##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211275_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4868818 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83100128203601 31.0 31.0 34.0 30.0 34.0 2 31.983756221735952 33.0 31.0 34.0 30.0 34.0 3 32.03382627980754 33.0 31.0 34.0 30.0 34.0 4 35.745991737625026 37.0 35.0 37.0 35.0 37.0 5 35.63395160796727 37.0 35.0 37.0 33.0 37.0 6 35.65473961031199 37.0 35.0 37.0 33.0 37.0 7 35.56345441542485 37.0 35.0 37.0 33.0 37.0 8 35.54211453375337 37.0 35.0 37.0 33.0 37.0 9 37.10951569764982 39.0 37.0 39.0 33.0 39.0 10-11 37.04116111959823 39.0 37.0 39.0 33.0 39.0 12-13 36.96436784040808 39.0 37.0 39.0 33.0 39.0 14-15 38.10263384254659 40.0 37.0 41.0 33.0 41.0 16-17 38.07504275164938 40.0 37.0 41.0 33.0 41.0 18-19 38.090556373230626 40.0 37.0 41.0 33.0 41.0 20-21 38.058263525151276 40.0 37.0 41.0 33.0 41.0 22-23 37.93917794832339 40.0 37.0 41.0 33.0 41.0 24-25 37.83356360825153 40.0 37.0 41.0 33.0 41.0 26-27 37.7155087744089 40.0 37.0 41.0 32.0 41.0 28-29 37.59173859035191 39.5 37.0 41.0 32.0 41.0 30-31 37.581469054706915 39.0 37.0 41.0 32.0 41.0 32-33 37.48838968718896 39.0 36.0 41.0 32.0 41.0 34-35 37.330029690984546 39.0 36.0 41.0 31.5 41.0 36-37 37.15333618960495 39.0 36.0 41.0 31.0 41.0 38-39 37.020668774228156 39.0 35.5 41.0 31.0 41.0 40-41 36.89720759740865 39.0 35.0 40.5 30.5 41.0 42-43 36.7685949649381 39.0 35.0 40.0 30.0 41.0 44-45 36.58417258562551 39.0 35.0 40.0 30.0 41.0 46-47 36.30476329162437 39.0 35.0 40.0 29.5 41.0 48-49 34.763142512207274 38.0 34.0 40.0 23.0 41.0 50-51 35.44273014518103 38.0 34.0 40.0 26.5 41.0 52-53 35.51401038198594 38.0 34.0 40.0 27.0 41.0 54-55 35.33104872681624 38.0 34.0 40.0 27.0 41.0 56-57 35.10413441208934 38.0 34.0 40.0 26.0 41.0 58-59 34.857547868907815 37.0 33.5 40.0 26.0 41.0 60-61 34.59673590181436 37.0 33.0 40.0 26.0 41.0 62-63 34.267878158518144 36.5 33.0 39.0 26.0 41.0 64-65 33.92418087921956 36.0 32.5 39.0 25.0 40.0 66-67 33.58368694414127 35.5 32.0 39.0 25.0 40.0 68-69 33.2125246825821 35.0 31.5 38.0 23.5 40.0 70-71 32.84459400618384 35.0 31.0 37.5 23.5 40.0 72-73 32.46250732724042 35.0 31.0 37.0 23.0 39.0 74-75 32.08710902728342 34.5 31.0 36.5 22.0 39.0 76-77 31.240045838640917 34.0 29.5 35.5 21.5 38.0 78-79 31.712381321298103 34.0 31.0 36.0 23.0 37.5 80-81 31.594719704043158 34.0 31.0 35.0 22.5 37.0 82-83 31.3213877577679 34.0 30.0 35.0 21.5 37.0 84-85 31.026765120404995 34.0 30.0 35.0 20.5 36.0 86-87 30.742778329360434 34.0 30.0 35.0 20.0 36.0 88-89 30.489176120364327 34.0 29.0 35.0 20.0 35.5 90-91 30.248732443890898 34.0 29.0 35.0 19.0 35.0 92-93 30.027913448397534 33.5 29.0 35.0 19.0 35.0 94-95 29.798548744274278 33.0 29.0 35.0 18.0 35.0 96-97 29.57302860776476 33.0 29.0 35.0 18.0 35.0 98-99 29.324224483231866 33.0 27.0 34.5 17.5 35.0 100 26.820569181267405 30.0 23.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3875229459235072 1101 2 -0.3437303338072155 1101 3 -0.4037055347590659 1101 4 -0.3559872224139795 1101 5 -0.2622231158529047 1101 6 -0.26735485449950147 1101 7 -0.29267545696157526 1101 8 -0.3350227667980903 1101 9 -0.47982780294120886 1101 10-11 -0.42351319530080644 1101 12-13 -0.5533146686107955 1101 14-15 -0.61495337201724 1101 16-17 -0.6178573085082846 1101 18-19 -0.6633320338172339 1101 20-21 -0.7008325052377273 1101 22-23 -0.8434458513199132 1101 24-25 -0.750776086575307 1101 26-27 -0.9025058729928475 1101 28-29 -0.8191961877714817 1101 30-31 -0.7776520963576985 1101 32-33 -0.7090066013263439 1101 34-35 -0.7293283083683946 1101 36-37 -0.8348399780332159 1101 38-39 -0.7228697843895375 1101 40-41 -0.7407918674580429 1101 42-43 -0.846614140312937 1101 44-45 -0.7921777355867974 1101 46-47 -0.9399203114011314 1101 48-49 0.2590953665356608 1101 50-51 -0.6765077052271451 1101 52-53 -0.9420016925344115 1101 54-55 -0.9979140520326126 1101 56-57 -1.0376359156757289 1101 58-59 -1.2130227417241102 1101 60-61 -1.123175428219092 1101 62-63 -1.207861378211426 1101 64-65 -1.2142919561046952 1101 66-67 -1.080377735160269 1101 68-69 -1.212958018928692 1101 70-71 -1.1146270267460068 1101 72-73 -1.2312742944869086 1101 74-75 -1.2641015522954433 1101 76-77 -1.0684073544172463 1101 78-79 -1.014623864632334 1101 80-81 -0.7814360711629753 1101 82-83 -0.9410672329946586 1101 84-85 -0.9997228614813345 1101 86-87 -1.0406485600145068 1101 88-89 -0.9525398819992326 1101 90-91 -1.052729571893348 1101 92-93 -1.114862961831438 1101 94-95 -1.147492881365487 1101 96-97 -1.3264287347179078 1101 98-99 -1.3116523866897616 1101 100 -1.0501874478978728 1102 1 -0.30958393006684304 1102 2 -0.3280199528914167 1102 3 -0.3602102412921937 1102 4 -0.2800428240531545 1102 5 -0.2292682607995289 1102 6 -0.30766811705276353 1102 7 -0.34332720095748925 1102 8 -0.38226058025441034 1102 9 -0.40830634021754975 1102 10-11 -0.42596521804413356 1102 12-13 -0.5214090540978376 1102 14-15 -0.6151739651718771 1102 16-17 -0.649665063562388 1102 18-19 -0.5920820823570807 1102 20-21 -0.6667579976189515 1102 22-23 -0.647141102461589 1102 24-25 -0.778833991927506 1102 26-27 -0.6909881499820614 1102 28-29 -0.6781880372871782 1102 30-31 -0.767472295392146 1102 32-33 -0.63557878955897 1102 34-35 -0.6745386297887563 1102 36-37 -0.7714282963117824 1102 38-39 -0.8255952365976995 1102 40-41 -0.8064906533925438 1102 42-43 -0.9927303206219946 1102 44-45 -0.8727972428835216 1102 46-47 -0.8914776179782109 1102 48-49 0.21429491639774056 1102 50-51 -0.5017385092950448 1102 52-53 -0.8437811283867163 1102 54-55 -0.8940203684201151 1102 56-57 -1.0094132477446038 1102 58-59 -0.9694562408804899 1102 60-61 -0.9870400549799925 1102 62-63 -0.9441674869653269 1102 64-65 -1.039038063442561 1102 66-67 -0.9221740792556545 1102 68-69 -1.0441248764018098 1102 70-71 -1.0398315998003937 1102 72-73 -1.0602242258449337 1102 74-75 -1.121856161943807 1102 76-77 -0.9718983561035479 1102 78-79 -0.8098166493257715 1102 80-81 -0.7795280066546404 1102 82-83 -0.8459651495940719 1102 84-85 -0.8417278684547007 1102 86-87 -0.9113806594921172 1102 88-89 -0.8205258345273378 