##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211275_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4868818 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.788358899428978 33.0 31.0 34.0 30.0 34.0 2 31.826653820290673 33.0 31.0 34.0 30.0 34.0 3 31.875542893572938 34.0 31.0 34.0 30.0 34.0 4 35.49251050254908 37.0 35.0 37.0 33.0 37.0 5 35.53582265757315 37.0 35.0 37.0 33.0 37.0 6 35.60223980440427 37.0 35.0 37.0 33.0 37.0 7 35.55350251334102 37.0 35.0 37.0 33.0 37.0 8 35.55593041267922 37.0 35.0 37.0 33.0 37.0 9 37.180920091899104 39.0 37.0 39.0 34.0 39.0 10-11 37.12399128494842 39.0 37.0 39.0 33.0 39.0 12-13 37.052121479997815 39.0 37.0 39.0 33.0 39.0 14-15 38.253238362986664 40.0 38.0 41.0 33.0 41.0 16-17 38.24940642677545 40.0 38.0 41.0 33.0 41.0 18-19 38.25382043444631 40.0 38.0 41.0 33.5 41.0 20-21 38.22198036566576 40.0 38.0 41.0 33.5 41.0 22-23 38.1036856378694 40.0 38.0 41.0 33.0 41.0 24-25 37.99750154965743 40.0 38.0 41.0 33.0 41.0 26-27 37.857705299314944 40.0 37.5 41.0 33.0 41.0 28-29 37.72040903972997 40.0 37.0 41.0 32.0 41.0 30-31 37.529660278942444 40.0 37.0 41.0 32.0 41.0 32-33 37.394414003563085 40.0 36.5 41.0 32.0 41.0 34-35 37.24415412529283 40.0 36.0 41.0 31.0 41.0 36-37 37.14899560837969 40.0 36.0 41.0 31.0 41.0 38-39 36.996685232432185 39.0 36.0 41.0 31.0 41.0 40-41 36.82703142734027 39.0 35.5 41.0 30.0 41.0 42-43 36.67458272213092 39.0 35.0 41.0 30.0 41.0 44-45 36.53281320024696 39.0 35.0 41.0 30.0 41.0 46-47 36.3541193160229 39.0 35.0 41.0 29.0 41.0 48-49 36.10061096964397 39.0 35.0 40.5 28.0 41.0 50-51 35.42854672324987 38.5 34.5 39.5 27.5 40.5 52-53 35.65994498048602 38.5 35.0 40.0 28.0 40.5 54-55 36.007946795300214 39.0 35.0 40.0 28.0 41.0 56-57 35.90603263461481 39.0 35.0 40.5 28.0 41.0 58-59 35.68633865550119 38.0 35.0 40.0 28.0 41.0 60-61 35.420557617885905 38.0 34.0 40.0 27.0 41.0 62-63 35.14743691384644 37.5 34.0 40.0 26.5 41.0 64-65 34.86457236643473 37.0 34.0 40.0 26.0 41.0 66-67 34.63030421346619 36.5 34.0 39.5 26.0 41.0 68-69 34.28397641891728 36.0 33.5 39.0 26.0 41.0 70-71 33.93266815477597 35.5 33.0 39.0 25.5 41.0 72-73 33.559575342516396 35.0 33.0 38.5 25.0 40.0 74-75 33.17745405558392 35.0 33.0 37.0 24.5 39.5 76-77 32.811390875567746 35.0 32.0 37.0 24.0 39.0 78-79 32.44152358950365 35.0 32.0 36.5 23.0 39.0 80-81 32.102883389767285 35.0 32.0 36.0 22.5 38.5 82-83 31.786279133867808 35.0 31.0 35.5 21.5 37.0 84-85 31.479863593176002 35.0 31.0 35.0 20.0 37.0 86-87 31.220999737513296 34.0 31.0 35.0 20.0 36.5 88-89 30.95544904327909 34.0 31.0 35.0 19.5 36.0 90-91 30.69594396422294 34.0 30.0 35.0 19.0 36.0 92-93 30.46003835017041 34.0 30.0 35.0 18.0 35.5 94-95 30.23474321693684 34.0 29.0 35.0 18.0 35.0 96-97 30.031107139350866 34.0 29.0 35.0 17.0 35.0 98-99 29.802012315925552 34.0 29.0 35.0 16.0 35.0 100 28.09006251620003 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3046778617135075 1101 2 -0.29515208435729434 1101 3 -0.3106573351771402 1101 4 -0.21202098688656434 1101 5 -0.1361560214496862 1101 6 -0.1980120055222443 1101 7 -0.30912227448907714 1101 8 -0.3124742858190359 1101 9 -0.35390856813812377 1101 10-11 -0.42768984460157355 1101 12-13 -0.3776541451709079 1101 14-15 -0.4353942137424127 1101 16-17 -0.43114385868010885 1101 18-19 -0.4487618405570828 1101 20-21 -0.507700570984909 1101 22-23 -0.4955344995353457 1101 24-25 -0.5509751256596118 1101 26-27 -0.5709197350205315 1101 28-29 -0.5916149413065668 1101 