FastQCFastQC Report
Mon 6 Jun 2016
ERR1211273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5707419
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233020.40827561459917344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187920.3292556582931795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115390.20217544918289687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102750.053.2406121
GTATCAA185550.040.864781
ATCAACG232050.031.9340063
CAACGCA232550.031.642525
TATCAAC239150.031.3310762
TCAACGC242050.030.5753294
AACGCAG247700.030.1445476
GTACATG242700.026.97831
ACGCAGA275050.026.7885427
GTGGTAT49100.026.2490861
TACATGG247700.026.0340252
CGCAGAG284300.025.8168748
ACATGGG253550.024.8524063
TACCTGG35400.023.7837122
CATGGGG173950.023.5822624
GCAGAGT308850.023.4468759
TGGTATC52600.023.2497082
ATGGGGG119000.022.4678215
GAGTACT178400.021.69014512-13
GTACTAG18050.020.8476891
Produced by FastQC (version 0.11.5)