748576297172 1105 82-83 -0.5855461484193327 1105 84-85 -0.5691778124957843 1105 86-87 -0.5343975406299712 1105 88-89 -0.5963450037808755 1105 90-91 -0.6429701710776712 1105 92-93 -0.648695289637093 1105 94-95 -0.5782826823689078 1105 96-97 -0.586551600604384 1105 98-99 -0.6063114976121966 1105 100 -0.6281731939445407 1106 1 -0.32349084996912936 1106 2 -0.30233262584115295 1106 3 -0.3212170515088566 1106 4 -0.2125711886164865 1106 5 -0.22218533711609467 1106 6 -0.32043025031162387 1106 7 -0.27863611434633384 1106 8 -0.3703281287798035 1106 9 -0.47157922498727345 1106 10-11 -0.47656580433696405 1106 12-13 -0.48587059613144135 1106 14-15 -0.6119718214874297 1106 16-17 -0.5330628767758867 1106 18-19 -0.5876427260795012 1106 20-21 -0.5937165545340406 1106 22-23 -0.6269458915407569 1106 24-25 -0.6071287484036816 1106 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0.19103439116702248 2311 9 0.48923020525265315 2311 10-11 0.3544529603967632 2311 12-13 0.42785581504610093 2311 14-15 0.439100114869035 2311 16-17 0.4541514574690737 2311 18-19 0.5821648576085678 2311 20-21 0.6511320532668847 2311 22-23 0.6980450087749688 2311 24-25 0.5954485211723224 2311 26-27 0.6342214709142269 2311 28-29 0.6936423638424856 2311 30-31 0.5803919998640268 2311 32-33 0.6229795756977055 2311 34-35 0.7172335911368677 2311 36-37 0.718631524510009 2311 38-39 0.7684716152801414 2311 40-41 0.7360767701935629 2311 42-43 0.7702208630628959 2311 44-45 0.7599640341659963 2311 46-47 0.8010520663148313 2311 48-49 -6.01190594539365 2311 50-51 -1.0516006004003984 2311 52-53 0.36698478052428385 2311 54-55 0.7745571957729354 2311 56-57 0.8577735188352236 2311 58-59 0.8546364232163697 2311 60-61 0.7328071758401435 2311 62-63 0.6558574819564313 2311 64-65 0.7648813004317603 2311 66-67 0.867140085567847 2311 68-69 0.8695379906572214 2311 70-71 0.8815130900191122 2311 72-73 0.3937070744824922 2311 74-75 0.6634404973667856 2311 76-77 0.7608578055457826 2311 78-79 0.6498425627549764 2311 80-81 0.673006601429428 2311 82-83 0.734147065747571 2311 84-85 0.7284336618409917 2311 86-87 0.7711466933107864 2311 88-89 0.8250910003674932 2311 90-91 0.8689820640443422 2311 92-93 0.8634588225823414 2311 94-95 0.8504842203599701 2311 96-97 0.868630147484101 2311 98-99 0.8955506797421862 2311 100 1.0625352066906615 2312 1 0.11156785778776879 2312 2 0.24128302753150166 2312 3 0.22691462029570175 2312 4 0.03504980518324885 2312 5 0.21179721317115252 2312 6 0.14699363388633202 2312 7 0.2600459058470719 2312 8 0.3683881909327482 2312 9 0.30931174591907507 2312 10-11 0.2350990759522844 2312 12-13 0.24787716737699128 2312 14-15 0.3360157601557887 2312 16-17 0.4424320275397804 2312 18-19 0.3030606426816931 2312 20-21 0.35220913390845965 2312 22-23 0.6040541291757222 2312 24-25 0.7106771939544885 2312 26-27 0.6260878544697164 2312 28-29 0.692514697326267 2312 30-31 0.7273720259152014 2312 32-33 0.7137298046209253 2312 34-35 0.3443571947328863 2312 36-37 0.6649849553973297 2312 38-39 0.7390923465781043 2312 40-41 0.5029189192962633 2312 42-43 0.5369284670219869 2312 44-45 0.6155913668099018 2312 46-47 0.691872307416844 2312 48-49 -5.0091875634332155 