##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211273_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5707419 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.445098563816675 31.0 31.0 34.0 29.0 34.0 2 31.464336506571534 31.0 31.0 34.0 28.0 34.0 3 31.510971596793578 33.0 31.0 34.0 28.0 34.0 4 35.122858511001205 37.0 35.0 37.0 32.0 37.0 5 35.16524737363772 37.0 35.0 37.0 32.0 37.0 6 35.228608938646346 37.0 35.0 37.0 32.0 37.0 7 35.164686875100635 37.0 35.0 37.0 32.0 37.0 8 35.16145827036704 37.0 35.0 37.0 32.0 37.0 9 36.72921630600452 39.0 37.0 39.0 32.0 39.0 10-11 36.66068699354297 39.0 37.0 39.0 32.0 39.0 12-13 36.57676517178781 39.0 37.0 39.0 32.0 39.0 14-15 37.713298865914695 40.0 37.0 41.0 32.0 41.0 16-17 37.70945246879543 40.0 37.0 41.0 32.0 41.0 18-19 37.710952095859795 40.0 37.0 41.0 32.0 41.0 20-21 37.67386974742874 40.0 37.0 41.0 32.0 41.0 22-23 37.552200215193594 40.0 37.0 41.0 32.0 41.0 24-25 37.43970593362779 40.0 37.0 41.0 31.0 41.0 26-27 37.29252644671786 40.0 37.0 41.0 31.0 41.0 28-29 37.1481654842583 40.0 36.0 41.0 31.0 41.0 30-31 36.94728922127498 39.0 36.0 41.0 30.0 41.0 32-33 36.805589268984804 39.0 35.5 41.0 30.0 41.0 34-35 36.64667987403763 39.0 35.0 41.0 30.0 41.0 36-37 36.54657508060999 39.0 35.0 41.0 30.0 41.0 38-39 36.38610946559207 39.0 35.0 41.0 29.0 41.0 40-41 36.206099814995184 39.0 35.0 40.0 28.5 41.0 42-43 36.04603332960135 39.0 35.0 40.0 28.0 41.0 44-45 35.89519991435708 39.0 35.0 40.0 27.0 41.0 46-47 35.70817290617703 38.5 35.0 40.0 26.5 41.0 48-49 35.448433083325405 38.0 34.0 40.0 26.0 41.0 50-51 34.76814064290706 37.5 33.5 39.5 24.5 40.5 52-53 35.00843218274319 38.0 34.0 40.0 25.5 40.5 54-55 35.358259311257854 38.0 34.0 40.0 26.0 41.0 56-57 35.25544698926082 38.0 34.0 40.0 26.0 41.0 58-59 35.03195446137738 38.0 34.0 40.0 26.0 41.0 60-61 34.75864615862266 37.5 34.0 40.0 25.0 41.0 62-63 34.47694343099744 37.0 33.0 40.0 25.0 41.0 64-65 34.19445812546792 36.5 33.0 40.0 24.0 41.0 66-67 33.966809077798565 36.0 33.0 39.0 24.0 41.0 68-69 33.62145165792103 35.5 32.5 39.0 23.0 41.0 70-71 33.27547872339494 35.0 32.0 38.5 23.0 40.5 72-73 32.907802896545704 35.0 32.0 37.5 22.0 40.0 74-75 32.532315307497136 35.0 31.5 37.0 21.5 39.5 76-77 32.17433344213909 35.0 31.0 36.5 20.5 39.0 78-79 31.815017874103866 35.0 31.0 36.0 20.0 39.0 80-81 31.48558674244873 35.0 31.0 36.0 20.0 37.5 82-83 31.175329426488574 34.0 30.0 35.0 19.5 37.0 84-85 30.87184277166264 34.0 30.0 35.0 18.5 37.0 86-87 30.617428998992366 34.0 30.0 35.0 18.0 36.0 88-89 30.358978462944457 34.0 29.0 35.0 18.0 36.0 90-91 30.09919737800922 34.0 29.0 35.0 17.0 36.0 92-93 29.855893005227056 34.0 29.0 35.0 15.5 35.0 94-95 29.62784999664472 34.0 29.0 35.0 15.0 35.0 96-97 29.422022984469862 34.0 28.0 35.0 12.5 35.0 98-99 29.18311043923707 34.0 27.0 35.0 10.5 35.0 100 27.49155055901801 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31106729914470677 1101 2 -0.4028261481716484 1101 3 -0.371149966290357 1101 4 -0.2330858989888327 1101 5 -0.26043771495005785 1101 6 -0.24469812898048815 1101 7 -0.3571091620939413 1101 8 -0.36446415941743737 1101 9 -0.34714447832679696 1101 10-11 -0.36433769114902503 1101 12-13 -0.32786823821799516 1101 14-15 -0.45369970330541776 1101 16-17 -0.43934501578048213 1101 18-19 -0.5456613168559201 1101 20-21 -0.5643201203899935 1101 22-23 -0.5350955696268187 1101 24-25 -0.5273762412215675 1101 26-27 -0.6463883122493073 1101 28-29 -0.6206435152072132 1101 30-31 -0.6432107116608705 1101 32-33 -0.6630543218385796 1101 34-35 -0.6743523408868342 1101 36-37 -0.7589366314955939 1101 38-39 -0.7666970835844893 1101 40-41 -0.7413323194426482 1101 42-43 -0.7712532175016875 1101 44-45 -0.8610163943298943 1101 46-47 -0.8415387902297411 1101 48-49 -0.7062702812598545 1101 50-51 -0.7187223422993299 1101 52-53 -0.5373040127154525 1101 54-55 -0.577245550870181 1101 56-57 -0.4678226290067542 1101 58-59 -0.6274111436525089 1101 60-61 -0.5457850689335046 1101 62-63 -0.498759659053043 1101 64-65 -0.5158622386887757 1101 66-67 -0.48020503622894495 1101 68-69 -0.5932814406717668 1101 70-71 -0.5322381298316259 1101 72-73 -0.5673507466450118 1101 74-75 -0.603738524589744 1101 76-77 -0.6457390930271742 1101 78-79 -0.7055346421338449 1101 80-81 -0.8102086185849124 1101 82-83 -0.8176851911352792 1101 84-85 -0.8197724356071454 1101 86-87 -0.7560295981733418 1101 88-89 -0.8579823459595346 1101 90-91 -0.9142971885168514 1101 92-93 -0.9507160419831102 1101 94-95 -0.9774459155529662 1101 96-97 -1.1136285908219534 1101 98-99 -1.0233468305945514 1101 100 -0.9512729799189081 1102 1 -0.35935186750968384 1102 2 -0.41695434668714526 1102 3 -0.37835983267564544 1102 4 -0.21896984128979824 1102 5 -0.2513497338557116 1102 6 -0.1722523305729453 1102 7 -0.17383483802616695 1102 8 -0.3327610851229821 1102 9 -0.40819635247834185 1102 10-11 -0.37144845908660074 1102 12-13 -0.33776559418789276 1102 14-15 -0.5150808681046257 1102 16-17 -0.5115387375864273 1102 18-19 -0.5333890973252196 1102 20-21 -0.5433674104166926 1102 22-23 -0.5514214299138587 1102 24-25 -0.6176881983405167 1102 26-27 -0.6169898858383149 1102 28-29 -0.6437904650977302 1102 30-31 -0.5408866926021076 1102 32-33 -0.5363805034319356 1102 34-35 -0.5352238620499463 1102 36-37 -0.6484878929998885 1102 38-39 -0.7425758138406806 1102 40-41 -0.6635441171457188 1102 42-43 -0.701277011955348 1102 44-45 -0.7023827852247422 1102 46-47 -0.7275034264286191 1102 48-49 -0.8121181529631301 1102 50-51 -0.7106440208870666 1102 52-53 -0.5762258076849065 1102 54-55 -0.5654297525747651 1102 56-57 -0.6420672401661776 1102 58-59 -0.5584396679981367 1102 60-61 -0.6300739051434405 1102 62-63 -0.6056513896490969 1102 64-65 -0.5901572690350108 1102 66-67 -0.5120556949068984 1102 68-69 -0.4931424785878491 1102 70-71 -0.5705823467238318 