FastQCFastQC Report
Mon 6 Jun 2016
ERR1211275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4868818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178940.3675224664384662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165930.34080140190083097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105280.21623318020924176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90700.055.5194471
GTATCAA157300.039.7997061
ATCAACG203100.029.9182853
CAACGCA204400.029.6775195
TATCAAC209550.029.444022
TCAACGC209900.028.8822754
AACGCAG214600.028.4196786
GTGGTAT40450.027.4968221
TGGTATC42400.025.6732672
ACGCAGA244200.024.8595987
CGCAGAG250150.024.118268
GTACATG189100.023.9986971
TACCTGG34750.023.7638532
TACATGG193350.022.9807832
GCAGAGT267700.022.167949
ACATGGG194750.022.0937143
CATGGGG132200.021.3671384
GAGTACT168250.020.54775612-13
GTACCTG41450.020.2666131
AGAGTAC253850.020.17920910-11
Produced by FastQC (version 0.11.5)