##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211274_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6024182 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0940599072206 33.0 31.0 34.0 30.0 34.0 2 32.231129637185596 34.0 31.0 34.0 30.0 34.0 3 32.29439167010558 34.0 31.0 34.0 30.0 34.0 4 35.93573949127035 37.0 35.0 37.0 35.0 37.0 5 35.83956377811958 37.0 35.0 37.0 35.0 37.0 6 35.85762564942427 37.0 35.0 37.0 35.0 37.0 7 35.77502406135804 37.0 35.0 37.0 33.0 37.0 8 35.75500275390086 37.0 35.0 37.0 33.0 37.0 9 37.37336703970763 39.0 37.0 39.0 34.0 39.0 10-11 37.32070379015774 39.0 37.0 39.0 34.0 39.0 12-13 37.24396723073772 39.0 37.0 39.0 33.0 39.0 14-15 38.47192780696201 40.0 38.0 41.0 34.0 41.0 16-17 38.452847374133114 40.0 38.0 41.0 34.0 41.0 18-19 38.46469952269038 40.0 38.0 41.0 34.0 41.0 20-21 38.43437042572751 40.0 38.0 41.0 34.0 41.0 22-23 38.325857601911764 40.0 38.0 41.0 34.0 41.0 24-25 38.23221219080035 40.0 38.0 41.0 33.5 41.0 26-27 38.127426429015586 40.0 37.5 41.0 33.0 41.0 28-29 38.01814802408028 40.0 37.0 41.0 33.0 41.0 30-31 38.00624889819066 40.0 37.0 41.0 33.0 41.0 32-33 37.92448783917883 40.0 37.0 41.0 33.0 41.0 34-35 37.78293509392645 40.0 37.0 41.0 33.0 41.0 36-37 37.62969984970573 40.0 37.0 41.0 33.0 41.0 38-39 37.51254236674788 40.0 36.0 41.0 32.0 41.0 40-41 37.394261411756816 40.0 36.0 41.0 32.0 41.0 42-43 37.27964493768614 40.0 36.0 41.0 31.5 41.0 44-45 37.1130099489026 39.0 36.0 41.0 31.0 41.0 46-47 36.8603900081372 39.0 35.5 41.0 30.5 41.0 48-49 35.284580462542465 39.0 35.0 40.5 24.0 41.0 50-51 36.02190048375033 39.0 35.0 40.0 28.0 41.0 52-53 36.12117977179308 39.0 35.0 40.0 29.0 41.0 54-55 35.953754385242675 38.0 35.0 40.0 28.5 41.0 56-57 35.737992477650906 38.0 34.5 40.0 28.0 41.0 58-59 35.50080293058875 38.0 34.0 40.0 28.0 41.0 60-61 35.24294609625008 37.5 34.0 40.0 27.5 41.0 62-63 34.92386053409409 37.0 34.0 40.0 27.0 41.0 64-65 34.590229594657 36.5 33.5 39.0 26.0 41.0 66-67 34.256623139871934 36.0 33.0 39.0 26.0 40.5 68-69 33.89707698074195 35.5 33.0 39.0 26.0 40.0 70-71 33.53495769218128 35.0 32.5 38.0 25.5 40.0 72-73 33.15553539053103 35.0 32.0 37.0 25.0 39.5 74-75 32.79331160645545 35.0 32.0 37.0 25.0 39.0 76-77 31.86680033903358 34.0 30.5 36.0 23.5 38.0 78-79 32.30327893479978 34.5 31.5 36.0 25.0 38.0 80-81 32.181591127226895 35.0 31.5 35.5 25.0 37.0 82-83 31.913570589334785 34.0 31.0 35.0 24.0 37.0 84-85 31.633395704180252 34.0 31.0 35.0 24.0 36.5 86-87 31.3715261258707 34.0 31.0 35.0 23.0 36.0 88-89 31.14673585559002 34.0 31.0 35.0 22.5 36.0 90-91 30.929252552462724 34.0 30.0 35.0 20.5 35.0 92-93 30.737203075869886 34.0 30.0 35.0 20.0 35.0 94-95 30.537253439554117 34.0 30.0 35.0 20.0 35.0 96-97 30.347047200765182 34.0 29.0 35.0 19.5 35.0 98-99 30.127891139411126 34.0 29.0 35.0 19.0 35.0 100 27.742255297067718 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29136580889066366 1101 2 -0.24852839440740837 1101 3 -0.30302999014801557 1101 4 -0.13845805240726605 1101 5 -0.21686132702638616 1101 6 -0.20636262818491247 1101 7 -0.22447264683518142 1101 8 -0.22665735844952906 1101 9 -0.4010257254387639 1101 10-11 -0.33452845938231945 1101 12-13 -0.39885868778872435 1101 14-15 -0.47144188270400633 1101 16-17 -0.5167796809192922 1101 18-19 -0.544347190129912 1101 20-21 -0.6400383997289012 1101 22-23 -0.6565048664662214 1101 