1102 72-73 -0.7173610702054987 1102 74-75 -0.5947870310180008 1102 76-77 -0.6911683849507853 1102 78-79 -0.6652870042766139 1102 80-81 -0.7645510097148467 1102 82-83 -0.7858109661272863 1102 84-85 -0.8593191539715228 1102 86-87 -0.900421178942441 1102 88-89 -0.8438948230456731 1102 90-91 -0.8797138601777519 1102 92-93 -0.8353944476077864 1102 94-95 -0.8842417375654996 1102 96-97 -0.9870262833708559 1102 98-99 -0.9067298807001656 1102 100 -1.0139553966528183 1103 1 -0.4136215624725139 1103 2 -0.4293558821414223 1103 3 -0.4596522242125758 1103 4 -0.33905486787234196 1103 5 -0.2962884889767281 1103 6 -0.3495101450502247 1103 7 -0.3560984478145528 1103 8 -0.3498709245559013 1103 9 -0.5356441037294317 1103 10-11 -0.5073344414749599 1103 12-13 -0.6687137681074731 1103 14-15 -0.6965196191182699 1103 16-17 -0.6613801537781754 1103 18-19 -0.60494460341841 1103 20-21 -0.7346059679533994 1103 22-23 -0.7692007910957201 1103 24-25 -0.6820832973419542 1103 26-27 -0.6345718582938602 1103 28-29 -0.6523305395859609 1103 30-31 -0.7601001928018007 1103 32-33 -0.8284163220924867 1103 34-35 -0.845019658992257 1103 36-37 -0.9011558093518133 1103 38-39 -1.0654651020794645 1103 40-41 -0.9223069874109413 1103 42-43 -0.9048548293117094 1103 44-45 -0.9897051559079415 1103 46-47 -0.9804984267748154 1103 48-49 -0.950874623546305 1103 50-51 -0.9980122768211501 1103 52-53 -0.794826154973272 1103 54-55 -0.8476468903443575 1103 56-57 -0.9396345395120846 1103 58-59 -0.8480016535738102 1103 60-61 -0.8127888763703055 1103 62-63 -1.0029380965784753 1103 64-65 -0.8500565327195133 1103 66-67 -0.811612098363824 1103 68-69 -0.8208312740980546 1103 70-71 -0.8105974931595838 1103 72-73 -1.0823214222975395 1103 74-75 -0.9305972029918479 1103 76-77 -0.9471341325693885 1103 78-79 -0.91678185421463 1103 80-81 -0.9691348993367583 1103 82-83 -0.9843923373413119 1103 84-85 -0.972875820300473 1103 86-87 -1.0628020014893522 1103 88-89 -1.1469263399150549 1103 90-91 -1.1606880474748529 1103 92-93 -1.235923477559158 1103 94-95 -1.4020859561608 1103 96-97 -1.5204930607671479 1103 98-99 -1.3955192494043906 1103 100 -1.4594860832456042 1104 1 -0.33920588789590056 1104 2 -0.43789217059442365 1104 3 -0.4318138969769727 1104 4 -0.34000033674904273 1104 5 -0.23638375472224737 1104 6 -0.2495220307112831 1104 7 -0.28595693270058575 1104 8 -0.25916431959268493 1104 9 -0.45406038413686645 1104 10-11 -0.35243180679781716 1104 12-13 -0.4645646364420841 1104 14-15 -0.557626164137055 1104 16-17 -0.5645940016241084 1104 18-19 -0.5845161374049965 1104 20-21 -0.6057610392189048 1104 22-23 -0.5196185622420515 1104 24-25 -0.6009582766367316 1104 26-27 -0.6187510074728735 1104 28-29 -0.5987913361569568 1104 30-31 -0.656142561006213 1104 32-33 -0.8080005363453964 1104 34-35 -0.7327147731092722 1104 36-37 -0.7563763158413863 1104 38-39 -0.7571081122209691 1104 40-41 -0.7185662977061895 1104 42-43 -0.7632174612389306 1104 44-45 -0.8875677642754809 1104 46-47 -0.8110274842456064 1104 48-49 -0.7317981635454558 1104 50-51 -0.7211750315482917 1104 52-53 -0.6212392128478967 1104 54-55 -0.6583193933743487 1104 56-57 -0.5993461883993376 1104 58-59 -0.624781939758698 1104 60-61 -0.5470732643335765 1104 62-63 -0.6199946662727243 1104 64-65 -0.7057115657585697 1104 66-67 -0.6614076230355579 1104 68-69 -0.4989545211626307 1104 70-71 -0.5887165035670989 1104 72-73 -0.6255797331353179 1104 74-75 -0.6335122272731688 1104 76-77 -0.558495220474601 1104 78-79 -0.6540438026365045 1104 80-81 -0.7078299777707961 1104 82-83 -0.7373002677283971 1104 84-85 -0.8118471317455977 1104 86-87 -0.8322072925430462 1104 88-89 -0.7150768675608035 1104 90-91 -0.6043667230654073 1104 92-93 -0.7822780569417311 1104 94-95 -0.839918527706562 1104 96-97 -0.980890862443399 1104 98-99 -0.8767349850690742 1104 100 -0.9968927760721122 1105 1 -0.4114670932217699 1105 2 -0.38740786034954056 1105 3 -0.31912933376312935 1105 4 -0.1698414423117427 1105 5 -0.24171719864310148 1105 6 -0.21350830223190087 1105 7 -0.21887335107121686 1105 8 -0.2018418058060547 1105 9 -0.4076471972959368 1105 10-11 -0.3884872760570417 1105 12-13 -0.4272364790856571 1105 14-15 -0.4883824475668703 1105 16-17 -0.5365370754944792 1105 18-19 -0.5748835554357541 1105 20-21 -0.5507262988095931 1105 22-23 -0.5694919785941579 1105 24-25 -0.5599073285440497 1105 26-27 -0.6174255438842664 1105 28-29 -0.6442308719778467 1105 30-31 -0.6509330412235244 1105 32-33 -0.628350509795375 1105 34-35 -0.6199566451516958 1105 36-37 -0.6571477719995968 1105 38-39 -0.7125670759858238 1105 40-41 -0.7607311927186657 1105 42-43 -0.6659875705132237 1105 44-45 -0.7268455496160868 1105 46-47 -0.6863226763027797 1105 48-49 -0.6885769473291177 1105 50-51 -0.7487703208804177 1105 52-53 -0.6284626035515188 1105 54-55 -0.5946911189093314 1105 56-57 -0.6023802833140834 1105 58-59 -0.5263310906538692 1105 60-61 -0.587588650273517 1105 62-63 -0.6893209087821504 1105 64-65 -0.5289645518470749 1105 66-67 -0.5958718580388549 1105 68-69 -0.5076514966239216 1105 70-71 -0.5777445384057742 1105 72-73 -0.6181566898808697 1105 74-75 -0.6947283354277687 1105 76-77 -0.6072736761018085 1105 78-79 -0.568756787444844 1105 80-81 -0.6345103596944242 1105 82-83 -0.679581730404653 1105 84-85 -0.6445936840743833 1105 86-87 -0.6583400498596319 1105 88-89 -0.7322890522624022 1105 90-91 -0.644641725386844 1105 92-93 -0.786827170874222 1105 94-95 -0.8540789475304535 1105 96-97 -0.8630385923489996 1105 98-99 -0.931578060472031 1105 100 -0.9656901457719123 1106 1 -0.3650933466467876 1106 2 -0.42535670738867637 1106 3 -0.4188692044953264 1106 4 -0.35239289680089314 1106 5 -0.33981983416331474 1106 6 -0.3796791827488235 1106 7 -0.44313346585116875 1106 8 -0.42271730631584603 1106 9 -0.45017637672247446 1106 10-11 -0.4806327811123836 1106 12-13 -0.4666857960742803 1106 14-15 -0.6285618614248563 1106 16-17 -0.6135209595772224 1106 18-19 -0.633901279503867 1106 20-21 -0.6325579427997639 1106 22-23 -0.5155172562627754 1106 24-25 -0.5815977522592064 1106 26-27 -0.541264189125819 1106 28-29 -0.6940132645368848 1106 30-31 -0.697385130823605 1106 32-33 -0.6508500615776498 1106 34-35 -0.643696175419521 1106 36-37 -0.7533767306675756 1106 38-39 -0.7502612266992585 1106 40-41 -0.8300525639208161 1106 42-43 -0.7087436839313241 1106 44-45 -0.6348305619398147 1106 46-47 -0.5039467601729157 1106 48-49 -0.6564820048549151 1106 50-51 -0.763505111811746 1106 52-53 -0.5736351085181326 1106 54-55 -0.6569788040594702 1106 56-57 -0.5186387247478734 1106 58-59 -0.5327135118264081 1106 60-61 -0.58586312975239 1106 62-63 -0.6030593886811673 1106 64-65 -0.6312394333636675 1106 66-67 -0.6525247491789941 1106 68-69 -0.6655345688440164 1106 70-71 -0.6377779147800808 1106 72-73 -0.540002531959729 1106 74-75 -0.594143098904631 1106 76-77 -0.7232773487706687 1106 78-79 -0.6669290732827946 1106 80-81 -0.7583118979414323 1106 82-83 -0.6604279111369102 1106 84-85 -0.5342076010988706 1106 86-87 -0.7242745792853498 1106 88-89 -0.7259378161775274 1106 90-91 -0.6789711826178184 1106 92-93 -0.7106319688864922 1106 94-95 -0.8179997388565816 1106 96-97 -0.8839119921211811 1106 98-99 -0.7975695271309711 1106 100 -0.8328760809713991 1107 1 -0.580991386442264 1107 2 -0.6008834005082662 1107 3 -0.5760192990044324 1107 4 -0.4338518708122763 1107 5 -0.4731899926775469 1107 6 -0.428437345837537 1107 7 -0.3884439773425896 1107 8 -0.4194980484272932 1107 9 -0.543243472475055 1107 10-11 -0.5213852569161403 1107 12-13 -0.5999788343550136 1107 14-15 -0.721471106750748 1107 16-17 -0.6758302648033023 1107 18-19 -0.7228113899269673 1107 20-21 -0.7693557849849668 1107 22-23 -0.7678910113026163 1107 24-25 -0.7964971981788764 1107 26-27 -0.8539300003218457 1107 28-29 -0.8343514893835646 1107 30-31 -0.8038857307234721 1107 32-33 -0.8375221550374619 1107 34-35 -0.8148464838681306 1107 36-37 -0.816071170101182 1107 38-39 -0.8572402809587842 1107 40-41 -0.8823296295128102 1107 42-43 -0.977626646739111 1107 44-45 -0.9512054540630785 1107 46-47 -0.8680065797553951 1107 48-49 -0.8660600469032929 1107 50-51 -0.8617093777711702 1107 52-53 -0.7971746427492761 1107 54-55 -0.6747006350051734 1107 56-57 -0.6207192612604402 1107 58-59 -0.691572374471427 1107 60-61 -0.6896497404011015 1107 62-63 -0.6701727241021729 1107 64-65 -0.679462783617474 1107 66-67 -0.6239583119477103 1107 68-69 -0.5867565915665764 1107 70-71 -0.7053384541633747 1107 72-73 -0.6474331903811716 1107 74-75 -0.5821630615147875 1107 76-77 -0.5318506330717696 1107 78-79 -0.5700146584946175 1107 80-81 -0.5766735250971777 1107 82-83 -0.6082362453377108 1107 84-85 -0.5668006520306257 1107 86-87 -0.7288536784592381 1107 88-89 -0.6435329788459043 1107 90-91 -0.7213366997655548 1107 92-93 -0.5312888120087074 1107 94-95 -0.7163485720278082 1107 96-97 -0.7499829543740759 1107 98-99 -0.8844581140045271 1107 100 -0.8828694609159555 1108 1 -0.44963334120388154 1108 2 -0.3524742024277714 1108 3 -0.45706405350345847 1108 4 -0.31898188723962306 1108 5 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0.842259618832621 2209 16-17 0.8442180764806295 2209 18-19 0.8765691118129055 2209 20-21 0.8449153909966327 2209 22-23 0.87334793627425 2209 24-25 0.9348039965836179 2209 26-27 1.0185861398319176 2209 28-29 1.0227439507901934 2209 30-31 1.0114851465810233 2209 32-33 0.9273622234324179 2209 34-35 0.9713770042808747 2209 36-37 0.9565482577032185 2209 38-39 0.9378438267118057 2209 40-41 1.0498631575216706 2209 42-43 1.0689416909264295 2209 44-45 1.024832141071009 2209 46-47 0.9727381646694155 2209 48-49 1.0478134098749692 2209 50-51 1.0334206484849702 2209 52-53 1.0175145411829618 2209 54-55 0.955716641924532 2209 56-57 0.9140693102162558 2209 58-59 0.9748271380522908 2209 60-61 0.903146266090495 2209 62-63 0.9295445053951141 2209 64-65 0.9347511826496557 2209 66-67 0.9292669685826311 2209 68-69 0.9123908826707705 2209 70-71 0.9940417402574582 2209 72-73 0.8842948676729847 2209 74-75 0.9082430642868857 2209 76-77 0.9368163418814177 2209 78-79 0.9302115909158921 2209 80-81 0.944595313808545 2209 82-83 0.8905786983234556 2209 84-85 0.9354883056578878 2209 86-87 0.977801468865664 2209 88-89 1.0186039902309432 2209 90-91 1.066618929512419 2209 92-93 1.0704927619558866 2209 94-95 1.1196370810676797 2209 96-97 1.1319143980525332 2209 98-99 1.0681277807404648 2209 100 1.2069123718795538 2210 1 0.6271939093716696 2210 2 0.6241006948015553 2210 3 0.6272857248189148 2210 4 0.5666973405053497 2210 5 0.5266231297429158 2210 6 0.5069004382066993 2210 7 0.5602787290033078 2210 8 0.540490863252387 2210 9 0.6952082420074603 2210 10-11 0.6703963193660272 2210 12-13 0.7083565794118485 2210 14-15 0.9152578468521995 2210 16-17 0.8709288737358705 2210 18-19 0.8647027184194727 2210 20-21 0.9036572448612574 2210 22-23 0.9325335301446742 2210 24-25 0.9557462435978081 2210 26-27 0.9753893974899555 2210 28-29 0.9474553609129472 2210 30-31 1.0450209270049982 2210 32-33 1.0214074502052668 2210 34-35 1.0590579343943745 2210 36-37 0.9945512458746038 2210 38-39 1.0463599475621592 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0.5238504418262835 2211 4 0.4342099038371501 2211 5 0.40304493357208315 2211 6 0.41629960734029936 2211 7 0.4262165018291597 2211 8 0.427165164479419 2211 9 0.511744011048954 2211 10-11 0.5007034185975527 2211 12-13 0.5446652905145228 2211 14-15 0.7807890552060854 2211 16-17 0.7399128983347723 2211 18-19 0.7151223794605812 2211 20-21 0.7828773845443777 2211 22-23 0.8124298695468539 2211 24-25 0.7672522014693683 2211 26-27 0.7789622779944736 2211 28-29 0.8272559125096279 2211 30-31 0.8425488265956176 2211 32-33 