1102 90-91 -0.9546889755791348 1102 92-93 -1.1336573582506695 1102 94-95 -1.001266443466946 1102 96-97 -1.0159756446436923 1102 98-99 -1.169092130605204 1102 100 -1.1056024133304803 1103 1 -0.45471651630060705 1103 2 -0.37848140909718 1103 3 -0.36692318588006856 1103 4 -0.2551473100525925 1103 5 -0.352037315135604 1103 6 -0.3373794790730784 1103 7 -0.3675561717719731 1103 8 -0.38585128241796696 1103 9 -0.5767080876779076 1103 10-11 -0.5865058929285567 1103 12-13 -0.6036451846865418 1103 14-15 -0.7806477211364324 1103 16-17 -0.771400939209876 1103 18-19 -0.8026693302141297 1103 20-21 -0.8083329830973867 1103 22-23 -0.8054822005820412 1103 24-25 -0.8504813994021205 1103 26-27 -0.8965697089119118 1103 28-29 -0.9082697045775561 1103 30-31 -0.9451425159057933 1103 32-33 -0.8271034850470116 1103 34-35 -0.9080410559303544 1103 36-37 -0.8896167562526216 1103 38-39 -0.95294470157814 1103 40-41 -0.7861127597362128 1103 42-43 -0.8992484262785396 1103 44-45 -0.8742427585430832 1103 46-47 -0.8036713945144598 1103 48-49 0.3446250306256857 1103 50-51 -0.5552341912543994 1103 52-53 -0.777168468728199 1103 54-55 -0.9589364824691415 1103 56-57 -1.072116371215536 1103 58-59 -1.0902120155930888 1103 60-61 -1.1304356441609045 1103 62-63 -1.1854894054841054 1103 64-65 -1.1699571779859284 1103 66-67 -1.053663582775478 1103 68-69 -1.1366101322313327 1103 70-71 -1.138248491483136 1103 72-73 -1.216926082379807 1103 74-75 -1.489131931271988 1103 76-77 -1.1605912143054091 1103 78-79 -0.9726863403428965 1103 80-81 -0.9540414920791029 1103 82-83 -1.1034702179682974 1103 84-85 -1.1885240511404511 1103 86-87 -1.0794813180132614 1103 88-89 -1.126028286079059 1103 90-91 -1.1725693482973156 1103 92-93 -1.3098243915790881 1103 94-95 -1.295627738013934 1103 96-97 -1.264668677146389 1103 98-99 -1.502591469732046 1103 100 -1.4161617678041942 1104 1 -0.30675572571003684 1104 2 -0.3573414758058391 1104 3 -0.3960930967630816 1104 4 -0.2215361616516418 1104 5 -0.24513880317245196 1104 6 -0.3864873798155415 1104 7 -0.23479468105025347 1104 8 -0.2648472447884984 1104 9 -0.5275919774330688 1104 10-11 -0.4862416915220962 1104 12-13 -0.5183848556868682 1104 14-15 -0.5633851513040327 1104 16-17 -0.7564758315569691 1104 18-19 -0.6475004674625495 1104 20-21 -0.694485850227359 1104 22-23 -0.7472337934320308 1104 24-25 -0.7703158669203631 1104 26-27 -0.8232097248145962 1104 28-29 -0.790265129974344 1104 30-31 -0.6480927993659691 1104 32-33 -0.6676121644014117 1104 34-35 -0.6717383266580583 1104 36-37 -0.6912661950117993 1104 38-39 -0.7147420404602087 1104 40-41 -0.8227330005482827 1104 42-43 -0.7840738065488821 1104 44-45 -0.8006981897226737 1104 46-47 -0.8673064227572169 1104 48-49 0.3556216590501933 1104 50-51 -0.49622792828183293 1104 52-53 -0.794814019590973 1104 54-55 -0.7914220254838398 1104 56-57 -0.8840744407949472 1104 58-59 -0.9469194888218553 1104 60-61 -0.9577400101704754 1104 62-63 -0.9429991897652314 1104 64-65 -0.9706911539666834 1104 66-67 -1.0879721774532385 1104 68-69 -1.0412812616316742 1104 70-71 -1.0053594953142344 1104 72-73 -1.1243744761054728 1104 74-75 -1.025045432030975 1104 76-77 -0.882287767347055 1104 78-79 -0.7766120830705177 1104 80-81 -0.7101263597460523 1104 82-83 -0.914991970567975 1104 84-85 -0.940311760637659 1104 86-87 -0.789859471443183 1104 88-89 -0.9130064921737109 1104 90-91 -1.0735074259313286 1104 92-93 -1.0723919779853937 1104 94-95 -0.892368668837257 1104 96-97 -1.0859399366595994 1104 98-99 -1.0722527632191756 1104 100 -1.1088366198782573 1105 1 -0.46241245122450536 1105 2 -0.4130478458386939 1105 3 -0.41986894377991035 1105 4 -0.28287321853503045 1105 5 -0.3052313259578696 1105 6 -0.3653809329971409 1105 7 -0.618319188438015 1105 8 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1105 72-73 -0.9580976756047193 1105 74-75 -1.0105456848150887 1105 76-77 -0.9683244039372703 1105 78-79 -0.7058195320290324 1105 80-81 -0.7332081971760971 1105 82-83 -0.7002440514436863 1105 84-85 -0.6576213521157293 1105 86-87 -0.6954266264879045 1105 88-89 -0.76609726257605 1105 90-91 -0.7754171560288547 1105 92-93 -0.7743483876147543 1105 94-95 -0.805173254683119 1105 96-97 -0.8219337329954328 1105 98-99 -1.0191106757419348 1105 100 -0.8975261338358678 1106 1 -0.3551207418215405 1106 2 -0.36154305072909665 1106 3 -0.40667749625209026 1106 4 -0.31062088845520464 1106 5 -0.41807579988659427 1106 6 -0.4328463072406947 1106 7 -0.4768699189094292 1106 8 -0.43079407365065236 1106 9 -0.572612156534511 1106 10-11 -0.6371498464213303 1106 12-13 -0.5727610195602324 1106 14-15 -0.6851414640260813 1106 16-17 -0.7105054868915559 1106 18-19 -0.743159831518966 1106 20-21 -0.7914651499648784 1106 22-23 -0.8722172370354144 1106 24-25 -0.7990095958276484 1106 26-27 -0.7983394112917352 1106 28-29 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0.9078221209150001 2115 48-49 2.054103399272144 2115 50-51 1.184972733580686 2115 52-53 1.0252988533948866 2115 54-55 -0.03613490307430567 2115 56-57 0.7302768448419812 2115 58-59 0.9363397736999275 2115 60-61 1.031393352325388 2115 62-63 1.0065586576400207 2115 64-65 1.0539919938642939 2115 66-67 1.1526312265541705 2115 68-69 0.9930202341656127 2115 70-71 1.037743250534362 2115 72-73 0.9617170214836079 2115 74-75 1.0354658797921523 2115 76-77 0.7103699430210213 2115 78-79 0.6995338790375598 2115 80-81 0.6514065747213529 2115 82-83 -0.24998101460105104 2115 84-85 0.6183653951953296 2115 86-87 0.8726806912596814 2115 88-89 1.0194037704992276 2115 90-91 1.0139356846223357 2115 92-93 1.0763864692402798 2115 94-95 1.0999328449780705 2115 96-97 1.0681116689976626 2115 98-99 1.1783930132030278 2115 100 1.0242536434753617 2116 1 0.1506346817379196 2116 2 -0.08027931833942503 2116 3 0.06343034290578231 2116 4 0.046269491900623905 2116 5 0.1649397709520528 2116 6 0.0842208894430172 2116 7 0.07247025941869367 2116 8 0.0728598297940124 2116 9 0.36855912777225086 2116 10-11 0.45489157672270863 2116 12-13 0.3344602720831702 2116 14-15 0.6384384504623029 2116 16-17 0.6591517996676401 2116 18-19 