FastQCFastQC Report
Mon 6 Jun 2016
ERR1211274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6024182
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207340.3441795085208249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186640.30981799686662853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117350.19479823152753353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109650.051.7827871
GTATCAA191100.038.969511
GTGGTAT50100.031.7519681
ATCAACG239950.030.3860363
CAACGCA242600.030.1837335
TATCAAC246550.029.7977622
TCAACGC249000.029.3949414
AACGCAG257050.028.7971976
TGGTATC54200.028.0441722
GTACATG237600.026.5437031
TACATGG242200.025.6067332
ACGCAGA288350.025.3784717
CGCAGAG296600.024.6409328
ACATGGG246000.024.3937853
CATGGGG172300.023.2824234
ATGGGGG120950.022.6887655
GCAGAGT322650.022.2870869
TACCTGG39050.022.1051622
GAGTACT197400.020.93197812-13
TGGGGGG105800.020.528456
Produced by FastQC (version 0.11.5)