24-25 -0.5532533739969381 1101 26-27 -0.6570117825627833 1101 28-29 -0.6643098615351093 1101 30-31 -0.6307536245510832 1101 32-33 -0.5727001892168104 1101 34-35 -0.5860317747854751 1101 36-37 -0.6453208354099615 1101 38-39 -0.6040032932340367 1101 40-41 -0.5660509507561287 1101 42-43 -0.5727363062219339 1101 44-45 -0.733896757811138 1101 46-47 -0.553068170547732 1101 48-49 0.6453958692699899 1101 50-51 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1105 68-69 -0.7350776825477823 1105 70-71 -0.8228582803731754 1105 72-73 -0.8394709554179158 1105 74-75 -0.8953811543628376 1105 76-77 -0.8152599797723461 1105 78-79 -0.6730834031445028 1105 80-81 -0.747600314346851 1105 82-83 -0.7196503980864151 1105 84-85 -0.785076517656826 1105 86-87 -0.64582264865086 1105 88-89 -0.7453677723082848 1105 90-91 -0.7965930541229831 1105 92-93 -0.7430568769327834 1105 94-95 -0.718207203412156 1105 96-97 -0.7144568964765234 1105 98-99 -0.8579797785766274 1105 100 -0.8602210016675187 1106 1 -0.29301787171326765 1106 2 -0.27468456742044367 1106 3 -0.3397911966018192 1106 4 -0.17457384530325726 1106 5 -0.20138011565816072 1106 6 -0.3656902602211147 1106 7 -0.3109283927064439 1106 8 -0.3836197464019122 1106 9 -0.39035213211101905 1106 10-11 -0.38435684181440877 1106 12-13 -0.428926591243588 1106 14-15 -0.5580563353514307 1106 16-17 -0.540295416079509 1106 18-19 -0.5159375969453421 1106 20-21 -0.6207550673179796 1106 22-23 -0.5724670268818812 1106 24-25 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0.4059145681890257 2311 10-11 0.3741242789379342 2311 12-13 0.48882356288335416 2311 14-15 0.4480330484427171 2311 16-17 0.4483573369733804 2311 18-19 0.5835445558205805 2311 20-21 0.6063152600826527 2311 22-23 0.6354242644724337 2311 24-25 0.610073363496042 2311 26-27 0.6580768733133695 2311 28-29 0.7569856455666581 2311 30-31 0.6620151555711331 2311 32-33 0.6409911704241082 2311 34-35 0.7312655514398045 2311 36-37 0.7321080644373268 2311 38-39 0.7786017068362128 2311 40-41 0.6884709445746111 2311 42-43 0.7564404038017969 2311 44-45 0.7977363080379405 2311 46-47 0.8545098218452338 2311 48-49 -6.206429554198969 2311 50-51 -1.1251036613047418 2311 52-53 0.39222763965690177 2311 54-55 0.7929187914544187 2311 56-57 0.8965678169394451 2311 58-59 0.9450442726922574 2311 60-61 0.8272319014127376 2311 62-63 0.68527632928771 2311 64-65 0.8023649593721629 2311 66-67 0.845991570509419 2311 68-69 0.9008459742790009 2311 70-71 0.8630969970175144 2311 72-73 0.4316290771503333 2311 74-75 0.7441642116028149 2311 76-77 0.8359047755119882 2311 78-79 0.721915489001212 2311 80-81 0.752280931682229 2311 82-83 0.8074898028756827 2311 84-85 0.8439105431390139 2311 86-87 0.8494279533277869 2311 88-89 0.8957299244226107 2311 90-91 0.8669813242773259 2311 92-93 0.9051295789479212 2311 94-95 0.889576273808764 2311 96-97 0.9940001428029142 2311 98-99 1.0056098695261362 2311 100 1.2184454621149925 2312 1 0.04338132116693316 2312 2 0.18719566524333686 2312 3 0.18861717818577972 2312 4 -0.015010759588840017 2312 5 0.16364037536116172 2312 6 0.10604312701204321 2312 7 0.26937419899429926 2312 8 0.32000763402201926 2312 9 0.21794378668320746 2312 10-11 0.21479325082079725 2312 12-13 0.2951039747838635 2312 14-15 0.3364682494266802 2312 16-17 0.48502994177415104 2312 18-19 0.374414245444342 2312 20-21 0.360963612172263 2312 22-23 