30-31 -0.6131570096240395 1101 32-33 -0.5715427926590095 1101 34-35 -0.6537997844564387 1101 36-37 -0.6250338460086979 1101 38-39 -0.5801371244899087 1101 40-41 -0.6573658689075543 1101 42-43 -0.6262490952261359 1101 44-45 -0.6560627557966399 1101 46-47 -0.6760306235402425 1101 48-49 -0.6301154614173115 1101 50-51 -0.5338744125054191 1101 52-53 -0.31766226740450776 1101 54-55 -0.33876012024744284 1101 56-57 -0.30261819445732385 1101 58-59 -0.43436577645549335 1101 60-61 -0.4322690696154865 1101 62-63 -0.41286090016131993 1101 64-65 -0.4586858458360581 1101 66-67 -0.5241151513166074 1101 68-69 -0.43347395015103984 1101 70-71 -0.4450467543344203 1101 72-73 -0.5978759714453545 1101 74-75 -0.530430670153244 1101 76-77 -0.5846363336788869 1101 78-79 -0.6066120760801361 1101 80-81 -0.6388568799585741 1101 82-83 -0.6398014945108734 1101 84-85 -0.5473918247063914 1101 86-87 -0.6666062969278741 1101 88-89 -0.8234468732363922 1101 90-91 -0.895587563703824 1101 92-93 -0.8719703053824084 1101 94-95 -0.8843535350840632 1101 96-97 -0.9927713626812213 1101 98-99 -1.0371342567216821 1101 100 -1.191578253901266 1102 1 -0.3710746078202227 1102 2 -0.29669818583139573 1102 3 -0.2646772691878958 1102 4 -0.20558612315605984 1102 5 -0.2207918992693081 1102 6 -0.24605125285746965 1102 7 -0.217864859198329 1102 8 -0.244178676332389 1102 9 -0.3165354559874416 1102 10-11 -0.29216314939893095 1102 12-13 -0.3186843471731038 1102 14-15 -0.4274194865909635 1102 16-17 -0.495065891125158 1102 18-19 -0.5049861933863582 1102 20-21 -0.4961650851177666 1102 22-23 -0.5406627593664766 1102 24-25 -0.5856847450650662 1102 26-27 -0.5554251907438399 1102 28-29 -0.6600460724979698 1102 30-31 -0.6662086886194416 1102 32-33 -0.6199981961152332 1102 34-35 -0.5660695203700143 1102 36-37 -0.6040216657420672 1102 38-39 -0.6979974972104657 1102 40-41 -0.8219221242167194 1102 42-43 -0.7333991985360981 1102 44-45 -0.7745115328809078 1102 46-47 -0.6747646106527299 1102 48-49 -0.6364395997043388 1102 50-51 -0.6102906416116411 1102 52-53 -0.4028538777844446 1102 54-55 -0.4187916910344498 1102 56-57 -0.36862506224193226 1102 58-59 -0.44149407776968275 1102 60-61 -0.4650883249884359 1102 62-63 -0.49352851844299295 1102 64-65 -0.5541587035209616 1102 66-67 -0.39537639365295973 1102 68-69 -0.4715897025296485 1102 70-71 -0.4800027768394344 1102 72-73 -0.5481778698252882 1102 74-75 -0.5875890934665975 1102 76-77 -0.6384053108112795 1102 78-79 -0.6728182496630097 1102 80-81 -0.5792653040126936 1102 82-83 -0.6798853526594151 1102 84-85 -0.7730142537512599 1102 86-87 -0.7639518181694562 1102 88-89 -0.7929045072644705 1102 90-91 -0.9349242590359061 1102 92-93 -0.9404426077246271 1102 94-95 -0.9445700859802635 1102 96-97 -0.9699708105511391 1102 98-99 -1.0271936850945629 1102 100 -1.1025796376574064 1103 1 -0.38047792776809075 1103 2 -0.34408470233894306 1103 3 -0.38472854303298476 1103 4 -0.25083608945089964 1103 5 -0.2448763893931698 1103 6 -0.28642011332062367 1103 7 -0.29522691898898046 1103 8 -0.36348680912526277 1103 9 -0.5457492941295925 1103 10-11 -0.4842939602842051 1103 12-13 -0.5779323451285379 1103 14-15 -0.5858498613881551 1103 16-17 -0.589829185343703 1103 18-19 -0.5945176538467365 1103 20-21 -0.626165681520618 1103 22-23 -0.6272796683952961 1103 24-25 -0.6388097490025828 1103 26-27 -0.6709061339927516 1103 28-29 -0.6108839450030032 1103 30-31 -0.6986417686495585 1103 32-33 -0.7331390194283713 1103 34-35 -0.782083351666131 