0.6851088027661021 2116 20-21 0.7512106230348721 2116 22-23 0.79431749705725 2116 24-25 0.5140802498106112 2116 26-27 0.7229378764235435 2116 28-29 0.6938630840317472 2116 30-31 0.6641427212954838 2116 32-33 0.7142771705414646 2116 34-35 0.7653899382959253 2116 36-37 0.5856325133032101 2116 38-39 0.6984844894581599 2116 40-41 0.6609784805068983 2116 42-43 0.8093110153466441 2116 44-45 0.6459507803501197 2116 46-47 0.7319308353312763 2116 48-49 1.9565696849234016 2116 50-51 0.4684612739885665 2116 52-53 0.44543077982510226 2116 54-55 0.6129338528110111 2116 56-57 0.5851718111558952 2116 58-59 0.8536007619249446 2116 60-61 0.9738841981876831 2116 62-63 0.9365014793157371 2116 64-65 0.8010747935029272 2116 66-67 0.9508698497737242 2116 68-69 1.008902701843887 2116 70-71 1.0027066995125793 2116 72-73 0.9578809558234695 2116 74-75 0.9634870503686095 2116 76-77 0.7677336827546846 2116 78-79 0.8418825419270206 2116 80-81 0.8223184215783199 2116 82-83 0.8192302890853504 2116 84-85 0.7565252695370113 2116 86-87 0.22544639785779808 2116 88-89 0.2622551460401219 2116 90-91 0.6077991067319921 2116 92-93 0.5995885120917279 2116 94-95 0.6040465335974297 2116 96-97 0.5552119223665457 2116 98-99 0.7120432669003876 2116 100 0.6150583775587535 2201 1 0.5181299668550245 2201 2 0.5603583268151837 2201 3 0.5766438152971602 2201 4 0.4175689473850497 2201 5 0.4460725617116239 2201 6 0.5319586568126553 2201 7 0.6038761743150971 2201 8 0.5154111512906212 2201 9 0.6960162218150785 2201 10-11 0.7256211535050241 2201 12-13 0.6900354848093073 2201 14-15 0.796443414943468 2201 16-17 0.7826741136930337 2201 18-19 0.7731449946642996 2201 20-21 0.7620229630776336 2201 22-23 0.825505928364251 2201 24-25 0.8126637086899322 2201 26-27 0.8488847739813039 2201 28-29 0.8891981506614925 2201 30-31 0.9210305729549404 2201 32-33 1.009955221936302 2201 34-35 1.0975363728645817 2201 36-37 1.0361573866444118 2201 38-39 1.0486475573390308 2201 40-41 1.109826519524887 2201 42-43 1.1477213059053213 2201 44-45 1.1350158070471323 2201 46-47 1.1248179269931455 2201 48-49 2.272369598826181 2201 50-51 1.4760445204628994 2201 52-53 1.281856084518715 2201 54-55 1.2685969850706016 2201 56-57 1.1893603419437113 2201 58-59 1.1696543071016379 2201 60-61 1.1943257735217045 2201 62-63 1.2187469316532287 2201 64-65 1.1934922640074603 2201 66-67 1.2667199521971497 2201 68-69 1.2015467077960054 2201 70-71 1.1781314123998783 2201 72-73 1.1771979199317997 2201 74-75 1.204545651740002 2201 76-77 1.2088937665097639 2201 78-79 1.035668635464674 2201 80-81 0.9671768316956069 2201 82-83 0.9621116299792227 2201 84-85 0.5802025406198474 2201 86-87 0.4237906845370212 2201 88-89 0.5851176005156979 2201 90-91 0.7692676766190552 2201 92-93 0.8891280294275283 2201 94-95 0.9310791164032608 2201 96-97 0.9616445372571825 2201 98-99 0.8996721550543754 2201 100 1.2658624842942032 2202 1 0.5891857381732741 2202 2 0.5550706869919821 2202 3 0.5419172677565527 2202 4 0.4226411120673603 2202 5 0.4922947271895026 2202 6 0.507892920791555 2202 7 0.5349365580282637 2202 8 0.45599496751000856 2202 9 0.5714654878033585 2202 10-11 0.6478212882472718 2202 12-13 0.7298784167230608 2202 14-15 0.893662561916237 2202 16-17 0.81726474319548 2202 18-19 0.7825686293350174 2202 20-21 0.7599066003670245 2202 22-23 0.7650782670817122 2202 24-25 0.7527517946974811 2202 26-27 0.711158185607772 2202 28-29 0.7290666207247298 2202 30-31 0.8943464657099227 2202 32-33 0.951614880229279 2202 34-35 1.019693390213206 2202 36-37 0.9987238367943405 2202 38-39 1.0719128498596362 2202 40-41 1.1051028405130694 2202 42-43 1.1625587854417319 2202 44-45 1.104205801357999 2202 46-47 1.1577132233919158 2202 48-49 2.335394352203963 2202 50-51 1.5609927671297967 2202 52-53 0.9678030316251309 2202 54-55 0.9719827532442764 2202 56-57 0.8911998746923899 2202 58-59 1.0117276591337614 2202 60-61 1.1343104352956956 2202 62-63 1.2902089873563654 2202 64-65 1.221520023621295 2202 66-67 1.2061769331156427 2202 68-69 1.2651291277083558 2202 70-71 1.2456860641356329 2202 72-73 1.1781627494939642 2202 74-75 1.1490454506746026 2202 76-77 1.1687473034811653 2202 78-79 1.0356465735452218 2202 80-81 1.0206016787015493 2202 82-83 0.9797801921497786 2202 84-85 0.9257719001314975 2202 86-87 0.9271385209065279 2202 88-89 0.9854722973913148 2202 90-91 1.001902821291889 2202 92-93 1.0463795922453407 2202 94-95 0.9210938373428235 2202 96-97 0.9066968107467659 2202 98-99 0.3117902801624872 2202 100 0.8121963492223436 2203 1 0.6424362012120461 2203 2 0.60874984773249 2203 3 0.6061888089794536 2203 4 0.43556304433866444 2203 5 0.45753550575884105 2203 6 0.5372864571227538 2203 7 0.5863027399338989 2203 8 0.5308550646874366 2203 9 0.6624804862630285 2203 10-11 0.6336581851375556 2203 12-13 0.6997586981649633 2203 14-15 0.8389484361309627 2203 16-17 0.8235978744875041 2203 18-19 0.7550917073328804 2203 20-21 0.8069501255975311 2203 22-23 0.7899912574794072 2203 24-25 0.833855640763808 2203 26-27 0.8961472356658078 2203 28-29 0.7823343100743969 2203 30-31 0.8034522745239414 2203 32-33 0.9205062343804968 2203 34-35 1.0270419655224003 2203 36-37 1.0286986216821816 2203 38-39 1.0520018638332473 2203 40-41 1.0647857514581105 2203 42-43 1.183323374214794 2203 44-45 1.1814149554164146 2203 46-47 1.154786248480626 2203 48-49 2.345980995894557 2203 50-51 1.4838080103719946 2203 52-53 1.2496953869739897 2203 54-55 1.1771741705448164 2203 56-57 1.2273225849236553 2203 58-59 1.2835390371168884 2203 60-61 1.2271461161773516 2203 62-63 1.2613968748726663 2203 64-65 1.2451783457843533 2203 66-67 1.2307641852886348 2203 68-69 1.1995624419976636 2203 70-71 1.1885120498391615 2203 72-73 1.1669299996284792 2203 74-75 1.2951254753504564 2203 76-77 1.1299566791072344 2203 78-79 0.9754176060740463 2203 80-81 1.0077280151589072 2203 82-83 0.9833550898866896 2203 84-85 0.9487735827325245 2203 86-87 1.0212030544597788 2203 88-89 1.0281710155561825 2203 90-91 1.0742100620601356 2203 92-93 1.0998813108288026 2203 94-95 1.0987455785133093 2203 96-97 1.1332763104743009 2203 98-99 