2312 50-51 -0.8791250168018436 2312 52-53 0.40282791671568674 2312 54-55 0.6731710099783186 2312 56-57 0.7229052953153925 2312 58-59 0.7713606723634285 2312 60-61 0.8002660962634138 2312 62-63 0.5489143769522755 2312 64-65 0.8049209610857773 2312 66-67 0.7462376590232083 2312 68-69 0.6550736405542636 2312 70-71 0.6733708396616507 2312 72-73 0.8272744274437756 2312 74-75 0.4755409263781729 2312 76-77 0.681628260517023 2312 78-79 0.30468855394894234 2312 80-81 0.5449965550968585 2312 82-83 0.5378201592370715 2312 84-85 0.6081798580073716 2312 86-87 0.7427579170655498 2312 88-89 0.7116301766091766 2312 90-91 0.5466059226451989 2312 92-93 0.7139236010602126 2312 94-95 0.8435346122482983 2312 96-97 0.8402681308344846 2312 98-99 0.9052114195412351 2312 100 0.5642035720059084 2313 1 0.05206679470872899 2313 2 0.1694561881508534 2313 3 0.27233890071805433 2313 4 0.11775699346923574 2313 5 0.17979429958904092 2313 6 0.2896714394557236 2313 7 0.189179985771446 2313 8 0.2731955852470662 2313 9 0.4561960572270891 2313 10-11 0.26482711736344555 2313 12-13 0.08913341570552547 2313 14-15 0.14507578869108073 2313 16-17 0.07882895264544487 2313 18-19 0.40498873993004025 2313 20-21 0.3920348871646908 2313 22-23 0.48775854872852165 2313 24-25 0.2996859900636295 2313 26-27 0.45509123612897895 2313 28-29 0.347983822698005 2313 30-31 0.5254383330884096 2313 32-33 0.4537060347331021 2313 34-35 0.4227835822301458 2313 36-37 -0.2731310629378356 2313 38-39 0.14599799655440648 2313 40-41 0.37485420939654546 2313 42-43 0.4283236048308936 2313 44-45 0.49086105563159066 2313 46-47 0.35608566153971566 2313 48-49 -5.1650715863910435 2313 50-51 -1.3326327948984513 2313 52-53 0.08276936906801069 2313 54-55 0.2360150053054184 2313 56-57 0.27106829332928584 2313 58-59 0.3455904946294197 2313 60-61 0.6138955129631753 2313 62-63 0.7581197611493806 2313 64-65 0.7809436541212378 2313 66-67 0.8267506258598161 2313 68-69 0.8535600419779357 2313 70-71 0.8037571539764983 2313 72-73 0.5820231140057288 2313 74-75 0.6934245406107991 2313 76-77 0.7389289351507884 2313 78-79 0.6636781822370423 2313 80-81 -0.2748096093222614 2313 82-83 0.11904235216366743 2313 84-85 0.5635086303153578 2313 86-87 0.698631363409806 2313 88-89 0.6430057331279997 2313 90-91 0.7214201001159246 2313 92-93 0.8240302130016168 2313 94-95 0.7168817347113041 2313 96-97 0.6442211913706082 2313 98-99 0.8025271105943617 2313 100 0.9805574424238479 2314 1 0.05617736620821745 2314 2 0.19779005400650362 2314 3 0.20875362374009399 2314 4 0.05375918686765857 2314 5 0.21151472092135748 2314 6 0.2964643448241162 2314 7 0.13644647906335194 2314 8 0.2743727707545176 2314 9 0.39447268146755476 2314 10-11 0.2966591901191791 2314 12-13 0.1582168464962166 2314 14-15 0.20016580969273434 2314 16-17 0.4363421686375659 2314 18-19 0.46279101311613147 2314 20-21 0.5184691682228788 2314 22-23 0.2937885674484164 2314 24-25 0.37788577941230983 2314 26-27 0.4387821579918807 2314 28-29 0.5503706551110099 2314 30-31 0.4723038065753613 2314 32-33 0.4635734372944995 2314 34-35 0.2853459196964607 2314 36-37 0.4085601057318442 2314 38-39 0.5144288886356279 2314 40-41 0.27402501403364 2314 