1103 36-37 -0.8951116959024077 1103 38-39 -0.9085008759166158 1103 40-41 -0.8102993299097605 1103 42-43 -0.8195531078517817 1103 44-45 -0.8988261421047383 1103 46-47 -0.9894747399671502 1103 48-49 -0.9786690743112345 1103 50-51 -0.914175271680179 1103 52-53 -0.9373294275775734 1103 54-55 -0.8520194043629274 1103 56-57 -0.8299646386951167 1103 58-59 -0.7374119785072466 1103 60-61 -0.7262746584591042 1103 62-63 -0.8093698181644058 1103 64-65 -0.803116914791211 1103 66-67 -0.8569394636744434 1103 68-69 -0.8580261734459782 1103 70-71 -0.8570370303131725 1103 72-73 -0.9712356282913674 1103 74-75 -1.0130143769994504 1103 76-77 -0.8845754700046413 1103 78-79 -0.9868103592952941 1103 80-81 -0.9302061452599162 1103 82-83 -1.0432319507061543 1103 84-85 -1.0139547841970007 1103 86-87 -0.9746280277017405 1103 88-89 -1.0796886243425519 1103 90-91 -1.1024483598011088 1103 92-93 -1.1130754816597666 1103 94-95 -1.269061169547637 1103 96-97 -1.263318535253262 1103 98-99 -1.3347904766572256 1103 100 -1.2297357179634716 1104 1 -0.364074928976283 1104 2 -0.3653916138105622 1104 3 -0.3343946899366088 1104 4 -0.19249575136397112 1104 5 -0.13454842235509545 1104 6 -0.13713867712239391 1104 7 -0.16612872507950271 1104 8 -0.1934338862326257 1104 9 -0.26173856498120784 1104 10-11 -0.26517877941699197 1104 12-13 -0.3797270507739867 1104 14-15 -0.3731367499351208 1104 16-17 -0.4337387930430765 1104 18-19 -0.5188855737963465 1104 20-21 -0.44846155405131327 1104 22-23 -0.44761930428580143 1104 24-25 -0.5053343929993233 1104 26-27 -0.5446857320885599 1104 28-29 -0.5540745500278348 1104 30-31 -0.5636223033514867 1104 32-33 -0.5926320439329587 1104 34-35 -0.5533789815260022 1104 36-37 -0.5417090120663559 1104 38-39 -0.6626187932846221 1104 40-41 -0.549910049764371 1104 42-43 -0.5559699956759019 1104 44-45 -0.54449090526834 1104 46-47 -0.6354461977475836 1104 48-49 -0.6000639410488091 1104 50-51 -0.5459415538598762 1104 52-53 -0.48318584906064643 1104 54-55 -0.48952190851930055 1104 56-57 -0.38757616401032635 1104 58-59 -0.47902612673264855 1104 60-61 -0.5088836279858526 1104 62-63 -0.5766887702395067 1104 64-65 -0.5068445710041019 1104 66-67 -0.4738191041085642 1104 68-69 -0.5137699078918061 1104 70-71 -0.5478438591734474 1104 72-73 -0.6238125084739394 1104 74-75 -0.5466514447097737 1104 76-77 -0.4807948134509772 1104 78-79 -0.4522048542818169 1104 80-81 -0.5341469903739693 1104 82-83 -0.6702710826674512 1104 84-85 -0.636332519406988 1104 86-87 -0.705983834762943 1104 88-89 -0.7305354164407767 1104 90-91 -0.668478161843673 1104 92-93 -0.6754790329149394 1104 94-95 -0.8241923150571964 1104 96-97 -0.7985448903374746 1104 98-99 -0.8386848012578518 1104 100 -1.092203783515572 1105 1 -0.3824132440783252 1105 2 -0.34023083931552733 1105 3 -0.3507600472364949 1105 4 -0.29361051028141816 1105 5 -0.27236479845730344 1105 6 -0.30176937791090097 1105 7 -0.3036961943621108 1105 8 -0.2784860712629964 1105 9 -0.39740894970721996 1105 10-11 -0.44521299080025756 1105 12-13 -0.47122040299537815 1105 14-15 -0.5402957226306384 1105 16-17 -0.6660965528817044 1105 18-19 -0.5931093745289786 1105 20-21 -0.5464835785796112 1105 22-23 -0.5463484400935883 1105 24-25 -0.5228263945046265 1105 26-27 -0.5658446870448373 1105 28-29 -0.6767233220292397 1105 30-31 -0.6881797410962704 1105 32-33 -0.6855866783716564 1105 34-35 -0.6792338669748901 1105 36-37 -0.6859922265679259 1105 38-39 -0.7359678723015151 1105 40-41 -0.7953945906539346 1105 42-43 -0.7924891268081424 1105 44-45 -0.7360743728074368 1105 46-47 -0.7654709693232533 1105 48-49 -0.8754064286725196 1105 50-51 -0.8101101222148515 1105 52-53 -0.6067699602805874 1105 54-55 -0.5431323878461214 1105 56-57 -0.7074481374116104 1105 58-59 -0.556147910387466 1105 60-61 -0.6572378957292031 1105 62-63 -0.7016995721669943 1105 64-65 -0.5954567672355253 1105 66-67 -0.6743305262757602 1105 68-69 -0.6694866680484708 1105 70-71 -0.6236055756219017 1105 72-73 -0.5499179226153998 1105 74-75 -0.5731543444412779 1105 76-77 -0.6158241782216152 1105 78-79 -0.5881512615163871 1105 80-81 -0.5675256939145967 1105 82-83 -0.6587517044530067 1105 84-85 -0.6453478524421321 1105 86-87 -0.6716858333412716 1105 88-89 -0.7271979566233142 1105 90-91 -0.8585087873671391 1105 92-93 -0.765016127557562 1105 94-95 -0.7509105437691623 1105 96-97 -0.8603821683277708 1105 98-99 -0.8185552794949764 1105 100 -0.8064119230371958 1106 1 -0.3826231776845006 1106 2 -0.411157434543874 1106 3 -0.3920028149644139 1106 4 -0.23480690993039843 1106 5 -0.2497364534468005 1106 6 -0.2292148683249451 1106 7 -0.3348562658984875 1106 8 -0.33526036502709644 1106 9 -0.3408975890118171 1106 10-11 -0.39120238419896225 1106 12-13 -0.3870236867429995 1106 14-15 -0.47164508533403193 1106 16-17 -0.4638530035794304 1106 18-19 -0.5509130809305276 1106 20-21 -0.5069692538879735 1106 22-23 -0.4892290563529613 1106 24-25 -0.476550922702323 1106 26-27 -0.46465052193990175 1106 28-29 -0.5758543785531387 1106 30-31 -0.5331534490795562 1106 32-33 -0.513568227127422 1106 34-35 -0.48832066868955337 1106 36-37 -0.4934571260353735 1106 38-39 -0.5510274089664904 1106 40-41 -0.5323532781663758 1106 42-43 -0.4262841540961517 1106 44-45 -0.5040569433639348 1106 46-47 -0.5747206398828268 1106 48-49 -0.6327243410716861 1106 50-51 -0.5664901711942036 1106 52-53 -0.41794500274595947 1106 54-55 -0.4823494614139747 1106 56-57 -0.3846880420332184 1106 58-59 -0.4602354745822268 1106 60-61 -0.4783100330550951 1106 62-63 -0.35265943268603195 1106 64-65 -0.37723893738031933 1106 66-67 -0.46589821889745764 1106 68-69 -0.5002539139677893 1106 70-71 -0.45898831611727786 1106 72-73 -0.4798607095149876 1106 74-75 -0.48957592084759227 1106 76-77 -0.45963287854526413 1106 78-79 -0.35978584080154263 1106 80-81 -0.5171936215762614 1106 82-83 -0.500194497416306 1106 84-85 -0.5113898789000615 1106 86-87 -0.4208575167657287 1106 88-89 -0.4598897487508573 1106 90-91 -0.46639453456279867 1106 92-93 -0.4657762853793308 1106 94-95 -0.6207381613401104 1106 96-97 -0.6313205813525293 1106 98-99 -0.6062130361108125 1106 100 -0.6563003635551041 1107 1 -0.33221246758590794 1107 2 -0.3825249670452422 1107 3 -0.3463340355072404 1107 4 -0.19539834239739662 1107 5 -0.196768559674382 1107 6 -0.21926998945371423 1107 7 -0.1953780991009353 1107 8 -0.22193440102765294 1107 9 -0.3063148186137141 1107 10-11 -0.3106637528852474 1107 12-13 -0.3639894317466741 1107 14-15 -0.44998011212540945 1107 16-17 -0.41103237242204216 1107 18-19 -0.4028722195869108 1107 20-21 -0.405684707033771 1107 22-23 -0.4050177921896321 1107 24-25 -0.4450573175224548 1107 26-27 -0.45763017687146146 1107 28-29 -0.4473284810513931 1107 30-31 -0.44734686059729967 1107 32-33 -0.45972147205356606 1107 34-35 -0.4628322021933471 1107 36-37 -0.46048593219088474 1107 38-39 -0.5023512217159265 1107 40-41 -0.5014035645654857 1107 42-43 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0.2646764724090289 