1.2047124877997994 2203 100 1.405119563985327 2204 1 0.6270856255011914 2204 2 0.6454377610442066 2204 3 0.5743794521208514 2204 4 0.41860475163734634 2204 5 0.5100570283934474 2204 6 0.5273414765224942 2204 7 0.5436675670842845 2204 8 0.5822731081164605 2204 9 0.7328159410674715 2204 10-11 0.4959529537328109 2204 12-13 0.7063630156219602 2204 14-15 0.8874998286750895 2204 16-17 0.8806282058113268 2204 18-19 0.854679356425919 2204 20-21 0.8416152278816398 2204 22-23 0.8035633012592598 2204 24-25 0.8469058990283926 2204 26-27 0.8725039921695057 2204 28-29 0.8773708219675243 2204 30-31 1.0166491353738891 2204 32-33 1.0220528105436628 2204 34-35 1.072713518441617 2204 36-37 1.0943455273051867 2204 38-39 1.15070971539037 2204 40-41 1.1600133244447761 2204 42-43 1.1803482754820678 2204 44-45 1.1695422312923753 2204 46-47 1.3004946500369456 2204 48-49 2.4557079599159053 2204 50-51 1.6232354740741428 2204 52-53 1.4038514958626607 2204 54-55 1.3915032990970673 2204 56-57 1.3248337419430811 2204 58-59 1.3375869887570744 2204 60-61 1.3070473513555427 2204 62-63 1.3041961724908262 2204 64-65 1.2492146431129711 2204 66-67 1.3081829515116965 2204 68-69 1.3282170682069037 2204 70-71 1.3302638293850038 2204 72-73 1.4372150402346335 2204 74-75 1.4798184831563113 2204 76-77 1.3823993343537886 2204 78-79 1.1340500148447674 2204 80-81 1.1161398893551855 2204 82-83 1.1372437994661944 2204 84-85 1.2078590039663517 2204 86-87 1.2250705582697456 2204 88-89 1.0110668164306063 2204 90-91 0.41084367799974686 2204 92-93 0.9968646372276702 2204 94-95 1.210199117855403 2204 96-97 1.2979945244212772 2204 98-99 1.261639209276062 2204 100 1.5480443749783888 2205 1 0.5359691530830624 2205 2 0.515255674293833 2205 3 0.5483609184462352 2205 4 0.422853373947504 2205 5 0.12012489874470589 2205 6 0.37866016036115724 2205 7 0.4573304389261139 2205 8 0.4819447715528966 2205 9 0.6459684291330277 2205 10-11 0.6503433501927631 2205 12-13 0.6651831801460801 2205 14-15 0.8941980938415384 2205 16-17 0.8552254949510427 2205 18-19 0.7738816603645802 2205 20-21 0.8221415216132542 2205 22-23 0.8326840073702755 2205 24-25 0.8282980675663794 2205 26-27 0.8521954876974007 2205 28-29 0.8979917056026494 2205 30-31 0.9372959240854115 2205 32-33 1.0057021367823609 2205 34-35 0.9721501383826734 2205 36-37 1.01291070553701 2205 38-39 0.9764400036911027 2205 40-41 1.0860273666409483 2205 42-43 1.1157780380109585 2205 44-45 1.1658157275367032 2205 46-47 1.215022226370344 2205 48-49 2.4392335016411906 2205 50-51 1.5634255553398475 2205 52-53 1.3002737169778271 2205 54-55 1.294508129808655 2205 56-57 1.1953166944891862 2205 58-59 1.2056131816676512 2205 60-61 1.2307619463673163 2205 62-63 1.244989883155796 2205 64-65 1.3233836128484668 2205 66-67 1.3240604068993775 2205 68-69 1.234643725447789 2205 70-71 1.299462693547035 2205 72-73 1.3108456651590217 2205 74-75 1.2409477262393942 2205 76-77 1.272972242989372 2205 78-79 1.1361012287696184 2205 80-81 0.8972435120330395 2205 82-83 1.0903790332958785 2205 84-85 1.1568063453353226 2205 86-87 1.1228056037420124 2205 88-89 1.2234363006301727 2205 90-91 1.2908097546854123 2205 92-93 1.2396730922776946 2205 94-95 1.261913810920575 2205 96-97 1.2543376702116618 2205 98-99 1.4120693395052122 2205 100 1.5898644359138352 2206 1 0.5840792166040494 2206 2 0.5767403369087987 2206 3 0.5442046306175499 2206 4 0.38509142788418416 2206 5 0.40877442264931574 2206 6 0.4871621446119505 2206 7 0.5484204977048677 2206 8 0.4924728131348104 2206 9 0.6370992960883868 2206 10-11 0.6245123203790897 2206 12-13 0.6480841173613854 2206 14-15 0.8287715423196573 2206 16-17 0.8086799164608252 2206 18-19 0.8432320210240078 2206 20-21 0.8252053639464378 2206 22-23 0.8582261508603111 2206 24-25 0.8893142785209349 2206 26-27 0.885884649819431 2206 28-29 0.9463619056500718 2206 30-31 1.0095042142602537 2206 32-33 0.9675571148807265 2206 34-35 0.980431561926622 2206 36-37 1.092340935410597 2206 38-39 1.1468306920638938 2206 40-41 1.1076811273577718 2206 42-43 1.0845432942788236 2206 44-45 1.0502158036620486 2206 46-47 1.0489823510411966 2206 48-49 2.2531346759682265 2206 50-51 1.4675039706847244 2206 52-53 1.203398646780279 2206 54-55 1.2224441969986373 2206 56-57 1.137101849422983 2206 58-59 1.224869664801517 2206 60-61 1.2074506342658822 2206 62-63 1.2117678311409747 2206 64-65 1.1879293098945567 2206 66-67 1.235894856113788 2206 68-69 1.3633448679519447 2206 70-71 1.2579742402642893 2206 72-73 1.245340180390329 2206 74-75 1.3048670895319994 2206 76-77 1.1637839918755724 2206 78-79 1.0358708248061106 2206 80-81 1.02769087322908 2206 82-83 1.1117953234956737 2206 84-85 1.1872726910691789 2206 86-87 1.197379972588024 2206 88-89 1.2413111087568645 2206 90-91 1.1726257185445945 2206 92-93 1.282938885166839 2206 94-95 1.1819590805415743 2206 96-97 1.3171044989046763 2206 98-99 1.399670891963062 2206 100 1.4056000126569188 2207 1 0.6022427851983103 2207 2 0.5856694875065429 2207 3 0.5747880657278017 2207 4 0.39221165218192056 2207 5 0.49038172930274015 2207 6 0.5145154084835895 2207 7 0.5268031442762933 2207 8 0.5041822472213724 2207 9 0.6891745949577768 2207 10-11 0.6582777516175824 2207 12-13 0.7049968814534822 2207 14-15 0.869888951493266 2207 16-17 0.8201191455070216 2207 18-19 0.8094294053150577 2207 20-21 0.849358704594195 2207 22-23 0.8679372522301207 2207 24-25 0.8933557933668581 2207 26-27 0.8857010396191001 2207 28-29 0.9830083731887242 2207 30-31 1.0283961423795631 2207 32-33 0.9830382388526075 2207 34-35 1.0424856966877059 2207 36-37 1.085393742426362 2207 38-39 1.1687852079182832 2207 40-41 1.153903451216138 2207 42-43 1.1233598589151867 2207 44-45 1.1873508003933537 2207 46-47 1.1738380790966545 2207 48-49 2.42628495444 2207 50-51 1.6282633320493645 2207 52-53 1.3888990154576462 2207 54-55 1.3620430608135194 2207 56-57 1.3672985216979328 2207 58-59 1.3506218109852668 2207 60-61 1.2724216911916955 2207 62-63 1.2317867907866358 2207 64-65 1.3180837933154734 2207 66-67 1.4241928019093422 2207 68-69 1.4048639897649693 2207 70-71 