42-43 0.30742675213671333 2314 44-45 0.4766598199407568 2314 46-47 0.5561293596945234 2314 48-49 -6.194498833497143 2314 50-51 -1.524872480514091 2314 52-53 -0.9321936321717104 2314 54-55 -0.42966837559633575 2314 56-57 -0.0851435609472162 2314 58-59 0.32802618678931594 2314 60-61 0.5236242680636636 2314 62-63 0.4892147294564708 2314 64-65 0.62019486323954 2314 66-67 0.6202371473572725 2314 68-69 0.6871723003613894 2314 70-71 0.4897221130600755 2314 72-73 0.6027181028223438 2314 74-75 0.6542544793204499 2314 76-77 0.5879589356528143 2314 78-79 0.37329369597228634 2314 80-81 0.4526226915113085 2314 82-83 0.3517833378839086 2314 84-85 0.38489003194872495 2314 86-87 0.4680768516721834 2314 88-89 0.3796291908844296 2314 90-91 0.5877352721713294 2314 92-93 0.6839837578485266 2314 94-95 0.7472020128808516 2314 96-97 0.7186924692518808 2314 98-99 0.7597268401373221 2314 100 -0.6011748205231413 2315 1 0.10530058983180623 2315 2 0.20829905355642353 2315 3 0.3049123983190114 2315 4 0.11614764200053429 2315 5 0.17942564972715758 2315 6 0.2895127125242709 2315 7 0.31825431875962806 2315 8 0.2326674139032292 2315 9 0.4408781858384785 2315 10-11 0.22674416299346234 2315 12-13 0.42629252804689344 2315 14-15 0.2537259810938082 2315 16-17 0.36517015420648136 2315 18-19 0.43247255030201615 2315 20-21 0.31918782952828906 2315 22-23 0.4269405715999781 2315 24-25 0.4651818395446625 2315 26-27 0.5210657982200217 2315 28-29 0.6124267828553513 2315 30-31 0.6026004573541925 2315 32-33 0.6168556960345768 2315 34-35 0.6117498054688397 2315 36-37 0.5474744627009827 2315 38-39 0.6032088415826777 2315 40-41 0.5775163240052024 2315 42-43 0.6242190554166385 2315 44-45 0.6082416770128347 2315 46-47 0.4669928536240704 2315 48-49 -4.865568194704444 2315 50-51 -1.0068097548509343 2315 52-53 0.13260586038344968 2315 54-55 0.028848558636028088 2315 56-57 0.41141922434852773 2315 58-59 0.5123555439075318 2315 60-61 0.4093864398537619 2315 62-63 0.14227034890406998 2315 64-65 0.29390305476801615 2315 66-67 -0.3559784166036337 2315 68-69 0.37860796177896816 2315 70-71 0.36510360182997914 2315 72-73 0.4206049123800213 2315 74-75 0.5959147390345265 2315 76-77 0.5470533830078992 2315 78-79 0.47004001117588956 2315 80-81 0.34763270481505515 2315 82-83 0.33671945648458745 2315 84-85 0.38806227213170175 2315 86-87 0.4455878269062019 2315 88-89 0.4373593832846865 2315 90-91 0.47718987190184947 2315 92-93 0.5377378771108461 2315 94-95 0.20319095174751922 2315 96-97 0.36474489906646923 2315 98-99 0.2332899967168558 2315 100 0.27800993114266603 2316 1 0.27488909033671405 2316 2 0.25038400300432784 2316 3 0.1987476878747927 2316 4 -0.012835468962784091 2316 5 0.18859264589893598 2316 6 0.11809997039512155 2316 7 0.23635927152691494 2316 8 0.3348027659631825 2316 9 0.48786228784988594 2316 10-11 0.42137650232880475 2316 12-13 0.3683876680674487 2316 14-15 0.4742234935909124 2316 16-17 0.4331589980125159 2316 18-19 0.1701076610559582 2316 20-21 0.4605248694092836 2316 22-23 0.5829291271697343 2316 24-25 0.5840699815221839 2316 26-27 0.5972175787544103 2316 28-29 0.6307878060404875 2316 30-31 0.5717385315683785 2316 32-33 0.49662721068243343 