0.6251773021393845 2312 24-25 0.6966259116546993 2312 26-27 0.5804807927428044 2312 28-29 0.6783541312070298 2312 30-31 0.7486677794062686 2312 32-33 0.7907121275464348 2312 34-35 0.4557169767566265 2312 36-37 0.7174519117257177 2312 38-39 0.8733906747255773 2312 40-41 0.6139711008541937 2312 42-43 0.6469644062564655 2312 44-45 0.8132042593124993 2312 46-47 0.8695404947035499 2312 48-49 -4.6682618301816134 2312 50-51 -0.6946126021267673 2312 52-53 0.5508396760696641 2312 54-55 0.8482449121461642 2312 56-57 0.9107680635946664 2312 58-59 0.924130599679259 2312 60-61 0.9825979964610809 2312 62-63 0.745953998073027 2312 64-65 0.9434974354005519 2312 66-67 0.921317822909657 2312 68-69 0.8415281957051235 2312 70-71 0.891860962285655 2312 72-73 1.0038202117728403 2312 74-75 0.674594676589507 2312 76-77 0.8286936215088652 2312 78-79 0.5746955850808959 2312 80-81 0.6795727583810987 2312 82-83 0.7118109846438223 2312 84-85 0.8717047424856581 2312 86-87 0.9254059556897225 2312 88-89 0.9638188467917814 2312 90-91 0.7323596692511565 2312 92-93 0.8656826879445525 2312 94-95 1.0137589226765868 2312 96-97 1.040062382894785 2312 98-99 1.064746082969446 2312 100 0.6812239269559761 2313 1 0.0778557814814036 2313 2 0.25822186200230846 2313 3 0.35306556485053875 2313 4 0.17761216537561353 2313 5 0.20091774951397667 2313 6 0.31869467012472796 2313 7 0.1882649186003107 2313 8 0.2743422268270521 2313 9 0.488622593802269 2313 10-11 0.2764758306671311 2313 12-13 0.03928034263908131 2313 14-15 0.14229041655715235 2313 16-17 0.06806322756010985 2313 18-19 0.44160930461899284 2313 20-21 0.4194297996721019 2313 22-23 0.4924246552150251 2313 24-25 0.30448400005477083 2313 26-27 0.42538407406041756 2313 28-29 0.41637073976279737 2313 30-31 0.6208572475936549 2313 32-33 0.5087649370763714 2313 34-35 0.4238389964463778 2313 36-37 -0.21798876307569515 2313 38-39 0.23818938105885934 2313 40-41 0.45238861463058555 2313 42-43 0.5891072861877973 2313 44-45 0.5900169000848052 2313 46-47 0.4938304759703911 2313 48-49 -5.176480530401292 2313 50-51 -1.102606158787566 2313 52-53 0.33712063521162605 2313 54-55 0.40638555203756965 2313 56-57 0.4591178216109526 2313 58-59 0.514977248370414 2313 60-61 0.8045705293754892 2313 62-63 0.836799585304739 2313 64-65 0.968182766249349 2313 66-67 1.0195571761259714 2313 68-69 1.0568069310495076 2313 70-71 1.0338026936497826 2313 72-73 0.7501458948074671 2313 74-75 0.8757772405439894 2313 76-77 0.8847659275653577 2313 78-79 0.8819353012562559 2313 80-81 -0.10144540473209673 2313 82-83 0.31291071070116416 2313 84-85 0.7127717791971229 2313 86-87 0.850185181638647 2313 88-89 0.9071628982619089 2313 90-91 0.9947990877051645 2313 92-93 1.0454172622000755 2313 94-95 0.8871642125458408 2313 96-97 0.855127889061837 2313 98-99 1.0811171822486862 2313 100 1.1730588905207817 2314 1 0.08798409472451851 2314 2 0.22245715335752436 2314 3 0.2381355373351397 2314 4 0.07218630130951453 2314 5 0.18275860821957934 2314 6 0.25360767492107783 2314 7 0.11619946138305437 2314 8 0.28005133696080975 2314 9 0.419547403408572 2314 10-11 0.34214670670234426 2314 12-13 0.1955701987058447 2314 14-15 0.28037595781416513 2314 16-17 0.45977216755462536 2314 18-19 0.5438514636105367 2314 20-21 0.567905698803365 2314 22-23 0.3067170506739032 2314 24-25 0.4741539071873859 2314 26-27 0.49274863664321344 2314 28-29 