1.3881244794611547 2207 72-73 1.4308226536453788 2207 74-75 1.471156484799753 2207 76-77 1.4047280530025255 2207 78-79 1.2731706494626422 2207 80-81 1.2118938079516504 2207 82-83 1.1402235479657215 2207 84-85 1.1314222815965813 2207 86-87 1.2427795187727817 2207 88-89 1.3322571969759096 2207 90-91 1.2895158298826281 2207 92-93 1.2893586541290212 2207 94-95 1.377568969203537 2207 96-97 1.410480247028353 2207 98-99 1.4659801147252836 2207 100 1.7401151195606879 2208 1 0.6531402440465222 2208 2 0.6337607130905667 2208 3 0.5661956076870354 2208 4 0.4440833089317664 2208 5 0.5107309107737024 2208 6 0.5009965035110753 2208 7 0.5844813685371051 2208 8 0.5506670227189545 2208 9 0.6974535871098624 2208 10-11 0.6698541445315556 2208 12-13 0.7490840935724918 2208 14-15 0.9519450010469086 2208 16-17 0.9059438587370607 2208 18-19 0.8814431232032689 2208 20-21 0.9129199224542432 2208 22-23 0.9247080653511119 2208 24-25 0.9647188880162787 2208 26-27 1.0032345988715434 2208 28-29 0.976980570617151 2208 30-31 0.914121019728519 2208 32-33 1.0039019037245254 2208 34-35 1.0405078821608598 2208 36-37 1.0665807190704015 2208 38-39 1.0116042539754844 2208 40-41 1.0810166231679545 2208 42-43 1.0849838225311075 2208 44-45 1.1910583419770333 2208 46-47 1.2281401918617476 2208 48-49 2.3568366744338434 2208 50-51 1.5820015442171993 2208 52-53 1.3958155764464166 2208 54-55 1.3038270059519803 2208 56-57 1.3159604705193715 2208 58-59 1.3006765172925085 2208 60-61 1.3196589600825845 2208 62-63 1.3042106807931617 2208 64-65 1.3493204375279433 2208 66-67 1.3583586746877643 2208 68-69 1.311354542599119 2208 70-71 1.352642838622458 2208 72-73 1.3155496416312786 2208 74-75 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2311 32-33 0.8566522541877717 2311 34-35 0.9992053054131276 2311 36-37 1.0625232120352521 2311 38-39 1.0739730433386754 2311 40-41 0.9463195368048716 2311 42-43 1.0213841186097383 2311 44-45 1.1444615352016783 2311 46-47 1.1772567605721136 2311 48-49 -5.342229670260366 2311 50-51 -0.6864291544692591 2311 52-53 0.6752853939277301 2311 54-55 1.0794014474773377 2311 56-57 1.2003017902815145 2311 58-59 1.1928731762305915 2311 60-61 1.0984552201580087 2311 62-63 0.9881339953181083 2311 64-65 1.0349524734674915 2311 66-67 1.1641264941434457 2311 68-69 1.2086351914971942 2311 70-71 1.1686103318461605 2311 72-73 0.7472994652947875 2311 74-75 1.0811090732300137 2311 76-77 1.0604539167756108 2311 78-79 0.9203817013164652 2311 80-81 0.904016508629315 2311 82-83 0.9443432027857348 2311 84-85 1.0020208784878442 2311 86-87 1.0484546720501804 2311 88-89 1.0734120405545262 2311 90-91 1.167495821993608 2311 92-93 1.099922576790835 2311 94-95 1.196449316320269 2311 96-97 1.3345663444764284 2311 98-99 1.2794723667683883 2311 100 1.5849625652171753 2312 1 0.16500450715201964 2312 2 0.29316885171953544 2312 3 0.2790700057082631 2312 4 0.09701291666745249 2312 5 0.28497570546181095 2312 6 0.26370169438690283 2312 7 0.3670508555985279 2312 8 0.46449135473808667 2312 9 0.38244377131980656 2312 10-11 0.3773068722168986 2312 12-13 0.4099466620525547 2312 14-15 0.4497137665243258 2312 16-17 0.6019026197705486 2312 18-19 0.4309892690918602 2312 20-21 0.47459223584537824 2312 22-23 0.7717878830116547 2312 24-25 0.86302144712689 2312 26-27 0.7182285556723329 2312 28-29 0.9202796064538461 2312 30-31 0.9470941873889345 2312 32-33 0.9011919320444974 2312 34-35 0.5743585542098515 2312 36-37 0.8232672167707236 2312 38-39 0.9610950454241802 2312 40-41 0.7829098264964927 2312 42-43 0.8139538176639505 2312 44-45 0.939108774779065 2312 46-47 0.9953854136723308 2312 48-49 -4.322115722572118 2312 50-51 -0.6428371173230261 2312 52-53 0.5450398784988622 2312 54-55 0.8514401855017653 2312 56-57 0.9643083019363701 2312 58-59 1.0589545691567608 2312 60-61 1.121258869404194 2312 62-63 0.9517537049905229 2312 64-65 1.0664134300681312 2312 66-67 1.0994228821895007 2312 68-69 0.9803766765915256 2312 70-71 1.0579837606767413 2312 72-73 1.1814961837027198 2312 74-75 0.9377428153480629 2312 76-77 0.9293653746013142 2312 78-79 0.6138236788866749 2312 80-81 0.7969650005512712 2312 82-83 0.7642144498381391 2312 84-85 0.8702176937326804 2312 86-87 0.9540438612881879 2312 88-89 0.9924401730560142 2312 90-91 0.775411586929291 2312 92-93 0.953650804009353 2312 94-95 1.164787567388018 2312 96-97 1.2317250153873616 2312 98-99 1.1712454881794159 2312 100 0.7606526253978032 2313 1 0.1574864432693701 2313 2 0.33982700664269316 2313 3 0.41465016503130414 2313 4 0.15118249237032444 2313 5 0.28281133966599725 2313 6 0.338324312566634 2313 7 0.2847108804480172 2313 8 0.3540673404781316 2313 9 0.5830125495074583 2313 10-11 0.3817565725700973 2313 12-13 0.20320633408899624 2313 14-15 0.30139385011598563 2313 16-17 0.2243091845277334 2313 18-19 0.5354512056120768 2313 20-21 0.5456547937314511 2313 22-23 0.6258159820513214 2313 24-25 0.4445289986205836 2313 26-27 0.6258464589633235 2313 28-29 0.5461634070116119 2313 30-31 0.6735339942332104 2313 32-33 0.6176769419898633 2313 34-35 0.5605017915096369 2313 36-37 -0.15296161798187313 2313 38-39 0.31010128008343685 2313 40-41 0.543881339585333 2313 42-43 0.7019922465143935 2313 44-45 0.654048406116857 2313 46-47 0.5941256027736159 2313 48-49 -5.121439047535141 2313 50-51 -1.2458697152434368 2313 52-53 0.22709540026745856 2313 54-55 0.3023404759553685 2313 56-57 0.41323366217469726 2313 58-59 0.5650621522801913 2313 60-61 0.8758328600704175 2313 62-63 0.9132469588123371 2313 64-65 1.0388573033485855 2313 66-67 1.1319591931758737 2313 68-69 1.1158028659319612 2313 70-71 1.1040161781618991 2313 72-73 0.852540548058478 2313 74-75 0.9311170701954943 2313 76-77 0.9835939882028804 2313 78-79 0.8968003633097084 2313 80-81 -0.08827657481558404 2313 82-83 0.3989704358096198 2313 84-85 0.7294343776071273 2313 86-87 0.8901945345773861 2313 88-89 0.8716274172697673 2313 90-91 0.8611129721662572 2313 92-93 0.8627445476207249 2313 94-95 0.884534448022599 2313 96-97 0.9670542582836319 2313 98-99 1.070653051973565 2313 100 1.1319572922419034 2314 1 0.11258637645152447 