2316 98-99 0.34174945282864044 2316 100 0.21900592856006185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 153.0 1 377.0 2 219.0 3 191.0 4 177.0 5 173.0 6 189.0 7 524.0 8 1435.0 9 2633.0 10 3652.0 11 5201.0 12 6851.0 13 8239.0 14 9106.0 15 10305.0 16 12117.0 17 15631.0 18 19832.0 19 24753.0 20 31143.0 21 39641.0 22 45481.0 23 45874.0 24 44387.0 25 43798.0 26 47107.0 27 53061.0 28 63708.0 29 77363.0 30 96321.0 31 123279.0 32 162032.0 33 218631.0 34 313883.0 35 457070.0 36 690423.0 37 1010793.0 38 949200.0 39 233662.0 40 203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93558246729808 19.10149822882806 13.581908921311811 23.381010382562046 2 15.885834371889215 19.938443206446543 40.705380576078824 23.470341845585416 3 18.945653252380488 25.552570768520006 29.750726116722227 25.751049862377286 4 12.840036573774402 16.564854870686315 37.89288715684098 32.702221398698306 5 13.442234049918046 37.20358375211772 34.61373881207324 14.740443385891005 6 30.790377851526934 36.67459341102817 18.040655095797987 14.494373641646908 7 27.25997993676918 32.438940092639356 22.246576367059316 18.054503603532147 8 25.732618663933682 35.22957559330169 20.283466534236265 18.754339208528364 9 26.410150573441406 16.07427525475156 20.88148137951382 36.63409279229322 10-11 25.111355886200542 26.096377006784188 27.950312026600084 20.841955080415193 12-13 25.44906577901539 23.93828204672764 28.203686545711783 22.408965628545186 14-15 22.985072173674983 25.33888471987019 26.14097994710568 25.535063159349143 16-17 22.12594528617704 28.121790552393026 26.975391405571237 22.77687275585869 18-19 21.82446359550248 27.092115201120354 29.266323487231244 21.817097716145923 20-21 22.73621279702965 26.38510065711544 29.252235155208677 21.626451390646228 22-23 22.67357282126169 26.180306280894378 28.98678007648443 22.159340821359503 24-25 22.30988905741967 26.351986813493973 29.262533963062985 22.075590166023375 26-27 22.161634837995965 26.505862289249567 29.053039632657345 22.279463240097126 28-29 21.957083338941587 26.631604028367526 29.244646668126357 22.166665964564526 30-31 22.497767359014837 26.486591619243168 29.125412982072707 21.890228039669292 32-33 22.36121132470262 26.666520776910215 28.8676376143655 22.10463028402167 34-35 22.50377802542807 26.65273779316876 28.969145894504607 21.874338286898563 36-37 22.512718725086504 26.532082418982338 28.778416796114108 22.17678205981705 38-39 22.357119366061053 26.73425131383103 28.849377751991977 22.05925156811594 40-41 22.416037484886743 26.399217701707983 29.093202473524123 22.091542339881155 42-43 22.237625929330072 26.574151946975714 28.7406219286704 22.44760019502381 44-45 22.142399145070797 26.942384759242895 28.68173410879796 22.233481986888346 46-47 22.293102867167246 26.669061590086663 28.358119694655485 22.67971584809061 48-49 22.43944774813165 26.737660633234476 28.292052000268402 22.53083961836547 50-51 22.388118591729125 26.946212935203967 28.100838377817578 22.56483009524933 52-53 22.31111802574931 27.15059177702457 27.958566565358538 22.579723631867584 54-55 22.282261757601542 27.036069580559722 28.022552105739635 22.6591165560991 56-57 22.423731596541568 27.060058111804086 27.780886563612984 22.735323728041358 58-59 22.25449570904419 26.98696432664879 28.087931020841932 22.670608943465094 60-61 22.421555302848788 27.141706987186893 27.94993940106304 22.48679830890128 62-63 22.4003298721961 27.051390150729727 