FastQCFastQC Report
Mon 6 Jun 2016
ERR1211275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4868818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209120.42950876372869146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174510.3584237488441753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105110.21588401948891908No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89650.054.1978031
GTATCAA160900.040.751011
CAACGCA199800.031.9154385
ATCAACG200200.031.8043983
TATCAAC205350.031.4942112
TCAACGC205250.031.2279264
GTGGTAT39850.030.9246391
AACGCAG209900.030.6259866
ACGCAGA237200.026.903887
GTACATG192750.026.647711
TGGTATC43150.026.3789372
CGCAGAG243350.026.203038
TACATGG197150.025.7184452
ACATGGG196900.024.8437393
TACCTGG34850.023.6188092
GCAGAGT264250.023.598339
CATGGGG132300.022.9448184
GAGTACT164000.021.49064412-13
ATGGGGG87950.021.1046285
CAGAGTA262400.020.792210-11
Produced by FastQC (version 0.11.5)