0.8399910084512143 2211 34-35 0.84901425812523 2211 36-37 0.8115996931561327 2211 38-39 0.9075459444616811 2211 40-41 0.8885334235724116 2211 42-43 0.9038557339226472 2211 44-45 0.9456781828187601 2211 46-47 0.9183343867958911 2211 48-49 0.9229193329924854 2211 50-51 0.8411546277076454 2211 52-53 0.7257295211985735 2211 54-55 0.7458269188852285 2211 56-57 0.734748642655596 2211 58-59 0.66541371166403 2211 60-61 0.7735255085300068 2211 62-63 0.724112664711889 2211 64-65 0.75311482131346 2211 66-67 0.7523780911927602 2211 68-69 0.8247081895559205 2211 70-71 0.7710914353605531 2211 72-73 0.7919304458380552 2211 74-75 0.8136025000054232 2211 76-77 0.7855124359547574 2211 78-79 0.7417461628421798 2211 80-81 0.7592357611715208 2211 82-83 0.8018929268729558 2211 84-85 0.7844791850118149 2211 86-87 0.7915458571212355 2211 88-89 0.8558222635112642 2211 90-91 0.841847790583035 2211 92-93 0.9430932157091938 2211 94-95 0.9749534784549532 2211 96-97 1.0473861746169355 2211 98-99 0.9856334821147819 2211 100 1.205264757596538 2212 1 0.4608707819182811 2212 2 0.40863926566113307 2212 3 0.45535732539740437 2212 4 0.3695358703370033 2212 5 0.260715862880069 2212 6 0.3258736684445722 2212 7 0.42211076064428 2212 8 0.421992329819723 2212 9 0.6096832566963926 2212 10-11 0.5978306632755945 2212 12-13 0.6094368071253413 2212 14-15 0.735316233163914 2212 16-17 0.7441356236396572 2212 18-19 0.7224775064843172 2212 20-21 0.7247467183934191 2212 22-23 0.7049552664856336 2212 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0.9370012216287797 2212 92-93 0.9732189408440242 2212 94-95 1.027867942684793 2212 96-97 1.1943800885471596 2212 98-99 1.1654341041465237 2212 100 1.2534761223899196 2213 1 0.4489164778157395 2213 2 0.4479116162772918 2213 3 0.4561212648753461 2213 4 0.35422111471790174 2213 5 0.3604322314751869 2213 6 0.3457788893932445 2213 7 0.39951372607987423 2213 8 0.4666366017326382 2213 9 0.50488447662444 2213 10-11 0.552635385381798 2213 12-13 0.5470775752314623 2213 14-15 0.7268667961854476 2213 16-17 0.6394280735153544 2213 18-19 0.6885227251528789 2213 20-21 0.7051296712612469 2213 22-23 0.758766536450608 2213 24-25 0.6913725135149349 2213 26-27 0.6811478715901913 2213 28-29 0.7390474686460706 2213 30-31 0.807306864874576 2213 32-33 0.8721342214670997 2213 34-35 0.8222233252067142 2213 36-37 0.9111410206032815 2213 38-39 0.8757065501872532 2213 40-41 0.9639559530062556 2213 42-43 0.9194116231912588 2213 44-45 0.947298549439914 2213 46-47 0.8471896586914411 2213 48-49 0.8650073348853837 2213 50-51 0.8719738987327972 2213 52-53 0.7427095250575917 2213 54-55 0.7502683589341288 2213 56-57 0.7364646485821496 2213 58-59 0.7749031553381585 2213 60-61 0.7257523330973257 2213 62-63 0.7413571840911501 2213 64-65 0.7199857708370345 2213 66-67 0.7220695859932889 2213 68-69 0.729478024963008 2213 70-71 0.7631529768989083 2213 72-73 0.7541714054809958 2213 74-75 0.7011659309152023 2213 76-77 0.7030813455487248 2213 78-79 0.8051729354394617 2213 80-81 0.7658209652736367 2213 82-83 0.8069674436677978 2213 84-85 0.7964184297139347 2213 86-87 0.8758535993188659 2213 88-89 0.9192617117274864 2213 90-91 0.9061547264704579 2213 92-93 0.9560247677190183 2213 94-95 0.9610810258142344 2213 96-97 0.9657616144785202 2213 98-99 1.0289943098045278 2213 100 1.1425601431783008 2214 1 0.4225781817004872 2214 2 0.42889437080514625 2214 3 0.38446495730546104 2214 4 0.3143248282785649 2214 5 0.3473735005787759 2214 6 0.41452982973621744 2214 7 0.36681235894657505 2214 8 0.3592398221356561 2214 9 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0.785657669270833 2214 76-77 0.8131830112513541 2214 78-79 0.7245796918663849 2214 80-81 0.727068762781439 2214 82-83 0.8135568238940181 2214 84-85 0.8822177881074786 2214 86-87 0.8031711059138047 2214 88-89 0.8504831879907258 2214 90-91 0.8524826244821107 2214 92-93 0.9080984120370061 2214 94-95 1.03210011519446 2214 96-97 1.0763652561551886 2214 98-99 1.1512766768274965 2214 100 1.2051228745026705 2215 1 0.24981778276200473 2215 2 0.11265415991152139 2215 3 0.2556963271025303 2215 4 0.16229792347658645 2215 5 0.1564335784845099 2215 6 0.14652549166007134 2215 7 0.2284666156208388 2215 8 0.17928048740246538 2215 9 0.2691335526813248 2215 10-11 0.29832269300225533 2215 12-13 0.31089772043021213 2215 14-15 0.4692210739954774 2215 16-17 0.47117449905235986 2215 18-19 0.41495195272566576 2215 20-21 0.4872923711355668 2215 22-23 0.43635554486476025 2215 24-25 0.4584682598813359 2215 26-27 0.5633702925527402 2215 28-29 0.4831229926952503 2215 30-31 0.5368244202637271 2215 32-33 0.5670756104950456 2215 34-35 0.5554272952522084 2215 36-37 0.6397061749367836 2215 38-39 0.631094993424945 2215 40-41 0.5751683891617603 2215 42-43 0.4867349134627901 2215 44-45 0.6311422698656557 2215 46-47 0.6992832361010599 2215 48-49 0.6732464094706572 2215 50-51 0.48944873823598556 2215 52-53 0.4987637495752182 2215 54-55 0.5529858981202054 2215 56-57 0.536327398826181 2215 58-59 0.5356376326409347 2215 60-61 0.5419661908681164 2215 62-63 0.5139293860813581 2215 64-65 0.482719845968397 2215 66-67 0.5088055970339482 2215 68-69 0.5097675747104873 2215 70-71 0.5994829046225689 2215 72-73 0.5847272869687927 2215 74-75 0.5707624361600949 2215 76-77 0.549116916867149 2215 78-79 0.5329322407169634 2215 80-81 0.5948468690953597 2215 82-83 0.6841919810296595 2215 84-85 0.5541037516023302 2215 86-87 0.5903309713226434 2215 88-89 0.6837235600512628 2215 90-91 0.7730521816466336 2215 92-93 0.7392246015348825 2215 94-95 0.9379645763366575 2215 96-97 0.8868323757960219 2215 98-99 0.8938520653803614 2215 100 0.9858260323249546 2216 1 0.1710027604647486 2216 2 0.0673200460615675 2216 3 0.11333789086398482 2216 4 0.049872679111430784 2216 5 0.008788578960967186 2216 6 0.09210129510023535 