0.6207085808297705 2314 30-31 0.4893929486195958 2314 32-33 0.5011697251209952 2314 34-35 0.3345084511172516 2314 36-37 0.45175326871295596 2314 38-39 0.4288806234181095 2314 40-41 0.24026386337412475 2314 42-43 0.26121743315746215 2314 44-45 0.5003893090156168 2314 46-47 0.5722811259269349 2314 48-49 -6.510504918133808 2314 50-51 -1.5963268298229778 2314 52-53 -0.7650105738020088 2314 54-55 -0.29913365533004566 2314 56-57 -0.022783880322513994 2314 58-59 0.46341539487153227 2314 60-61 0.6710701907509886 2314 62-63 0.4432401851531793 2314 64-65 0.6429378153720293 2314 66-67 0.6771161259381984 2314 68-69 0.7343478141325477 2314 70-71 0.5376557602756193 2314 72-73 0.7430173228754668 2314 74-75 0.6817709027141277 2314 76-77 0.6635308303080656 2314 78-79 0.5420681662688267 2314 80-81 0.5959006660602526 2314 82-83 0.5347667549739263 2314 84-85 0.593123609846895 2314 86-87 0.6465953375015232 2314 88-89 0.5377218435042082 2314 90-91 0.8110569278436195 2314 92-93 0.8436737193884056 2314 94-95 0.8012547029462702 2314 96-97 0.7791536177546199 2314 98-99 0.8568539967018722 2314 100 -0.5314978676158972 2315 1 0.05577034887414101 2315 2 0.15896674647392928 2315 3 0.3198234245938494 2315 4 0.12445218120863188 2315 5 0.21462817201216922 2315 6 0.29709058512613495 2315 7 0.3090121286325882 2315 8 0.2708919210703229 2315 9 0.4779764461876326 2315 10-11 0.2704608149084251 2315 12-13 0.381554688747606 2315 14-15 0.2812959169540008 2315 16-17 0.4284914196055283 2315 18-19 0.4517582979012431 2315 20-21 0.3397882462402677 2315 22-23 0.42911998435960186 2315 24-25 0.43398429530416394 2315 26-27 0.5173894867247171 2315 28-29 0.6454284696146573 2315 30-31 0.5925788663972966 2315 32-33 0.6077459645869681 2315 34-35 0.6562531063873891 2315 36-37 0.5194398227372261 2315 38-39 0.6334122594840252 2315 40-41 0.7114377114768828 2315 42-43 0.7258644016144942 2315 44-45 0.7209032420617376 2315 46-47 0.6068688449771855 2315 48-49 -4.78187313283172 2315 50-51 -0.925115668928143 2315 52-53 0.2725230429558181 2315 54-55 0.18162973781198843 2315 56-57 0.5805217109758516 2315 58-59 0.7219040691277598 2315 60-61 0.6055874686190563 2315 62-63 0.35492720103643904 2315 64-65 0.5224640559590981 2315 66-67 -0.17484375457087964 2315 68-69 0.5194143491230392 2315 70-71 0.5948672020803016 2315 72-73 0.732115015462746 2315 74-75 0.8396218324949913 2315 76-77 0.8067785253812509 2315 78-79 0.722255225533381 2315 80-81 0.5847509502583748 2315 82-83 0.5714877972439645 2315 84-85 0.547586190157606 2315 86-87 0.5670167393976548 2315 88-89 0.5921856873843225 2315 90-91 0.7088951199450158 2315 92-93 0.7308253294945288 2315 94-95 0.35952526525115047 2315 96-97 0.4070604272988625 2315 98-99 0.4145809710740629 2315 100 0.41468736760936054 2316 1 0.20010572487058909 2316 2 0.1732548160000249 2316 3 0.1583628173812741 2316 4 -0.03714168896600256 2316 5 0.10126153573036589 2316 6 0.0775657025758818 2316 7 0.18891932463689898 2316 8 0.2771625792749788 2316 9 0.4119136465999276 2316 10-11 0.37950618707177597 2316 12-13 0.30537441985289604 2316 14-15 0.3839776064424214 2316 16-17 0.3365750786467885 2316 18-19 0.03363313169877813 2316 20-21 0.37748556158227586 2316 22-23 0.5243746528147497 2316 24-25 0.5447403206938048 2316 26-27 0.5871922838774211 2316 28-29 0.6300189708173392 2316 30-31 0.5240188535932191 2316 32-33 0.4188394515271199 