2316 34-35 0.5029023224856601 2316 36-37 0.41967294168352254 2316 38-39 0.5540225081537002 2316 40-41 0.12991096455857587 2316 42-43 0.15274773239326578 2316 44-45 0.33801466661198987 2316 46-47 0.3155805405716592 2316 48-49 -5.054033400106562 2316 50-51 -1.0381404519446988 2316 52-53 0.1822937914776972 2316 54-55 0.46295105176062634 2316 56-57 0.2927910214321727 2316 58-59 0.2412486083487977 2316 60-61 0.42233900035432725 2316 62-63 0.29940613848324915 2316 64-65 0.3026433231932586 2316 66-67 0.4110282437202386 2316 68-69 0.13594275556240376 2316 70-71 -0.22547944989690194 2316 72-73 -0.1851721838411251 2316 74-75 0.06722584857633507 2316 76-77 0.05908210254456492 2316 78-79 0.09173564598550144 2316 80-81 0.07927745728097335 2316 82-83 0.012884054693451219 2316 84-85 0.14506548494314586 2316 86-87 0.19090098290569912 2316 88-89 0.0013291635685916958 2316 90-91 0.08652222650318464 2316 92-93 0.16399635572191684 2316 94-95 0.422890888051473 2316 96-97 0.40983253153521915 2316 98-99 0.3952390192214317 2316 100 -0.4281086634840001 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 7.0 11 15.0 12 41.0 13 213.0 14 725.0 15 2292.0 16 5986.0 17 13026.0 18 22663.0 19 34403.0 20 49729.0 21 72614.0 22 85490.0 23 76470.0 24 67595.0 25 69950.0 26 83014.0 27 102177.0 28 126948.0 29 157159.0 30 195224.0 31 244084.0 32 308686.0 33 404727.0 34 565977.0 35 785471.0 36 1218705.0 37 1874322.0 38 1695127.0 39 236274.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.30885142979275 24.535238499656806 15.632964935687339 21.522945134863104 2 13.978400114649672 22.457228165320497 42.33668598908993 21.227685730939903 3 16.204406718159092 30.81070568061565 31.078215917565053 21.906671683660203 4 10.665009629761702 19.582268998993186 42.96621374716889 26.78650762407623 5 10.747398541959836 40.679061733740596 36.490102458148414 12.08343726615116 6 25.287459375177225 42.747032220064355 19.39278611864719 12.572722286111235 7 24.897080436511434 34.101370342999324 25.272096041080992 15.729453179408251 8 24.640992293828766 36.33902211531005 21.472040728702837 17.54794486215834 9 25.944052520893703 16.328314891902526 23.62853037140797 34.09910221579581 10-11 22.631550801730057 28.726637103672992 29.42334042610645 19.2184716684905 12-13 23.543560309126576 26.99298720483298 29.660320215975617 19.80313227006483 14-15 21.985741919698537 28.054665249969396 27.210806354382616 22.748786475949455 16-17 19.10597236397762 30.88655330509854 28.89029805960957 21.11717627131427 18-19 18.780424111041633 29.807803009217952 32.02715669866234 19.384616181078073 20-21 19.83990443235769 29.142636212187366 31.778423649249905 19.239035706205037 22-23 20.347580475171682 28.792666373419813 31.265017405085356 19.594735746323153 24-25 19.77979827549124 29.47935408811928 31.82807482566422 18.912772810725258 26-27 19.30853595696444 29.52276246758264 31.634430257798122 19.53427131765479 28-29 18.99358426219015 28.540182120943907 32.616380295498686 19.84985332136725 30-31 19.684912952451022 28.814311563881372 31.979019141265457 19.521756342402146 32-33 19.754904066163782 29.577392197620583 31.322799075815706 19.344904660399926 