2314 2 0.23380251841113164 2314 3 0.3036362953119749 2314 4 0.19767908322339167 2314 5 0.32102345943666677 2314 6 0.4041677286554872 2314 7 0.25238177197444145 2314 8 0.363143585657248 2314 9 0.5691218334713994 2314 10-11 0.4186738024316554 2314 12-13 0.32746753177590904 2314 14-15 0.34857168814626505 2314 16-17 0.5887649969244393 2314 18-19 0.7044894318844968 2314 20-21 0.7138436133497805 2314 22-23 0.4675233940998922 2314 24-25 0.5738113360905785 2314 26-27 0.6348269337589727 2314 28-29 0.796921007262533 2314 30-31 0.6694166884118857 2314 32-33 0.6878709658188384 2314 34-35 0.5392598740035339 2314 36-37 0.5813843915410928 2314 38-39 0.7120707929870562 2314 40-41 0.5539494005077472 2314 42-43 0.6294642899239378 2314 44-45 0.846464453969034 2314 46-47 0.9250440514352434 2314 48-49 -5.6729056335348105 2314 50-51 -1.1281444397653857 2314 52-53 -0.5405871261605242 2314 54-55 -0.22047648886454851 2314 56-57 0.18204226869440276 2314 58-59 0.7393854636803283 2314 60-61 1.001540647334501 2314 62-63 0.9346470948759631 2314 64-65 1.020524420918015 2314 66-67 1.1489319193810204 2314 68-69 1.2476030896243273 2314 70-71 0.93425099506441 2314 72-73 1.0878432084838607 2314 74-75 1.1604877889419178 2314 76-77 1.0195963128295737 2314 78-79 0.8409497544547264 2314 80-81 0.8868384549378057 2314 82-83 0.896097572054348 2314 84-85 1.0274569615177747 2314 86-87 1.0647070176468532 2314 88-89 0.9448807977397813 2314 90-91 1.144400450299333 2314 92-93 1.1414660314277434 2314 94-95 1.1753742222045531 2314 96-97 1.2006893681986135 2314 98-99 1.2732803595365638 2314 100 -0.09640535224827573 2315 1 0.15374840073903684 2315 2 0.26948280781905254 2315 3 0.3624166133588602 2315 4 0.23541309776727815 2315 5 0.33922270899281415 2315 6 0.3865754953770093 2315 7 0.43386402108152566 2315 8 0.3341158541207463 2315 9 0.504317593924192 2315 10-11 0.2983462231055043 2315 12-13 0.5291956662668511 2315 14-15 0.3838271090325236 2315 16-17 0.4722795776869404 2315 18-19 0.49574199414118425 2315 20-21 0.5270187053607387 2315 22-23 0.5116837058065187 2315 24-25 0.5515725588789664 2315 26-27 0.6813274521512511 2315 28-29 0.7584924395612589 2315 30-31 0.8489970736341732 2315 32-33 0.8783675230180066 2315 34-35 0.9157813957347258 2315 36-37 0.8632526389367001 2315 38-39 0.8892491276445469 2315 40-41 0.9066720531719454 2315 42-43 0.948987589525025 2315 44-45 0.9405933338571089 2315 46-47 0.8177230866327534 2315 48-49 -4.239473349994817 2315 50-51 -0.5157741113286036 2315 52-53 0.524398602740412 2315 54-55 0.5469588456197272 2315 56-57 0.820518339354507 2315 58-59 0.9419883085661382 2315 60-61 0.9390409995031561 2315 62-63 0.6601525395872017 2315 64-65 0.8085006050070476 2315 66-67 0.15006049744523864 2315 68-69 0.9011189976033265 2315 70-71 0.9207576528282573 2315 72-73 0.9496997075329432 2315 74-75 1.0831381226998182 2315 76-77 1.0324372090308351 2315 78-79 0.9387726386070341 2315 80-81 0.8514642766544718 2315 82-83 0.7802975036540012 2315 84-85 0.8014167257276874 2315 86-87 0.7954949769574711 2315 88-89 0.8106636098757463 2315 90-91 0.8780201824992595 2315 92-93 0.9528466709954273 2315 94-95 0.5787354056480467 2315 96-97 0.7532992319589482 2315 98-99 0.728517915978486 2315 100 0.8908952314819985 2316 1 0.2396848113031247 2316 2 0.2988742844322658 2316 3 0.21195430173124663 2316 4 0.031494763558058025 2316 5 0.21274557346890788 2316 6 0.2345371072843605 2316 7 0.35954845667276913 2316 8 0.3788153552847149 2316 9 0.5479120891603841 2316 10-11 0.5261233588527361 2316 12-13 0.46000251320329966 2316 14-15 0.568088033849321 2316 16-17 0.5463759367347407 2316 18-19 0.22301020436097474 2316 20-21 0.5802695573073677 2316 22-23 0.6942400084747362 2316 24-25 0.703588599965947 2316 26-27 0.7449147479607205 2316 28-29 0.8145627361384342 2316 30-31 0.716650631215451 2316 32-33 0.6896696242630611 2316 34-35 0.6575340634802558 2316 36-37 0.518276949873524 2316 38-39 0.5962308780965131 2316 40-41 0.37360008210463036 2316 42-43 0.31370844766493633 2316 44-45 0.4990495183420762 2316 46-47 0.4555829277019612 2316 48-49 -4.616424828240714 2316 50-51 -0.8006871748081252 2316 52-53 0.32320506793229953 2316 54-55 0.650554291185216 2316 56-57 0.6822056806457937 2316 58-59 0.5134464647982853 2316 60-61 0.7195896664119985 2316 62-63 0.6296471399551251 2316 64-65 0.5283422740681445 2316 66-67 0.6360223752214793 2316 68-69 0.43166242281339606 2316 70-71 0.058182618996035274 2316 72-73 0.24118672970713106 2316 74-75 0.5017302607223648 2316 76-77 0.5112397637293569 2316 78-79 0.41647425046972586 2316 80-81 0.37401118024343916 2316 82-83 0.24867407138717468 2316 84-85 0.3668534417370388 2316 86-87 0.4260863497540335 2316 88-89 0.2279700274271157 2316 90-91 0.31818060825670713 2316 92-93 0.4189000806181191 2316 94-95 0.6013080369833048 2316 96-97 0.7453515449810446 2316 98-99 0.7439777792055011 2316 100 -0.03568823298685331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 9.0 12 30.0 13 163.0 14 588.0 15 1709.0 16 4000.0 17 8135.0 18 13520.0 19 19547.0 20 27061.0 21 37298.0 22 45040.0 23 48389.0 24 50067.0 25 55386.0 26 65217.0 27 80549.0 28 98833.0 29 119909.0 30 146943.0 31 180315.0 32 224887.0 33 288942.0 34 386603.0 35 530424.0 36 759556.0 37 978936.0 38 640112.0 39 56643.