2316 34-35 0.43533643102966124 2316 36-37 0.33212832662081127 2316 38-39 0.42642350127962914 2316 40-41 0.06580640772867952 2316 42-43 -0.019109737394650494 2316 44-45 0.24725416806249711 2316 46-47 0.20680579526747067 2316 48-49 -5.125997643329249 2316 50-51 -1.16405059160477 2316 52-53 0.13120523404271722 2316 54-55 0.39412742583881055 2316 56-57 0.2826408780123586 2316 58-59 0.20328559293668746 2316 60-61 0.3257043216506972 2316 62-63 0.30954871691730546 2316 64-65 0.35713902977679624 2316 66-67 0.36503774523415444 2316 68-69 0.12100529142536232 2316 70-71 -0.27105039279939547 2316 72-73 -0.16865880139766887 2316 74-75 0.08689925362566697 2316 76-77 0.10758531184461972 2316 78-79 0.16816938763141565 2316 80-81 0.07967045973028064 2316 82-83 0.04217782774469825 2316 84-85 0.19615853448862808 2316 86-87 0.27750602064601537 2316 88-89 0.19476045046445023 2316 90-91 0.15298037769033002 2316 92-93 0.18114947227356382 2316 94-95 0.4173010236423167 2316 96-97 0.5030366044562804 2316 98-99 0.5163873651980317 2316 100 -0.18925883388454068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 6.0 11 8.0 12 43.0 13 158.0 14 529.0 15 1646.0 16 4008.0 17 8142.0 18 14012.0 19 20618.0 20 28459.0 21 39085.0 22 48642.0 23 52245.0 24 53582.0 25 57821.0 26 67539.0 27 81542.0 28 100603.0 29 123481.0 30 150710.0 31 186100.0 32 233952.0 33 303320.0 34 421303.0 35 589517.0 36 900148.0 37 1325267.0 38 1069073.0 39 142602.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44906844385524 19.540661225200072 14.267178710931663 23.743091620013033 2 15.639839929004893 20.135084594702228 40.32826937637085 23.89680609992203 3 18.77616850362514 25.422750552398927 30.102093579888646 25.698987364087287 4 12.915175040940321 16.760933593744163 37.460755320913506 32.86313604440201 5 13.422132406133832 37.50930004870387 34.4054658556659 14.663101689496397 6 31.228542715703288 36.54729325727095 17.77100543841832 14.453158588607442 7 27.50971169842591 32.440508895985474 21.951824796677656 18.097954608910957 8 26.051587364168533 35.77016416394848 19.806730404817998 18.371518067065 9 26.626928657518235 16.01143498427856 20.467775191662156 36.89386116654105 10-11 25.47564623546676 26.167241024415183 27.62447461205952 20.73263812805854 12-13 25.630187898742324 23.72898166728376 28.031877557352043 22.60895287662187 14-15 23.024476052650538 25.207796122754754 25.88511719615004 25.882610628444663 16-17 22.320791997687977 27.93796611278022 26.804538193630016 22.936703695901787 18-19 22.11757913648345 27.07441061282853 28.981142830867796 21.82686741982022 20-21 22.943694558826117 26.365414501364338 28.876935575276825 21.813955364532724 22-23 22.87502036587782 26.06242238037957 28.73630962741005 22.32624762633256 24-25 22.476244891834206 26.12459326401046 28.955328013216075 22.44383383093926 26-27 22.402653507690758 26.30963532446009 28.765979153666965 22.521732014182184 28-29 22.179207097614526 26.724286257460335 28.81114276079191 22.28536388413323 30-31 22.861657646100305 26.283114973874554 28.86228480288552 21.992942577139623 32-33 22.73724034613211 26.46073043411278 28.53083108495324 22.27119813480187 34-35 22.868097423584928 26.57366464449377 28.64062098677765 21.917616945143656 36-37 22.662396141085743 26.474945183612896 28.59406385764575 22.26859481765561 38-39 22.632256843368864 26.550050494841145 28.6240456724074 