34-35 19.787170306649656 29.17768093751355 31.890291007406475 19.144857748430315 36-37 19.99733560763857 28.54359770876379 31.395015350265798 20.064051333331843 38-39 19.527576040588873 29.308896092219367 31.931686803249594 19.231841063942163 40-41 19.145613269751266 28.466726342267123 33.02114345941789 19.36651692856372 42-43 19.35894389426517 28.316084311537498 31.9320102634587 20.39296153073863 44-45 19.104879040936197 29.25391230860597 31.61877140925008 20.022437241207754 46-47 19.639868253523712 28.95999006841497 31.108330764143684 20.291810913917637 48-49 20.004687341183715 29.06464142405617 30.74971438003274 20.180956854727373 50-51 19.97720413298213 28.583217624096612 30.381862252051846 21.057715990869415 52-53 19.393562530052737 29.45298310268286 30.448468631355674 20.704985735908725 54-55 20.018942554098302 29.44606973185072 30.028919813149084 20.506067900901893 56-57 20.06087920429428 29.313775770304968 29.757743998227443 20.867601027173308 58-59 19.31464693151941 28.938908322594063 30.791355275011504 20.955089470875016 60-61 19.840065638474897 30.230374761992284 29.52699444642776 20.402565153105062 62-63 19.834702477874316 29.487165608400733 30.184106737028937 20.494025176696013 64-65 19.324271788904117 28.989720428439735 30.15569490006131 21.530312882594842 66-67 19.764070906425264 29.098941549170537 29.549677326263264 21.587310218140935 68-69 19.264437030671562 29.26331482348599 29.962737660619947 21.509510485222496 70-71 19.836813790975295 28.583137017076428 30.413218014670967 21.166831177277317 72-73 20.036736885240305 29.03544963494956 29.770216761274504 21.157596718535626 74-75 19.763238488269153 29.54370807285717 29.591299952572857 21.10175348630082 76-77 19.42110889944969 29.20915027680417 30.23144582446103 21.138294999285115 78-79 19.301057286320997 28.881275697829267 29.92075868331802 21.896908332531712 80-81 19.141289977639396 29.758346868456485 29.56479774501946 21.535565408884658 82-83 20.311096072139524 28.738080247034524 30.13604562009663 20.81477806072932 84-85 19.686765759789687 28.98160505862506 29.47242006279443 21.859209118790822 86-87 18.923520292464467 29.556614679547977 30.310948269947318 21.208916758040235 88-89 19.80147270073089 28.615820675270115 30.020415794381666 21.562290829617332 90-91 19.69622115841544 28.55753182809826 30.401673883412812 21.344573130073485 92-93 20.021815713644724 28.968552964737714 29.66476359210511 21.34486772951245 94-95 18.932119602717457 28.031616268966797 30.355622427203933 22.680641701111814 96-97 19.71757520641018 28.21091605532009 29.924476927225026 22.14703181104471 98-99 18.948665103947828 28.622796509659885 30.056262232215143 22.372276154177147 100 19.384288065125784 27.92424386341372 29.7060599094803 22.985408161980196 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3937.0 1 2562.5 2 1670.5 3 2017.5 4 1525.0 5 1372.5 6 2193.5 7 4224.0 8 6752.5 9 8107.5 10 7515.5 11 6373.5 12 6569.0 13 7573.0 14 8627.5 15 9887.5 16 11207.0 17 12587.0 18 13818.5 19 15003.0 20 16442.0 21 17900.5 22 19526.5 23 21520.5 24 23179.0 25 26736.0 26 36697.5 27 47541.5 28 52006.0 29 69370.5 30 106496.0 31 141471.0 32 196189.0 33 