2216 7 0.12333351232032896 2216 8 0.14932596051976788 2216 9 0.16147487963286977 2216 10-11 0.2621277410740248 2216 12-13 0.11052398407869646 2216 14-15 0.32148981107634 2216 16-17 0.04468715234234111 2216 18-19 0.21858075754322925 2216 20-21 0.3000801194695413 2216 22-23 0.3494058550327779 2216 24-25 0.3517426241469437 2216 26-27 0.2869631998049371 2216 28-29 0.3198948111206832 2216 30-31 0.3264097810928348 2216 32-33 0.3736511885488838 2216 34-35 0.27728638538054184 2216 36-37 0.27110394567598206 2216 38-39 0.4608097469575725 2216 40-41 0.47898112913443924 2216 42-43 0.43798145157509083 2216 44-45 0.3648868177605209 2216 46-47 0.33547557220941115 2216 48-49 0.4091218946194246 2216 50-51 0.4346207299138527 2216 52-53 0.22698396397945686 2216 54-55 0.19717299667929922 2216 56-57 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0.32898847365565587 2311 7 0.4011136509321602 2311 8 0.4258039175484143 2311 9 0.5482552713079514 2311 10-11 0.5963110881682994 2311 12-13 0.6335205961910262 2311 14-15 0.7445776285642509 2311 16-17 0.7412069742228624 2311 18-19 0.6705663188637558 2311 20-21 0.7201337884916796 2311 22-23 0.7924621744543501 2311 24-25 0.8376523266028499 2311 26-27 0.8635769411121927 2311 28-29 0.7844717219638753 2311 30-31 0.8356417545585089 2311 32-33 0.8530588589168744 2311 34-35 0.9478151268553248 2311 36-37 0.8800326927078075 2311 38-39 0.9162603770596363 2311 40-41 0.8872042728083542 2311 42-43 1.0011268162090872 2311 44-45 0.9608275043473569 2311 46-47 1.0120340200590832 2311 48-49 1.0080343037715735 2311 50-51 1.0218384524771764 2311 52-53 0.9695889010101126 2311 54-55 0.9486611166445371 2311 56-57 0.8429922267934558 2311 58-59 0.8568751060249653 2311 60-61 0.934121427670938 2311 62-63 0.8970511994900008 2311 64-65 0.9255404870526149 2311 66-67 0.8927737681207759 2311 68-69 0.862740300131513 2311 70-71 0.9036913920607432 2311 72-73 0.8005495597474024 2311 74-75 0.9396995423736598 2311 76-77 0.9414732067726064 2311 78-79 1.0134200578427262 2311 80-81 1.0216982871648064 2311 82-83 0.9636330397295474 2311 84-85 0.9955576642501676 2311 86-87 1.0159474321702042 2311 88-89 1.000515178469442 2311 90-91 1.084785202226211 2311 92-93 1.005255293870885 2311 94-95 1.12377509193065 2311 96-97 1.0625699706615457 2311 98-99 1.104456955782716 2311 100 1.161750714068777 2312 1 0.4802775699644286 2312 2 0.4667531210515641 2312 3 0.544786039749436 2312 4 0.3561154745507693 2312 5 0.3715583902041857 2312 6 0.3792798687846144 2312 7 0.4166667117421099 2312 8 0.42961360991081676 2312 9 0.5417683385125827 2312 10-11 0.5397645866663439 2312 12-13 0.5895839332564989 2312 14-15 0.7598578741103452 2312 16-17 0.715809591623632 2312 18-19 0.6836123695003451 2312 20-21 0.6948926008311531 2312 22-23 0.69127301317328 2312 24-25 0.6916399003168081 2312 26-27 0.7778369008302235 2312 28-29 0.7983391894590923 2312 30-31 0.8194024366736841 2312 32-33 0.7430589625476998 2312 34-35 0.8552350959343542 2312 36-37 0.7887042891591207 2312 38-39 0.8587966001906722 2312 40-41 0.8317155900307114 2312 42-43 0.800346844547839 2312 44-45 0.8533085321825453 2312 46-47 0.885178903598721 2312 48-49 0.8600332472125913 2312 50-51 0.8824210345227144 2312 52-53 0.777897970232786 2312 54-55 0.8063444838931915 2312 56-57 0.7831171556482772 2312 58-59 0.7063595997406367 2312 60-61 0.784573669241226 2312 62-63 0.7124794208148231 2312 64-65 0.7162524131963579 2312 66-67 0.7587988578077827 2312 68-69 0.7887361412080409 2312 70-71 0.7151501370354296 2312 72-73 0.7261229626217656 2312 74-75 0.7073921993171837 2312 76-77 0.7504113578552207 2312 78-79 0.6875016760503421 2312 80-81 0.7913890580274163 2312 82-83 0.8445271911626016 2312 84-85 0.8427111062694088 2312 86-87 0.8602393562917179 2312 88-89 0.8418433562170264 2312 90-91 0.9593452644389302 2312 92-93 0.9859662112779297 2312 94-95 0.9520184050212741 2312 96-97 0.9965711024241877 2312 98-99 1.0613996692834782 2312 100 1.0359794785331786 2313 1 0.4923052696719026 2313 2 0.46147946260566286 2313 3 0.520810826563654 2313 4 0.40462288662599377 2313 5 0.38270005202611657 2313 6 0.4447248089584619 2313 7 0.4829601663070662 2313 8 0.45973439238385794 2313 9 0.5291170776457292 2313 10-11 0.5436375909221525 2313 12-13 0.5536106003286747 2313 14-15 0.7513769190320474 2313 16-17 0.7209310675421037 2313 18-19 0.7200568698584675 2313 20-21 0.7021872831348119 2313 22-23 0.618351358334138 2313 24-25 0.6539151776468799 2313 26-27 0.6971539957598765 2313 28-29 0.6971698175103995 2313 30-31 0.7938513540563363 2313 32-33 0.8059684737608919 2313 34-35 0.8189088215705453 2313 36-37 0.7804634820984546 2313 38-39 0.8065679557171705 2313 40-41 0.9017085701569627 2313 42-43 0.704577640837698 2313 44-45 0.831686774262927 2313 46-47 0.9014033445021781 2313 48-49 0.885814551369684 2313 50-51 0.739897709294361 2313 52-53 0.6583500530120148 2313 54-55 0.6015012990116233 2313 56-57 0.7057635983013313 2313 58-59 0.8002476068234969 2313 60-61 0.7240719507872129 2313 62-63 0.7598225016208957 2313 64-65 0.7208306784525931 2313 66-67 0.705388985932565 2313 68-69 0.7871173157060909 2313 70-71 0.7745331789785226 2313 72-73 0.7650054458426112 2313 74-75 0.7464760633181555 2313 76-77 0.8002280676703819 2313 78-79 0.8551267758372525 2313 80-81 0.8835554782686721 2313 82-83 0.8523647203923197 2313 84-85 0.919915531779548 2313 86-87 0.9867523521632897 2313 88-89 0.893133842929366 2313 90-91 0.977100342419039 2313 92-93 1.046259098226706 2313 94-95 1.0610372156264667 2313 96-97 1.1396797730453905 2313 98-99 1.1644236381840791 2313 100 1.1814110388473757 2314 1 0.2493522053226478 2314 2 0.1907097489529015 2314 3 0.2455955327626853 2314 4 0.23150021078345873 2314 5 0.18195935536520125 2314 6 0.21510408704399708 2314 7 0.29366327075450727 2314 8 0.3070645715363369 2314 9 