22.193646989382593 40-41 22.65800362538948 26.257662285316773 28.781100364389907 22.30323372490384 42-43 22.484345637695306 26.460561963385725 28.430040107040643 22.625052291878326 44-45 22.33195739422494 26.720173595930767 28.49537825404176 22.452490755802533 46-47 22.389935070864233 26.454862546463925 28.139957068078473 23.015245314593365 48-49 22.73247034954902 26.66182466782748 28.004471062741164 22.60123391988234 50-51 22.56390976132879 26.73288384063483 27.926260640074258 22.776945757962118 52-53 22.45975680793585 26.9757124218187 27.735802803855393 22.828727966390062 54-55 22.39028663262344 26.818440383779414 27.846593487483805 22.94467949611334 56-57 22.62796455146729 26.75746494814914 27.55138478405118 23.063185716332384 58-59 22.346168480733002 26.79908653664179 27.956969075984933 22.897775906640273 60-61 22.6696842269173 26.928977824982724 27.730126458971178 22.671211489128797 62-63 22.590426899731444 26.88154504856042 27.725382250508112 22.80264580120002 64-65 22.291966105651454 26.678367788912926 27.73886535253041 23.290800752905206 66-67 22.41162522381729 26.617974739852222 27.603995390279156 23.36640464605133 68-69 22.132631604116327 26.71884194753768 27.736840252548127 23.41168619579786 70-71 22.227934912742363 26.486390093668845 27.83386924052662 23.451805753062168 72-73 22.310861057263214 26.861241892479853 27.457268992801698 23.370628057455235 74-75 22.43214282957285 26.941179038906217 27.348757430428332 23.277920701092604 76-77 22.28407576259032 26.82810291284686 27.312051387857146 23.575769936705683 78-79 22.12488818127661 26.783668942371886 27.48527254965615 23.60617032669536 80-81 22.122122944655143 27.030274387392005 27.2464257461318 23.601176921821054 82-83 22.43082407624387 26.849058328192925 27.44848325016127 23.271634345401935 84-85 22.492941209580014 27.03728218533228 27.069293823754514 23.400482781333196 86-87 21.997773639637614 27.227794487794743 27.31158393942254 23.4628479331451 88-89 22.074862983832375 26.858357014832585 27.29494400959636 23.77183599173868 90-91 22.32648907763182 26.736520478890363 27.300242221202797 23.63674822227502 92-93 22.22997158349251 26.901516584679957 27.16993483204062 23.698576999786916 94-95 21.882633240247298 26.805242123849027 27.28333591751942 24.028788718384252 96-97 21.878205692725118 26.797211099724162 27.294177252257096 24.03040595529363 98-99 21.722647214055264 26.852499803677514 27.158131992795255 24.266720989471967 100 21.69235993659919 26.916234709203994 27.105955574596834 24.285449779599983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2174.0 1 1574.5 2 2182.0 3 2988.0 4 1972.0 5 1422.0 6 1973.5 7 3904.0 8 6198.0 9 7198.0 10 6248.5 11 4933.5 12 4858.5 13 5403.5 14 6088.5 15 6683.5 16 7414.5 17 8001.0 18 8713.0 19 9528.0 20 10244.0 21 11374.5 22 13036.5 23 15295.0 24 18267.0 25 21803.0 26 26468.0 27 32206.5 28 38560.5 29 47427.0 30 58687.5 31 69923.5 32 82788.0 33 96680.5 34 109989.0 35 123080.5 36 138287.0 37 156647.0 38 167596.5 39 171365.5 40 172857.5 41 173753.5 42 178924.0 43 188235.5 44 198988.5 45 213581.5 46 225992.5 47 233639.5 48 241375.0 49 246549.0 50 246898.5 51 243306.0 52 237047.0 53 228886.5 54 218778.5 55 201225.5 56 181388.5 57 160865.0 58 140201.0 59 121337.5 60 100435.0 61 80913.0 62 65115.0 63 51187.0 64 38635.0 65 28746.5 66 21807.0 67 17201.5 68 13644.0 69 10599.0 70 8253.5 71 6344.0 72 4995.5 73 3948.0 74 3089.5 75 2378.5 76 1748.0 77 1185.5 78 769.5 79 467.0 80 313.0 81 202.0 82 115.5 83 62.0 84 34.5 85 22.5 86 17.0 87 11.5 88 6.5 89 3.