271435.0 34 341967.5 35 401551.5 36 473320.0 37 541810.5 38 538829.0 39 500938.0 40 440277.5 41 370116.5 42 322876.0 43 305148.5 44 308673.0 45 323345.5 46 309690.0 47 270737.0 48 255247.5 49 237310.5 50 213726.5 51 187134.5 52 162927.5 53 146866.0 54 135359.5 55 115995.5 56 98743.0 57 78291.0 58 57770.5 59 52721.0 60 42506.5 61 28034.0 62 19380.5 63 15360.0 64 10447.0 65 4424.5 66 2248.0 67 1599.5 68 1232.0 69 967.0 70 793.0 71 625.5 72 493.5 73 440.5 74 359.0 75 261.0 76 174.0 77 109.0 78 77.0 79 50.0 80 29.5 81 22.5 82 19.0 83 10.5 84 3.5 85 3.0 86 2.5 87 1.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029461873699865398 2 0.0021884618642871263 3 7.059554400926214E-5 4 0.023825996103125968 5 4.706369600617475E-4 6 7.059554400926214E-5 7 8.236146801080583E-5 8 0.00408277562853566 9 0.010165758337333748 10-11 0.001100113894144335 12-13 1.4707405001929613E-4 14-15 1.1177627801466504E-4 16-17 3.706266060486262E-4 18-19 1.7648886002315535E-5 20-21 1.0589331601389319E-4 22-23 2.764992140362767E-4 24-25 4.706369600617476E-5 26-27 1.0001035401312135E-4 28-29 0.0011471775901505097 30-31 0.0027120454823558204 32-33 0.009589228061258108 34-35 0.019078445768503094 36-37 0.022037575654891332 38-39 0.02136691798680334 40-41 0.004618125170605898 42-43 0.005006400662656839 44-45 0.008812677077156224 46-47 0.011530605521512815 48-49 1.200541938459511 50-51 0.017966565950357213 52-53 0.018331309594405067 54-55 0.00934214365722569 56-57 0.007665499487005714 58-59 0.008377337889099107 60-61 0.005247602104688485 62-63 0.0073654684249663495 64-65 0.019243168704524704 66-67 0.028908875271792846 68-69 0.02907948116981523 70-71 0.025896798727397658 72-73 0.02280236071499167 74-75 0.009665706567268142 76-77 0.014530916141906457 78-79 0.008383220851099878 80-81 0.0028708854563766605 82-83 0.014101459915850113 84-85 0.023867176837131375 86-87 0.02295531772701174 88-89 0.032438652472255955 90-91 0.02063743069870763 92-93 0.0131425371097243 94-95 0.006871299616901514 96-97 0.003841574186504015 98-99 0.015572200416043071 100 0.036109620760737586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8499120.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.32696405975745 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37774181060323 26.275398117890997 2 14.996543424202406 10.595647011347808 3 5.094326226151816 5.3990123849983505 4 2.198272624434989 3.106331919878542 5 1.1273397124006008 1.9912744751556668 6 0.6103589430856641 1.293727706756328 7 0.363369445045749 0.8985717527881629 8 0.22680531313062596 0.6409874516422127 9 0.16118977300953102 0.5124910786117347 >10 0.6028388275656301 3.6709317456232142 >50 0.06853650251042416 1.7192445606659268 >100 0.10998612039169461 9.100961720253183 >500 0.031827008082960455 8.008404126487326 >1k 0.028429106846070158 19.1538291506906 >5k 0.0020387407299970635 4.661017438906525 >10k+ 3.9642180861054006E-4 2.9721693583034923 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43381 0.5104175491109667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35455 0.4171608354747315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22174 0.2608975988102298 