27.955764409765717 22.592515567308457 64-65 22.19608285562716 26.87839964604996 27.98776094028562 22.937756558037254 66-67 22.280369034354898 26.848480227983266 27.825203510988384 23.045947226673448 68-69 22.083006363819173 26.91676747620157 27.959178997146438 23.04104716283282 70-71 22.19346166358305 26.684722420021707 27.995408585915566 23.12640733047968 72-73 22.32940852731045 26.94264745458243 27.72514938304195 23.002794635065175 74-75 22.312296363515124 27.100010963016206 27.60695028513603 22.98074238833264 76-77 22.244123370685386 26.952064295122995 27.64652272461064 23.157289609580975 78-79 22.213575843866202 26.871327230152502 27.67923897804261 23.235857947938687 80-81 22.232072046595754 27.087954398932478 27.578331107960352 23.101642446511413 82-83 22.508518029782614 26.888102048056883 27.76789859966375 22.835481322496754 84-85 22.487069003606848 26.970968682625905 27.445247359187313 23.096714954579934 86-87 22.17446812227164 27.139201408921764 27.66995144501306 23.016379023793537 88-89 22.33371553151614 26.792805083242683 27.66238986968611 23.21108951555507 90-91 22.51431149394989 26.735252905332185 27.632968050466484 23.117467550251444 92-93 22.499772364167182 26.79447433799081 27.581724347085963 23.12402895075605 94-95 22.25592510191027 26.676452766089 27.685689232454084 23.381932899546644 96-97 22.358665292849718 26.61245187067458 27.593865673013067 23.435017163462636 98-99 22.240745116422385 26.675434322185232 27.46858305306607 23.61523750832631 100 22.54187667039854 26.513178513934488 27.230755816009992 23.714188999656972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1903.0 1 1393.5 2 1954.0 3 2750.5 4 1860.5 5 1348.5 6 1937.0 7 3659.0 8 5864.5 9 6848.0 10 5876.0 11 4652.0 12 4646.5 13 5045.5 14 5460.5 15 5933.5 16 6358.5 17 6836.5 18 7319.0 19 7734.5 20 8406.0 21 9303.5 22 10534.5 23 12391.0 24 14796.5 25 17911.0 26 22397.5 27 27960.5 28 33567.0 29 40801.0 30 50865.0 31 60817.0 32 72135.0 33 84919.0 34 96948.5 35 108392.5 36 121855.0 37 136524.0 38 143733.0 39 145549.5 40 145347.5 41 144057.0 42 145624.0 43 151174.5 44 157969.0 45 168312.0 46 177488.5 47 181796.5 48 186368.0 49 188970.0 50 187251.5 51 184129.0 52 180175.0 53 174748.5 54 168376.0 55 157083.5 56 142623.0 57 125781.5 58 110457.0 59 97945.5 60 82801.0 61 67433.0 62 54711.0 63 43715.5 64 33620.0 65 24822.0 66 18291.0 67 14324.5 68 11012.0 69 8039.5 70 6173.0 71 4777.5 72 3868.5 73 3114.0 74 2371.0 75 1725.5 76 1244.0 77 857.5 78 563.0 79 370.5 80 257.0 81 180.5 82 119.5 83 97.0 84 87.5 85 64.5 86 53.5 87 48.0 88 36.5 89 27.5 90 18.5 91 19.0 92 19.5 93 14.5 94 9.5 95 5.5 96 5.0 97 2.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023352690529816478 2 0.09636835880905797 3 0.01641055385516567 4 0.017437497150232354 5 0.01969677239937907 6 0.04458987787179557 7 0.04526766044653959 8 0.03818175171057944 9 0.033375657089667346 10-11 0.036251098315854074 12-13 0.03576843496717273 14-15 0.03215359456853799 16-17 0.037781243825503436 18-19 0.036600259036176745 20-21 0.04768097718994631 22-23 0.04800959904436765 24-25 0.0665048477885187 26-27 0.05840226519044253 28-29 0.05092611800235704 30-31 0.04896465630877967 32-33 0.04373751493689023 34-35 0.04494930802506892 36-37 0.04062587675283816 38-39 0.0457914015270236 40-41 0.05429449201017578 42-43 0.056389456332111815 44-45 0.05993241070009189 46-47 0.06100043172696124 48-49 0.060764234769095905 50-51 0.0553419741711438 52-53 