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029647178654296967 2 0.0017429752288360477 3 4.979929225245851E-5 4 0.023090271841056595 5 3.3199528168305673E-4 6 6.639905633661135E-5 7 6.639905633661135E-5 8 0.004398937482300501 9 0.009329067415293895 10-11 0.0010291853732174758 12-13 1.0789846654699344E-4 14-15 1.327981126732227E-4 16-17 3.817945739355152E-4 18-19 1.6599764084152837E-5 20-21 6.639905633661135E-5 22-23 2.0749705105191046E-4 24-25 6.639905633661135E-5 26-27 8.299882042076418E-5 28-29 0.0012200826601852335 30-31 0.002821959894305982 32-33 0.009727461753313562 34-35 0.018641535066503633 36-37 0.022376481985438023 38-39 0.021405395786515083 40-41 0.004606434533352412 42-43 0.005013128753414156 44-45 0.008938972959316302 46-47 0.011487036746233761 48-49 1.2196095669088352 50-51 0.01695665901196212 52-53 0.017969244621095445 54-55 0.009387166589588429 56-57 0.0076856907709627635 58-59 0.008532278739254558 60-61 0.005494521911854589 62-63 0.0074532940737846234 64-65 0.018475537425662106 66-67 0.02807850094834452 68-69 0.028651192809247796 70-71 0.02537273940262761 72-73 0.021604592955524915 74-75 0.0092211689487469 76-77 0.013503908082458332 78-79 0.00799278640651959 80-81 0.002697461663674836 82-83 0.013703105251468168 84-85 0.02278317620549977 86-87 0.02236818210339595 88-89 0.03153955175989039 90-91 0.019504722798879583 92-93 0.01239172388882009 94-95 0.006042314126631632 96-97 0.0038262456213972286 98-99 0.015421180834177985 100 0.03613768641120073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6024182.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.9811218535046 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66963296317526 45.534379864838535 2 15.274842610407688 18.62954077036751 3 4.9912506668923875 9.131161953574523 4 1.9626874322692518 4.787475258702132 5 0.970426059545816 2.958883489348986 6 0.5595291814697572 2.047243031747937 7 0.3475999863259847 1.4837925985694984 8 0.23788828217553154 1.1605355458293578 9 0.17609361156954656 0.9664547386271602 >10 0.753886033279075 7.8006606140493515 >50 0.03761649555544321 1.569312538656668 >100 0.017056150378748143 1.9271349820420878 >500 0.001016269383444703 0.40993211967836074 >1k 3.726309494570763E-4 0.5372030945280629 >5k 0.0 0.0 >10k+ 1.0162662257865331E-4 1.0562893994399893 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20734 0.3441795085208249 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18664 0.30981799686662853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11735 0.19479823152753353 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.6599764084152837E-5 0.0 0.0 0.0 3.319952816830567E-5 7 1.6599764084152837E-5 0.0 0.0 0.0 8.299882042076418E-5 8 1.6599764084152837E-5 0.0 0.0 0.0 9.959858450491702E-5 9 1.6599764084152837E-5 0.0 0.0 0.0 1.1619834858906986E-4 10-11 2.4899646126229255E-5 0.0 0.0 0.0 1.1619834858906986E-4 12-13 3.319952816830567E-5 0.0 0.0 1.6599764084152837E-5 1.327981126732227E-4 14-15 3.319952816830567E-5 0.0 0.0 1.6599764084152837E-5 1.6599764084152837E-4 16-17 9.959858450491702E-5 0.0 0.0 1.6599764084152837E-5 1.6599764084152837E-4 18-19 9.959858450491702E-5 0.0 0.0 2.4899646126229255E-5 