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 13900 0.16354634362145729 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 12522 0.1473329003473301 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 10983 0.12922514330895432 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 10147 0.11938883084366382 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 8873 0.10439904366569715 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 8767 0.10315185572153353 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.176592400154369E-5 0.0 2 0.0 0.0 0.0 1.176592400154369E-5 0.0 3 0.0 0.0 0.0 1.176592400154369E-5 0.0 4 0.0 0.0 0.0 1.176592400154369E-5 0.0 5 0.0 0.0 0.0 1.176592400154369E-5 0.0 6 0.0 0.0 0.0 1.176592400154369E-5 0.0 7 0.0 0.0 0.0 1.176592400154369E-5 0.0 8 0.0 0.0 0.0 1.176592400154369E-5 0.0 9 0.0 0.0 0.0 2.353184800308738E-5 0.0 10-11 0.0 0.0 0.0 2.9414810003859227E-5 0.0 12-13 0.0 0.0 0.0 4.1180734005402915E-5 0.0 14-15 0.0 0.0 0.0 4.706369600617476E-5 0.0 16-17 0.0 0.0 0.0 5.8829620007718454E-5 0.0 18-19 0.0 0.0 0.0 1.1177627801466504E-4 0.0 20-21 0.0 0.0 0.0 2.3531848003087381E-4 0.0 22-23 0.0 0.0 0.0 6.471258200849029E-4 0.0 24-25 0.0 0.0 0.0 0.001782537486233869 0.0 26-27 0.0 0.0 0.0 0.003647436440478544 0.0 28-29 0.0 0.0 0.0 0.008342040117094475 0.0 30-31 0.0 0.0 0.0 0.021831671984864316 0.0 32-33 0.0 0.0 0.0 0.05098174869868881 0.0 34-35 0.0 0.0 0.0 0.09718653225275087 0.0 36-37 0.0 0.0 0.0 0.1691645723321944 0.0 38-39 0.0 0.0 0.0 0.27306356422782596 0.0 40-41 0.0 0.0 0.0 0.3854987339865774 0.0 42-43 0.0 0.0 0.0 0.49682202392718305 0.0 44-45 0.0 0.0 0.0 0.6326596165250049 0.0 46-47 0.0 0.0 0.0 0.79984751362494 0.0 48-49 0.0 0.0 0.0 0.9814663165127684 0.0 50-51 0.0 0.0 0.0 1.1793632752567325 0.0 52-53 0.0 0.0 0.0 1.3716714200999633 0.0 54-55 0.0 0.0 0.0 1.5763984977268235 0.0 56-57 0.0 0.0 0.0 1.8183058951985618 0.0 58-59 0.0 0.0 0.0 2.097287719199164 0.0 60-61 0.0 0.0 0.0 2.3853646024529596 0.0 62-63 0.0 0.0 0.0 2.6718766178145503 0.0 64-65 0.0 0.0 0.0 2.9673366183793144 0.0 66-67 0.0 0.0 0.0 3.289452319769576 0.0 68-69 0.0 0.0 0.0 3.638994390007436 0.0 70-71 0.0 0.0 0.0 4.031270296218903 0.0 72-73 0.0 0.0 0.0 4.420675316973993 0.0 74-75 0.0 0.0 0.0 4.799326283191672 0.0 76-77 0.0 0.0 0.0 5.208945161381413 0.0 78-79 0.0 0.0 0.0 5.651802774875517 0.0 80-81 0.0 0.0 0.0 6.141224032605729 0.0 82-83 0.0 0.0 0.0 6.628186212219618 0.0 84-85 0.0 0.0 0.0 7.086898408305801 0.0 86-87 0.0 0.0 0.0 7.561082794454014 0.0 88 0.0 0.0 0.0 7.931632921996631 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19640 0.0 55.987946 1 GTATCAA 39035 0.0 41.21453 1 ACCTGGG 2225 0.0 37.11695 3 TACCTGG 2280 0.0 36.42439 2 CAACGCA 47990 0.0 32.52234 5 TACCCCG 320 0.0 32.251682 5 ATCAACG 48435 0.0 32.241383 3 TATCACG 225 0.0 31.2797 2 TAGGCAT 5535 0.0 31.104853 5 CATGGGT 4660 0.0 30.711857 4 AACGCAG 51640 0.0 30.55046 6 TCAACGC 51805 0.0 30.261953 4 TCTCTGC 3900 0.0 29.83058 8 TATCAAC 53350 0.0 29.646824 2 GTGGTAC 1920 0.0 28.843271 1 GGTACTC 1915 0.0 27.933521 3 ATGGGTA 3665 0.0 27.903727 5 GTACTAG 4810 0.0 27.61246 1 AGGGGTC 2975 0.0 27.121967 5 TCACGCA 260 0.0 27.071362 4 >>END_MODULE