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.055747213337845 20.402644329541083 14.980589513406548 23.561018943714522 2 15.569654571819402 20.78714410709958 39.82975061309497 23.81345070798605 3 18.570243771791745 25.642312217179704 30.690151363288322 25.097292647740232 4 12.97595198614277 17.325934155147735 37.70226264644835 31.99585121226115 5 13.214412615865543 37.528235968279596 34.72519866332292 14.532152752531942 6 30.794373500919527 37.267484633847474 17.594907018500177 14.343234846732821 7 27.003720410169368 32.757026448719174 22.05208738548042 18.18716575563104 8 25.789572706662177 35.733466758519725 19.93757607351202 18.53938446130608 9 26.18531222405192 15.956189689393508 20.691194289426825 37.16730379712775 10-11 25.158345752608046 26.374975969319596 27.79125595426859 20.675422323803765 12-13 25.375384102860387 24.084874485834636 28.18458010196736 22.355161309337614 14-15 22.841336416632316 25.47815062034653 26.077434511218446 25.60307845180271 16-17 22.072767204160453 28.26989933146778 26.969322320764633 22.688011143607138 18-19 21.763272088140496 27.17342558023586 29.317436934122092 21.74586539750155 20-21 22.697966548328495 26.38230246990284 29.305067044034327 21.614663937734335 22-23 22.5282179097335 26.240083552329573 29.120102365980976 22.111596171955952 24-25 22.259140852636538 26.40600162400896 29.330624666307443 22.004232857047057 26-27 22.129881706328952 26.577586127219867 29.12986014050647 22.162672025944712 28-29 21.92205277371475 26.730106391380076 29.32020694760903 22.02763388729614 30-31 22.434258300659447 26.490191569834582 29.25288288267009 21.82266724683588 32-33 22.363206258041078 26.696482316788167 28.942433494807958 21.9978779303628 34-35 22.460386342930448 26.716721908891927 29.071735461810967 21.75115628636666 36-37 22.495193968908605 26.54738999334818 28.89792848658605 22.05948755115717 38-39 22.33432744144101 26.69700519582834 29.017558582104307 21.951108780626345 40-41 22.35886896345476 26.406842958421688 29.24849381628708 21.98579426183647 42-43 22.24497588511121 26.588284976550604 28.8711528993427 22.295586238995483 44-45 22.165787016924398 26.88009341849452 28.775905505179818 22.178214059401263 46-47 22.300634463350818 26.70997758452022 28.42371162344048 22.565676328688475 48-49 22.54466053585783 26.831909835160804 28.235850368574017 22.38757926040735 50-51 22.390838126540675 26.92777321281841 28.116331142152834 22.565057518488086 52-53 22.28242543202154 27.15491045268986 27.974853930010685 22.587810185277913 54-55 22.302545443192056 27.047721770623777 28.017303886750145 22.63242889943402 56-57 22.43982631840243 27.034780481422317 27.79161289355193 22.733780306623323 58-59 22.237953968312983 26.98972296812791 28.12411354874437 22.648209514814738 60-61 22.443293795967143 27.136006773256728 27.95723539552463 22.463464035251498 62-63 22.43800263019923 27.04707502024514 27.950801615092267 22.564120734463362 64-65 22.171039556036476 26.902675500606772 27.98940214781631 22.936882795540438 66-67 22.195052476643006 26.876486952923816 27.853344595680802 23.07511597475237 68-69 22.02997385761904 26.91632897284582 27.93067389819366 23.123023271341477 70-71 22.079084659166195 26.700419808554148 28.01198513875664 23.208510393523014 72-73 22.179814446637074 26.93611388100377 27.732887712728783 23.151183959630373 74-75 22.130245104110756 27.088854887447077 27.614158676909824 23.16674133153234 76-77 22.07900517089125 26.958156925344063 27.6651514631755 23.297686440589185 78-79 21.970850342469554 26.91205148566426 27.726394906646085 23.390703265220107 80-81 21.92991446601439 27.13328669536148 27.571688423294226 23.365110415329898 82-83 22.146532669282895 26.899886806316374 27.799818187588464 23.153762336812267 84-85 22.143090160425256 26.953423838061124 27.47548590619358 23.428000095320034 86-87 21.86675423460449 27.09511067486921 27.672358548386818 23.365776542139486 88-89 21.898046408770313 26.819359453328513 27.67511046148571 23.607483676415463 90-91 22.011420612670314 26.719056934101143 27.721994000557732 23.54752845267081 92-93 21.981862731957712 26.806252373151125 27.589529613588088 23.622355281303076 94-95 21.6636214432069 26.619754717700033 27.75544111818839 23.96118272090468 96-97 21.690176109520376 26.607251111782297 27.70021401225166 24.002358766445663 98-99 21.514330181624267 26.617112078337758 27.65126308531697 24.217294654721005 100 21.504086638125326 26.512010907015476 27.671477110840737 24.31242534401846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1881.0 1 1371.5 2 1594.0 3 2274.5 4 1633.5 5 1145.5 6 1705.0 7 3437.5 8 5318.5 9 6051.0 10 5276.0 11 4218.0 12 4130.0 13 4455.5 14 5019.0 15 5599.5 16 6129.5 17 6685.5 18 7307.5 19 7921.5 20 8664.5 21 9775.5 22 11311.5 23 13164.5 24 15470.0 25 18639.0 26 23347.0 27 28779.5 28 34326.0 29 41894.5 30 51784.0 31 61756.5 32 73175.0 33 86115.0 34 98371.0 35 109832.5 36 122583.0 37 137239.5 38 144721.5 39 146604.5 40 146176.5 41 144241.0 42 146219.5 43 152359.0 44 159659.5 45 169220.0 46 178103.0 47 183228.0 48 188730.5 49 191139.0 50 188137.5 51 184504.5 52 180090.5 53 174663.0 54 168959.5 55 157249.0 56 141651.0 57 125240.5 58 110107.0 59 96169.5 60 80187.0 61 65035.5 62 52656.5 63 41616.5 64 31601.0 65 23375.0 66 17216.0 67 13104.5 68 9814.5 69 7072.5 70 5441.5 71 4203.0 72 3287.0 73 2673.0 74 2045.0 75 1403.0 76 939.5 77 599.0 78 372.0 79 222.0 80 119.0 81 77.5 82 53.0 83 30.5 84 20.0 85 11.5 86 7.5 87 6.5 88 5.5 89 2.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027645313503195233 2 0.0016020315403040327 3 4.107773180266751E-5 4 0.01827959065218704 5 5.75088245237345E-4 6 0.0 7 0.0 8 0.003532684935029406 9 0.007702074713000158 10-11 8.626323678560177E-4 12-13 1.0269432950666877E-4 14-15 1.4377206130933626E-4 16-17 3.594301532733407E-4 18-19 1.0269432950666877E-5 20-21 6.161659770400126E-5 22-23 2.6700525671733875E-4 24-25 5.134716475333438E-5 26-27 6.161659770400126E-5 28-29 0.0010166738621160208 30-31 0.002536549938814718 32-33 0.009396531149860192 34-35 0.017725041272851027 36-37 0.020045933119701743 38-39 0.019932969357244406 40-41 0.004559628230096093 42-43 0.005114177609432104 44-45 0.008277162958237503 46-47 0.010895868360657555 48-49 1.1964094776185925 50-51 0.01680079230729101 52-53 0.017673694108097694 54-55 0.00887279006937618 56-57 0.007578841517592154 58-59 0.00798961883561883 60-61 0.005319566268445442 62-63 0.007506955486937486 64-65 0.017468305449084356 66-67 0.02611516799354587 68-69 0.025529810315357854 70-71 0.023065146407197805 72-73 0.020538865901333754 74-75 0.0081539297628295 76-77 0.01242601387030692 78-79 0.007311836260874816 80-81 0.0022592752491467126 82-83 0.012600594230468257 84-85 0.020662099096741757 86-87 0.020097280284455076 88-89 0.02870306509711392 90-91 0.017776388437604363 92-93 0.011388801142289566 94-95 0.005432530030902777 96-97 