0.3969631200157977 2314 10-11 0.39310518041693854 2314 12-13 0.381623912476158 2314 14-15 0.46402114346231116 2314 16-17 0.49331215814623164 2314 18-19 0.4480807327418077 2314 20-21 0.38759600140843986 2314 22-23 0.530467117004747 2314 24-25 0.5570922505882336 2314 26-27 0.5851720263436349 2314 28-29 0.5544824692473469 2314 30-31 0.5618785944317182 2314 32-33 0.5871112975147739 2314 34-35 0.6073036045256188 2314 36-37 0.5676629712471737 2314 38-39 0.6299080907073531 2314 40-41 0.5607123554073468 2314 42-43 0.5742622757081861 2314 44-45 0.6074525373360302 2314 46-47 0.47717403065364294 2314 48-49 0.35955879165970117 2314 50-51 0.4958106764493948 2314 52-53 0.5235055231085539 2314 54-55 0.489999430710121 2314 56-57 0.4299853592411367 2314 58-59 0.46715032993655115 2314 60-61 0.23773795856019575 2314 62-63 0.41091491789917 2314 64-65 0.5324819803577512 2314 66-67 0.4742110768308052 2314 68-69 0.49868054970812636 2314 70-71 0.482311947954031 2314 72-73 0.6013468832719937 2314 74-75 0.6302453920104156 2314 76-77 0.5680765379213923 2314 78-79 0.6553278815687342 2314 80-81 0.661444313274469 2314 82-83 0.6465414726379812 2314 84-85 0.6904536318734067 2314 86-87 0.6740389084434995 2314 88-89 0.53845708004771 2314 90-91 0.5769718307764755 2314 92-93 0.7178379155444183 2314 94-95 0.7684427820528832 2314 96-97 0.8127901299726119 2314 98-99 0.794547577691521 2314 100 0.8233160574523026 2315 1 0.2880282488652277 2315 2 0.38865273829834024 2315 3 0.30895255939946153 2315 4 0.2141162985462799 2315 5 0.2145814501462695 2315 6 0.19633128999365823 2315 7 0.18796219653846435 2315 8 0.225600469131372 2315 9 0.010606006701010529 2315 10-11 0.24876170020854005 2315 12-13 0.24969243645826822 2315 14-15 0.40804721965916 2315 16-17 0.2352178488795218 2315 18-19 0.3808141949694459 2315 20-21 0.2957675916328242 2315 22-23 0.3777959341757722 2315 24-25 0.4703248577081709 2315 26-27 0.5335825907769092 2315 28-29 0.4154316363829196 2315 30-31 0.47756314025930635 2315 32-33 0.5034131230482757 2315 34-35 0.40487445876215844 2315 36-37 0.5834636520308933 2315 38-39 0.6069880092781972 2315 40-41 0.7072983937128967 2315 42-43 0.7092285442093313 2315 44-45 0.6645464426455376 2315 46-47 0.6621936199277698 2315 48-49 0.5352198056557711 2315 50-51 0.5219774822578458 2315 52-53 0.3258305391926015 2315 54-55 0.5199160459386363 2315 56-57 0.4789740165102785 2315 58-59 0.46008116662199683 2315 60-61 0.5165338805503481 2315 62-63 0.5564657365070644 2315 64-65 0.5623898248578314 2315 66-67 0.46711790077288384 2315 68-69 0.5553569092159236 2315 70-71 0.5173537673565605 2315 72-73 0.5979253794080748 2315 74-75 0.2687439536766476 2315 76-77 0.49337452478846444 2315 78-79 0.5792702269828389 2315 80-81 0.5792855056695565 2315 82-83 0.49912908060827377 2315 84-85 0.5222742791711212 2315 86-87 0.45204405236074763 2315 88-89 0.44381092320571014 2315 90-91 0.6703979694393034 2315 92-93 0.7447518647780846 2315 94-95 0.6927542097930122 2315 96-97 0.7311164170129523 2315 98-99 0.8188853964549416 2315 100 0.9250874730417387 2316 1 0.07447156946386357 2316 2 0.05978685684374696 2316 3 0.04743452398773229 2316 4 -0.08703461659631984 2316 5 -0.06165552456601375 2316 6 -0.25258221883169796 2316 7 -0.14799826820151907 2316 8 -0.08195884142048016 2316 9 -0.11194441646717479 2316 10-11 -0.04266950978023232 2316 12-13 0.002457679771943333 2316 14-15 -0.20740327119830937 2316 16-17 0.05648869530819667 2316 18-19 0.021172783981619148 2316 20-21 0.10448734473058607 2316 22-23 0.02023791374744377 2316 24-25 0.028555575069241 2316 26-27 -0.10083831705484414 2316 28-29 -0.05144086326287578 2316 30-31 -0.6693404635303182 2316 32-33 -0.5305760745950749 2316 34-35 -0.6047089448958829 2316 36-37 -0.26876473328667316 2316 38-39 -0.15950533216081197 2316 40-41 0.13397539042897222 2316 42-43 0.1347301145319051 2316 44-45 -0.027681129795560366 2316 46-47 -0.117113596672489 2316 48-49 -0.030271018825672513 2316 50-51 0.14028069162700518 2316 52-53 -0.2669371247742589 2316 54-55 -0.11718708022248592 2316 56-57 -0.09576303669457076 2316 58-59 -0.4922604536157493 2316 60-61 -0.056813708520088824 2316 62-63 -0.02423641163300516 2316 64-65 -0.4179199744666846 2316 66-67 -0.7619088723484495 2316 68-69 -0.7446932685876462 2316 70-71 -0.4686583560339628 2316 72-73 -0.6761047058341276 2316 74-75 -0.4170050064366535 2316 76-77 -0.09578557235070662 2316 78-79 -0.11733569806674993 2316 80-81 -0.12106810217239072 2316 82-83 0.0595380789228237 2316 84-85 0.05836120451563076 2316 86-87 0.20370161994804192 2316 88-89 0.19941710182979477 2316 90-91 -0.32154655187301984 2316 92-93 -0.403613031348776 2316 94-95 -0.025492709853470075 2316 96-97 0.32349581627663326 2316 98-99 0.4300724291372866 2316 100 0.2524258570095519 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 163.0 1 409.0 2 234.0 3 249.0 4 225.0 5 210.0 6 287.0 7 747.0 8 1980.0 9 3369.0 10 5046.0 11 7969.0 12 11437.0 13 14107.0 14 16205.0 15 18161.0 16 21237.0 17 25843.0 18 31332.0 19 38143.0 20 45674.0 21 55747.0 22 62530.0 23 64045.0 24 63326.0 25 64466.0 26 70049.0 27 78821.0 28 92974.0 29 110323.0 30 134181.0 31 167004.0 32 210281.0 33 275064.0 34 381115.0 35 531678.0 36 776773.0 37 1100338.0 38 988877.0 39 236586.0 40 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.415408706106476 18.836346684495865 13.386482112234589 23.36176249716307 2 16.006874356713023 19.757882041427155 40.54882947203285 23.68641412982698 3 19.26019440308244 25.566754889386306 29.22225273382073 25.950797973710525 4 13.045397543945485 16.292372688292488 37.37581461337715 33.286415154384876 5 13.708240116896302 37.25445800344674 34.14758613969865 14.889715739958298 6 31.211086190379778 36.16630337323864 18.00196502742011 14.620645408961472 7 27.785743834972898 32.20007502506679 21.98588547108029 