0.06436880573477997 54-55 0.05109042892956771 56-57 0.05506469948147579 58-59 0.053288087581010425 60-61 0.04921112269959567 62-63 0.05527008814048913 64-65 0.053288087581010425 66-67 0.05126500928972905 68-69 0.05737532189537584 70-71 0.057077508339806494 72-73 0.05930597529010121 74-75 0.050453724086626364 76-77 0.05151147568054506 78-79 0.060384265749921236 80-81 0.060938815129257244 82-83 0.05180928923611439 84-85 0.0574472079260305 86-87 0.055157124378031795 88-89 0.0637731786236413 90-91 0.07004780215649876 92-93 0.0737550674516895 94-95 0.0771029025936069 96-97 0.07272812415662282 98-99 0.08148795046354167 100 0.12746420178367726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4868818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.46203045886537 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51517709621253 47.79293322349105 2 14.59077878758633 18.22739338097568 3 4.409600246503242 8.26297754725517 4 1.7115976193865203 4.2763945054176915 5 0.853269488524871 2.664847239093048 6 0.49793083252367965 1.8661062496501364 7 0.29325029571501554 1.2821906232115803 8 0.20750390882679862 1.0368892378778494 9 0.15256299572579773 0.8576455037328474 >10 0.7076515073991839 7.5874777918074034 >50 0.03801548883964088 1.6314848815116432 >100 0.020972376727591693 2.3349358292759677 >500 0.00128712845248735 0.5281557698203339 >1k 2.815593034102528E-4 0.4226602297299019 >5k 0.0 0.0 >10k+ 1.2066827289009585E-4 1.227907987149636 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20912 0.42950876372869146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17451 0.3584237488441753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10511 0.21588401948891908 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0538865901333755E-5 0.0 5 0.0 0.0 0.0 2.0538865901333755E-5 0.0 6 0.0 0.0 0.0 2.0538865901333755E-5 0.0 7 0.0 0.0 0.0 2.0538865901333755E-5 0.0 8 0.0 0.0 0.0 2.0538865901333755E-5 0.0 9 0.0 0.0 0.0 2.0538865901333755E-5 0.0 10-11 2.0538865901333755E-5 0.0 0.0 3.0808298852000635E-5 0.0 12-13 2.0538865901333755E-5 3.0808298852000635E-5 0.0 4.107773180266751E-5 1.0269432950666877E-5 14-15 2.0538865901333755E-5 4.107773180266751E-5 0.0 4.107773180266751E-5 2.0538865901333755E-5 16-17 3.0808298852000635E-5 4.107773180266751E-5 0.0 4.107773180266751E-5 2.0538865901333755E-5 18-19 4.107773180266751E-5 5.134716475333438E-5 0.0 8.215546360533502E-5 2.0538865901333755E-5 20-21 4.107773180266751E-5 6.161659770400126E-5 1.0269432950666877E-5 1.6431092721067004E-4 2.0538865901333755E-5 22-23 4.107773180266751E-5 6.161659770400126E-5 2.0538865901333755E-5 2.670052567173388E-4 2.0538865901333755E-5 24-25 9.242489655600189E-5 6.161659770400126E-5 2.0538865901333755E-5 5.545493793360113E-4 2.0538865901333755E-5 26-27 1.0269432950666877E-4 6.161659770400126E-5 2.0538865901333755E-5 0.0012836791188333596 5.134716475333438E-5 28-29 1.0269432950666877E-4 6.161659770400126E-5 2.0538865901333755E-5 0.0035018766361774047 8.215546360533502E-5 30-31 1.2323319540800251E-4 7.188603065466813E-5 2.0538865901333755E-5 0.011357992843437565 8.215546360533502E-5 32-33 1.2323319540800251E-4 8.215546360533502E-5 2.0538865901333755E-5 0.030798029419049962 8.215546360533502E-5 34-35 1.2323319540800251E-4 8.215546360533502E-5 2.0538865901333755E-5 0.061277706416629246 8.215546360533502E-5 36-37 1.2323319540800251E-4 8.215546360533502E-5 2.0538865901333755E-5 0.11451444683288634 8.215546360533502E-5 