FastQCFastQC Report
Mon 6 Jun 2016
ERR1211274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6024182
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241210.40040290947385054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201980.335282034971719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119930.19908097066124497No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105150.053.8198551
GTATCAA191200.040.472891
ATCAACG233650.032.642013
CAACGCA233450.032.5492025
TATCAAC242900.031.622362
TCAACGC242600.031.4762634
AACGCAG246400.031.2008746
GTGGTAT44300.029.9454981
GTACATG239300.028.2488141
TACATGG242550.027.5567762
ACGCAGA279250.027.2967347
CGCAGAG286000.026.5361488
TGGTATC48400.026.1422212
ACATGGG248400.025.8229143
CATGGGG178500.023.9301954
GCAGAGT310200.023.9207789
GTACTAG18400.023.7766841
ATGGGGG127950.022.623755
GAGTACT192550.022.0264312-13
CAGAGTA303650.021.18072310-11
Produced by FastQC (version 0.11.5)