18.028295668880023 8 26.124517346601273 35.058291752706886 20.078994864492465 18.73819603619938 9 26.865185855626095 15.989793666682031 20.563136088358608 36.58188438933327 10-11 25.464979655236693 25.912907113266115 27.629879112782703 20.992234118714492 12-13 25.807234412532075 23.68131826239278 27.93352740337388 22.577919921701266 14-15 23.327496972278162 25.014923908223665 25.85526350381467 25.80231561568351 16-17 22.4967619710668 27.8464090633786 26.66204285161787 22.994786113936726 18-19 22.254561522526974 26.876847677455252 28.901621289450897 21.966969510566877 20-21 23.171716350134215 26.149367510646083 28.777358453522645 21.901557685697064 22-23 23.163736680303174 25.90302230850642 28.568123004647518 22.365118006542893 24-25 22.717919321111903 26.0675807563397 28.80630812870169 22.408191793846704 26-27 22.529275417047206 26.275264787251118 28.651659637901805 22.543800157799872 28-29 22.401591446326595 26.42407616517952 28.7682181941912 22.40611419430269 30-31 22.956625591101087 26.217095022129477 28.69399615188283 22.132283234886604 32-33 22.84573016372167 26.378694482627818 28.455478651256826 22.320096702393684 34-35 22.93601830727795 26.44056313635714 28.533770038923123 22.089648517441784 36-37 22.87950559241786 26.334376290788025 28.388305146079627 22.397812970714487 38-39 22.856967565852557 26.435600609312527 28.4256475698492 22.281784254985723 40-41 22.799555177322073 26.100409770146026 28.666887961887667 22.433147090644233 42-43 22.651724056018924 26.288855391033888 28.316622925347986 22.742797627599202 44-45 22.532892905833595 26.61434201434133 28.31281572670844 22.539949353116633 46-47 22.57645477143185 26.41533796588534 27.997699473422756 23.010507789260053 48-49 22.809995639846022 26.460682263289836 27.88325481725134 22.8460672796128 50-51 22.76626583835165 26.63470932569648 27.72409480545367 22.8749300304982 52-53 22.62766863171655 26.862870094914292 27.575042884653953 22.9344183887152 54-55 22.60327121378326 26.683168381918875 27.715475135015026 22.998085269282836 56-57 22.809410521862922 26.634213326888943 27.462017171764717 23.094358979483413 58-59 22.524955097434905 26.687800447925603 27.791039226491687 22.996205228147808 60-61 22.783776089618005 26.797121184286 27.60185346199747 22.81724926409853 62-63 22.785763566483652 26.734900653387495 27.53895794770709 22.940377832421767 64-65 22.50402919833175 26.570786924982976 27.618339047299155 23.30684482938612 66-67 22.654131432905928 26.489328152070655 27.489406863227185 23.367133551796236 68-69 22.414835013602623 26.554931359249206 27.64130057857177 23.388933048576398 70-71 22.550831015242338 26.34427801874704 27.65607471326937 23.448816252741256 72-73 22.65652516852093 26.662115387548607 27.35709711142797 23.324262332502496 74-75 22.680853876101963 26.780560755658577 27.281938532181982 23.256646836057477 76-77 22.593088536672827 26.594107746070307 27.284011183254954 23.528792534001912 78-79 22.595004445680257 26.54518617814647 27.31046732194904 23.549342054224237 80-81 22.54544013725531 26.764785653411753 27.22019073873786 23.469583470595072 82-83 22.820070198960163 26.536754881092666 27.45968959171216 23.183485328235008 84-85 22.857787758937462 26.65088848683749 27.068817407890496 23.422506346334554 86-87 22.52250712370582 26.82238717687372 27.32293116257967 23.33217453684079 88-89 22.695186351969713 26.376317026664843 27.323682947036488 23.604813674328962 90-91 22.904570302880632 26.31875952992052 27.305211777813604 23.471458389385244 92-93 22.924017274910184 26.415391260794735 27.195657092980248 23.464934371314833 94-95 22.58503449073993 26.299513587633133 27.35506700005962 23.760384921567322 96-97 22.67562575336187 26.21056250055342 27.29397456912457 23.81983717696014 98-99 22.608492085933545 26.32628180257906 27.13030974566705 23.934916365820353 100 22.9656026470757 26.201502163880175 26.778291270604406 24.054603918439728 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2263.0 1 1599.5 2 2281.0 3 3054.0 4 1917.5 5 1555.0 6 2224.0 7 3956.0 8 6288.5 9 7279.5 10 6295.0 11 5174.5 12 5269.0 13 5721.0 14 6182.5 15 6780.5 16 7325.0 17 7890.5 18 8432.0 19 8933.5 20 9572.0 21 10463.0 22 11729.0 23 13461.0 24 15727.5 25 18945.5 26 23510.5 27 28878.0 28 34547.0 29 42386.5 30 52870.0 31 63276.0 32 75880.0 33 89920.5 34 103209.0 35 116817.5 36 132465.5 37 149464.5 38 157775.5 39 159378.0 40 159180.5 41 158307.0 42 161801.5 43 170467.5 44 180230.5 45 193633.0 46 206215.0 47 213932.0 48 223041.0 49 229234.5 50 229671.0 51 228030.0 52 224658.0 53 218257.0 54 209356.0 55 195029.5 56 178339.5 57 158981.0 58 138977.5 59 122293.0 60 103617.5 61 84690.5 62 69033.5 63 55468.5 64 42329.0 65 31587.5 66 24047.5 67 18646.0 68 14887.0 69 11864.0 70 9587.0 71 7539.0 72 5914.5 73 4695.5 74 3694.5 75 2929.0 76 2243.5 77 1574.0 78 1081.0 79 725.0 80 492.5 81 380.5 82 297.5 83 207.5 84 144.5 85 112.0 86 87.0 87 75.0 88 65.0 89 50.0 90 32.0 91 29.0 92 23.0 93 14.5 94 15.0 95 13.0 96 10.0 97 6.5 98 2.