38-39 1.2323319540800251E-4 8.215546360533502E-5 2.0538865901333755E-5 0.1943284797254693 8.215546360533502E-5 40-41 1.2323319540800251E-4 8.215546360533502E-5 2.0538865901333755E-5 0.2829639555226751 8.215546360533502E-5 42-43 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 0.37115784570300225 8.215546360533502E-5 44-45 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 0.4774978239071578 8.215546360533502E-5 46-47 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 0.6073137258365378 9.242489655600189E-5 48-49 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 0.7492270197818033 1.2323319540800251E-4 50-51 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 0.8959566777809316 1.2323319540800251E-4 52-53 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 1.0385066765691384 1.3350262835866937E-4 54-55 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 1.2012874582701591 1.4377206130933626E-4 56-57 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 1.3883862571983592 1.4377206130933626E-4 58-59 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 1.5990431353153887 1.4377206130933626E-4 60-61 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 1.817905290360001 1.4377206130933626E-4 62-63 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 2.035740502109547 1.4377206130933626E-4 64-65 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 2.2586488137367224 1.4377206130933626E-4 66-67 1.2323319540800251E-4 1.0269432950666877E-4 2.0538865901333755E-5 2.5136285644688297 1.4377206130933626E-4 68-69 1.6431092721067004E-4 1.0269432950666877E-4 2.0538865901333755E-5 2.7986669454475397 1.4377206130933626E-4 70-71 1.6431092721067004E-4 1.0269432950666877E-4 2.0538865901333755E-5 3.0993251339442143 1.4377206130933626E-4 72-73 1.6431092721067004E-4 1.0269432950666877E-4 2.0538865901333755E-5 3.3971900366783068 1.7458036016133692E-4 74-75 1.6431092721067004E-4 1.0269432950666877E-4 2.0538865901333755E-5 3.6996248370754463 1.8484979311200378E-4 76-77 1.6431092721067004E-4 1.0269432950666877E-4 2.0538865901333755E-5 4.028749893711369 1.8484979311200378E-4 78-79 1.8484979311200378E-4 1.0269432950666877E-4 2.0538865901333755E-5 4.390429052800906 2.0538865901333753E-4 80-81 1.8484979311200378E-4 1.0269432950666877E-4 2.0538865901333755E-5 4.7726368083588255 2.0538865901333753E-4 82-83 1.8484979311200378E-4 1.0269432950666877E-4 2.0538865901333755E-5 5.1598765860625715 2.0538865901333753E-4 84-85 1.8484979311200378E-4 1.0269432950666877E-4 2.0538865901333755E-5 5.547732529743358 2.0538865901333753E-4 86-87 1.8484979311200378E-4 1.0269432950666877E-4 2.0538865901333755E-5 5.954658810413534 2.0538865901333753E-4 88 1.8484979311200378E-4 1.0269432950666877E-4 2.0538865901333755E-5 6.276143408934161 2.0538865901333753E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8965 0.0 54.197803 1 GTATCAA 16090 0.0 40.75101 1 CAACGCA 19980 0.0 31.915438 5 ATCAACG 20020 0.0 31.804398 3 TATCAAC 20535 0.0 31.494211 2 TCAACGC 20525 0.0 31.227926 4 GTGGTAT 3985 0.0 30.924639 1 AACGCAG 20990 0.0 30.625986 6 ACGCAGA 23720 0.0 26.90388 7 GTACATG 19275 0.0 26.64771 1 TGGTATC 4315 0.0 26.378937 2 CGCAGAG 24335 0.0 26.20303 8 TACATGG 19715 0.0 25.718445 2 ACATGGG 19690 0.0 24.843739 3 TACCTGG 3485 0.0 23.618809 2 GCAGAGT 26425 0.0 23.59833 9 CATGGGG 13230 0.0 22.944818 4 GAGTACT 16400 0.0 21.490644 12-13 ATGGGGG 8795 0.0 21.104628 5 CAGAGTA 26240 0.0 20.7922 10-11 >>END_MODULE