1.6599764084152837E-4 20-21 9.959858450491702E-5 0.0 0.0 5.809917429453493E-5 1.6599764084152837E-4 22-23 9.959858450491702E-5 0.0 0.0 1.825974049256812E-4 2.240968151360633E-4 24-25 9.959858450491702E-5 0.0 0.0 5.643919788611963E-4 2.655962253464454E-4 26-27 9.959858450491702E-5 0.0 0.0 0.00122838254222731 2.655962253464454E-4 28-29 9.959858450491702E-5 0.0 0.0 0.0035108501037983244 2.655962253464454E-4 30-31 9.959858450491702E-5 0.0 0.0 0.013055714452186205 2.7389610738852183E-4 32-33 9.959858450491702E-5 0.0 0.0 0.03731626966117557 3.153955175989039E-4 34-35 9.959858450491702E-5 0.0 0.0 0.07298086279597794 3.153955175989039E-4 36-37 9.959858450491702E-5 0.0 0.0 0.1310302377982604 3.402951637251332E-4 38-39 9.959858450491702E-5 0.0 0.0 0.21628662613446936 3.651948098513624E-4 40-41 1.0789846654699344E-4 0.0 0.0 0.3114862731570859 3.7349469189343885E-4 42-43 1.1619834858906986E-4 0.0 0.0 0.4062792259596406 3.9009445597759163E-4 44-45 1.1619834858906986E-4 0.0 0.0 0.5226767717177203 3.983943380196681E-4 46-47 1.1619834858906986E-4 0.0 0.0 0.6579814487676501 4.56493512314203E-4 48-49 1.1619834858906986E-4 0.0 0.0 0.8027064919353366 4.647933943562794E-4 50-51 1.1619834858906986E-4 0.0 0.0 0.9603129520323257 4.647933943562794E-4 52-53 1.1619834858906986E-4 0.0 0.0 1.1193718914866781 5.145926866087379E-4 54-55 1.1619834858906986E-4 0.0 0.0 1.3042268643278043 5.643919788611964E-4 56-57 1.1619834858906986E-4 0.0 0.0 1.5050010109256329 5.726918609032729E-4 58-59 1.1619834858906986E-4 0.0 0.0 1.743108026948721 5.809917429453492E-4 60-61 1.4939787675737553E-4 0.0 0.0 1.975753720588123 5.809917429453492E-4 62-63 1.825974049256812E-4 0.0 0.0 2.2134955418013598 5.975915070295021E-4 64-65 1.825974049256812E-4 0.0 0.0 2.4566986853982833 5.975915070295021E-4 66-67 1.825974049256812E-4 0.0 0.0 2.7384630809626933 6.224911531557313E-4 68-69 1.825974049256812E-4 0.0 0.0 3.0525887166091596 6.55690681324037E-4 70-71 1.825974049256812E-4 0.0 0.0 3.3799692638768217 6.805903274502662E-4 72-73 2.4899646126229255E-4 0.0 0.0 3.692675619694093 8.133884401234889E-4 74-75 2.4899646126229255E-4 0.0 0.0 4.004991881055386 8.133884401234889E-4 76-77 2.4899646126229255E-4 0.0 0.0 4.3530806340180295 8.133884401234889E-4 78-79 2.4899646126229255E-4 0.0 0.0 4.746345644935694 8.133884401234889E-4 80-81 2.4899646126229255E-4 0.0 0.0 5.135419215422111 8.631877323759475E-4 82-83 2.4899646126229255E-4 0.0 0.0 5.534104049313251 9.461865527967116E-4 84-85 2.4899646126229255E-4 0.0 0.0 5.9340338655107026 9.461865527967116E-4 86-87 2.5729634330436894E-4 0.0 0.0 6.348007082123349 9.544864348387881E-4 88 2.655962253464454E-4 0.0 0.0 6.6811062481180015 9.627863168808645E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10965 0.0 51.782787 1 GTATCAA 19110 0.0 38.96951 1 GTGGTAT 5010 0.0 31.751968 1 ATCAACG 23995 0.0 30.386036 3 CAACGCA 24260 0.0 30.183733 5 TATCAAC 24655 0.0 29.797762 2 TCAACGC 24900 0.0 29.394941 4 AACGCAG 25705 0.0 28.797197 6 TGGTATC 5420 0.0 28.044172 2 GTACATG 23760 0.0 26.543703 1 TACATGG 24220 0.0 25.606733 2 ACGCAGA 28835 0.0 25.378471 7 CGCAGAG 29660 0.0 24.640932 8 ACATGGG 24600 0.0 24.393785 3 CATGGGG 17230 0.0 23.282423 4 ATGGGGG 12095 0.0 22.688765 5 GCAGAGT 32265 0.0 22.287086 9 TACCTGG 3905 0.0 22.105162 2 GAGTACT 19740 0.0 20.931978 12-13 TGGGGGG 10580 0.0 20.52845 6 >>END_MODULE