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024949981769342672 2 0.1091736912954875 3 0.01801164414247491 4 0.018064207306314816 5 0.020412028624497345 6 0.0466760894898377 7 0.04658848421677119 8 0.039212120224570865 9 0.03402588805903334 10-11 0.037538859509000475 12-13 0.036627764669108755 14-15 0.03169558779546411 16-17 0.03808201220201285 18-19 0.037048269979828005 20-21 0.04797264753122208 22-23 0.04829678704156817 24-25 0.06758746817081417 26-27 0.05884446191877624 28-29 0.05120528210737638 30-31 0.04925168451799316 32-33 0.04367998915096298 34-35 0.045274405120773506 36-37 0.04079777566707474 38-39 0.046062852578372115 40-41 0.05472701408465017 42-43 0.056759456419793256 44-45 0.05984316203173449 46-47 0.06145509905615831 48-49 0.06118352270965212 50-51 0.055156279922676084 52-53 0.06565139163604425 54-55 0.051888603237295175 56-57 0.05658424587366023 58-59 0.05472701408465017 60-61 0.049952526702525264 62-63 0.05647911954598042 64-65 0.05467445092081026 66-67 0.05287854282294677 68-69 0.05909851721066914 70-71 0.059080996156055825 72-73 0.061814280675731005 74-75 0.05147685845388257 76-77 0.05251936120337406 78-79 0.06250636233295645 80-81 0.06300571238943557 82-83 0.05363194817131877 84-85 0.058502801353816844 86-87 0.056505401127900375 88-89 0.06499435208804541 90-91 0.0700929789805164 92-93 0.07419290576002918 94-95 0.07777596142844953 96-97 0.07417538470541589 98-99 0.08333889626817306 100 0.12688747750953627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5707419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.09053928955306 #Duplication Level Percentage of deduplicated Percentage of total 1 74.16351981764845 43.823623816363025 2 15.385065628950532 18.18223650039707 3 4.89474734589039 8.67699781064315 4 2.1498350480269863 5.081396494859872 5 1.0668032417035833 3.1518989434054077 6 0.6039951549228666 2.14142396596016 7 0.3713083759464201 1.5358568524181448 8 0.2701942425745178 1.2772738805328459 9 0.1890124709280942 1.005196395563285 >10 0.8339850173092944 8.40535745105202 >50 0.045585774502925565 1.8482509845333106 >100 0.02403723596377027 2.5673947586317 >500 0.0013486914797021187 0.5221343508384072 >1k 4.4956332196035155E-4 0.5936599774175875 >5k 0.0 0.0 >10k+ 1.1239083048972427E-4 1.187297817384153 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23302 0.40827561459917344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18792 0.3292556582931795 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11539 0.20217544918289687 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.752105461330244E-5 2 0.0 0.0 0.0 0.0 1.752105461330244E-5 3 0.0 0.0 0.0 0.0 1.752105461330244E-5 4 0.0 0.0 0.0 0.0 1.752105461330244E-5 5 0.0 0.0 0.0 0.0 1.752105461330244E-5 6 0.0 0.0 0.0 0.0 1.752105461330244E-5 7 0.0 0.0 0.0 0.0 1.752105461330244E-5 8 0.0 0.0 0.0 0.0 1.752105461330244E-5 9 0.0 0.0 0.0 1.752105461330244E-5 3.504210922660488E-5 10-11 0.0 0.0 0.0 1.752105461330244E-5 3.504210922660488E-5 12-13 0.0 0.0 0.0 1.752105461330244E-5 4.38026365332561E-5 14-15 0.0 0.0 0.0 1.752105461330244E-5 1.1388685498646588E-4 16-17 0.0 0.0 0.0 3.504210922660488E-5 1.4016843690641952E-4 18-19 0.0 0.0 0.0 3.504210922660488E-5 1.752105461330244E-4 20-21 0.0 0.0 0.0 5.256316383990732E-5 2.540552918928854E-4 22-23 0.0 0.0 0.0 2.8909740111949026E-4 3.329000376527464E-4 24-25 0.0 0.0 0.0 8.322500941318659E-4 3.854632014926537E-4 26-27 0.0 0.0 0.0 0.001673260715570383 4.4678689263921224E-4 28-29 0.0 0.0 0.0 0.0045992768359918905 5.431526930123757E-4 30-31 0.0 0.0 0.0 0.01522579645895982 5.781948022389805E-4 32-33 0.0 0.0 0.0 0.041813996834646275 5.781948022389805E-4 34-35 0.0 0.0 0.0 0.08236647773713476 6.482790206921902E-4 36-37 0.0 0.0 0.0 0.14800034831856573 6.482790206921902E-4 38-39 0.0 0.0 0.0 0.24524220142239428 6.570395479988415E-4 40-41 0.0 0.0 0.0 0.3529966872942043 6.833211299187952E-4 42-43 0.0 0.0 0.0 0.46025182310953516 7.183632391454001E-4 44-45 0.0 0.0 0.0 0.5894170377188007 7.183632391454001E-4 46-47 0.0 0.0 0.0 0.7421831128921847 7.183632391454001E-4 48-49 0.0 0.0 0.0 0.9054881024154701 7.446448210653538E-4 50-51 1.752105461330244E-5 0.0 0.0 1.0739793241042932 7.709264029853074E-4 52-53 1.752105461330244E-5 1.752105461330244E-5 0.0 1.2403680192395197 7.884474575986098E-4 54-55 1.752105461330244E-5 1.752105461330244E-5 0.0 1.4332835910592863 8.059685122119122E-4 56-57 1.752105461330244E-5 1.752105461330244E-5 0.0 1.6575268085276376 8.059685122119122E-4 58-59 1.752105461330244E-5 1.752105461330244E-5 0.0 1.9069565420026109 8.059685122119122E-4 60-61 1.752105461330244E-5 1.752105461330244E-5 0.0 2.15896187050574 8.234895668252146E-4 62-63 1.752105461330244E-5 1.752105461330244E-5 0.0 2.4132624571632117 8.410106214385172E-4 64-65 1.752105461330244E-5 1.752105461330244E-5 0.0 2.673747976099179 8.410106214385172E-4 66-67 1.752105461330244E-5 1.752105461330244E-5 0.0 2.96820681993034 8.410106214385172E-4 68-69 1.752105461330244E-5 1.752105461330244E-5 0.0 3.29144399596385 8.410106214385172E-4 70-71 1.752105461330244E-5 1.752105461330244E-5 0.0 3.631886847627623 8.410106214385172E-4 72-73 1.752105461330244E-5 1.752105461330244E-5 0.0 3.9602489321355243 8.672922033584709E-4 74-75 1.752105461330244E-5 1.752105461330244E-5 0.0 4.290932556379687 8.76052730665122E-4 76-77 1.752105461330244E-5 1.752105461330244E-5 0.0 4.657411695198828 8.848132579717732E-4 78-79 1.752105461330244E-5 1.752105461330244E-5 0.0 5.054964424374661 8.935737852784245E-4 80-81 1.752105461330244E-5 1.752105461330244E-5 0.0 5.468382468502838 8.935737852784245E-4 82-83 1.752105461330244E-5 1.752105461330244E-5 0.0 5.890079210935801 8.935737852784245E-4 84-85 1.752105461330244E-5 1.752105461330244E-5 0.0 6.309682187342474 8.935737852784245E-4 86-87 1.752105461330244E-5 1.752105461330244E-5 0.0 6.744379552298509 8.935737852784245E-4 88 1.752105461330244E-5 1.752105461330244E-5 0.0 7.0899473124366725 8.935737852784245E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10275 0.0 53.240612 1 GTATCAA 18555 0.0 40.86478 1 ATCAACG 23205 0.0 31.934006 3 CAACGCA 23255 0.0 31.64252 5 TATCAAC 23915 0.0 31.331076 2 TCAACGC 24205 0.0 30.575329 4 AACGCAG 24770 0.0 30.144547 6 GTACATG 24270 0.0 26.9783 1 ACGCAGA 27505 0.0 26.788542 7 GTGGTAT 4910 0.0 26.249086 1 TACATGG 24770 0.0 26.034025 2 CGCAGAG 28430 0.0 25.816874 8 ACATGGG 25355 0.0 24.852406 3 TACCTGG 3540 0.0 23.783712 2 CATGGGG 17395 0.0 23.582262 4 GCAGAGT 30885 0.0 23.446875 9 TGGTATC 5260 0.0 23.249708 2 ATGGGGG 11900 0.0 22.467821 5 GAGTACT 17840 0.0 21.690145 12-13 GTACTAG 1805 0.0 20.847689 1 >>END_MODULE