0.0035224155020787385 98-99 0.0147571751501083 100 0.030643987924789957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4868818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.25522512128886 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95294265328748 48.67675711279332 2 14.375505293519916 18.18651647147764 3 4.301707984853784 8.16316520963916 4 1.6879801674178203 4.270942619611404 5 0.8192778051366968 2.591180100039859 6 0.4795033441093243 1.8198655186827692 7 0.2988586545556801 1.3233060031355535 8 0.20850882750091776 1.0551418258677232 9 0.148694884495387 0.8465155553825775 >10 0.6694350293086978 7.232100006903199 >50 0.036890666957700616 1.6063925218738446 >100 0.01930027793682771 2.2467327732587923 >500 9.561675331975377E-4 0.40634220557344525 >1k 3.1872246292477226E-4 0.4406156202915605 >5k 0.0 0.0 >10k+ 1.1952092359677544E-4 1.1344264554692003 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17894 0.3675224664384662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16593 0.34080140190083097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10528 0.21623318020924176 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0538865901333755E-5 2 0.0 0.0 0.0 0.0 2.0538865901333755E-5 3 0.0 0.0 0.0 0.0 2.0538865901333755E-5 4 0.0 0.0 0.0 2.0538865901333755E-5 2.0538865901333755E-5 5 0.0 0.0 0.0 2.0538865901333755E-5 2.0538865901333755E-5 6 0.0 0.0 0.0 2.0538865901333755E-5 4.107773180266751E-5 7 0.0 0.0 0.0 2.0538865901333755E-5 4.107773180266751E-5 8 0.0 0.0 0.0 2.0538865901333755E-5 4.107773180266751E-5 9 0.0 0.0 0.0 2.0538865901333755E-5 6.161659770400126E-5 10-11 2.0538865901333755E-5 0.0 0.0 2.0538865901333755E-5 6.161659770400126E-5 12-13 4.107773180266751E-5 2.0538865901333755E-5 0.0 2.0538865901333755E-5 6.161659770400126E-5 14-15 4.107773180266751E-5 5.134716475333438E-5 0.0 2.0538865901333755E-5 6.161659770400126E-5 16-17 4.107773180266751E-5 6.161659770400126E-5 0.0 2.0538865901333755E-5 6.161659770400126E-5 18-19 4.107773180266751E-5 6.161659770400126E-5 0.0 6.161659770400127E-5 6.161659770400126E-5 20-21 4.107773180266751E-5 6.161659770400126E-5 0.0 1.4377206130933626E-4 1.0269432950666877E-4 22-23 6.161659770400126E-5 7.188603065466813E-5 0.0 2.4646639081600503E-4 1.0269432950666877E-4 24-25 7.188603065466813E-5 8.215546360533502E-5 0.0 5.442799463853445E-4 1.3350262835866937E-4 26-27 8.215546360533502E-5 8.215546360533502E-5 0.0 0.001242601387030692 1.4377206130933626E-4 28-29 8.215546360533502E-5 8.215546360533502E-5 0.0 0.0034402600384734034 1.4377206130933626E-4 30-31 8.215546360533502E-5 8.215546360533502E-5 0.0 0.01127583737983223 1.4377206130933626E-4 32-33 8.215546360533502E-5 8.215546360533502E-5 0.0 0.030685065656592625 1.8484979311200378E-4 34-35 8.215546360533502E-5 8.215546360533502E-5 0.0 0.060938815129257244 1.8484979311200378E-4 36-37 8.215546360533502E-5 8.215546360533502E-5 0.0 0.11319995941520099 2.0538865901333753E-4 38-39 8.215546360533502E-5 8.215546360533502E-5 0.0 0.18878298593210918 2.1565809196400442E-4 40-41 8.215546360533502E-5 8.215546360533502E-5 0.0 0.27246859504709353 2.2592752491467128E-4 42-43 8.215546360533502E-5 1.0269432950666877E-4 0.0 0.3560515098325713 2.2592752491467128E-4 44-45 8.215546360533502E-5 1.2323319540800251E-4 0.0 0.4567843776456627 2.2592752491467128E-4 46-47 1.0269432950666877E-4 1.2323319540800251E-4 0.0 0.5798532621264545 2.2592752491467128E-4 48-49 1.0269432950666877E-4 1.2323319540800251E-4 0.0 0.7159951347534453 3.080829885200063E-4 50-51 1.0269432950666877E-4 1.2323319540800251E-4 0.0 0.8614000359019376 3.080829885200063E-4 52-53 1.335026283586694E-4 1.2323319540800251E-4 0.0 1.0028614747973739 3.080829885200063E-4 54-55 1.6431092721067004E-4 1.2323319540800251E-4 0.0 1.1644099245443145 3.080829885200063E-4 56-57 1.6431092721067004E-4 1.2323319540800251E-4 0.0 1.3495575312118877 3.080829885200063E-4 58-59 1.6431092721067004E-4 1.2323319540800251E-4 0.0 1.5583351030989452 3.080829885200063E-4 60-61 1.6431092721067004E-4 1.5404149426000315E-4 0.0 1.775092024388671 3.080829885200063E-4 62-63 1.6431092721067004E-4 1.6431092721067004E-4 0.0 1.990421494498254 3.080829885200063E-4 64-65 1.6431092721067004E-4 1.6431092721067004E-4 0.0 2.2113272667000494 3.080829885200063E-4 66-67 1.6431092721067004E-4 1.6431092721067004E-4 0.0 2.4643455557385794 3.080829885200063E-4 68-69 1.6431092721067004E-4 1.6431092721067004E-4 0.0 2.746529034357004 3.080829885200063E-4 70-71 1.7458036016133692E-4 1.6431092721067004E-4 0.0 3.044075584669626 3.080829885200063E-4 72-73 1.8484979311200378E-4 1.6431092721067004E-4 0.0 3.3386748077254067 3.080829885200063E-4 74-75 1.8484979311200378E-4 1.6431092721067004E-4 0.0 3.6384498249883235 3.080829885200063E-4 76-77 1.8484979311200378E-4 1.8484979311200378E-4 0.0 3.9650794094172346 3.080829885200063E-4 78-79 1.8484979311200378E-4 1.8484979311200378E-4 0.0 4.323924205012387 3.183524214706732E-4 80-81 1.8484979311200378E-4 1.8484979311200378E-4 0.0 4.703359213673627 3.2862185442134007E-4 82-83 1.9511922606267064E-4 1.8484979311200378E-4 0.0 5.087538700358074 3.491607203226738E-4 84-85 2.0538865901333753E-4 1.8484979311200378E-4 0.0 5.473299679716925 3.491607203226738E-4 86-87 2.0538865901333753E-4 1.8484979311200378E-4 0.0 5.876888394678133 3.594301532733407E-4 88 2.0538865901333753E-4 1.8484979311200378E-4 0.0 6.195877520991748 3.6969958622400757E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9070 0.0 55.519447 1 GTATCAA 15730 0.0 39.799706 1 ATCAACG 20310 0.0 29.918285 3 CAACGCA 20440 0.0 29.677519 5 TATCAAC 20955 0.0 29.44402 2 TCAACGC 20990 0.0 28.882275 4 AACGCAG 21460 0.0 28.419678 6 GTGGTAT 4045 0.0 27.496822 1 TGGTATC 4240 0.0 25.673267 2 ACGCAGA 24420 0.0 24.859598 7 CGCAGAG 25015 0.0 24.11826 8 GTACATG 18910 0.0 23.998697 1 TACCTGG 3475 0.0 23.763853 2 TACATGG 19335 0.0 22.980783 2 GCAGAGT 26770 0.0 22.16794 9 ACATGGG 19475 0.0 22.093714 3 CATGGGG 13220 0.0 21.367138 4 GAGTACT 16825 0.0 20.547756 12-13 GTACCTG 4145 0.0 20.266613 1 AGAGTAC 25385 0.0 20.179209 10-11 >>END_MODULE